Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33065.t1 | XP_013465872.1 | 98.7 | 149 | 2 | 0 | 1 | 149 | 1 | 149 | 1.90E-76 | 295 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33065.t1 | A0A072VER7 | 98.7 | 149 | 2 | 0 | 1 | 149 | 1 | 149 | 1.4e-76 | 295.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene33065 | 0.812104 | 5.01E-51 | -1.69E-46 |
| MS.gene049151 | MS.gene33065 | 0.814758 | 1.31E-51 | -1.69E-46 |
| MS.gene049153 | MS.gene33065 | 0.811931 | 5.47E-51 | -1.69E-46 |
| MS.gene049230 | MS.gene33065 | 0.804481 | 2.12E-49 | -1.69E-46 |
| MS.gene049378 | MS.gene33065 | 0.839961 | 1.16E-57 | -1.69E-46 |
| MS.gene049758 | MS.gene33065 | 0.83163 | 1.50E-55 | -1.69E-46 |
| MS.gene050128 | MS.gene33065 | 0.826631 | 2.44E-54 | -1.69E-46 |
| MS.gene050623 | MS.gene33065 | -0.837793 | 4.21E-57 | -1.69E-46 |
| MS.gene050642 | MS.gene33065 | 0.837085 | 6.40E-57 | -1.69E-46 |
| MS.gene050762 | MS.gene33065 | 0.822379 | 2.44E-53 | -1.69E-46 |
| MS.gene050763 | MS.gene33065 | 0.822734 | 2.02E-53 | -1.69E-46 |
| MS.gene051535 | MS.gene33065 | 0.934497 | 3.69E-96 | -1.69E-46 |
| MS.gene051860 | MS.gene33065 | 0.812028 | 5.21E-51 | -1.69E-46 |
| MS.gene051983 | MS.gene33065 | 0.851486 | 8.60E-61 | -1.69E-46 |
| MS.gene052077 | MS.gene33065 | 0.845578 | 3.72E-59 | -1.69E-46 |
| MS.gene052426 | MS.gene33065 | 0.834837 | 2.38E-56 | -1.69E-46 |
| MS.gene053073 | MS.gene33065 | 0.802676 | 5.01E-49 | -1.69E-46 |
| MS.gene05316 | MS.gene33065 | 0.849507 | 3.09E-60 | -1.69E-46 |
| MS.gene053494 | MS.gene33065 | 0.852676 | 3.95E-61 | -1.69E-46 |
| MS.gene053559 | MS.gene33065 | 0.812989 | 3.21E-51 | -1.69E-46 |
| MS.gene053836 | MS.gene33065 | 0.818308 | 2.10E-52 | -1.69E-46 |
| MS.gene054379 | MS.gene33065 | 0.832743 | 7.94E-56 | -1.69E-46 |
| MS.gene054930 | MS.gene33065 | 0.825945 | 3.55E-54 | -1.69E-46 |
| MS.gene05510 | MS.gene33065 | 0.805148 | 1.54E-49 | -1.69E-46 |
| MS.gene055701 | MS.gene33065 | 0.844338 | 8.04E-59 | -1.69E-46 |
| MS.gene056979 | MS.gene33065 | 0.845587 | 3.70E-59 | -1.69E-46 |
| MS.gene057396 | MS.gene33065 | 0.822637 | 2.13E-53 | -1.69E-46 |
| MS.gene057750 | MS.gene33065 | 0.805574 | 1.25E-49 | -1.69E-46 |
| MS.gene058146 | MS.gene33065 | 0.809556 | 1.79E-50 | -1.69E-46 |
| MS.gene058435 | MS.gene33065 | 0.979052 | 3.96E-147 | -1.69E-46 |
| MS.gene058595 | MS.gene33065 | 0.802755 | 4.83E-49 | -1.69E-46 |
| MS.gene059285 | MS.gene33065 | 0.811315 | 7.45E-51 | -1.69E-46 |
| MS.gene05984 | MS.gene33065 | 0.801175 | 1.02E-48 | -1.69E-46 |
| MS.gene059899 | MS.gene33065 | 0.828733 | 7.63E-55 | -1.69E-46 |
| MS.gene060084 | MS.gene33065 | 0.816127 | 6.49E-52 | -1.69E-46 |
| MS.gene060495 | MS.gene33065 | 0.839181 | 1.85E-57 | -1.69E-46 |
| MS.gene060683 | MS.gene33065 | 0.817805 | 2.73E-52 | -1.69E-46 |
| MS.gene060685 | MS.gene33065 | 0.80852 | 2.97E-50 | -1.69E-46 |
| MS.gene060802 | MS.gene33065 | 0.800555 | 1.36E-48 | -1.69E-46 |
| MS.gene060940 | MS.gene33065 | 0.805565 | 1.26E-49 | -1.69E-46 |
| MS.gene060943 | MS.gene33065 | 0.821041 | 4.98E-53 | -1.69E-46 |
| MS.gene061002 | MS.gene33065 | 0.815493 | 8.99E-52 | -1.69E-46 |
| MS.gene061049 | MS.gene33065 | 0.805823 | 1.11E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33065.t1 | MTR_1g014930 | 98.658 | 149 | 2 | 0 | 1 | 149 | 1 | 149 | 8.26e-108 | 303 |
| MS.gene33065.t1 | MTR_3g105340 | 54.839 | 124 | 45 | 4 | 13 | 135 | 11 | 124 | 2.44e-37 | 124 |
| MS.gene33065.t1 | MTR_8g021190 | 46.400 | 125 | 54 | 5 | 20 | 136 | 34 | 153 | 4.21e-30 | 107 |
| MS.gene33065.t1 | MTR_4g125030 | 52.727 | 110 | 44 | 3 | 6 | 113 | 15 | 118 | 5.45e-30 | 105 |
| MS.gene33065.t1 | MTR_4g125010 | 48.649 | 111 | 48 | 3 | 6 | 113 | 17 | 121 | 2.14e-28 | 102 |
| MS.gene33065.t1 | MTR_3g109860 | 37.374 | 99 | 58 | 2 | 13 | 110 | 33 | 128 | 1.63e-17 | 74.3 |
| MS.gene33065.t1 | MTR_1g103550 | 45.070 | 71 | 37 | 1 | 19 | 89 | 5 | 73 | 2.66e-17 | 73.2 |
| MS.gene33065.t1 | MTR_5g006430 | 39.560 | 91 | 49 | 2 | 17 | 107 | 3 | 87 | 3.55e-17 | 72.8 |
| MS.gene33065.t1 | MTR_8g013990 | 31.724 | 145 | 82 | 5 | 1 | 136 | 47 | 183 | 6.58e-16 | 71.2 |
| MS.gene33065.t1 | MTR_2g101140 | 45.455 | 77 | 31 | 2 | 17 | 86 | 63 | 135 | 1.76e-15 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33065.t1 | AT1G72510 | 43.939 | 132 | 57 | 5 | 19 | 136 | 36 | 164 | 7.97e-30 | 106 |
| MS.gene33065.t1 | AT1G72510 | 43.939 | 132 | 57 | 5 | 19 | 136 | 36 | 164 | 7.97e-30 | 106 |
| MS.gene33065.t1 | AT5G20670 | 43.478 | 138 | 63 | 3 | 11 | 136 | 17 | 151 | 1.26e-28 | 103 |
| MS.gene33065.t1 | AT2G09970 | 37.931 | 145 | 71 | 6 | 6 | 136 | 26 | 165 | 4.19e-24 | 92.0 |
| MS.gene33065.t1 | AT1G54095 | 44.037 | 109 | 59 | 2 | 1 | 109 | 1 | 107 | 1.63e-20 | 82.4 |
| MS.gene33065.t1 | AT3G22540 | 37.500 | 120 | 59 | 5 | 13 | 131 | 2 | 106 | 6.24e-19 | 77.0 |
| MS.gene33065.t1 | AT4G14819 | 36.134 | 119 | 59 | 4 | 13 | 131 | 2 | 103 | 1.21e-17 | 73.6 |
| MS.gene33065.t1 | AT2G25780 | 35.537 | 121 | 70 | 4 | 20 | 138 | 39 | 153 | 3.12e-14 | 66.2 |
| MS.gene33065.t1 | AT1G79770 | 70.000 | 40 | 12 | 0 | 17 | 56 | 52 | 91 | 1.15e-13 | 65.1 |
| MS.gene33065.t1 | AT5G25840 | 37.008 | 127 | 60 | 6 | 20 | 136 | 46 | 162 | 3.06e-13 | 63.9 |
Find 30 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGAACTGCTGGCGAAATT+TGG | 0.269881 | 1.1:-6738232 | None:intergenic |
| CCGCAAAATGCAGCTGTTGC+GGG | 0.402544 | 1.1:+6738049 | MS.gene33065:CDS |
| CACGCAGAATGTTCCTTACT+CGG | 0.438740 | 1.1:-6738339 | None:intergenic |
| TTAGGACTTTGGATTCTCCT+AGG | 0.457929 | 1.1:+6738310 | MS.gene33065:CDS |
| TCCTAGGAGCAAGCCGAGTA+AGG | 0.469773 | 1.1:+6738326 | MS.gene33065:CDS |
| GGGAGAGATTCGGTGGAAGA+TGG | 0.469879 | 1.1:+6738112 | MS.gene33065:CDS |
| GTATGTGTCTCTGTCGTCCT+TGG | 0.478341 | 1.1:-6738414 | None:intergenic |
| ACCGCAAAATGCAGCTGTTG+CGG | 0.482069 | 1.1:+6738048 | MS.gene33065:CDS |
| CTGAGGCAGTGAAGGAAGAA+AGG | 0.486207 | 1.1:+6738151 | MS.gene33065:CDS |
| CATTCTGCGTGCAGACCTCT+TGG | 0.504691 | 1.1:+6738351 | MS.gene33065:CDS |
| AAGCAGAAGATCTGAACTGC+TGG | 0.512505 | 1.1:-6738242 | None:intergenic |
| GAAGGAAGAAAGGGCGAGGT+CGG | 0.514158 | 1.1:+6738161 | MS.gene33065:CDS |
| ATGCAGCTGTTGCGGGTTGG+TGG | 0.517846 | 1.1:+6738056 | MS.gene33065:CDS |
| AGAAGCTGAAGCGACAGAGA+TGG | 0.523368 | 1.1:+6738014 | MS.gene33065:CDS |
| TCCTTACTCGGCTTGCTCCT+AGG | 0.526193 | 1.1:-6738327 | None:intergenic |
| TGTGTCTCTGTCGTCCTTGG+CGG | 0.528871 | 1.1:-6738411 | None:intergenic |
| GCGCGCGTGAGGGAGAGATT+CGG | 0.542153 | 1.1:+6738102 | MS.gene33065:CDS |
| ACGCGTACATTGCGCGCGTG+AGG | 0.548939 | 1.1:+6738091 | MS.gene33065:CDS |
| CGCGTACATTGCGCGCGTGA+GGG | 0.561079 | 1.1:+6738092 | MS.gene33065:CDS |
| TCATAGTTGCTTCTCAACAA+TGG | 0.575206 | 1.1:+6738380 | MS.gene33065:CDS |
| TGAGGCAGTGAAGGAAGAAA+GGG | 0.590842 | 1.1:+6738152 | MS.gene33065:CDS |
| CAGTGAAGGAAGAAAGGGCG+AGG | 0.593016 | 1.1:+6738157 | MS.gene33065:CDS |
| TTTATGTGCTGAGGCAGTGA+AGG | 0.603888 | 1.1:+6738143 | MS.gene33065:CDS |
| CGCGTGAGGGAGAGATTCGG+TGG | 0.607365 | 1.1:+6738105 | MS.gene33065:CDS |
| AAAATGCAGCTGTTGCGGGT+TGG | 0.633011 | 1.1:+6738053 | MS.gene33065:CDS |
| CAATGGAAACAACTCCGCCA+AGG | 0.641855 | 1.1:+6738397 | MS.gene33065:CDS |
| TTCGGTGGAAGATGGATATG+CGG | 0.649354 | 1.1:+6738120 | MS.gene33065:CDS |
| CAACTATGAGATCTACCAAG+AGG | 0.689504 | 1.1:-6738366 | None:intergenic |
| CAATTGATGAAGCTTTAATG+CGG | 0.691551 | 1.1:+6738205 | MS.gene33065:CDS |
| GATATGCGGTTTATGTGCTG+AGG | 0.715926 | 1.1:+6738134 | MS.gene33065:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CAATTGATGAAGCTTTAATG+CGG | + | chr1.1:6738205-6738224 | MS.gene33065:CDS | 30.0% |
| ! | CTGTGAAACAGATTCTTTTT+AGG | + | chr1.1:6738292-6738311 | MS.gene33065:CDS | 30.0% |
| !!! | ACAGATTCTTTTTAGGACTT+TGG | + | chr1.1:6738299-6738318 | MS.gene33065:CDS | 30.0% |
| TCATAGTTGCTTCTCAACAA+TGG | + | chr1.1:6738380-6738399 | MS.gene33065:CDS | 35.0% | |
| CAACTATGAGATCTACCAAG+AGG | - | chr1.1:6738369-6738388 | None:intergenic | 40.0% | |
| TTAGGACTTTGGATTCTCCT+AGG | + | chr1.1:6738310-6738329 | MS.gene33065:CDS | 40.0% | |
| AAGCAGAAGATCTGAACTGC+TGG | - | chr1.1:6738245-6738264 | None:intergenic | 45.0% | |
| CACGCAGAATGTTCCTTACT+CGG | - | chr1.1:6738342-6738361 | None:intergenic | 45.0% | |
| GATATGCGGTTTATGTGCTG+AGG | + | chr1.1:6738134-6738153 | MS.gene33065:CDS | 45.0% | |
| TCTGAACTGCTGGCGAAATT+TGG | - | chr1.1:6738235-6738254 | None:intergenic | 45.0% | |
| TGAGGCAGTGAAGGAAGAAA+GGG | + | chr1.1:6738152-6738171 | MS.gene33065:CDS | 45.0% | |
| TTCGGTGGAAGATGGATATG+CGG | + | chr1.1:6738120-6738139 | MS.gene33065:CDS | 45.0% | |
| TTTATGTGCTGAGGCAGTGA+AGG | + | chr1.1:6738143-6738162 | MS.gene33065:CDS | 45.0% | |
| AAAATGCAGCTGTTGCGGGT+TGG | + | chr1.1:6738053-6738072 | MS.gene33065:CDS | 50.0% | |
| ACCGCAAAATGCAGCTGTTG+CGG | + | chr1.1:6738048-6738067 | MS.gene33065:CDS | 50.0% | |
| AGAAGCTGAAGCGACAGAGA+TGG | + | chr1.1:6738014-6738033 | MS.gene33065:CDS | 50.0% | |
| CAATGGAAACAACTCCGCCA+AGG | + | chr1.1:6738397-6738416 | MS.gene33065:CDS | 50.0% | |
| CTGAGGCAGTGAAGGAAGAA+AGG | + | chr1.1:6738151-6738170 | MS.gene33065:CDS | 50.0% | |
| GTATGTGTCTCTGTCGTCCT+TGG | - | chr1.1:6738417-6738436 | None:intergenic | 50.0% | |
| CAGTGAAGGAAGAAAGGGCG+AGG | + | chr1.1:6738157-6738176 | MS.gene33065:CDS | 55.0% | |
| CATTCTGCGTGCAGACCTCT+TGG | + | chr1.1:6738351-6738370 | MS.gene33065:CDS | 55.0% | |
| CCGCAAAATGCAGCTGTTGC+GGG | + | chr1.1:6738049-6738068 | MS.gene33065:CDS | 55.0% | |
| GAAGGAAGAAAGGGCGAGGT+CGG | + | chr1.1:6738161-6738180 | MS.gene33065:CDS | 55.0% | |
| TCCTAGGAGCAAGCCGAGTA+AGG | + | chr1.1:6738326-6738345 | MS.gene33065:CDS | 55.0% | |
| TGTGTCTCTGTCGTCCTTGG+CGG | - | chr1.1:6738414-6738433 | None:intergenic | 55.0% | |
| ! | CCCGCAACAGCTGCATTTTG+CGG | - | chr1.1:6738052-6738071 | None:intergenic | 55.0% |
| ! | TCCTTACTCGGCTTGCTCCT+AGG | - | chr1.1:6738330-6738349 | None:intergenic | 55.0% |
| !! | GGGAGAGATTCGGTGGAAGA+TGG | + | chr1.1:6738112-6738131 | MS.gene33065:CDS | 55.0% |
| !! | ATGCAGCTGTTGCGGGTTGG+TGG | + | chr1.1:6738056-6738075 | MS.gene33065:CDS | 60.0% |
| ACGCGTACATTGCGCGCGTG+AGG | + | chr1.1:6738091-6738110 | MS.gene33065:CDS | 65.0% | |
| CGCGTACATTGCGCGCGTGA+GGG | + | chr1.1:6738092-6738111 | MS.gene33065:CDS | 65.0% | |
| CGCGTGAGGGAGAGATTCGG+TGG | + | chr1.1:6738105-6738124 | MS.gene33065:CDS | 65.0% | |
| GCGCGCGTGAGGGAGAGATT+CGG | + | chr1.1:6738102-6738121 | MS.gene33065:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 6737994 | 6738443 | 6737994 | ID=MS.gene33065 |
| chr1.1 | mRNA | 6737994 | 6738443 | 6737994 | ID=MS.gene33065.t1;Parent=MS.gene33065 |
| chr1.1 | exon | 6737994 | 6738443 | 6737994 | ID=MS.gene33065.t1.exon1;Parent=MS.gene33065.t1 |
| chr1.1 | CDS | 6737994 | 6738443 | 6737994 | ID=cds.MS.gene33065.t1;Parent=MS.gene33065.t1 |
| Gene Sequence |
| Protein sequence |
