AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21794


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21794.t1 MTR_5g006950 95.238 168 8 0 1 168 1 168 9.26e-109 307
MS.gene21794.t1 MTR_1g103490 64.848 165 37 3 1 164 1 145 2.10e-64 194
MS.gene21794.t1 MTR_1g103470 50.595 168 61 3 1 168 1 146 6.16e-39 130
MS.gene21794.t1 MTR_7g095230 51.701 147 64 4 1 141 1 146 6.08e-38 128
MS.gene21794.t1 MTR_1g103450 53.906 128 51 3 1 127 1 121 1.98e-37 126
MS.gene21794.t1 MTR_1g071730 51.235 162 53 1 1 162 1 136 1.03e-31 111
MS.gene21794.t1 MTR_1g071730 62.264 106 40 0 1 106 1 106 3.90e-27 99.0
MS.gene21794.t1 MTR_1g097720 57.955 88 34 2 1 87 1 86 1.25e-24 91.7
MS.gene21794.t1 MTR_5g070360 31.148 183 98 6 7 162 4 185 3.75e-16 72.4
MS.gene21794.t1 MTR_3g079210 42.353 85 46 2 24 105 46 130 1.16e-15 71.6
MS.gene21794.t1 MTR_2g008560 35.606 132 73 5 7 127 4 134 1.41e-14 68.2
MS.gene21794.t1 MTR_2g008580 37.805 82 48 2 26 105 33 113 1.97e-11 60.1
MS.gene21794.t1 MTR_8g085560 31.092 119 72 3 25 134 33 150 2.54e-11 59.7
MS.gene21794.t1 MTR_5g006940 33.571 140 81 7 1 130 1 138 2.58e-11 60.1
MS.gene21794.t1 MTR_3g083760 36.486 74 46 1 25 97 28 101 7.61e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21794.t1 AT3G22600 53.757 173 67 6 5 168 2 170 1.80e-50 160
MS.gene21794.t1 AT4G14815 47.500 160 68 4 5 163 2 146 9.19e-39 129
MS.gene21794.t1 AT2G48130 40.437 183 94 5 1 168 1 183 8.58e-33 115
MS.gene21794.t1 AT2G48130 40.437 183 94 5 1 168 1 183 8.58e-33 115
MS.gene21794.t1 AT2G48130 40.437 183 94 5 1 168 1 183 8.58e-33 115
MS.gene21794.t1 AT2G48130 40.437 183 94 5 1 168 1 183 8.58e-33 115
MS.gene21794.t1 AT2G48130 40.437 183 94 5 1 168 1 183 8.58e-33 115
MS.gene21794.t1 AT2G48130 40.437 183 94 5 1 168 1 183 8.58e-33 115
MS.gene21794.t1 AT1G03103 41.520 171 83 6 6 161 1 169 1.20e-27 102
MS.gene21794.t1 AT3G43720 34.545 165 86 5 21 168 31 190 2.47e-17 75.9
MS.gene21794.t1 AT5G09370 40.000 90 50 2 26 113 26 113 6.31e-17 73.9
MS.gene21794.t1 AT5G09370 40.000 90 50 2 26 113 26 113 6.66e-17 73.2
MS.gene21794.t1 AT5G09370 40.000 90 50 2 26 113 26 113 7.42e-17 73.6
MS.gene21794.t1 AT3G43720 34.337 166 87 5 21 168 31 192 1.31e-16 73.9
MS.gene21794.t1 AT1G55260 27.389 157 107 3 1 151 44 199 1.92e-15 71.2
MS.gene21794.t1 AT2G13820 27.711 166 112 5 1 161 1 163 9.94e-15 68.6
MS.gene21794.t1 AT1G55260 28.750 160 104 5 1 151 44 202 1.10e-14 69.3
MS.gene21794.t1 AT2G13820 27.711 166 112 5 1 161 13 175 1.34e-14 68.2
MS.gene21794.t1 AT2G13820 31.034 116 74 4 1 113 1 113 7.13e-14 65.1
MS.gene21794.t1 AT4G08670 33.951 162 87 6 26 168 46 206 8.88e-14 66.6
MS.gene21794.t1 AT2G44290 32.075 159 90 6 6 148 9 165 7.12e-13 64.3
MS.gene21794.t1 AT1G36150 33.333 129 78 4 26 151 43 166 1.13e-11 61.6
MS.gene21794.t1 AT2G48140 34.884 129 66 5 8 129 4 121 1.14e-11 60.8
MS.gene21794.t1 AT3G22580 38.739 111 56 4 5 105 2 110 2.12e-11 58.5
MS.gene21794.t1 AT2G48140 35.965 114 65 4 8 114 4 116 2.64e-11 58.5
MS.gene21794.t1 AT5G64080 30.137 146 83 5 28 166 43 176 4.01e-11 58.9
MS.gene21794.t1 AT2G44300 39.726 73 43 1 25 96 32 104 4.93e-11 59.3

Find 52 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTCCGTTGGTTCTTATTT+TGG 0.199878 5.2:+1537764 MS.gene21794:CDS
CAGCAAAATGAATATGAGTT+TGG 0.304193 5.2:+1536976 MS.gene21794:CDS
AATGAATATGAGTTTGGTTT+TGG 0.324175 5.2:+1536982 MS.gene21794:CDS
GTTCCTGCTGGTGAGTCTGC+TGG 0.326138 5.2:-1537541 None:intergenic
AACGGAATTGACAGCTTGAT+TGG 0.350335 5.2:-1537750 None:intergenic
GTACCTGATGGAACTGAGTT+TGG 0.355549 5.2:-1537577 None:intergenic
AATTCCTCAACCCCGTCTTC+AGG 0.363035 5.2:+1537100 MS.gene21794:CDS
TTCCTGCTGGTGAGTCTGCT+GGG 0.376602 5.2:-1537540 None:intergenic
AGTCAGTGTAAAACTAATAA+TGG 0.381064 5.2:+1537274 MS.gene21794:CDS
GCCTATGTCAGGTTCTCGGC+GGG 0.418113 5.2:+1537167 MS.gene21794:CDS
GACTCTGCTTCTGTTCCTGC+TGG 0.439322 5.2:-1537553 None:intergenic
AGCCTTGCCTGGTGCTTGCA+AGG 0.452642 5.2:+1537234 MS.gene21794:CDS
CAGCTTGATTGGATTTCCAC+TGG 0.465066 5.2:-1537739 None:intergenic
TCCATCACCGGTTGAGGGAG+TGG 0.468250 5.2:-1537712 None:intergenic
TCAAAATCCACTCCCTCAAC+CGG 0.469317 5.2:+1537705 MS.gene21794:CDS
TTTACACTGACTGGTGGGTG+GGG 0.471462 5.2:-1537263 None:intergenic
GGATGATCCATCACCGGTTG+AGG 0.472460 5.2:-1537718 None:intergenic
GTTTGCACCTTGCAAGCACC+AGG 0.476162 5.2:-1537241 None:intergenic
TTGAGCAGCATCCTGAAGAC+GGG 0.489604 5.2:-1537111 None:intergenic
TCCACTCCCTCAACCGGTGA+TGG 0.490607 5.2:+1537711 MS.gene21794:CDS
TATAGTTAAGACAAGGTGAT+AGG 0.493717 5.2:-1537069 None:intergenic
CTATGTCAGGTTCTCGGCGG+GGG 0.499514 5.2:+1537169 MS.gene21794:CDS
ACTCAGGCTCTAGCCTTGCC+TGG 0.500358 5.2:+1537223 MS.gene21794:CDS
CACCTTGCAAGCACCAGGCA+AGG 0.514952 5.2:-1537236 None:intergenic
CTATGCCTATGTCAGGTTCT+CGG 0.519779 5.2:+1537163 MS.gene21794:CDS
ATCGAGTTGTACAAATGTCT+TGG 0.528842 5.2:+1537042 MS.gene21794:CDS
CCTTGTCTTAACTATATCAC+AGG 0.529529 5.2:+1537076 MS.gene21794:CDS
TCTCCAAACTCAGTTCCATC+AGG 0.538578 5.2:+1537574 MS.gene21794:CDS
GCATCCTGAAGACGGGGTTG+AGG 0.545184 5.2:-1537104 None:intergenic
ACAACCACTATGCCTATGTC+AGG 0.550990 5.2:+1537156 MS.gene21794:CDS
TCCACTGGATGATCCATCAC+CGG 0.555144 5.2:-1537724 None:intergenic
TGTTCAGAAAACTGAGGCAT+AGG 0.555263 5.2:-1537793 None:intergenic
AATCAAGCTGTCAATTCCGT+TGG 0.560006 5.2:+1537752 MS.gene21794:CDS
TTTGAGCAGCATCCTGAAGA+CGG 0.563609 5.2:-1537112 None:intergenic
GATGATCCATCACCGGTTGA+GGG 0.571557 5.2:-1537717 None:intergenic
TCCCCAGCAGACTCACCAGC+AGG 0.577866 5.2:+1537538 MS.gene21794:CDS
CTGCTCAAACCTTGCCAGTG+TGG 0.578926 5.2:+1537126 MS.gene21794:CDS
GTGAATTCACCACACTGGCA+AGG 0.582240 5.2:-1537135 None:intergenic
GTAATCGCAATGTTGTGCGC+TGG 0.583284 5.2:+1537007 MS.gene21794:CDS
TCCTGCTGGTGAGTCTGCTG+GGG 0.602035 5.2:-1537539 None:intergenic
TGCCTATGTCAGGTTCTCGG+CGG 0.603440 5.2:+1537166 MS.gene21794:CDS
CCCCGCCGAGAACCTGACAT+AGG 0.604243 5.2:-1537168 None:intergenic
AATCAGCATCAATCAAACTC+AGG 0.612879 5.2:+1537207 MS.gene21794:CDS
AGAACCTGACATAGGCATAG+TGG 0.618190 5.2:-1537160 None:intergenic
CCTATGTCAGGTTCTCGGCG+GGG 0.623545 5.2:+1537168 MS.gene21794:CDS
GCTGCCAAAATAAGAACCAA+CGG 0.650717 5.2:-1537768 None:intergenic
ACCGGTGATGGATCATCCAG+TGG 0.660594 5.2:+1537723 MS.gene21794:CDS
CCTGTGATATAGTTAAGACA+AGG 0.669942 5.2:-1537076 None:intergenic
CTTGTCTTAACTATATCACA+GGG 0.674749 5.2:+1537077 MS.gene21794:CDS
TGGTTGTGAATTCACCACAC+TGG 0.698930 5.2:-1537140 None:intergenic
TGAGCAGCATCCTGAAGACG+GGG 0.700847 5.2:-1537110 None:intergenic
TCAGTATGTTCAGAAAACTG+AGG 0.728921 5.2:-1537799 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATATATAGTTTAGAATAT+GGG - chr5.2:1537479-1537498 None:intergenic 10.0%
!!! GAAATATATAGTTTAGAATA+TGG - chr5.2:1537480-1537499 None:intergenic 15.0%
!! CAAATGAATTTCAACTTAAA+TGG - chr5.2:1537674-1537693 None:intergenic 20.0%
! AGTCAGTGTAAAACTAATAA+TGG + chr5.2:1537274-1537293 MS.gene21794:CDS 25.0%
! ATCTCAATGTTCATATAGTT+AGG - chr5.2:1537368-1537387 None:intergenic 25.0%
! TCTCAATGTTCATATAGTTA+GGG - chr5.2:1537367-1537386 None:intergenic 25.0%
! TTCAACTTAAATGGTTAACA+AGG - chr5.2:1537665-1537684 None:intergenic 25.0%
!!! AATGAATATGAGTTTGGTTT+TGG + chr5.2:1536982-1537001 MS.gene21794:CDS 25.0%
!!! ACTTATCTTTTTGAGTATCA+AGG - chr5.2:1537452-1537471 None:intergenic 25.0%
!!! ATTATTAGTTTTACACTGAC+TGG - chr5.2:1537275-1537294 None:intergenic 25.0%
!!! TTAAGTTGAAATTCATTTGC+AGG + chr5.2:1537675-1537694 MS.gene21794:intron 25.0%
AATGTTCATATAGTTAGGGT+TGG - chr5.2:1537363-1537382 None:intergenic 30.0%
CAGCAAAATGAATATGAGTT+TGG + chr5.2:1536976-1536995 MS.gene21794:CDS 30.0%
CTTGTCTTAACTATATCACA+GGG + chr5.2:1537077-1537096 MS.gene21794:CDS 30.0%
TATAGTTAAGACAAGGTGAT+AGG - chr5.2:1537072-1537091 None:intergenic 30.0%
!!! AATTCCGTTGGTTCTTATTT+TGG + chr5.2:1537764-1537783 MS.gene21794:CDS 30.0%
AATCAGCATCAATCAAACTC+AGG + chr5.2:1537207-1537226 MS.gene21794:CDS 35.0%
CATCAGGTACTAACATATTG+GGG + chr5.2:1537590-1537609 MS.gene21794:intron 35.0%
CCATCAGGTACTAACATATT+GGG + chr5.2:1537589-1537608 MS.gene21794:intron 35.0%
CCTGTGATATAGTTAAGACA+AGG - chr5.2:1537079-1537098 None:intergenic 35.0%
CCTTGTCTTAACTATATCAC+AGG + chr5.2:1537076-1537095 MS.gene21794:CDS 35.0%
TCCATCAGGTACTAACATAT+TGG + chr5.2:1537588-1537607 MS.gene21794:intron 35.0%
TGAAATTCATTTGCAGGAAC+CGG + chr5.2:1537681-1537700 MS.gene21794:intron 35.0%
TGATCATCCAATTTGAACCT+TGG + chr5.2:1537639-1537658 MS.gene21794:intron 35.0%
TTAAATGGTTAACAAGGCCA+AGG - chr5.2:1537659-1537678 None:intergenic 35.0%
! ATCGAGTTGTACAAATGTCT+TGG + chr5.2:1537042-1537061 MS.gene21794:CDS 35.0%
!!! ATTAGTTTTACACTGACTGG+TGG - chr5.2:1537272-1537291 None:intergenic 35.0%
!!! TTAGTTTTACACTGACTGGT+GGG - chr5.2:1537271-1537290 None:intergenic 35.0%
AACAAGGCCAAGGTTCAAAT+TGG - chr5.2:1537649-1537668 None:intergenic 40.0%
AACGGAATTGACAGCTTGAT+TGG - chr5.2:1537753-1537772 None:intergenic 40.0%
AATCAAGCTGTCAATTCCGT+TGG + chr5.2:1537752-1537771 MS.gene21794:CDS 40.0%
CCCAATATGTTAGTACCTGA+TGG - chr5.2:1537592-1537611 None:intergenic 40.0%
GCTGCCAAAATAAGAACCAA+CGG - chr5.2:1537771-1537790 None:intergenic 40.0%
ACAACCACTATGCCTATGTC+AGG + chr5.2:1537156-1537175 MS.gene21794:CDS 45.0%
AGAACCTGACATAGGCATAG+TGG - chr5.2:1537163-1537182 None:intergenic 45.0%
CAGCTTGATTGGATTTCCAC+TGG - chr5.2:1537742-1537761 None:intergenic 45.0%
CTATGCCTATGTCAGGTTCT+CGG + chr5.2:1537163-1537182 MS.gene21794:CDS 45.0%
GTACCTGATGGAACTGAGTT+TGG - chr5.2:1537580-1537599 None:intergenic 45.0%
TCAAAATCCACTCCCTCAAC+CGG + chr5.2:1537705-1537724 MS.gene21794:CDS 45.0%
TCTCCAAACTCAGTTCCATC+AGG + chr5.2:1537574-1537593 MS.gene21794:CDS 45.0%
TGGTTGTGAATTCACCACAC+TGG - chr5.2:1537143-1537162 None:intergenic 45.0%
TTTGAGCAGCATCCTGAAGA+CGG - chr5.2:1537115-1537134 None:intergenic 45.0%
!! TGAGGGAGTGGATTTTGATC+CGG - chr5.2:1537703-1537722 None:intergenic 45.0%
!! TTTTACACTGACTGGTGGGT+GGG - chr5.2:1537267-1537286 None:intergenic 45.0%
AATTCCTCAACCCCGTCTTC+AGG + chr5.2:1537100-1537119 MS.gene21794:CDS 50.0%
GTAATCGCAATGTTGTGCGC+TGG + chr5.2:1537007-1537026 MS.gene21794:CDS 50.0%
GTGAATTCACCACACTGGCA+AGG - chr5.2:1537138-1537157 None:intergenic 50.0%
TCCACTGGATGATCCATCAC+CGG - chr5.2:1537727-1537746 None:intergenic 50.0%
TTGAGCAGCATCCTGAAGAC+GGG - chr5.2:1537114-1537133 None:intergenic 50.0%
! GATGATCCATCACCGGTTGA+GGG - chr5.2:1537720-1537739 None:intergenic 50.0%
! TTTACACTGACTGGTGGGTG+GGG - chr5.2:1537266-1537285 None:intergenic 50.0%
!! GTTTTACACTGACTGGTGGG+TGG - chr5.2:1537268-1537287 None:intergenic 50.0%
CTGCTCAAACCTTGCCAGTG+TGG + chr5.2:1537126-1537145 MS.gene21794:CDS 55.0%
GACTCTGCTTCTGTTCCTGC+TGG - chr5.2:1537556-1537575 None:intergenic 55.0%
GTTTGCACCTTGCAAGCACC+AGG - chr5.2:1537244-1537263 None:intergenic 55.0%
TGAGCAGCATCCTGAAGACG+GGG - chr5.2:1537113-1537132 None:intergenic 55.0%
TGCCTATGTCAGGTTCTCGG+CGG + chr5.2:1537166-1537185 MS.gene21794:CDS 55.0%
TTCCTGCTGGTGAGTCTGCT+GGG - chr5.2:1537543-1537562 None:intergenic 55.0%
! GGATGATCCATCACCGGTTG+AGG - chr5.2:1537721-1537740 None:intergenic 55.0%
!! ACCGGTGATGGATCATCCAG+TGG + chr5.2:1537723-1537742 MS.gene21794:CDS 55.0%
AGCCTTGCCTGGTGCTTGCA+AGG + chr5.2:1537234-1537253 MS.gene21794:CDS 60.0%
CACCTTGCAAGCACCAGGCA+AGG - chr5.2:1537239-1537258 None:intergenic 60.0%
CCTATGTCAGGTTCTCGGCG+GGG + chr5.2:1537168-1537187 MS.gene21794:CDS 60.0%
CTATGTCAGGTTCTCGGCGG+GGG + chr5.2:1537169-1537188 MS.gene21794:CDS 60.0%
GCATCCTGAAGACGGGGTTG+AGG - chr5.2:1537107-1537126 None:intergenic 60.0%
GCCTATGTCAGGTTCTCGGC+GGG + chr5.2:1537167-1537186 MS.gene21794:CDS 60.0%
GTTCCTGCTGGTGAGTCTGC+TGG - chr5.2:1537544-1537563 None:intergenic 60.0%
TCCACTCCCTCAACCGGTGA+TGG + chr5.2:1537711-1537730 MS.gene21794:CDS 60.0%
TCCTGCTGGTGAGTCTGCTG+GGG - chr5.2:1537542-1537561 None:intergenic 60.0%
! TCCATCACCGGTTGAGGGAG+TGG - chr5.2:1537715-1537734 None:intergenic 60.0%
!! ACTCAGGCTCTAGCCTTGCC+TGG + chr5.2:1537223-1537242 MS.gene21794:CDS 60.0%
CCCCGCCGAGAACCTGACAT+AGG - chr5.2:1537171-1537190 None:intergenic 65.0%
TCCCCAGCAGACTCACCAGC+AGG + chr5.2:1537538-1537557 MS.gene21794:CDS 65.0%
! GGCGGGGGTGCATCGTCGCT+GGG + chr5.2:1537184-1537203 MS.gene21794:CDS 75.0%
!! CGGCGGGGGTGCATCGTCGC+TGG + chr5.2:1537183-1537202 MS.gene21794:CDS 80.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 1536968 1537812 1536968 ID=MS.gene21794
chr5.2 mRNA 1536968 1537812 1536968 ID=MS.gene21794.t1;Parent=MS.gene21794
chr5.2 exon 1536968 1537295 1536968 ID=MS.gene21794.t1.exon1;Parent=MS.gene21794.t1
chr5.2 CDS 1536968 1537295 1536968 ID=cds.MS.gene21794.t1;Parent=MS.gene21794.t1
chr5.2 exon 1537533 1537595 1537533 ID=MS.gene21794.t1.exon2;Parent=MS.gene21794.t1
chr5.2 CDS 1537533 1537595 1537533 ID=cds.MS.gene21794.t1;Parent=MS.gene21794.t1
chr5.2 exon 1537697 1537812 1537697 ID=MS.gene21794.t1.exon3;Parent=MS.gene21794.t1
chr5.2 CDS 1537697 1537812 1537697 ID=cds.MS.gene21794.t1;Parent=MS.gene21794.t1
Gene Sequence

>MS.gene21794

ATGGCACACAGCAAAATGAATATGAGTTTGGTTTTGGTTGTAATCGCAATGTTGTGCGCTGGAGCTGCTGCGCAATCGAGTTGTACAAATGTCTTGGTCAGCCTATCACCTTGTCTTAACTATATCACAGGGAATTCCTCAACCCCGTCTTCAGGATGCTGCTCAAACCTTGCCAGTGTGGTGAATTCACAACCACTATGCCTATGTCAGGTTCTCGGCGGGGGTGCATCGTCGCTGGGAATCAGCATCAATCAAACTCAGGCTCTAGCCTTGCCTGGTGCTTGCAAGGTGCAAACCCCACCCACCAGTCAGTGTAAAACTAATAATGGTAAGAGAACAAAATTTCTATTTGATTGAGTTGAGAAAAAAATCAGCTCGACAAATATGTGCATCCAACCCTAACTATATGAACATTGAGATATATACTATGCAAATATCTATCATTATACTATAGAGACAGTGAATAGAAACTATAAGAGAACCTTGATACTCAAAAAGATAAGTACAGCCCATATTCTAAACTATATATTTCTGCTAACTTACAATATATGTTTTTTGTCAACAGCTGCTTCCCCAGCAGACTCACCAGCAGGAACAGAAGCAGAGTCTCCAAACTCAGTTCCATCAGGTACTAACATATTGGGGAAGTAATTTAGTTATGCTTCAGAGCTTGATCATCCAATTTGAACCTTGGCCTTGTTAACCATTTAAGTTGAAATTCATTTGCAGGAACCGGATCAAAATCCACTCCCTCAACCGGTGATGGATCATCCAGTGGAAATCCAATCAAGCTGTCAATTCCGTTGGTTCTTATTTTGGCAGCAGCCTATGCCTCAGTTTTCTGA

Protein sequence

>MS.gene21794.t1

MAHSKMNMSLVLVVIAMLCAGAAAQSSCTNVLVSLSPCLNYITGNSSTPSSGCCSNLASVVNSQPLCLCQVLGGGASSLGISINQTQALALPGACKVQTPPTSQCKTNNAASPADSPAGTEAESPNSVPSGTGSKSTPSTGDGSSSGNPIKLSIPLVLILAAAYASVF