Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071667.t1 | GAU29362.1 | 98.9 | 92 | 1 | 0 | 1 | 92 | 23 | 114 | 2.20E-38 | 167.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071667.t1 | A8IAP8 | 45.6 | 90 | 48 | 1 | 1 | 90 | 31 | 119 | 1.8e-09 | 63.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071667.t1 | A0A2Z6MWZ4 | 98.9 | 92 | 1 | 0 | 1 | 92 | 23 | 114 | 1.6e-38 | 167.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene071667 | -0.802215 | 6.24E-49 | -1.69E-46 |
| MS.gene049419 | MS.gene071667 | 0.860149 | 2.53E-63 | -1.69E-46 |
| MS.gene049801 | MS.gene071667 | -0.805379 | 1.37E-49 | -1.69E-46 |
| MS.gene04991 | MS.gene071667 | 0.806084 | 9.77E-50 | -1.69E-46 |
| MS.gene049983 | MS.gene071667 | -0.801909 | 7.21E-49 | -1.69E-46 |
| MS.gene050105 | MS.gene071667 | -0.843937 | 1.03E-58 | -1.69E-46 |
| MS.gene050361 | MS.gene071667 | -0.805892 | 1.07E-49 | -1.69E-46 |
| MS.gene050654 | MS.gene071667 | -0.827243 | 1.74E-54 | -1.69E-46 |
| MS.gene051122 | MS.gene071667 | -0.847898 | 8.64E-60 | -1.69E-46 |
| MS.gene051275 | MS.gene071667 | -0.802605 | 5.18E-49 | -1.69E-46 |
| MS.gene051368 | MS.gene071667 | -0.817003 | 4.13E-52 | -1.69E-46 |
| MS.gene051793 | MS.gene071667 | 0.812619 | 3.87E-51 | -1.69E-46 |
| MS.gene051828 | MS.gene071667 | 0.804411 | 2.19E-49 | -1.69E-46 |
| MS.gene051924 | MS.gene071667 | 0.817822 | 2.70E-52 | -1.69E-46 |
| MS.gene051926 | MS.gene071667 | 0.826903 | 2.10E-54 | -1.69E-46 |
| MS.gene05216 | MS.gene071667 | -0.861285 | 1.14E-63 | -1.69E-46 |
| MS.gene052674 | MS.gene071667 | -0.884161 | 2.43E-71 | -1.69E-46 |
| MS.gene052675 | MS.gene071667 | -0.888163 | 7.53E-73 | -1.69E-46 |
| MS.gene052677 | MS.gene071667 | -0.891012 | 5.86E-74 | -1.69E-46 |
| MS.gene052965 | MS.gene071667 | 0.802731 | 4.88E-49 | -1.69E-46 |
| MS.gene053726 | MS.gene071667 | -0.814114 | 1.82E-51 | -1.69E-46 |
| MS.gene054479 | MS.gene071667 | -0.86248 | 4.92E-64 | -1.69E-46 |
| MS.gene054950 | MS.gene071667 | 0.80404 | 2.61E-49 | -1.69E-46 |
| MS.gene055063 | MS.gene071667 | 0.807298 | 5.41E-50 | -1.69E-46 |
| MS.gene055327 | MS.gene071667 | -0.808069 | 3.71E-50 | -1.69E-46 |
| MS.gene055701 | MS.gene071667 | -0.848307 | 6.66E-60 | -1.69E-46 |
| MS.gene056045 | MS.gene071667 | -0.809627 | 1.72E-50 | -1.69E-46 |
| MS.gene056064 | MS.gene071667 | -0.849133 | 3.93E-60 | -1.69E-46 |
| MS.gene056480 | MS.gene071667 | -0.856489 | 3.11E-62 | -1.69E-46 |
| MS.gene056779 | MS.gene071667 | -0.822087 | 2.86E-53 | -1.69E-46 |
| MS.gene056873 | MS.gene071667 | -0.821531 | 3.84E-53 | -1.69E-46 |
| MS.gene057156 | MS.gene071667 | 0.869234 | 3.61E-66 | -1.69E-46 |
| MS.gene05761 | MS.gene071667 | -0.802274 | 6.06E-49 | -1.69E-46 |
| MS.gene058081 | MS.gene071667 | -0.843243 | 1.58E-58 | -1.69E-46 |
| MS.gene058223 | MS.gene071667 | 0.889539 | 2.21E-73 | -1.69E-46 |
| MS.gene058429 | MS.gene071667 | -0.837457 | 5.14E-57 | -1.69E-46 |
| MS.gene059438 | MS.gene071667 | -0.817728 | 2.84E-52 | -1.69E-46 |
| MS.gene059440 | MS.gene071667 | -0.820266 | 7.51E-53 | -1.69E-46 |
| MS.gene059952 | MS.gene071667 | -0.827758 | 1.31E-54 | -1.69E-46 |
| MS.gene06052 | MS.gene071667 | -0.830914 | 2.25E-55 | -1.69E-46 |
| MS.gene060777 | MS.gene071667 | -0.80119 | 1.01E-48 | -1.69E-46 |
| MS.gene060850 | MS.gene071667 | -0.872594 | 2.82E-67 | -1.69E-46 |
| MS.gene060851 | MS.gene071667 | -0.888833 | 4.16E-73 | -1.69E-46 |
| MS.gene060852 | MS.gene071667 | -0.883958 | 2.89E-71 | -1.69E-46 |
| MS.gene060932 | MS.gene071667 | -0.835168 | 1.96E-56 | -1.69E-46 |
| MS.gene06121 | MS.gene071667 | -0.826428 | 2.73E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071667.t1 | MTR_1g114200 | 98.913 | 92 | 1 | 0 | 1 | 92 | 23 | 114 | 3.35e-61 | 182 |
| MS.gene071667.t1 | MTR_4g032660 | 33.708 | 89 | 59 | 0 | 1 | 89 | 108 | 196 | 1.27e-12 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071667.t1 | AT5G27820 | 78.261 | 92 | 20 | 0 | 1 | 92 | 23 | 114 | 2.13e-50 | 154 |
| MS.gene071667.t1 | AT3G20230 | 35.955 | 89 | 57 | 0 | 1 | 89 | 95 | 183 | 1.50e-14 | 65.5 |
Find 23 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACATCCCGAGTAGTTTCC+AGG | 0.336475 | 1.1:+80269999 | None:intergenic |
| AAGCGGTTATTGATTCTCTC+AGG | 0.400454 | 1.1:-80269868 | MS.gene071667:CDS |
| GAAAGCCTTGAGATCAAGCC+TGG | 0.444737 | 1.1:-80270017 | MS.gene071667:CDS |
| CTCCTGTGAGCTTGCAGAAG+AGG | 0.447057 | 1.1:+80270039 | None:intergenic |
| ATTGATTCTCTCAGGGAAGC+TGG | 0.452667 | 1.1:-80269860 | MS.gene071667:CDS |
| AAGAGAGAACAGAGGTATCA+TGG | 0.461247 | 1.1:-80269899 | MS.gene071667:CDS |
| GTTGCTGCAGCTGCAAAGAT+TGG | 0.469361 | 1.1:-80269980 | MS.gene071667:CDS |
| AGTTTCCAGGCTTGATCTCA+AGG | 0.483054 | 1.1:+80270012 | None:intergenic |
| TGCCTCTTCTGCAAGCTCAC+AGG | 0.512391 | 1.1:-80270041 | MS.gene071667:CDS |
| GAAGAGGCAACGGTAGCAGT+TGG | 0.531232 | 1.1:+80270055 | None:intergenic |
| GATCAAGCCTGGAAACTACT+CGG | 0.540330 | 1.1:-80270006 | MS.gene071667:CDS |
| AGCGGAACGCCTTTCGCTCA+AGG | 0.547714 | 1.1:-80269948 | MS.gene071667:CDS |
| CAGGAATGTCCTTGAGCGAA+AGG | 0.550217 | 1.1:+80269939 | None:intergenic |
| TTGCTGCAGCTGCAAAGATT+GGG | 0.550997 | 1.1:-80269979 | MS.gene071667:CDS |
| AGCGGTTATTGATTCTCTCA+GGG | 0.559363 | 1.1:-80269867 | MS.gene071667:CDS |
| TTCAAGTGAACAGAAACAGC+AGG | 0.564698 | 1.1:+80269920 | None:intergenic |
| AGAACAGAGGTATCATGGAA+AGG | 0.588862 | 1.1:-80269894 | MS.gene071667:CDS |
| ATCAAGCCTGGAAACTACTC+GGG | 0.593151 | 1.1:-80270005 | MS.gene071667:CDS |
| CAACGGTAGCAGTTGGAGTG+TGG | 0.604975 | 1.1:+80270062 | None:intergenic |
| TGAGCTTGCAGAAGAGGCAA+CGG | 0.638179 | 1.1:+80270045 | None:intergenic |
| GTATCATGGAAAGGTAAAAG+CGG | 0.664247 | 1.1:-80269885 | MS.gene071667:CDS |
| AAAGATTGGGAAGCTACTAG+CGG | 0.716694 | 1.1:-80269966 | MS.gene071667:CDS |
| TTCACTTGAAGAGAGAACAG+AGG | 0.737493 | 1.1:-80269907 | MS.gene071667:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTATCATGGAAAGGTAAAAG+CGG | - | chr1.1:80270059-80270078 | MS.gene071667:CDS | 35.0% | |
| AAAGATTGGGAAGCTACTAG+CGG | - | chr1.1:80269978-80269997 | MS.gene071667:CDS | 40.0% | |
| TTCAAGTGAACAGAAACAGC+AGG | + | chr1.1:80270027-80270046 | None:intergenic | 40.0% | |
| TTCACTTGAAGAGAGAACAG+AGG | - | chr1.1:80270037-80270056 | MS.gene071667:CDS | 40.0% | |
| !! | AAGAGAGAACAGAGGTATCA+TGG | - | chr1.1:80270045-80270064 | MS.gene071667:CDS | 40.0% |
| !! | AAGCGGTTATTGATTCTCTC+AGG | - | chr1.1:80270076-80270095 | MS.gene071667:CDS | 40.0% |
| !! | AGAACAGAGGTATCATGGAA+AGG | - | chr1.1:80270050-80270069 | MS.gene071667:CDS | 40.0% |
| !! | AGCGGTTATTGATTCTCTCA+GGG | - | chr1.1:80270077-80270096 | MS.gene071667:CDS | 40.0% |
| AGTTTCCAGGCTTGATCTCA+AGG | + | chr1.1:80269935-80269954 | None:intergenic | 45.0% | |
| ATCAAGCCTGGAAACTACTC+GGG | - | chr1.1:80269939-80269958 | MS.gene071667:CDS | 45.0% | |
| GATCAAGCCTGGAAACTACT+CGG | - | chr1.1:80269938-80269957 | MS.gene071667:CDS | 45.0% | |
| TTGCTGCAGCTGCAAAGATT+GGG | - | chr1.1:80269965-80269984 | MS.gene071667:CDS | 45.0% | |
| ! | ATTGATTCTCTCAGGGAAGC+TGG | - | chr1.1:80270084-80270103 | MS.gene071667:CDS | 45.0% |
| CAACATCCCGAGTAGTTTCC+AGG | + | chr1.1:80269948-80269967 | None:intergenic | 50.0% | |
| CAGGAATGTCCTTGAGCGAA+AGG | + | chr1.1:80270008-80270027 | None:intergenic | 50.0% | |
| GAAAGCCTTGAGATCAAGCC+TGG | - | chr1.1:80269927-80269946 | MS.gene071667:CDS | 50.0% | |
| GTTGCTGCAGCTGCAAAGAT+TGG | - | chr1.1:80269964-80269983 | MS.gene071667:CDS | 50.0% | |
| TGAGCTTGCAGAAGAGGCAA+CGG | + | chr1.1:80269902-80269921 | None:intergenic | 50.0% | |
| CAACGGTAGCAGTTGGAGTG+TGG | + | chr1.1:80269885-80269904 | None:intergenic | 55.0% | |
| CTCCTGTGAGCTTGCAGAAG+AGG | + | chr1.1:80269908-80269927 | None:intergenic | 55.0% | |
| GAAGAGGCAACGGTAGCAGT+TGG | + | chr1.1:80269892-80269911 | None:intergenic | 55.0% | |
| TGCCTCTTCTGCAAGCTCAC+AGG | - | chr1.1:80269903-80269922 | MS.gene071667:CDS | 55.0% | |
| AGCGGAACGCCTTTCGCTCA+AGG | - | chr1.1:80269996-80270015 | MS.gene071667:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 80269844 | 80270122 | 80269844 | ID=MS.gene071667 |
| chr1.1 | mRNA | 80269844 | 80270122 | 80269844 | ID=MS.gene071667.t1;Parent=MS.gene071667 |
| chr1.1 | exon | 80269844 | 80270122 | 80269844 | ID=MS.gene071667.t1.exon1;Parent=MS.gene071667.t1 |
| chr1.1 | CDS | 80269844 | 80270122 | 80269844 | ID=cds.MS.gene071667.t1;Parent=MS.gene071667.t1 |
| Gene Sequence |
| Protein sequence |
