Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09275.t1 | XP_003629603.1 | 98.9 | 174 | 2 | 0 | 1 | 174 | 1 | 174 | 1.10E-75 | 292.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09275.t1 | G7LH55 | 98.9 | 174 | 2 | 0 | 1 | 174 | 1 | 174 | 7.9e-76 | 292.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049133 | MS.gene09275 | 0.800916 | 1.15E-48 | -1.69E-46 |
| MS.gene049328 | MS.gene09275 | 0.803225 | 3.86E-49 | -1.69E-46 |
| MS.gene049397 | MS.gene09275 | 0.801185 | 1.01E-48 | -1.69E-46 |
| MS.gene049418 | MS.gene09275 | 0.883325 | 4.94E-71 | -1.69E-46 |
| MS.gene049622 | MS.gene09275 | 0.802937 | 4.43E-49 | -1.69E-46 |
| MS.gene049660 | MS.gene09275 | 0.811657 | 6.27E-51 | -1.69E-46 |
| MS.gene049989 | MS.gene09275 | 0.876213 | 1.67E-68 | -1.69E-46 |
| MS.gene050297 | MS.gene09275 | 0.883473 | 4.36E-71 | -1.69E-46 |
| MS.gene050310 | MS.gene09275 | 0.842846 | 2.01E-58 | -1.69E-46 |
| MS.gene05054 | MS.gene09275 | 0.813468 | 2.52E-51 | -1.69E-46 |
| MS.gene050589 | MS.gene09275 | 0.811764 | 5.95E-51 | -1.69E-46 |
| MS.gene050648 | MS.gene09275 | 0.810514 | 1.11E-50 | -1.69E-46 |
| MS.gene051067 | MS.gene09275 | 0.820527 | 6.54E-53 | -1.69E-46 |
| MS.gene051094 | MS.gene09275 | 0.825117 | 5.58E-54 | -1.69E-46 |
| MS.gene051263 | MS.gene09275 | 0.84989 | 2.42E-60 | -1.69E-46 |
| MS.gene051478 | MS.gene09275 | 0.864582 | 1.10E-64 | -1.69E-46 |
| MS.gene051629 | MS.gene09275 | 0.811069 | 8.42E-51 | -1.69E-46 |
| MS.gene051648 | MS.gene09275 | 0.831359 | 1.75E-55 | -1.69E-46 |
| MS.gene051671 | MS.gene09275 | 0.802244 | 6.15E-49 | -1.69E-46 |
| MS.gene052143 | MS.gene09275 | 0.827302 | 1.69E-54 | -1.69E-46 |
| MS.gene052367 | MS.gene09275 | 0.837766 | 4.28E-57 | -1.69E-46 |
| MS.gene053416 | MS.gene09275 | 0.808942 | 2.42E-50 | -1.69E-46 |
| MS.gene053417 | MS.gene09275 | 0.80855 | 2.93E-50 | -1.69E-46 |
| MS.gene053500 | MS.gene09275 | 0.801553 | 8.53E-49 | -1.69E-46 |
| MS.gene053536 | MS.gene09275 | 0.807865 | 4.10E-50 | -1.69E-46 |
| MS.gene053538 | MS.gene09275 | 0.808069 | 3.71E-50 | -1.69E-46 |
| MS.gene054643 | MS.gene09275 | 0.809788 | 1.59E-50 | -1.69E-46 |
| MS.gene055537 | MS.gene09275 | 0.8108 | 9.62E-51 | -1.69E-46 |
| MS.gene055764 | MS.gene09275 | 0.808689 | 2.74E-50 | -1.69E-46 |
| MS.gene055781 | MS.gene09275 | 0.80766 | 4.54E-50 | -1.69E-46 |
| MS.gene055957 | MS.gene09275 | 0.836282 | 1.03E-56 | -1.69E-46 |
| MS.gene056041 | MS.gene09275 | 0.83219 | 1.09E-55 | -1.69E-46 |
| MS.gene056042 | MS.gene09275 | 0.838372 | 2.99E-57 | -1.69E-46 |
| MS.gene056169 | MS.gene09275 | 0.834718 | 2.55E-56 | -1.69E-46 |
| MS.gene056228 | MS.gene09275 | 0.828455 | 8.90E-55 | -1.69E-46 |
| MS.gene056526 | MS.gene09275 | 0.829251 | 5.72E-55 | -1.69E-46 |
| MS.gene056704 | MS.gene09275 | 0.80642 | 8.30E-50 | -1.69E-46 |
| MS.gene057004 | MS.gene09275 | 0.83345 | 5.30E-56 | -1.69E-46 |
| MS.gene057483 | MS.gene09275 | 0.808052 | 3.74E-50 | -1.69E-46 |
| MS.gene058196 | MS.gene09275 | 0.8693 | 3.43E-66 | -1.69E-46 |
| MS.gene058197 | MS.gene09275 | 0.896201 | 4.63E-76 | -1.69E-46 |
| MS.gene058198 | MS.gene09275 | 0.896185 | 4.70E-76 | -1.69E-46 |
| MS.gene059163 | MS.gene09275 | 0.818897 | 1.54E-52 | -1.69E-46 |
| MS.gene059164 | MS.gene09275 | 0.81943 | 1.17E-52 | -1.69E-46 |
| MS.gene059300 | MS.gene09275 | 0.881089 | 3.21E-70 | -1.69E-46 |
| MS.gene059424 | MS.gene09275 | 0.81003 | 1.41E-50 | -1.69E-46 |
| MS.gene060473 | MS.gene09275 | 0.829107 | 6.19E-55 | -1.69E-46 |
| MS.gene060565 | MS.gene09275 | 0.850736 | 1.40E-60 | -1.69E-46 |
| MS.gene06057 | MS.gene09275 | 0.903432 | 3.49E-79 | -1.69E-46 |
| MS.gene061260 | MS.gene09275 | 0.824398 | 8.25E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09275.t1 | MTR_8g081530 | 98.851 | 174 | 2 | 0 | 1 | 174 | 1 | 174 | 6.31e-125 | 348 |
| MS.gene09275.t1 | MTR_7g080550 | 70.286 | 175 | 45 | 2 | 4 | 174 | 14 | 185 | 7.53e-81 | 238 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09275.t1 | AT5G16250 | 67.241 | 174 | 54 | 3 | 3 | 174 | 11 | 183 | 5.10e-71 | 213 |
| MS.gene09275.t1 | AT3G02640 | 61.714 | 175 | 63 | 2 | 3 | 174 | 12 | 185 | 1.47e-65 | 199 |
| MS.gene09275.t1 | AT5G36800 | 59.302 | 172 | 65 | 2 | 3 | 169 | 4 | 175 | 3.54e-61 | 187 |
| MS.gene09275.t1 | AT5G36710 | 59.302 | 172 | 65 | 2 | 3 | 169 | 4 | 175 | 3.54e-61 | 187 |
| MS.gene09275.t1 | AT5G36800 | 59.649 | 171 | 64 | 2 | 4 | 169 | 40 | 210 | 4.95e-61 | 188 |
| MS.gene09275.t1 | AT5G36710 | 59.649 | 171 | 64 | 2 | 4 | 169 | 40 | 210 | 4.95e-61 | 188 |
Find 37 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGCACCATCACCACCTTCT+TGG | 0.339448 | 4.1:+14455046 | None:intergenic |
| TCAGAATGAAGAAGATTTGA+AGG | 0.365892 | 4.1:-14455007 | MS.gene09275:CDS |
| AGAAACTGAACCTTGAAATA+TGG | 0.377833 | 4.1:+14455236 | None:intergenic |
| GTGCTAACTGCCATATTTCA+AGG | 0.401955 | 4.1:-14455246 | MS.gene09275:CDS |
| ATATTGAAGGGAAAAGGTTA+AGG | 0.429185 | 4.1:+14455385 | None:intergenic |
| ATGCTTTGTGAATGTTGTAT+TGG | 0.433769 | 4.1:+14455469 | None:intergenic |
| ATGAAGAAGATTTGAAGGAT+TGG | 0.450129 | 4.1:-14455002 | MS.gene09275:CDS |
| TGCCACATATTGAAGGGAAA+AGG | 0.478974 | 4.1:+14455379 | None:intergenic |
| AAAGGTTAAGGGAGAGAGTG+AGG | 0.489342 | 4.1:+14455397 | None:intergenic |
| TATTGAAGGGAAAAGGTTAA+GGG | 0.490541 | 4.1:+14455386 | None:intergenic |
| AAGAAGATGCCACATATTGA+AGG | 0.497178 | 4.1:+14455372 | None:intergenic |
| TGCAGAGGCGGAAACAGCTC+CGG | 0.500608 | 4.1:+14455323 | None:intergenic |
| AAGTGTTATTCTTACCAAGA+AGG | 0.514174 | 4.1:-14455060 | MS.gene09275:CDS |
| CTCCGGCGATGGTGAATGCA+TGG | 0.521359 | 4.1:+14455340 | None:intergenic |
| GGCGGAAACAGCTCCGGCGA+TGG | 0.531024 | 4.1:+14455329 | None:intergenic |
| TGCAACTTGAGGATGCAGAT+AGG | 0.536458 | 4.1:+14455424 | None:intergenic |
| CTCCATGCATTCACCATCGC+CGG | 0.537447 | 4.1:-14455342 | MS.gene09275:CDS |
| TCTTACCAAGAAGGTGGTGA+TGG | 0.567440 | 4.1:-14455051 | MS.gene09275:CDS |
| CAATAACCACTCTTACTCGT+TGG | 0.578838 | 4.1:-14455293 | MS.gene09275:CDS |
| TCCTATGTGAGGGAAGAAGA+TGG | 0.593105 | 4.1:-14455168 | MS.gene09275:CDS |
| AGAAGATGCCACATATTGAA+GGG | 0.602971 | 4.1:+14455373 | None:intergenic |
| TCGTTGGTACTCTGCACACA+TGG | 0.604168 | 4.1:-14455277 | MS.gene09275:CDS |
| TCTGTTATATTGAAACTATG+TGG | 0.612123 | 4.1:-14455144 | MS.gene09275:CDS |
| ACCATCTTCTTCCCTCACAT+AGG | 0.616023 | 4.1:+14455167 | None:intergenic |
| AGTGAGGAAGATGCAACTTG+AGG | 0.621213 | 4.1:+14455413 | None:intergenic |
| GTTATATTGAAACTATGTGG+TGG | 0.623425 | 4.1:-14455141 | MS.gene09275:CDS |
| TGTTATTCTTACCAAGAAGG+TGG | 0.624748 | 4.1:-14455057 | MS.gene09275:CDS |
| AACTAAAGCTGTAAAGACAA+TGG | 0.629500 | 4.1:+14454979 | None:intergenic |
| GGTGATGGTGCTATGAGAAG+TGG | 0.637360 | 4.1:-14455036 | MS.gene09275:CDS |
| TGGTGCTATGAGAAGTGGTA+AGG | 0.645338 | 4.1:-14455031 | MS.gene09275:CDS |
| TTGAGTTGAAATCCTATGTG+AGG | 0.659596 | 4.1:-14455179 | MS.gene09275:CDS |
| AGAGTACCAACGAGTAAGAG+TGG | 0.670822 | 4.1:+14455287 | None:intergenic |
| TTGGTACTCTGCACACATGG+TGG | 0.684738 | 4.1:-14455274 | MS.gene09275:CDS |
| TGAGTTGAAATCCTATGTGA+GGG | 0.688051 | 4.1:-14455178 | MS.gene09275:CDS |
| TATTGATGATGATGCAGAGG+CGG | 0.695458 | 4.1:+14455311 | None:intergenic |
| GGTTATTGATGATGATGCAG+AGG | 0.698569 | 4.1:+14455308 | None:intergenic |
| CGGCGATGGTGAATGCATGG+AGG | 0.710944 | 4.1:+14455343 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACTATTTTAATATTTTTGT+TGG | - | chr4.1:14455349-14455368 | MS.gene09275:CDS | 10.0% |
| !!! | TTTAATATTTTTGTTGGAGT+GGG | - | chr4.1:14455355-14455374 | MS.gene09275:CDS | 20.0% |
| !!! | TTTTAATATTTTTGTTGGAG+TGG | - | chr4.1:14455354-14455373 | MS.gene09275:CDS | 20.0% |
| ! | TCTGTTATATTGAAACTATG+TGG | - | chr4.1:14455320-14455339 | MS.gene09275:CDS | 25.0% |
| AGAAACTGAACCTTGAAATA+TGG | + | chr4.1:14455231-14455250 | None:intergenic | 30.0% | |
| ATATTGAAGGGAAAAGGTTA+AGG | + | chr4.1:14455082-14455101 | None:intergenic | 30.0% | |
| ATGAAGAAGATTTGAAGGAT+TGG | - | chr4.1:14455462-14455481 | MS.gene09275:CDS | 30.0% | |
| ATGCTTTGTGAATGTTGTAT+TGG | + | chr4.1:14454998-14455017 | None:intergenic | 30.0% | |
| GTTATATTGAAACTATGTGG+TGG | - | chr4.1:14455323-14455342 | MS.gene09275:CDS | 30.0% | |
| TATTGAAGGGAAAAGGTTAA+GGG | + | chr4.1:14455081-14455100 | None:intergenic | 30.0% | |
| TCAGAATGAAGAAGATTTGA+AGG | - | chr4.1:14455457-14455476 | MS.gene09275:CDS | 30.0% | |
| ! | AAGTGTTATTCTTACCAAGA+AGG | - | chr4.1:14455404-14455423 | MS.gene09275:CDS | 30.0% |
| AAGAAGATGCCACATATTGA+AGG | + | chr4.1:14455095-14455114 | None:intergenic | 35.0% | |
| AGAAGATGCCACATATTGAA+GGG | + | chr4.1:14455094-14455113 | None:intergenic | 35.0% | |
| TGTTATTCTTACCAAGAAGG+TGG | - | chr4.1:14455407-14455426 | MS.gene09275:CDS | 35.0% | |
| ! | AACCTTTTCCCTTCAATATG+TGG | - | chr4.1:14455083-14455102 | MS.gene09275:CDS | 35.0% |
| ! | TGAGTTGAAATCCTATGTGA+GGG | - | chr4.1:14455286-14455305 | MS.gene09275:CDS | 35.0% |
| !! | TTGAGTTGAAATCCTATGTG+AGG | - | chr4.1:14455285-14455304 | MS.gene09275:CDS | 35.0% |
| CAATAACCACTCTTACTCGT+TGG | - | chr4.1:14455171-14455190 | MS.gene09275:CDS | 40.0% | |
| GTGCTAACTGCCATATTTCA+AGG | - | chr4.1:14455218-14455237 | MS.gene09275:CDS | 40.0% | |
| TGCCACATATTGAAGGGAAA+AGG | + | chr4.1:14455088-14455107 | None:intergenic | 40.0% | |
| !! | GCTGATTTTCACAAGAACTG+AGG | - | chr4.1:14455253-14455272 | MS.gene09275:CDS | 40.0% |
| !! | GGTTATTGATGATGATGCAG+AGG | + | chr4.1:14455159-14455178 | None:intergenic | 40.0% |
| !! | TATTGATGATGATGCAGAGG+CGG | + | chr4.1:14455156-14455175 | None:intergenic | 40.0% |
| AAAGGTTAAGGGAGAGAGTG+AGG | + | chr4.1:14455070-14455089 | None:intergenic | 45.0% | |
| ACCATCTTCTTCCCTCACAT+AGG | + | chr4.1:14455300-14455319 | None:intergenic | 45.0% | |
| AGTGAGGAAGATGCAACTTG+AGG | + | chr4.1:14455054-14455073 | None:intergenic | 45.0% | |
| TCCTATGTGAGGGAAGAAGA+TGG | - | chr4.1:14455296-14455315 | MS.gene09275:CDS | 45.0% | |
| TCTTACCAAGAAGGTGGTGA+TGG | - | chr4.1:14455413-14455432 | MS.gene09275:CDS | 45.0% | |
| TGCAACTTGAGGATGCAGAT+AGG | + | chr4.1:14455043-14455062 | None:intergenic | 45.0% | |
| ! | AGAGTACCAACGAGTAAGAG+TGG | + | chr4.1:14455180-14455199 | None:intergenic | 45.0% |
| !! | TGGTGCTATGAGAAGTGGTA+AGG | - | chr4.1:14455433-14455452 | MS.gene09275:CDS | 45.0% |
| TAGCACCATCACCACCTTCT+TGG | + | chr4.1:14455421-14455440 | None:intergenic | 50.0% | |
| TCGTTGGTACTCTGCACACA+TGG | - | chr4.1:14455187-14455206 | MS.gene09275:CDS | 50.0% | |
| TTGGTACTCTGCACACATGG+TGG | - | chr4.1:14455190-14455209 | MS.gene09275:CDS | 50.0% | |
| !! | GGTGATGGTGCTATGAGAAG+TGG | - | chr4.1:14455428-14455447 | MS.gene09275:CDS | 50.0% |
| CTCCATGCATTCACCATCGC+CGG | - | chr4.1:14455122-14455141 | MS.gene09275:CDS | 55.0% | |
| CGGCGATGGTGAATGCATGG+AGG | + | chr4.1:14455124-14455143 | None:intergenic | 60.0% | |
| CTCCGGCGATGGTGAATGCA+TGG | + | chr4.1:14455127-14455146 | None:intergenic | 60.0% | |
| TGCAGAGGCGGAAACAGCTC+CGG | + | chr4.1:14455144-14455163 | None:intergenic | 60.0% | |
| GGCGGAAACAGCTCCGGCGA+TGG | + | chr4.1:14455138-14455157 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 14454981 | 14455505 | 14454981 | ID=MS.gene09275 |
| chr4.1 | mRNA | 14454981 | 14455505 | 14454981 | ID=MS.gene09275.t1;Parent=MS.gene09275 |
| chr4.1 | exon | 14454981 | 14455505 | 14454981 | ID=MS.gene09275.t1.exon1;Parent=MS.gene09275.t1 |
| chr4.1 | CDS | 14454981 | 14455505 | 14454981 | ID=cds.MS.gene09275.t1;Parent=MS.gene09275.t1 |
| Gene Sequence |
| Protein sequence |
