Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45003.t1 | GAU40887.1 | 86.5 | 333 | 33 | 1 | 1 | 333 | 1 | 321 | 3.20E-164 | 587.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45003.t1 | E7C196 | 60.4 | 331 | 112 | 2 | 9 | 332 | 8 | 326 | 2.8e-113 | 409.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45003.t1 | A0A2Z6PC20 | 86.5 | 333 | 33 | 1 | 1 | 333 | 1 | 321 | 2.3e-164 | 587.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049356 | MS.gene45003 | 0.836437 | 9.36E-57 | -1.69E-46 |
| MS.gene049369 | MS.gene45003 | 0.823811 | 1.13E-53 | -1.69E-46 |
| MS.gene049396 | MS.gene45003 | 0.817746 | 2.81E-52 | -1.69E-46 |
| MS.gene050060 | MS.gene45003 | 0.8015 | 8.75E-49 | -1.69E-46 |
| MS.gene050670 | MS.gene45003 | 0.813474 | 2.51E-51 | -1.69E-46 |
| MS.gene05105 | MS.gene45003 | 0.800002 | 1.77E-48 | -1.69E-46 |
| MS.gene051525 | MS.gene45003 | 0.81072 | 1.00E-50 | -1.69E-46 |
| MS.gene051692 | MS.gene45003 | 0.807099 | 5.97E-50 | -1.69E-46 |
| MS.gene052134 | MS.gene45003 | 0.822869 | 1.88E-53 | -1.69E-46 |
| MS.gene052135 | MS.gene45003 | 0.832765 | 7.84E-56 | -1.69E-46 |
| MS.gene052136 | MS.gene45003 | 0.845142 | 4.88E-59 | -1.69E-46 |
| MS.gene052188 | MS.gene45003 | 0.800874 | 1.17E-48 | -1.69E-46 |
| MS.gene052253 | MS.gene45003 | 0.813531 | 2.44E-51 | -1.69E-46 |
| MS.gene052340 | MS.gene45003 | 0.801307 | 9.58E-49 | -1.69E-46 |
| MS.gene052346 | MS.gene45003 | 0.833774 | 4.40E-56 | -1.69E-46 |
| MS.gene052414 | MS.gene45003 | 0.883651 | 3.75E-71 | -1.69E-46 |
| MS.gene052481 | MS.gene45003 | 0.813174 | 2.92E-51 | -1.69E-46 |
| MS.gene052485 | MS.gene45003 | 0.8032 | 3.90E-49 | -1.69E-46 |
| MS.gene052982 | MS.gene45003 | 0.813288 | 2.76E-51 | -1.69E-46 |
| MS.gene052999 | MS.gene45003 | 0.81146 | 6.93E-51 | -1.69E-46 |
| MS.gene053449 | MS.gene45003 | 0.862822 | 3.86E-64 | -1.69E-46 |
| MS.gene053889 | MS.gene45003 | 0.8059 | 1.07E-49 | -1.69E-46 |
| MS.gene053912 | MS.gene45003 | 0.834921 | 2.27E-56 | -1.69E-46 |
| MS.gene054349 | MS.gene45003 | 0.816115 | 6.53E-52 | -1.69E-46 |
| MS.gene05494 | MS.gene45003 | 0.8029 | 4.50E-49 | -1.69E-46 |
| MS.gene055116 | MS.gene45003 | 0.825047 | 5.80E-54 | -1.69E-46 |
| MS.gene055741 | MS.gene45003 | 0.821718 | 3.48E-53 | -1.69E-46 |
| MS.gene055744 | MS.gene45003 | 0.828361 | 9.39E-55 | -1.69E-46 |
| MS.gene055909 | MS.gene45003 | 0.831706 | 1.43E-55 | -1.69E-46 |
| MS.gene055911 | MS.gene45003 | 0.829951 | 3.86E-55 | -1.69E-46 |
| MS.gene056422 | MS.gene45003 | 0.81705 | 4.03E-52 | -1.69E-46 |
| MS.gene056511 | MS.gene45003 | 0.829397 | 5.27E-55 | -1.69E-46 |
| MS.gene057019 | MS.gene45003 | 0.80045 | 1.43E-48 | -1.69E-46 |
| MS.gene057086 | MS.gene45003 | 0.82256 | 2.22E-53 | -1.69E-46 |
| MS.gene057124 | MS.gene45003 | 0.818379 | 2.02E-52 | -1.69E-46 |
| MS.gene057289 | MS.gene45003 | 0.819223 | 1.30E-52 | -1.69E-46 |
| MS.gene057396 | MS.gene45003 | 0.807875 | 4.08E-50 | -1.69E-46 |
| MS.gene057734 | MS.gene45003 | 0.837029 | 6.61E-57 | -1.69E-46 |
| MS.gene057749 | MS.gene45003 | 0.833725 | 4.52E-56 | -1.69E-46 |
| MS.gene057823 | MS.gene45003 | 0.835933 | 1.26E-56 | -1.69E-46 |
| MS.gene058120 | MS.gene45003 | 0.805414 | 1.35E-49 | -1.69E-46 |
| MS.gene058376 | MS.gene45003 | 0.806152 | 9.45E-50 | -1.69E-46 |
| MS.gene058444 | MS.gene45003 | 0.81254 | 4.03E-51 | -1.69E-46 |
| MS.gene058928 | MS.gene45003 | 0.841936 | 3.51E-58 | -1.69E-46 |
| MS.gene059011 | MS.gene45003 | 0.800287 | 1.55E-48 | -1.69E-46 |
| MS.gene059366 | MS.gene45003 | 0.813426 | 2.58E-51 | -1.69E-46 |
| MS.gene059402 | MS.gene45003 | -0.801344 | 9.41E-49 | -1.69E-46 |
| MS.gene059420 | MS.gene45003 | 0.844972 | 5.43E-59 | -1.69E-46 |
| MS.gene059455 | MS.gene45003 | 0.805329 | 1.41E-49 | -1.69E-46 |
| MS.gene059674 | MS.gene45003 | 0.830516 | 2.81E-55 | -1.69E-46 |
| MS.gene059807 | MS.gene45003 | 0.856208 | 3.76E-62 | -1.69E-46 |
| MS.gene060372 | MS.gene45003 | 0.831698 | 1.44E-55 | -1.69E-46 |
| MS.gene060403 | MS.gene45003 | 0.813878 | 2.05E-51 | -1.69E-46 |
| MS.gene060835 | MS.gene45003 | 0.80585 | 1.09E-49 | -1.69E-46 |
| MS.gene060837 | MS.gene45003 | 0.83014 | 3.47E-55 | -1.69E-46 |
| MS.gene06097 | MS.gene45003 | 0.809353 | 1.97E-50 | -1.69E-46 |
| MS.gene061154 | MS.gene45003 | 0.819191 | 1.32E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45003.t1 | MTR_4g072350 | 78.679 | 333 | 59 | 1 | 1 | 333 | 1 | 321 | 0.0 | 539 |
| MS.gene45003.t1 | MTR_4g072360 | 74.699 | 332 | 72 | 1 | 1 | 332 | 1 | 320 | 0.0 | 514 |
| MS.gene45003.t1 | MTR_4g072320 | 74.398 | 332 | 67 | 3 | 1 | 332 | 1 | 314 | 0.0 | 506 |
| MS.gene45003.t1 | MTR_6g073110 | 57.273 | 330 | 129 | 1 | 1 | 330 | 1 | 318 | 8.06e-143 | 406 |
| MS.gene45003.t1 | MTR_0374s0050 | 49.552 | 335 | 146 | 3 | 1 | 332 | 1 | 315 | 3.37e-109 | 321 |
| MS.gene45003.t1 | MTR_3g083130 | 48.071 | 337 | 144 | 8 | 1 | 330 | 1 | 313 | 3.10e-98 | 293 |
| MS.gene45003.t1 | MTR_5g097900 | 48.428 | 318 | 136 | 7 | 16 | 330 | 19 | 311 | 3.20e-89 | 270 |
| MS.gene45003.t1 | MTR_5g097910 | 50.000 | 296 | 129 | 5 | 16 | 309 | 19 | 297 | 5.76e-89 | 269 |
| MS.gene45003.t1 | MTR_5g097940 | 50.000 | 296 | 129 | 5 | 16 | 309 | 19 | 297 | 5.76e-89 | 269 |
| MS.gene45003.t1 | MTR_4g036845 | 43.636 | 330 | 167 | 6 | 5 | 332 | 2 | 314 | 8.49e-88 | 266 |
| MS.gene45003.t1 | MTR_8g070095 | 43.465 | 329 | 159 | 7 | 11 | 332 | 10 | 318 | 3.23e-84 | 257 |
| MS.gene45003.t1 | MTR_6g072650 | 81.333 | 150 | 6 | 1 | 184 | 333 | 10 | 137 | 9.58e-84 | 249 |
| MS.gene45003.t1 | MTR_8g070115 | 41.796 | 323 | 169 | 5 | 11 | 332 | 10 | 314 | 3.30e-77 | 239 |
| MS.gene45003.t1 | MTR_4g021350 | 41.265 | 332 | 172 | 7 | 3 | 332 | 2 | 312 | 2.21e-74 | 232 |
| MS.gene45003.t1 | MTR_7g063580 | 40.774 | 336 | 176 | 7 | 1 | 332 | 37 | 353 | 1.30e-72 | 229 |
| MS.gene45003.t1 | MTR_4g021410 | 40.187 | 321 | 162 | 7 | 11 | 330 | 11 | 302 | 4.39e-68 | 215 |
| MS.gene45003.t1 | MTR_4g021350 | 41.118 | 304 | 159 | 5 | 3 | 306 | 2 | 285 | 1.85e-66 | 211 |
| MS.gene45003.t1 | MTR_4g021450 | 39.339 | 333 | 172 | 7 | 1 | 332 | 1 | 304 | 3.99e-66 | 210 |
| MS.gene45003.t1 | MTR_7g070500 | 36.533 | 323 | 181 | 7 | 11 | 330 | 17 | 318 | 6.09e-64 | 205 |
| MS.gene45003.t1 | MTR_4g021410 | 42.215 | 289 | 140 | 6 | 43 | 330 | 3 | 265 | 7.04e-64 | 203 |
| MS.gene45003.t1 | MTR_7g070500 | 36.213 | 301 | 171 | 5 | 11 | 311 | 17 | 296 | 4.19e-58 | 189 |
| MS.gene45003.t1 | MTR_4g072060 | 36.364 | 308 | 168 | 7 | 9 | 306 | 3 | 292 | 2.33e-52 | 175 |
| MS.gene45003.t1 | MTR_4g021410 | 42.231 | 251 | 119 | 5 | 81 | 330 | 1 | 226 | 2.36e-52 | 172 |
| MS.gene45003.t1 | MTR_4g021450 | 41.502 | 253 | 122 | 5 | 81 | 332 | 1 | 228 | 1.17e-50 | 168 |
| MS.gene45003.t1 | MTR_4g021450 | 45.556 | 180 | 93 | 2 | 154 | 332 | 10 | 185 | 5.66e-49 | 162 |
| MS.gene45003.t1 | MTR_6g072710 | 62.832 | 113 | 42 | 0 | 202 | 314 | 1 | 113 | 4.91e-48 | 157 |
| MS.gene45003.t1 | MTR_6g072705 | 81.481 | 54 | 10 | 0 | 111 | 164 | 32 | 85 | 1.61e-25 | 98.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45003.t1 | AT1G59960 | 48.368 | 337 | 157 | 3 | 1 | 332 | 1 | 325 | 1.00e-115 | 338 |
| MS.gene45003.t1 | AT1G59950 | 48.265 | 317 | 151 | 2 | 17 | 332 | 15 | 319 | 3.51e-107 | 316 |
| MS.gene45003.t1 | AT2G37790 | 45.820 | 323 | 155 | 5 | 11 | 332 | 10 | 313 | 1.39e-91 | 276 |
| MS.gene45003.t1 | AT2G37770 | 45.820 | 323 | 156 | 5 | 11 | 332 | 10 | 314 | 3.10e-89 | 270 |
| MS.gene45003.t1 | AT3G53880 | 43.963 | 323 | 162 | 5 | 11 | 332 | 10 | 314 | 2.38e-85 | 260 |
| MS.gene45003.t1 | AT5G62420 | 41.088 | 331 | 163 | 7 | 11 | 332 | 8 | 315 | 1.71e-81 | 250 |
| MS.gene45003.t1 | AT2G37760 | 43.034 | 323 | 161 | 5 | 11 | 332 | 10 | 310 | 9.26e-81 | 248 |
| MS.gene45003.t1 | AT2G37760 | 43.034 | 323 | 161 | 5 | 11 | 332 | 10 | 310 | 9.26e-81 | 248 |
| MS.gene45003.t1 | AT3G53880 | 43.243 | 296 | 150 | 4 | 11 | 306 | 10 | 287 | 1.48e-74 | 233 |
| MS.gene45003.t1 | AT2G37760 | 42.953 | 298 | 148 | 4 | 11 | 308 | 10 | 285 | 3.85e-74 | 231 |
| MS.gene45003.t1 | AT5G01670 | 38.253 | 332 | 181 | 8 | 2 | 330 | 9 | 319 | 8.59e-74 | 231 |
| MS.gene45003.t1 | AT2G37760 | 42.475 | 299 | 150 | 4 | 11 | 309 | 10 | 286 | 9.88e-74 | 229 |
| MS.gene45003.t1 | AT2G37760 | 42.475 | 299 | 150 | 4 | 11 | 309 | 10 | 286 | 9.88e-74 | 229 |
| MS.gene45003.t1 | AT5G01670 | 35.376 | 359 | 181 | 9 | 2 | 330 | 9 | 346 | 8.51e-68 | 216 |
| MS.gene45003.t1 | AT2G37760 | 43.382 | 272 | 138 | 4 | 62 | 332 | 8 | 264 | 6.68e-67 | 211 |
| MS.gene45003.t1 | AT2G37760 | 43.382 | 272 | 138 | 4 | 62 | 332 | 8 | 264 | 6.68e-67 | 211 |
| MS.gene45003.t1 | AT2G21260 | 35.922 | 309 | 170 | 7 | 8 | 306 | 2 | 292 | 9.55e-60 | 194 |
| MS.gene45003.t1 | AT2G21250 | 34.951 | 309 | 173 | 7 | 8 | 306 | 2 | 292 | 1.62e-57 | 188 |
| MS.gene45003.t1 | AT2G37770 | 47.642 | 212 | 93 | 4 | 11 | 222 | 10 | 203 | 1.09e-55 | 183 |
| MS.gene45003.t1 | AT2G21250 | 35.200 | 250 | 134 | 7 | 8 | 247 | 2 | 233 | 3.36e-41 | 144 |
| MS.gene45003.t1 | AT2G21260 | 33.188 | 229 | 129 | 5 | 86 | 304 | 8 | 222 | 1.47e-36 | 132 |
| MS.gene45003.t1 | AT1G06690 | 25.084 | 299 | 158 | 10 | 42 | 304 | 94 | 362 | 1.96e-11 | 64.7 |
Find 74 sgRNAs with CRISPR-Local
Find 108 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGGAGCATACAAAGTATTT+TGG | 0.154726 | 6.3:-69143317 | MS.gene45003:CDS |
| TGAAGATCTCACTTCAATTT+TGG | 0.164020 | 6.3:-69144090 | MS.gene45003:CDS |
| GCGATTCCCTTTGACATAAA+AGG | 0.234369 | 6.3:-69143682 | MS.gene45003:CDS |
| TTTGATACAGCTTCTATCTA+TGG | 0.275978 | 6.3:-69144038 | MS.gene45003:CDS |
| TAGAGAATGATGCTTGCTTT+AGG | 0.303216 | 6.3:+69143874 | None:intergenic |
| GTTGCTAACACCAATAGATT+TGG | 0.348806 | 6.3:+69143618 | None:intergenic |
| CATTTCACCGGCAGTTAATC+AGG | 0.357915 | 6.3:-69143554 | MS.gene45003:intron |
| CCATGAGGTGATACTAAATT+CGG | 0.362220 | 6.3:-69144168 | MS.gene45003:CDS |
| GAATCTGAACCAAGAAGAAT+TGG | 0.366890 | 6.3:-69142723 | MS.gene45003:CDS |
| AGAGAATGATGCTTGCTTTA+GGG | 0.367456 | 6.3:+69143875 | None:intergenic |
| TCTATTGGTGTTAGCAACTT+TGG | 0.367727 | 6.3:-69143613 | MS.gene45003:CDS |
| ATGGAAGATTGTTATAGATT+AGG | 0.377351 | 6.3:-69143646 | MS.gene45003:CDS |
| GCAGTTCCTAGGCTTATTAC+TGG | 0.378277 | 6.3:+69144134 | None:intergenic |
| TTAGGCTTAGCCAAATCTAT+TGG | 0.410561 | 6.3:-69143628 | MS.gene45003:CDS |
| AAAATCTGGAAATATTTGAT+TGG | 0.413557 | 6.3:-69142745 | MS.gene45003:CDS |
| GAAGTGAGATCTTCATTTGA+TGG | 0.423682 | 6.3:+69144098 | None:intergenic |
| AGAGAGGATGAAACAAAATC+TGG | 0.435542 | 6.3:-69142759 | MS.gene45003:CDS |
| AGCATCCGTGCACCATAACT+TGG | 0.440733 | 6.3:+69143932 | None:intergenic |
| CTGATTAACTGCCGGTGAAA+TGG | 0.455212 | 6.3:+69143555 | None:intergenic |
| AAATGGACCTTACAAATCCT+TGG | 0.461855 | 6.3:-69142642 | MS.gene45003:CDS |
| AGCTTGGCCTAGAGGTTCTT+CGG | 0.465069 | 6.3:+69144013 | None:intergenic |
| TCCACAATGCAGACTTTACA+AGG | 0.465814 | 6.3:-69142684 | MS.gene45003:CDS |
| TGGATCTCTACTTGATTCAC+TGG | 0.472213 | 6.3:-69143756 | MS.gene45003:CDS |
| AGCTCTTCCAAGGATTTGTA+AGG | 0.476136 | 6.3:+69142635 | None:intergenic |
| TAGAAAGAAGAGTGTAGCTC+AGG | 0.477815 | 6.3:-69143244 | MS.gene45003:intron |
| AGGGAATTCTGCATGAAGAA+AGG | 0.479737 | 6.3:-69143366 | MS.gene45003:CDS |
| ACATCTTCTTTCAATCTCAC+TGG | 0.482395 | 6.3:+69143733 | None:intergenic |
| GAGCATTGAGAACAAGGTCT+TGG | 0.483674 | 6.3:+69143907 | None:intergenic |
| CATGAGGTGATACTAAATTC+GGG | 0.484916 | 6.3:-69144167 | MS.gene45003:CDS |
| GTTGAAGGCTTTAACTTCAC+AGG | 0.492916 | 6.3:-69143712 | MS.gene45003:CDS |
| GAATCTGAGGTTGGAGTATG+TGG | 0.495354 | 6.3:-69143776 | MS.gene45003:CDS |
| GGGAATTCTGCATGAAGAAA+GGG | 0.499626 | 6.3:-69143365 | MS.gene45003:CDS |
| TGTGTGTGCATGGTCACCAT+TGG | 0.502232 | 6.3:-69143337 | MS.gene45003:CDS |
| CCATGCAGATAGCACTAAGA+TGG | 0.502673 | 6.3:-69142826 | MS.gene45003:intron |
| AGATCTTCATTTGATGGAAG+CGG | 0.503539 | 6.3:+69144104 | None:intergenic |
| GCGGAGATGTAGCAGTTCCT+AGG | 0.506737 | 6.3:+69144123 | None:intergenic |
| GTGTGTGCATGGTCACCATT+GGG | 0.511118 | 6.3:-69143336 | MS.gene45003:CDS |
| TATTCTTGAGGAAATTGCTC+AGG | 0.512563 | 6.3:-69143268 | MS.gene45003:CDS |
| ATTTCTGTAGCCAATGTCAA+TGG | 0.520632 | 6.3:+69144061 | None:intergenic |
| CAAATCCTTGGAAGAGCTGT+GGG | 0.525762 | 6.3:-69142630 | MS.gene45003:CDS |
| TCTTATACCTGATTAACTGC+CGG | 0.530912 | 6.3:+69143547 | None:intergenic |
| CCTTTGACATAAAAGGAACA+TGG | 0.535164 | 6.3:-69143675 | MS.gene45003:CDS |
| TACTTCCAAGTTATGGTGCA+CGG | 0.539944 | 6.3:-69143937 | MS.gene45003:CDS |
| AGATTGAAAGAAGATGTTGA+AGG | 0.547100 | 6.3:-69143727 | MS.gene45003:CDS |
| CATGGCAGCAAGGAAAACTC+AGG | 0.551755 | 6.3:-69143386 | MS.gene45003:CDS |
| TTGGGGTTCTAATGCTGTGA+TGG | 0.554067 | 6.3:-69143298 | MS.gene45003:CDS |
| GGAAAATGCAACCATTTCAC+CGG | 0.554214 | 6.3:-69143566 | MS.gene45003:CDS |
| GTGTCAAAAGCTTTAGAGCT+CGG | 0.554884 | 6.3:-69143990 | MS.gene45003:CDS |
| CCGAATTTAGTATCACCTCA+TGG | 0.575461 | 6.3:+69144168 | None:intergenic |
| TAATCTATAACAATCTTCCA+TGG | 0.576595 | 6.3:+69143648 | None:intergenic |
| TTGGTTGATGCCATTGACAT+TGG | 0.577375 | 6.3:-69144071 | MS.gene45003:CDS |
| GAATGATGCTTGCTTTAGGG+TGG | 0.578769 | 6.3:+69143878 | None:intergenic |
| ACCATCCCACAGCTCTTCCA+AGG | 0.580081 | 6.3:+69142625 | None:intergenic |
| AGGCTTTAACTTCACAGGCA+AGG | 0.580507 | 6.3:-69143707 | MS.gene45003:CDS |
| AAAAGGAACATGGAAAGCCA+TGG | 0.591101 | 6.3:-69143665 | MS.gene45003:CDS |
| ATGGTGACCATGCACACACA+TGG | 0.592094 | 6.3:+69143340 | None:intergenic |
| ACAAATCCTTGGAAGAGCTG+TGG | 0.595019 | 6.3:-69142631 | MS.gene45003:CDS |
| TGAAAAGCTTCAACAAAGAG+AGG | 0.595864 | 6.3:-69142775 | MS.gene45003:CDS |
| TTTGACACAGCTTGGCCTAG+AGG | 0.596039 | 6.3:+69144005 | None:intergenic |
| AAGATGCCAGTAATAAGCCT+AGG | 0.596510 | 6.3:-69144140 | MS.gene45003:CDS |
| TATGGTACCGAAGAACCTCT+AGG | 0.597666 | 6.3:-69144020 | MS.gene45003:CDS |
| AGGTAGAAATGAATCCATCA+TGG | 0.603843 | 6.3:-69143404 | MS.gene45003:CDS |
| ATGAATCCATCATGGCAGCA+AGG | 0.616292 | 6.3:-69143396 | MS.gene45003:CDS |
| AATACTTTGTATGCTCCCAA+TGG | 0.618795 | 6.3:+69143321 | None:intergenic |
| AAGGGATCCATGTGTGTGCA+TGG | 0.626859 | 6.3:-69143347 | MS.gene45003:CDS |
| GCCTTGTAAAGTCTGCATTG+TGG | 0.641275 | 6.3:+69142683 | None:intergenic |
| GATGGAGAATGCTATTCTTG+AGG | 0.641402 | 6.3:-69143280 | MS.gene45003:CDS |
| CTAAGATGGATACATGAGCA+AGG | 0.646695 | 6.3:-69142812 | MS.gene45003:CDS |
| ATGGCAGCAAGGAAAACTCA+GGG | 0.651861 | 6.3:-69143385 | MS.gene45003:CDS |
| ATGAGGTGATACTAAATTCG+GGG | 0.654424 | 6.3:-69144166 | MS.gene45003:CDS |
| TAAGATGGATACATGAGCAA+GGG | 0.664827 | 6.3:-69142811 | MS.gene45003:CDS |
| TCCTTGGAAGAGCTGTGGGA+TGG | 0.668107 | 6.3:-69142626 | MS.gene45003:CDS |
| TGAGGTGATACTAAATTCGG+GGG | 0.676837 | 6.3:-69144165 | MS.gene45003:CDS |
| GGAAGCAACTAAACTCCATG+AGG | 0.702832 | 6.3:-69144183 | MS.gene45003:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTTGTTGGAATTTTTT+AGG | - | chr6.3:69143003-69143022 | MS.gene45003:intron | 15.0% |
| !! | AAAATCTGGAAATATTTGAT+TGG | - | chr6.3:69144056-69144075 | MS.gene45003:CDS | 20.0% |
| !! | AATACAAACTGAAAAAAAAG+AGG | + | chr6.3:69142954-69142973 | None:intergenic | 20.0% |
| !! | AGTATTAACGTAATATTGAT+TGG | + | chr6.3:69143322-69143341 | None:intergenic | 20.0% |
| !! | ATACAAACTGAAAAAAAAGA+GGG | + | chr6.3:69142953-69142972 | None:intergenic | 20.0% |
| !!! | GAAATGTTTTTATCTGAAAA+TGG | - | chr6.3:69144142-69144161 | MS.gene45003:CDS | 20.0% |
| ! | AAGCTTAAAATGCTAGATAA+AGG | - | chr6.3:69143654-69143673 | MS.gene45003:CDS | 25.0% |
| ! | AGCTTAAAATGCTAGATAAA+GGG | - | chr6.3:69143655-69143674 | MS.gene45003:CDS | 25.0% |
| ! | ATGGAAGATTGTTATAGATT+AGG | - | chr6.3:69143155-69143174 | MS.gene45003:intron | 25.0% |
| ! | TAATCTATAACAATCTTCCA+TGG | + | chr6.3:69143156-69143175 | None:intergenic | 25.0% |
| !! | TTTTCATTACTTCCAAGTTA+TGG | - | chr6.3:69142857-69142876 | MS.gene45003:intron | 25.0% |
| AGATTGAAAGAAGATGTTGA+AGG | - | chr6.3:69143074-69143093 | MS.gene45003:intron | 30.0% | |
| TAATGACTCGTACAAATACT+CGG | + | chr6.3:69143298-69143317 | None:intergenic | 30.0% | |
| TGTTATGAAATCCACTCAAT+TGG | - | chr6.3:69143351-69143370 | MS.gene45003:CDS | 30.0% | |
| TTTGATACAGCTTCTATCTA+TGG | - | chr6.3:69142763-69142782 | MS.gene45003:CDS | 30.0% | |
| ! | CATGTTCCTTTTATGTCAAA+GGG | + | chr6.3:69143128-69143147 | None:intergenic | 30.0% |
| ! | GCTTATACATAAGCACTTTT+AGG | - | chr6.3:69143877-69143896 | MS.gene45003:CDS | 30.0% |
| ! | TGAAGATCTCACTTCAATTT+TGG | - | chr6.3:69142711-69142730 | MS.gene45003:CDS | 30.0% |
| ! | TGATTTTGTCCAATTCTTCT+TGG | + | chr6.3:69144090-69144109 | None:intergenic | 30.0% |
| !!! | ATTTTTTAGGAATCTGAGGT+TGG | - | chr6.3:69143016-69143035 | MS.gene45003:intron | 30.0% |
| !!! | TGGAATTTTTTAGGAATCTG+AGG | - | chr6.3:69143012-69143031 | MS.gene45003:intron | 30.0% |
| AAATGGACCTTACAAATCCT+TGG | - | chr6.3:69144159-69144178 | MS.gene45003:CDS | 35.0% | |
| AATACTTTGTATGCTCCCAA+TGG | + | chr6.3:69143483-69143502 | None:intergenic | 35.0% | |
| ACATCTTCTTTCAATCTCAC+TGG | + | chr6.3:69143071-69143090 | None:intergenic | 35.0% | |
| AGAGAATGATGCTTGCTTTA+GGG | + | chr6.3:69142929-69142948 | None:intergenic | 35.0% | |
| AGAGAGGATGAAACAAAATC+TGG | - | chr6.3:69144042-69144061 | MS.gene45003:CDS | 35.0% | |
| AGATCTTCATTTGATGGAAG+CGG | + | chr6.3:69142700-69142719 | None:intergenic | 35.0% | |
| AGGTAGAAATGAATCCATCA+TGG | - | chr6.3:69143397-69143416 | MS.gene45003:CDS | 35.0% | |
| ATGAGGTGATACTAAATTCG+GGG | - | chr6.3:69142635-69142654 | MS.gene45003:CDS | 35.0% | |
| ATTTCTGTAGCCAATGTCAA+TGG | + | chr6.3:69142743-69142762 | None:intergenic | 35.0% | |
| CATGAGGTGATACTAAATTC+GGG | - | chr6.3:69142634-69142653 | MS.gene45003:CDS | 35.0% | |
| CCATGAGGTGATACTAAATT+CGG | - | chr6.3:69142633-69142652 | MS.gene45003:CDS | 35.0% | |
| CCTTTGACATAAAAGGAACA+TGG | - | chr6.3:69143126-69143145 | MS.gene45003:intron | 35.0% | |
| GAAGTGAGATCTTCATTTGA+TGG | + | chr6.3:69142706-69142725 | None:intergenic | 35.0% | |
| GAATCTGAACCAAGAAGAAT+TGG | - | chr6.3:69144078-69144097 | MS.gene45003:CDS | 35.0% | |
| GTCAGATTACAAGAGTTCAT+TGG | - | chr6.3:69143728-69143747 | MS.gene45003:CDS | 35.0% | |
| GTTGCTAACACCAATAGATT+TGG | + | chr6.3:69143186-69143205 | None:intergenic | 35.0% | |
| TAACTATCGTGAAGAGCTTA+TGG | + | chr6.3:69143795-69143814 | None:intergenic | 35.0% | |
| TAAGATGGATACATGAGCAA+GGG | - | chr6.3:69143990-69144009 | MS.gene45003:CDS | 35.0% | |
| TAGAGAATGATGCTTGCTTT+AGG | + | chr6.3:69142930-69142949 | None:intergenic | 35.0% | |
| TATTCTTGAGGAAATTGCTC+AGG | - | chr6.3:69143533-69143552 | MS.gene45003:intron | 35.0% | |
| TCTTATACCTGATTAACTGC+CGG | + | chr6.3:69143257-69143276 | None:intergenic | 35.0% | |
| TGAAAAGCTTCAACAAAGAG+AGG | - | chr6.3:69144026-69144045 | MS.gene45003:CDS | 35.0% | |
| TTAGGCTTAGCCAAATCTAT+TGG | - | chr6.3:69143173-69143192 | MS.gene45003:intron | 35.0% | |
| ! | CCATGTTCCTTTTATGTCAA+AGG | + | chr6.3:69143129-69143148 | None:intergenic | 35.0% |
| !! | GGAGCATACAAAGTATTTTG+GGG | - | chr6.3:69143486-69143505 | MS.gene45003:intron | 35.0% |
| !! | GGGAGCATACAAAGTATTTT+GGG | - | chr6.3:69143485-69143504 | MS.gene45003:intron | 35.0% |
| !! | TCTATTGGTGTTAGCAACTT+TGG | - | chr6.3:69143188-69143207 | MS.gene45003:intron | 35.0% |
| !! | TGGGAGCATACAAAGTATTT+TGG | - | chr6.3:69143484-69143503 | MS.gene45003:intron | 35.0% |
| !!! | TTTTGAGAGCATTGAGAACA+AGG | + | chr6.3:69142903-69142922 | None:intergenic | 35.0% |
| AAAAGGAACATGGAAAGCCA+TGG | - | chr6.3:69143136-69143155 | MS.gene45003:intron | 40.0% | |
| AAGATGCCAGTAATAAGCCT+AGG | - | chr6.3:69142661-69142680 | MS.gene45003:CDS | 40.0% | |
| AGCTCTTCCAAGGATTTGTA+AGG | + | chr6.3:69144169-69144188 | None:intergenic | 40.0% | |
| AGGGAATTCTGCATGAAGAA+AGG | - | chr6.3:69143435-69143454 | MS.gene45003:intron | 40.0% | |
| CAAAAAGCTTTCCCTACTCT+TGG | - | chr6.3:69143214-69143233 | MS.gene45003:intron | 40.0% | |
| CCGAATTTAGTATCACCTCA+TGG | + | chr6.3:69142636-69142655 | None:intergenic | 40.0% | |
| CTAAGATGGATACATGAGCA+AGG | - | chr6.3:69143989-69144008 | MS.gene45003:CDS | 40.0% | |
| GATGGAGAATGCTATTCTTG+AGG | - | chr6.3:69143521-69143540 | MS.gene45003:intron | 40.0% | |
| GCGATTCCCTTTGACATAAA+AGG | - | chr6.3:69143119-69143138 | MS.gene45003:intron | 40.0% | |
| GGAAAATGCAACCATTTCAC+CGG | - | chr6.3:69143235-69143254 | MS.gene45003:intron | 40.0% | |
| GGGAATTCTGCATGAAGAAA+GGG | - | chr6.3:69143436-69143455 | MS.gene45003:intron | 40.0% | |
| TACTTCCAAGTTATGGTGCA+CGG | - | chr6.3:69142864-69142883 | MS.gene45003:intron | 40.0% | |
| TCCACAATGCAGACTTTACA+AGG | - | chr6.3:69144117-69144136 | MS.gene45003:CDS | 40.0% | |
| TGAGGTGATACTAAATTCGG+GGG | - | chr6.3:69142636-69142655 | MS.gene45003:CDS | 40.0% | |
| TGCTAGATAAAGGGTCTGTT+TGG | - | chr6.3:69143664-69143683 | MS.gene45003:CDS | 40.0% | |
| TGGATCTCTACTTGATTCAC+TGG | - | chr6.3:69143045-69143064 | MS.gene45003:intron | 40.0% | |
| ! | CACGTCGATTATCTCGATTT+AGG | - | chr6.3:69143377-69143396 | MS.gene45003:CDS | 40.0% |
| ! | CTAAAGCTTTTGACACAGCT+TGG | + | chr6.3:69142807-69142826 | None:intergenic | 40.0% |
| ! | GGTTGCATTTTCCAAGAGTA+GGG | + | chr6.3:69143228-69143247 | None:intergenic | 40.0% |
| ! | GTTGAAGGCTTTAACTTCAC+AGG | - | chr6.3:69143089-69143108 | MS.gene45003:intron | 40.0% |
| ! | TAGAAAGAAGAGTGTAGCTC+AGG | - | chr6.3:69143557-69143576 | MS.gene45003:CDS | 40.0% |
| ! | TGGTTGCATTTTCCAAGAGT+AGG | + | chr6.3:69143229-69143248 | None:intergenic | 40.0% |
| ! | TTGGTTGATGCCATTGACAT+TGG | - | chr6.3:69142730-69142749 | MS.gene45003:CDS | 40.0% |
| !! | GTGTCAAAAGCTTTAGAGCT+CGG | - | chr6.3:69142811-69142830 | MS.gene45003:CDS | 40.0% |
| ACAAATCCTTGGAAGAGCTG+TGG | - | chr6.3:69144170-69144189 | MS.gene45003:CDS | 45.0% | |
| ATGAATCCATCATGGCAGCA+AGG | - | chr6.3:69143405-69143424 | MS.gene45003:CDS | 45.0% | |
| ATGGCAGCAAGGAAAACTCA+GGG | - | chr6.3:69143416-69143435 | MS.gene45003:intron | 45.0% | |
| CAAATCCTTGGAAGAGCTGT+GGG | - | chr6.3:69144171-69144190 | MS.gene45003:CDS | 45.0% | |
| CATTTCACCGGCAGTTAATC+AGG | - | chr6.3:69143247-69143266 | MS.gene45003:CDS | 45.0% | |
| CCATCTTAGTGCTATCTGCA+TGG | + | chr6.3:69143978-69143997 | None:intergenic | 45.0% | |
| CCATGCAGATAGCACTAAGA+TGG | - | chr6.3:69143975-69143994 | MS.gene45003:CDS | 45.0% | |
| CTGATTAACTGCCGGTGAAA+TGG | + | chr6.3:69143249-69143268 | None:intergenic | 45.0% | |
| GAGTTCATTGGAACAGCTGA+TGG | - | chr6.3:69143740-69143759 | MS.gene45003:CDS | 45.0% | |
| GCAGTTCCTAGGCTTATTAC+TGG | + | chr6.3:69142670-69142689 | None:intergenic | 45.0% | |
| GCCTTGTAAAGTCTGCATTG+TGG | + | chr6.3:69144121-69144140 | None:intergenic | 45.0% | |
| GGAAGCAACTAAACTCCATG+AGG | - | chr6.3:69142618-69142637 | MS.gene45003:CDS | 45.0% | |
| ! | AAAGAAGAGTGTAGCTCAGG+TGG | - | chr6.3:69143560-69143579 | MS.gene45003:CDS | 45.0% |
| ! | AGGCTTTAACTTCACAGGCA+AGG | - | chr6.3:69143094-69143113 | MS.gene45003:intron | 45.0% |
| ! | AGTTTTCCTTGCTGCCATGA+TGG | + | chr6.3:69143414-69143433 | None:intergenic | 45.0% |
| ! | GAATCTGAGGTTGGAGTATG+TGG | - | chr6.3:69143025-69143044 | MS.gene45003:intron | 45.0% |
| ! | TATGGTACCGAAGAACCTCT+AGG | - | chr6.3:69142781-69142800 | MS.gene45003:CDS | 45.0% |
| ! | TTGGGGTTCTAATGCTGTGA+TGG | - | chr6.3:69143503-69143522 | MS.gene45003:intron | 45.0% |
| !! | GAATGATGCTTGCTTTAGGG+TGG | + | chr6.3:69142926-69142945 | None:intergenic | 45.0% |
| !! | GAGCATTGAGAACAAGGTCT+TGG | + | chr6.3:69142897-69142916 | None:intergenic | 45.0% |
| !!! | TATTTTTTTATATTTTTTGT+TGG | - | chr6.3:69142992-69143011 | MS.gene45003:intron | 5.0% |
| AAGGGATCCATGTGTGTGCA+TGG | - | chr6.3:69143454-69143473 | MS.gene45003:intron | 50.0% | |
| AGCATCCGTGCACCATAACT+TGG | + | chr6.3:69142872-69142891 | None:intergenic | 50.0% | |
| AGCTTGGCCTAGAGGTTCTT+CGG | + | chr6.3:69142791-69142810 | None:intergenic | 50.0% | |
| ATGGTGACCATGCACACACA+TGG | + | chr6.3:69143464-69143483 | None:intergenic | 50.0% | |
| CATGGCAGCAAGGAAAACTC+AGG | - | chr6.3:69143415-69143434 | MS.gene45003:intron | 50.0% | |
| CATTGGAACAGCTGATGGCA+TGG | - | chr6.3:69143745-69143764 | MS.gene45003:CDS | 50.0% | |
| GTGTGTGCATGGTCACCATT+GGG | - | chr6.3:69143465-69143484 | MS.gene45003:intron | 50.0% | |
| TCGACGTGCAACCAATTGAG+TGG | + | chr6.3:69143365-69143384 | None:intergenic | 50.0% | |
| TGTGTGTGCATGGTCACCAT+TGG | - | chr6.3:69143464-69143483 | MS.gene45003:intron | 50.0% | |
| TTTGACACAGCTTGGCCTAG+AGG | + | chr6.3:69142799-69142818 | None:intergenic | 50.0% | |
| ACCATCCCACAGCTCTTCCA+AGG | + | chr6.3:69144179-69144198 | None:intergenic | 55.0% | |
| GCGGAGATGTAGCAGTTCCT+AGG | + | chr6.3:69142681-69142700 | None:intergenic | 55.0% | |
| TCCTTGGAAGAGCTGTGGGA+TGG | - | chr6.3:69144175-69144194 | MS.gene45003:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 69142616 | 69144207 | 69142616 | ID=MS.gene45003 |
| chr6.3 | mRNA | 69142616 | 69144207 | 69142616 | ID=MS.gene45003.t1;Parent=MS.gene45003 |
| chr6.3 | exon | 69143855 | 69144207 | 69143855 | ID=MS.gene45003.t1.exon1;Parent=MS.gene45003.t1 |
| chr6.3 | CDS | 69143855 | 69144207 | 69143855 | ID=cds.MS.gene45003.t1;Parent=MS.gene45003.t1 |
| chr6.3 | exon | 69143555 | 69143798 | 69143555 | ID=MS.gene45003.t1.exon2;Parent=MS.gene45003.t1 |
| chr6.3 | CDS | 69143555 | 69143798 | 69143555 | ID=cds.MS.gene45003.t1;Parent=MS.gene45003.t1 |
| chr6.3 | exon | 69143245 | 69143424 | 69143245 | ID=MS.gene45003.t1.exon3;Parent=MS.gene45003.t1 |
| chr6.3 | CDS | 69143245 | 69143424 | 69143245 | ID=cds.MS.gene45003.t1;Parent=MS.gene45003.t1 |
| chr6.3 | exon | 69142616 | 69142840 | 69142616 | ID=MS.gene45003.t1.exon4;Parent=MS.gene45003.t1 |
| chr6.3 | CDS | 69142616 | 69142840 | 69142616 | ID=cds.MS.gene45003.t1;Parent=MS.gene45003.t1 |
| Gene Sequence |
| Protein sequence |
