AlfalfaGEDB | MS.gene34515

M. sativa cultivar XinJiangDaYe / MS.gene34515

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34515.t1	XP_013444013.1	86.3	161	22	0	1	161	1	161	1.90E-50	208.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34515.t1	Q67ZB3	83.0	53	9	0	1	53	1	53	2.0e-16	87.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34515.t1	A0A072TWW9	86.3	161	22	0	1	161	1	161	1.4e-50	208.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene34515	0.832477	9.24E-56	-1.69E-46
MS.gene049193	MS.gene34515	0.820697	5.98E-53	-1.69E-46
MS.gene049368	MS.gene34515	0.816061	6.71E-52	-1.69E-46
MS.gene049499	MS.gene34515	0.801078	1.07E-48	-1.69E-46
MS.gene049807	MS.gene34515	0.800835	1.20E-48	-1.69E-46
MS.gene049983	MS.gene34515	0.803087	4.12E-49	-1.69E-46
MS.gene050084	MS.gene34515	0.834711	2.56E-56	-1.69E-46
MS.gene050085	MS.gene34515	0.845251	4.56E-59	-1.69E-46
MS.gene050213	MS.gene34515	0.823186	1.59E-53	-1.69E-46
MS.gene050359	MS.gene34515	0.841078	5.91E-58	-1.69E-46
MS.gene050384	MS.gene34515	0.806583	7.67E-50	-1.69E-46
MS.gene050386	MS.gene34515	0.808419	3.13E-50	-1.69E-46
MS.gene050551	MS.gene34515	0.82527	5.14E-54	-1.69E-46
MS.gene050646	MS.gene34515	0.847495	1.12E-59	-1.69E-46
MS.gene050654	MS.gene34515	0.812266	4.62E-51	-1.69E-46
MS.gene050689	MS.gene34515	0.804292	2.32E-49	-1.69E-46
MS.gene050945	MS.gene34515	0.808725	2.69E-50	-1.69E-46
MS.gene051234	MS.gene34515	0.827139	1.85E-54	-1.69E-46
MS.gene051343	MS.gene34515	0.832676	8.25E-56	-1.69E-46
MS.gene051381	MS.gene34515	0.815324	9.80E-52	-1.69E-46
MS.gene052036	MS.gene34515	0.802388	5.74E-49	-1.69E-46
MS.gene052072	MS.gene34515	0.81779	2.75E-52	-1.69E-46
MS.gene052242	MS.gene34515	-0.835129	2.01E-56	-1.69E-46
MS.gene052307	MS.gene34515	0.830488	2.86E-55	-1.69E-46
MS.gene052342	MS.gene34515	0.80868	2.75E-50	-1.69E-46
MS.gene052514	MS.gene34515	0.844574	6.95E-59	-1.69E-46
MS.gene052516	MS.gene34515	0.808288	3.33E-50	-1.69E-46
MS.gene052609	MS.gene34515	0.867684	1.14E-65	-1.69E-46
MS.gene052776	MS.gene34515	0.809834	1.56E-50	-1.69E-46
MS.gene053143	MS.gene34515	0.860451	2.05E-63	-1.69E-46
MS.gene05315	MS.gene34515	0.815386	9.49E-52	-1.69E-46
MS.gene053541	MS.gene34515	0.800502	1.40E-48	-1.69E-46
MS.gene053542	MS.gene34515	0.812581	3.94E-51	-1.69E-46
MS.gene053620	MS.gene34515	0.803922	2.77E-49	-1.69E-46
MS.gene054178	MS.gene34515	0.804446	2.15E-49	-1.69E-46
MS.gene055469	MS.gene34515	0.807726	4.39E-50	-1.69E-46
MS.gene055513	MS.gene34515	0.819108	1.38E-52	-1.69E-46
MS.gene056045	MS.gene34515	0.806958	6.39E-50	-1.69E-46
MS.gene056097	MS.gene34515	0.811654	6.29E-51	-1.69E-46
MS.gene056181	MS.gene34515	0.819367	1.21E-52	-1.69E-46
MS.gene056308	MS.gene34515	0.846078	2.72E-59	-1.69E-46
MS.gene056507	MS.gene34515	0.818929	1.52E-52	-1.69E-46
MS.gene056519	MS.gene34515	0.828677	7.87E-55	-1.69E-46
MS.gene056578	MS.gene34515	0.818908	1.53E-52	-1.69E-46
MS.gene056831	MS.gene34515	0.801949	7.07E-49	-1.69E-46
MS.gene057919	MS.gene34515	0.814336	1.62E-51	-1.69E-46
MS.gene058292	MS.gene34515	0.8014	9.17E-49	-1.69E-46
MS.gene058330	MS.gene34515	0.827585	1.44E-54	-1.69E-46
MS.gene059007	MS.gene34515	0.824054	9.94E-54	-1.69E-46
MS.gene059170	MS.gene34515	0.825301	5.05E-54	-1.69E-46
MS.gene059778	MS.gene34515	0.815735	7.94E-52	-1.69E-46
MS.gene060933	MS.gene34515	0.841693	4.07E-58	-1.69E-46
MS.gene060935	MS.gene34515	0.809763	1.61E-50	-1.69E-46
MS.gene060937	MS.gene34515	0.828004	1.14E-54	-1.69E-46
MS.gene061006	MS.gene34515	0.846865	1.66E-59	-1.69E-46
MS.gene061100	MS.gene34515	0.874959	4.49E-68	-1.69E-46
MS.gene061160	MS.gene34515	0.804274	2.34E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34515.t1	MTR_8g010170	96.273	161	6	0	1	161	1	161	1.74e-105	312
MS.gene34515.t1	MTR_8g010160	72.050	161	45	0	1	161	1	161	3.88e-77	239
MS.gene34515.t1	MTR_4g095080	38.776	98	59	1	16	112	14	111	6.47e-15	71.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34515.t1	AT5G48385	64.828	145	39	2	1	144	1	134	4.99e-46	158

Find 27 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GACTTGGCTACTGTCTTTAA+TGG	0.246449	8.1:-85747069	MS.gene34515:CDS
CTGTTACTCTTAACTTGAAA+TGG	0.318067	8.1:-85747335	MS.gene34515:CDS
TTCATTAAAACGCCTCTTCA+AGG	0.368337	8.1:+85747275	None:intergenic
TCGAGAAATGATAGAGAAAC+AGG	0.388435	8.1:-85747208	MS.gene34515:CDS
ACAGGTTTCTCCTTGACATC+TGG	0.390907	8.1:+85747033	None:intergenic
ACTTGGCTACTGTCTTTAAT+GGG	0.419239	8.1:-85747068	MS.gene34515:CDS
CTGTGGATGCTGTGTCCACT+AGG	0.452001	8.1:-85747014	MS.gene34515:CDS
CTTCTTTACTATGGAGGACA+AGG	0.477549	8.1:-85747442	None:intergenic
GAAGGTTTCATCAAAGGACT+TGG	0.515565	8.1:-85747085	MS.gene34515:CDS
CCTTTGAAGTCTCTGCAGCA+AGG	0.521025	8.1:+85747158	None:intergenic
TATTCACCATTGTAGATGCT+CGG	0.553561	8.1:-85747119	MS.gene34515:CDS
ATGTCCGCACTTACATTCGC+AGG	0.553714	8.1:+85746958	None:intergenic
GCTGTGTCCACTAGGGCTGA+AGG	0.565201	8.1:-85747006	MS.gene34515:CDS
GATGTTGCCTTCAGCCCTAG+TGG	0.578689	8.1:+85746999	None:intergenic
CCTTGCTGCAGAGACTTCAA+AGG	0.579253	8.1:-85747158	MS.gene34515:CDS
ATACAGGAAGGTTTCATCAA+AGG	0.582776	8.1:-85747091	MS.gene34515:CDS
TGTGGATGCTGTGTCCACTA+GGG	0.588731	8.1:-85747013	MS.gene34515:CDS
TCAAGCGGCACCAGATGTCA+AGG	0.595899	8.1:-85747043	MS.gene34515:CDS
GGCTGGAGAAATCCTTGAAG+AGG	0.602202	8.1:-85747287	MS.gene34515:CDS
TCAAGTTAAGAGTAACAGCT+CGG	0.607083	8.1:+85747340	None:intergenic
CTAGTGGACACAGCATCCAC+AGG	0.607123	8.1:+85747015	None:intergenic
AAAGATACAACAGCTGCAGA+AGG	0.613139	8.1:-85747385	MS.gene34515:CDS
AATGATAGAGAAACAGGAAG+CGG	0.629888	8.1:-85747202	MS.gene34515:CDS
CTTGAAATGGAAAGAACTCG+AGG	0.646861	8.1:-85747322	MS.gene34515:CDS
TGTCTTTAATGGGAGTCAAG+CGG	0.649347	8.1:-85747058	MS.gene34515:CDS
AAGAGTTTGAAAACAAGACG+AGG	0.680352	8.1:-85747236	MS.gene34515:CDS
AGATGTCAAGGAGAAACCTG+TGG	0.686737	8.1:-85747031	MS.gene34515:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CTGTTACTCTTAACTTGAAA+TGG	-	chr8.1:85747069-85747088	MS.gene34515:CDS	30.0%
!	TGTTTTCAAACTCTTTCTCT+TGG	+	chr8.1:85747163-85747182	None:intergenic	30.0%
	AAGAGTTTGAAAACAAGACG+AGG	-	chr8.1:85747168-85747187	MS.gene34515:CDS	35.0%
	AATGATAGAGAAACAGGAAG+CGG	-	chr8.1:85747202-85747221	MS.gene34515:CDS	35.0%
	ACTTGGCTACTGTCTTTAAT+GGG	-	chr8.1:85747336-85747355	MS.gene34515:CDS	35.0%
	ATACAGGAAGGTTTCATCAA+AGG	-	chr8.1:85747313-85747332	MS.gene34515:CDS	35.0%
	TAGATGCTCGGAAAAAATAC+AGG	-	chr8.1:85747297-85747316	MS.gene34515:CDS	35.0%
	TATTCACCATTGTAGATGCT+CGG	-	chr8.1:85747285-85747304	MS.gene34515:CDS	35.0%
	TCAAGTTAAGAGTAACAGCT+CGG	+	chr8.1:85747067-85747086	None:intergenic	35.0%
	TCGAGAAATGATAGAGAAAC+AGG	-	chr8.1:85747196-85747215	MS.gene34515:CDS	35.0%
	TTCATTAAAACGCCTCTTCA+AGG	+	chr8.1:85747132-85747151	None:intergenic	35.0%
!	AACTCGAGGAACATTTTCAA+GGG	-	chr8.1:85747096-85747115	MS.gene34515:CDS	35.0%
!!!	TTTTTTCCGAGCATCTACAA+TGG	+	chr8.1:85747294-85747313	None:intergenic	35.0%
	AAAGATACAACAGCTGCAGA+AGG	-	chr8.1:85747019-85747038	MS.gene34515:CDS	40.0%
	CTTGAAATGGAAAGAACTCG+AGG	-	chr8.1:85747082-85747101	MS.gene34515:CDS	40.0%
	GAAGGTTTCATCAAAGGACT+TGG	-	chr8.1:85747319-85747338	MS.gene34515:CDS	40.0%
	GACTTGGCTACTGTCTTTAA+TGG	-	chr8.1:85747335-85747354	MS.gene34515:CDS	40.0%
	TGCTCGGAAAAAATACAGGA+AGG	-	chr8.1:85747301-85747320	MS.gene34515:CDS	40.0%
	TGTCTTTAATGGGAGTCAAG+CGG	-	chr8.1:85747346-85747365	MS.gene34515:CDS	40.0%
!	GAACTCGAGGAACATTTTCA+AGG	-	chr8.1:85747095-85747114	MS.gene34515:CDS	40.0%
!!!	GAAGAGGCGTTTTAATGAAC+TGG	-	chr8.1:85747133-85747152	MS.gene34515:CDS	40.0%
	ACAGGTTTCTCCTTGACATC+TGG	+	chr8.1:85747374-85747393	None:intergenic	45.0%
	AGATGTCAAGGAGAAACCTG+TGG	-	chr8.1:85747373-85747392	MS.gene34515:CDS	45.0%
	CCTTGCTGCAGAGACTTCAA+AGG	-	chr8.1:85747246-85747265	MS.gene34515:CDS	50.0%
	GGCTGGAGAAATCCTTGAAG+AGG	-	chr8.1:85747117-85747136	MS.gene34515:CDS	50.0%
	TGTGGATGCTGTGTCCACTA+GGG	-	chr8.1:85747391-85747410	MS.gene34515:CDS	50.0%
!!	CCTTTGAAGTCTCTGCAGCA+AGG	+	chr8.1:85747249-85747268	None:intergenic	50.0%
!!	CGAGGAACATTTTCAAGGGC+TGG	-	chr8.1:85747100-85747119	MS.gene34515:CDS	50.0%
!!!	GGAAGCGGCTGTTTTTGCTA+AGG	-	chr8.1:85747217-85747236	MS.gene34515:CDS	50.0%
	CTGTGGATGCTGTGTCCACT+AGG	-	chr8.1:85747390-85747409	MS.gene34515:CDS	55.0%
	GATGTTGCCTTCAGCCCTAG+TGG	+	chr8.1:85747408-85747427	None:intergenic	55.0%
	TCAAGCGGCACCAGATGTCA+AGG	-	chr8.1:85747361-85747380	MS.gene34515:CDS	55.0%
!	CTAGTGGACACAGCATCCAC+AGG	+	chr8.1:85747392-85747411	None:intergenic	55.0%
	GCTGTGTCCACTAGGGCTGA+AGG	-	chr8.1:85747398-85747417	MS.gene34515:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	85746972	85747454	85746972	ID=MS.gene34515
chr8.1	mRNA	85746972	85747454	85746972	ID=MS.gene34515.t1;Parent=MS.gene34515
chr8.1	exon	85746972	85747454	85746972	ID=MS.gene34515.t1.exon1;Parent=MS.gene34515.t1
chr8.1	CDS	85746972	85747454	85746972	ID=cds.MS.gene34515.t1;Parent=MS.gene34515.t1

Gene Sequence

>MS.gene34515

ATGGAGGACAAGGAATCAATTGCTAAAATGATAGACTCTACATCTTCAAAGATACAACAGCTGCAGAAGGCATTTGCTGAACTTGAAAGTTACCGAGCTGTTACTCTTAACTTGAAATGGAAAGAACTCGAGGAACATTTTCAAGGGCTGGAGAAATCCTTGAAGAGGCGTTTTAATGAACTGGAAGACCAAGAGAAAGAGTTTGAAAACAAGACGAGGAAAGCTCGAGAAATGATAGAGAAACAGGAAGCGGCTGTTTTTGCTAAGGAGCAAGCCTTGCTGCAGAGACTTCAAAGGAAAAGAGATGCTTCTGTATTCACCATTGTAGATGCTCGGAAAAAATACAGGAAGGTTTCATCAAAGGACTTGGCTACTGTCTTTAATGGGAGTCAAGCGGCACCAGATGTCAAGGAGAAACCTGTGGATGCTGTGTCCACTAGGGCTGAAGGCAACATCGAAAATGCAAAACTTTCTCCTGCGAAT

Protein sequence

>MS.gene34515.t1

MEDKESIAKMIDSTSSKIQQLQKAFAELESYRAVTLNLKWKELEEHFQGLEKSLKRRFNELEDQEKEFENKTRKAREMIEKQEAAVFAKEQALLQRLQRKRDASVFTIVDARKKYRKVSSKDLATVFNGSQAAPDVKEKPVDAVSTRAEGNIENAKLSPAN