Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50217.t1 | XP_024639719.1 | 88.3 | 180 | 18 | 1 | 1 | 177 | 1 | 180 | 3.60E-82 | 314.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50217.t1 | B7FH76 | 88.3 | 180 | 18 | 1 | 1 | 177 | 1 | 180 | 2.6e-82 | 314.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049297 | MS.gene50217 | 0.835182 | 1.95E-56 | -1.69E-46 |
| MS.gene049346 | MS.gene50217 | 0.809446 | 1.88E-50 | -1.69E-46 |
| MS.gene049915 | MS.gene50217 | 0.824168 | 9.34E-54 | -1.69E-46 |
| MS.gene050277 | MS.gene50217 | 0.809849 | 1.54E-50 | -1.69E-46 |
| MS.gene050303 | MS.gene50217 | 0.848174 | 7.25E-60 | -1.69E-46 |
| MS.gene050330 | MS.gene50217 | -0.830575 | 2.72E-55 | -1.69E-46 |
| MS.gene050642 | MS.gene50217 | 0.834405 | 3.05E-56 | -1.69E-46 |
| MS.gene051349 | MS.gene50217 | 0.810649 | 1.04E-50 | -1.69E-46 |
| MS.gene05137 | MS.gene50217 | 0.802052 | 6.74E-49 | -1.69E-46 |
| MS.gene051633 | MS.gene50217 | 0.822312 | 2.53E-53 | -1.69E-46 |
| MS.gene051662 | MS.gene50217 | 0.817301 | 3.54E-52 | -1.69E-46 |
| MS.gene051983 | MS.gene50217 | 0.808058 | 3.73E-50 | -1.69E-46 |
| MS.gene052176 | MS.gene50217 | 0.828551 | 8.44E-55 | -1.69E-46 |
| MS.gene052286 | MS.gene50217 | 0.822734 | 2.02E-53 | -1.69E-46 |
| MS.gene052302 | MS.gene50217 | 0.87628 | 1.58E-68 | -1.69E-46 |
| MS.gene052309 | MS.gene50217 | -0.841498 | 4.58E-58 | -1.69E-46 |
| MS.gene052376 | MS.gene50217 | 0.884094 | 2.57E-71 | -1.69E-46 |
| MS.gene052600 | MS.gene50217 | 0.836636 | 8.33E-57 | -1.69E-46 |
| MS.gene053836 | MS.gene50217 | 0.82347 | 1.36E-53 | -1.69E-46 |
| MS.gene053907 | MS.gene50217 | 0.828383 | 9.27E-55 | -1.69E-46 |
| MS.gene054667 | MS.gene50217 | 0.803816 | 2.91E-49 | -1.69E-46 |
| MS.gene054942 | MS.gene50217 | 0.836218 | 1.06E-56 | -1.69E-46 |
| MS.gene054983 | MS.gene50217 | 0.832761 | 7.86E-56 | -1.69E-46 |
| MS.gene056612 | MS.gene50217 | 0.80222 | 6.22E-49 | -1.69E-46 |
| MS.gene056631 | MS.gene50217 | 0.821741 | 3.44E-53 | -1.69E-46 |
| MS.gene056979 | MS.gene50217 | 0.801315 | 9.54E-49 | -1.69E-46 |
| MS.gene057079 | MS.gene50217 | 0.815205 | 1.04E-51 | -1.69E-46 |
| MS.gene057121 | MS.gene50217 | 0.800655 | 1.30E-48 | -1.69E-46 |
| MS.gene057260 | MS.gene50217 | 0.812567 | 3.97E-51 | -1.69E-46 |
| MS.gene057439 | MS.gene50217 | 0.820562 | 6.43E-53 | -1.69E-46 |
| MS.gene057754 | MS.gene50217 | 0.814294 | 1.66E-51 | -1.69E-46 |
| MS.gene058201 | MS.gene50217 | 0.824107 | 9.66E-54 | -1.69E-46 |
| MS.gene058749 | MS.gene50217 | 0.810725 | 9.99E-51 | -1.69E-46 |
| MS.gene06001 | MS.gene50217 | 0.804244 | 2.37E-49 | -1.69E-46 |
| MS.gene060476 | MS.gene50217 | 0.822718 | 2.04E-53 | -1.69E-46 |
| MS.gene061040 | MS.gene50217 | -0.800146 | 1.65E-48 | -1.69E-46 |
| MS.gene061049 | MS.gene50217 | 0.816777 | 4.64E-52 | -1.69E-46 |
| MS.gene061113 | MS.gene50217 | 0.808543 | 2.94E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50217.t1 | MTR_5g072780 | 93.333 | 180 | 9 | 1 | 1 | 177 | 1 | 180 | 8.00e-117 | 330 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50217.t1 | AT1G07050 | 46.914 | 81 | 32 | 4 | 103 | 177 | 82 | 157 | 6.29e-13 | 64.3 |
| MS.gene50217.t1 | AT1G07050 | 46.914 | 81 | 32 | 4 | 103 | 177 | 82 | 157 | 6.87e-13 | 64.3 |
Find 28 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTCATGTTCTTCTTTCTT+TGG | 0.183818 | 5.2:-60975050 | None:intergenic |
| GGTCGGACCGTGGTTCTCTT+TGG | 0.219465 | 5.2:+60975423 | MS.gene50217:CDS |
| GCTCTCTCTTCTTCCAATAT+TGG | 0.250193 | 5.2:-60976226 | None:intergenic |
| TTTGAACTATCAAGAGGTTT+TGG | 0.259887 | 5.2:+60975394 | MS.gene50217:CDS |
| GTCGGACCGTGGTTCTCTTT+GGG | 0.318029 | 5.2:+60975424 | MS.gene50217:CDS |
| GAAGAGAAGCAAGTGTTCTT+AGG | 0.336563 | 5.2:+60976251 | MS.gene50217:CDS |
| GATGGGAGAAGTGCCAATAT+TGG | 0.355037 | 5.2:+60976213 | MS.gene50217:intron |
| ATAGTAGTAATGAGCTTCAT+TGG | 0.401933 | 5.2:+60975216 | MS.gene50217:CDS |
| CTCATCTTTCCCTTCATCAA+AGG | 0.415253 | 5.2:-60975264 | None:intergenic |
| TCATCTTTCCCTTCATCAAA+GGG | 0.428053 | 5.2:-60975263 | None:intergenic |
| ACTAACATATGAGCCACTAT+TGG | 0.440550 | 5.2:-60975477 | None:intergenic |
| CATAAGCAAATGCAATTATG+AGG | 0.471927 | 5.2:+60975289 | MS.gene50217:CDS |
| TGAGCTTCATTGGGATATCA+TGG | 0.472007 | 5.2:+60975226 | MS.gene50217:CDS |
| GAAGGATTTCCCTTTGATGA+AGG | 0.484035 | 5.2:+60975254 | MS.gene50217:CDS |
| AAAAGAGAAAATAACAATGA+TGG | 0.501244 | 5.2:+60975326 | MS.gene50217:CDS |
| TAGTAGTAATGAGCTTCATT+GGG | 0.508412 | 5.2:+60975217 | MS.gene50217:CDS |
| AAGGATTTCCCTTTGATGAA+GGG | 0.518211 | 5.2:+60975255 | MS.gene50217:CDS |
| TCATTGGGATATCATGGAAT+GGG | 0.521421 | 5.2:+60975232 | MS.gene50217:CDS |
| AACATATGAGCCACTATTGG+AGG | 0.534168 | 5.2:-60975474 | None:intergenic |
| TTCATTGGGATATCATGGAA+TGG | 0.535775 | 5.2:+60975231 | MS.gene50217:CDS |
| GGTATTTGAGTGGTAGCGTC+TGG | 0.540746 | 5.2:-60975074 | None:intergenic |
| TTTGAATTTGAACTATCAAG+AGG | 0.551159 | 5.2:+60975388 | MS.gene50217:CDS |
| ATATGAGCCACTATTGGAGG+AGG | 0.606351 | 5.2:-60975471 | None:intergenic |
| TTGGATGCATGGTCGGACCG+TGG | 0.623784 | 5.2:+60975413 | MS.gene50217:CDS |
| TGGGATATCATGGAATGGGA+AGG | 0.627947 | 5.2:+60975236 | MS.gene50217:CDS |
| GAAGTTGGATGGTATTTGAG+TGG | 0.650148 | 5.2:-60975084 | None:intergenic |
| AGTGGATGATGAAAAGAGTG+TGG | 0.678709 | 5.2:+60975358 | MS.gene50217:CDS |
| CATTAGCCCAAAGAGAACCA+CGG | 0.726854 | 5.2:-60975430 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAATAATATAGTTGTTATA+TGG | - | chr5.2:60975631-60975650 | None:intergenic | 10.0% |
| !!! | AAATTTATTTTTTGTTTAGA+TGG | + | chr5.2:60976195-60976214 | MS.gene50217:intron | 10.0% |
| !!! | AATTTATTTTTTGTTTAGAT+GGG | + | chr5.2:60976196-60976215 | MS.gene50217:intron | 10.0% |
| !!! | TAATTTTAAATAGTAACAAA+AGG | - | chr5.2:60975663-60975682 | None:intergenic | 10.0% |
| !!! | TGTTTCTTTTTTTTTTTATT+GGG | + | chr5.2:60976401-60976420 | MS.gene50217:intron | 10.0% |
| !!! | TTGTTTCTTTTTTTTTTTAT+TGG | + | chr5.2:60976400-60976419 | MS.gene50217:intron | 10.0% |
| !!! | TATCAATTCTTATTCTTTTT+AGG | + | chr5.2:60975580-60975599 | MS.gene50217:intron | 15.0% |
| !! | AAAAGAGAAAATAACAATGA+TGG | + | chr5.2:60975326-60975345 | MS.gene50217:CDS | 20.0% |
| !! | ATATAACAACTTATCCAAAA+AGG | + | chr5.2:60975605-60975624 | MS.gene50217:intron | 20.0% |
| !! | TTATATATGAAAGTGAAGAT+TGG | - | chr5.2:60975512-60975531 | None:intergenic | 20.0% |
| !!! | AAAATAACAATGATGGTTTT+TGG | + | chr5.2:60975333-60975352 | MS.gene50217:CDS | 20.0% |
| !!! | AAATAACAATGATGGTTTTT+GGG | + | chr5.2:60975334-60975353 | MS.gene50217:CDS | 20.0% |
| !!! | ATAACTAAATTTTTGGTGAT+AGG | + | chr5.2:60975944-60975963 | MS.gene50217:intron | 20.0% |
| !!! | GTAACATATTTAGTTCATTT+TGG | + | chr5.2:60976040-60976059 | MS.gene50217:intron | 20.0% |
| !!! | TTTGTCGATAACTAAATTTT+TGG | + | chr5.2:60975937-60975956 | MS.gene50217:intron | 20.0% |
| !!! | TTTGTGATATTTTTGATGTA+GGG | + | chr5.2:60976461-60976480 | MS.gene50217:intron | 20.0% |
| !!! | TTTTGTGATATTTTTGATGT+AGG | + | chr5.2:60976460-60976479 | MS.gene50217:intron | 20.0% |
| !!! | TTTTTGGATAAGTTGTTATA+TGG | - | chr5.2:60975606-60975625 | None:intergenic | 20.0% |
| ! | TATTACTTAATTGACACCAA+CGG | - | chr5.2:60975807-60975826 | None:intergenic | 25.0% |
| ! | TTATTCGATCTATTCAATTG+TGG | + | chr5.2:60975908-60975927 | MS.gene50217:intron | 25.0% |
| ! | TTTGAATTTGAACTATCAAG+AGG | + | chr5.2:60975388-60975407 | MS.gene50217:CDS | 25.0% |
| !!! | AGTGTAAGATTTTTTGAAGT+TGG | - | chr5.2:60975102-60975121 | None:intergenic | 25.0% |
| !!! | TAAGATTTTTTGAAGTTGGA+TGG | - | chr5.2:60975098-60975117 | None:intergenic | 25.0% |
| !!! | TATGTTGACTTTTGTTTAGA+TGG | + | chr5.2:60975825-60975844 | MS.gene50217:intron | 25.0% |
| ACTTCATGTTCTTCTTTCTT+TGG | - | chr5.2:60975053-60975072 | None:intergenic | 30.0% | |
| ATAGTAGTAATGAGCTTCAT+TGG | + | chr5.2:60975216-60975235 | MS.gene50217:CDS | 30.0% | |
| ATTACTTAATTGACACCAAC+GGG | - | chr5.2:60975806-60975825 | None:intergenic | 30.0% | |
| CATAAGCAAATGCAATTATG+AGG | + | chr5.2:60975289-60975308 | MS.gene50217:CDS | 30.0% | |
| TAGTAGTAATGAGCTTCATT+GGG | + | chr5.2:60975217-60975236 | MS.gene50217:CDS | 30.0% | |
| TGATAAAAGACCAAGAATCA+AGG | + | chr5.2:60976342-60976361 | MS.gene50217:intron | 30.0% | |
| ! | CTACTATTACAAGTGTTTTG+TGG | - | chr5.2:60975203-60975222 | None:intergenic | 30.0% |
| ! | GCAAGAATAGTCATCAATTT+TGG | - | chr5.2:60975720-60975739 | None:intergenic | 30.0% |
| ! | TAGTTGTTATATGGCCTTTT+TGG | - | chr5.2:60975622-60975641 | None:intergenic | 30.0% |
| ! | TTTGAACTATCAAGAGGTTT+TGG | + | chr5.2:60975394-60975413 | MS.gene50217:CDS | 30.0% |
| AAGGATTTCCCTTTGATGAA+GGG | + | chr5.2:60975255-60975274 | MS.gene50217:CDS | 35.0% | |
| ACTAACATATGAGCCACTAT+TGG | - | chr5.2:60975480-60975499 | None:intergenic | 35.0% | |
| GATAGGTAATCTTTGATGCT+TGG | + | chr5.2:60975961-60975980 | MS.gene50217:intron | 35.0% | |
| TCATCTTTCCCTTCATCAAA+GGG | - | chr5.2:60975266-60975285 | None:intergenic | 35.0% | |
| TCATTGGGATATCATGGAAT+GGG | + | chr5.2:60975232-60975251 | MS.gene50217:CDS | 35.0% | |
| TTCATTGGGATATCATGGAA+TGG | + | chr5.2:60975231-60975250 | MS.gene50217:CDS | 35.0% | |
| ! | AAAAGTGTGACCTTGATTCT+TGG | - | chr5.2:60976355-60976374 | None:intergenic | 35.0% |
| !! | CAGCGTTTAATTTTCGAACT+TGG | - | chr5.2:60976324-60976343 | None:intergenic | 35.0% |
| AACATATGAGCCACTATTGG+AGG | - | chr5.2:60975477-60975496 | None:intergenic | 40.0% | |
| AACTCTTGTTGATCGCTTTC+TGG | + | chr5.2:60975854-60975873 | MS.gene50217:intron | 40.0% | |
| AGTGGATGATGAAAAGAGTG+TGG | + | chr5.2:60975358-60975377 | MS.gene50217:CDS | 40.0% | |
| CTCATCTTTCCCTTCATCAA+AGG | - | chr5.2:60975267-60975286 | None:intergenic | 40.0% | |
| GAAGAGAAGCAAGTGTTCTT+AGG | + | chr5.2:60976251-60976270 | MS.gene50217:CDS | 40.0% | |
| GAAGGATTTCCCTTTGATGA+AGG | + | chr5.2:60975254-60975273 | MS.gene50217:CDS | 40.0% | |
| GAAGTTGGATGGTATTTGAG+TGG | - | chr5.2:60975087-60975106 | None:intergenic | 40.0% | |
| GCTCTCTCTTCTTCCAATAT+TGG | - | chr5.2:60976229-60976248 | None:intergenic | 40.0% | |
| TGAGCTTCATTGGGATATCA+TGG | + | chr5.2:60975226-60975245 | MS.gene50217:CDS | 40.0% | |
| TTGATCGCTTTCTGGATGAT+AGG | + | chr5.2:60975862-60975881 | MS.gene50217:intron | 40.0% | |
| ! | CGCATAAAGATTTTACCCGT+TGG | + | chr5.2:60975788-60975807 | MS.gene50217:intron | 40.0% |
| ! | TCATTTTCCTCCTCCAATAG+TGG | + | chr5.2:60975464-60975483 | MS.gene50217:CDS | 40.0% |
| !! | CAATGATGGTTTTTGGGAAG+TGG | + | chr5.2:60975340-60975359 | MS.gene50217:CDS | 40.0% |
| !!! | ATCAAGAGGTTTTGGATGCA+TGG | + | chr5.2:60975402-60975421 | MS.gene50217:CDS | 40.0% |
| ATATGAGCCACTATTGGAGG+AGG | - | chr5.2:60975474-60975493 | None:intergenic | 45.0% | |
| CATTAGCCCAAAGAGAACCA+CGG | - | chr5.2:60975433-60975452 | None:intergenic | 45.0% | |
| TGGGATATCATGGAATGGGA+AGG | + | chr5.2:60975236-60975255 | MS.gene50217:CDS | 45.0% | |
| !! | GATGGGAGAAGTGCCAATAT+TGG | + | chr5.2:60976213-60976232 | MS.gene50217:intron | 45.0% |
| !!! | AGAGGTTTTGGATGCATGGT+CGG | + | chr5.2:60975406-60975425 | MS.gene50217:CDS | 45.0% |
| !!! | TAAACAAAAAATAAATTTTA+AGG | - | chr5.2:60976193-60976212 | None:intergenic | 5.0% |
| ! | GGTATTTGAGTGGTAGCGTC+TGG | - | chr5.2:60975077-60975096 | None:intergenic | 50.0% |
| GTCGGACCGTGGTTCTCTTT+GGG | + | chr5.2:60975424-60975443 | MS.gene50217:CDS | 55.0% | |
| GGTCGGACCGTGGTTCTCTT+TGG | + | chr5.2:60975423-60975442 | MS.gene50217:CDS | 60.0% | |
| TTGGATGCATGGTCGGACCG+TGG | + | chr5.2:60975413-60975432 | MS.gene50217:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 60975023 | 60976494 | 60975023 | ID=MS.gene50217 |
| chr5.2 | mRNA | 60975023 | 60976494 | 60975023 | ID=MS.gene50217.t1;Parent=MS.gene50217 |
| chr5.2 | exon | 60975023 | 60975493 | 60975023 | ID=MS.gene50217.t1.exon1;Parent=MS.gene50217.t1 |
| chr5.2 | CDS | 60975023 | 60975493 | 60975023 | ID=cds.MS.gene50217.t1;Parent=MS.gene50217.t1 |
| chr5.2 | exon | 60976214 | 60976272 | 60976214 | ID=MS.gene50217.t1.exon2;Parent=MS.gene50217.t1 |
| chr5.2 | CDS | 60976214 | 60976272 | 60976214 | ID=cds.MS.gene50217.t1;Parent=MS.gene50217.t1 |
| chr5.2 | exon | 60976482 | 60976494 | 60976482 | ID=MS.gene50217.t1.exon3;Parent=MS.gene50217.t1 |
| chr5.2 | CDS | 60976482 | 60976494 | 60976482 | ID=cds.MS.gene50217.t1;Parent=MS.gene50217.t1 |
| Gene Sequence |
| Protein sequence |
