AlfalfaGEDB | MS.gene45374

M. sativa cultivar XinJiangDaYe / MS.gene45374

KEGG: -
GO: -
PFAM: PF16884.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45374.t1	KEH18023.1	96.1	76	3	0	1	76	1	76	2.90E-33	150.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45374.t1	Q9SLN8	72.4	58	15	1	3	60	4	60	5.6e-17	87.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45374.t1	B7FL02	96.1	76	3	0	1	76	1	76	2.1e-33	150.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049283	MS.gene45374	0.810061	1.39E-50	-1.69E-46
MS.gene04984	MS.gene45374	0.802745	4.85E-49	-1.69E-46
MS.gene04991	MS.gene45374	-0.806912	6.53E-50	-1.69E-46
MS.gene050085	MS.gene45374	0.812428	4.26E-51	-1.69E-46
MS.gene050114	MS.gene45374	0.806995	6.27E-50	-1.69E-46
MS.gene050359	MS.gene45374	0.8009	1.16E-48	-1.69E-46
MS.gene050654	MS.gene45374	0.819497	1.13E-52	-1.69E-46
MS.gene051003	MS.gene45374	0.810057	1.39E-50	-1.69E-46
MS.gene051184	MS.gene45374	0.812906	3.35E-51	-1.69E-46
MS.gene051343	MS.gene45374	0.802826	4.66E-49	-1.69E-46
MS.gene051452	MS.gene45374	0.817193	3.74E-52	-1.69E-46
MS.gene051655	MS.gene45374	0.818332	2.07E-52	-1.69E-46
MS.gene051831	MS.gene45374	0.836498	9.03E-57	-1.69E-46
MS.gene051975	MS.gene45374	0.823574	1.29E-53	-1.69E-46
MS.gene051987	MS.gene45374	0.802674	5.02E-49	-1.69E-46
MS.gene052242	MS.gene45374	-0.82026	7.54E-53	-1.69E-46
MS.gene052434	MS.gene45374	0.800986	1.11E-48	-1.69E-46
MS.gene053058	MS.gene45374	0.83332	5.71E-56	-1.69E-46
MS.gene053295	MS.gene45374	0.832113	1.14E-55	-1.69E-46
MS.gene053564	MS.gene45374	0.8292	5.88E-55	-1.69E-46
MS.gene055311	MS.gene45374	0.808728	2.69E-50	-1.69E-46
MS.gene055441	MS.gene45374	0.801585	8.40E-49	-1.69E-46
MS.gene055442	MS.gene45374	0.812688	3.74E-51	-1.69E-46
MS.gene056181	MS.gene45374	0.800774	1.23E-48	-1.69E-46
MS.gene056605	MS.gene45374	0.800079	1.71E-48	-1.69E-46
MS.gene056638	MS.gene45374	0.800746	1.25E-48	-1.69E-46
MS.gene056787	MS.gene45374	0.847829	9.02E-60	-1.69E-46
MS.gene056849	MS.gene45374	0.846524	2.06E-59	-1.69E-46
MS.gene056975	MS.gene45374	0.81026	1.26E-50	-1.69E-46
MS.gene056986	MS.gene45374	0.812452	4.21E-51	-1.69E-46
MS.gene057999	MS.gene45374	0.81793	2.55E-52	-1.69E-46
MS.gene058054	MS.gene45374	0.807786	4.26E-50	-1.69E-46
MS.gene058708	MS.gene45374	-0.818612	1.79E-52	-1.69E-46
MS.gene059007	MS.gene45374	0.80136	9.34E-49	-1.69E-46
MS.gene059360	MS.gene45374	0.825283	5.10E-54	-1.69E-46
MS.gene06010	MS.gene45374	0.842797	2.08E-58	-1.69E-46
MS.gene06011	MS.gene45374	0.820739	5.85E-53	-1.69E-46
MS.gene060840	MS.gene45374	0.808836	2.55E-50	-1.69E-46
MS.gene060869	MS.gene45374	0.836613	8.45E-57	-1.69E-46
MS.gene060870	MS.gene45374	0.833657	4.70E-56	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45374.t1	MTR_8g010000	96.053	76	3	0	1	76	1	76	7.21e-48	154
MS.gene45374.t1	MTR_8g010030	73.684	76	20	0	1	76	1	76	1.18e-35	123
MS.gene45374.t1	MTR_8g009980	72.727	77	19	2	1	76	1	76	1.76e-33	117
MS.gene45374.t1	MTR_8g009840	74.026	77	17	2	1	76	1	75	3.45e-31	113
MS.gene45374.t1	MTR_8g009830	80.328	61	12	0	1	61	1	61	3.43e-30	108
MS.gene45374.t1	MTR_8g007325	75.410	61	15	0	1	61	1	61	5.27e-30	105
MS.gene45374.t1	MTR_8g009820	75.410	61	15	0	1	61	60	120	1.49e-28	105
MS.gene45374.t1	MTR_8g010020	90.909	44	4	0	1	44	1	44	6.29e-22	86.3
MS.gene45374.t1	MTR_4g082970	54.054	74	32	2	4	76	10	82	5.34e-15	67.8
MS.gene45374.t1	MTR_7g406860	44.444	72	34	2	1	69	3	71	1.04e-12	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45374.t1	AT3G03080	64.474	76	22	3	2	74	7	80	1.42e-23	91.3
MS.gene45374.t1	AT5G17000	60.563	71	25	2	6	74	5	74	1.97e-22	86.7
MS.gene45374.t1	AT5G16970	59.155	71	26	2	6	74	5	74	3.62e-22	87.4
MS.gene45374.t1	AT5G17000	60.563	71	25	2	6	74	5	74	7.21e-22	86.7
MS.gene45374.t1	AT5G16990	60.000	70	26	2	6	74	4	72	1.02e-21	86.3
MS.gene45374.t1	AT1G26320	65.000	60	20	1	4	63	9	67	2.05e-20	82.8
MS.gene45374.t1	AT1G26320	65.000	60	20	1	4	63	9	67	2.49e-20	82.4
MS.gene45374.t1	AT5G37940	62.295	61	22	1	6	66	12	71	7.36e-19	78.6
MS.gene45374.t1	AT5G38000	60.656	61	23	1	6	66	12	71	1.21e-18	77.8
MS.gene45374.t1	AT5G38000	60.656	61	23	1	6	66	12	71	1.36e-18	77.8
MS.gene45374.t1	AT5G38000	60.656	61	23	1	6	66	12	71	1.38e-18	77.8
MS.gene45374.t1	AT5G37980	60.656	61	23	1	6	66	12	71	2.00e-18	77.4
MS.gene45374.t1	AT1G65560	54.167	72	30	3	4	74	7	76	8.71e-17	72.8
MS.gene45374.t1	AT5G16960	52.632	76	33	2	1	74	1	75	8.99e-17	72.8
MS.gene45374.t1	AT5G16960	52.632	76	33	2	1	74	1	75	8.99e-17	72.8
MS.gene45374.t1	AT3G59845	56.923	65	25	2	4	66	5	68	2.20e-16	71.6

Find 14 sgRNAs with CRISPR-Local

Find 13 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGTGGATTGATAGAAGCTTT+TGG	0.308109	8.3:+78456047	None:intergenic
TTTAACCAAAACAACATCAT+TGG	0.334344	8.3:+78456118	None:intergenic
ACAACATCATTGGAACCTTC+TGG	0.339886	8.3:+78456128	None:intergenic
ATGTTGAGGTCTGATTCTTT+AGG	0.350907	8.3:+78456179	None:intergenic
AGGTTCCAATGATGTTGTTT+TGG	0.355569	8.3:-78456123	MS.gene45374:CDS
CTTCTGAAACATTATGTGTC+TGG	0.371901	8.3:-78456206	MS.gene45374:CDS
AGCCATAAACTTACAGATTG+AGG	0.442715	8.3:+78456014	None:intergenic
CTCATAAGAGTCCTCATGTA+AGG	0.474115	8.3:+78456074	None:intergenic
TTCTGGAATCTTAAGAGTAA+TGG	0.534462	8.3:+78456145	None:intergenic
ACTTACAGATTGAGGAACAT+AGG	0.548541	8.3:+78456022	None:intergenic
ATTACTCTTAAGATTCCAGA+AGG	0.569583	8.3:-78456143	MS.gene45374:CDS
TGGTACTGTCAACAATGTTG+AGG	0.627893	8.3:+78456165	None:intergenic
TGTCTTGTGATCCTTACATG+AGG	0.664048	8.3:-78456085	MS.gene45374:CDS
GATTGAGGAACATAGGCACG+TGG	0.744035	8.3:+78456029	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTTAACCAAAACAACATCAT+TGG	+	chr8.3:78456146-78456165	None:intergenic	25.0%
	ATTACTCTTAAGATTCCAGA+AGG	-	chr8.3:78456118-78456137	MS.gene45374:CDS	30.0%
!!	TTCTGGAATCTTAAGAGTAA+TGG	+	chr8.3:78456119-78456138	None:intergenic	30.0%
	ATGTTGAGGTCTGATTCTTT+AGG	+	chr8.3:78456085-78456104	None:intergenic	35.0%
!!	AGGTTCCAATGATGTTGTTT+TGG	-	chr8.3:78456138-78456157	MS.gene45374:CDS	35.0%
!!	CTTCTGAAACATTATGTGTC+TGG	-	chr8.3:78456055-78456074	MS.gene45374:CDS	35.0%
!!	GTGGATTGATAGAAGCTTTT+GGG	+	chr8.3:78456216-78456235	None:intergenic	35.0%
	ACAACATCATTGGAACCTTC+TGG	+	chr8.3:78456136-78456155	None:intergenic	40.0%
	CTCATAAGAGTCCTCATGTA+AGG	+	chr8.3:78456190-78456209	None:intergenic	40.0%
	TGGTACTGTCAACAATGTTG+AGG	+	chr8.3:78456099-78456118	None:intergenic	40.0%
	TGTCTTGTGATCCTTACATG+AGG	-	chr8.3:78456176-78456195	MS.gene45374:CDS	40.0%
!!	CGTGGATTGATAGAAGCTTT+TGG	+	chr8.3:78456217-78456236	None:intergenic	40.0%
	GATTGAGGAACATAGGCACG+TGG	+	chr8.3:78456235-78456254	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	78456028	78456255	78456028	ID=MS.gene45374
chr8.3	mRNA	78456028	78456255	78456028	ID=MS.gene45374.t1;Parent=MS.gene45374
chr8.3	exon	78456028	78456255	78456028	ID=MS.gene45374.t1.exon1;Parent=MS.gene45374.t1
chr8.3	CDS	78456028	78456255	78456028	ID=cds.MS.gene45374.t1;Parent=MS.gene45374.t1

Gene Sequence	>MS.gene45374 ATGGCACAAGTAAAGAACAAACAAGTTCTTCTGAAACATTATGTGTCTGGTTTTCCTAAAGAATCAGACCTCAACATTGTTGACAGTACCATTACTCTTAAGATTCCAGAAGGTTCCAATGATGTTGTTTTGGTTAAAAATCTTTACTTGTCTTGTGATCCTTACATGAGGACTCTTATGAGTGACCCAAAAGCTTCTATCAATCCACGTGCCTATGTTCCTCAATCT
Protein sequence	>MS.gene45374.t1 MAQVKNKQVLLKHYVSGFPKESDLNIVDSTITLKIPEGSNDVVLVKNLYLSCDPYMRTLMSDPKASINPRAYVPQS