Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58919.t1 | XP_013458250.1 | 99.9 | 744 | 1 | 0 | 1 | 744 | 1 | 744 | 0.00E+00 | 1483.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58919.t1 | Q94AH6 | 81.8 | 740 | 132 | 1 | 5 | 744 | 2 | 738 | 0.0e+00 | 1244.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58919.t1 | A0A072USG4 | 99.9 | 744 | 1 | 0 | 1 | 744 | 1 | 744 | 0.0e+00 | 1483.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene58919 | 0.80079 | 1.22E-48 | -1.69E-46 |
| MS.gene049254 | MS.gene58919 | 0.859681 | 3.49E-63 | -1.69E-46 |
| MS.gene049283 | MS.gene58919 | 0.85036 | 1.79E-60 | -1.69E-46 |
| MS.gene049435 | MS.gene58919 | 0.825967 | 3.51E-54 | -1.69E-46 |
| MS.gene049452 | MS.gene58919 | 0.807624 | 4.62E-50 | -1.69E-46 |
| MS.gene049499 | MS.gene58919 | 0.846464 | 2.14E-59 | -1.69E-46 |
| MS.gene049801 | MS.gene58919 | 0.802404 | 5.70E-49 | -1.69E-46 |
| MS.gene049983 | MS.gene58919 | 0.833471 | 5.23E-56 | -1.69E-46 |
| MS.gene050073 | MS.gene58919 | 0.806559 | 7.76E-50 | -1.69E-46 |
| MS.gene050414 | MS.gene58919 | 0.800526 | 1.38E-48 | -1.69E-46 |
| MS.gene050559 | MS.gene58919 | 0.83234 | 1.00E-55 | -1.69E-46 |
| MS.gene050561 | MS.gene58919 | 0.831048 | 2.08E-55 | -1.69E-46 |
| MS.gene050562 | MS.gene58919 | 0.828463 | 8.86E-55 | -1.69E-46 |
| MS.gene050654 | MS.gene58919 | 0.817345 | 3.46E-52 | -1.69E-46 |
| MS.gene050759 | MS.gene58919 | 0.802086 | 6.63E-49 | -1.69E-46 |
| MS.gene050945 | MS.gene58919 | 0.818443 | 1.96E-52 | -1.69E-46 |
| MS.gene051198 | MS.gene58919 | 0.853883 | 1.78E-61 | -1.69E-46 |
| MS.gene051279 | MS.gene58919 | 0.808661 | 2.77E-50 | -1.69E-46 |
| MS.gene051332 | MS.gene58919 | 0.82758 | 1.45E-54 | -1.69E-46 |
| MS.gene051368 | MS.gene58919 | 0.801993 | 6.93E-49 | -1.69E-46 |
| MS.gene051597 | MS.gene58919 | 0.841088 | 5.87E-58 | -1.69E-46 |
| MS.gene051628 | MS.gene58919 | 0.807147 | 5.82E-50 | -1.69E-46 |
| MS.gene051699 | MS.gene58919 | 0.814292 | 1.66E-51 | -1.69E-46 |
| MS.gene051744 | MS.gene58919 | 0.822559 | 2.22E-53 | -1.69E-46 |
| MS.gene051800 | MS.gene58919 | 0.833035 | 6.72E-56 | -1.69E-46 |
| MS.gene052037 | MS.gene58919 | 0.82188 | 3.19E-53 | -1.69E-46 |
| MS.gene052108 | MS.gene58919 | 0.810066 | 1.39E-50 | -1.69E-46 |
| MS.gene052118 | MS.gene58919 | 0.808166 | 3.54E-50 | -1.69E-46 |
| MS.gene052239 | MS.gene58919 | -0.836511 | 8.96E-57 | -1.69E-46 |
| MS.gene052333 | MS.gene58919 | 0.803763 | 2.99E-49 | -1.69E-46 |
| MS.gene052534 | MS.gene58919 | 0.807827 | 4.18E-50 | -1.69E-46 |
| MS.gene052582 | MS.gene58919 | 0.848406 | 6.25E-60 | -1.69E-46 |
| MS.gene052584 | MS.gene58919 | 0.84698 | 1.54E-59 | -1.69E-46 |
| MS.gene052677 | MS.gene58919 | 0.803081 | 4.13E-49 | -1.69E-46 |
| MS.gene052855 | MS.gene58919 | 0.812506 | 4.10E-51 | -1.69E-46 |
| MS.gene052954 | MS.gene58919 | 0.827163 | 1.82E-54 | -1.69E-46 |
| MS.gene053079 | MS.gene58919 | 0.816503 | 5.35E-52 | -1.69E-46 |
| MS.gene05308 | MS.gene58919 | 0.829208 | 5.85E-55 | -1.69E-46 |
| MS.gene053226 | MS.gene58919 | 0.81478 | 1.29E-51 | -1.69E-46 |
| MS.gene053726 | MS.gene58919 | 0.814585 | 1.43E-51 | -1.69E-46 |
| MS.gene053931 | MS.gene58919 | 0.811132 | 8.16E-51 | -1.69E-46 |
| MS.gene053963 | MS.gene58919 | 0.834326 | 3.20E-56 | -1.69E-46 |
| MS.gene05415 | MS.gene58919 | 0.801311 | 9.56E-49 | -1.69E-46 |
| MS.gene054479 | MS.gene58919 | 0.836247 | 1.05E-56 | -1.69E-46 |
| MS.gene054576 | MS.gene58919 | 0.839558 | 1.47E-57 | -1.69E-46 |
| MS.gene054641 | MS.gene58919 | 0.808751 | 2.65E-50 | -1.69E-46 |
| MS.gene054651 | MS.gene58919 | 0.835919 | 1.27E-56 | -1.69E-46 |
| MS.gene054657 | MS.gene58919 | 0.825552 | 4.41E-54 | -1.69E-46 |
| MS.gene054892 | MS.gene58919 | 0.816409 | 5.61E-52 | -1.69E-46 |
| MS.gene054893 | MS.gene58919 | 0.821381 | 4.16E-53 | -1.69E-46 |
| MS.gene054957 | MS.gene58919 | 0.814725 | 1.33E-51 | -1.69E-46 |
| MS.gene055222 | MS.gene58919 | 0.803657 | 3.14E-49 | -1.69E-46 |
| MS.gene055586 | MS.gene58919 | 0.812832 | 3.48E-51 | -1.69E-46 |
| MS.gene055724 | MS.gene58919 | 0.802952 | 4.39E-49 | -1.69E-46 |
| MS.gene055841 | MS.gene58919 | 0.818004 | 2.46E-52 | -1.69E-46 |
| MS.gene056045 | MS.gene58919 | 0.804945 | 1.69E-49 | -1.69E-46 |
| MS.gene056050 | MS.gene58919 | 0.820448 | 6.82E-53 | -1.69E-46 |
| MS.gene056162 | MS.gene58919 | 0.836211 | 1.07E-56 | -1.69E-46 |
| MS.gene056174 | MS.gene58919 | 0.828894 | 6.97E-55 | -1.69E-46 |
| MS.gene056403 | MS.gene58919 | 0.81753 | 3.14E-52 | -1.69E-46 |
| MS.gene05659 | MS.gene58919 | 0.811402 | 7.13E-51 | -1.69E-46 |
| MS.gene056605 | MS.gene58919 | 0.808351 | 3.23E-50 | -1.69E-46 |
| MS.gene056776 | MS.gene58919 | 0.84261 | 2.33E-58 | -1.69E-46 |
| MS.gene056779 | MS.gene58919 | 0.801201 | 1.01E-48 | -1.69E-46 |
| MS.gene05686 | MS.gene58919 | 0.846206 | 2.51E-59 | -1.69E-46 |
| MS.gene05689 | MS.gene58919 | 0.815142 | 1.08E-51 | -1.69E-46 |
| MS.gene056973 | MS.gene58919 | 0.858475 | 8.03E-63 | -1.69E-46 |
| MS.gene057034 | MS.gene58919 | 0.808807 | 2.58E-50 | -1.69E-46 |
| MS.gene057208 | MS.gene58919 | 0.833676 | 4.65E-56 | -1.69E-46 |
| MS.gene057598 | MS.gene58919 | 0.809313 | 2.01E-50 | -1.69E-46 |
| MS.gene057869 | MS.gene58919 | 0.83351 | 5.12E-56 | -1.69E-46 |
| MS.gene057893 | MS.gene58919 | -0.81001 | 1.43E-50 | -1.69E-46 |
| MS.gene057919 | MS.gene58919 | 0.800551 | 1.37E-48 | -1.69E-46 |
| MS.gene058009 | MS.gene58919 | 0.806687 | 7.29E-50 | -1.69E-46 |
| MS.gene058010 | MS.gene58919 | 0.847923 | 8.50E-60 | -1.69E-46 |
| MS.gene058126 | MS.gene58919 | 0.81225 | 4.66E-51 | -1.69E-46 |
| MS.gene058159 | MS.gene58919 | 0.857582 | 1.48E-62 | -1.69E-46 |
| MS.gene058223 | MS.gene58919 | -0.809921 | 1.49E-50 | -1.69E-46 |
| MS.gene058279 | MS.gene58919 | 0.816794 | 4.60E-52 | -1.69E-46 |
| MS.gene058289 | MS.gene58919 | 0.809042 | 2.30E-50 | -1.69E-46 |
| MS.gene058292 | MS.gene58919 | 0.814565 | 1.44E-51 | -1.69E-46 |
| MS.gene058330 | MS.gene58919 | 0.81663 | 5.01E-52 | -1.69E-46 |
| MS.gene058548 | MS.gene58919 | 0.832408 | 9.61E-56 | -1.69E-46 |
| MS.gene058781 | MS.gene58919 | 0.81744 | 3.29E-52 | -1.69E-46 |
| MS.gene058979 | MS.gene58919 | 0.815257 | 1.01E-51 | -1.69E-46 |
| MS.gene059317 | MS.gene58919 | 0.803306 | 3.71E-49 | -1.69E-46 |
| MS.gene059439 | MS.gene58919 | 0.800138 | 1.66E-48 | -1.69E-46 |
| MS.gene05952 | MS.gene58919 | 0.810856 | 9.36E-51 | -1.69E-46 |
| MS.gene05985 | MS.gene58919 | -0.816571 | 5.16E-52 | -1.69E-46 |
| MS.gene060011 | MS.gene58919 | 0.811876 | 5.62E-51 | -1.69E-46 |
| MS.gene060365 | MS.gene58919 | 0.823442 | 1.38E-53 | -1.69E-46 |
| MS.gene060520 | MS.gene58919 | 0.824144 | 9.46E-54 | -1.69E-46 |
| MS.gene060521 | MS.gene58919 | 0.805116 | 1.56E-49 | -1.69E-46 |
| MS.gene06052 | MS.gene58919 | 0.821356 | 4.22E-53 | -1.69E-46 |
| MS.gene060777 | MS.gene58919 | 0.824245 | 8.96E-54 | -1.69E-46 |
| MS.gene060840 | MS.gene58919 | 0.815964 | 7.06E-52 | -1.69E-46 |
| MS.gene060933 | MS.gene58919 | 0.846258 | 2.43E-59 | -1.69E-46 |
| MS.gene060935 | MS.gene58919 | 0.837696 | 4.46E-57 | -1.69E-46 |
| MS.gene060937 | MS.gene58919 | 0.847244 | 1.31E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene34691 | MS.gene58919 | PPI |
| MS.gene35971 | MS.gene58919 | PPI |
| MS.gene004416 | MS.gene58919 | PPI |
| MS.gene25469 | MS.gene58919 | PPI |
| MS.gene58919 | MS.gene25471 | PPI |
| MS.gene58919 | MS.gene055864 | PPI |
| MS.gene58919 | MS.gene002729 | PPI |
| MS.gene96241 | MS.gene58919 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58919.t1 | MTR_4g119413 | 99.866 | 744 | 1 | 0 | 1 | 744 | 1 | 744 | 0.0 | 1541 |
| MS.gene58919.t1 | MTR_2g437390 | 93.127 | 742 | 51 | 0 | 3 | 744 | 1 | 742 | 0.0 | 1438 |
| MS.gene58919.t1 | MTR_7g108340 | 78.919 | 740 | 150 | 1 | 5 | 744 | 2 | 735 | 0.0 | 1239 |
| MS.gene58919.t1 | MTR_4g119413 | 99.488 | 586 | 3 | 0 | 1 | 586 | 1 | 586 | 0.0 | 1213 |
| MS.gene58919.t1 | MTR_8g056030 | 34.133 | 709 | 430 | 12 | 45 | 744 | 51 | 731 | 1.77e-129 | 402 |
| MS.gene58919.t1 | MTR_5g039880 | 33.604 | 738 | 437 | 15 | 34 | 744 | 21 | 732 | 1.16e-119 | 376 |
| MS.gene58919.t1 | MTR_2g019260 | 33.427 | 715 | 428 | 14 | 41 | 744 | 115 | 792 | 1.63e-119 | 377 |
| MS.gene58919.t1 | MTR_6g011700 | 32.615 | 742 | 432 | 13 | 12 | 744 | 23 | 705 | 1.67e-118 | 372 |
| MS.gene58919.t1 | MTR_6g011770 | 31.671 | 742 | 439 | 14 | 12 | 744 | 23 | 705 | 1.18e-112 | 357 |
| MS.gene58919.t1 | MTR_7g039690 | 33.153 | 739 | 415 | 17 | 34 | 744 | 21 | 708 | 8.49e-112 | 355 |
| MS.gene58919.t1 | MTR_7g039660 | 30.718 | 752 | 453 | 17 | 22 | 744 | 8 | 720 | 2.83e-106 | 341 |
| MS.gene58919.t1 | MTR_2g019260 | 31.865 | 681 | 418 | 13 | 41 | 710 | 115 | 760 | 9.35e-106 | 340 |
| MS.gene58919.t1 | MTR_1g040925 | 33.333 | 612 | 363 | 10 | 141 | 744 | 367 | 941 | 9.46e-92 | 307 |
| MS.gene58919.t1 | MTR_1g040925 | 25.182 | 274 | 187 | 4 | 94 | 365 | 102 | 359 | 2.32e-26 | 116 |
| MS.gene58919.t1 | MTR_5g039770 | 31.231 | 666 | 399 | 13 | 91 | 744 | 36 | 654 | 4.58e-88 | 291 |
| MS.gene58919.t1 | MTR_7g039790 | 30.254 | 747 | 449 | 18 | 11 | 744 | 30 | 717 | 3.20e-85 | 285 |
| MS.gene58919.t1 | MTR_1g040995 | 32.755 | 519 | 325 | 8 | 141 | 656 | 367 | 864 | 4.64e-79 | 271 |
| MS.gene58919.t1 | MTR_1g040995 | 25.182 | 274 | 187 | 4 | 94 | 365 | 102 | 359 | 1.93e-26 | 116 |
| MS.gene58919.t1 | MTR_7g445240 | 29.128 | 745 | 444 | 18 | 11 | 744 | 30 | 701 | 4.98e-77 | 262 |
| MS.gene58919.t1 | MTR_4g119490 | 56.000 | 200 | 88 | 0 | 9 | 208 | 5 | 204 | 1.17e-71 | 233 |
| MS.gene58919.t1 | MTR_4g119450 | 55.392 | 204 | 87 | 2 | 7 | 208 | 3 | 204 | 3.84e-69 | 226 |
| MS.gene58919.t1 | MTR_4g119930 | 53.365 | 208 | 94 | 1 | 1 | 208 | 1 | 205 | 4.30e-63 | 209 |
| MS.gene58919.t1 | MTR_4g119422 | 52.036 | 221 | 98 | 3 | 9 | 224 | 3 | 220 | 4.71e-63 | 215 |
| MS.gene58919.t1 | MTR_4g119930 | 58.491 | 159 | 63 | 1 | 1 | 159 | 1 | 156 | 1.58e-51 | 177 |
| MS.gene58919.t1 | MTR_5g040040 | 34.675 | 323 | 172 | 6 | 429 | 744 | 296 | 586 | 2.24e-45 | 172 |
| MS.gene58919.t1 | MTR_4g119930 | 51.592 | 157 | 76 | 0 | 52 | 208 | 1 | 157 | 2.61e-43 | 154 |
| MS.gene58919.t1 | MTR_5g039890 | 25.053 | 471 | 300 | 13 | 12 | 448 | 23 | 474 | 4.26e-38 | 149 |
| MS.gene58919.t1 | MTR_1g040965 | 40.826 | 218 | 122 | 3 | 534 | 744 | 1 | 218 | 2.68e-37 | 139 |
| MS.gene58919.t1 | MTR_3g115680 | 36.598 | 194 | 109 | 4 | 1 | 189 | 1 | 185 | 5.69e-33 | 126 |
| MS.gene58919.t1 | MTR_7g039440 | 26.645 | 304 | 198 | 6 | 84 | 382 | 11 | 294 | 9.93e-32 | 126 |
| MS.gene58919.t1 | MTR_5g040180 | 26.347 | 334 | 213 | 7 | 61 | 382 | 46 | 358 | 2.72e-31 | 126 |
| MS.gene58919.t1 | MTR_7g039680 | 23.810 | 378 | 242 | 9 | 12 | 387 | 23 | 356 | 5.11e-30 | 122 |
| MS.gene58919.t1 | MTR_7g039430 | 28.571 | 238 | 164 | 4 | 84 | 319 | 75 | 308 | 1.29e-27 | 115 |
| MS.gene58919.t1 | MTR_5g040190 | 28.509 | 228 | 157 | 4 | 94 | 319 | 85 | 308 | 1.52e-27 | 115 |
| MS.gene58919.t1 | MTR_4g131910 | 57.831 | 83 | 34 | 1 | 3 | 85 | 1 | 82 | 1.91e-23 | 100 |
| MS.gene58919.t1 | MTR_4g131905 | 42.574 | 101 | 46 | 4 | 47 | 147 | 57 | 145 | 1.67e-14 | 71.6 |
| MS.gene58919.t1 | MTR_7g039770 | 45.122 | 82 | 45 | 0 | 663 | 744 | 115 | 196 | 2.70e-14 | 72.8 |
| MS.gene58919.t1 | MTR_7g445230 | 45.122 | 82 | 45 | 0 | 663 | 744 | 115 | 196 | 2.70e-14 | 72.8 |
| MS.gene58919.t1 | MTR_4g082150 | 28.022 | 182 | 121 | 2 | 425 | 598 | 377 | 556 | 6.16e-12 | 69.7 |
| MS.gene58919.t1 | MTR_4g082150 | 26.923 | 182 | 123 | 2 | 425 | 598 | 553 | 732 | 8.55e-12 | 69.3 |
| MS.gene58919.t1 | MTR_2g018360 | 74.359 | 39 | 10 | 0 | 620 | 658 | 30 | 68 | 2.47e-11 | 60.8 |
| MS.gene58919.t1 | MTR_4g082265 | 26.923 | 182 | 123 | 2 | 425 | 598 | 553 | 732 | 2.88e-11 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58919.t1 | AT4G02570 | 81.757 | 740 | 132 | 1 | 5 | 744 | 2 | 738 | 0.0 | 1295 |
| MS.gene58919.t1 | AT4G02570 | 81.757 | 740 | 132 | 1 | 5 | 744 | 2 | 738 | 0.0 | 1295 |
| MS.gene58919.t1 | AT4G02570 | 81.757 | 740 | 132 | 1 | 5 | 744 | 2 | 738 | 0.0 | 1295 |
| MS.gene58919.t1 | AT4G02570 | 81.757 | 740 | 132 | 1 | 5 | 744 | 2 | 738 | 0.0 | 1295 |
| MS.gene58919.t1 | AT1G02980 | 66.801 | 744 | 243 | 4 | 3 | 744 | 1 | 742 | 0.0 | 1052 |
| MS.gene58919.t1 | AT1G02980 | 66.801 | 744 | 243 | 4 | 3 | 744 | 1 | 742 | 0.0 | 1052 |
| MS.gene58919.t1 | AT1G43140 | 65.946 | 740 | 221 | 3 | 6 | 744 | 12 | 721 | 0.0 | 1011 |
| MS.gene58919.t1 | AT1G59790 | 56.771 | 384 | 145 | 3 | 3 | 384 | 6 | 370 | 2.54e-152 | 448 |
| MS.gene58919.t1 | AT1G59790 | 56.720 | 372 | 140 | 3 | 3 | 372 | 6 | 358 | 8.50e-147 | 434 |
| MS.gene58919.t1 | AT1G26830 | 34.713 | 749 | 431 | 14 | 12 | 744 | 26 | 732 | 9.11e-135 | 416 |
| MS.gene58919.t1 | AT1G69670 | 34.274 | 709 | 429 | 13 | 45 | 744 | 52 | 732 | 3.93e-129 | 401 |
| MS.gene58919.t1 | AT1G69670 | 34.274 | 709 | 429 | 13 | 45 | 744 | 52 | 732 | 3.93e-129 | 401 |
| MS.gene58919.t1 | AT5G46210 | 34.637 | 716 | 418 | 17 | 41 | 744 | 115 | 792 | 5.85e-112 | 358 |
| MS.gene58919.t1 | AT1G59800 | 54.918 | 244 | 106 | 1 | 9 | 252 | 7 | 246 | 4.00e-89 | 280 |
Find 141 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGTTCTGACCACTGGTTTC+TGG | 0.096559 | 8.3:+7522600 | MS.gene58919:CDS |
| TTCTCGTTTCTTTCACTATT+TGG | 0.127323 | 8.3:+7518760 | MS.gene58919:CDS |
| GCTAAGTACTTGAACATATT+TGG | 0.130944 | 8.3:-7523731 | None:intergenic |
| TTAGATATATTTGTTGAAAT+TGG | 0.149900 | 8.3:+7519223 | MS.gene58919:CDS |
| AATGACTCAGTTAAACTTAT+TGG | 0.163771 | 8.3:+7523103 | MS.gene58919:CDS |
| CATGTAGCTCAATCACTTTC+CGG | 0.271035 | 8.3:-7520682 | None:intergenic |
| TGAAATTGGAATGGGGAAAA+TGG | 0.276258 | 8.3:+7519237 | MS.gene58919:CDS |
| TATGAAGAGTCGGAAAGTTT+TGG | 0.279840 | 8.3:+7523478 | MS.gene58919:CDS |
| TAAAATCCCATCCTTGTTCT+AGG | 0.294855 | 8.3:-7516485 | None:intergenic |
| AAACTTCCCACTTATGTTAC+AGG | 0.307020 | 8.3:-7522849 | None:intergenic |
| ATTCTCGAAAGGCTTCAAAT+TGG | 0.308428 | 8.3:+7519314 | MS.gene58919:CDS |
| TGTGGAAAAGAGTGTGATTC+TGG | 0.331752 | 8.3:-7520736 | None:intergenic |
| AATCAGGACAAGAATCTTCT+AGG | 0.335743 | 8.3:-7519339 | None:intergenic |
| TAGTGAGCCAAAGTTATTAG+AGG | 0.338740 | 8.3:+7519515 | MS.gene58919:CDS |
| GCTTAAGACGGGAGAAAGAT+AGG | 0.347462 | 8.3:+7519469 | MS.gene58919:CDS |
| GCAGTTGGGTCGCATGTTTA+AGG | 0.348970 | 8.3:+7523532 | MS.gene58919:CDS |
| GACCATTCCTTCCATCTTTG+AGG | 0.353692 | 8.3:-7522376 | None:intergenic |
| GTAACTGTTAAGTCTATTCC+AGG | 0.375194 | 8.3:-7522578 | None:intergenic |
| TTTGATTTCTCGGGATTACT+TGG | 0.380388 | 8.3:+7523691 | MS.gene58919:CDS |
| ACTTACATGGATTTACTCCT+TGG | 0.389503 | 8.3:+7522823 | MS.gene58919:CDS |
| TACAGCATCATGTAATCTTC+AGG | 0.394192 | 8.3:-7516570 | None:intergenic |
| GTTGGCTTGCTTCCGTGATT+TGG | 0.395108 | 8.3:+7518832 | MS.gene58919:CDS |
| CTTCCACCTCTTAATGAAGT+TGG | 0.401442 | 8.3:+7518809 | MS.gene58919:CDS |
| TTCCACCTCTTAATGAAGTT+GGG | 0.402385 | 8.3:+7518810 | MS.gene58919:CDS |
| TGCTTTACTTAGAGATGATA+AGG | 0.405401 | 8.3:+7519802 | MS.gene58919:CDS |
| AAGACTTGTCAAGGATGTTC+AGG | 0.408359 | 8.3:+7519919 | MS.gene58919:CDS |
| TGTGATAACATTCTTAAGAA+AGG | 0.408911 | 8.3:+7520916 | MS.gene58919:CDS |
| TTCTAAAATACCCCGTGGCT+TGG | 0.411928 | 8.3:+7519947 | MS.gene58919:CDS |
| ATATTTGTTGAAATTGGAAT+GGG | 0.416286 | 8.3:+7519229 | MS.gene58919:CDS |
| TCTATTCCAGGGTCTGCATT+AGG | 0.419807 | 8.3:-7522566 | None:intergenic |
| TCTCAATCTTCCTGCAGAAA+TGG | 0.424664 | 8.3:+7522643 | MS.gene58919:CDS |
| TATTGGATGAAGATGTCATT+AGG | 0.425424 | 8.3:+7523120 | MS.gene58919:CDS |
| ACCTCTTAATGAAGTTGGGT+TGG | 0.429517 | 8.3:+7518814 | MS.gene58919:CDS |
| AGATGCAGCTAGTAACAAAA+AGG | 0.437346 | 8.3:+7520170 | MS.gene58919:CDS |
| AAGCTCAAGAACATTCTTGA+AGG | 0.438292 | 8.3:+7516525 | MS.gene58919:CDS |
| CAGGTTTACAAGGAACTACA+TGG | 0.439895 | 8.3:+7519034 | MS.gene58919:intron |
| GGGCTATCAACAGTTAGTTA+TGG | 0.441591 | 8.3:+7523499 | MS.gene58919:CDS |
| AAGAGTGTGATTCTGGAAAC+AGG | 0.442171 | 8.3:-7520729 | None:intergenic |
| ACTTGTTTGCCGAGTTCTAC+AGG | 0.445269 | 8.3:+7521161 | MS.gene58919:CDS |
| AGATCAAAAGATTTATAACT+AGG | 0.445948 | 8.3:-7522623 | None:intergenic |
| TGCTTAAATATACTAGAAAC+AGG | 0.455563 | 8.3:-7519972 | None:intergenic |
| GAACAAACCTCTAATAACTT+TGG | 0.461535 | 8.3:-7519522 | None:intergenic |
| TTCTCATCCACAGGAGGTAG+AGG | 0.465842 | 8.3:-7523389 | None:intergenic |
| TATGGAGTGCGTTGAGCAGT+TGG | 0.466660 | 8.3:+7523517 | MS.gene58919:CDS |
| GATTGAAGATTTGATTTCTC+GGG | 0.468239 | 8.3:+7523682 | MS.gene58919:CDS |
| ATGTCTACAGGCAGAGAAAA+GGG | 0.468308 | 8.3:+7520553 | MS.gene58919:intron |
| CTTACATGGATTTACTCCTT+GGG | 0.469426 | 8.3:+7522824 | MS.gene58919:CDS |
| GCCAACCCAACTTCATTAAG+AGG | 0.469591 | 8.3:-7518815 | None:intergenic |
| TATATTTGTTGAAATTGGAA+TGG | 0.470308 | 8.3:+7519228 | MS.gene58919:CDS |
| ATTAAATTCAAAATAATCAG+TGG | 0.477551 | 8.3:-7523213 | None:intergenic |
| ATCCAGATTACCATTTCTGC+AGG | 0.478678 | 8.3:-7522653 | None:intergenic |
| TGTTTCTAGTATATTTAAGC+AGG | 0.479126 | 8.3:+7519974 | MS.gene58919:CDS |
| AATGTCTACAGGCAGAGAAA+AGG | 0.480638 | 8.3:+7520552 | MS.gene58919:intron |
| CATACCTTGAGCATATAATC+AGG | 0.483000 | 8.3:-7519355 | None:intergenic |
| TAAGAGGTGGAAGTGACCTC+CGG | 0.485058 | 8.3:-7518799 | None:intergenic |
| GCGCACAATTGAGGCATCAA+TGG | 0.485374 | 8.3:-7523453 | None:intergenic |
| AAAGTATAAGATTCTTATCA+AGG | 0.493353 | 8.3:+7523166 | MS.gene58919:CDS |
| TCAGGACTGAATTGAGGCTC+AGG | 0.498314 | 8.3:-7516552 | None:intergenic |
| GGATTGAAGATTTGATTTCT+CGG | 0.500636 | 8.3:+7523681 | MS.gene58919:CDS |
| TTATTATTTACAGGTTTACA+AGG | 0.507125 | 8.3:+7519024 | MS.gene58919:intron |
| AATTGAAGAAACTCTTGAAA+AGG | 0.507211 | 8.3:+7520966 | MS.gene58919:CDS |
| GGAGAATCAAACTAGCTTTG+AGG | 0.511793 | 8.3:+7522526 | MS.gene58919:CDS |
| ACAGTTACAGTTCTGACCAC+TGG | 0.512956 | 8.3:+7522593 | MS.gene58919:CDS |
| TATCAGCATGTTACCACTGA+AGG | 0.513329 | 8.3:+7520123 | MS.gene58919:intron |
| AGCGGCTGAGAATAGTCATT+AGG | 0.516656 | 8.3:-7516737 | None:intergenic |
| CTTCATAATGCGCACAATTG+AGG | 0.520124 | 8.3:-7523462 | None:intergenic |
| TTCTGCTTATTATTCTCGAA+AGG | 0.520286 | 8.3:+7519303 | MS.gene58919:CDS |
| TATTGTTGGTACGCAGGAGC+AGG | 0.520932 | 8.3:+7520577 | MS.gene58919:CDS |
| ATGGAGTGCGTTGAGCAGTT+GGG | 0.521583 | 8.3:+7523518 | MS.gene58919:CDS |
| GCAGAGAAAAGGGATATTGT+TGG | 0.521890 | 8.3:+7520563 | MS.gene58919:CDS |
| TCAGGAAGAAGCTTGCTCGC+AGG | 0.522136 | 8.3:+7522271 | MS.gene58919:intron |
| GATCATATAGGCTCTTAAAG+AGG | 0.526409 | 8.3:+7520828 | MS.gene58919:intron |
| CAAAGTTCACTGACAAAATG+AGG | 0.526696 | 8.3:+7523237 | MS.gene58919:CDS |
| CAAAGCACAGAAAACTTACA+TGG | 0.526940 | 8.3:+7522810 | MS.gene58919:CDS |
| TGGACCGCTACTTCATTGCC+CGG | 0.528357 | 8.3:+7518780 | MS.gene58919:CDS |
| TTGGGTACCTGTAACATAAG+TGG | 0.530949 | 8.3:+7522842 | MS.gene58919:CDS |
| TACAGATTTGACGCTGGCAA+AGG | 0.535172 | 8.3:+7522505 | MS.gene58919:CDS |
| TACGAGAACTTGTAAAGCGA+TGG | 0.537030 | 8.3:+7518708 | MS.gene58919:CDS |
| GCTTGTTGTCACAACCTACC+AGG | 0.542445 | 8.3:+7522889 | MS.gene58919:CDS |
| TTGTCCTGATTATATGCTCA+AGG | 0.542877 | 8.3:+7519351 | MS.gene58919:CDS |
| GGCTGAGGAGTGCTTAAGAC+GGG | 0.543587 | 8.3:+7519458 | MS.gene58919:intron |
| CTATAGACCTAGAACAAGGA+TGG | 0.545135 | 8.3:+7516478 | MS.gene58919:CDS |
| ATCCCGAGTGCTCTTTCTCA+AGG | 0.548621 | 8.3:-7519775 | None:intergenic |
| GCAACATGTCAATGAGTGAA+AGG | 0.550034 | 8.3:+7516451 | None:intergenic |
| TACCTCAAAGATGGAAGGAA+TGG | 0.555295 | 8.3:+7522374 | MS.gene58919:CDS |
| GCTCCTTGAGAAAGAGCACT+CGG | 0.563609 | 8.3:+7519772 | MS.gene58919:CDS |
| GCATGTTACCACTGAAGGCA+TGG | 0.566747 | 8.3:+7520128 | MS.gene58919:intron |
| GTAGATTCCTCTACCTCCTG+TGG | 0.567732 | 8.3:+7523382 | MS.gene58919:intron |
| TGACAAAATGAGGAGGATCA+AGG | 0.568312 | 8.3:+7523247 | MS.gene58919:CDS |
| TGGACAGTTTACCTCAAAGA+TGG | 0.570096 | 8.3:+7522365 | MS.gene58919:CDS |
| ACTTTGCACATGACAAAGAG+TGG | 0.575661 | 8.3:-7523148 | None:intergenic |
| TGAAGAGTACATCGTGTCAA+CGG | 0.578165 | 8.3:+7516784 | MS.gene58919:CDS |
| AAGAGGTGGAAGTGACCTCC+GGG | 0.579880 | 8.3:-7518798 | None:intergenic |
| AACAGGAGTTCACATATGCC+AGG | 0.580301 | 8.3:-7520712 | None:intergenic |
| TAATCTTCAGGACTGAATTG+AGG | 0.582140 | 8.3:-7516558 | None:intergenic |
| CTTCAAAATCATTCTCATAG+TGG | 0.584709 | 8.3:-7519261 | None:intergenic |
| CTTAAGACGGGAGAAAGATA+GGG | 0.586296 | 8.3:+7519470 | MS.gene58919:CDS |
| AAGGGATATTGTTGGTACGC+AGG | 0.588845 | 8.3:+7520571 | MS.gene58919:CDS |
| ATGGGCGAACCATAAAATCA+TGG | 0.588851 | 8.3:+7518727 | MS.gene58919:CDS |
| TATTTGTTGAAATTGGAATG+GGG | 0.591338 | 8.3:+7519230 | MS.gene58919:CDS |
| TGGGTACCTGTAACATAAGT+GGG | 0.595127 | 8.3:+7522843 | MS.gene58919:CDS |
| TTGGCTCACTACTAGAGTGC+AGG | 0.595754 | 8.3:-7519503 | None:intergenic |
| CTCCTTGAGAAAGAGCACTC+GGG | 0.598786 | 8.3:+7519773 | MS.gene58919:CDS |
| TTAAATTCAAAATAATCAGT+GGG | 0.599039 | 8.3:-7523212 | None:intergenic |
| ACGAGAACTTGTAAAGCGAT+GGG | 0.601465 | 8.3:+7518709 | MS.gene58919:CDS |
| TATAGACCTAGAACAAGGAT+GGG | 0.601596 | 8.3:+7516479 | MS.gene58919:CDS |
| AGAAACAGGATCCAAGCCAC+GGG | 0.607688 | 8.3:-7519958 | None:intergenic |
| AAAGATTGATCAAGAACGCG+AGG | 0.608317 | 8.3:+7519165 | MS.gene58919:intron |
| CACCAACGCCATGCCTTCAG+TGG | 0.612973 | 8.3:-7520136 | None:intergenic |
| GGCTGAGAATAGTCATTAGG+AGG | 0.613345 | 8.3:-7516734 | None:intergenic |
| AGGCTGAGGAGTGCTTAAGA+CGG | 0.615169 | 8.3:+7519457 | MS.gene58919:intron |
| ACCATAAAATCATGGTGAGG+TGG | 0.616474 | 8.3:+7518735 | MS.gene58919:CDS |
| ACTGTAACATTACCAAATCA+CGG | 0.617141 | 8.3:-7518844 | None:intergenic |
| TCCTTGTACTTGTCATACAG+CGG | 0.620605 | 8.3:-7516755 | None:intergenic |
| GGTAAGTGATATAACCTGGT+AGG | 0.620880 | 8.3:-7522903 | None:intergenic |
| CAGTTTACCTCAAAGATGGA+AGG | 0.621254 | 8.3:+7522369 | MS.gene58919:CDS |
| TAGAAACAGGATCCAAGCCA+CGG | 0.624942 | 8.3:-7519959 | None:intergenic |
| GATAACATTCTTAAGAAAGG+AGG | 0.627547 | 8.3:+7520919 | MS.gene58919:CDS |
| AATACTCCTAATGCAGACCC+TGG | 0.628370 | 8.3:+7522560 | MS.gene58919:CDS |
| TTCAGTGTGAAGACTTGTCA+AGG | 0.628529 | 8.3:+7519910 | MS.gene58919:intron |
| TTGTGCGCATTATGAAGAGT+CGG | 0.630462 | 8.3:+7523468 | MS.gene58919:CDS |
| GCCGCTGTATGACAAGTACA+AGG | 0.634517 | 8.3:+7516754 | MS.gene58919:CDS |
| AGAATAACTCAGTCTATCAG+AGG | 0.636581 | 8.3:-7523075 | None:intergenic |
| ACTTATGTTACAGGTACCCA+AGG | 0.636697 | 8.3:-7522840 | None:intergenic |
| ACCTCCGGGCAATGAAGTAG+CGG | 0.640019 | 8.3:-7518784 | None:intergenic |
| AACCCAACTTCATTAAGAGG+TGG | 0.641652 | 8.3:-7518812 | None:intergenic |
| AAATTGAAGCAACAGTGTGG+TGG | 0.641731 | 8.3:+7522345 | MS.gene58919:CDS |
| TGAGCTACATGACAAGTACC+TGG | 0.651511 | 8.3:+7520694 | MS.gene58919:CDS |
| TACCACTGAAGGCATGGCGT+TGG | 0.656008 | 8.3:+7520134 | MS.gene58919:CDS |
| AAGATTGATCAAGAACGCGA+GGG | 0.661144 | 8.3:+7519166 | MS.gene58919:intron |
| AGTTCACTGACAAAATGAGG+AGG | 0.668852 | 8.3:+7523240 | MS.gene58919:CDS |
| CCAGGTTACAGATTTGACGC+TGG | 0.681048 | 8.3:+7522499 | MS.gene58919:intron |
| AGGTTTACAAGGAACTACAT+GGG | 0.683050 | 8.3:+7519035 | MS.gene58919:intron |
| GCTCGCTTATATTAGTGACA+AGG | 0.684698 | 8.3:+7521138 | MS.gene58919:CDS |
| TAACTGTTAAGTCTATTCCA+GGG | 0.696441 | 8.3:-7522577 | None:intergenic |
| ACCGCTACTTCATTGCCCGG+AGG | 0.702395 | 8.3:+7518783 | MS.gene58919:CDS |
| ACAAAATTGAAGCAACAGTG+TGG | 0.705779 | 8.3:+7522342 | MS.gene58919:CDS |
| AAAACTATAGACCTAGAACA+AGG | 0.706435 | 8.3:+7516474 | MS.gene58919:CDS |
| CGAACCATAAAATCATGGTG+AGG | 0.707704 | 8.3:+7518732 | MS.gene58919:CDS |
| ATAACTAGGCCAGAAACCAG+TGG | 0.715997 | 8.3:-7522609 | None:intergenic |
| GAAACAGGATCCAAGCCACG+GGG | 0.759466 | 8.3:-7519957 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 7516456 | 7523757 | 7516456 | ID=MS.gene58919 |
| chr8.3 | mRNA | 7516456 | 7523757 | 7516456 | ID=MS.gene58919.t1;Parent=MS.gene58919 |
| chr8.3 | exon | 7516456 | 7516595 | 7516456 | ID=MS.gene58919.t1.exon1;Parent=MS.gene58919.t1 |
| chr8.3 | CDS | 7516456 | 7516595 | 7516456 | ID=cds.MS.gene58919.t1;Parent=MS.gene58919.t1 |
| chr8.3 | exon | 7516706 | 7516805 | 7516706 | ID=MS.gene58919.t1.exon2;Parent=MS.gene58919.t1 |
| chr8.3 | CDS | 7516706 | 7516805 | 7516706 | ID=cds.MS.gene58919.t1;Parent=MS.gene58919.t1 |
| chr8.3 | exon | 7518668 | 7518853 | 7518668 | ID=MS.gene58919.t1.exon3;Parent=MS.gene58919.t1 |
| chr8.3 | CDS | 7518668 | 7518853 | 7518668 | ID=cds.MS.gene58919.t1;Parent=MS.gene58919.t1 |
| chr8.3 | exon | 7519037 | 7519084 | 7519037 | ID=MS.gene58919.t1.exon4;Parent=MS.gene58919.t1 |
| chr8.3 | CDS | 7519037 | 7519084 | 7519037 | ID=cds.MS.gene58919.t1;Parent=MS.gene58919.t1 |
| chr8.3 | exon | 7519169 | 7519372 | 7519169 | ID=MS.gene58919.t1.exon5;Parent=MS.gene58919.t1 |
| chr8.3 | CDS | 7519169 | 7519372 | 7519169 | ID=cds.MS.gene58919.t1;Parent=MS.gene58919.t1 |
| chr8.3 | exon | 7519459 | 7519536 | 7519459 | ID=MS.gene58919.t1.exon6;Parent=MS.gene58919.t1 |
| chr8.3 | CDS | 7519459 | 7519536 | 7519459 | ID=cds.MS.gene58919.t1;Parent=MS.gene58919.t1 |
| chr8.3 | exon | 7519734 | 7519823 | 7519734 | ID=MS.gene58919.t1.exon7;Parent=MS.gene58919.t1 |
| chr8.3 | CDS | 7519734 | 7519823 | 7519734 | ID=cds.MS.gene58919.t1;Parent=MS.gene58919.t1 |
| chr8.3 | exon | 7519915 | 7519995 | 7519915 | ID=MS.gene58919.t1.exon8;Parent=MS.gene58919.t1 |
| chr8.3 | CDS | 7519915 | 7519995 | 7519915 | ID=cds.MS.gene58919.t1;Parent=MS.gene58919.t1 |
| chr8.3 | exon | 7520129 | 7520191 | 7520129 | ID=MS.gene58919.t1.exon9;Parent=MS.gene58919.t1 |
| chr8.3 | CDS | 7520129 | 7520191 | 7520129 | ID=cds.MS.gene58919.t1;Parent=MS.gene58919.t1 |
| chr8.3 | exon | 7520563 | 7520598 | 7520563 | ID=MS.gene58919.t1.exon10;Parent=MS.gene58919.t1 |
| chr8.3 | CDS | 7520563 | 7520598 | 7520563 | ID=cds.MS.gene58919.t1;Parent=MS.gene58919.t1 |
| chr8.3 | exon | 7520674 | 7520760 | 7520674 | ID=MS.gene58919.t1.exon11;Parent=MS.gene58919.t1 |
| chr8.3 | CDS | 7520674 | 7520760 | 7520674 | ID=cds.MS.gene58919.t1;Parent=MS.gene58919.t1 |
| chr8.3 | exon | 7520838 | 7520987 | 7520838 | ID=MS.gene58919.t1.exon12;Parent=MS.gene58919.t1 |
| chr8.3 | CDS | 7520838 | 7520987 | 7520838 | ID=cds.MS.gene58919.t1;Parent=MS.gene58919.t1 |
| chr8.3 | exon | 7521127 | 7521182 | 7521127 | ID=MS.gene58919.t1.exon13;Parent=MS.gene58919.t1 |
| chr8.3 | CDS | 7521127 | 7521182 | 7521127 | ID=cds.MS.gene58919.t1;Parent=MS.gene58919.t1 |
| chr8.3 | exon | 7522275 | 7522395 | 7522275 | ID=MS.gene58919.t1.exon14;Parent=MS.gene58919.t1 |
| chr8.3 | CDS | 7522275 | 7522395 | 7522275 | ID=cds.MS.gene58919.t1;Parent=MS.gene58919.t1 |
| chr8.3 | exon | 7522503 | 7522664 | 7522503 | ID=MS.gene58919.t1.exon15;Parent=MS.gene58919.t1 |
| chr8.3 | CDS | 7522503 | 7522664 | 7522503 | ID=cds.MS.gene58919.t1;Parent=MS.gene58919.t1 |
| chr8.3 | exon | 7522767 | 7522910 | 7522767 | ID=MS.gene58919.t1.exon16;Parent=MS.gene58919.t1 |
| chr8.3 | CDS | 7522767 | 7522910 | 7522767 | ID=cds.MS.gene58919.t1;Parent=MS.gene58919.t1 |
| chr8.3 | exon | 7523050 | 7523268 | 7523050 | ID=MS.gene58919.t1.exon17;Parent=MS.gene58919.t1 |
| chr8.3 | CDS | 7523050 | 7523268 | 7523050 | ID=cds.MS.gene58919.t1;Parent=MS.gene58919.t1 |
| chr8.3 | exon | 7523386 | 7523553 | 7523386 | ID=MS.gene58919.t1.exon18;Parent=MS.gene58919.t1 |
| chr8.3 | CDS | 7523386 | 7523553 | 7523386 | ID=cds.MS.gene58919.t1;Parent=MS.gene58919.t1 |
| chr8.3 | exon | 7523656 | 7523757 | 7523656 | ID=MS.gene58919.t1.exon19;Parent=MS.gene58919.t1 |
| chr8.3 | CDS | 7523656 | 7523757 | 7523656 | ID=cds.MS.gene58919.t1;Parent=MS.gene58919.t1 |
| Gene Sequence |
| Protein sequence |
