AlfalfaGEDB | MS.gene069568

M. sativa cultivar XinJiangDaYe / MS.gene069568

KEGG: K07052
GO: GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0016020 GO:0016021 GO:0016485 GO:0016787 GO:0019538 GO:0030176 GO:0031224 GO:0031227 GO:0031300 GO:0031301 GO:0043170 GO:0044238 GO:0051604 GO:0070011 GO:0071586 GO:0071704 GO:0110165 GO:0140096 GO:1901564
PFAM: PF02517.19

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069568.t1	RHN43423.1	99.3	153	1	0	10	162	136	288	6.20E-81	310.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069568.t1	A0A072TUG0	99.3	153	1	0	10	162	135	287	4.5e-81	310.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049865	MS.gene069568	0.812365	4.40E-51	-1.69E-46
MS.gene050170	MS.gene069568	0.86006	2.69E-63	-1.69E-46
MS.gene050323	MS.gene069568	0.82428	8.79E-54	-1.69E-46
MS.gene050496	MS.gene069568	0.827641	1.40E-54	-1.69E-46
MS.gene050577	MS.gene069568	0.825194	5.35E-54	-1.69E-46
MS.gene050697	MS.gene069568	0.816291	5.97E-52	-1.69E-46
MS.gene050868	MS.gene069568	0.811624	6.38E-51	-1.69E-46
MS.gene050871	MS.gene069568	0.80738	5.20E-50	-1.69E-46
MS.gene050904	MS.gene069568	0.825388	4.82E-54	-1.69E-46
MS.gene052482	MS.gene069568	0.808992	2.36E-50	-1.69E-46
MS.gene052486	MS.gene069568	0.813673	2.27E-51	-1.69E-46
MS.gene053083	MS.gene069568	-0.835444	1.67E-56	-1.69E-46
MS.gene053097	MS.gene069568	-0.80033	1.52E-48	-1.69E-46
MS.gene05470	MS.gene069568	0.806809	6.87E-50	-1.69E-46
MS.gene055251	MS.gene069568	-0.805786	1.13E-49	-1.69E-46
MS.gene055750	MS.gene069568	0.815293	9.96E-52	-1.69E-46
MS.gene055751	MS.gene069568	0.80242	5.66E-49	-1.69E-46
MS.gene05589	MS.gene069568	0.83737	5.41E-57	-1.69E-46
MS.gene056266	MS.gene069568	0.858893	6.03E-63	-1.69E-46
MS.gene056504	MS.gene069568	0.817168	3.79E-52	-1.69E-46
MS.gene058334	MS.gene069568	-0.810438	1.15E-50	-1.69E-46
MS.gene058382	MS.gene069568	-0.825966	3.51E-54	-1.69E-46
MS.gene059023	MS.gene069568	0.807343	5.29E-50	-1.69E-46
MS.gene059837	MS.gene069568	0.847518	1.10E-59	-1.69E-46
MS.gene05996	MS.gene069568	0.828291	9.76E-55	-1.69E-46
MS.gene06000	MS.gene069568	0.836359	9.80E-57	-1.69E-46
MS.gene06027	MS.gene069568	0.879805	9.23E-70	-1.69E-46
MS.gene06042	MS.gene069568	-0.811588	6.49E-51	-1.69E-46
MS.gene060595	MS.gene069568	-0.823124	1.64E-53	-1.69E-46
MS.gene06077	MS.gene069568	0.810865	9.32E-51	-1.69E-46
MS.gene060982	MS.gene069568	0.836709	7.98E-57	-1.69E-46
MS.gene061044	MS.gene069568	-0.838948	2.12E-57	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069568.t1	MTR_8g099085	99.346	153	1	0	10	162	135	287	1.42e-106	308
MS.gene069568.t1	MTR_1g099420	67.500	160	52	0	3	162	127	286	2.90e-77	233

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069568.t1	AT5G60750	75.974	154	36	1	10	162	138	291	1.26e-78	237

Find 51 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATCAGTGACAAGGCTAAAT+AGG	0.223638	4.2:-5254143	None:intergenic
ACACCACACAGGCATGTATT+TGG	0.285573	4.2:-5254499	None:intergenic
AAGGATTCTTTGACATTTAG+AGG	0.293855	4.2:+5254114	MS.gene069568:CDS
CCTTGCTGAAGCCAGTTATA+TGG	0.298053	4.2:-5254095	None:intergenic
AGTTTAGCCTGGAAGGAAAA+TGG	0.299581	4.2:+5254244	MS.gene069568:CDS
GCCTGTGTGGTGTGCAATAC+TGG	0.349411	4.2:+5254509	MS.gene069568:CDS
TCATGTTTCCTCTTGTCAAT+CGG	0.417015	4.2:+5254292	MS.gene069568:CDS
ATCAGTGACAAGGCTAAATA+GGG	0.430586	4.2:-5254142	None:intergenic
TCCGTCCCCTTCCACCCGAC+TGG	0.444389	4.2:+5254217	MS.gene069568:CDS
CTTGCTGAAGCCAGTTATAT+GGG	0.459032	4.2:-5254094	None:intergenic
TTCTTTGACATTTAGAGGGC+AGG	0.461094	4.2:+5254119	MS.gene069568:CDS
TCGATGGCAGGATGGGTAGT+AGG	0.477125	4.2:-5254335	None:intergenic
TGATTGTTCAACACTTGAAA+GGG	0.482634	4.2:-5254367	None:intergenic
TTGATTGTTCAACACTTGAA+AGG	0.487389	4.2:-5254368	None:intergenic
CGACTGGTTCAAGTTTAGCC+TGG	0.494889	4.2:+5254233	MS.gene069568:CDS
ATCGGTCTGCGCTCCTGTGT+GGG	0.497992	4.2:+5254437	MS.gene069568:CDS
CTTGAACCAGTCGGGTGGAA+GGG	0.500064	4.2:-5254223	None:intergenic
ACCTGTGTCGATGGCAGGAT+GGG	0.500774	4.2:-5254342	None:intergenic
TTGAACCAGTCGGGTGGAAG+GGG	0.510746	4.2:-5254222	None:intergenic
CCAGTTATATGGGCTGCAAA+AGG	0.512015	4.2:-5254084	None:intergenic
TATCGGTCTGCGCTCCTGTG+TGG	0.516259	4.2:+5254436	MS.gene069568:CDS
GACCTGTGTCGATGGCAGGA+TGG	0.530416	4.2:-5254343	None:intergenic
GATGTAACTGAAGGACTAGC+TGG	0.531776	4.2:+5254162	MS.gene069568:CDS
ACTTGAACCAGTCGGGTGGA+AGG	0.533549	4.2:-5254224	None:intergenic
AGGCTAAACTTGAACCAGTC+GGG	0.535684	4.2:-5254231	None:intergenic
GTTGTATGCAATTGTAGTAT+CGG	0.544728	4.2:+5254419	MS.gene069568:CDS
GGGTGACCTGTGTCGATGGC+AGG	0.545730	4.2:-5254347	None:intergenic
AATATGGTCGTTGCACTCCA+TGG	0.550589	4.2:-5253819	None:intergenic
TGGTTCAAGTTTAGCCTGGA+AGG	0.555909	4.2:+5254237	MS.gene069568:CDS
TTGAACAATCAATAAAAGCA+AGG	0.561023	4.2:+5254379	MS.gene069568:CDS
GCATGTATTTGGTAAGTGAC+GGG	0.563519	4.2:-5254488	None:intergenic
ACCAGTCGGGTGGAAGGGGA+CGG	0.564379	4.2:-5254218	None:intergenic
GGCATGTATTTGGTAAGTGA+CGG	0.567093	4.2:-5254489	None:intergenic
CAGGCTAAACTTGAACCAGT+CGG	0.579922	4.2:-5254232	None:intergenic
AGGATTCTTTGACATTTAGA+GGG	0.596115	4.2:+5254115	MS.gene069568:CDS
TGAGAGAGCCGATTGACAAG+AGG	0.610131	4.2:-5254300	None:intergenic
GCAGGATGGGTAGTAGGTCA+AGG	0.610496	4.2:-5254329	None:intergenic
GCATACAACAACATTGCCAC+AGG	0.610600	4.2:-5254405	None:intergenic
TGAACAATCAATAAAAGCAA+GGG	0.613230	4.2:+5254380	MS.gene069568:CDS
GGTCTGCGCTCCTGTGTGGG+AGG	0.621158	4.2:+5254440	MS.gene069568:CDS
CCATATAACTGGCTTCAGCA+AGG	0.626497	4.2:+5254095	MS.gene069568:CDS
CTTGTCACTGATGTAACTGA+AGG	0.627885	4.2:+5254153	MS.gene069568:CDS
ACCCATCCTGCCATCGACAC+AGG	0.628035	4.2:+5254341	MS.gene069568:CDS
ACCAGTATTGCACACCACAC+AGG	0.628546	4.2:-5254510	None:intergenic
CTAAACTTGAACCAGTCGGG+TGG	0.632925	4.2:-5254228	None:intergenic
AATAAAAGCAAGGGATCCTG+TGG	0.652461	4.2:+5254389	MS.gene069568:CDS
GAAAGGGTGACCTGTGTCGA+TGG	0.653532	4.2:-5254351	None:intergenic
CTTCAGTTACATCAGTGACA+AGG	0.657659	4.2:-5254152	None:intergenic
AAAACTATTTCCTCCCACAC+AGG	0.659992	4.2:-5254450	None:intergenic
TTACCAAATACATGCCTGTG+TGG	0.698661	4.2:+5254496	MS.gene069568:CDS
GTGACGGGAGTAGAAAACCG+CGG	0.770907	4.2:-5254473	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AGAAACACTTACTTTTAATA+TGG	-	chr4.2:5253838-5253857	None:intergenic	20.0%
!	TGAACAATCAATAAAAGCAA+GGG	+	chr4.2:5254380-5254399	MS.gene069568:CDS	25.0%
!	TTGAACAATCAATAAAAGCA+AGG	+	chr4.2:5254379-5254398	MS.gene069568:CDS	25.0%
	AAGGATTCTTTGACATTTAG+AGG	+	chr4.2:5254114-5254133	MS.gene069568:CDS	30.0%
	AGGATTCTTTGACATTTAGA+GGG	+	chr4.2:5254115-5254134	MS.gene069568:CDS	30.0%
	ATTCCTCGCAGTTAAAATTT+AGG	+	chr4.2:5253908-5253927	MS.gene069568:intron	30.0%
	GTTGTATGCAATTGTAGTAT+CGG	+	chr4.2:5254419-5254438	MS.gene069568:CDS	30.0%
	TGATTGTTCAACACTTGAAA+GGG	-	chr4.2:5254370-5254389	None:intergenic	30.0%
	TTCCTCGCAGTTAAAATTTA+GGG	+	chr4.2:5253909-5253928	MS.gene069568:intron	30.0%
	TTGATTGTTCAACACTTGAA+AGG	-	chr4.2:5254371-5254390	None:intergenic	30.0%
!	GTTAAAATTTAGGGTACATG+TGG	+	chr4.2:5253918-5253937	MS.gene069568:intron	30.0%
!!!	CATTTTGGTTTATAGGATCT+TGG	+	chr4.2:5254055-5254074	MS.gene069568:intron	30.0%
	ATCAGTGACAAGGCTAAATA+GGG	-	chr4.2:5254145-5254164	None:intergenic	35.0%
	GATTTCAACTCTGCTCAATT+GGG	-	chr4.2:5253960-5253979	None:intergenic	35.0%
	TCATGTTTCCTCTTGTCAAT+CGG	+	chr4.2:5254292-5254311	MS.gene069568:CDS	35.0%
!!	ATGGCATTTTGATGTTGTCA+TGG	+	chr4.2:5254263-5254282	MS.gene069568:CDS	35.0%
!!	TGGCATTTTGATGTTGTCAT+GGG	+	chr4.2:5254264-5254283	MS.gene069568:CDS	35.0%
!!!	ACATCGGCATTTTGGTTTAT+AGG	+	chr4.2:5254048-5254067	MS.gene069568:intron	35.0%
!!!	TACCCTAAATTTTAACTGCG+AGG	-	chr4.2:5253914-5253933	None:intergenic	35.0%
	AAAACTATTTCCTCCCACAC+AGG	-	chr4.2:5254453-5254472	None:intergenic	40.0%
	AACAATAAACGCTCAACCTG+AGG	+	chr4.2:5253983-5254002	MS.gene069568:intron	40.0%
	AATAAAAGCAAGGGATCCTG+TGG	+	chr4.2:5254389-5254408	MS.gene069568:CDS	40.0%
	AGGTTGAGCGTTTATTGTTC+AGG	-	chr4.2:5253982-5254001	None:intergenic	40.0%
	AGTTTAGCCTGGAAGGAAAA+TGG	+	chr4.2:5254244-5254263	MS.gene069568:CDS	40.0%
	CATCAGTGACAAGGCTAAAT+AGG	-	chr4.2:5254146-5254165	None:intergenic	40.0%
	CTTCAGTTACATCAGTGACA+AGG	-	chr4.2:5254155-5254174	None:intergenic	40.0%
	CTTGCTGAAGCCAGTTATAT+GGG	-	chr4.2:5254097-5254116	None:intergenic	40.0%
	CTTGTCACTGATGTAACTGA+AGG	+	chr4.2:5254153-5254172	MS.gene069568:CDS	40.0%
	GCATGTATTTGGTAAGTGAC+GGG	-	chr4.2:5254491-5254510	None:intergenic	40.0%
	GGATTTCAACTCTGCTCAAT+TGG	-	chr4.2:5253961-5253980	None:intergenic	40.0%
	GGCATGTATTTGGTAAGTGA+CGG	-	chr4.2:5254492-5254511	None:intergenic	40.0%
	GGTTATGCTTCTTTGGACAT+CGG	+	chr4.2:5254032-5254051	MS.gene069568:intron	40.0%
	TTACCAAATACATGCCTGTG+TGG	+	chr4.2:5254496-5254515	MS.gene069568:CDS	40.0%
	TTCTTTGACATTTAGAGGGC+AGG	+	chr4.2:5254119-5254138	MS.gene069568:CDS	40.0%
!	TTCTTTGGACATCGGCATTT+TGG	+	chr4.2:5254040-5254059	MS.gene069568:intron	40.0%
!!!	CAAAATGCCATTTTCCTTCC+AGG	-	chr4.2:5254254-5254273	None:intergenic	40.0%
	ACACCACACAGGCATGTATT+TGG	-	chr4.2:5254502-5254521	None:intergenic	45.0%
	AGGCTAAACTTGAACCAGTC+GGG	-	chr4.2:5254234-5254253	None:intergenic	45.0%
	ATTCGTGCAATCTTTGACGC+AGG	+	chr4.2:5254011-5254030	MS.gene069568:intron	45.0%
	ATTGCACGAATTACAGCCTC+AGG	-	chr4.2:5254002-5254021	None:intergenic	45.0%
	CAGGCTAAACTTGAACCAGT+CGG	-	chr4.2:5254235-5254254	None:intergenic	45.0%
	CCTTGCTGAAGCCAGTTATA+TGG	-	chr4.2:5254098-5254117	None:intergenic	45.0%
	GCATACAACAACATTGCCAC+AGG	-	chr4.2:5254408-5254427	None:intergenic	45.0%
	TAGGGTACATGTGGCAATCA+TGG	+	chr4.2:5253927-5253946	MS.gene069568:intron	45.0%
	TGACGCAGGTTATGCTTCTT+TGG	+	chr4.2:5254025-5254044	MS.gene069568:intron	45.0%
!	CCAGTTATATGGGCTGCAAA+AGG	-	chr4.2:5254087-5254106	None:intergenic	45.0%
!	CCATATAACTGGCTTCAGCA+AGG	+	chr4.2:5254095-5254114	MS.gene069568:CDS	45.0%
!	CCTTTTGCAGCCCATATAAC+TGG	+	chr4.2:5254084-5254103	MS.gene069568:CDS	45.0%
!	GATGTAACTGAAGGACTAGC+TGG	+	chr4.2:5254162-5254181	MS.gene069568:CDS	45.0%
!	TGGGAGGAAATAGTTTTCCG+CGG	+	chr4.2:5254456-5254475	MS.gene069568:CDS	45.0%
!	TGGTTCAAGTTTAGCCTGGA+AGG	+	chr4.2:5254237-5254256	MS.gene069568:CDS	45.0%
	CTAAACTTGAACCAGTCGGG+TGG	-	chr4.2:5254231-5254250	None:intergenic	50.0%
	TGAGAGAGCCGATTGACAAG+AGG	-	chr4.2:5254303-5254322	None:intergenic	50.0%
!	CGACTGGTTCAAGTTTAGCC+TGG	+	chr4.2:5254233-5254252	MS.gene069568:CDS	50.0%
	ACTTGAACCAGTCGGGTGGA+AGG	-	chr4.2:5254227-5254246	None:intergenic	55.0%
	CTTGAACCAGTCGGGTGGAA+GGG	-	chr4.2:5254226-5254245	None:intergenic	55.0%
	GAAAGGGTGACCTGTGTCGA+TGG	-	chr4.2:5254354-5254373	None:intergenic	55.0%
	GCAGGATGGGTAGTAGGTCA+AGG	-	chr4.2:5254332-5254351	None:intergenic	55.0%
	TCGATGGCAGGATGGGTAGT+AGG	-	chr4.2:5254338-5254357	None:intergenic	55.0%
	TTGAACCAGTCGGGTGGAAG+GGG	-	chr4.2:5254225-5254244	None:intergenic	55.0%
!	ACCTGTGTCGATGGCAGGAT+GGG	-	chr4.2:5254345-5254364	None:intergenic	55.0%
!	GTGACGGGAGTAGAAAACCG+CGG	-	chr4.2:5254476-5254495	None:intergenic	55.0%
	ACCCATCCTGCCATCGACAC+AGG	+	chr4.2:5254341-5254360	MS.gene069568:CDS	60.0%
	ATCGGTCTGCGCTCCTGTGT+GGG	+	chr4.2:5254437-5254456	MS.gene069568:CDS	60.0%
	TATCGGTCTGCGCTCCTGTG+TGG	+	chr4.2:5254436-5254455	MS.gene069568:CDS	60.0%
!	GACCTGTGTCGATGGCAGGA+TGG	-	chr4.2:5254346-5254365	None:intergenic	60.0%
	ACCAGTCGGGTGGAAGGGGA+CGG	-	chr4.2:5254221-5254240	None:intergenic	65.0%
!	GGGTGACCTGTGTCGATGGC+AGG	-	chr4.2:5254350-5254369	None:intergenic	65.0%
	GGTCTGCGCTCCTGTGTGGG+AGG	+	chr4.2:5254440-5254459	MS.gene069568:CDS	70.0%
	TCCGTCCCCTTCCACCCGAC+TGG	+	chr4.2:5254217-5254236	MS.gene069568:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	5253821	5254530	5253821	ID=MS.gene069568
chr4.2	mRNA	5253821	5254530	5253821	ID=MS.gene069568.t1;Parent=MS.gene069568
chr4.2	exon	5253821	5253845	5253821	ID=MS.gene069568.t1.exon1;Parent=MS.gene069568.t1
chr4.2	CDS	5253821	5253845	5253821	ID=cds.MS.gene069568.t1;Parent=MS.gene069568.t1
chr4.2	exon	5254070	5254530	5254070	ID=MS.gene069568.t1.exon2;Parent=MS.gene069568.t1
chr4.2	CDS	5254070	5254530	5254070	ID=cds.MS.gene069568.t1;Parent=MS.gene069568.t1

Gene Sequence

>MS.gene069568

ATGGAGTGCAACGACCATATTAAAAGTAAGTGTTTCTCTAAAGTTTCATCAAAGTATAATTATGAAAAGTTTCGCTTTTGTTTATCAATTCCTCGCAGTTAAAATTTAGGGTACATGTGGCAATCATGGAATGTATCCCAATTGAGCAGAGTTGAAATCCTGAACAATAAACGCTCAACCTGAGGCTGTAATTCGTGCAATCTTTGACGCAGGTTATGCTTCTTTGGACATCGGCATTTTGGTTTATAGGATCTTGGATGATTCCTTTTGCAGCCCATATAACTGGCTTCAGCAAGGATTCTTTGACATTTAGAGGGCAGGCCCTATTTAGCCTTGTCACTGATGTAACTGAAGGACTAGCTGGAATTGCAATTCTTGTTCGTTGTCTTTCACGATTCCGTCCCCTTCCACCCGACTGGTTCAAGTTTAGCCTGGAAGGAAAATGGCATTTTGATGTTGTCATGGGTTGTCTCATGTTTCCTCTTGTCAATCGGCTCTCTCAGTTCAACCTTGACCTACTACCCATCCTGCCATCGACACAGGTCACCCTTTCAAGTGTTGAACAATCAATAAAAGCAAGGGATCCTGTGGCAATGTTGTTGTATGCAATTGTAGTATCGGTCTGCGCTCCTGTGTGGGAGGAAATAGTTTTCCGCGGTTTTCTACTCCCGTCACTTACCAAATACATGCCTGTGTGGTGTGCAATACTG

Protein sequence

>MS.gene069568.t1

MECNDHIKRSWMIPFAAHITGFSKDSLTFRGQALFSLVTDVTEGLAGIAILVRCLSRFRPLPPDWFKFSLEGKWHFDVVMGCLMFPLVNRLSQFNLDLLPILPSTQVTLSSVEQSIKARDPVAMLLYAIVVSVCAPVWEEIVFRGFLLPSLTKYMPVWCAIL