Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25599.t1 | XP_014494321.1 | 85.8 | 810 | 113 | 1 | 1 | 808 | 1 | 810 | 0.00E+00 | 1437.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25599.t1 | Q8L6Y1 | 73.1 | 807 | 202 | 4 | 6 | 808 | 1 | 796 | 0.0e+00 | 1231.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25599.t1 | A0A1S3TKN6 | 85.8 | 810 | 113 | 1 | 1 | 808 | 1 | 810 | 0.0e+00 | 1437.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene25599 | 0.844132 | 9.13E-59 | -1.69E-46 |
| MS.gene049208 | MS.gene25599 | 0.820502 | 6.63E-53 | -1.69E-46 |
| MS.gene049350 | MS.gene25599 | 0.812313 | 4.51E-51 | -1.69E-46 |
| MS.gene049850 | MS.gene25599 | 0.807996 | 3.85E-50 | -1.69E-46 |
| MS.gene051102 | MS.gene25599 | 0.810816 | 9.55E-51 | -1.69E-46 |
| MS.gene051247 | MS.gene25599 | 0.802151 | 6.43E-49 | -1.69E-46 |
| MS.gene051676 | MS.gene25599 | 0.813329 | 2.70E-51 | -1.69E-46 |
| MS.gene051744 | MS.gene25599 | 0.817768 | 2.78E-52 | -1.69E-46 |
| MS.gene051975 | MS.gene25599 | 0.806047 | 9.94E-50 | -1.69E-46 |
| MS.gene052307 | MS.gene25599 | 0.812685 | 3.74E-51 | -1.69E-46 |
| MS.gene052533 | MS.gene25599 | 0.801221 | 9.97E-49 | -1.69E-46 |
| MS.gene052609 | MS.gene25599 | 0.823459 | 1.37E-53 | -1.69E-46 |
| MS.gene053226 | MS.gene25599 | 0.815665 | 8.23E-52 | -1.69E-46 |
| MS.gene054139 | MS.gene25599 | 0.807651 | 4.55E-50 | -1.69E-46 |
| MS.gene054576 | MS.gene25599 | 0.809619 | 1.73E-50 | -1.69E-46 |
| MS.gene055311 | MS.gene25599 | 0.815192 | 1.05E-51 | -1.69E-46 |
| MS.gene055313 | MS.gene25599 | 0.865094 | 7.58E-65 | -1.69E-46 |
| MS.gene055314 | MS.gene25599 | 0.829594 | 4.72E-55 | -1.69E-46 |
| MS.gene055469 | MS.gene25599 | 0.802758 | 4.82E-49 | -1.69E-46 |
| MS.gene055513 | MS.gene25599 | 0.816577 | 5.15E-52 | -1.69E-46 |
| MS.gene055603 | MS.gene25599 | 0.80796 | 3.92E-50 | -1.69E-46 |
| MS.gene055864 | MS.gene25599 | 0.807257 | 5.52E-50 | -1.69E-46 |
| MS.gene057026 | MS.gene25599 | 0.806808 | 6.87E-50 | -1.69E-46 |
| MS.gene057360 | MS.gene25599 | 0.85137 | 9.27E-61 | -1.69E-46 |
| MS.gene057915 | MS.gene25599 | 0.842844 | 2.02E-58 | -1.69E-46 |
| MS.gene058289 | MS.gene25599 | 0.807769 | 4.30E-50 | -1.69E-46 |
| MS.gene058292 | MS.gene25599 | 0.810073 | 1.38E-50 | -1.69E-46 |
| MS.gene058639 | MS.gene25599 | 0.820071 | 8.33E-53 | -1.69E-46 |
| MS.gene058668 | MS.gene25599 | 0.806083 | 9.77E-50 | -1.69E-46 |
| MS.gene05897 | MS.gene25599 | 0.825141 | 5.51E-54 | -1.69E-46 |
| MS.gene059725 | MS.gene25599 | 0.830562 | 2.74E-55 | -1.69E-46 |
| MS.gene060191 | MS.gene25599 | 0.808068 | 3.71E-50 | -1.69E-46 |
| MS.gene060933 | MS.gene25599 | 0.811923 | 5.49E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene050290 | MS.gene25599 | PPI |
| MS.gene25599 | MS.gene06859 | PPI |
| MS.gene013534 | MS.gene25599 | PPI |
| MS.gene24706 | MS.gene25599 | PPI |
| MS.gene06645 | MS.gene25599 | PPI |
| MS.gene25599 | MS.gene034846 | PPI |
| MS.gene25599 | MS.gene056896 | PPI |
| MS.gene007189 | MS.gene25599 | PPI |
| MS.gene057206 | MS.gene25599 | PPI |
| MS.gene25599 | MS.gene050252 | PPI |
| MS.gene25599 | MS.gene81765 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25599.t1 | MTR_2g081180 | 79.752 | 805 | 158 | 2 | 6 | 808 | 1 | 802 | 0.0 | 1375 |
| MS.gene25599.t1 | MTR_2g081180 | 78.882 | 805 | 151 | 3 | 6 | 808 | 1 | 788 | 0.0 | 1349 |
| MS.gene25599.t1 | MTR_2g081180 | 78.012 | 805 | 154 | 3 | 6 | 808 | 1 | 784 | 0.0 | 1335 |
| MS.gene25599.t1 | MTR_2g081180 | 79.401 | 568 | 112 | 2 | 243 | 808 | 4 | 568 | 0.0 | 956 |
| MS.gene25599.t1 | MTR_2g081180 | 79.135 | 532 | 106 | 2 | 279 | 808 | 1 | 529 | 0.0 | 894 |
| MS.gene25599.t1 | MTR_8g464880 | 88.333 | 120 | 9 | 1 | 632 | 751 | 1 | 115 | 2.32e-64 | 211 |
| MS.gene25599.t1 | MTR_4g077475 | 63.235 | 68 | 25 | 0 | 743 | 810 | 64 | 131 | 5.80e-23 | 95.5 |
| MS.gene25599.t1 | MTR_4g077475 | 63.235 | 68 | 24 | 1 | 552 | 619 | 2 | 68 | 1.27e-18 | 83.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25599.t1 | AT3G20630 | 73.110 | 807 | 202 | 4 | 6 | 808 | 1 | 796 | 0.0 | 1244 |
Find 180 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCAATCAAAATTTGTATTT+TGG | 0.104884 | 8.2:-44150946 | None:intergenic |
| TTGGCGCATGGTCTACTTTC+TGG | 0.199366 | 8.2:+44152116 | MS.gene25599:CDS |
| CTATGAGTTTCTTCATATTC+AGG | 0.242690 | 8.2:-44148840 | None:intergenic |
| AACACAGGATATCGACTTAT+TGG | 0.246392 | 8.2:+44159293 | MS.gene25599:intron |
| ATTTGTTGACAGGACAAAAT+TGG | 0.248332 | 8.2:+44152097 | MS.gene25599:intron |
| TCAGACACTGCATCCATTTC+CGG | 0.278760 | 8.2:+44158540 | MS.gene25599:CDS |
| GAACCATGGCCTTCAGAAAT+AGG | 0.283309 | 8.2:-44157950 | None:intergenic |
| AATAGTGTTGGTTTCTTAGA+AGG | 0.292223 | 8.2:-44147254 | None:intergenic |
| AGCGTTGTTATTCCTCCATC+TGG | 0.297440 | 8.2:+44149185 | MS.gene25599:CDS |
| TTCTTAGAAGGCCTATCTTC+TGG | 0.304039 | 8.2:-44147242 | None:intergenic |
| ATGTTTCGTGTAGGTCTTGA+TGG | 0.327495 | 8.2:+44148798 | MS.gene25599:intron |
| CATAAAGCAGACAAAGAAAT+TGG | 0.327783 | 8.2:+44147217 | MS.gene25599:CDS |
| TCCAAATTTAGCAAAACATT+TGG | 0.328071 | 8.2:+44150258 | MS.gene25599:CDS |
| ATTACAGCTTTGAACATATT+CGG | 0.328753 | 8.2:-44152446 | None:intergenic |
| TGTTAATCCTCCCAAGGAAA+TGG | 0.328991 | 8.2:+44159421 | MS.gene25599:CDS |
| TAGAGAGACAATTTCTTCAT+TGG | 0.337556 | 8.2:-44157705 | None:intergenic |
| CAGGCCAGTGTCACCGGAAA+TGG | 0.339385 | 8.2:-44158553 | None:intergenic |
| GATAGAATTTCATGTTCATC+TGG | 0.348308 | 8.2:+44153358 | MS.gene25599:CDS |
| GTGATAAGAAAGACAATCTT+TGG | 0.354423 | 8.2:+44149225 | MS.gene25599:CDS |
| TGTCTCTCTACTTGTTTCCA+TGG | 0.356143 | 8.2:+44157718 | MS.gene25599:CDS |
| AAAATGCGATTGGTAGGTTC+AGG | 0.362949 | 8.2:-44146682 | None:intergenic |
| GATTGGTCAACATTGGAAAT+AGG | 0.374998 | 8.2:+44151026 | MS.gene25599:CDS |
| CCAAACTTGAAACTCCTTGC+AGG | 0.390469 | 8.2:-44153328 | None:intergenic |
| TGGCGCATGGTCTACTTTCT+GGG | 0.390969 | 8.2:+44152117 | MS.gene25599:CDS |
| ATGTGTAACACCAGGTAATC+AGG | 0.392442 | 8.2:-44155130 | None:intergenic |
| TGACTGATATCTATGATATC+AGG | 0.393855 | 8.2:-44156677 | None:intergenic |
| TGCTTCGTTCCAATCTCTCT+AGG | 0.399630 | 8.2:+44146650 | MS.gene25599:CDS |
| GGAAAGTTACATACAATAGA+AGG | 0.402363 | 8.2:+44153380 | MS.gene25599:CDS |
| CTCTGCTTTGAGTTTGTGAA+AGG | 0.405467 | 8.2:-44153560 | None:intergenic |
| CTTTCTTGATCGAGTTGAAC+GGG | 0.409811 | 8.2:+44153279 | MS.gene25599:CDS |
| GATTTGTTATGGAGGAAGGA+TGG | 0.414081 | 8.2:+44155158 | MS.gene25599:CDS |
| TGCCGAATAGATGAAAATTC+AGG | 0.414367 | 8.2:-44152482 | None:intergenic |
| ACACATGCGAAGATTTGTTA+TGG | 0.414720 | 8.2:+44155147 | MS.gene25599:CDS |
| TGGCACTATTACTGCTGATC+TGG | 0.415852 | 8.2:+44149364 | MS.gene25599:CDS |
| TTCTAAGAAACCAACACTAT+TGG | 0.417301 | 8.2:+44147256 | MS.gene25599:CDS |
| GTCTCTCTACTTGTTTCCAT+GGG | 0.420502 | 8.2:+44157719 | MS.gene25599:CDS |
| TGAGGCATCAGTCTCCAGTA+TGG | 0.422118 | 8.2:+44158506 | MS.gene25599:CDS |
| TTCTGGGAAATACTCTGTTC+CGG | 0.425141 | 8.2:+44152133 | MS.gene25599:CDS |
| TAAGGAGCAACTTGCAGCTT+GGG | 0.425340 | 8.2:+44149106 | MS.gene25599:CDS |
| ATACTCTGTTCCGGCATTCA+AGG | 0.426379 | 8.2:+44152142 | MS.gene25599:CDS |
| CCTGCAAGGAGTTTCAAGTT+TGG | 0.431104 | 8.2:+44153328 | MS.gene25599:CDS |
| CAAAATAATTTAGGTAAGGC+TGG | 0.434814 | 8.2:+44149046 | MS.gene25599:intron |
| GTTCCAATCTCTCTAGGGTT+CGG | 0.435089 | 8.2:+44146656 | MS.gene25599:CDS |
| TTGTTTGTTGCTTCATGCAA+TGG | 0.437604 | 8.2:-44153430 | None:intergenic |
| CAAATGCCTTCTCTAGACTT+TGG | 0.438288 | 8.2:-44151282 | None:intergenic |
| GTTGATCAGCTAATTTCGTT+CGG | 0.445072 | 8.2:+44158004 | MS.gene25599:CDS |
| GACCTCATTTCCTGATTACC+TGG | 0.445780 | 8.2:+44155120 | MS.gene25599:CDS |
| GTTAATCCTCCCAAGGAAAT+GGG | 0.449545 | 8.2:+44159422 | MS.gene25599:CDS |
| CGAGTTGAACGGGCTAATGA+TGG | 0.453581 | 8.2:+44153289 | MS.gene25599:CDS |
| AGTTTATTCTGTAGGACTGC+AGG | 0.454873 | 8.2:+44155094 | MS.gene25599:intron |
| AGAAATTGGTTCCAGAAGAT+AGG | 0.456451 | 8.2:+44147231 | MS.gene25599:CDS |
| CTCCTTACGTTCAGCACCAT+CGG | 0.457531 | 8.2:-44149090 | None:intergenic |
| GATCCGAACCCTAGAGAGAT+TGG | 0.459812 | 8.2:-44146659 | None:intergenic |
| TGTTTGTTGCTTCATGCAAT+GGG | 0.461560 | 8.2:-44153429 | None:intergenic |
| ATCAGGCAGGCCAGTGTCAC+CGG | 0.462555 | 8.2:-44158559 | None:intergenic |
| ATACAGATAACCCATTTCCT+TGG | 0.463944 | 8.2:-44159432 | None:intergenic |
| TACAGATAACCCATTTCCTT+GGG | 0.464634 | 8.2:-44159431 | None:intergenic |
| AACTTACTCCCTCCTCCATC+AGG | 0.464709 | 8.2:-44158576 | None:intergenic |
| TGGACCTGGTTATACAGGAT+TGG | 0.474300 | 8.2:+44151009 | MS.gene25599:CDS |
| TGCTTTATGCGCAAATAGAT+CGG | 0.477786 | 8.2:-44147203 | None:intergenic |
| CAAGCTTCTAAAGGCACCCT+TGG | 0.478820 | 8.2:-44153706 | None:intergenic |
| ACTGGCCTGCCTGATGGAGG+AGG | 0.483489 | 8.2:+44158567 | MS.gene25599:CDS |
| GACTGGATGCGGCATTGACT+TGG | 0.484260 | 8.2:+44157792 | MS.gene25599:CDS |
| AGGTCTACAGTTGGATCAGC+AGG | 0.486223 | 8.2:-44151313 | None:intergenic |
| AGATGAAAATTCAGGATGGC+TGG | 0.486516 | 8.2:-44152474 | None:intergenic |
| ATTAGAAACAAGAAGGAATA+CGG | 0.486941 | 8.2:+44152423 | MS.gene25599:intron |
| ACACTTACAACTGCATATTC+AGG | 0.488171 | 8.2:-44151333 | None:intergenic |
| GAGCAGTTAGGTACCTTCTC+TGG | 0.489496 | 8.2:-44148915 | None:intergenic |
| ATTTGTTATGGAGGAAGGAT+GGG | 0.493649 | 8.2:+44155159 | MS.gene25599:CDS |
| TTTGCAAGGCAAGCTTCTAA+AGG | 0.494194 | 8.2:-44153715 | None:intergenic |
| GGTGACACTGGCCTGCCTGA+TGG | 0.494563 | 8.2:+44158561 | MS.gene25599:CDS |
| ACTGATGGAATGATTCTTTG+TGG | 0.494947 | 8.2:+44149257 | MS.gene25599:CDS |
| ACATTGAGAAAGCGACAGAT+TGG | 0.495965 | 8.2:+44158469 | MS.gene25599:CDS |
| ATTGACATTCCTATTTCTGA+AGG | 0.497250 | 8.2:+44157941 | MS.gene25599:CDS |
| CTGATGGAATGATTCTTTGT+GGG | 0.499028 | 8.2:+44149258 | MS.gene25599:CDS |
| CGAAGATTTGTTATGGAGGA+AGG | 0.499090 | 8.2:+44155154 | MS.gene25599:CDS |
| TCGGAAAGGATTACGTTGGT+TGG | 0.499687 | 8.2:+44147159 | MS.gene25599:CDS |
| TCCAAGACACTATCATCCTC+TGG | 0.503510 | 8.2:-44150235 | None:intergenic |
| ATCAAATGTTGGACTGGATG+CGG | 0.504815 | 8.2:+44157781 | MS.gene25599:CDS |
| GCTATAAATACATCAAATGT+TGG | 0.506211 | 8.2:+44157770 | MS.gene25599:CDS |
| TGCATATTCAGGTCTACAGT+TGG | 0.508855 | 8.2:-44151322 | None:intergenic |
| TATGCGCAGCAAAGGTCGCC+AGG | 0.508888 | 8.2:+44156700 | MS.gene25599:CDS |
| TAAAAGTCGTGTATATCCTC+TGG | 0.510008 | 8.2:-44153748 | None:intergenic |
| CACCCTCTTGCTGTAAAGCT+TGG | 0.510174 | 8.2:+44149344 | MS.gene25599:CDS |
| CAGTTTCACAAGGATGATCC+AGG | 0.510435 | 8.2:+44157818 | MS.gene25599:CDS |
| AAATACATCAAATGTTGGAC+TGG | 0.510805 | 8.2:+44157775 | MS.gene25599:CDS |
| TGGAATCGAATTCAAGAAAG+TGG | 0.512721 | 8.2:+44150965 | MS.gene25599:CDS |
| TGTTTCGTGTAGGTCTTGAT+GGG | 0.514346 | 8.2:+44148799 | MS.gene25599:intron |
| TCCAGAGGATGATAGTGTCT+TGG | 0.518327 | 8.2:+44150234 | MS.gene25599:CDS |
| TATACAGGATTGGTCAACAT+TGG | 0.520849 | 8.2:+44151019 | MS.gene25599:CDS |
| AGCCGATGGTGCTGAACGTA+AGG | 0.522162 | 8.2:+44149088 | MS.gene25599:CDS |
| GCTTCGTTCCAATCTCTCTA+GGG | 0.522341 | 8.2:+44146651 | MS.gene25599:CDS |
| TTTCAATGACAACAAGGTTG+GGG | 0.523089 | 8.2:+44159394 | MS.gene25599:CDS |
| GGTTGGAATTATGACAAGAC+TGG | 0.523865 | 8.2:+44147176 | MS.gene25599:CDS |
| GCGATGAGATTGTGCGTCCA+AGG | 0.524499 | 8.2:+44153689 | MS.gene25599:CDS |
| GATGAATTTCAGAGGTCAGA+AGG | 0.527016 | 8.2:+44147101 | MS.gene25599:intron |
| TGATGTGACAGTATCCATAC+TGG | 0.528032 | 8.2:-44158520 | None:intergenic |
| GACGATGATAACCGAAGCGT+TGG | 0.528314 | 8.2:+44146624 | None:intergenic |
| CGCAGCAAAGGTCGCCAGGA+TGG | 0.529367 | 8.2:+44156704 | MS.gene25599:CDS |
| CAACAAGATCATTCATATCA+CGG | 0.532315 | 8.2:+44151167 | MS.gene25599:CDS |
| TACTCCCTCCTCCATCAGGC+AGG | 0.532645 | 8.2:-44158572 | None:intergenic |
| ATTCCTATTTCTGAAGGCCA+TGG | 0.532648 | 8.2:+44157947 | MS.gene25599:CDS |
| CTTTGGCTAAATCTAACTGA+TGG | 0.536278 | 8.2:+44149242 | MS.gene25599:CDS |
| ACTTTCTTGATCGAGTTGAA+CGG | 0.542582 | 8.2:+44153278 | MS.gene25599:CDS |
| GACACTGGCCTGCCTGATGG+AGG | 0.543208 | 8.2:+44158564 | MS.gene25599:CDS |
| GACATACATACCTTGAATGC+CGG | 0.545190 | 8.2:-44152152 | None:intergenic |
| TTCTCTGGCAATTCGACAGA+AGG | 0.546298 | 8.2:-44148900 | None:intergenic |
| AAATGCGATTGGTAGGTTCA+GGG | 0.549022 | 8.2:-44146681 | None:intergenic |
| GCATCCATTTCCGGTGACAC+TGG | 0.549195 | 8.2:+44158549 | MS.gene25599:CDS |
| GTAAGGAGCAACTTGCAGCT+TGG | 0.549619 | 8.2:+44149105 | MS.gene25599:CDS |
| ACGAAGCAAATCCAACGCTT+CGG | 0.550712 | 8.2:-44146635 | None:intergenic |
| AGAGAAACTTGCAGAAGGAA+AGG | 0.551882 | 8.2:+44153579 | MS.gene25599:CDS |
| ATAGAATTTCATGTTCATCT+GGG | 0.555484 | 8.2:+44153359 | MS.gene25599:CDS |
| TTCGTGCTTGAAAATGCGAT+TGG | 0.555490 | 8.2:-44146692 | None:intergenic |
| TTCAAACATTAGAAACAAGA+AGG | 0.556304 | 8.2:+44152416 | MS.gene25599:intron |
| ATTGTCTTTCTTATCACACT+TGG | 0.558797 | 8.2:-44149219 | None:intergenic |
| GTAGCAGTTCTTCACCATCC+TGG | 0.558986 | 8.2:-44156718 | None:intergenic |
| GCGTTGTTATTCCTCCATCT+GGG | 0.565412 | 8.2:+44149186 | MS.gene25599:CDS |
| ATCAGTCATATGCGCAGCAA+AGG | 0.565733 | 8.2:+44156692 | MS.gene25599:CDS |
| TTGGCACATTTCCACCCAGA+TGG | 0.567451 | 8.2:-44149200 | None:intergenic |
| CGATGAGATTGTGCGTCCAA+GGG | 0.570277 | 8.2:+44153690 | MS.gene25599:CDS |
| TAATGATGGGAAAACTGAGC+TGG | 0.572138 | 8.2:+44153303 | MS.gene25599:CDS |
| AGATAACCCATTTCCTTGGG+AGG | 0.572510 | 8.2:-44159428 | None:intergenic |
| ACTGCTGATCTGGAAGCAGC+AGG | 0.573313 | 8.2:+44149374 | MS.gene25599:CDS |
| TTCTGTCGAATTGCCAGAGA+AGG | 0.575999 | 8.2:+44148902 | MS.gene25599:CDS |
| TCTTACAGCGGTCGTAGTTG+AGG | 0.577916 | 8.2:-44152278 | None:intergenic |
| GGCTTCTGTTAATCCTCCCA+AGG | 0.578435 | 8.2:+44159415 | MS.gene25599:CDS |
| CTGGCCTGCCTGATGGAGGA+GGG | 0.578722 | 8.2:+44158568 | MS.gene25599:CDS |
| ACAATTTCTTCATTGGCCGA+AGG | 0.578908 | 8.2:-44157698 | None:intergenic |
| CACCAGGTAATCAGGAAATG+AGG | 0.581945 | 8.2:-44155122 | None:intergenic |
| GTGAAGAACTGCTACCTGAT+GGG | 0.584775 | 8.2:+44156726 | MS.gene25599:CDS |
| TTATAGCAGCTTTCTGACAA+CGG | 0.586766 | 8.2:-44157754 | None:intergenic |
| GTTTCGTGTAGGTCTTGATG+GGG | 0.588680 | 8.2:+44148800 | MS.gene25599:intron |
| ATACTGGAGACTGATGCCTC+AGG | 0.590591 | 8.2:-44158504 | None:intergenic |
| GTGCCAAGCTTTACAGCAAG+AGG | 0.591223 | 8.2:-44149347 | None:intergenic |
| GAGTTGAACGGGCTAATGAT+GGG | 0.592153 | 8.2:+44153290 | MS.gene25599:CDS |
| GGTGAAGAACTGCTACCTGA+TGG | 0.592381 | 8.2:+44156725 | MS.gene25599:CDS |
| TGAAGAACTGCTACCTGATG+GGG | 0.593296 | 8.2:+44156727 | MS.gene25599:CDS |
| TGCTTGAAAATGCGATTGGT+AGG | 0.595745 | 8.2:-44146688 | None:intergenic |
| AATTTCAGAGGTCAGAAGGT+GGG | 0.598431 | 8.2:+44147105 | MS.gene25599:intron |
| GTTATCTGTATTTCTTCGAG+AGG | 0.598528 | 8.2:+44159444 | MS.gene25599:CDS |
| GACACTATCATCCTCTGGAT+AGG | 0.601245 | 8.2:-44150230 | None:intergenic |
| TTGGTCAAGCTCTCTTTCAG+CGG | 0.603928 | 8.2:-44150927 | None:intergenic |
| CAACTCGATCAAGAAAGTGT+AGG | 0.609529 | 8.2:-44153273 | None:intergenic |
| ATACCTACAGCCAATAGTGT+TGG | 0.609740 | 8.2:-44147266 | None:intergenic |
| GCACATTTCCACCCAGATGG+AGG | 0.611161 | 8.2:-44149197 | None:intergenic |
| CTGTTGTAAACTCACTGCAT+AGG | 0.613715 | 8.2:-44149153 | None:intergenic |
| GCTTTCGGAAAGGATTACGT+TGG | 0.616166 | 8.2:+44147155 | MS.gene25599:CDS |
| TTGCGTTTCTTACGACACTC+CGG | 0.616701 | 8.2:+44146717 | MS.gene25599:CDS |
| TTGCATGAAGCAACAAACAA+AGG | 0.616933 | 8.2:+44153433 | MS.gene25599:CDS |
| AAACTGAGCTGGATCCTGCA+AGG | 0.617385 | 8.2:+44153314 | MS.gene25599:CDS |
| GACTTGGTTACAGTTTCACA+AGG | 0.617561 | 8.2:+44157808 | MS.gene25599:CDS |
| GAATTTCAGAGGTCAGAAGG+TGG | 0.618238 | 8.2:+44147104 | MS.gene25599:intron |
| ACGGAGATGATCAAAGCCCA+TGG | 0.619505 | 8.2:-44157735 | None:intergenic |
| GACAGGACAAAATTGGCGCA+TGG | 0.621055 | 8.2:+44152104 | MS.gene25599:intron |
| CTTGAACCAAAGTCTAGAGA+AGG | 0.624274 | 8.2:+44151276 | MS.gene25599:CDS |
| GATGCAATCTTACTAGCCGA+TGG | 0.625399 | 8.2:+44149074 | MS.gene25599:CDS |
| TTGTTATTCCTCCATCTGGG+TGG | 0.625839 | 8.2:+44149189 | MS.gene25599:CDS |
| CTGGAGCAGAGAAGTTTGCA+AGG | 0.626937 | 8.2:-44153729 | None:intergenic |
| ATTGGAGTTGTTAGCCACAG+TGG | 0.628087 | 8.2:+44159311 | MS.gene25599:CDS |
| TTGTCAAAGCTTCAGAACCA+TGG | 0.631887 | 8.2:-44157964 | None:intergenic |
| TGCAAACTTCTCTGCTCCAG+AGG | 0.633061 | 8.2:+44153732 | MS.gene25599:CDS |
| CATGCGAAGATTTGTTATGG+AGG | 0.633316 | 8.2:+44155150 | MS.gene25599:CDS |
| AAACCAACACTATTGGCTGT+AGG | 0.633486 | 8.2:+44147263 | MS.gene25599:CDS |
| TTGACCAATCCTGTATAACC+AGG | 0.634222 | 8.2:-44151013 | None:intergenic |
| TCTCTGGCAATTCGACAGAA+GGG | 0.635011 | 8.2:-44148899 | None:intergenic |
| AAAGCAGAGAAACTTGCAGA+AGG | 0.639104 | 8.2:+44153574 | MS.gene25599:CDS |
| GGAGCAACTTGCAGCTTGGG+CGG | 0.640605 | 8.2:+44149109 | MS.gene25599:CDS |
| ATGGAATGATTCTTTGTGGG+AGG | 0.642009 | 8.2:+44149261 | MS.gene25599:CDS |
| TTGGATATTTAGCAATCCTG+AGG | 0.649412 | 8.2:+44158488 | MS.gene25599:CDS |
| TTTCGTGTAGGTCTTGATGG+GGG | 0.655544 | 8.2:+44148801 | MS.gene25599:intron |
| AATGAAAGAGACACATACTC+AGG | 0.656070 | 8.2:-44148876 | None:intergenic |
| TGCCAAGCTTTACAGCAAGA+GGG | 0.664548 | 8.2:-44149346 | None:intergenic |
| ATCTTCGCATGTGTAACACC+AGG | 0.671341 | 8.2:-44155138 | None:intergenic |
| AGTGGAACCTCTACGCTGTG+TGG | 0.697486 | 8.2:+44159329 | MS.gene25599:CDS |
| GAATAGATGAAAATTCAGGA+TGG | 0.699038 | 8.2:-44152478 | None:intergenic |
| GAAGAACTGCTACCTGATGG+GGG | 0.708896 | 8.2:+44156728 | MS.gene25599:CDS |
| AGACGTTTACTCCTATCCAG+AGG | 0.709737 | 8.2:+44150219 | MS.gene25599:intron |
| GTAGTGACCACACAGCGTAG+AGG | 0.713372 | 8.2:-44159336 | None:intergenic |
| ACAGCGTAGAGGTTCCACTG+TGG | 0.816807 | 8.2:-44159325 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 44146628 | 44159478 | 44146628 | ID=MS.gene25599 |
| chr8.2 | mRNA | 44146628 | 44159478 | 44146628 | ID=MS.gene25599.t1;Parent=MS.gene25599 |
| chr8.2 | exon | 44146628 | 44146738 | 44146628 | ID=MS.gene25599.t1.exon1;Parent=MS.gene25599.t1 |
| chr8.2 | CDS | 44146628 | 44146738 | 44146628 | ID=cds.MS.gene25599.t1;Parent=MS.gene25599.t1 |
| chr8.2 | exon | 44147113 | 44147284 | 44147113 | ID=MS.gene25599.t1.exon2;Parent=MS.gene25599.t1 |
| chr8.2 | CDS | 44147113 | 44147284 | 44147113 | ID=cds.MS.gene25599.t1;Parent=MS.gene25599.t1 |
| chr8.2 | exon | 44148811 | 44148923 | 44148811 | ID=MS.gene25599.t1.exon3;Parent=MS.gene25599.t1 |
| chr8.2 | CDS | 44148811 | 44148923 | 44148811 | ID=cds.MS.gene25599.t1;Parent=MS.gene25599.t1 |
| chr8.2 | exon | 44149059 | 44149395 | 44149059 | ID=MS.gene25599.t1.exon4;Parent=MS.gene25599.t1 |
| chr8.2 | CDS | 44149059 | 44149395 | 44149059 | ID=cds.MS.gene25599.t1;Parent=MS.gene25599.t1 |
| chr8.2 | exon | 44150221 | 44150315 | 44150221 | ID=MS.gene25599.t1.exon5;Parent=MS.gene25599.t1 |
| chr8.2 | CDS | 44150221 | 44150315 | 44150221 | ID=cds.MS.gene25599.t1;Parent=MS.gene25599.t1 |
| chr8.2 | exon | 44150914 | 44151047 | 44150914 | ID=MS.gene25599.t1.exon6;Parent=MS.gene25599.t1 |
| chr8.2 | CDS | 44150914 | 44151047 | 44150914 | ID=cds.MS.gene25599.t1;Parent=MS.gene25599.t1 |
| chr8.2 | exon | 44151132 | 44151188 | 44151132 | ID=MS.gene25599.t1.exon7;Parent=MS.gene25599.t1 |
| chr8.2 | CDS | 44151132 | 44151188 | 44151132 | ID=cds.MS.gene25599.t1;Parent=MS.gene25599.t1 |
| chr8.2 | exon | 44151270 | 44151347 | 44151270 | ID=MS.gene25599.t1.exon8;Parent=MS.gene25599.t1 |
| chr8.2 | CDS | 44151270 | 44151347 | 44151270 | ID=cds.MS.gene25599.t1;Parent=MS.gene25599.t1 |
| chr8.2 | exon | 44152109 | 44152163 | 44152109 | ID=MS.gene25599.t1.exon9;Parent=MS.gene25599.t1 |
| chr8.2 | CDS | 44152109 | 44152163 | 44152109 | ID=cds.MS.gene25599.t1;Parent=MS.gene25599.t1 |
| chr8.2 | exon | 44152253 | 44152294 | 44152253 | ID=MS.gene25599.t1.exon10;Parent=MS.gene25599.t1 |
| chr8.2 | CDS | 44152253 | 44152294 | 44152253 | ID=cds.MS.gene25599.t1;Parent=MS.gene25599.t1 |
| chr8.2 | exon | 44152428 | 44152508 | 44152428 | ID=MS.gene25599.t1.exon11;Parent=MS.gene25599.t1 |
| chr8.2 | CDS | 44152428 | 44152508 | 44152428 | ID=cds.MS.gene25599.t1;Parent=MS.gene25599.t1 |
| chr8.2 | exon | 44153256 | 44153454 | 44153256 | ID=MS.gene25599.t1.exon12;Parent=MS.gene25599.t1 |
| chr8.2 | CDS | 44153256 | 44153454 | 44153256 | ID=cds.MS.gene25599.t1;Parent=MS.gene25599.t1 |
| chr8.2 | exon | 44153548 | 44153605 | 44153548 | ID=MS.gene25599.t1.exon13;Parent=MS.gene25599.t1 |
| chr8.2 | CDS | 44153548 | 44153605 | 44153548 | ID=cds.MS.gene25599.t1;Parent=MS.gene25599.t1 |
| chr8.2 | exon | 44153684 | 44153806 | 44153684 | ID=MS.gene25599.t1.exon14;Parent=MS.gene25599.t1 |
| chr8.2 | CDS | 44153684 | 44153806 | 44153684 | ID=cds.MS.gene25599.t1;Parent=MS.gene25599.t1 |
| chr8.2 | exon | 44155108 | 44155196 | 44155108 | ID=MS.gene25599.t1.exon15;Parent=MS.gene25599.t1 |
| chr8.2 | CDS | 44155108 | 44155196 | 44155108 | ID=cds.MS.gene25599.t1;Parent=MS.gene25599.t1 |
| chr8.2 | exon | 44156660 | 44156749 | 44156660 | ID=MS.gene25599.t1.exon16;Parent=MS.gene25599.t1 |
| chr8.2 | CDS | 44156660 | 44156749 | 44156660 | ID=cds.MS.gene25599.t1;Parent=MS.gene25599.t1 |
| chr8.2 | exon | 44157678 | 44157839 | 44157678 | ID=MS.gene25599.t1.exon17;Parent=MS.gene25599.t1 |
| chr8.2 | CDS | 44157678 | 44157839 | 44157678 | ID=cds.MS.gene25599.t1;Parent=MS.gene25599.t1 |
| chr8.2 | exon | 44157939 | 44158066 | 44157939 | ID=MS.gene25599.t1.exon18;Parent=MS.gene25599.t1 |
| chr8.2 | CDS | 44157939 | 44158066 | 44157939 | ID=cds.MS.gene25599.t1;Parent=MS.gene25599.t1 |
| chr8.2 | exon | 44158462 | 44158591 | 44158462 | ID=MS.gene25599.t1.exon19;Parent=MS.gene25599.t1 |
| chr8.2 | CDS | 44158462 | 44158591 | 44158462 | ID=cds.MS.gene25599.t1;Parent=MS.gene25599.t1 |
| chr8.2 | exon | 44159300 | 44159478 | 44159300 | ID=MS.gene25599.t1.exon20;Parent=MS.gene25599.t1 |
| chr8.2 | CDS | 44159300 | 44159478 | 44159300 | ID=cds.MS.gene25599.t1;Parent=MS.gene25599.t1 |
| Gene Sequence |
| Protein sequence |
