AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43039


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43039.t1 MTR_2g086480 93.007 143 10 0 1 143 1 143 2.97e-89 262
MS.gene43039.t1 MTR_1g059990 51.613 62 29 1 71 132 49 109 9.97e-15 66.2
MS.gene43039.t1 MTR_7g104540 42.391 92 50 2 53 144 82 170 3.44e-14 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43039.t1 AT3G44450 48.101 79 33 2 73 145 38 114 1.00e-18 76.6
MS.gene43039.t1 AT3G52740 46.479 71 36 1 74 144 65 133 1.06e-16 72.0

Find 33 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTTACTCTCTTCCTCTT+TGG 0.196605 2.4:+13772183 None:intergenic
ATTTACGTACATCAGCAATA+AGG 0.310452 2.4:+13771969 None:intergenic
AGGTTAAAGTACCTGAAAAC+TGG 0.340594 2.4:-13772087 MS.gene43039:CDS
ATAATAATGAAGTTGGAATA+TGG 0.362191 2.4:+13772290 None:intergenic
AAATCAAGGTCACAAAGATT+AGG 0.365491 2.4:-13771950 MS.gene43039:CDS
TGCTAGGGTAGAGGAAATTA+CGG 0.392659 2.4:-13772152 MS.gene43039:CDS
AATCAAGGTCACAAAGATTA+GGG 0.392972 2.4:-13771949 MS.gene43039:CDS
GAGGCATAGGGAAGAGATGA+AGG 0.397356 2.4:-13772113 MS.gene43039:CDS
TGAAGGGATAATAATGAAGT+TGG 0.401976 2.4:+13772283 None:intergenic
TTTACGTACATCAGCAATAA+GGG 0.413998 2.4:+13771970 None:intergenic
AGGAACAAAAGCTGAAAGAA+TGG 0.465707 2.4:-13772060 MS.gene43039:CDS
GCTAGGGTAGAGGAAATTAC+GGG 0.480315 2.4:-13772151 MS.gene43039:CDS
GGAACAAAAGCTGAAAGAAT+GGG 0.483468 2.4:-13772059 MS.gene43039:CDS
AAGTTGGAATATGGCAAGTA+GGG 0.487487 2.4:+13772299 None:intergenic
GAAGTTGGAATATGGCAAGT+AGG 0.507037 2.4:+13772298 None:intergenic
ATAAATTAAGAAACTAGGTA+TGG 0.512293 2.4:+13771924 None:intergenic
GTAAAGAGAATGGTACTGCT+AGG 0.520978 2.4:-13772168 MS.gene43039:CDS
GAATGGTACTGCTAGGGTAG+AGG 0.528884 2.4:-13772161 MS.gene43039:CDS
GAGGAAGAGAGTAAAGAGAA+TGG 0.530342 2.4:-13772178 MS.gene43039:CDS
GTGAGAGGTTGAAGAGGCAT+AGG 0.534191 2.4:-13772126 MS.gene43039:CDS
TAAAGAGAATGGTACTGCTA+GGG 0.542678 2.4:-13772167 MS.gene43039:CDS
TTGCTGATGTACGTAAATCA+AGG 0.545508 2.4:-13771964 MS.gene43039:CDS
AGGAAATTACGGGGCGTGAG+AGG 0.563528 2.4:-13772141 MS.gene43039:CDS
TAGGGAAGAGATGAAGGGAA+AGG 0.571304 2.4:-13772107 MS.gene43039:CDS
GGTTAAAGTACCTGAAAACT+GGG 0.585046 2.4:-13772086 MS.gene43039:CDS
AGGCATAGGGAAGAGATGAA+GGG 0.595639 2.4:-13772112 MS.gene43039:CDS
TGAGAGGTTGAAGAGGCATA+GGG 0.598492 2.4:-13772125 MS.gene43039:CDS
TGATGATGAAAAGGCCAAAG+AGG 0.600501 2.4:-13772197 MS.gene43039:CDS
AGTACCTGAAAACTGGGAGA+AGG 0.624441 2.4:-13772080 MS.gene43039:CDS
CGGGGCGTGAGAGGTTGAAG+AGG 0.628226 2.4:-13772132 MS.gene43039:CDS
GCATTAACAATCAATGCATG+AGG 0.666520 2.4:+13772001 None:intergenic
TGACTCATAACATCTCAACA+TGG 0.722472 2.4:-13772333 MS.gene43039:CDS
CTAGGGTAGAGGAAATTACG+GGG 0.760541 2.4:-13772150 MS.gene43039:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATAATGAAGTTGGAATA+TGG + chr2.4:13772002-13772021 None:intergenic 20.0%
!! CTTGTAATCATTTTGTTGAA+GGG + chr2.4:13772025-13772044 None:intergenic 25.0%
AAATCAAGGTCACAAAGATT+AGG - chr2.4:13772339-13772358 MS.gene43039:CDS 30.0%
AATCAAGGTCACAAAGATTA+GGG - chr2.4:13772340-13772359 MS.gene43039:CDS 30.0%
ATTTACGTACATCAGCAATA+AGG + chr2.4:13772323-13772342 None:intergenic 30.0%
TGAAGGGATAATAATGAAGT+TGG + chr2.4:13772009-13772028 None:intergenic 30.0%
TTTACGTACATCAGCAATAA+GGG + chr2.4:13772322-13772341 None:intergenic 30.0%
! GCTTGTAATCATTTTGTTGA+AGG + chr2.4:13772026-13772045 None:intergenic 30.0%
! TCATCATAGCTTTTCGTATT+TGG + chr2.4:13772063-13772082 None:intergenic 30.0%
!! GATTGATGATGATGATGAAA+AGG - chr2.4:13772083-13772102 MS.gene43039:CDS 30.0%
AAGTTGGAATATGGCAAGTA+GGG + chr2.4:13771993-13772012 None:intergenic 35.0%
AGGAACAAAAGCTGAAAGAA+TGG - chr2.4:13772229-13772248 MS.gene43039:CDS 35.0%
GCATTAACAATCAATGCATG+AGG + chr2.4:13772291-13772310 None:intergenic 35.0%
GGAACAAAAGCTGAAAGAAT+GGG - chr2.4:13772230-13772249 MS.gene43039:CDS 35.0%
TAAAGAGAATGGTACTGCTA+GGG - chr2.4:13772122-13772141 MS.gene43039:CDS 35.0%
TGACTCATAACATCTCAACA+TGG - chr2.4:13771956-13771975 MS.gene43039:CDS 35.0%
TTGCTGATGTACGTAAATCA+AGG - chr2.4:13772325-13772344 MS.gene43039:CDS 35.0%
! AGGTTAAAGTACCTGAAAAC+TGG - chr2.4:13772202-13772221 MS.gene43039:CDS 35.0%
! GGTTAAAGTACCTGAAAACT+GGG - chr2.4:13772203-13772222 MS.gene43039:CDS 35.0%
CTCTTTACTCTCTTCCTCTT+TGG + chr2.4:13772109-13772128 None:intergenic 40.0%
GAAGTTGGAATATGGCAAGT+AGG + chr2.4:13771994-13772013 None:intergenic 40.0%
GAGGAAGAGAGTAAAGAGAA+TGG - chr2.4:13772111-13772130 MS.gene43039:CDS 40.0%
GTAAAGAGAATGGTACTGCT+AGG - chr2.4:13772121-13772140 MS.gene43039:CDS 40.0%
TGCTAGGGTAGAGGAAATTA+CGG - chr2.4:13772137-13772156 MS.gene43039:CDS 40.0%
! TGATGATGAAAAGGCCAAAG+AGG - chr2.4:13772092-13772111 MS.gene43039:CDS 40.0%
AGGCATAGGGAAGAGATGAA+GGG - chr2.4:13772177-13772196 MS.gene43039:CDS 45.0%
AGTACCTGAAAACTGGGAGA+AGG - chr2.4:13772209-13772228 MS.gene43039:CDS 45.0%
CTAGGGTAGAGGAAATTACG+GGG - chr2.4:13772139-13772158 MS.gene43039:CDS 45.0%
GCTAGGGTAGAGGAAATTAC+GGG - chr2.4:13772138-13772157 MS.gene43039:CDS 45.0%
TAGGGAAGAGATGAAGGGAA+AGG - chr2.4:13772182-13772201 MS.gene43039:CDS 45.0%
! TGAGAGGTTGAAGAGGCATA+GGG - chr2.4:13772164-13772183 MS.gene43039:CDS 45.0%
! TGTTCCTTCTCCCAGTTTTC+AGG + chr2.4:13772216-13772235 None:intergenic 45.0%
GAATGGTACTGCTAGGGTAG+AGG - chr2.4:13772128-13772147 MS.gene43039:CDS 50.0%
GAGGCATAGGGAAGAGATGA+AGG - chr2.4:13772176-13772195 MS.gene43039:CDS 50.0%
! GTGAGAGGTTGAAGAGGCAT+AGG - chr2.4:13772163-13772182 MS.gene43039:CDS 50.0%
AGGAAATTACGGGGCGTGAG+AGG - chr2.4:13772148-13772167 MS.gene43039:CDS 55.0%
CGGGGCGTGAGAGGTTGAAG+AGG - chr2.4:13772157-13772176 MS.gene43039:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 13771937 13772374 13771937 ID=MS.gene43039
chr2.4 mRNA 13771937 13772374 13771937 ID=MS.gene43039.t1;Parent=MS.gene43039
chr2.4 exon 13771937 13772374 13771937 ID=MS.gene43039.t1.exon1;Parent=MS.gene43039.t1
chr2.4 CDS 13771937 13772374 13771937 ID=cds.MS.gene43039.t1;Parent=MS.gene43039.t1
Gene Sequence

>MS.gene43039

ATGGATCAGGAAAATAGATTGACTCATAACATCTCAACATGGAAGAGAAGAAACCCTACTTGCCATATTCCAACTTCATTATTATCCCTTCAACAAAATGATTACAAGCTCGAGTGCAACTCTCCAAATACGAAAAGCTATGATGAGATTGATGATGATGATGAAAAGGCCAAAGAGGAAGAGAGTAAAGAGAATGGTACTGCTAGGGTAGAGGAAATTACGGGGCGTGAGAGGTTGAAGAGGCATAGGGAAGAGATGAAGGGAAAGGTTAAAGTACCTGAAAACTGGGAGAAGGAACAAAAGCTGAAAGAATGGGTTGACTATACTACGTTTGATGCATTGATATTTGCTCCTCATGCATTGATTGTTAATGCTCGTGATGCCCTTATTGCTGATGTACGTAAATCAAGGTCACAAAGATTAGGGATCCATACCTAG

Protein sequence

>MS.gene43039.t1

MDQENRLTHNISTWKRRNPTCHIPTSLLSLQQNDYKLECNSPNTKSYDEIDDDDEKAKEEESKENGTARVEEITGRERLKRHREEMKGKVKVPENWEKEQKLKEWVDYTTFDALIFAPHALIVNARDALIADVRKSRSQRLGIHT