Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29822.t1 | XP_013460895.1 | 96.8 | 220 | 7 | 0 | 1 | 220 | 1 | 220 | 3.30E-117 | 431 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29822.t1 | A0A072UYT0 | 96.8 | 220 | 7 | 0 | 1 | 220 | 1 | 220 | 2.4e-117 | 431.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049260 | MS.gene29822 | 0.807429 | 5.08E-50 | -1.69E-46 |
| MS.gene049758 | MS.gene29822 | 0.823214 | 1.56E-53 | -1.69E-46 |
| MS.gene050020 | MS.gene29822 | 0.809045 | 2.30E-50 | -1.69E-46 |
| MS.gene050128 | MS.gene29822 | 0.826632 | 2.44E-54 | -1.69E-46 |
| MS.gene050584 | MS.gene29822 | 0.832847 | 7.48E-56 | -1.69E-46 |
| MS.gene050586 | MS.gene29822 | 0.834171 | 3.50E-56 | -1.69E-46 |
| MS.gene050587 | MS.gene29822 | 0.800002 | 1.77E-48 | -1.69E-46 |
| MS.gene051626 | MS.gene29822 | 0.838143 | 3.43E-57 | -1.69E-46 |
| MS.gene051663 | MS.gene29822 | 0.804243 | 2.37E-49 | -1.69E-46 |
| MS.gene051789 | MS.gene29822 | 0.891391 | 4.15E-74 | -1.69E-46 |
| MS.gene051860 | MS.gene29822 | 0.803378 | 3.59E-49 | -1.69E-46 |
| MS.gene052142 | MS.gene29822 | 0.816217 | 6.20E-52 | -1.69E-46 |
| MS.gene052178 | MS.gene29822 | 0.861081 | 1.32E-63 | -1.69E-46 |
| MS.gene052967 | MS.gene29822 | 0.832026 | 1.20E-55 | -1.69E-46 |
| MS.gene053074 | MS.gene29822 | 0.871211 | 8.12E-67 | -1.69E-46 |
| MS.gene053204 | MS.gene29822 | 0.815121 | 1.09E-51 | -1.69E-46 |
| MS.gene053836 | MS.gene29822 | 0.820094 | 8.22E-53 | -1.69E-46 |
| MS.gene054011 | MS.gene29822 | 0.828087 | 1.09E-54 | -1.69E-46 |
| MS.gene054012 | MS.gene29822 | 0.820417 | 6.94E-53 | -1.69E-46 |
| MS.gene054013 | MS.gene29822 | 0.816441 | 5.52E-52 | -1.69E-46 |
| MS.gene054184 | MS.gene29822 | 0.831541 | 1.57E-55 | -1.69E-46 |
| MS.gene054185 | MS.gene29822 | 0.837666 | 4.54E-57 | -1.69E-46 |
| MS.gene054214 | MS.gene29822 | 0.864271 | 1.37E-64 | -1.69E-46 |
| MS.gene05456 | MS.gene29822 | 0.846321 | 2.34E-59 | -1.69E-46 |
| MS.gene054596 | MS.gene29822 | 0.825321 | 5.00E-54 | -1.69E-46 |
| MS.gene055133 | MS.gene29822 | 0.810027 | 1.41E-50 | -1.69E-46 |
| MS.gene055207 | MS.gene29822 | 0.833937 | 4.00E-56 | -1.69E-46 |
| MS.gene055422 | MS.gene29822 | 0.812154 | 4.89E-51 | -1.69E-46 |
| MS.gene055428 | MS.gene29822 | 0.808173 | 3.53E-50 | -1.69E-46 |
| MS.gene055604 | MS.gene29822 | 0.808283 | 3.34E-50 | -1.69E-46 |
| MS.gene056069 | MS.gene29822 | -0.816138 | 6.46E-52 | -1.69E-46 |
| MS.gene057754 | MS.gene29822 | 0.810919 | 9.07E-51 | -1.69E-46 |
| MS.gene058168 | MS.gene29822 | 0.827335 | 1.66E-54 | -1.69E-46 |
| MS.gene059006 | MS.gene29822 | 0.875319 | 3.38E-68 | -1.69E-46 |
| MS.gene059124 | MS.gene29822 | 0.814004 | 1.92E-51 | -1.69E-46 |
| MS.gene059228 | MS.gene29822 | 0.846282 | 2.40E-59 | -1.69E-46 |
| MS.gene059518 | MS.gene29822 | 0.804876 | 1.75E-49 | -1.69E-46 |
| MS.gene06037 | MS.gene29822 | 0.816972 | 4.20E-52 | -1.69E-46 |
| MS.gene060476 | MS.gene29822 | 0.829115 | 6.17E-55 | -1.69E-46 |
| MS.gene061049 | MS.gene29822 | 0.811129 | 8.17E-51 | -1.69E-46 |
| MS.gene061112 | MS.gene29822 | 0.815967 | 7.05E-52 | -1.69E-46 |
| MS.gene061265 | MS.gene29822 | 0.813495 | 2.49E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29822.t1 | MTR_3g074450 | 96.818 | 220 | 7 | 0 | 1 | 220 | 1 | 220 | 1.07e-159 | 440 |
| MS.gene29822.t1 | MTR_5g063720 | 53.540 | 226 | 82 | 10 | 1 | 217 | 1 | 212 | 3.83e-58 | 183 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29822.t1 | AT1G78170 | 34.804 | 204 | 111 | 8 | 18 | 211 | 21 | 212 | 2.00e-19 | 83.6 |
| MS.gene29822.t1 | AT1G22250 | 40.870 | 115 | 55 | 4 | 101 | 208 | 80 | 188 | 4.83e-16 | 73.9 |
| MS.gene29822.t1 | AT4G08910 | 40.000 | 120 | 45 | 3 | 102 | 203 | 86 | 196 | 1.16e-15 | 73.2 |
Find 43 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATGTTTCCTTCAAATAAT+TGG | 0.175659 | 3.4:+72971512 | MS.gene29822:CDS |
| TTGAAGAATTCTTCAGTAAA+TGG | 0.216211 | 3.4:-72971430 | None:intergenic |
| GTGTCAATACCTTGGTATTC+AGG | 0.222403 | 3.4:+72971534 | MS.gene29822:CDS |
| TCTTCTTTAACTTCAGTTCT+TGG | 0.222864 | 3.4:-72971373 | None:intergenic |
| CATGGACAGTGCTTTGAATA+AGG | 0.336246 | 3.4:-72971347 | None:intergenic |
| CAGCTTCTTCTGCTCTAGTT+AGG | 0.340324 | 3.4:+72972075 | MS.gene29822:CDS |
| AGCAAGTTTAAGAAAGATTC+GGG | 0.373167 | 3.4:+72971869 | MS.gene29822:CDS |
| AAGCAAGTTTAAGAAAGATT+CGG | 0.375998 | 3.4:+72971868 | MS.gene29822:CDS |
| CATCTCTTTGTAATCTCCTT+CGG | 0.379098 | 3.4:-72971927 | None:intergenic |
| ATCATGTTCTGTCAAACTCT+TGG | 0.401354 | 3.4:-72971828 | None:intergenic |
| AAAGATTCGGGTGGTTTAAA+CGG | 0.409779 | 3.4:+72971881 | MS.gene29822:CDS |
| GTTTCTATCGTTGTCCGATC+GGG | 0.412127 | 3.4:-72971680 | None:intergenic |
| ATTTACTGAAGAATTCTTCA+AGG | 0.415230 | 3.4:+72971432 | MS.gene29822:CDS |
| ATCAAACAAATTTAGAGAAA+AGG | 0.427149 | 3.4:+72971455 | MS.gene29822:CDS |
| TGAAGGAAACATGGTCTCTA+GGG | 0.443563 | 3.4:-72971503 | None:intergenic |
| GGTTTCTATCGTTGTCCGAT+CGG | 0.443816 | 3.4:-72971681 | None:intergenic |
| TATTGACACCAATTATTTGA+AGG | 0.449231 | 3.4:-72971520 | None:intergenic |
| TTGTCGCAGGCTTCGTTTCG+AGG | 0.449883 | 3.4:-72971702 | None:intergenic |
| TCATGTTCTGTCAAACTCTT+GGG | 0.450709 | 3.4:-72971827 | None:intergenic |
| TTGAAGGAAACATGGTCTCT+AGG | 0.459950 | 3.4:-72971504 | None:intergenic |
| AGTGATCAGAAGAAGAATTC+TGG | 0.461088 | 3.4:+72971797 | MS.gene29822:CDS |
| AACTTCAGTTCTTGGAGTCA+TGG | 0.494815 | 3.4:-72971365 | None:intergenic |
| ATCAAAGAAAAGAAAGTTTG+AGG | 0.498556 | 3.4:+72971405 | MS.gene29822:CDS |
| GAGTGCATGAATTTGCAGTT+AGG | 0.515352 | 3.4:-72972013 | None:intergenic |
| AATAATTGGTGTCAATACCT+TGG | 0.524933 | 3.4:+72971526 | MS.gene29822:CDS |
| GTTTAAACGGTTCTCAGTCG+TGG | 0.546535 | 3.4:+72971894 | MS.gene29822:CDS |
| TAGGGGAGGCTTTGTATCAA+AGG | 0.546793 | 3.4:-72971485 | None:intergenic |
| AATTTGCAGTTAGGACAAGC+AGG | 0.557243 | 3.4:-72972004 | None:intergenic |
| CCAATTATTTGAAGGAAACA+TGG | 0.558834 | 3.4:-72971512 | None:intergenic |
| CGAAGGAGATTACAAAGAGA+TGG | 0.571998 | 3.4:+72971928 | MS.gene29822:CDS |
| CACATGTCAAGTTTAGCTCA+AGG | 0.577317 | 3.4:-72971751 | None:intergenic |
| TGCTTTGAATAAGGATACCA+TGG | 0.582273 | 3.4:-72971338 | None:intergenic |
| AAGCTCATGTGATCAGAAGA+AGG | 0.583375 | 3.4:-72971725 | None:intergenic |
| GGGAGGCTTTGTATCAAAGG+TGG | 0.591243 | 3.4:-72971482 | None:intergenic |
| GATCAGAAGAAGGTTGTCGC+AGG | 0.608082 | 3.4:-72971715 | None:intergenic |
| TCGTGGTTGTCGACAACCGA+AGG | 0.619983 | 3.4:+72971911 | MS.gene29822:CDS |
| TGTGTAACACAAGACCCGAT+CGG | 0.652579 | 3.4:+72971666 | MS.gene29822:CDS |
| ACTTGACATGTGAATCACAA+AGG | 0.656443 | 3.4:+72971762 | MS.gene29822:CDS |
| GGAAACATGGTCTCTAGGGG+AGG | 0.657415 | 3.4:-72971499 | None:intergenic |
| TTGCATGCAGTGTCACATGT+TGG | 0.659365 | 3.4:+72971961 | MS.gene29822:CDS |
| GAAGGAAACATGGTCTCTAG+GGG | 0.665977 | 3.4:-72971502 | None:intergenic |
| AAGTTTAAGAAAGATTCGGG+TGG | 0.705138 | 3.4:+72971872 | MS.gene29822:CDS |
| CAACATATACCTGAATACCA+AGG | 0.712025 | 3.4:-72971543 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCAAACAAATTTAGAGAAA+AGG | + | chr3.4:72971455-72971474 | MS.gene29822:CDS | 20.0% |
| !! | ATCATAAGATATGATATCAT+TGG | - | chr3.4:72971604-72971623 | None:intergenic | 20.0% |
| ! | AAGCAAGTTTAAGAAAGATT+CGG | + | chr3.4:72971868-72971887 | MS.gene29822:CDS | 25.0% |
| ! | ATCAAAGAAAAGAAAGTTTG+AGG | + | chr3.4:72971405-72971424 | MS.gene29822:CDS | 25.0% |
| ! | ATTTACTGAAGAATTCTTCA+AGG | + | chr3.4:72971432-72971451 | MS.gene29822:CDS | 25.0% |
| ! | TATTGACACCAATTATTTGA+AGG | - | chr3.4:72971523-72971542 | None:intergenic | 25.0% |
| ! | TTGAAGAATTCTTCAGTAAA+TGG | - | chr3.4:72971433-72971452 | None:intergenic | 25.0% |
| !!! | AATTTTTTTTATGTGCAGTC+AGG | + | chr3.4:72971632-72971651 | MS.gene29822:intron | 25.0% |
| !!! | ATTTTTTTTATGTGCAGTCA+GGG | + | chr3.4:72971633-72971652 | MS.gene29822:intron | 25.0% |
| AGCAAGTTTAAGAAAGATTC+GGG | + | chr3.4:72971869-72971888 | MS.gene29822:CDS | 30.0% | |
| CCAATTATTTGAAGGAAACA+TGG | - | chr3.4:72971515-72971534 | None:intergenic | 30.0% | |
| CCATGTTTCCTTCAAATAAT+TGG | + | chr3.4:72971512-72971531 | MS.gene29822:CDS | 30.0% | |
| ! | TCTTCTTTAACTTCAGTTCT+TGG | - | chr3.4:72971376-72971395 | None:intergenic | 30.0% |
| ! | TGCTCTTCAAGAATTTTGAA+GGG | - | chr3.4:72972051-72972070 | None:intergenic | 30.0% |
| !! | AATAATTGGTGTCAATACCT+TGG | + | chr3.4:72971526-72971545 | MS.gene29822:CDS | 30.0% |
| AAAGATTCGGGTGGTTTAAA+CGG | + | chr3.4:72971881-72971900 | MS.gene29822:CDS | 35.0% | |
| AAGTTTAAGAAAGATTCGGG+TGG | + | chr3.4:72971872-72971891 | MS.gene29822:CDS | 35.0% | |
| ACTTGACATGTGAATCACAA+AGG | + | chr3.4:72971762-72971781 | MS.gene29822:CDS | 35.0% | |
| AGTGATCAGAAGAAGAATTC+TGG | + | chr3.4:72971797-72971816 | MS.gene29822:CDS | 35.0% | |
| ATCATGTTCTGTCAAACTCT+TGG | - | chr3.4:72971831-72971850 | None:intergenic | 35.0% | |
| CAACATATACCTGAATACCA+AGG | - | chr3.4:72971546-72971565 | None:intergenic | 35.0% | |
| CATCTCTTTGTAATCTCCTT+CGG | - | chr3.4:72971930-72971949 | None:intergenic | 35.0% | |
| TCATGTTCTGTCAAACTCTT+GGG | - | chr3.4:72971830-72971849 | None:intergenic | 35.0% | |
| ! | CTGCTCTTCAAGAATTTTGA+AGG | - | chr3.4:72972052-72972071 | None:intergenic | 35.0% |
| !! | TCAAGAATTTTGAAGGGTTC+TGG | - | chr3.4:72972045-72972064 | None:intergenic | 35.0% |
| AACTTCAGTTCTTGGAGTCA+TGG | - | chr3.4:72971368-72971387 | None:intergenic | 40.0% | |
| AAGCTCATGTGATCAGAAGA+AGG | - | chr3.4:72971728-72971747 | None:intergenic | 40.0% | |
| AATTTGCAGTTAGGACAAGC+AGG | - | chr3.4:72972007-72972026 | None:intergenic | 40.0% | |
| CGAAGGAGATTACAAAGAGA+TGG | + | chr3.4:72971928-72971947 | MS.gene29822:CDS | 40.0% | |
| GAGTGCATGAATTTGCAGTT+AGG | - | chr3.4:72972016-72972035 | None:intergenic | 40.0% | |
| GTGTCAATACCTTGGTATTC+AGG | + | chr3.4:72971534-72971553 | MS.gene29822:CDS | 40.0% | |
| TGAAGGAAACATGGTCTCTA+GGG | - | chr3.4:72971506-72971525 | None:intergenic | 40.0% | |
| TTGAAGGAAACATGGTCTCT+AGG | - | chr3.4:72971507-72971526 | None:intergenic | 40.0% | |
| ! | CACATGTCAAGTTTAGCTCA+AGG | - | chr3.4:72971754-72971773 | None:intergenic | 40.0% |
| ! | CATGGACAGTGCTTTGAATA+AGG | - | chr3.4:72971350-72971369 | None:intergenic | 40.0% |
| !! | AATTTTGAAGGGTTCTGGTC+TGG | - | chr3.4:72972040-72972059 | None:intergenic | 40.0% |
| GAAGGAAACATGGTCTCTAG+GGG | - | chr3.4:72971505-72971524 | None:intergenic | 45.0% | |
| GGTTTCTATCGTTGTCCGAT+CGG | - | chr3.4:72971684-72971703 | None:intergenic | 45.0% | |
| GTTTAAACGGTTCTCAGTCG+TGG | + | chr3.4:72971894-72971913 | MS.gene29822:CDS | 45.0% | |
| GTTTCTATCGTTGTCCGATC+GGG | - | chr3.4:72971683-72971702 | None:intergenic | 45.0% | |
| TGTGTAACACAAGACCCGAT+CGG | + | chr3.4:72971666-72971685 | MS.gene29822:CDS | 45.0% | |
| TTGCATGCAGTGTCACATGT+TGG | + | chr3.4:72971961-72971980 | MS.gene29822:CDS | 45.0% | |
| ! | TAGGGGAGGCTTTGTATCAA+AGG | - | chr3.4:72971488-72971507 | None:intergenic | 45.0% |
| GATCAGAAGAAGGTTGTCGC+AGG | - | chr3.4:72971718-72971737 | None:intergenic | 50.0% | |
| ! | GGGAGGCTTTGTATCAAAGG+TGG | - | chr3.4:72971485-72971504 | None:intergenic | 50.0% |
| TCGTGGTTGTCGACAACCGA+AGG | + | chr3.4:72971911-72971930 | MS.gene29822:CDS | 55.0% | |
| TTGTCGCAGGCTTCGTTTCG+AGG | - | chr3.4:72971705-72971724 | None:intergenic | 55.0% | |
| ! | GGAAACATGGTCTCTAGGGG+AGG | - | chr3.4:72971502-72971521 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 72971340 | 72972096 | 72971340 | ID=MS.gene29822 |
| chr3.4 | mRNA | 72971340 | 72972096 | 72971340 | ID=MS.gene29822.t1;Parent=MS.gene29822 |
| chr3.4 | exon | 72971340 | 72971555 | 72971340 | ID=MS.gene29822.t1.exon1;Parent=MS.gene29822.t1 |
| chr3.4 | CDS | 72971340 | 72971555 | 72971340 | ID=cds.MS.gene29822.t1;Parent=MS.gene29822.t1 |
| chr3.4 | exon | 72971650 | 72972096 | 72971650 | ID=MS.gene29822.t1.exon2;Parent=MS.gene29822.t1 |
| chr3.4 | CDS | 72971650 | 72972096 | 72971650 | ID=cds.MS.gene29822.t1;Parent=MS.gene29822.t1 |
| Gene Sequence |
| Protein sequence |
