AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069431

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069431.t1 MTR_0334s0010 88.500 200 18 2 1 196 1 199 1.86e-106 304
MS.gene069431.t1 MTR_4g067200 50.746 201 66 5 1 197 1 172 2.29e-57 178
MS.gene069431.t1 MTR_4g066110 50.746 201 66 5 1 197 1 172 2.29e-57 178
MS.gene069431.t1 MTR_4g114870 47.917 144 72 3 1 141 1 144 2.58e-35 127
MS.gene069431.t1 MTR_4g114870 49.000 100 50 1 28 126 191 290 4.99e-27 105
MS.gene069431.t1 MTR_6g083240 44.516 155 78 4 9 155 8 162 2.73e-35 122
MS.gene069431.t1 MTR_4g114830 47.287 129 63 5 17 141 17 144 1.40e-31 112
MS.gene069431.t1 MTR_3g099580 43.810 105 55 1 23 127 21 121 3.65e-25 97.1
MS.gene069431.t1 MTR_8g099220 37.984 129 77 3 1 127 1 128 1.18e-23 91.3
MS.gene069431.t1 MTR_2g090580 33.898 177 110 4 6 178 5 178 4.24e-23 92.0
MS.gene069431.t1 MTR_2g101300 37.762 143 84 4 11 151 9 148 4.49e-23 91.7
MS.gene069431.t1 MTR_3g099570 37.692 130 71 2 23 152 21 140 9.24e-23 90.5
MS.gene069431.t1 MTR_2g079030 35.196 179 102 7 3 172 2 175 7.08e-21 89.7
MS.gene069431.t1 MTR_2g088990 35.878 131 79 2 8 137 5 131 1.01e-20 86.3
MS.gene069431.t1 MTR_1g090420 27.404 208 133 4 1 191 1 207 1.57e-20 85.5
MS.gene069431.t1 MTR_8g007020 34.884 172 91 5 7 163 1 166 2.18e-20 84.3
MS.gene069431.t1 MTR_5g006040 30.323 155 103 4 8 160 7 158 7.79e-20 82.8
MS.gene069431.t1 MTR_8g463180 35.461 141 78 5 10 148 7 136 1.40e-19 84.7
MS.gene069431.t1 MTR_3g099980 38.281 128 71 4 1 126 1 122 1.53e-19 82.8
MS.gene069431.t1 MTR_3g099680 38.281 128 71 4 1 126 1 122 2.60e-19 82.4
MS.gene069431.t1 MTR_1g014120 32.886 149 88 4 4 148 9 149 1.86e-18 79.3
MS.gene069431.t1 MTR_4g111640 29.193 161 106 3 9 167 13 167 2.69e-18 79.0
MS.gene069431.t1 MTR_6g080660 34.711 121 75 1 10 130 10 126 3.16e-18 79.3
MS.gene069431.t1 MTR_7g086230 31.013 158 103 3 10 165 7 160 4.99e-18 78.2
MS.gene069431.t1 MTR_3g105930 35.036 137 83 3 25 155 24 160 1.23e-17 77.0
MS.gene069431.t1 MTR_4g078410 34.694 147 83 5 24 163 21 161 1.70e-17 77.0
MS.gene069431.t1 MTR_2g043730 36.975 119 64 4 7 124 14 122 1.77e-17 75.1
MS.gene069431.t1 MTR_7g086280 30.380 158 104 3 10 165 7 160 2.32e-17 76.3
MS.gene069431.t1 MTR_8g007035 38.318 107 60 2 25 129 21 123 2.58e-17 76.6
MS.gene069431.t1 MTR_8g094990 33.333 123 78 3 7 126 2 123 6.70e-17 77.0
MS.gene069431.t1 MTR_8g095013 30.387 181 120 4 7 184 2 179 9.00e-17 77.0
MS.gene069431.t1 MTR_8g089110 32.800 125 76 3 1 124 1 118 1.93e-16 72.4
MS.gene069431.t1 MTR_1g090190 32.117 137 87 2 25 159 20 152 2.86e-16 73.6
MS.gene069431.t1 MTR_3g089035 38.095 105 57 3 21 124 26 123 4.88e-16 71.6
MS.gene069431.t1 MTR_7g086090 34.426 122 74 3 8 127 5 122 5.96e-16 73.9
MS.gene069431.t1 MTR_1g112700 28.485 165 104 6 7 168 14 167 6.31e-16 72.4
MS.gene069431.t1 MTR_6g022170 35.833 120 72 3 17 135 26 141 8.62e-16 72.0
MS.gene069431.t1 MTR_1g104800 27.273 154 109 2 12 164 9 160 1.26e-15 72.0
MS.gene069431.t1 MTR_2g090575 30.645 124 83 2 4 126 1 122 1.41e-15 73.9
MS.gene069431.t1 MTR_8g088830 36.792 106 58 4 21 124 21 119 5.82e-15 68.6
MS.gene069431.t1 MTR_4g077787 27.097 155 104 3 9 155 13 166 7.28e-15 69.7
MS.gene069431.t1 MTR_6g023760 36.893 103 60 3 25 126 23 121 2.76e-14 68.2
MS.gene069431.t1 MTR_3g099540 34.921 126 76 2 7 132 8 127 3.21e-14 67.0
MS.gene069431.t1 MTR_7g086100 32.773 119 74 3 10 126 7 121 3.32e-14 68.9
MS.gene069431.t1 MTR_4g124280 29.762 168 106 5 9 164 6 173 3.83e-14 68.9
MS.gene069431.t1 MTR_2g083250 29.897 194 92 6 20 181 18 199 7.53e-14 68.6
MS.gene069431.t1 MTR_1g072180 48.276 58 30 0 69 126 42 99 8.81e-14 64.7
MS.gene069431.t1 MTR_4g081100 27.500 200 123 5 4 183 6 203 1.45e-13 67.0
MS.gene069431.t1 MTR_3g089005 31.667 120 74 3 6 124 11 123 2.63e-13 64.3
MS.gene069431.t1 MTR_7g086140 29.839 124 80 4 9 129 6 125 7.93e-13 63.9
MS.gene069431.t1 MTR_7g086190 29.839 124 80 4 9 129 6 125 7.93e-13 63.9
MS.gene069431.t1 MTR_6g013420 33.103 145 82 7 9 149 18 151 3.34e-12 62.4
MS.gene069431.t1 MTR_7g090170 32.353 136 71 4 25 155 24 143 3.43e-12 62.0
MS.gene069431.t1 MTR_8g086360 27.979 193 120 8 11 194 5 187 4.60e-12 62.4
MS.gene069431.t1 MTR_4g112310 28.689 122 81 2 46 167 2 117 4.72e-12 61.2
MS.gene069431.t1 MTR_6g013200 30.939 181 99 8 1 178 10 167 6.79e-12 61.6
MS.gene069431.t1 MTR_7g086220 29.730 111 72 3 12 120 8 114 6.88e-12 61.2
MS.gene069431.t1 MTR_7g086160 28.800 125 81 4 9 129 6 126 9.14e-12 60.8
MS.gene069431.t1 MTR_4g130780 28.689 122 84 3 9 127 9 130 1.18e-11 61.2
MS.gene069431.t1 MTR_8g095020 28.571 119 81 3 9 126 6 121 1.60e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069431.t1 AT2G32300 40.714 140 77 4 5 143 4 138 1.57e-23 94.4
MS.gene069431.t1 AT2G32300 40.714 140 77 4 5 143 34 168 4.51e-23 94.0
MS.gene069431.t1 AT5G20230 41.912 136 66 4 27 161 24 147 5.52e-23 91.7
MS.gene069431.t1 AT5G53870 35.638 188 112 5 7 188 10 194 1.42e-22 93.6
MS.gene069431.t1 AT5G53870 35.638 188 112 5 7 188 10 194 2.43e-22 93.2
MS.gene069431.t1 AT2G25060 36.667 150 84 4 12 150 14 163 1.36e-21 87.4
MS.gene069431.t1 AT3G20570 33.511 188 113 6 5 182 3 188 3.28e-20 84.3
MS.gene069431.t1 AT4G28365 31.527 203 117 9 6 197 8 199 1.96e-19 82.4
MS.gene069431.t1 AT4G27520 31.081 148 93 4 3 143 5 150 2.34e-19 84.7
MS.gene069431.t1 AT3G17675 29.134 127 85 2 2 127 6 128 3.57e-19 79.7
MS.gene069431.t1 AT1G72230 39.669 121 68 2 46 164 37 154 1.31e-18 79.7
MS.gene069431.t1 AT4G31840 29.050 179 101 4 19 195 18 172 8.58e-18 77.4
MS.gene069431.t1 AT5G07475 30.000 150 96 3 1 148 1 143 1.26e-17 77.4
MS.gene069431.t1 AT3G60270 36.000 100 60 1 30 129 28 123 2.68e-17 76.3
MS.gene069431.t1 AT2G26720 33.582 134 78 4 9 138 14 140 8.80e-17 75.5
MS.gene069431.t1 AT2G44790 40.230 87 50 1 46 130 42 128 8.38e-16 72.8
MS.gene069431.t1 AT3G27200 28.000 125 84 3 4 126 1 121 1.52e-15 71.6
MS.gene069431.t1 AT5G26330 32.520 123 78 3 7 126 1 121 9.28e-15 69.7
MS.gene069431.t1 AT2G31050 34.815 135 78 4 8 138 12 140 1.16e-14 69.7
MS.gene069431.t1 AT1G79800 32.609 138 84 3 9 138 15 151 6.25e-14 67.4
MS.gene069431.t1 AT3G18590 32.787 122 73 4 9 126 10 126 9.98e-14 67.0
MS.gene069431.t1 AT1G22480 41.772 79 46 0 53 131 40 118 1.17e-13 66.2
MS.gene069431.t1 AT4G32490 34.711 121 72 4 20 138 23 138 1.37e-13 67.0
MS.gene069431.t1 AT5G57920 24.118 170 112 4 1 153 18 187 6.57e-13 65.1
MS.gene069431.t1 AT3G60280 34.286 105 65 1 25 129 21 121 6.62e-13 65.5
MS.gene069431.t1 AT5G57920 27.273 121 86 2 8 126 3 123 9.60e-13 64.3
MS.gene069431.t1 AT5G14345 31.132 106 71 2 35 139 23 127 1.06e-12 63.2
MS.gene069431.t1 AT4G30590 32.039 103 68 2 30 130 30 132 2.01e-12 63.5
MS.gene069431.t1 AT1G48940 29.412 119 78 3 10 126 11 125 6.39e-12 61.6
MS.gene069431.t1 AT5G15350 34.965 143 78 7 12 150 12 143 7.74e-12 61.6
MS.gene069431.t1 AT5G57920 31.313 99 66 2 30 126 24 122 9.48e-12 61.2
MS.gene069431.t1 AT2G27035 32.903 155 89 7 8 159 8 150 1.16e-11 60.8
MS.gene069431.t1 AT2G02850 30.000 120 74 4 8 124 14 126 6.02e-11 58.2

Find 55 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACTTTAACGCTTAGCTTT+TGG 0.290349 2.2:+30526176 None:intergenic
AATTGCAACATTAATCAAAA+TGG 0.321277 2.2:-30526292 MS.gene069431:CDS
GGTGATGGAGGAGTTGCATC+AGG 0.366204 2.2:+30526031 None:intergenic
CTGTTAAGAGTAACTCTTGC+TGG 0.373715 2.2:+30526250 None:intergenic
CTGGTCCTGTTGTTATGACT+TGG 0.377013 2.2:+30526269 None:intergenic
ACCATTCAAGGTCATTGTTC+CGG 0.394011 2.2:-30526202 MS.gene069431:CDS
ATGATAACCGCAATGAGAAC+AGG 0.411992 2.2:+30525977 None:intergenic
GGAGTAGATGTAGGAGGAGT+AGG 0.442205 2.2:+30526127 None:intergenic
AGAGTTACTCTTAACAGAAC+TGG 0.466599 2.2:-30526244 MS.gene069431:CDS
ACCGGAACAATGACCTTGAA+TGG 0.487199 2.2:+30526201 None:intergenic
GTAGGAGGAGTAGGAGAAGG+AGG 0.487629 2.2:+30526136 None:intergenic
CCTCCGTTGGTAGGTGGAGT+AGG 0.491660 2.2:+30526082 None:intergenic
TTGTTTGAATGTATTATTGG+CGG 0.499396 2.2:+30527656 None:intergenic
CATACTGTTGGAGGCACCAC+TGG 0.499434 2.2:-30527723 MS.gene069431:CDS
AATGGCCAAGTCATAACAAC+AGG 0.504661 2.2:-30526274 MS.gene069431:CDS
ACGAGAGTGGTAGCTGAGCC+TGG 0.506000 2.2:+30526010 None:intergenic
AGGAGTTGCATCAGGGCCGG+TGG 0.506469 2.2:+30526039 None:intergenic
TAAAACGAAGCGCCGCCACT+TGG 0.519955 2.2:+30527690 None:intergenic
GTAGGAGGAGGAGTAGATGT+AGG 0.523768 2.2:+30526118 None:intergenic
TTCAGCAACAGTGTGGCCTC+CGG 0.526784 2.2:+30526336 None:intergenic
TCTACTCCTCCTCCTACATC+AGG 0.528805 2.2:-30526112 MS.gene069431:CDS
AGGAGAAGGAGGAGAAGTCG+AGG 0.539764 2.2:+30526147 None:intergenic
CGGCGAAGGTGTTCCTCCGT+TGG 0.540474 2.2:+30526069 None:intergenic
CACTGTTGCTGAAGTAAGCA+AGG 0.543847 2.2:-30526326 MS.gene069431:CDS
GAAGGTGTTCCTCCGTTGGT+AGG 0.546863 2.2:+30526073 None:intergenic
TCAGGGCCGGTGGGAGATGA+CGG 0.548510 2.2:+30526049 None:intergenic
GAGGCAGCAGATCATACTGT+TGG 0.551162 2.2:-30527735 MS.gene069431:CDS
GATGTAGGAGGAGTAGGAGA+AGG 0.553864 2.2:+30526133 None:intergenic
GTAGGAGTAGTTCCTGATGT+AGG 0.559933 2.2:+30526100 None:intergenic
GTGGTAGCTGAGCCTGGTGA+TGG 0.561174 2.2:+30526016 None:intergenic
TTCAAACAAAACGATGTCCT+AGG 0.564087 2.2:-30527642 MS.gene069431:intron
TGGAGGAGTTGCATCAGGGC+CGG 0.566001 2.2:+30526036 None:intergenic
GGTGTTCCTCCGTTGGTAGG+TGG 0.575863 2.2:+30526076 None:intergenic
GATGCAACTCCTCCATCACC+AGG 0.578460 2.2:-30526028 MS.gene069431:CDS
GGAGGAGGAGTAGATGTAGG+AGG 0.578805 2.2:+30526121 None:intergenic
GGAGTTGCATCAGGGCCGGT+GGG 0.580673 2.2:+30526040 None:intergenic
GTAGTTCCTGATGTAGGAGG+AGG 0.581223 2.2:+30526106 None:intergenic
CCGGTGGGAGATGACGGCGA+AGG 0.587429 2.2:+30526055 None:intergenic
GCAGCAGATCATACTGTTGG+AGG 0.591168 2.2:-30527732 MS.gene069431:CDS
TACGTTCCCTGTTCTCATTG+CGG 0.597691 2.2:-30525984 MS.gene069431:CDS
ATTCAAGGTCATTGTTCCGG+TGG 0.607111 2.2:-30526199 MS.gene069431:CDS
GGTTGGAGTGTTCCAAGTGG+CGG 0.608033 2.2:-30527702 MS.gene069431:CDS
CTGTTGGAGGCACCACTGGT+TGG 0.613559 2.2:-30527719 MS.gene069431:CDS
GTGATGGAGGAGTTGCATCA+GGG 0.617363 2.2:+30526032 None:intergenic
TGCTTACTTCAGCAACAGTG+TGG 0.617452 2.2:+30526329 None:intergenic
GGAGTAGTTCCTGATGTAGG+AGG 0.617793 2.2:+30526103 None:intergenic
GTAGCTGAGCCTGGTGATGG+AGG 0.629395 2.2:+30526019 None:intergenic
CCTTCGCCGTCATCTCCCAC+CGG 0.651978 2.2:-30526055 MS.gene069431:CDS
TGATAACCGCAATGAGAACA+GGG 0.653429 2.2:+30525978 None:intergenic
ACTTGGAACACTCCAACCAG+TGG 0.663713 2.2:+30527707 None:intergenic
ACTCCTACTCCACCTACCAA+CGG 0.675324 2.2:-30526085 MS.gene069431:CDS
AGGGAACGTAGCAACGAGAG+TGG 0.679886 2.2:+30525997 None:intergenic
ACTGGTTGGAGTGTTCCAAG+TGG 0.717431 2.2:-30527705 MS.gene069431:CDS
CCTACTCCACCTACCAACGG+AGG 0.737055 2.2:-30526082 MS.gene069431:CDS
AGCAATATTAGAAAGCACAG+AGG 0.775343 2.2:-30527754 MS.gene069431:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTATTATTATTATTATT+GGG + chr2.2:30526325-30526344 None:intergenic 0.0%
!! TAATTTATTATTATTATTAT+TGG + chr2.2:30526326-30526345 None:intergenic 0.0%
!! ATTGAATATTGAATATTTAA+GGG - chr2.2:30526792-30526811 MS.gene069431:intron 10.0%
!! TATTGAATATTGAATATTTA+AGG - chr2.2:30526791-30526810 MS.gene069431:intron 10.0%
!! TTCAAAAAATAATCTAAATT+TGG + chr2.2:30526382-30526401 None:intergenic 10.0%
!! TTTGTTTACTATAAAATATA+TGG + chr2.2:30526464-30526483 None:intergenic 10.0%
!!! ATTACTTTTTTTTTTTTTTG+AGG + chr2.2:30527140-30527159 None:intergenic 10.0%
!! TCTGTTAATAATTAAATCAA+TGG - chr2.2:30526554-30526573 MS.gene069431:intron 15.0%
!!! ATTTTTTTAAAGTTGTCTAA+TGG + chr2.2:30526937-30526956 None:intergenic 15.0%
!!! TTTGTTATTCTATAATAAGT+TGG + chr2.2:30526735-30526754 None:intergenic 15.0%
!! AAAATGGGACTATTAAAATA+AGG + chr2.2:30527374-30527393 None:intergenic 20.0%
!! AATTGCAACATTAATCAAAA+TGG - chr2.2:30527473-30527492 MS.gene069431:intron 20.0%
!! TATTATAGAATAACAAACCT+AGG - chr2.2:30526738-30526757 MS.gene069431:intron 20.0%
!! TGAATAAACACTTAATAAAG+AGG + chr2.2:30526989-30527008 None:intergenic 20.0%
!! TTCAATATTCAATATTCCAA+CGG + chr2.2:30526785-30526804 None:intergenic 20.0%
!!! GAATCTTATCTTTTGTATTT+GGG - chr2.2:30526246-30526265 MS.gene069431:CDS 20.0%
!!! GTTTTGTTTGAATGTATTAT+TGG + chr2.2:30526115-30526134 None:intergenic 20.0%
!!! GTTTTTTTTTTTCAAGTTAC+CGG - chr2.2:30527093-30527112 MS.gene069431:intron 20.0%
!!! TAGATTATTTTTTGAACAGA+TGG - chr2.2:30526386-30526405 MS.gene069431:intron 20.0%
!!! TATTTTATATGTACCATTCA+AGG - chr2.2:30527551-30527570 MS.gene069431:intron 20.0%
!!! TCTTTTACATCTTGAAAATA+GGG + chr2.2:30527325-30527344 None:intergenic 20.0%
!!! TGAATCTTATCTTTTGTATT+TGG - chr2.2:30526245-30526264 MS.gene069431:CDS 20.0%
! AATTGAAAACTGAAATTGGA+CGG + chr2.2:30527406-30527425 None:intergenic 25.0%
! CAACTATCAAGATCTAATAT+GGG + chr2.2:30526532-30526551 None:intergenic 25.0%
! CTATTGTTAGAATAAGCAAT+TGG + chr2.2:30527190-30527209 None:intergenic 25.0%
! GCAAAATTGAAAACTGAAAT+TGG + chr2.2:30527410-30527429 None:intergenic 25.0%
! TCAACTATCAAGATCTAATA+TGG + chr2.2:30526533-30526552 None:intergenic 25.0%
! TTGTTTGAATGTATTATTGG+CGG + chr2.2:30526112-30526131 None:intergenic 25.0%
!! ATTTCAGTTTTCAATTTTGC+CGG - chr2.2:30527410-30527429 MS.gene069431:intron 25.0%
!! CCATTTTCCATTTAGAAAAT+GGG - chr2.2:30526865-30526884 MS.gene069431:intron 25.0%
!! CTCTTTTACATCTTGAAAAT+AGG + chr2.2:30527326-30527345 None:intergenic 25.0%
!! TCCATTTTCCATTTAGAAAA+TGG - chr2.2:30526864-30526883 MS.gene069431:intron 25.0%
AAGTAATCTAACGTGTGATT+TGG - chr2.2:30527153-30527172 MS.gene069431:intron 30.0%
ATAGAATAACAAACCTAGGT+TGG - chr2.2:30526742-30526761 MS.gene069431:intron 30.0%
CAGTATCGTGAACTTAAAAT+TGG + chr2.2:30526598-30526617 None:intergenic 30.0%
TTTACATCTTGAAAATAGGG+AGG + chr2.2:30527322-30527341 None:intergenic 30.0%
TTTCTAAATGGAAAATGGAG+AGG + chr2.2:30526863-30526882 None:intergenic 30.0%
! ATTCAGTTGCAAGTTTGTTT+AGG + chr2.2:30526230-30526249 None:intergenic 30.0%
! CCCATTTTCTAAATGGAAAA+TGG + chr2.2:30526868-30526887 None:intergenic 30.0%
! TGGAATTCCCATTTTCTAAA+TGG + chr2.2:30526875-30526894 None:intergenic 30.0%
! TTTTGAACAGATGGTTGTAA+CGG - chr2.2:30526395-30526414 MS.gene069431:intron 30.0%
!! AAACTTTAACGCTTAGCTTT+TGG + chr2.2:30527592-30527611 None:intergenic 30.0%
!! TCTGAAATGTTGGTTTATAG+TGG - chr2.2:30526823-30526842 MS.gene069431:intron 30.0%
ACGCTATAAGTATGAAGAAG+TGG + chr2.2:30526904-30526923 None:intergenic 35.0%
AGAGTTACTCTTAACAGAAC+TGG - chr2.2:30527521-30527540 MS.gene069431:intron 35.0%
CTATAAGTATGAAGAAGTGG+AGG + chr2.2:30526901-30526920 None:intergenic 35.0%
GGACGGCACAATAAAAAAAT+GGG + chr2.2:30527389-30527408 None:intergenic 35.0%
TGGACGGCACAATAAAAAAA+TGG + chr2.2:30527390-30527409 None:intergenic 35.0%
TTCAAACAAAACGATGTCCT+AGG - chr2.2:30526123-30526142 MS.gene069431:CDS 35.0%
! AGCAATATTAGAAAGCACAG+AGG - chr2.2:30526011-30526030 MS.gene069431:CDS 35.0%
!! AAATGTTGGTTTATAGTGGC+AGG - chr2.2:30526827-30526846 MS.gene069431:intron 35.0%
!! AATGTTGGTTTATAGTGGCA+GGG - chr2.2:30526828-30526847 MS.gene069431:intron 35.0%
!! GGTAACAAACTCTGAAATGT+TGG - chr2.2:30526813-30526832 MS.gene069431:intron 35.0%
!!! ATTTTCAGTTTGCTGTTCCA+CGG - chr2.2:30526296-30526315 MS.gene069431:CDS 35.0%
AATGGCCAAGTCATAACAAC+AGG - chr2.2:30527491-30527510 MS.gene069431:intron 40.0%
ACACACGTTAGATTACATGC+CGG + chr2.2:30527115-30527134 None:intergenic 40.0%
ACCATTCAAGGTCATTGTTC+CGG - chr2.2:30527563-30527582 MS.gene069431:intron 40.0%
ATGATAACCGCAATGAGAAC+AGG + chr2.2:30527791-30527810 None:intergenic 40.0%
CTGTTAAGAGTAACTCTTGC+TGG + chr2.2:30527518-30527537 None:intergenic 40.0%
GCATATACACAAACTCACCT+AGG + chr2.2:30526143-30526162 None:intergenic 40.0%
GTATGAAGAAGTGGAGGATA+TGG + chr2.2:30526895-30526914 None:intergenic 40.0%
TGATAACCGCAATGAGAACA+GGG + chr2.2:30527790-30527809 None:intergenic 40.0%
TTATTATTATTGGGCCACCG+TGG + chr2.2:30526316-30526335 None:intergenic 40.0%
TTGTTGAACTAGAGCAGAGT+TGG + chr2.2:30527040-30527059 None:intergenic 40.0%
! CGCTTCGTTTTACTCAGATT+GGG - chr2.2:30526086-30526105 MS.gene069431:CDS 40.0%
! TCAGTTTTCAATTTTGCCGG+AGG - chr2.2:30527413-30527432 MS.gene069431:intron 40.0%
ACCGGAACAATGACCTTGAA+TGG + chr2.2:30527567-30527586 None:intergenic 45.0%
ATTCAAGGTCATTGTTCCGG+TGG - chr2.2:30527566-30527585 MS.gene069431:intron 45.0%
CACTGTTGCTGAAGTAAGCA+AGG - chr2.2:30527439-30527458 MS.gene069431:intron 45.0%
CTGGTCCTGTTGTTATGACT+TGG + chr2.2:30527499-30527518 None:intergenic 45.0%
GTAGGAGTAGTTCCTGATGT+AGG + chr2.2:30527668-30527687 None:intergenic 45.0%
TACGTTCCCTGTTCTCATTG+CGG - chr2.2:30527781-30527800 MS.gene069431:CDS 45.0%
TGCTTACTTCAGCAACAGTG+TGG + chr2.2:30527439-30527458 None:intergenic 45.0%
! GCGCTTCGTTTTACTCAGAT+TGG - chr2.2:30526085-30526104 MS.gene069431:CDS 45.0%
!! AATAATAATAAATTAATGAT+AGG - chr2.2:30526331-30526350 MS.gene069431:CDS 5.0%
ACCAACTGAGCTACCAACCT+AGG + chr2.2:30526758-30526777 None:intergenic 50.0%
ACTCCTACTCCACCTACCAA+CGG - chr2.2:30527680-30527699 MS.gene069431:CDS 50.0%
ACTTGGAACACTCCAACCAG+TGG + chr2.2:30526061-30526080 None:intergenic 50.0%
GAGGCAGCAGATCATACTGT+TGG - chr2.2:30526030-30526049 MS.gene069431:CDS 50.0%
GATGTAGGAGGAGTAGGAGA+AGG + chr2.2:30527635-30527654 None:intergenic 50.0%
GCAGCAGATCATACTGTTGG+AGG - chr2.2:30526033-30526052 MS.gene069431:CDS 50.0%
GGAGTAGATGTAGGAGGAGT+AGG + chr2.2:30527641-30527660 None:intergenic 50.0%
GGAGTAGTTCCTGATGTAGG+AGG + chr2.2:30527665-30527684 None:intergenic 50.0%
GTAGGAGGAGGAGTAGATGT+AGG + chr2.2:30527650-30527669 None:intergenic 50.0%
GTAGTTCCTGATGTAGGAGG+AGG + chr2.2:30527662-30527681 None:intergenic 50.0%
TCTACTCCTCCTCCTACATC+AGG - chr2.2:30527653-30527672 MS.gene069431:CDS 50.0%
! ACTGGTTGGAGTGTTCCAAG+TGG - chr2.2:30526060-30526079 MS.gene069431:CDS 50.0%
! GTGATGGAGGAGTTGCATCA+GGG + chr2.2:30527736-30527755 None:intergenic 50.0%
!! AACGCTTAGCTTTTGGCCAC+CGG + chr2.2:30527585-30527604 None:intergenic 50.0%
!! ACCTAGGTTGGTAGCTCAGT+TGG - chr2.2:30526754-30526773 MS.gene069431:intron 50.0%
!! AGCTCAGTTGGTAACTCCGT+TGG - chr2.2:30526766-30526785 MS.gene069431:intron 50.0%
!! TTCAGTTTGCTGTTCCACGG+TGG - chr2.2:30526299-30526318 MS.gene069431:CDS 50.0%
AGGAGAAGGAGGAGAAGTCG+AGG + chr2.2:30527621-30527640 None:intergenic 55.0%
AGGGAACGTAGCAACGAGAG+TGG + chr2.2:30527771-30527790 None:intergenic 55.0%
GATGCAACTCCTCCATCACC+AGG - chr2.2:30527737-30527756 MS.gene069431:CDS 55.0%
GGAGGAGGAGTAGATGTAGG+AGG + chr2.2:30527647-30527666 None:intergenic 55.0%
GTAGGAGGAGTAGGAGAAGG+AGG + chr2.2:30527632-30527651 None:intergenic 55.0%
TTCAGCAACAGTGTGGCCTC+CGG + chr2.2:30527432-30527451 None:intergenic 55.0%
! GGTTGGAGTGTTCCAAGTGG+CGG - chr2.2:30526063-30526082 MS.gene069431:CDS 55.0%
!! CATACTGTTGGAGGCACCAC+TGG - chr2.2:30526042-30526061 MS.gene069431:CDS 55.0%
!! GAAGGTGTTCCTCCGTTGGT+AGG + chr2.2:30527695-30527714 None:intergenic 55.0%
!! GGTGATGGAGGAGTTGCATC+AGG + chr2.2:30527737-30527756 None:intergenic 55.0%
!! TAAAACGAAGCGCCGCCACT+TGG + chr2.2:30526078-30526097 None:intergenic 55.0%
ACGAGAGTGGTAGCTGAGCC+TGG + chr2.2:30527758-30527777 None:intergenic 60.0%
CCTACTCCACCTACCAACGG+AGG - chr2.2:30527683-30527702 MS.gene069431:CDS 60.0%
GTGGTAGCTGAGCCTGGTGA+TGG + chr2.2:30527752-30527771 None:intergenic 60.0%
TGGAGGAGTTGCATCAGGGC+CGG + chr2.2:30527732-30527751 None:intergenic 60.0%
! CCTCCGTTGGTAGGTGGAGT+AGG + chr2.2:30527686-30527705 None:intergenic 60.0%
! GGTGTTCCTCCGTTGGTAGG+TGG + chr2.2:30527692-30527711 None:intergenic 60.0%
!! CTGTTGGAGGCACCACTGGT+TGG - chr2.2:30526046-30526065 MS.gene069431:CDS 60.0%
!! GTAGCTGAGCCTGGTGATGG+AGG + chr2.2:30527749-30527768 None:intergenic 60.0%
AGGAGTTGCATCAGGGCCGG+TGG + chr2.2:30527729-30527748 None:intergenic 65.0%
CCTTCGCCGTCATCTCCCAC+CGG - chr2.2:30527710-30527729 MS.gene069431:CDS 65.0%
GGAGTTGCATCAGGGCCGGT+GGG + chr2.2:30527728-30527747 None:intergenic 65.0%
TCAGGGCCGGTGGGAGATGA+CGG + chr2.2:30527719-30527738 None:intergenic 65.0%
!! CGGCGAAGGTGTTCCTCCGT+TGG + chr2.2:30527699-30527718 None:intergenic 65.0%
! CCGGTGGGAGATGACGGCGA+AGG + chr2.2:30527713-30527732 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 30525961 30527826 30525961 ID=MS.gene069431
chr2.2 mRNA 30525961 30527826 30525961 ID=MS.gene069431.t1;Parent=MS.gene069431
chr2.2 exon 30527643 30527826 30527643 ID=MS.gene069431.t1.exon1;Parent=MS.gene069431.t1
chr2.2 CDS 30527643 30527826 30527643 ID=cds.MS.gene069431.t1;Parent=MS.gene069431.t1
chr2.2 exon 30525961 30526370 30525961 ID=MS.gene069431.t1.exon2;Parent=MS.gene069431.t1
chr2.2 CDS 30525961 30526370 30525961 ID=cds.MS.gene069431.t1;Parent=MS.gene069431.t1
Gene Sequence

>MS.gene069431

ATGTCTCAGTTAAGGAATATGTCAATTCTCTTTGTAGTTGCTTTTGCTGCAGCAATATTAGAAAGCACAGAGGCAGCAGATCATACTGTTGGAGGCACCACTGGTTGGAGTGTTCCAAGTGGCGGCGCTTCGTTTTACTCAGATTGGGCCGCCAATAATACATTCAAACAAAACGATGTCCTAGGTGAGTTTGTGTATATGCTTTTAAAATTTTAATTGACTCTCAAATTTGATTCAAATATTTTTGAACATTACTATTACATTCTCCTAAACAAACTTGCAACTGAATCTTATCTTTTGTATTTGGGAATTTTCAATACTTCTCATACACAGAGATTTTCAGTTTGCTGTTCCACGGTGGCCCAATAATAATAATAATAAATTAATGATAGGCTTTTACACTGTCTCACATTTTGCACCAAATTTAGATTATTTTTTGAACAGATGGTTGTAACGGTGAACTTAAAAGATATCATCATCTTAGTGAACTTCAAAAGCAACCATATATTTTATAGTAAACAAAATTGATCTAATTTGTCTTTTGTCAATGTATTTGACTAATAGCGAACCCATATTAGATCTTGATAGTTGAATCTGTTAATAATTAAATCAATGGTTATTGTCTAACTTCACTCCAATTTTAAGTTCACGATACTGAGTTACTGACTGTCAAACATTTTTTTGTAAAAGAGAATAGAATTTCTCATAAGTTTTTTTCTTCTTCTGTATTATAATGAACTTTGTACAATATTTTATAAAATCATTCATTTTCCAACTTATTATAGAATAACAAACCTAGGTTGGTAGCTCAGTTGGTAACTCCGTTGGAATATTGAATATTGAATATTTAAGGGTAACAAACTCTGAAATGTTGGTTTATAGTGGCAGGGAGTTAGAATCCTCTCCATTTTCCATTTAGAAAATGGGAATTCCATATCCTCCACTTCTTCATACTTATAGCGTGTGAGTTTAGCCATTAGACAACTTTAAAAAAATAAAAAATCTTGAAATTATTCAACACGTTACCTCTTTATTAAGTGTTTATTCAATGATGAGTGATTGATTAAACTGTTTAACCAACTCTGCTCTAGTTCAACAAAATCAGTTTATAAAATAGCAATGAGATATAAGTGTTTTTTTTTTTCAAGTTACCGGCATGTAATCTAACGTGTGTTTCCTCAAAAAAAAAAAAAAGTAATCTAACGTGTGATTTGGTTTTTTACTTACCAATTGCTTATTCTAACAATAGTAATATTTATACACATTTATTTATCTATATACATTCATACAACACATTATTCTTTTTTTTATATCTCTCTTTTTCAATCACTTATAACACATCACAATTATCACATCAACCTCCCTATTTTCAAGATGTAAAAGAGATATTGAGTGTCTATAAAATATTTTCCTTATTTTAATAGTCCCATTTTTTTATTGTGCCGTCCAATTTCAGTTTTCAATTTTGCCGGAGGCCACACTGTTGCTGAAGTAAGCAAGGCAGATTTTGACAATTGCAACATTAATCAAAATGGCCAAGTCATAACAACAGGACCAGCAAGAGTTACTCTTAACAGAACTGGTGATTTCTATTTTATATGTACCATTCAAGGTCATTGTTCCGGTGGCCAAAAGCTAAGCGTTAAAGTTTCAGCTTCCTCGACTTCTCCTCCTTCTCCTACTCCTCCTACATCTACTCCTCCTCCTACATCAGGAACTACTCCTACTCCACCTACCAACGGAGGAACACCTTCGCCGTCATCTCCCACCGGCCCTGATGCAACTCCTCCATCACCAGGCTCAGCTACCACTCTCGTTGCTACGTTCCCTGTTCTCATTGCGGTTATCATGAACATATTGTTCTAG
Protein sequence

>MS.gene069431.t1

MSQLRNMSILFVVAFAAAILESTEAADHTVGGTTGWSVPSGGASFYSDWAANNTFKQNDVLVFNFAGGHTVAEVSKADFDNCNINQNGQVITTGPARVTLNRTGDFYFICTIQGHCSGGQKLSVKVSASSTSPPSPTPPTSTPPPTSGTTPTPPTNGGTPSPSSPTGPDATPPSPGSATTLVATFPVLIAVIMNILF