AlfalfaGEDB | MS.gene073035

M. sativa cultivar XinJiangDaYe / MS.gene073035

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073035.t1	AFK44148.1	89.5	171	18	0	1	171	1	171	5.00E-81	310.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073035.t1	O65695	39.3	89	49	1	49	132	18	106	2.5e-12	73.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073035.t1	G7J2U8	89.5	171	18	0	1	171	1	171	3.6e-81	310.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050170	MS.gene073035	-0.817447	3.28E-52	-1.69E-46
MS.gene050496	MS.gene073035	-0.810191	1.30E-50	-1.69E-46
MS.gene050504	MS.gene073035	-0.819362	1.21E-52	-1.69E-46
MS.gene050612	MS.gene073035	-0.819372	1.20E-52	-1.69E-46
MS.gene050868	MS.gene073035	-0.803297	3.73E-49	-1.69E-46
MS.gene050871	MS.gene073035	-0.801424	9.06E-49	-1.69E-46
MS.gene050883	MS.gene073035	0.811911	5.52E-51	-1.69E-46
MS.gene051127	MS.gene073035	0.829315	5.51E-55	-1.69E-46
MS.gene051138	MS.gene073035	0.836428	9.41E-57	-1.69E-46
MS.gene051364	MS.gene073035	0.801103	1.05E-48	-1.69E-46
MS.gene052606	MS.gene073035	0.81901	1.45E-52	-1.69E-46
MS.gene053420	MS.gene073035	0.819607	1.06E-52	-1.69E-46
MS.gene05470	MS.gene073035	-0.813974	1.95E-51	-1.69E-46
MS.gene055154	MS.gene073035	0.80071	1.27E-48	-1.69E-46
MS.gene055202	MS.gene073035	0.808422	3.12E-50	-1.69E-46
MS.gene055748	MS.gene073035	-0.803311	3.70E-49	-1.69E-46
MS.gene055751	MS.gene073035	-0.814651	1.38E-51	-1.69E-46
MS.gene05589	MS.gene073035	-0.825486	4.57E-54	-1.69E-46
MS.gene056218	MS.gene073035	0.803813	2.91E-49	-1.69E-46
MS.gene056224	MS.gene073035	0.803736	3.02E-49	-1.69E-46
MS.gene056298	MS.gene073035	0.825037	5.83E-54	-1.69E-46
MS.gene056504	MS.gene073035	-0.839854	1.23E-57	-1.69E-46
MS.gene056748	MS.gene073035	0.812044	5.17E-51	-1.69E-46
MS.gene057768	MS.gene073035	0.85196	6.31E-61	-1.69E-46
MS.gene058099	MS.gene073035	0.811014	8.65E-51	-1.69E-46
MS.gene058108	MS.gene073035	0.804871	1.75E-49	-1.69E-46
MS.gene058109	MS.gene073035	0.851333	9.50E-61	-1.69E-46
MS.gene058111	MS.gene073035	0.834099	3.65E-56	-1.69E-46
MS.gene058117	MS.gene073035	0.836356	9.82E-57	-1.69E-46
MS.gene058171	MS.gene073035	0.854029	1.62E-61	-1.69E-46
MS.gene058173	MS.gene073035	0.850249	1.92E-60	-1.69E-46
MS.gene058175	MS.gene073035	0.858771	6.55E-63	-1.69E-46
MS.gene058334	MS.gene073035	0.815061	1.12E-51	-1.69E-46
MS.gene059022	MS.gene073035	-0.804407	2.19E-49	-1.69E-46
MS.gene059045	MS.gene073035	0.813866	2.06E-51	-1.69E-46
MS.gene059865	MS.gene073035	-0.819582	1.08E-52	-1.69E-46
MS.gene060595	MS.gene073035	0.807891	4.05E-50	-1.69E-46
MS.gene060762	MS.gene073035	-0.802291	6.01E-49	-1.69E-46
MS.gene06077	MS.gene073035	-0.82322	1.56E-53	-1.69E-46
MS.gene060982	MS.gene073035	-0.811783	5.89E-51	-1.69E-46
MS.gene061157	MS.gene073035	0.816485	5.40E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073035.t1	MTR_3g109160	98.830	171	2	0	1	171	1	171	2.32e-126	352
MS.gene073035.t1	MTR_1g009150	69.492	177	41	5	5	171	6	179	4.57e-79	233
MS.gene073035.t1	MTR_4g005320	60.294	136	46	2	5	135	4	136	8.95e-45	145
MS.gene073035.t1	MTR_4g021810	70.297	101	23	3	48	141	1	101	1.33e-40	132
MS.gene073035.t1	MTR_2g043960	43.609	133	70	2	10	137	12	144	6.54e-30	107
MS.gene073035.t1	MTR_1g024085	34.615	156	64	3	6	153	4	129	5.58e-23	89.4
MS.gene073035.t1	MTR_4g124750	38.710	124	71	2	14	133	11	133	1.06e-22	89.0
MS.gene073035.t1	MTR_4g072160	36.567	134	49	2	8	140	11	109	3.81e-18	76.6
MS.gene073035.t1	MTR_2g066830	33.613	119	75	1	16	130	1	119	3.52e-17	74.3
MS.gene073035.t1	MTR_4g072570	46.250	80	42	1	56	134	12	91	2.73e-16	70.5
MS.gene073035.t1	MTR_4g072190	41.667	84	46	1	49	132	19	99	6.51e-16	69.7
MS.gene073035.t1	MTR_7g405740	32.576	132	85	2	15	146	15	142	6.52e-16	71.2
MS.gene073035.t1	MTR_4g072910	53.165	79	35	2	51	128	18	95	9.60e-16	69.3
MS.gene073035.t1	MTR_3g084240	53.731	67	30	1	69	134	25	91	1.03e-15	68.9
MS.gene073035.t1	MTR_3g084170	53.731	67	30	1	69	134	25	91	1.29e-15	68.6
MS.gene073035.t1	MTR_3g098970	30.488	164	90	5	10	167	4	149	1.40e-15	70.1
MS.gene073035.t1	MTR_4g050700	52.239	67	31	1	69	134	26	92	1.77e-15	68.6
MS.gene073035.t1	MTR_2g012410	52.239	67	31	1	69	134	26	92	1.77e-15	68.6
MS.gene073035.t1	MTR_3g117610	46.875	64	34	0	69	132	45	108	3.15e-15	68.2
MS.gene073035.t1	MTR_4g072370	54.098	61	27	1	69	128	23	83	5.83e-15	67.0
MS.gene073035.t1	MTR_8g461210	46.970	66	35	0	67	132	33	98	6.28e-15	67.8
MS.gene073035.t1	MTR_3g113310	39.326	89	49	1	49	132	17	105	6.33e-15	67.4
MS.gene073035.t1	MTR_4g072330	54.098	61	27	1	69	128	23	83	6.70e-15	66.6
MS.gene073035.t1	MTR_3g084150	44.706	85	45	2	51	134	8	91	7.60e-15	66.6
MS.gene073035.t1	MTR_4g072880	49.254	67	33	1	69	134	26	92	7.71e-15	66.6
MS.gene073035.t1	MTR_3g084180	44.706	85	45	2	51	134	8	91	8.82e-15	66.6
MS.gene073035.t1	MTR_3g092220	38.000	100	56	2	36	133	4	99	1.11e-14	67.0
MS.gene073035.t1	MTR_5g021820	40.230	87	49	1	49	132	17	103	1.17e-14	66.6
MS.gene073035.t1	MTR_4g072240	44.304	79	43	1	51	128	8	86	1.51e-14	65.9
MS.gene073035.t1	MTR_4g072640	45.455	77	41	1	53	128	9	85	1.62e-14	65.9
MS.gene073035.t1	MTR_8g026730	44.118	68	36	1	68	133	26	93	1.63e-14	66.2
MS.gene073035.t1	MTR_4g072690	53.226	62	28	1	68	128	19	80	2.04e-14	65.5
MS.gene073035.t1	MTR_8g461410	49.254	67	33	1	69	134	25	91	2.56e-14	65.5
MS.gene073035.t1	MTR_3g110042	55.738	61	26	1	69	128	25	85	2.65e-14	65.5
MS.gene073035.t1	MTR_4g072480	43.750	80	44	1	56	134	12	91	3.01e-14	65.1
MS.gene073035.t1	MTR_4g072740	46.753	77	40	1	53	128	4	80	3.19e-14	64.7
MS.gene073035.t1	MTR_5g008370	41.758	91	49	2	48	134	1	91	3.29e-14	66.2
MS.gene073035.t1	MTR_4g072870	47.368	76	38	2	51	125	9	83	3.41e-14	65.1
MS.gene073035.t1	MTR_1g059770	42.029	69	40	0	65	133	45	113	4.18e-14	65.9
MS.gene073035.t1	MTR_4g072220	45.122	82	44	1	48	128	5	86	4.29e-14	64.7
MS.gene073035.t1	MTR_4g072660	50.746	67	32	1	69	134	20	86	4.57e-14	64.7
MS.gene073035.t1	MTR_8g101520	32.540	126	65	2	5	130	6	111	4.60e-14	66.2
MS.gene073035.t1	MTR_3g084220	50.746	67	32	1	69	134	25	91	4.76e-14	64.7
MS.gene073035.t1	MTR_4g072680	42.500	80	45	1	56	134	12	91	6.02e-14	64.3
MS.gene073035.t1	MTR_4g072460	42.500	80	45	1	56	134	12	91	6.14e-14	64.3
MS.gene073035.t1	MTR_4g072470	44.156	77	42	1	53	128	4	80	7.73e-14	63.9
MS.gene073035.t1	MTR_4g072270	42.169	83	47	1	53	134	10	92	7.96e-14	63.9
MS.gene073035.t1	MTR_4g072610	54.098	61	27	1	69	128	19	79	8.10e-14	63.9
MS.gene073035.t1	MTR_4g072500	42.169	83	47	1	53	134	9	91	8.37e-14	63.9
MS.gene073035.t1	MTR_4g072300	42.683	82	46	1	48	128	5	86	8.89e-14	63.9
MS.gene073035.t1	MTR_4g072940	54.098	61	27	1	69	128	57	117	1.04e-13	64.7
MS.gene073035.t1	MTR_7g118010	47.059	68	34	1	68	133	22	89	1.05e-13	63.9
MS.gene073035.t1	MTR_4g072260	46.053	76	40	1	51	125	8	83	1.06e-13	63.9
MS.gene073035.t1	MTR_4g072620	42.500	80	45	1	56	134	12	91	1.09e-13	63.5
MS.gene073035.t1	MTR_4g072650	52.459	61	28	1	69	128	19	79	1.24e-13	63.5
MS.gene073035.t1	MTR_3g084250	55.172	58	25	1	69	125	25	82	1.29e-13	63.5
MS.gene073035.t1	MTR_3g110038	54.098	61	27	1	69	128	25	85	1.44e-13	63.5
MS.gene073035.t1	MTR_4g072730	47.761	67	34	1	69	134	25	91	1.78e-13	63.2
MS.gene073035.t1	MTR_4g072890	47.887	71	36	1	51	120	8	78	1.87e-13	63.2
MS.gene073035.t1	MTR_3g110032	43.373	83	46	1	53	134	10	92	2.57e-13	62.8
MS.gene073035.t1	MTR_7g104940	45.161	62	34	0	72	133	53	114	3.32e-13	63.5
MS.gene073035.t1	MTR_4g072720	52.459	61	28	1	69	128	21	81	4.04e-13	62.0
MS.gene073035.t1	MTR_4g072530	42.857	77	43	1	53	128	9	85	4.05e-13	62.0
MS.gene073035.t1	MTR_4g072310	42.169	83	47	1	53	134	10	92	4.18e-13	62.4
MS.gene073035.t1	MTR_8g076040	36.782	87	52	1	49	132	18	104	4.25e-13	62.4
MS.gene073035.t1	MTR_1g061570	46.970	66	35	0	69	134	19	84	4.63e-13	62.8
MS.gene073035.t1	MTR_4g072490	42.857	77	43	1	53	128	4	80	4.67e-13	62.0
MS.gene073035.t1	MTR_8g101530	45.714	70	38	0	67	136	38	107	4.78e-13	63.5
MS.gene073035.t1	MTR_4g072560	44.186	86	44	3	50	133	15	98	5.11e-13	62.0
MS.gene073035.t1	MTR_4g072820	51.667	60	28	1	67	125	18	77	5.68e-13	61.6
MS.gene073035.t1	MTR_3g084260	53.448	58	26	1	69	125	26	83	6.40e-13	61.6
MS.gene073035.t1	MTR_4g072630	50.000	62	30	1	69	129	21	82	7.33e-13	61.2
MS.gene073035.t1	MTR_4g072510	50.820	61	29	1	69	128	21	81	9.36e-13	61.2
MS.gene073035.t1	MTR_7g104950	43.548	62	35	0	72	133	53	114	1.03e-12	62.0
MS.gene073035.t1	MTR_3g117630	54.098	61	27	1	69	128	25	85	1.10e-12	61.2
MS.gene073035.t1	MTR_4g072540	50.820	61	29	1	69	128	21	81	1.71e-12	60.5
MS.gene073035.t1	MTR_4g072450	50.000	62	30	1	68	128	19	80	1.84e-12	60.5
MS.gene073035.t1	MTR_4g072465	50.820	61	29	1	69	128	21	81	3.31e-12	59.7
MS.gene073035.t1	MTR_4g072850	52.459	61	28	1	69	128	27	87	3.43e-12	59.7
MS.gene073035.t1	MTR_4g072670	50.820	61	29	1	69	128	21	81	4.09e-12	59.3
MS.gene073035.t1	MTR_4g072580	49.180	61	30	1	69	128	20	80	4.19e-12	59.3
MS.gene073035.t1	MTR_4g072590	50.000	58	28	1	69	125	20	77	4.73e-12	59.3
MS.gene073035.t1	MTR_4g025830	42.105	76	40	2	65	136	75	150	5.01e-12	61.2
MS.gene073035.t1	MTR_4g072830	43.590	78	33	2	69	145	23	90	5.88e-12	59.3
MS.gene073035.t1	MTR_3g084200	59.091	44	18	0	69	112	25	68	8.12e-12	59.3
MS.gene073035.t1	MTR_4g072230	52.459	61	26	2	69	128	26	84	1.04e-11	58.5
MS.gene073035.t1	MTR_3g110045	53.448	58	26	1	69	125	84	141	1.32e-11	59.7
MS.gene073035.t1	MTR_0388s0020	35.052	97	58	2	44	137	52	146	1.37e-11	59.7
MS.gene073035.t1	MTR_4g072860	49.254	67	33	1	69	134	26	92	1.38e-11	58.2
MS.gene073035.t1	MTR_4g072600	50.820	61	29	1	69	128	20	80	1.73e-11	57.8
MS.gene073035.t1	MTR_5g008360	40.741	81	47	1	55	134	2	82	2.16e-11	57.4
MS.gene073035.t1	MTR_6g077850	38.554	83	48	2	52	133	19	99	2.63e-11	57.8
MS.gene073035.t1	MTR_4g005310	38.824	85	45	2	52	129	40	124	3.06e-11	58.9
MS.gene073035.t1	MTR_4g072280	50.000	60	30	0	51	110	8	67	3.17e-11	57.0
MS.gene073035.t1	MTR_7g101920	42.105	76	42	1	57	130	23	98	3.57e-11	57.8
MS.gene073035.t1	MTR_1g094750	50.820	61	30	0	69	129	50	110	3.58e-11	57.8
MS.gene073035.t1	MTR_3g084210	46.269	67	35	1	69	134	25	91	3.97e-11	57.0
MS.gene073035.t1	MTR_8g461420	51.724	58	27	1	69	125	25	82	4.18e-11	57.0
MS.gene073035.t1	MTR_5g008350	51.923	52	24	1	70	120	21	72	4.45e-11	56.6
MS.gene073035.t1	MTR_4g072770	45.588	68	36	1	67	133	13	80	6.59e-11	56.2
MS.gene073035.t1	MTR_3g084190	50.000	58	28	1	69	125	25	82	9.13e-11	56.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073035.t1	AT4G31320	53.646	192	58	5	5	171	4	189	1.16e-56	176
MS.gene073035.t1	AT2G24400	54.011	187	54	6	5	171	4	178	7.69e-56	174
MS.gene073035.t1	AT3G43120	40.288	139	75	1	10	140	12	150	2.07e-29	106
MS.gene073035.t1	AT5G20810	44.697	132	65	3	10	133	12	143	1.53e-27	102
MS.gene073035.t1	AT5G20810	44.697	132	65	3	10	133	12	143	1.54e-27	101
MS.gene073035.t1	AT1G19840	37.795	127	50	1	6	132	7	104	4.98e-20	82.0
MS.gene073035.t1	AT5G10990	36.567	134	56	1	6	139	7	111	3.99e-19	79.3
MS.gene073035.t1	AT4G34750	36.220	127	52	2	6	132	3	100	1.16e-18	78.2
MS.gene073035.t1	AT4G34750	36.220	127	52	2	6	132	3	100	1.16e-18	78.2
MS.gene073035.t1	AT3G12830	33.913	115	72	2	16	130	1	111	2.63e-18	77.0
MS.gene073035.t1	AT1G56150	33.913	115	60	1	16	130	1	99	1.24e-17	74.3
MS.gene073035.t1	AT1G79130	35.593	118	64	2	16	130	1	109	2.22e-17	74.3
MS.gene073035.t1	AT1G75590	37.795	127	51	2	6	132	7	105	3.50e-17	74.7
MS.gene073035.t1	AT4G34760	39.326	89	49	1	49	132	18	106	4.21e-17	73.2
MS.gene073035.t1	AT4G36110	42.268	97	54	2	37	131	6	102	1.00e-16	72.0
MS.gene073035.t1	AT3G20220	40.404	99	57	2	32	129	11	108	4.41e-16	70.9
MS.gene073035.t1	AT3G61900	52.239	67	29	1	69	132	30	96	5.37e-16	70.9
MS.gene073035.t1	AT1G75580	35.156	128	59	3	5	132	4	107	3.38e-15	68.2
MS.gene073035.t1	AT2G16580	33.333	120	59	2	19	132	3	107	3.96e-15	67.8
MS.gene073035.t1	AT2G21210	48.193	83	35	3	46	125	10	87	9.56e-15	66.6
MS.gene073035.t1	AT5G66260	40.000	85	50	1	49	132	13	97	1.01e-14	66.6
MS.gene073035.t1	AT2G18010	46.032	63	34	0	69	131	48	110	2.16e-14	66.2
MS.gene073035.t1	AT2G21220	40.230	87	49	1	49	132	17	103	2.44e-14	65.9
MS.gene073035.t1	AT4G38860	39.806	103	56	3	34	132	4	104	2.58e-14	65.9
MS.gene073035.t1	AT2G21210	42.308	104	48	5	33	125	52	154	3.61e-14	67.0
MS.gene073035.t1	AT2G46690	42.857	70	37	1	69	135	24	93	8.68e-14	64.7
MS.gene073035.t1	AT1G19830	31.967	122	63	2	8	129	7	108	1.01e-13	64.7
MS.gene073035.t1	AT4G38840	55.932	59	24	2	69	125	33	91	1.04e-13	63.9
MS.gene073035.t1	AT3G60690	42.683	82	40	2	67	145	86	163	1.74e-13	65.1
MS.gene073035.t1	AT4G00880	47.887	71	35	1	65	133	24	94	1.94e-13	63.9
MS.gene073035.t1	AT4G34770	45.556	90	42	4	51	134	15	103	3.16e-13	62.8
MS.gene073035.t1	AT1G16510	30.833	120	77	2	16	132	1	117	5.85e-13	63.2
MS.gene073035.t1	AT3G51200	46.154	65	34	1	69	132	39	103	6.92e-12	59.3
MS.gene073035.t1	AT4G12410	42.667	75	40	1	65	136	72	146	9.02e-12	60.5
MS.gene073035.t1	AT5G53590	41.429	70	36	1	69	133	46	115	1.15e-11	59.7
MS.gene073035.t1	AT4G34800	49.231	65	29	2	69	129	21	85	1.16e-11	58.5
MS.gene073035.t1	AT3G03850	41.758	91	46	3	51	134	3	93	2.07e-11	57.8
MS.gene073035.t1	AT4G22620	43.056	72	38	1	68	136	78	149	2.99e-11	58.9
MS.gene073035.t1	AT5G18080	41.463	82	42	3	46	125	5	82	3.24e-11	57.4
MS.gene073035.t1	AT4G13790	42.647	68	36	2	68	132	24	91	3.70e-11	57.0
MS.gene073035.t1	AT4G38825	42.857	63	34	2	68	128	22	84	3.71e-11	57.0
MS.gene073035.t1	AT2G36210	31.200	125	75	4	11	133	4	119	9.06e-11	57.4

Find 34 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTTATACCAACTGAGTATT+TGG	0.160089	3.3:+89163947	MS.gene073035:CDS
AGAGAAGCTGAAGAAGAATT+TGG	0.168030	3.3:+89163996	MS.gene073035:CDS
CTACTCTTCTCCATTTCTTA+AGG	0.303486	3.3:-89163749	None:intergenic
GTGTTGGTGTAGACCTTAAC+AGG	0.314144	3.3:+89163922	MS.gene073035:CDS
TCTACTCAGAAATGTAGATT+TGG	0.343922	3.3:+89164107	MS.gene073035:CDS
GAGAAGCTGAAGAAGAATTT+GGG	0.377927	3.3:+89163997	MS.gene073035:CDS
TTCAACAGATCCTTAAGAAA+TGG	0.395955	3.3:+89163739	MS.gene073035:CDS
GAATTTGGGTTTGAACAAAC+TGG	0.403966	3.3:+89164011	MS.gene073035:CDS
TGATCCTCCTCCTTCCCGTT+CGG	0.422045	3.3:-89163865	None:intergenic
TTGGTATAACAAACCTGTTA+AGG	0.460530	3.3:-89163935	None:intergenic
ATTTGGCATTGAAAAGATGA+TGG	0.461164	3.3:+89164124	MS.gene073035:CDS
ATAATAACCAAGTTGGTTTG+AGG	0.468390	3.3:-89164156	None:intergenic
AACACTTTCAATGTCCGAAC+GGG	0.473602	3.3:+89163851	MS.gene073035:CDS
CTTGAAGATGGTTGAAGGAA+AGG	0.483489	3.3:+89164076	MS.gene073035:CDS
TACTGTTCCTCAAACCAACT+TGG	0.509698	3.3:+89164149	MS.gene073035:CDS
GGCTACCTAGCCGTTTGTGT+TGG	0.517754	3.3:+89163906	MS.gene073035:CDS
GTTGATGATAATAACCAAGT+TGG	0.520248	3.3:-89164163	None:intergenic
CAAACGGCTAGGTAGCCTTT+CGG	0.521166	3.3:-89163900	None:intergenic
TATCTGCACATTGGACTATG+AGG	0.522020	3.3:-89164185	None:intergenic
AGTATCTTGAAGATGGTTGA+AGG	0.527345	3.3:+89164071	MS.gene073035:CDS
GTTTGAGAGTATCTTGAAGA+TGG	0.543069	3.3:+89164064	MS.gene073035:CDS
AAGGTCTACACCAACACAAA+CGG	0.549240	3.3:-89163916	None:intergenic
TTTGGCATTGAAAAGATGAT+GGG	0.560948	3.3:+89164125	MS.gene073035:CDS
ATGTCCGAACGGGAAGGAGG+AGG	0.563260	3.3:+89163861	MS.gene073035:CDS
TCAAACAACGCGGTTCCGAA+AGG	0.566250	3.3:+89163885	MS.gene073035:CDS
TTCTCTGAGTAATATATGAA+AGG	0.569528	3.3:-89163979	None:intergenic
TGATGAGCCAAATACTCAGT+TGG	0.592407	3.3:-89163954	None:intergenic
ATGACAAACTATCTGCACAT+TGG	0.610970	3.3:-89164194	None:intergenic
GAACACTTTCAATGTCCGAA+CGG	0.618386	3.3:+89163850	MS.gene073035:CDS
TCAATGTCCGAACGGGAAGG+AGG	0.628171	3.3:+89163858	MS.gene073035:CDS
CTACACCAACACAAACGGCT+AGG	0.632675	3.3:-89163911	None:intergenic
CTTTCAATGTCCGAACGGGA+AGG	0.652936	3.3:+89163855	MS.gene073035:CDS
TCAAACACTGAAACTTCACA+AGG	0.665855	3.3:-89164047	None:intergenic
AGGAGGAGGATCAAACAACG+CGG	0.824181	3.3:+89163875	MS.gene073035:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTCTCTGAGTAATATATGAA+AGG	-	chr3.3:89163982-89164001	None:intergenic	25.0%
!!	ACAATTTCTCTGATTTTGTT+AGG	-	chr3.3:89163714-89163733	None:intergenic	25.0%
!!!	TCTGTTTGTTTTTGAAGTAT+TGG	-	chr3.3:89163775-89163794	None:intergenic	25.0%
	TCTACTCAGAAATGTAGATT+TGG	+	chr3.3:89164107-89164126	MS.gene073035:CDS	30.0%
	TGTTATACCAACTGAGTATT+TGG	+	chr3.3:89163947-89163966	MS.gene073035:CDS	30.0%
	TTCAACAGATCCTTAAGAAA+TGG	+	chr3.3:89163739-89163758	MS.gene073035:CDS	30.0%
	TTGGTATAACAAACCTGTTA+AGG	-	chr3.3:89163938-89163957	None:intergenic	30.0%
!	ATTTGGCATTGAAAAGATGA+TGG	+	chr3.3:89164124-89164143	MS.gene073035:CDS	30.0%
!	ATTTTGTTAGGTTTCTTTGG+TGG	-	chr3.3:89163702-89163721	None:intergenic	30.0%
!	CTGATTTTGTTAGGTTTCTT+TGG	-	chr3.3:89163705-89163724	None:intergenic	30.0%
!	TTTGGCATTGAAAAGATGAT+GGG	+	chr3.3:89164125-89164144	MS.gene073035:CDS	30.0%
!!	ATAATAACCAAGTTGGTTTG+AGG	-	chr3.3:89164159-89164178	None:intergenic	30.0%
!!	GTTGATGATAATAACCAAGT+TGG	-	chr3.3:89164166-89164185	None:intergenic	30.0%
	AGAGAAGCTGAAGAAGAATT+TGG	+	chr3.3:89163996-89164015	MS.gene073035:CDS	35.0%
	AGTATCTTGAAGATGGTTGA+AGG	+	chr3.3:89164071-89164090	MS.gene073035:CDS	35.0%
	CTACTCTTCTCCATTTCTTA+AGG	-	chr3.3:89163752-89163771	None:intergenic	35.0%
	GAGAAGCTGAAGAAGAATTT+GGG	+	chr3.3:89163997-89164016	MS.gene073035:CDS	35.0%
	TCAAACACTGAAACTTCACA+AGG	-	chr3.3:89164050-89164069	None:intergenic	35.0%
!	GAATTTGGGTTTGAACAAAC+TGG	+	chr3.3:89164011-89164030	MS.gene073035:CDS	35.0%
!!	GTTTGAGAGTATCTTGAAGA+TGG	+	chr3.3:89164064-89164083	MS.gene073035:CDS	35.0%
	AACACTTTCAATGTCCGAAC+GGG	+	chr3.3:89163851-89163870	MS.gene073035:CDS	40.0%
	AAGGTCTACACCAACACAAA+CGG	-	chr3.3:89163919-89163938	None:intergenic	40.0%
	CTTGAAGATGGTTGAAGGAA+AGG	+	chr3.3:89164076-89164095	MS.gene073035:CDS	40.0%
	GAACACTTTCAATGTCCGAA+CGG	+	chr3.3:89163850-89163869	MS.gene073035:CDS	40.0%
	TACTGTTCCTCAAACCAACT+TGG	+	chr3.3:89164149-89164168	MS.gene073035:CDS	40.0%
	TATCTGCACATTGGACTATG+AGG	-	chr3.3:89164188-89164207	None:intergenic	40.0%
	TGATGAGCCAAATACTCAGT+TGG	-	chr3.3:89163957-89163976	None:intergenic	40.0%
!	GTGTTGGTGTAGACCTTAAC+AGG	+	chr3.3:89163922-89163941	MS.gene073035:CDS	45.0%
	AGGAGGAGGATCAAACAACG+CGG	+	chr3.3:89163875-89163894	MS.gene073035:CDS	50.0%
	CAAACGGCTAGGTAGCCTTT+CGG	-	chr3.3:89163903-89163922	None:intergenic	50.0%
	CTACACCAACACAAACGGCT+AGG	-	chr3.3:89163914-89163933	None:intergenic	50.0%
	CTTTCAATGTCCGAACGGGA+AGG	+	chr3.3:89163855-89163874	MS.gene073035:CDS	50.0%
	TCAAACAACGCGGTTCCGAA+AGG	+	chr3.3:89163885-89163904	MS.gene073035:CDS	50.0%
	GGCTACCTAGCCGTTTGTGT+TGG	+	chr3.3:89163906-89163925	MS.gene073035:CDS	55.0%
	TCAATGTCCGAACGGGAAGG+AGG	+	chr3.3:89163858-89163877	MS.gene073035:CDS	55.0%
	TGATCCTCCTCCTTCCCGTT+CGG	-	chr3.3:89163868-89163887	None:intergenic	55.0%
	ATGTCCGAACGGGAAGGAGG+AGG	+	chr3.3:89163861-89163880	MS.gene073035:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	89163693	89164208	89163693	ID=MS.gene073035
chr3.3	mRNA	89163693	89164208	89163693	ID=MS.gene073035.t1;Parent=MS.gene073035
chr3.3	exon	89163693	89164208	89163693	ID=MS.gene073035.t1.exon1;Parent=MS.gene073035.t1
chr3.3	CDS	89163693	89164208	89163693	ID=cds.MS.gene073035.t1;Parent=MS.gene073035.t1

Gene Sequence

>MS.gene073035

ATGGATCCACCAAAGAAACCTAACAAAATCAGAGAAATTGTTAGACTTCAACAGATCCTTAAGAAATGGAGAAGAGTAGCCAATACTTCAAAAACAAACAGAAGCAGCAGCAACAATAACAACAGTACTACTAGTAAAAGCATAAAGTTTCTGAAAAGAACACTTTCAATGTCCGAACGGGAAGGAGGAGGATCAAACAACGCGGTTCCGAAAGGCTACCTAGCCGTTTGTGTTGGTGTAGACCTTAACAGGTTTGTTATACCAACTGAGTATTTGGCTCATCAAGCCTTTCATATATTACTCAGAGAAGCTGAAGAAGAATTTGGGTTTGAACAAACTGGTGTTCTGAGAATTCCTTGTGAAGTTTCAGTGTTTGAGAGTATCTTGAAGATGGTTGAAGGAAAGGATAGATTTTCTACTCAGAAATGTAGATTTGGCATTGAAAAGATGATGGGATACTGTTCCTCAAACCAACTTGGTTATTATCATCAACCTCATAGTCCAATGTGCAGATAG

Protein sequence

>MS.gene073035.t1

MDPPKKPNKIREIVRLQQILKKWRRVANTSKTNRSSSNNNNSTTSKSIKFLKRTLSMSEREGGGSNNAVPKGYLAVCVGVDLNRFVIPTEYLAHQAFHILLREAEEEFGFEQTGVLRIPCEVSVFESILKMVEGKDRFSTQKCRFGIEKMMGYCSSNQLGYYHQPHSPMCR