AlfalfaGEDB | MS.gene23251

M. sativa cultivar XinJiangDaYe / MS.gene23251

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23251.t1	XP_003622774.1	100	45	0	0	8	52	1	45	4.70E-14	86.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23251.t1	G7KY15	100.0	45	0	0	8	52	1	45	3.4e-14	86.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049491	MS.gene23251	0.800287	1.55E-48	-1.69E-46
MS.gene049758	MS.gene23251	0.872727	2.55E-67	-1.69E-46
MS.gene049915	MS.gene23251	0.825024	5.87E-54	-1.69E-46
MS.gene050099	MS.gene23251	0.827877	1.23E-54	-1.69E-46
MS.gene050301	MS.gene23251	0.833647	4.73E-56	-1.69E-46
MS.gene051471	MS.gene23251	0.82396	1.05E-53	-1.69E-46
MS.gene051626	MS.gene23251	0.805196	1.50E-49	-1.69E-46
MS.gene051662	MS.gene23251	0.847907	8.59E-60	-1.69E-46
MS.gene051663	MS.gene23251	0.848691	5.21E-60	-1.69E-46
MS.gene052078	MS.gene23251	0.810405	1.17E-50	-1.69E-46
MS.gene052178	MS.gene23251	0.802835	4.65E-49	-1.69E-46
MS.gene053836	MS.gene23251	0.864967	8.31E-65	-1.69E-46
MS.gene054184	MS.gene23251	0.814809	1.28E-51	-1.69E-46
MS.gene054185	MS.gene23251	0.822833	1.92E-53	-1.69E-46
MS.gene054667	MS.gene23251	0.824865	6.40E-54	-1.69E-46
MS.gene055133	MS.gene23251	0.855443	6.29E-62	-1.69E-46
MS.gene055277	MS.gene23251	0.828308	9.66E-55	-1.69E-46
MS.gene055390	MS.gene23251	0.825527	4.46E-54	-1.69E-46
MS.gene055422	MS.gene23251	0.865258	6.73E-65	-1.69E-46
MS.gene055428	MS.gene23251	0.814816	1.27E-51	-1.69E-46
MS.gene056069	MS.gene23251	-0.807141	5.84E-50	-1.69E-46
MS.gene056875	MS.gene23251	0.804362	2.24E-49	-1.69E-46
MS.gene057121	MS.gene23251	0.806837	6.77E-50	-1.69E-46
MS.gene057260	MS.gene23251	0.827942	1.18E-54	-1.69E-46
MS.gene057439	MS.gene23251	0.842264	2.88E-58	-1.69E-46
MS.gene057754	MS.gene23251	0.834243	3.36E-56	-1.69E-46
MS.gene057794	MS.gene23251	0.800771	1.23E-48	-1.69E-46
MS.gene058002	MS.gene23251	0.833773	4.40E-56	-1.69E-46
MS.gene05811	MS.gene23251	0.835597	1.53E-56	-1.69E-46
MS.gene058660	MS.gene23251	0.803936	2.75E-49	-1.69E-46
MS.gene058749	MS.gene23251	0.807552	4.78E-50	-1.69E-46
MS.gene058845	MS.gene23251	0.817437	3.30E-52	-1.69E-46
MS.gene05907	MS.gene23251	0.814357	1.61E-51	-1.69E-46
MS.gene059518	MS.gene23251	0.825558	4.39E-54	-1.69E-46
MS.gene059597	MS.gene23251	0.800878	1.17E-48	-1.69E-46
MS.gene05984	MS.gene23251	0.824916	6.23E-54	-1.69E-46
MS.gene059894	MS.gene23251	0.818383	2.02E-52	-1.69E-46
MS.gene06037	MS.gene23251	0.8437	1.19E-58	-1.69E-46
MS.gene060476	MS.gene23251	0.890406	1.01E-73	-1.69E-46
MS.gene061049	MS.gene23251	0.832197	1.08E-55	-1.69E-46
MS.gene061113	MS.gene23251	0.807117	5.91E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 9 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAGTAGCATGAGTACTTT+TGG	0.271602	7.3:+52209977	None:intergenic
AACTTTAGCTGCAAGAATTA+AGG	0.311122	7.3:+52209952	None:intergenic
TTCAAACTTAGTTCTCTTTG+AGG	0.370756	7.3:+52209320	None:intergenic
TTTGCAGGAATTGAAACAAA+AGG	0.418042	7.3:-52209365	MS.gene23251:intron
TCAAAGAGAACTAAGTTTGA+AGG	0.442646	7.3:-52209318	None:intergenic
CTTGCAGCTAAAGTTATACA+AGG	0.476292	7.3:-52209944	MS.gene23251:intron
TTGCAGGAATTGAAACAAAA+GGG	0.500483	7.3:-52209364	MS.gene23251:intron
TAGTTCTCTTTGAGGTTGTG+AGG	0.552834	7.3:+52209328	None:intergenic
CAGGAATTGAAACAAAAGGG+TGG	0.635486	7.3:-52209361	MS.gene23251:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GATTCTTCTTATTATTATAT+TGG	-	chr7.3:52209534-52209553	MS.gene23251:intron	15.0%
!!!	ATGTAATTTTGTAAAACTTA+TGG	+	chr7.3:52209732-52209751	None:intergenic	15.0%
!!!	TAAGTTTTACAAAATTACAT+TGG	-	chr7.3:52209732-52209751	MS.gene23251:intron	15.0%
!!	AAAAATACACCAATAAAGTA+AGG	+	chr7.3:52209658-52209677	None:intergenic	20.0%
!!	CATATTATACCTTACTTTAT+TGG	-	chr7.3:52209646-52209665	MS.gene23251:intron	20.0%
!!	TCTTTCAATTGTATATAGTT+TGG	-	chr7.3:52209562-52209581	MS.gene23251:intron	20.0%
!	AAATTAGTGCATGCTTAAAT+TGG	+	chr7.3:52209831-52209850	None:intergenic	25.0%
!	AAATTTGTAACATTCTTTGC+AGG	-	chr7.3:52209956-52209975	MS.gene23251:CDS	25.0%
!	AACAAAAAAAAAGAGGAGAA+TGG	-	chr7.3:52209322-52209341	MS.gene23251:CDS	25.0%
!	ACAAAAAAAAAGAGGAGAAT+GGG	-	chr7.3:52209323-52209342	MS.gene23251:CDS	25.0%
!	AGAATTCATTGGAACTTTAT+AGG	+	chr7.3:52209467-52209486	None:intergenic	25.0%
!	GAATCATAGTATCATTAAGT+AGG	+	chr7.3:52209437-52209456	None:intergenic	25.0%
!	GCAAAGAATGTTACAAATTT+AGG	+	chr7.3:52209956-52209975	None:intergenic	25.0%
!	TTAAAACTTCAACAAAATCG+TGG	+	chr7.3:52209760-52209779	None:intergenic	25.0%
!	TTGTAAAACTTATGGCAATT+TGG	+	chr7.3:52209724-52209743	None:intergenic	25.0%
!!	TGAAAAAAAGAAAGAGTGTA+AGG	+	chr7.3:52209505-52209524	None:intergenic	25.0%
!!!	TTTCTTTTTTTCACTTCTCT+TGG	-	chr7.3:52209512-52209531	MS.gene23251:intron	25.0%
	AACTTTAGCTGCAAGAATTA+AGG	+	chr7.3:52209387-52209406	None:intergenic	30.0%
	AGCACTTCTCTAAATTATCA+AGG	-	chr7.3:52209858-52209877	MS.gene23251:intron	30.0%
	TTGCAGGAATTGAAACAAAA+GGG	-	chr7.3:52209972-52209991	MS.gene23251:CDS	30.0%
	TTTGCAGGAATTGAAACAAA+AGG	-	chr7.3:52209971-52209990	MS.gene23251:CDS	30.0%
!	TTCAAACTTAGTTCTCTTTG+AGG	+	chr7.3:52210019-52210038	None:intergenic	30.0%
	AGAAGAAGGTGAGAATTCAT+TGG	+	chr7.3:52209478-52209497	None:intergenic	35.0%
	CTTGCAGCTAAAGTTATACA+AGG	-	chr7.3:52209392-52209411	MS.gene23251:intron	35.0%
	TTAGTGCATGCTTAAATTGG+TGG	+	chr7.3:52209828-52209847	None:intergenic	35.0%
!	AAGAGTAGCATGAGTACTTT+TGG	+	chr7.3:52209362-52209381	None:intergenic	35.0%
!	AGAGTAGCATGAGTACTTTT+GGG	+	chr7.3:52209361-52209380	None:intergenic	35.0%
	CAGGAATTGAAACAAAAGGG+TGG	-	chr7.3:52209975-52209994	MS.gene23251:CDS	40.0%
	GAGTGTAAGGACTAAGAAGA+AGG	+	chr7.3:52209492-52209511	None:intergenic	40.0%
!	TGCATGCTTAAATTGGTGGT+GGG	+	chr7.3:52209824-52209843	None:intergenic	40.0%
!!	CATGAGTACTTTTGGGAGTT+TGG	+	chr7.3:52209354-52209373	None:intergenic	40.0%
!!	TAGTTCTCTTTGAGGTTGTG+AGG	+	chr7.3:52210011-52210030	None:intergenic	40.0%
	GTGCATGCTTAAATTGGTGG+TGG	+	chr7.3:52209825-52209844	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	52209319	52210039	52209319	ID=MS.gene23251
chr7.3	mRNA	52209319	52210039	52209319	ID=MS.gene23251.t1;Parent=MS.gene23251
chr7.3	exon	52209945	52210039	52209945	ID=MS.gene23251.t1.exon1;Parent=MS.gene23251.t1
chr7.3	CDS	52209945	52210039	52209945	ID=cds.MS.gene23251.t1;Parent=MS.gene23251.t1
chr7.3	exon	52209319	52209380	52209319	ID=MS.gene23251.t1.exon2;Parent=MS.gene23251.t1
chr7.3	CDS	52209319	52209380	52209319	ID=cds.MS.gene23251.t1;Parent=MS.gene23251.t1

Gene Sequence

>MS.gene23251

CATAACAAAAAAAAAGAGGAGAATGGGTAACACCAAACTCCCAAAAGTACTCATGCTACTCTTGACCTTAATTCTTGCAGCTAAAGTTATACAAGGTCTACTCATGTCATATTGTCCTACTTAATGATACTATGATTCTTCTTTTCCTATAAAGTTCCAATGAATTCTCACCTTCTTCTTAGTCCTTACACTCTTTCTTTTTTTCACTTCTCTTGGATTCTTCTTATTATTATATTGGATGCTTCTTTCAATTGTATATAGTTTGGAGCATATATTAACTGTATTCTTAACATAAACATATATAATACTCATAATATTAGTGTTATTCATATTATACCTTACTTTATTGGTGTATTTTTCTTTATGTTATATATGATTTGCTTTAGACATTAAGTGCATTTTCCAAATTGCCATAAGTTTTACAAAATTACATTGGTGCCACGATTTTGTTGAAGTTTTAAAATGTAATATTTGCAAAAAAAAAAAATGTAATTATTCTAAACCCACCACCAATTTAAGCATGCACTAATTTCAACAAAAGCACTTCTCTAAATTATCAAGGTTGTCAAAAAACACTTTGCAGAGAAAAATAATTTAGTCTCAATTTCTTGATGCATATGCATTTTCAATGTATCCTAAATTTGTAACATTCTTTGCAGGAATTGAAACAAAAGGGTGGATATCACAATCCTCACAACCTCAAAGAGAACTAAGTTTGAAG

Protein sequence

>MS.gene23251.t1

HNKKKEENGUHQTPKSTHATLDLNSCSUSYTRNUNKRVDITILTTSKRTKFE