AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56870


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56870.t1 MTR_4g105610 95.745 141 4 1 1 141 1 139 5.93e-92 267
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 30 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGTTTAAGGAGGCTAATTA+AGG 0.341665 8.1:-17580468 MS.gene56870:CDS
TTTGGCGGGTTTGCGGTTGA+TGG 0.351768 8.1:-17580805 MS.gene56870:CDS
GTTAAGTAGTACCAAGAGTT+TGG 0.384169 8.1:-17580823 MS.gene56870:CDS
AGCCTCCTTAAACGCAAAAC+AGG 0.417473 8.1:+17580476 None:intergenic
CCAAGAGTTTGGCGGGTTTG+CGG 0.430302 8.1:-17580812 MS.gene56870:CDS
CCGCAAACCCGCCAAACTCT+TGG 0.454081 8.1:+17580812 None:intergenic
TGCTCTCTTGAAACAACAAA+TGG 0.454342 8.1:-17580652 MS.gene56870:CDS
GGTGGCATGTAGTAATAATT+CGG 0.455634 8.1:-17580727 MS.gene56870:CDS
TTGGCGGGTTTGCGGTTGAT+GGG 0.472771 8.1:-17580804 MS.gene56870:CDS
GATAGAAATGAGGTTGAACT+TGG 0.475178 8.1:+17580690 None:intergenic
GCTTAAGTTGTTTGAAGAAG+AGG 0.484114 8.1:-17580501 MS.gene56870:CDS
GTTCAACCTCATTTCTATCT+CGG 0.485915 8.1:-17580686 MS.gene56870:CDS
AATTGCTGATGAGAGATTCC+AGG 0.487924 8.1:-17580441 MS.gene56870:intron
ATGAAGCGAGGGTGAAAAGA+AGG 0.496678 8.1:-17580779 MS.gene56870:CDS
TACCAACGTTGTAGCATCAC+TGG 0.515982 8.1:+17579319 None:intergenic
AAGTAGTACCAAGAGTTTGG+CGG 0.543463 8.1:-17580820 MS.gene56870:CDS
AGTAGTACCAAGAGTTTGGC+GGG 0.546540 8.1:-17580819 MS.gene56870:CDS
GGCATTGTTATAGGATTACA+AGG 0.561955 8.1:-17580546 MS.gene56870:intron
CAATAGCAATAGCATGGTGG+TGG 0.572915 8.1:-17580745 MS.gene56870:CDS
TGAAGCGAGGGTGAAAAGAA+GGG 0.587536 8.1:-17580778 MS.gene56870:CDS
TTGTTGTTTCAAGAGAGCAT+AGG 0.596200 8.1:+17580656 None:intergenic
TTCCAGTGATGCTACAACGT+TGG 0.604919 8.1:-17579321 MS.gene56870:CDS
GGTTGATGGGTCATGAAGCG+AGG 0.630103 8.1:-17580791 MS.gene56870:CDS
TTGAGTCCGAGATAGAAATG+AGG 0.635687 8.1:+17580680 None:intergenic
CAATAGCAATAGCAATAGCA+TGG 0.638289 8.1:-17580751 MS.gene56870:CDS
GAAGCGAGGGTGAAAAGAAG+GGG 0.645653 8.1:-17580777 MS.gene56870:CDS
GCGAGGGTGAAAAGAAGGGG+TGG 0.662555 8.1:-17580774 MS.gene56870:CDS
GTTGATGGGTCATGAAGCGA+GGG 0.668748 8.1:-17580790 MS.gene56870:CDS
GGAAGTAGCTGCTAAATCCG+AGG 0.672628 8.1:-17580631 MS.gene56870:intron
TAGCAATAGCAATAGCATGG+TGG 0.729970 8.1:-17580748 MS.gene56870:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAAAGTGTATTTTGAA+AGG + chr8.1:17579790-17579809 None:intergenic 15.0%
!!! AAAAAAAGTGTATTTTGAAA+GGG + chr8.1:17579789-17579808 None:intergenic 15.0%
!! AAATTTGAACTCTGTAAATA+TGG + chr8.1:17580503-17580522 None:intergenic 20.0%
!! AATGCATTACAATATTCAAT+AGG - chr8.1:17579868-17579887 MS.gene56870:intron 20.0%
!! ACTTAATTAAAAATTGTGTG+TGG - chr8.1:17579985-17580004 MS.gene56870:intron 20.0%
!! CTTAATTAAAAATTGTGTGT+GGG - chr8.1:17579986-17580005 MS.gene56870:intron 20.0%
!! TTAAGTGTACAAAAAATACA+AGG + chr8.1:17579971-17579990 None:intergenic 20.0%
!! TTTATTGAATTGTGAAACAT+AGG + chr8.1:17580561-17580580 None:intergenic 20.0%
! AATGAGTAAAAGCTATAGAT+TGG + chr8.1:17580450-17580469 None:intergenic 25.0%
! AATTAGAGATGAAATATGCA+TGG + chr8.1:17580682-17580701 None:intergenic 25.0%
! ACGCTTTATAATCTATTGTT+AGG + chr8.1:17579933-17579952 None:intergenic 25.0%
! TAAAGAAAATAATAGACACC+TGG + chr8.1:17579733-17579752 None:intergenic 25.0%
! TCAATTGTAGAATCTCAAAT+TGG - chr8.1:17579566-17579585 MS.gene56870:intron 25.0%
! TCGAGTGTACAAAAAATATA+AGG - chr8.1:17580035-17580054 MS.gene56870:intron 25.0%
! TTTACAGAGTTCAAATTTGT+TGG - chr8.1:17580505-17580524 MS.gene56870:CDS 25.0%
!!! ATTACTTTTGAAAACTACAC+CGG - chr8.1:17580175-17580194 MS.gene56870:intron 25.0%
!!! GAACATTTTAAAAAAGAGTG+TGG + chr8.1:17580815-17580834 None:intergenic 25.0%
ATCTTCTACAAAGAGTTCAA+AGG + chr8.1:17580235-17580254 None:intergenic 30.0%
GAAAAAGACAATACTAACCT+CGG + chr8.1:17579542-17579561 None:intergenic 30.0%
TCTTCTACAAAGAGTTCAAA+GGG + chr8.1:17580234-17580253 None:intergenic 30.0%
TCTTCTCAATAAGTTAGACA+GGG - chr8.1:17580471-17580490 MS.gene56870:CDS 30.0%
TGTAGAATCTCAAATTGGAA+TGG - chr8.1:17579571-17579590 MS.gene56870:intron 30.0%
! CAATTTGAGTATTGTGGAAT+AGG + chr8.1:17580378-17580397 None:intergenic 30.0%
! GGTGTAGTTTTCAAAAGTAA+TGG + chr8.1:17580176-17580195 None:intergenic 30.0%
! TCACATCAATTTGAGTATTG+TGG + chr8.1:17580384-17580403 None:intergenic 30.0%
!! AGTTCAAATTTGTTGGAGTA+TGG - chr8.1:17580512-17580531 MS.gene56870:CDS 30.0%
AATGCATTGTCCATACCATT+TGG + chr8.1:17579856-17579875 None:intergenic 35.0%
ATAGGATCACATAGACTGAT+AGG + chr8.1:17580543-17580562 None:intergenic 35.0%
ATGCATTGTCCATACCATTT+GGG + chr8.1:17579855-17579874 None:intergenic 35.0%
CAATAGCAATAGCAATAGCA+TGG - chr8.1:17579402-17579421 MS.gene56870:intron 35.0%
CTCTTCTCAATAAGTTAGAC+AGG - chr8.1:17580470-17580489 MS.gene56870:CDS 35.0%
GACAAAAAAAGAAACATGCG+AGG - chr8.1:17579897-17579916 MS.gene56870:intron 35.0%
GATAGAAATGAGGTTGAACT+TGG + chr8.1:17579466-17579485 None:intergenic 35.0%
GGCATTGTTATAGGATTACA+AGG - chr8.1:17579607-17579626 MS.gene56870:intron 35.0%
GGTGGCATGTAGTAATAATT+CGG - chr8.1:17579426-17579445 MS.gene56870:intron 35.0%
GTATCAAAGTTACACGAGAA+TGG - chr8.1:17580101-17580120 MS.gene56870:intron 35.0%
GTTAAGTAGTACCAAGAGTT+TGG - chr8.1:17579330-17579349 MS.gene56870:CDS 35.0%
GTTCAACCTCATTTCTATCT+CGG - chr8.1:17579467-17579486 MS.gene56870:intron 35.0%
TATGTCGATCTGATAGAATG+TGG + chr8.1:17580659-17580678 None:intergenic 35.0%
TGCTCTCTTGAAACAACAAA+TGG - chr8.1:17579501-17579520 MS.gene56870:intron 35.0%
TGTAATCCTATAACAATGCC+CGG + chr8.1:17579607-17579626 None:intergenic 35.0%
TGTAGAAGATTCACAACCTT+TGG - chr8.1:17580245-17580264 MS.gene56870:intron 35.0%
TTGGAATGGAAGATAAGAAC+CGG - chr8.1:17579585-17579604 MS.gene56870:intron 35.0%
! CACTCTTCCTTCTTATTTCT+AGG - chr8.1:17580751-17580770 MS.gene56870:CDS 35.0%
! CTAGTTGTGTTTGACACTTT+TGG + chr8.1:17580281-17580300 None:intergenic 35.0%
! GCTTAAGTTGTTTGAAGAAG+AGG - chr8.1:17579652-17579671 MS.gene56870:intron 35.0%
!! AATGGTTTTGCCATCCAAAA+AGG + chr8.1:17580158-17580177 None:intergenic 35.0%
!! ATGGTTTTGCCATCCAAAAA+GGG + chr8.1:17580157-17580176 None:intergenic 35.0%
!! TTGTTGTTTCAAGAGAGCAT+AGG + chr8.1:17579500-17579519 None:intergenic 35.0%
!! TTTGCTTAAAGCGGTTTTGA+TGG - chr8.1:17580349-17580368 MS.gene56870:intron 35.0%
AAGTAGTACCAAGAGTTTGG+CGG - chr8.1:17579333-17579352 MS.gene56870:CDS 40.0%
AATTGCTGATGAGAGATTCC+AGG - chr8.1:17579712-17579731 MS.gene56870:intron 40.0%
CAAAGTTACACGAGAATGGA+AGG - chr8.1:17580105-17580124 MS.gene56870:intron 40.0%
GCGTTTAAGGAGGCTAATTA+AGG - chr8.1:17579685-17579704 MS.gene56870:intron 40.0%
GCTGCATCCTAGAAATAAGA+AGG + chr8.1:17580761-17580780 None:intergenic 40.0%
GTACACTCGAGCTAAATTGT+TGG + chr8.1:17580025-17580044 None:intergenic 40.0%
TAACTCTCACCCAAATGGTA+TGG - chr8.1:17579843-17579862 MS.gene56870:intron 40.0%
TAGCAATAGCAATAGCATGG+TGG - chr8.1:17579405-17579424 MS.gene56870:intron 40.0%
TGCCATCCAAAAAGGGTTAA+TGG + chr8.1:17580150-17580169 None:intergenic 40.0%
TGGAATGGAAGATAAGAACC+GGG - chr8.1:17579586-17579605 MS.gene56870:intron 40.0%
TTCCAAGTAACATGTGAGTG+AGG - chr8.1:17579808-17579827 MS.gene56870:intron 40.0%
TTGAGTCCGAGATAGAAATG+AGG + chr8.1:17579476-17579495 None:intergenic 40.0%
! AACCTGTTTTGCGTTTAAGG+AGG - chr8.1:17579675-17579694 MS.gene56870:intron 40.0%
! AGGAACCTGTTTTGCGTTTA+AGG - chr8.1:17579672-17579691 MS.gene56870:intron 40.0%
! CAAAACCGCTTTAAGCAAAG+TGG + chr8.1:17580348-17580367 None:intergenic 40.0%
! TAAGAACCGGGCATTGTTAT+AGG - chr8.1:17579598-17579617 MS.gene56870:intron 40.0%
! TGCCATTAACCCTTTTTGGA+TGG - chr8.1:17580145-17580164 MS.gene56870:intron 40.0%
! TTTGGCCACTTTGCTTAAAG+CGG - chr8.1:17580340-17580359 MS.gene56870:intron 40.0%
!! AGTGTGCCATTAACCCTTTT+TGG - chr8.1:17580141-17580160 MS.gene56870:intron 40.0%
AAAACTACACCGGTAGACCT+AGG - chr8.1:17580185-17580204 MS.gene56870:intron 45.0%
AGCCTCCTTAAACGCAAAAC+AGG + chr8.1:17579680-17579699 None:intergenic 45.0%
AGTAGTACCAAGAGTTTGGC+GGG - chr8.1:17579334-17579353 MS.gene56870:CDS 45.0%
ATAGCAATGTTGCGTCTCCT+AGG + chr8.1:17580205-17580224 None:intergenic 45.0%
ATGAAGCGAGGGTGAAAAGA+AGG - chr8.1:17579374-17579393 MS.gene56870:CDS 45.0%
CAATAGCAATAGCATGGTGG+TGG - chr8.1:17579408-17579427 MS.gene56870:intron 45.0%
CACTCGAGCTAAATTGTTGG+AGG + chr8.1:17580022-17580041 None:intergenic 45.0%
CTCCTCACTCACATGTTACT+TGG + chr8.1:17579813-17579832 None:intergenic 45.0%
GAGCATAACTCTCACCCAAA+TGG - chr8.1:17579838-17579857 MS.gene56870:intron 45.0%
TACCAACGTTGTAGCATCAC+TGG + chr8.1:17580837-17580856 None:intergenic 45.0%
TGAACATGCTTTCTCGCGTT+TGG - chr8.1:17580322-17580341 MS.gene56870:intron 45.0%
TGAAGCGAGGGTGAAAAGAA+GGG - chr8.1:17579375-17579394 MS.gene56870:CDS 45.0%
TTCCAGTGATGCTACAACGT+TGG - chr8.1:17580832-17580851 MS.gene56870:CDS 45.0%
TTGGGTGAGAGTTATGCTCA+CGG + chr8.1:17579837-17579856 None:intergenic 45.0%
!! GATTCACAACCTTTGGTGCT+AGG - chr8.1:17580252-17580271 MS.gene56870:intron 45.0%
!!! TTTTGGCTTCCTAGCACCAA+AGG + chr8.1:17580264-17580283 None:intergenic 45.0%
ACACGAGAATGGAAGGCAAG+CGG - chr8.1:17580112-17580131 MS.gene56870:intron 50.0%
GAAGCGAGGGTGAAAAGAAG+GGG - chr8.1:17579376-17579395 MS.gene56870:CDS 50.0%
GGAAGTAGCTGCTAAATCCG+AGG - chr8.1:17579522-17579541 MS.gene56870:intron 50.0%
! GTTGATGGGTCATGAAGCGA+GGG - chr8.1:17579363-17579382 MS.gene56870:CDS 50.0%
CACGAGAATGGAAGGCAAGC+GGG - chr8.1:17580113-17580132 MS.gene56870:intron 55.0%
GTTGCGTCTCCTAGGTCTAC+CGG + chr8.1:17580197-17580216 None:intergenic 55.0%
!! CCAAGAGTTTGGCGGGTTTG+CGG - chr8.1:17579341-17579360 MS.gene56870:CDS 55.0%
!! GGTTGATGGGTCATGAAGCG+AGG - chr8.1:17579362-17579381 MS.gene56870:CDS 55.0%
!! TTGGCGGGTTTGCGGTTGAT+GGG - chr8.1:17579349-17579368 MS.gene56870:CDS 55.0%
!! TTTGGCGGGTTTGCGGTTGA+TGG - chr8.1:17579348-17579367 MS.gene56870:CDS 55.0%
CCGCAAACCCGCCAAACTCT+TGG + chr8.1:17579344-17579363 None:intergenic 60.0%
GCGAGGGTGAAAAGAAGGGG+TGG - chr8.1:17579379-17579398 MS.gene56870:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 17579316 17580859 17579316 ID=MS.gene56870
chr8.1 mRNA 17579316 17580859 17579316 ID=MS.gene56870.t1;Parent=MS.gene56870
chr8.1 exon 17580632 17580859 17580632 ID=MS.gene56870.t1.exon1;Parent=MS.gene56870.t1
chr8.1 CDS 17580632 17580859 17580632 ID=cds.MS.gene56870.t1;Parent=MS.gene56870.t1
chr8.1 exon 17580442 17580555 17580442 ID=MS.gene56870.t1.exon2;Parent=MS.gene56870.t1
chr8.1 CDS 17580442 17580555 17580442 ID=cds.MS.gene56870.t1;Parent=MS.gene56870.t1
chr8.1 exon 17579316 17579402 17579316 ID=MS.gene56870.t1.exon3;Parent=MS.gene56870.t1
chr8.1 CDS 17579316 17579402 17579316 ID=cds.MS.gene56870.t1;Parent=MS.gene56870.t1
Gene Sequence

>MS.gene56870

ATGCAATCGTTATCGTTAAGTAGTACCAAGAGTTTGGCGGGTTTGCGGTTGATGGGTCATGAAGCGAGGGTGAAAAGAAGGGGTGGCAATAGCAATAGCAATAGCATGGTGGTGGCATGTAGTAATAATTCGGATAAAGACACTGCTCCAAGTTCAACCTCATTTCTATCTCGGACTCAAACCTATGCTCTCTTGAAACAACAAATGGAAGTAGCTGCTAAATCCGAGGTTAGTATTGTCTTTTTCTTTATCAATTGTAGAATCTCAAATTGGAATGGAAGATAAGAACCGGGCATTGTTATAGGATTACAAGGAAGCTGCTAGAATTCGAGACTCGCTTAAGTTGTTTGAAGAAGAGGAACCTGTTTTGCGTTTAAGGAGGCTAATTAAGGAAGCAATTGCTGATGAGAGATTCCAGGTGTCTATTATTTTCTTTATTTAACAAAATTCAATGCTAATGATACTTTTTTCCCTTTCAAAATACACTTTTTTTTCCAAGTAACATGTGAGTGAGGAGTCCGTGAGCATAACTCTCACCCAAATGGTATGGACAATGCATTACAATATTCAATAGGCTACTTGACAAAAAAAGAAACATGCGAGGAAGAAACTCGCCTAACAATAGATTATAAAGCGTAGAATGTAGCGTGTTCCTTGTATTTTTTGTACACTTAATTAAAAATTGTGTGTGGGCTTTCACTATCCTCCAACAATTTAGCTCGAGTGTACAAAAAATATAAGGAACACGCTACATTTTACGCTTTATATAAATTAAGAAATGAAGAGTATCAAAGTTACACGAGAATGGAAGGCAAGCGGGCAACAAGTGTGCCATTAACCCTTTTTGGATGGCAAAACCATTACTTTTGAAAACTACACCGGTAGACCTAGGAGACGCAACATTGCTATGCATGTCCCTTTGAACTCTTTGTAGAAGATTCACAACCTTTGGTGCTAGGAAGCCAAAAGTGTCAAACACAACTAGTATAAAAGCATGTTGATTGTCTGAACATGCTTTCTCGCGTTTGGCCACTTTGCTTAAAGCGGTTTTGATGGTTGCCTATTCCACAATACTCAAATTGATGTGAAAGTTGCACATTGCAACTTCACTTGATACATCATTTCACAACACCAATCTATAGCTTTTACTCATTCTCTTCTCAATAAGTTAGACAGGGCAGATGCCATATTTACAGAGTTCAAATTTGTTGGAGTATGGCATTGCCTATCAGTCTATGTGATCCTATGTTTCACAATTCAATAAACTTTGTTTCTTTCAAGTTTCGACTTAGTCATTACATGTTGTAGTTAAGAACATACATCTATAGTCTTTCAGAATTCCACATTCTATCAGATCGACATACCATGCATATTTCATCTCTAATTGACAACACAAGATTTTTGTATTCTGACATATGATTTTACATGTTGATGACACTCTTCCTTCTTATTTCTAGGATGCAGCTAGTTATCGTGATGAGCTAAAAAGAATTGCTCCACACTCTTTTTTAAAATGTTCCAGTGATGCTACAACGTTGGTATGA

Protein sequence

>MS.gene56870.t1

MQSLSLSSTKSLAGLRLMGHEARVKRRGGNSNSNSMVVACSNNSDKDTAPSSTSFLSRTQTYALLKQQMEVAAKSEDYKEAARIRDSLKLFEEEEPVLRLRRLIKEAIADERFQDAASYRDELKRIAPHSFLKCSSDATTLV