Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08581.t1 | XP_013456038.1 | 90.1 | 617 | 61 | 0 | 1 | 617 | 1 | 617 | 8.00E-238 | 833.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08581.t1 | Q9ZQC5 | 34.4 | 511 | 268 | 9 | 128 | 617 | 119 | 583 | 7.5e-51 | 203.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08581.t1 | A0A072UJY6 | 90.1 | 617 | 61 | 0 | 1 | 617 | 1 | 617 | 5.8e-238 | 833.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049190 | MS.gene08581 | 0.842474 | 2.53E-58 | -1.69E-46 |
| MS.gene049221 | MS.gene08581 | 0.801343 | 9.42E-49 | -1.69E-46 |
| MS.gene049328 | MS.gene08581 | 0.825626 | 4.23E-54 | -1.69E-46 |
| MS.gene049418 | MS.gene08581 | 0.823607 | 1.26E-53 | -1.69E-46 |
| MS.gene050020 | MS.gene08581 | 0.808859 | 2.52E-50 | -1.69E-46 |
| MS.gene050310 | MS.gene08581 | 0.818561 | 1.84E-52 | -1.69E-46 |
| MS.gene050901 | MS.gene08581 | 0.814459 | 1.52E-51 | -1.69E-46 |
| MS.gene051021 | MS.gene08581 | 0.82 | 8.64E-53 | -1.69E-46 |
| MS.gene051199 | MS.gene08581 | 0.815052 | 1.13E-51 | -1.69E-46 |
| MS.gene051248 | MS.gene08581 | 0.809335 | 1.99E-50 | -1.69E-46 |
| MS.gene051478 | MS.gene08581 | 0.801885 | 7.29E-49 | -1.69E-46 |
| MS.gene05157 | MS.gene08581 | 0.841961 | 3.46E-58 | -1.69E-46 |
| MS.gene051719 | MS.gene08581 | 0.839276 | 1.75E-57 | -1.69E-46 |
| MS.gene051720 | MS.gene08581 | 0.85876 | 6.60E-63 | -1.69E-46 |
| MS.gene051744 | MS.gene08581 | 0.81127 | 7.62E-51 | -1.69E-46 |
| MS.gene052133 | MS.gene08581 | 0.808314 | 3.29E-50 | -1.69E-46 |
| MS.gene052665 | MS.gene08581 | 0.821289 | 4.37E-53 | -1.69E-46 |
| MS.gene052731 | MS.gene08581 | 0.831123 | 2.00E-55 | -1.69E-46 |
| MS.gene052732 | MS.gene08581 | 0.877908 | 4.31E-69 | -1.69E-46 |
| MS.gene053144 | MS.gene08581 | 0.878441 | 2.80E-69 | -1.69E-46 |
| MS.gene053174 | MS.gene08581 | 0.852696 | 3.90E-61 | -1.69E-46 |
| MS.gene053581 | MS.gene08581 | 0.809696 | 1.67E-50 | -1.69E-46 |
| MS.gene054550 | MS.gene08581 | 0.856957 | 2.26E-62 | -1.69E-46 |
| MS.gene054584 | MS.gene08581 | 0.88571 | 6.43E-72 | -1.69E-46 |
| MS.gene055144 | MS.gene08581 | 0.808474 | 3.04E-50 | -1.69E-46 |
| MS.gene055465 | MS.gene08581 | 0.843598 | 1.27E-58 | -1.69E-46 |
| MS.gene055466 | MS.gene08581 | 0.82843 | 9.03E-55 | -1.69E-46 |
| MS.gene055468 | MS.gene08581 | 0.819666 | 1.03E-52 | -1.69E-46 |
| MS.gene055537 | MS.gene08581 | 0.842003 | 3.37E-58 | -1.69E-46 |
| MS.gene055580 | MS.gene08581 | -0.83972 | 1.34E-57 | -1.69E-46 |
| MS.gene055890 | MS.gene08581 | 0.840565 | 8.05E-58 | -1.69E-46 |
| MS.gene055891 | MS.gene08581 | 0.831134 | 1.98E-55 | -1.69E-46 |
| MS.gene056043 | MS.gene08581 | -0.811168 | 8.01E-51 | -1.69E-46 |
| MS.gene056228 | MS.gene08581 | 0.827828 | 1.26E-54 | -1.69E-46 |
| MS.gene056507 | MS.gene08581 | 0.802568 | 5.27E-49 | -1.69E-46 |
| MS.gene056553 | MS.gene08581 | 0.815288 | 9.98E-52 | -1.69E-46 |
| MS.gene058087 | MS.gene08581 | 0.804434 | 2.16E-49 | -1.69E-46 |
| MS.gene059154 | MS.gene08581 | 0.830977 | 2.17E-55 | -1.69E-46 |
| MS.gene059297 | MS.gene08581 | 0.811703 | 6.13E-51 | -1.69E-46 |
| MS.gene059300 | MS.gene08581 | 0.800383 | 1.48E-48 | -1.69E-46 |
| MS.gene059937 | MS.gene08581 | 0.817058 | 4.02E-52 | -1.69E-46 |
| MS.gene060355 | MS.gene08581 | 0.800336 | 1.51E-48 | -1.69E-46 |
| MS.gene060519 | MS.gene08581 | 0.846783 | 1.75E-59 | -1.69E-46 |
| MS.gene060587 | MS.gene08581 | 0.820621 | 6.23E-53 | -1.69E-46 |
| MS.gene060603 | MS.gene08581 | 0.803902 | 2.79E-49 | -1.69E-46 |
| MS.gene060884 | MS.gene08581 | 0.818126 | 2.31E-52 | -1.69E-46 |
| MS.gene060886 | MS.gene08581 | 0.812981 | 3.23E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene08581 | MS.gene72021 | PPI |
| MS.gene08581 | MS.gene017922 | PPI |
| MS.gene40254 | MS.gene08581 | PPI |
| MS.gene08581 | MS.gene05873 | PPI |
| MS.gene08581 | MS.gene014608 | PPI |
| MS.gene072335 | MS.gene08581 | PPI |
| MS.gene08581 | MS.gene73762 | PPI |
| MS.gene08581 | MS.gene04811 | PPI |
| MS.gene08581 | MS.gene23577 | PPI |
| MS.gene08581 | MS.gene40704 | PPI |
| MS.gene08581 | MS.gene015800 | PPI |
| MS.gene08581 | MS.gene016702 | PPI |
| MS.gene08581 | MS.gene019335 | PPI |
| MS.gene08581 | MS.gene014143 | PPI |
| MS.gene08581 | MS.gene024179 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08581.t1 | MTR_4g461060 | 97.083 | 617 | 18 | 0 | 1 | 617 | 1 | 617 | 0.0 | 1202 |
| MS.gene08581.t1 | MTR_4g461060 | 97.073 | 615 | 18 | 0 | 3 | 617 | 6 | 620 | 0.0 | 1197 |
| MS.gene08581.t1 | MTR_7g100730 | 45.000 | 620 | 313 | 6 | 4 | 614 | 7 | 607 | 4.98e-139 | 418 |
| MS.gene08581.t1 | MTR_1g066850 | 44.480 | 625 | 285 | 10 | 4 | 614 | 7 | 583 | 2.00e-128 | 390 |
| MS.gene08581.t1 | MTR_2g093260 | 52.349 | 447 | 160 | 9 | 185 | 617 | 78 | 485 | 1.98e-118 | 361 |
| MS.gene08581.t1 | MTR_7g061150 | 46.809 | 376 | 176 | 5 | 245 | 614 | 3 | 360 | 2.33e-80 | 258 |
| MS.gene08581.t1 | MTR_2g093250 | 55.814 | 129 | 53 | 1 | 303 | 431 | 93 | 217 | 3.18e-32 | 125 |
| MS.gene08581.t1 | MTR_2g093240 | 46.296 | 108 | 29 | 2 | 510 | 617 | 3 | 81 | 6.09e-20 | 85.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08581.t1 | AT5G60210 | 44.588 | 619 | 279 | 13 | 3 | 617 | 6 | 564 | 2.69e-144 | 430 |
| MS.gene08581.t1 | AT5G60210 | 44.588 | 619 | 279 | 13 | 3 | 617 | 6 | 564 | 2.69e-144 | 430 |
| MS.gene08581.t1 | AT5G60210 | 44.588 | 619 | 279 | 13 | 3 | 617 | 6 | 564 | 2.69e-144 | 430 |
| MS.gene08581.t1 | AT5G60210 | 44.588 | 619 | 279 | 13 | 3 | 617 | 6 | 564 | 2.69e-144 | 430 |
| MS.gene08581.t1 | AT5G60210 | 44.444 | 621 | 281 | 13 | 1 | 617 | 28 | 588 | 1.89e-143 | 429 |
| MS.gene08581.t1 | AT5G60210 | 44.444 | 621 | 281 | 13 | 1 | 617 | 28 | 588 | 1.89e-143 | 429 |
| MS.gene08581.t1 | AT2G37080 | 40.528 | 644 | 284 | 13 | 4 | 617 | 7 | 581 | 8.06e-117 | 360 |
| MS.gene08581.t1 | AT2G37080 | 40.402 | 646 | 284 | 14 | 4 | 617 | 7 | 583 | 7.20e-115 | 355 |
| MS.gene08581.t1 | AT2G37080 | 40.402 | 646 | 284 | 14 | 4 | 617 | 7 | 583 | 7.20e-115 | 355 |
| MS.gene08581.t1 | AT3G53350 | 39.785 | 186 | 87 | 4 | 3 | 184 | 6 | 170 | 8.64e-27 | 113 |
| MS.gene08581.t1 | AT3G53350 | 30.725 | 345 | 112 | 11 | 274 | 617 | 176 | 394 | 3.33e-23 | 102 |
| MS.gene08581.t1 | AT3G53350 | 39.785 | 186 | 87 | 4 | 3 | 184 | 6 | 170 | 8.64e-27 | 113 |
| MS.gene08581.t1 | AT3G53350 | 30.725 | 345 | 112 | 11 | 274 | 617 | 176 | 394 | 3.33e-23 | 102 |
| MS.gene08581.t1 | AT3G53350 | 40.000 | 185 | 86 | 4 | 4 | 184 | 18 | 181 | 1.97e-26 | 112 |
| MS.gene08581.t1 | AT3G53350 | 30.725 | 345 | 112 | 11 | 274 | 617 | 187 | 405 | 1.07e-22 | 101 |
| MS.gene08581.t1 | AT3G53350 | 39.362 | 188 | 87 | 5 | 3 | 184 | 6 | 172 | 4.27e-25 | 108 |
| MS.gene08581.t1 | AT3G53350 | 30.725 | 345 | 112 | 11 | 274 | 617 | 178 | 396 | 3.48e-23 | 102 |
| MS.gene08581.t1 | AT3G53350 | 39.362 | 188 | 87 | 5 | 3 | 184 | 6 | 172 | 4.27e-25 | 108 |
| MS.gene08581.t1 | AT3G53350 | 30.725 | 345 | 112 | 11 | 274 | 617 | 178 | 396 | 3.48e-23 | 102 |
| MS.gene08581.t1 | AT3G53350 | 39.362 | 188 | 87 | 5 | 3 | 184 | 6 | 172 | 4.27e-25 | 108 |
| MS.gene08581.t1 | AT3G53350 | 30.725 | 345 | 112 | 11 | 274 | 617 | 178 | 396 | 3.48e-23 | 102 |
| MS.gene08581.t1 | AT3G53350 | 39.572 | 187 | 86 | 5 | 4 | 184 | 18 | 183 | 1.03e-24 | 107 |
| MS.gene08581.t1 | AT3G53350 | 30.725 | 345 | 112 | 11 | 274 | 617 | 189 | 407 | 8.89e-23 | 101 |
| MS.gene08581.t1 | AT3G53350 | 39.572 | 187 | 86 | 5 | 4 | 184 | 18 | 183 | 1.03e-24 | 107 |
| MS.gene08581.t1 | AT3G53350 | 30.725 | 345 | 112 | 11 | 274 | 617 | 189 | 407 | 8.89e-23 | 101 |
| MS.gene08581.t1 | AT3G53350 | 30.725 | 345 | 112 | 11 | 274 | 617 | 206 | 424 | 9.87e-23 | 101 |
| MS.gene08581.t1 | AT3G53350 | 35.185 | 216 | 85 | 5 | 3 | 184 | 6 | 200 | 3.40e-21 | 97.1 |
| MS.gene08581.t1 | AT1G78430 | 37.079 | 178 | 100 | 5 | 441 | 614 | 151 | 320 | 3.77e-18 | 86.3 |
| MS.gene08581.t1 | AT1G17140 | 34.254 | 181 | 98 | 4 | 446 | 614 | 169 | 340 | 5.05e-14 | 74.3 |
| MS.gene08581.t1 | AT1G17140 | 34.254 | 181 | 98 | 4 | 446 | 614 | 169 | 340 | 5.05e-14 | 74.3 |
Find 103 sgRNAs with CRISPR-Local
Find 129 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGATTTGTGCCTGAGTTTC+AGG | 0.158972 | 4.1:+38294477 | None:intergenic |
| TACCTCAGGAACTGGGCTTC+TGG | 0.169803 | 4.1:+38295429 | None:intergenic |
| AAAGAACCGTCGAGATTCTA+AGG | 0.289339 | 4.1:-38294419 | MS.gene08581:CDS |
| TACTGAGAGATCCGTGTCTT+TGG | 0.303034 | 4.1:-38293464 | MS.gene08581:CDS |
| GAGCAACTCTTAGCCCTATC+TGG | 0.309073 | 4.1:-38294840 | MS.gene08581:CDS |
| TCAACATCTTCAGCATAATT+AGG | 0.312004 | 4.1:+38293415 | None:intergenic |
| ACCTCAGGAACTGGGCTTCT+GGG | 0.316608 | 4.1:+38295430 | None:intergenic |
| TCTTAGTTCGCTTTCCATTT+CGG | 0.319511 | 4.1:+38293570 | None:intergenic |
| TGCTTTGTCTTCTGCATTGA+AGG | 0.331152 | 4.1:-38294667 | MS.gene08581:CDS |
| ATAATTAGGTGAATACTTAT+AGG | 0.350218 | 4.1:+38293429 | None:intergenic |
| CGGTCACCCTCGCCACTTTA+CGG | 0.350515 | 4.1:+38293623 | None:intergenic |
| TGACTTGCGATCAGCGAGTT+TGG | 0.351529 | 4.1:+38295453 | None:intergenic |
| GGTAACATGCTGAAGAAGTT+TGG | 0.353831 | 4.1:-38293367 | MS.gene08581:CDS |
| GCACGCGGAGAAACTTTCTT+AGG | 0.360740 | 4.1:+38295568 | None:intergenic |
| ATCTCTTCTTCAATATGATT+AGG | 0.361618 | 4.1:+38294192 | None:intergenic |
| AAAGTGAAATATTGAGAAAT+GGG | 0.363571 | 4.1:-38294221 | MS.gene08581:CDS |
| CCCGAGCAAAATATCTGAAT+TGG | 0.366490 | 4.1:-38294970 | MS.gene08581:CDS |
| CAGCAATGCGTACGAGTTGA+TGG | 0.372307 | 4.1:-38294073 | MS.gene08581:CDS |
| GAAAGTGAAATATTGAGAAA+TGG | 0.381211 | 4.1:-38294222 | MS.gene08581:CDS |
| TGAAGAAGTTTGGAGTCTTA+TGG | 0.383316 | 4.1:-38293357 | MS.gene08581:CDS |
| GTTCTTTGCTATTCTTGAGT+TGG | 0.389004 | 4.1:+38294499 | None:intergenic |
| CAACTCCATTGCTTTGGAGT+TGG | 0.399162 | 4.1:-38294367 | MS.gene08581:CDS |
| AAACTTGTTACCTCAGGAAC+TGG | 0.401038 | 4.1:+38295421 | None:intergenic |
| GGCAGCTGCTGCTATGATTT+CGG | 0.401359 | 4.1:-38293506 | MS.gene08581:CDS |
| TCAAAAGGAACTCAAAAGAT+TGG | 0.417608 | 4.1:-38293857 | MS.gene08581:CDS |
| TGGCTGCAACTCCATTGCTT+TGG | 0.418359 | 4.1:-38294373 | MS.gene08581:CDS |
| CGCGAGGCGGTTACGAAACT+CGG | 0.423677 | 4.1:-38293676 | MS.gene08581:CDS |
| CTTTAGCATCTCGTTCTCTT+CGG | 0.425187 | 4.1:+38293771 | None:intergenic |
| GCTGAAAGCACAACTTGAAT+TGG | 0.425528 | 4.1:-38294634 | MS.gene08581:CDS |
| AACTGATATTGAAGAGTTGA+AGG | 0.430741 | 4.1:-38293986 | MS.gene08581:CDS |
| TGTTCTTTGAAGTCTGAGAT+TGG | 0.431751 | 4.1:-38294168 | MS.gene08581:CDS |
| TATCTAGAGCTATGTGCTAC+TGG | 0.432289 | 4.1:-38294792 | MS.gene08581:CDS |
| AGAGTTGAAGGCTAATCTAA+TGG | 0.435601 | 4.1:-38293974 | MS.gene08581:CDS |
| CCTCCAACTCACATTGTCTC+TGG | 0.446526 | 4.1:+38294028 | None:intergenic |
| AAATGAACATGGACTTCAAA+AGG | 0.449057 | 4.1:-38293872 | MS.gene08581:CDS |
| GCAAGCTCAGCAAGACTTCG+AGG | 0.450128 | 4.1:-38294871 | MS.gene08581:CDS |
| GTTGATGGAGCAGATAAAAT+CGG | 0.470705 | 4.1:-38294058 | MS.gene08581:CDS |
| GAGCAGATAAAATCGGAATC+AGG | 0.477930 | 4.1:-38294051 | MS.gene08581:CDS |
| AACCGAGTTGCAAGGTATCA+TGG | 0.481196 | 4.1:-38293944 | MS.gene08581:CDS |
| AAGCGAACTAAGAAGGCTTA+AGG | 0.481520 | 4.1:-38293560 | MS.gene08581:CDS |
| TAGGGCTAAGAGTTGCTCCT+TGG | 0.485611 | 4.1:+38294845 | None:intergenic |
| TCAAACTCAACAAGCTGAAT+CGG | 0.486664 | 4.1:-38294592 | MS.gene08581:CDS |
| CAGAAGTGGTTCTTCTGAAG+TGG | 0.492122 | 4.1:-38295593 | MS.gene08581:CDS |
| ACAAGTGGCAAGCTCCGAAA+TGG | 0.492854 | 4.1:-38293584 | MS.gene08581:CDS |
| TCATTCTGTTCAAAATCTTG+TGG | 0.494055 | 4.1:-38294265 | MS.gene08581:CDS |
| GTCAACTGAGAGATGCTGTT+GGG | 0.495983 | 4.1:+38294695 | None:intergenic |
| GAAAATCAACAAGAGTTGCT+TGG | 0.499437 | 4.1:-38293746 | MS.gene08581:CDS |
| CCAGAGACAATGTGAGTTGG+AGG | 0.499761 | 4.1:-38294028 | MS.gene08581:CDS |
| TGATTTCGGCAGGGAACAAT+GGG | 0.505513 | 4.1:-38293492 | MS.gene08581:CDS |
| ACTTCAAGATGATTTGAAGA+AGG | 0.507940 | 4.1:-38294931 | MS.gene08581:CDS |
| GCTGCTGCTATGATTTCGGC+AGG | 0.511645 | 4.1:-38293502 | MS.gene08581:CDS |
| ATGATTTCGGCAGGGAACAA+TGG | 0.513538 | 4.1:-38293493 | MS.gene08581:CDS |
| TAGCAAAGAACCTGAAACTC+AGG | 0.524331 | 4.1:-38294487 | MS.gene08581:CDS |
| CTTTGAAGTCTGAGATTGGA+CGG | 0.524621 | 4.1:-38294164 | MS.gene08581:CDS |
| GACGTTGCGAGAGACGTAGA+TGG | 0.528471 | 4.1:-38294393 | MS.gene08581:CDS |
| ACGTGATAGCGCATGGAAGT+CGG | 0.536408 | 4.1:-38294730 | MS.gene08581:CDS |
| AAATGGAAAGCGAACTAAGA+AGG | 0.538764 | 4.1:-38293567 | MS.gene08581:CDS |
| TTCCATGATACCTTGCAACT+CGG | 0.542413 | 4.1:+38293942 | None:intergenic |
| AGACAGAAGCAACCGTAAAG+TGG | 0.543135 | 4.1:-38293635 | MS.gene08581:CDS |
| ATCAGAATCACTTTCTCAGA+TGG | 0.543609 | 4.1:-38294535 | MS.gene08581:CDS |
| GCAGTTAGAAGCAGCACAAG+TGG | 0.544851 | 4.1:-38293599 | MS.gene08581:CDS |
| TTGTGCTGCTTCTAACTGCT+CGG | 0.545994 | 4.1:+38293603 | None:intergenic |
| TTGTTCTTGATATTTAGTCT+CGG | 0.554617 | 4.1:+38294119 | None:intergenic |
| AACTTGTTACCTCAGGAACT+GGG | 0.572563 | 4.1:+38295422 | None:intergenic |
| AGATGATTTGAAGAAGGTGA+AGG | 0.573247 | 4.1:-38294925 | MS.gene08581:CDS |
| AGGCCAGAGACAATGTGAGT+TGG | 0.574082 | 4.1:-38294031 | MS.gene08581:CDS |
| AGTTGGAACATTCTAGAGCA+AGG | 0.576110 | 4.1:-38294350 | MS.gene08581:CDS |
| CAGAAGAACGTGATAGCGCA+TGG | 0.577018 | 4.1:-38294737 | MS.gene08581:CDS |
| ATGATTCAGACAAAATAAGC+TGG | 0.579255 | 4.1:+38294901 | None:intergenic |
| TCAAACTAGTAAAATATCAA+AGG | 0.580215 | 4.1:-38295485 | MS.gene08581:CDS |
| GCAAGACTTCGAGGAATCCA+AGG | 0.582107 | 4.1:-38294862 | MS.gene08581:CDS |
| TACGAAACTCGGGATTGTGA+TGG | 0.584576 | 4.1:-38293665 | MS.gene08581:CDS |
| GAAACTCGGGATTGTGATGG+AGG | 0.585195 | 4.1:-38293662 | MS.gene08581:CDS |
| ATGTTCCAACTCCAAAGCAA+TGG | 0.588382 | 4.1:+38294362 | None:intergenic |
| TTAAGGTACAGTCTGATCAA+TGG | 0.588594 | 4.1:-38293543 | MS.gene08581:CDS |
| AGCCGAGGCTGCAAAACGCG+AGG | 0.589775 | 4.1:-38293692 | MS.gene08581:CDS |
| CTTGAATTGGTAGCTACTTG+TGG | 0.590895 | 4.1:-38294621 | MS.gene08581:CDS |
| TGAATTGAAAGCTGATATGA+TGG | 0.591704 | 4.1:-38293821 | MS.gene08581:CDS |
| GGATGACAGTAAAGTGAGAG+AGG | 0.601699 | 4.1:-38293725 | MS.gene08581:CDS |
| TGAAATATTGAGAAATGGGG+AGG | 0.601938 | 4.1:-38294217 | MS.gene08581:CDS |
| GAGTAGTCGTAGTTCTCAGT+TGG | 0.604285 | 4.1:+38295539 | None:intergenic |
| GGATGATTGTAAATTCGAGA+AGG | 0.605294 | 4.1:-38294244 | MS.gene08581:CDS |
| GTTGGAACATTCTAGAGCAA+GGG | 0.613738 | 4.1:-38294349 | MS.gene08581:CDS |
| ACCCAGAAGCCCAGTTCCTG+AGG | 0.615333 | 4.1:-38295431 | MS.gene08581:intron |
| ATGTCATCACAAAATGAACA+TGG | 0.616779 | 4.1:-38293883 | MS.gene08581:CDS |
| CTCAGTTGGCGTACAGCACG+CGG | 0.616898 | 4.1:+38295553 | None:intergenic |
| GACAAGGAAACCGAGTTGCA+AGG | 0.617966 | 4.1:-38293952 | MS.gene08581:CDS |
| CGAGGCTGCAAAACGCGAGG+CGG | 0.620966 | 4.1:-38293689 | MS.gene08581:CDS |
| AAGCAACCGTAAAGTGGCGA+GGG | 0.625212 | 4.1:-38293629 | MS.gene08581:CDS |
| ACAGTCTGATCAATGGAGGA+AGG | 0.630704 | 4.1:-38293536 | MS.gene08581:CDS |
| GCGAGGCGGTTACGAAACTC+GGG | 0.631591 | 4.1:-38293675 | MS.gene08581:CDS |
| AGTCAACTGAGAGATGCTGT+TGG | 0.631624 | 4.1:+38294694 | None:intergenic |
| ATTATTATTGTCCAAAGACA+CGG | 0.631830 | 4.1:+38293453 | None:intergenic |
| AAGTGAAATATTGAGAAATG+GGG | 0.635977 | 4.1:-38294220 | MS.gene08581:CDS |
| TCAATGGAGGAAGGCTGCAG+AGG | 0.637395 | 4.1:-38293527 | MS.gene08581:CDS |
| GAAGCAACCGTAAAGTGGCG+AGG | 0.639605 | 4.1:-38293630 | MS.gene08581:CDS |
| GAAGGCTAATCTAATGGACA+AGG | 0.644778 | 4.1:-38293968 | MS.gene08581:CDS |
| ATTGTTTAATAATGACAACA+AGG | 0.651081 | 4.1:-38295703 | None:intergenic |
| AGGTACAGTCTGATCAATGG+AGG | 0.656687 | 4.1:-38293540 | MS.gene08581:CDS |
| GTCATCCCTTAGAATCTCGA+CGG | 0.658533 | 4.1:+38294413 | None:intergenic |
| CTGCTGCTATGATTTCGGCA+GGG | 0.663851 | 4.1:-38293501 | MS.gene08581:CDS |
| AGAGGAAATAGAGAAAGCCG+AGG | 0.674506 | 4.1:-38293707 | MS.gene08581:CDS |
| AAGAACCGTCGAGATTCTAA+GGG | 0.684529 | 4.1:-38294418 | MS.gene08581:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAATGAAAGATAAATTAA+AGG | - | chr4.1:38293722-38293741 | MS.gene08581:CDS | 10.0% |
| !! | TATTGAATAAAAGTTATACA+AGG | + | chr4.1:38293940-38293959 | None:intergenic | 15.0% |
| !! | TTTATCTTTCATTATAGAAA+TGG | + | chr4.1:38293718-38293737 | None:intergenic | 15.0% |
| !! | AAAGTGAAATATTGAGAAAT+GGG | - | chr4.1:38294810-38294829 | MS.gene08581:CDS | 20.0% |
| !! | ATAATTAGGTGAATACTTAT+AGG | + | chr4.1:38295605-38295624 | None:intergenic | 20.0% |
| !! | TACAAAATCTATGTTGTAAA+TGG | + | chr4.1:38293388-38293407 | None:intergenic | 20.0% |
| !! | TCAAACTAGTAAAATATCAA+AGG | - | chr4.1:38293546-38293565 | MS.gene08581:CDS | 20.0% |
| !!! | ATTCTGATAAGTTTTGATTT+AGG | + | chr4.1:38294484-38294503 | None:intergenic | 20.0% |
| !!! | CAGAAGTAGTATTTTATTAT+TGG | + | chr4.1:38293639-38293658 | None:intergenic | 20.0% |
| !!! | TTTTATTGAAATTTCAGAAG+TGG | - | chr4.1:38293424-38293443 | MS.gene08581:CDS | 20.0% |
| ! | AAGTGAAATATTGAGAAATG+GGG | - | chr4.1:38294811-38294830 | MS.gene08581:CDS | 25.0% |
| ! | ACAACAAGGTATAAATTGAT+TGG | - | chr4.1:38293342-38293361 | MS.gene08581:CDS | 25.0% |
| ! | ATCTCTTCTTCAATATGATT+AGG | + | chr4.1:38294842-38294861 | None:intergenic | 25.0% |
| ! | ATTATTATTGTCCAAAGACA+CGG | + | chr4.1:38295581-38295600 | None:intergenic | 25.0% |
| ! | CCAAACAAAGTCTATATTTA+AGG | + | chr4.1:38293663-38293682 | None:intergenic | 25.0% |
| ! | CCTTAAATATAGACTTTGTT+TGG | - | chr4.1:38293660-38293679 | MS.gene08581:CDS | 25.0% |
| ! | GAAAGTGAAATATTGAGAAA+TGG | - | chr4.1:38294809-38294828 | MS.gene08581:CDS | 25.0% |
| ! | TATAATTGATGTTGCATTAG+AGG | - | chr4.1:38294033-38294052 | MS.gene08581:CDS | 25.0% |
| ! | TTGTTCTTGATATTTAGTCT+CGG | + | chr4.1:38294915-38294934 | None:intergenic | 25.0% |
| !!! | ATTAAGTTGATTATCCAAAC+TGG | - | chr4.1:38293913-38293932 | MS.gene08581:CDS | 25.0% |
| !!! | TTAAGTTGATTATCCAAACT+GGG | - | chr4.1:38293914-38293933 | MS.gene08581:CDS | 25.0% |
| AAATGAACATGGACTTCAAA+AGG | - | chr4.1:38295159-38295178 | MS.gene08581:intron | 30.0% | |
| AACTGATATTGAAGAGTTGA+AGG | - | chr4.1:38295045-38295064 | MS.gene08581:intron | 30.0% | |
| ACTTCAAGATGATTTGAAGA+AGG | - | chr4.1:38294100-38294119 | MS.gene08581:CDS | 30.0% | |
| ATGATTCAGACAAAATAAGC+TGG | + | chr4.1:38294133-38294152 | None:intergenic | 30.0% | |
| ATGTCATCACAAAATGAACA+TGG | - | chr4.1:38295148-38295167 | MS.gene08581:intron | 30.0% | |
| GATTTCCAGAGAAAGAAAAA+TGG | - | chr4.1:38295643-38295662 | MS.gene08581:intron | 30.0% | |
| TCAAAAGGAACTCAAAAGAT+TGG | - | chr4.1:38295174-38295193 | MS.gene08581:intron | 30.0% | |
| TCAACATCTTCAGCATAATT+AGG | + | chr4.1:38295619-38295638 | None:intergenic | 30.0% | |
| TCATTCTGTTCAAAATCTTG+TGG | - | chr4.1:38294766-38294785 | MS.gene08581:CDS | 30.0% | |
| TGAATTGAAAGCTGATATGA+TGG | - | chr4.1:38295210-38295229 | MS.gene08581:intron | 30.0% | |
| ! | TCCAATTCAGATATTTTGCT+CGG | + | chr4.1:38294065-38294084 | None:intergenic | 30.0% |
| !! | TGTTACCATTTTTCTTTCTC+TGG | + | chr4.1:38295651-38295670 | None:intergenic | 30.0% |
| AAATGGAAAGCGAACTAAGA+AGG | - | chr4.1:38295464-38295483 | MS.gene08581:CDS | 35.0% | |
| AGAGTTGAAGGCTAATCTAA+TGG | - | chr4.1:38295057-38295076 | MS.gene08581:intron | 35.0% | |
| AGATGATTTGAAGAAGGTGA+AGG | - | chr4.1:38294106-38294125 | MS.gene08581:CDS | 35.0% | |
| ATCAGAATCACTTTCTCAGA+TGG | - | chr4.1:38294496-38294515 | MS.gene08581:CDS | 35.0% | |
| GAAAATCAACAAGAGTTGCT+TGG | - | chr4.1:38295285-38295304 | MS.gene08581:intron | 35.0% | |
| GGATGATTGTAAATTCGAGA+AGG | - | chr4.1:38294787-38294806 | MS.gene08581:CDS | 35.0% | |
| TCAAACTCAACAAGCTGAAT+CGG | - | chr4.1:38294439-38294458 | MS.gene08581:CDS | 35.0% | |
| TCTTAGTTCGCTTTCCATTT+CGG | + | chr4.1:38295464-38295483 | None:intergenic | 35.0% | |
| TGAAATATTGAGAAATGGGG+AGG | - | chr4.1:38294814-38294833 | MS.gene08581:CDS | 35.0% | |
| TTAAGGTACAGTCTGATCAA+TGG | - | chr4.1:38295488-38295507 | MS.gene08581:CDS | 35.0% | |
| ! | CCAATTCAGATATTTTGCTC+GGG | + | chr4.1:38294064-38294083 | None:intergenic | 35.0% |
| ! | GTTGATGGAGCAGATAAAAT+CGG | - | chr4.1:38294973-38294992 | MS.gene08581:CDS | 35.0% |
| ! | TGAAGAAGTTTGGAGTCTTA+TGG | - | chr4.1:38295674-38295693 | MS.gene08581:intron | 35.0% |
| !! | GTTCTTTGCTATTCTTGAGT+TGG | + | chr4.1:38294535-38294554 | None:intergenic | 35.0% |
| !! | TGGTAAAAACTTGTTACCTC+AGG | + | chr4.1:38293619-38293638 | None:intergenic | 35.0% |
| !! | TGTTCTTTGAAGTCTGAGAT+TGG | - | chr4.1:38294863-38294882 | MS.gene08581:CDS | 35.0% |
| AAAGAACCGTCGAGATTCTA+AGG | - | chr4.1:38294612-38294631 | MS.gene08581:CDS | 40.0% | |
| AAGAACCGTCGAGATTCTAA+GGG | - | chr4.1:38294613-38294632 | MS.gene08581:CDS | 40.0% | |
| AAGATTTGTGCCTGAGTTTC+AGG | + | chr4.1:38294557-38294576 | None:intergenic | 40.0% | |
| AAGCACTTGTGAGACTGTTT+GGG | + | chr4.1:38293803-38293822 | None:intergenic | 40.0% | |
| AAGCGAACTAAGAAGGCTTA+AGG | - | chr4.1:38295471-38295490 | MS.gene08581:CDS | 40.0% | |
| AAGTTATACAAGGCCCAGTT+TGG | + | chr4.1:38293930-38293949 | None:intergenic | 40.0% | |
| AGTCTCACAAGTGCTTATGT+CGG | - | chr4.1:38293807-38293826 | MS.gene08581:CDS | 40.0% | |
| ATGTTCCAACTCCAAAGCAA+TGG | + | chr4.1:38294672-38294691 | None:intergenic | 40.0% | |
| CACATTGCTAGCATTGCTTT+CGG | - | chr4.1:38293972-38293991 | MS.gene08581:CDS | 40.0% | |
| CCCGAGCAAAATATCTGAAT+TGG | - | chr4.1:38294061-38294080 | MS.gene08581:CDS | 40.0% | |
| CTTTAGCATCTCGTTCTCTT+CGG | + | chr4.1:38295263-38295282 | None:intergenic | 40.0% | |
| GAGCAGATAAAATCGGAATC+AGG | - | chr4.1:38294980-38294999 | MS.gene08581:CDS | 40.0% | |
| GGTAACATGCTGAAGAAGTT+TGG | - | chr4.1:38295664-38295683 | MS.gene08581:intron | 40.0% | |
| TAAGCACTTGTGAGACTGTT+TGG | + | chr4.1:38293804-38293823 | None:intergenic | 40.0% | |
| TAGCAAAGAACCTGAAACTC+AGG | - | chr4.1:38294544-38294563 | MS.gene08581:CDS | 40.0% | |
| TGCTTTGTCTTCTGCATTGA+AGG | - | chr4.1:38294364-38294383 | MS.gene08581:CDS | 40.0% | |
| TTCCATGATACCTTGCAACT+CGG | + | chr4.1:38295092-38295111 | None:intergenic | 40.0% | |
| ! | CACGTTCTTCTGTGATTTTC+TGG | + | chr4.1:38294286-38294305 | None:intergenic | 40.0% |
| ! | CTTGAATTGGTAGCTACTTG+TGG | - | chr4.1:38294410-38294429 | MS.gene08581:CDS | 40.0% |
| ! | GAAGGCTAATCTAATGGACA+AGG | - | chr4.1:38295063-38295082 | MS.gene08581:intron | 40.0% |
| ! | GCTGAAAGCACAACTTGAAT+TGG | - | chr4.1:38294397-38294416 | MS.gene08581:CDS | 40.0% |
| ! | TATCTAGAGCTATGTGCTAC+TGG | - | chr4.1:38294239-38294258 | MS.gene08581:CDS | 40.0% |
| !! | AAACTTGTTACCTCAGGAAC+TGG | + | chr4.1:38293613-38293632 | None:intergenic | 40.0% |
| !! | AACTTGTTACCTCAGGAACT+GGG | + | chr4.1:38293612-38293631 | None:intergenic | 40.0% |
| !! | ACTCTTCGAGTTTTCCAGAT+AGG | + | chr4.1:38294208-38294227 | None:intergenic | 40.0% |
| !! | AGTTGGAACATTCTAGAGCA+AGG | - | chr4.1:38294681-38294700 | MS.gene08581:CDS | 40.0% |
| !! | CTCTTCGAGTTTTCCAGATA+GGG | + | chr4.1:38294207-38294226 | None:intergenic | 40.0% |
| !! | CTTTGAAGTCTGAGATTGGA+CGG | - | chr4.1:38294867-38294886 | MS.gene08581:CDS | 40.0% |
| !! | GTTGGAACATTCTAGAGCAA+GGG | - | chr4.1:38294682-38294701 | MS.gene08581:CDS | 40.0% |
| ACAGTCTGATCAATGGAGGA+AGG | - | chr4.1:38295495-38295514 | MS.gene08581:CDS | 45.0% | |
| AGACAGAAGCAACCGTAAAG+TGG | - | chr4.1:38295396-38295415 | MS.gene08581:intron | 45.0% | |
| AGAGGAAATAGAGAAAGCCG+AGG | - | chr4.1:38295324-38295343 | MS.gene08581:intron | 45.0% | |
| AGGTACAGTCTGATCAATGG+AGG | - | chr4.1:38295491-38295510 | MS.gene08581:CDS | 45.0% | |
| AGTCAACTGAGAGATGCTGT+TGG | + | chr4.1:38294340-38294359 | None:intergenic | 45.0% | |
| ATGATTTCGGCAGGGAACAA+TGG | - | chr4.1:38295538-38295557 | MS.gene08581:CDS | 45.0% | |
| GAGTAGTCGTAGTTCTCAGT+TGG | + | chr4.1:38293495-38293514 | None:intergenic | 45.0% | |
| GGATGACAGTAAAGTGAGAG+AGG | - | chr4.1:38295306-38295325 | MS.gene08581:intron | 45.0% | |
| GTCAACTGAGAGATGCTGTT+GGG | + | chr4.1:38294339-38294358 | None:intergenic | 45.0% | |
| GTCATCCCTTAGAATCTCGA+CGG | + | chr4.1:38294621-38294640 | None:intergenic | 45.0% | |
| TACGAAACTCGGGATTGTGA+TGG | - | chr4.1:38295366-38295385 | MS.gene08581:intron | 45.0% | |
| TACTGAGAGATCCGTGTCTT+TGG | - | chr4.1:38295567-38295586 | MS.gene08581:CDS | 45.0% | |
| TGATTTCGGCAGGGAACAAT+GGG | - | chr4.1:38295539-38295558 | MS.gene08581:CDS | 45.0% | |
| ! | AACCGAGTTGCAAGGTATCA+TGG | - | chr4.1:38295087-38295106 | MS.gene08581:intron | 45.0% |
| ! | CAGAAGTGGTTCTTCTGAAG+TGG | - | chr4.1:38293438-38293457 | MS.gene08581:CDS | 45.0% |
| !! | CAACTCCATTGCTTTGGAGT+TGG | - | chr4.1:38294664-38294683 | MS.gene08581:CDS | 45.0% |
| !! | TTGTGCTGCTTCTAACTGCT+CGG | + | chr4.1:38295431-38295450 | None:intergenic | 45.0% |
| AAGCAACCGTAAAGTGGCGA+GGG | - | chr4.1:38295402-38295421 | MS.gene08581:intron | 50.0% | |
| ACAAGTGGCAAGCTCCGAAA+TGG | - | chr4.1:38295447-38295466 | MS.gene08581:CDS | 50.0% | |
| AGGCCAGAGACAATGTGAGT+TGG | - | chr4.1:38295000-38295019 | MS.gene08581:intron | 50.0% | |
| CCTCCAACTCACATTGTCTC+TGG | + | chr4.1:38295006-38295025 | None:intergenic | 50.0% | |
| CTGCTGCTATGATTTCGGCA+GGG | - | chr4.1:38295530-38295549 | MS.gene08581:CDS | 50.0% | |
| GAAACTCGGGATTGTGATGG+AGG | - | chr4.1:38295369-38295388 | MS.gene08581:intron | 50.0% | |
| GACAAGGAAACCGAGTTGCA+AGG | - | chr4.1:38295079-38295098 | MS.gene08581:intron | 50.0% | |
| GAGCAACTCTTAGCCCTATC+TGG | - | chr4.1:38294191-38294210 | MS.gene08581:CDS | 50.0% | |
| GCAAGACTTCGAGGAATCCA+AGG | - | chr4.1:38294169-38294188 | MS.gene08581:CDS | 50.0% | |
| GCACGCGGAGAAACTTTCTT+AGG | + | chr4.1:38293466-38293485 | None:intergenic | 50.0% | |
| GCAGTTAGAAGCAGCACAAG+TGG | - | chr4.1:38295432-38295451 | MS.gene08581:CDS | 50.0% | |
| GGCAGCTGCTGCTATGATTT+CGG | - | chr4.1:38295525-38295544 | MS.gene08581:CDS | 50.0% | |
| TAGGGCTAAGAGTTGCTCCT+TGG | + | chr4.1:38294189-38294208 | None:intergenic | 50.0% | |
| TGGCTGCAACTCCATTGCTT+TGG | - | chr4.1:38294658-38294677 | MS.gene08581:CDS | 50.0% | |
| ! | ACGTGATAGCGCATGGAAGT+CGG | - | chr4.1:38294301-38294320 | MS.gene08581:CDS | 50.0% |
| ! | CAGAAGAACGTGATAGCGCA+TGG | - | chr4.1:38294294-38294313 | MS.gene08581:CDS | 50.0% |
| ! | CAGCAATGCGTACGAGTTGA+TGG | - | chr4.1:38294958-38294977 | MS.gene08581:CDS | 50.0% |
| ! | CCAGAGACAATGTGAGTTGG+AGG | - | chr4.1:38295003-38295022 | MS.gene08581:intron | 50.0% |
| ! | TGACTTGCGATCAGCGAGTT+TGG | + | chr4.1:38293581-38293600 | None:intergenic | 50.0% |
| ACCTCAGGAACTGGGCTTCT+GGG | + | chr4.1:38293604-38293623 | None:intergenic | 55.0% | |
| GAAGCAACCGTAAAGTGGCG+AGG | - | chr4.1:38295401-38295420 | MS.gene08581:intron | 55.0% | |
| GACGTTGCGAGAGACGTAGA+TGG | - | chr4.1:38294638-38294657 | MS.gene08581:CDS | 55.0% | |
| GCAAGCTCAGCAAGACTTCG+AGG | - | chr4.1:38294160-38294179 | MS.gene08581:CDS | 55.0% | |
| GCTGCTGCTATGATTTCGGC+AGG | - | chr4.1:38295529-38295548 | MS.gene08581:CDS | 55.0% | |
| TACCTCAGGAACTGGGCTTC+TGG | + | chr4.1:38293605-38293624 | None:intergenic | 55.0% | |
| TCAATGGAGGAAGGCTGCAG+AGG | - | chr4.1:38295504-38295523 | MS.gene08581:CDS | 55.0% | |
| ACCCAGAAGCCCAGTTCCTG+AGG | - | chr4.1:38293600-38293619 | MS.gene08581:CDS | 60.0% | |
| CGCGAGGCGGTTACGAAACT+CGG | - | chr4.1:38295355-38295374 | MS.gene08581:intron | 60.0% | |
| CTCAGTTGGCGTACAGCACG+CGG | + | chr4.1:38293481-38293500 | None:intergenic | 60.0% | |
| GCGAGGCGGTTACGAAACTC+GGG | - | chr4.1:38295356-38295375 | MS.gene08581:intron | 60.0% | |
| ! | CGGTCACCCTCGCCACTTTA+CGG | + | chr4.1:38295411-38295430 | None:intergenic | 60.0% |
| AGCCGAGGCTGCAAAACGCG+AGG | - | chr4.1:38295339-38295358 | MS.gene08581:intron | 65.0% | |
| CGAGGCTGCAAAACGCGAGG+CGG | - | chr4.1:38295342-38295361 | MS.gene08581:intron | 65.0% | |
| ! | CGCCTCGCGTTTTGCAGCCT+CGG | + | chr4.1:38295344-38295363 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 38293339 | 38295714 | 38293339 | ID=MS.gene08581 |
| chr4.1 | mRNA | 38293339 | 38295714 | 38293339 | ID=MS.gene08581.t1;Parent=MS.gene08581 |
| chr4.1 | exon | 38295704 | 38295714 | 38295704 | ID=MS.gene08581.t1.exon1;Parent=MS.gene08581.t1 |
| chr4.1 | CDS | 38295704 | 38295714 | 38295704 | ID=cds.MS.gene08581.t1;Parent=MS.gene08581.t1 |
| chr4.1 | exon | 38295432 | 38295612 | 38295432 | ID=MS.gene08581.t1.exon2;Parent=MS.gene08581.t1 |
| chr4.1 | CDS | 38295432 | 38295612 | 38295432 | ID=cds.MS.gene08581.t1;Parent=MS.gene08581.t1 |
| chr4.1 | exon | 38293339 | 38295000 | 38293339 | ID=MS.gene08581.t1.exon3;Parent=MS.gene08581.t1 |
| chr4.1 | CDS | 38293339 | 38295000 | 38293339 | ID=cds.MS.gene08581.t1;Parent=MS.gene08581.t1 |
| Gene Sequence |
| Protein sequence |
