AlfalfaGEDB | MS.gene60407

M. sativa cultivar XinJiangDaYe / MS.gene60407

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003680 GO:0003690 GO:0005488 GO:0043565 GO:0097159 GO:1901363 GO:1990837
PFAM: PF03479.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60407.t1	XP_003608618.2	99.7	296	1	0	1	296	1	296	7.30E-112	413.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60407.t1	O23620	83.5	212	25	2	85	296	91	292	6.3e-72	272.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60407.t1	G7JGG2	99.7	296	1	0	1	296	1	296	5.2e-112	413.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049222	MS.gene60407	-0.815575	8.62E-52	-1.69E-46
MS.gene049265	MS.gene60407	0.80692	6.51E-50	-1.69E-46
MS.gene049549	MS.gene60407	0.803965	2.71E-49	-1.69E-46
MS.gene049609	MS.gene60407	-0.809237	2.09E-50	-1.69E-46
MS.gene049634	MS.gene60407	0.803342	3.65E-49	-1.69E-46
MS.gene050380	MS.gene60407	0.802134	6.48E-49	-1.69E-46
MS.gene050675	MS.gene60407	-0.802128	6.50E-49	-1.69E-46
MS.gene050772	MS.gene60407	-0.812479	4.15E-51	-1.69E-46
MS.gene050809	MS.gene60407	0.80863	2.82E-50	-1.69E-46
MS.gene050950	MS.gene60407	-0.804005	2.66E-49	-1.69E-46
MS.gene051364	MS.gene60407	0.800478	1.41E-48	-1.69E-46
MS.gene051366	MS.gene60407	0.809832	1.56E-50	-1.69E-46
MS.gene051858	MS.gene60407	-0.807849	4.13E-50	-1.69E-46
MS.gene051907	MS.gene60407	0.80261	5.17E-49	-1.69E-46
MS.gene052211	MS.gene60407	0.803218	3.87E-49	-1.69E-46
MS.gene05240	MS.gene60407	0.813169	2.93E-51	-1.69E-46
MS.gene052615	MS.gene60407	-0.817447	3.28E-52	-1.69E-46
MS.gene053031	MS.gene60407	0.813888	2.04E-51	-1.69E-46
MS.gene053406	MS.gene60407	0.820964	5.19E-53	-1.69E-46
MS.gene053488	MS.gene60407	0.800958	1.13E-48	-1.69E-46
MS.gene054290	MS.gene60407	0.834533	2.84E-56	-1.69E-46
MS.gene055124	MS.gene60407	0.811265	7.63E-51	-1.69E-46
MS.gene055154	MS.gene60407	0.826329	2.88E-54	-1.69E-46
MS.gene055206	MS.gene60407	-0.808779	2.62E-50	-1.69E-46
MS.gene055502	MS.gene60407	-0.81376	2.17E-51	-1.69E-46
MS.gene056590	MS.gene60407	0.816272	6.02E-52	-1.69E-46
MS.gene056591	MS.gene60407	0.81414	1.79E-51	-1.69E-46
MS.gene056856	MS.gene60407	0.824583	7.46E-54	-1.69E-46
MS.gene057146	MS.gene60407	-0.800709	1.27E-48	-1.69E-46
MS.gene057544	MS.gene60407	0.807043	6.13E-50	-1.69E-46
MS.gene058130	MS.gene60407	-0.807001	6.25E-50	-1.69E-46
MS.gene058419	MS.gene60407	-0.803679	3.11E-49	-1.69E-46
MS.gene058603	MS.gene60407	0.816021	6.85E-52	-1.69E-46
MS.gene059536	MS.gene60407	-0.817965	2.51E-52	-1.69E-46
MS.gene059902	MS.gene60407	-0.805047	1.61E-49	-1.69E-46
MS.gene059955	MS.gene60407	0.811287	7.55E-51	-1.69E-46
MS.gene060818	MS.gene60407	-0.811833	5.75E-51	-1.69E-46
MS.gene061041	MS.gene60407	-0.821638	3.63E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60407.t1	MTR_4g098450	99.662	296	1	0	1	296	1	296	0.0	592
MS.gene60407.t1	MTR_7g080980	67.698	291	62	6	1	287	1	263	7.43e-100	294
MS.gene60407.t1	MTR_8g036060	51.877	293	122	3	19	296	20	308	3.48e-72	225
MS.gene60407.t1	MTR_5g091630	64.848	165	57	1	66	230	38	201	1.58e-65	206
MS.gene60407.t1	MTR_1g073860	59.296	199	72	2	72	261	90	288	6.89e-65	206
MS.gene60407.t1	MTR_1g044155	74.000	150	39	0	76	225	126	275	3.32e-64	206
MS.gene60407.t1	MTR_5g011520	72.152	158	44	0	72	229	96	253	3.80e-63	202
MS.gene60407.t1	MTR_3g068035	72.483	149	41	0	81	229	84	232	4.36e-62	199
MS.gene60407.t1	MTR_1g079810	63.095	168	62	0	76	243	74	241	3.85e-61	196
MS.gene60407.t1	MTR_3g100470	44.262	244	108	7	15	252	25	246	2.66e-56	183
MS.gene60407.t1	MTR_5g080580	64.828	145	51	0	81	225	94	238	1.29e-49	167
MS.gene60407.t1	MTR_5g066020	43.868	212	103	5	23	228	7	208	6.72e-44	150
MS.gene60407.t1	MTR_5g089600	37.584	149	82	2	76	213	77	225	2.12e-27	107
MS.gene60407.t1	MTR_5g010330	39.041	146	82	4	76	215	104	248	1.19e-23	98.6
MS.gene60407.t1	MTR_4g057487	34.177	158	94	3	76	223	17	174	1.61e-22	94.0
MS.gene60407.t1	MTR_4g057525	31.282	195	124	3	76	260	36	230	2.94e-22	93.2
MS.gene60407.t1	MTR_1g061530	39.007	141	82	2	106	242	144	284	4.16e-22	95.1
MS.gene60407.t1	MTR_5g089700	34.228	149	88	2	76	215	75	222	7.52e-22	92.0
MS.gene60407.t1	MTR_1g053685	32.335	167	104	2	76	233	85	251	1.03e-21	92.8
MS.gene60407.t1	MTR_6g061670	43.220	118	60	2	103	215	130	245	3.24e-21	92.0
MS.gene60407.t1	MTR_6g061670	43.220	118	60	2	103	215	125	240	3.89e-21	91.7
MS.gene60407.t1	MTR_1g032910	34.375	160	93	3	76	223	101	260	2.47e-20	89.4
MS.gene60407.t1	MTR_1g079780	38.261	115	70	1	103	216	109	223	5.03e-20	89.0
MS.gene60407.t1	MTR_5g089090	34.146	164	92	2	70	222	22	180	1.29e-19	85.5
MS.gene60407.t1	MTR_4g098460	36.538	156	82	4	68	215	49	195	5.71e-19	85.1
MS.gene60407.t1	MTR_1g076710	39.130	161	74	5	76	215	91	248	6.11e-19	85.9
MS.gene60407.t1	MTR_8g036160	45.614	114	58	1	106	215	145	258	1.56e-18	85.1
MS.gene60407.t1	MTR_1g073870	41.333	150	41	4	76	225	52	154	2.18e-18	82.4
MS.gene60407.t1	MTR_7g077120	38.514	148	81	3	76	215	127	272	6.12e-18	83.2
MS.gene60407.t1	MTR_4g098460	36.250	160	82	4	68	215	49	200	1.50e-17	81.3
MS.gene60407.t1	MTR_8g031100	40.127	157	86	3	67	215	114	270	2.93e-17	81.3
MS.gene60407.t1	MTR_7g074935	50.746	67	33	0	106	172	130	196	4.85e-17	80.5
MS.gene60407.t1	MTR_8g098390	34.307	137	86	3	95	228	158	293	5.82e-17	80.5
MS.gene60407.t1	MTR_7g116320	39.823	113	68	0	103	215	143	255	9.15e-17	80.1
MS.gene60407.t1	MTR_4g055803	39.167	120	67	2	102	215	124	243	2.98e-16	79.0
MS.gene60407.t1	MTR_4g055813	36.641	131	81	2	102	230	18	148	3.15e-16	77.4
MS.gene60407.t1	MTR_7g116320	39.200	125	72	1	103	223	143	267	3.62e-16	77.0
MS.gene60407.t1	MTR_5g011680	35.000	160	83	5	83	229	117	268	1.89e-15	75.5
MS.gene60407.t1	MTR_8g078930	34.328	134	78	3	107	231	5	137	8.56e-15	70.9
MS.gene60407.t1	MTR_4g101990	46.269	67	36	0	106	172	147	213	2.28e-14	72.8
MS.gene60407.t1	MTR_1g038960	33.333	195	111	6	77	256	125	315	3.38e-14	72.4
MS.gene60407.t1	MTR_4g055833	37.500	120	69	2	102	215	121	240	3.42e-14	72.4
MS.gene60407.t1	MTR_4g069110	33.333	102	68	0	69	170	27	128	3.64e-14	69.7
MS.gene60407.t1	MTR_5g011610	34.711	121	77	1	95	215	50	168	1.35e-13	70.5
MS.gene60407.t1	MTR_5g011620	34.783	115	73	1	101	215	113	225	1.49e-13	71.2
MS.gene60407.t1	MTR_6g060670	41.441	111	63	2	106	216	180	288	4.58e-13	68.6
MS.gene60407.t1	MTR_1g071850	34.507	142	65	3	84	225	38	151	4.56e-12	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60407.t1	AT4G17800	63.208	318	69	8	1	296	1	292	3.62e-94	280
MS.gene60407.t1	AT2G35270	57.372	312	90	7	1	296	1	285	3.27e-93	278
MS.gene60407.t1	AT4G14465	52.016	248	105	5	27	272	24	259	7.27e-66	207
MS.gene60407.t1	AT1G76500	59.239	184	66	1	53	227	49	232	7.80e-64	203
MS.gene60407.t1	AT2G45430	58.333	228	78	4	74	286	87	312	3.63e-62	199
MS.gene60407.t1	AT2G42940	62.179	156	59	0	74	229	51	206	2.05e-61	196
MS.gene60407.t1	AT3G60870	54.680	203	69	4	23	220	17	201	5.27e-61	195
MS.gene60407.t1	AT4G12050	69.811	159	47	1	74	231	116	274	2.60e-60	195
MS.gene60407.t1	AT4G22810	70.807	161	44	1	74	231	103	263	5.74e-60	194
MS.gene60407.t1	AT3G04570	66.456	158	45	1	76	225	80	237	2.72e-58	189
MS.gene60407.t1	AT4G35390	50.538	279	121	3	9	276	2	274	2.89e-58	189
MS.gene60407.t1	AT5G49700	56.442	163	64	2	74	229	54	216	3.16e-57	185
MS.gene60407.t1	AT1G20900	53.211	218	62	2	17	225	58	244	3.44e-49	166
MS.gene60407.t1	AT3G55560	55.500	200	87	2	81	278	93	292	3.23e-47	160
MS.gene60407.t1	AT1G14490	46.961	181	84	4	77	247	6	184	2.80e-39	137
MS.gene60407.t1	AT1G14490	45.408	196	94	5	62	247	11	203	3.43e-39	137
MS.gene60407.t1	AT3G04590	34.225	187	104	4	98	282	166	335	6.48e-23	97.8
MS.gene60407.t1	AT2G33620	42.361	144	79	3	75	215	136	278	7.19e-23	97.1
MS.gene60407.t1	AT2G33620	42.361	144	79	3	75	215	136	278	7.19e-23	97.1
MS.gene60407.t1	AT2G33620	42.361	144	79	3	75	215	136	278	7.19e-23	97.1
MS.gene60407.t1	AT2G33620	42.361	144	79	3	75	215	136	278	7.19e-23	97.1
MS.gene60407.t1	AT5G62260	42.857	126	65	2	103	223	160	283	8.87e-23	95.9
MS.gene60407.t1	AT5G62260	45.763	118	57	2	103	215	160	275	9.40e-23	97.4
MS.gene60407.t1	AT5G62260	45.763	118	57	2	103	215	160	275	9.40e-23	97.4
MS.gene60407.t1	AT1G63470	40.268	149	76	5	76	215	147	291	1.26e-22	96.7
MS.gene60407.t1	AT4G12080	44.348	115	57	2	106	215	173	285	1.28e-22	96.7
MS.gene60407.t1	AT5G51590	42.017	119	62	2	102	215	177	293	5.46e-22	95.5
MS.gene60407.t1	AT2G45850	36.257	171	97	4	76	236	132	300	5.70e-22	94.7
MS.gene60407.t1	AT2G45850	36.257	171	97	4	76	236	132	300	5.70e-22	94.7
MS.gene60407.t1	AT3G04590	37.415	147	75	2	106	252	174	303	6.10e-22	94.0
MS.gene60407.t1	AT4G17950	40.385	156	86	3	65	215	185	338	4.46e-21	92.8
MS.gene60407.t1	AT4G25320	39.098	133	72	3	106	235	169	295	5.81e-21	92.4
MS.gene60407.t1	AT2G45850	36.913	149	84	3	76	216	132	278	5.48e-20	87.8
MS.gene60407.t1	AT3G61310	31.163	215	128	4	76	284	134	334	5.39e-18	83.6
MS.gene60407.t1	AT5G46640	43.226	155	79	4	76	226	155	304	3.62e-17	81.3
MS.gene60407.t1	AT4G00200	42.478	113	62	1	106	215	126	238	7.32e-16	77.0
MS.gene60407.t1	AT4G00200	42.478	113	62	1	106	215	126	238	7.32e-16	77.0
MS.gene60407.t1	AT1G63480	29.231	195	111	7	76	247	130	320	4.25e-15	75.1
MS.gene60407.t1	AT1G63480	29.231	195	111	7	76	247	130	320	4.25e-15	75.1
MS.gene60407.t1	AT1G63480	29.231	195	111	7	76	247	124	314	4.42e-15	75.1
MS.gene60407.t1	AT1G63480	29.231	195	111	7	76	247	124	314	4.42e-15	75.1
MS.gene60407.t1	AT1G63480	29.231	195	111	7	76	247	124	314	4.42e-15	75.1
MS.gene60407.t1	AT1G63480	29.231	195	111	7	76	247	124	314	4.42e-15	75.1
MS.gene60407.t1	AT1G63480	29.231	195	111	7	76	247	124	314	4.42e-15	75.1
MS.gene60407.t1	AT4G22770	43.243	74	42	0	99	172	146	219	2.22e-14	72.8
MS.gene60407.t1	AT4G22770	43.243	74	42	0	99	172	146	219	2.22e-14	72.8

Find 100 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGATGGCTGGGTTAGATTT+AGG	0.137320	8.1:-24589550	MS.gene60407:CDS
CCATTTCCTGATCCATCTTC+TGG	0.220564	8.1:-24588785	MS.gene60407:CDS
CTCTCTATTACTTTGATGGC+TGG	0.242004	8.1:-24589562	None:intergenic
TGATGGCTGGGTTAGATTTA+GGG	0.282094	8.1:-24589549	MS.gene60407:CDS
CTTCAGAATCGTGCTGGTTT+TGG	0.296798	8.1:+24589464	None:intergenic
TTAATTCTAAATCCTCAAAA+AGG	0.306047	8.1:+24588665	None:intergenic
CGCAGCCAAGGTTTGGAGCT+TGG	0.329216	8.1:-24589376	MS.gene60407:CDS
GTTCATCAGTTGCCTGTTGA+TGG	0.350083	8.1:-24588725	MS.gene60407:CDS
GGCTCCGGTGGTGGTGGTGG+CGG	0.351891	8.1:-24588824	MS.gene60407:CDS
CTTCAGATGCAGGCTGAGGC+TGG	0.363407	8.1:-24588860	MS.gene60407:CDS
TTTGAGATATTGTCCTTGTC+TGG	0.371554	8.1:-24589067	MS.gene60407:CDS
CACCAGCCGCCGCTGGTTGC+CGG	0.375766	8.1:+24589113	None:intergenic
GGTGATGGTGTTGTTGTTGA+AGG	0.376453	8.1:+24589485	None:intergenic
AGCTTAACGATTTATCTTGC+TGG	0.384930	8.1:-24589007	MS.gene60407:CDS
CAAGGCTCCGGTGGTGGTGG+TGG	0.398851	8.1:-24588827	MS.gene60407:CDS
AGTGATGATCACCGGTGGTT+TGG	0.402875	8.1:+24589282	None:intergenic
AGATGATCGCAGCCAAGGTT+TGG	0.404818	8.1:-24589383	MS.gene60407:CDS
CCAGAAGATGGATCAGGAAA+TGG	0.417544	8.1:+24588785	None:intergenic
TTGGAGCTTGGTTCAGCAGC+CGG	0.422838	8.1:-24589364	MS.gene60407:CDS
GGTTCTCAAGGCTCCGGTGG+TGG	0.425813	8.1:-24588833	MS.gene60407:CDS
TCTCTATTACTTTGATGGCT+GGG	0.430077	8.1:-24589561	None:intergenic
TCTCAAGGCTCCGGTGGTGG+TGG	0.432039	8.1:-24588830	MS.gene60407:CDS
TCAGTTGCCTGTTGATGGTT+GGG	0.432684	8.1:-24588720	MS.gene60407:CDS
CTTGTTCTTCAGAATCGTGC+TGG	0.433912	8.1:+24589458	None:intergenic
CCGTCCACGCGGCAGACCAC+CGG	0.439648	8.1:-24589320	MS.gene60407:CDS
GTTGGTGAGCTTATCGCAGC+CGG	0.441179	8.1:-24588953	MS.gene60407:CDS
ATCAGTTGCCTGTTGATGGT+TGG	0.441577	8.1:-24588721	MS.gene60407:CDS
TTTCCCGCCCAACCATCAAC+AGG	0.442066	8.1:+24588713	None:intergenic
CTGCCACCACCAGCTCCTCC+CGG	0.457572	8.1:-24589037	MS.gene60407:CDS
CTAGTTGCTCCGGGAGGAGC+TGG	0.461233	8.1:+24589028	None:intergenic
GCCTACCACGTCTCCTGGTC+CGG	0.473561	8.1:+24589345	None:intergenic
TGAGAAGTTGCCGTTGGAAG+AGG	0.475190	8.1:-24588888	MS.gene60407:CDS
CTTGGTTCAGCAGCCGGACC+AGG	0.477069	8.1:-24589358	MS.gene60407:CDS
AATCGTTAAGCTAGTTGCTC+CGG	0.477146	8.1:+24589018	None:intergenic
CCGGTGGTCTGCCGCGTGGA+CGG	0.481538	8.1:+24589320	None:intergenic
ATCCGGCAACCAGCGGCGGC+TGG	0.482044	8.1:-24589115	MS.gene60407:CDS
AGAGGAGCAGCTTCAGATGC+AGG	0.483216	8.1:-24588870	MS.gene60407:CDS
GAGGCTGGAGGAGGTTCTCA+AGG	0.498113	8.1:-24588845	MS.gene60407:CDS
ATCTGTGTTCTTAGCGGCAG+TGG	0.498629	8.1:-24589157	MS.gene60407:CDS
GCTCCGGGAGGAGCTGGTGG+TGG	0.501296	8.1:+24589034	None:intergenic
CTGTGACAAATGTCAGTATC+CGG	0.502662	8.1:-24589132	MS.gene60407:CDS
CGGCGGCCTACCACGTCTCC+TGG	0.502985	8.1:+24589340	None:intergenic
CGGCAACCAGCGGCGGCTGG+TGG	0.511641	8.1:-24589112	MS.gene60407:CDS
GGAGGAGGTTCTCAAGGCTC+CGG	0.514652	8.1:-24588839	MS.gene60407:CDS
GGTGGTGTTGTTACGCTACA+TGG	0.516870	8.1:-24589094	MS.gene60407:CDS
GTGGTGGGAGGTAGCGTGGT+TGG	0.517362	8.1:-24588971	MS.gene60407:CDS
GCAGCTTCAGATGCAGGCTG+AGG	0.522442	8.1:-24588864	MS.gene60407:CDS
CGGTGCACCACCACCAACAC+TGG	0.523693	8.1:+24589423	None:intergenic
ATTTATCTTGCTGGAGGACA+AGG	0.523860	8.1:-24588998	MS.gene60407:CDS
GAAGCTGCAATCACAATCAC+TGG	0.524358	8.1:+24588929	None:intergenic
CGTGGGATCTGTGTTCTTAG+CGG	0.526507	8.1:-24589163	MS.gene60407:CDS
CTGAACCAAGCTCCAAACCT+TGG	0.527609	8.1:+24589371	None:intergenic
TGAACATTCTGCATATTCAA+TGG	0.529701	8.1:+24588743	None:intergenic
TTCAAGAATATGAGCTCTGA+GGG	0.530656	8.1:+24589243	None:intergenic
TGGAGGACAAGGACAGGTGG+TGG	0.534409	8.1:-24588987	MS.gene60407:CDS
GGTAAACCAGAAGATGGATC+AGG	0.535636	8.1:+24588779	None:intergenic
CTCTCTCGAGTGATGATCAC+CGG	0.539638	8.1:+24589274	None:intergenic
ATGCAGGCTGAGGCTGGAGG+AGG	0.542838	8.1:-24588854	MS.gene60407:CDS
ATTGCCGCCACCACCACCAC+CGG	0.545968	8.1:+24588820	None:intergenic
ATCGTTAAGCTAGTTGCTCC+GGG	0.547154	8.1:+24589019	None:intergenic
GGAGGACAAGGACAGGTGGT+GGG	0.551835	8.1:-24588986	MS.gene60407:CDS
ACAACACCACCAGCCGCCGC+TGG	0.553782	8.1:+24589106	None:intergenic
TTGCCTGTTGATGGTTGGGC+GGG	0.555085	8.1:-24588716	MS.gene60407:CDS
TGCTTATGAGAAGTTGCCGT+TGG	0.557657	8.1:-24588894	MS.gene60407:CDS
GTGGTCTGCCGCGTGGACGG+CGG	0.559279	8.1:+24589323	None:intergenic
GTTGCTCCGGGAGGAGCTGG+TGG	0.563676	8.1:+24589031	None:intergenic
TGCAATCACAATCACTGGCC+CGG	0.564705	8.1:+24588934	None:intergenic
GAATCAAATCGTGCCAGTGT+TGG	0.565155	8.1:-24589436	MS.gene60407:CDS
ACGTTGACGACGGCGAGCAT+AGG	0.566838	8.1:+24589183	None:intergenic
GGAGGTTCTCAAGGCTCCGG+TGG	0.570585	8.1:-24588836	MS.gene60407:CDS
CGTCCACGCGGCAGACCACC+GGG	0.573105	8.1:-24589319	MS.gene60407:CDS
TTGGTGAGCTTATCGCAGCC+GGG	0.573283	8.1:-24588952	MS.gene60407:CDS
TCTTGCTGGAGGACAAGGAC+AGG	0.576199	8.1:-24588993	MS.gene60407:CDS
TCTTCTTCGTTCGATGAGAA+CGG	0.581747	8.1:+24589403	None:intergenic
GCTTTATGCAAAACATCCAC+AGG	0.581801	8.1:-24589519	MS.gene60407:CDS
TCAAATCGTGCCAGTGTTGG+TGG	0.581951	8.1:-24589433	MS.gene60407:CDS
ACAGGTGGTGGGAGGTAGCG+TGG	0.584119	8.1:-24588975	MS.gene60407:CDS
CAGATGCAGGCTGAGGCTGG+AGG	0.594179	8.1:-24588857	MS.gene60407:CDS
GAAGAAGATGATCGCAGCCA+AGG	0.594535	8.1:-24589388	MS.gene60407:CDS
TTAACGATTTATCTTGCTGG+AGG	0.597516	8.1:-24589004	MS.gene60407:CDS
TTGACGACGGCGAGCATAGG+TGG	0.600696	8.1:+24589186	None:intergenic
CAGTATCCGGCAACCAGCGG+CGG	0.607370	8.1:-24589119	MS.gene60407:CDS
CATATTCTTGAAGTTGCCAG+TGG	0.608398	8.1:-24589232	MS.gene60407:CDS
AATCGTGCCAGTGTTGGTGG+TGG	0.611425	8.1:-24589430	MS.gene60407:CDS
TATGCTCGCCGTCGTCAACG+TGG	0.615533	8.1:-24589181	MS.gene60407:CDS
CACAGATCCCACGTTGACGA+CGG	0.619869	8.1:+24589173	None:intergenic
AGCAGCCGGACCAGGAGACG+TGG	0.625149	8.1:-24589350	MS.gene60407:CDS
GGACAAGGACAGGTGGTGGG+AGG	0.626108	8.1:-24588983	MS.gene60407:CDS
TGAAGCTGCTCCTCTTCCAA+CGG	0.628906	8.1:+24588878	None:intergenic
ATGCTCGCCGTCGTCAACGT+GGG	0.633340	8.1:-24589180	MS.gene60407:CDS
TGTCAGTATCCGGCAACCAG+CGG	0.635215	8.1:-24589122	MS.gene60407:CDS
GTTGCCTGTTGATGGTTGGG+CGG	0.635915	8.1:-24588717	MS.gene60407:CDS
TGCTGGAGGACAAGGACAGG+TGG	0.640269	8.1:-24588990	MS.gene60407:CDS
CTTCAAGAATATGAGCTCTG+AGG	0.644966	8.1:+24589242	None:intergenic
GTGGTAGGCCGCCGTCCACG+CGG	0.646690	8.1:-24589331	MS.gene60407:CDS
TCTCGAGTGATGATCACCGG+TGG	0.659633	8.1:+24589277	None:intergenic
GCCGGACCAGGAGACGTGGT+AGG	0.693052	8.1:-24589346	MS.gene60407:CDS
GATCCCGGTGGTCTGCCGCG+TGG	0.697134	8.1:+24589316	None:intergenic
TGGCAGAAAAGATCCAGACA+AGG	0.722466	8.1:+24589054	None:intergenic
GTTAAGCTAGTTGCTCCGGG+AGG	0.738557	8.1:+24589022	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTCAATGGCAAATTGAAAAA+CGG	+	chr8.1:24589471-24589490	None:intergenic	25.0%
	TGAACATTCTGCATATTCAA+TGG	+	chr8.1:24589486-24589505	None:intergenic	30.0%
	AAAAACGGTAAACCAGAAGA+TGG	+	chr8.1:24589456-24589475	None:intergenic	35.0%
	AGCTTAACGATTTATCTTGC+TGG	-	chr8.1:24589219-24589238	MS.gene60407:CDS	35.0%
	TTAACGATTTATCTTGCTGG+AGG	-	chr8.1:24589222-24589241	MS.gene60407:CDS	35.0%
	TTCAAAAACATCAGAACCAC+TGG	+	chr8.1:24589013-24589032	None:intergenic	35.0%
!	TTCAAGAATATGAGCTCTGA+GGG	+	chr8.1:24588986-24589005	None:intergenic	35.0%
!	TTTGAGATATTGTCCTTGTC+TGG	-	chr8.1:24589159-24589178	MS.gene60407:CDS	35.0%
	AATCGTTAAGCTAGTTGCTC+CGG	+	chr8.1:24589211-24589230	None:intergenic	40.0%
	ATTTATCTTGCTGGAGGACA+AGG	-	chr8.1:24589228-24589247	MS.gene60407:CDS	40.0%
	CATATTCTTGAAGTTGCCAG+TGG	-	chr8.1:24588994-24589013	MS.gene60407:CDS	40.0%
	CTGTGACAAATGTCAGTATC+CGG	-	chr8.1:24589094-24589113	MS.gene60407:CDS	40.0%
	CTTCAAGAATATGAGCTCTG+AGG	+	chr8.1:24588987-24589006	None:intergenic	40.0%
	GAAAAACAAAGCCAAACCAC+CGG	-	chr8.1:24588933-24588952	MS.gene60407:CDS	40.0%
	GCTTTATGCAAAACATCCAC+AGG	-	chr8.1:24588707-24588726	MS.gene60407:CDS	40.0%
	TCTTCTTCGTTCGATGAGAA+CGG	+	chr8.1:24588826-24588845	None:intergenic	40.0%
!!!	TTGGCTTTGTTTTTCGATCC+CGG	+	chr8.1:24588928-24588947	None:intergenic	40.0%
	ATCGTTAAGCTAGTTGCTCC+GGG	+	chr8.1:24589210-24589229	None:intergenic	45.0%
	CCAGAAGATGGATCAGGAAA+TGG	+	chr8.1:24589444-24589463	None:intergenic	45.0%
	CCATTTCCTGATCCATCTTC+TGG	-	chr8.1:24589441-24589460	MS.gene60407:CDS	45.0%
	CTTGTTCTTCAGAATCGTGC+TGG	+	chr8.1:24588771-24588790	None:intergenic	45.0%
	GAAGCTGCAATCACAATCAC+TGG	+	chr8.1:24589300-24589319	None:intergenic	45.0%
	GAATCAAATCGTGCCAGTGT+TGG	-	chr8.1:24588790-24588809	MS.gene60407:CDS	45.0%
	GGTAAACCAGAAGATGGATC+AGG	+	chr8.1:24589450-24589469	None:intergenic	45.0%
	GTTCATCAGTTGCCTGTTGA+TGG	-	chr8.1:24589501-24589520	MS.gene60407:CDS	45.0%
	TGCTTATGAGAAGTTGCCGT+TGG	-	chr8.1:24589332-24589351	MS.gene60407:CDS	45.0%
	TGGCAGAAAAGATCCAGACA+AGG	+	chr8.1:24589175-24589194	None:intergenic	45.0%
!!	ATCAGTTGCCTGTTGATGGT+TGG	-	chr8.1:24589505-24589524	MS.gene60407:CDS	45.0%
!!	GAAGGTGTAAGTTTTGCCTG+TGG	+	chr8.1:24588726-24588745	None:intergenic	45.0%
!!	GGTGATGGTGTTGTTGTTGA+AGG	+	chr8.1:24588744-24588763	None:intergenic	45.0%
!!	TCAGTTGCCTGTTGATGGTT+GGG	-	chr8.1:24589506-24589525	MS.gene60407:CDS	45.0%
!!!	AATCGTGCTGGTTTTGGTGA+TGG	+	chr8.1:24588759-24588778	None:intergenic	45.0%
!!!	CTTCAGAATCGTGCTGGTTT+TGG	+	chr8.1:24588765-24588784	None:intergenic	45.0%
	AGATGATCGCAGCCAAGGTT+TGG	-	chr8.1:24588843-24588862	MS.gene60407:CDS	50.0%
	AGTGATGATCACCGGTGGTT+TGG	+	chr8.1:24588947-24588966	None:intergenic	50.0%
	ATCTGTGTTCTTAGCGGCAG+TGG	-	chr8.1:24589069-24589088	MS.gene60407:CDS	50.0%
	CGTGGGATCTGTGTTCTTAG+CGG	-	chr8.1:24589063-24589082	MS.gene60407:CDS	50.0%
	CTCTCTCGAGTGATGATCAC+CGG	+	chr8.1:24588955-24588974	None:intergenic	50.0%
	CTGAACCAAGCTCCAAACCT+TGG	+	chr8.1:24588858-24588877	None:intergenic	50.0%
	GAAGAAGATGATCGCAGCCA+AGG	-	chr8.1:24588838-24588857	MS.gene60407:CDS	50.0%
	TGAAGCTGCTCCTCTTCCAA+CGG	+	chr8.1:24589351-24589370	None:intergenic	50.0%
	TGAGAAGTTGCCGTTGGAAG+AGG	-	chr8.1:24589338-24589357	MS.gene60407:CDS	50.0%
	TGCAATCACAATCACTGGCC+CGG	+	chr8.1:24589295-24589314	None:intergenic	50.0%
!	TCAAATCGTGCCAGTGTTGG+TGG	-	chr8.1:24588793-24588812	MS.gene60407:CDS	50.0%
!!	GGTGGTGTTGTTACGCTACA+TGG	-	chr8.1:24589132-24589151	MS.gene60407:CDS	50.0%
!!!	GCTTTGTTTTTCGATCCCGG+TGG	+	chr8.1:24588925-24588944	None:intergenic	50.0%
	AGAGGAGCAGCTTCAGATGC+AGG	-	chr8.1:24589356-24589375	MS.gene60407:CDS	55.0%
	CACAGATCCCACGTTGACGA+CGG	+	chr8.1:24589056-24589075	None:intergenic	55.0%
	GTTAAGCTAGTTGCTCCGGG+AGG	+	chr8.1:24589207-24589226	None:intergenic	55.0%
	TCTCGAGTGATGATCACCGG+TGG	+	chr8.1:24588952-24588971	None:intergenic	55.0%
	TCTTGCTGGAGGACAAGGAC+AGG	-	chr8.1:24589233-24589252	MS.gene60407:CDS	55.0%
	TGTCAGTATCCGGCAACCAG+CGG	-	chr8.1:24589104-24589123	MS.gene60407:CDS	55.0%
	TTGGAGCTTGGTTCAGCAGC+CGG	-	chr8.1:24588862-24588881	MS.gene60407:CDS	55.0%
	TTGGTGAGCTTATCGCAGCC+GGG	-	chr8.1:24589274-24589293	MS.gene60407:CDS	55.0%
	TTTCCCGCCCAACCATCAAC+AGG	+	chr8.1:24589516-24589535	None:intergenic	55.0%
!	GTTGGTGAGCTTATCGCAGC+CGG	-	chr8.1:24589273-24589292	MS.gene60407:CDS	55.0%
!!	AATCGTGCCAGTGTTGGTGG+TGG	-	chr8.1:24588796-24588815	MS.gene60407:CDS	55.0%
!!	GTTGCCTGTTGATGGTTGGG+CGG	-	chr8.1:24589509-24589528	MS.gene60407:CDS	55.0%
!!	TTGCCTGTTGATGGTTGGGC+GGG	-	chr8.1:24589510-24589529	MS.gene60407:CDS	55.0%
	ACGTTGACGACGGCGAGCAT+AGG	+	chr8.1:24589046-24589065	None:intergenic	60.0%
	ATGCTCGCCGTCGTCAACGT+GGG	-	chr8.1:24589046-24589065	MS.gene60407:CDS	60.0%
	CTTCAGATGCAGGCTGAGGC+TGG	-	chr8.1:24589366-24589385	MS.gene60407:CDS	60.0%
	GAGGCTGGAGGAGGTTCTCA+AGG	-	chr8.1:24589381-24589400	MS.gene60407:CDS	60.0%
	GCAGCTTCAGATGCAGGCTG+AGG	-	chr8.1:24589362-24589381	MS.gene60407:CDS	60.0%
	GGAGGACAAGGACAGGTGGT+GGG	-	chr8.1:24589240-24589259	MS.gene60407:CDS	60.0%
	GGAGGAGGTTCTCAAGGCTC+CGG	-	chr8.1:24589387-24589406	MS.gene60407:CDS	60.0%
	TATGCTCGCCGTCGTCAACG+TGG	-	chr8.1:24589045-24589064	MS.gene60407:CDS	60.0%
	TGCTGGAGGACAAGGACAGG+TGG	-	chr8.1:24589236-24589255	MS.gene60407:CDS	60.0%
	TGGAGGACAAGGACAGGTGG+TGG	-	chr8.1:24589239-24589258	MS.gene60407:CDS	60.0%
	TTGACGACGGCGAGCATAGG+TGG	+	chr8.1:24589043-24589062	None:intergenic	60.0%
!	CGCAGCCAAGGTTTGGAGCT+TGG	-	chr8.1:24588850-24588869	MS.gene60407:CDS	60.0%
	ATGCAGGCTGAGGCTGGAGG+AGG	-	chr8.1:24589372-24589391	MS.gene60407:CDS	65.0%
	ATTGCCGCCACCACCACCAC+CGG	+	chr8.1:24589409-24589428	None:intergenic	65.0%
	CAGATGCAGGCTGAGGCTGG+AGG	-	chr8.1:24589369-24589388	MS.gene60407:CDS	65.0%
	CAGTATCCGGCAACCAGCGG+CGG	-	chr8.1:24589107-24589126	MS.gene60407:CDS	65.0%
	CGGTGCACCACCACCAACAC+TGG	+	chr8.1:24588806-24588825	None:intergenic	65.0%
	CTAGTTGCTCCGGGAGGAGC+TGG	+	chr8.1:24589201-24589220	None:intergenic	65.0%
	CTTGGTTCAGCAGCCGGACC+AGG	-	chr8.1:24588868-24588887	MS.gene60407:CDS	65.0%
	GCCTACCACGTCTCCTGGTC+CGG	+	chr8.1:24588884-24588903	None:intergenic	65.0%
	GGAGGTTCTCAAGGCTCCGG+TGG	-	chr8.1:24589390-24589409	MS.gene60407:CDS	65.0%
	GGTTCTCAAGGCTCCGGTGG+TGG	-	chr8.1:24589393-24589412	MS.gene60407:CDS	65.0%
!	GTGGTGGGAGGTAGCGTGGT+TGG	-	chr8.1:24589255-24589274	MS.gene60407:CDS	65.0%
!	TCTCAAGGCTCCGGTGGTGG+TGG	-	chr8.1:24589396-24589415	MS.gene60407:CDS	65.0%
!!	ACAGGTGGTGGGAGGTAGCG+TGG	-	chr8.1:24589251-24589270	MS.gene60407:CDS	65.0%
!!	GGACAAGGACAGGTGGTGGG+AGG	-	chr8.1:24589243-24589262	MS.gene60407:CDS	65.0%
	ACAACACCACCAGCCGCCGC+TGG	+	chr8.1:24589123-24589142	None:intergenic	70.0%
	AGCAGCCGGACCAGGAGACG+TGG	-	chr8.1:24588876-24588895	MS.gene60407:CDS	70.0%
	CTGCCACCACCAGCTCCTCC+CGG	-	chr8.1:24589189-24589208	MS.gene60407:CDS	70.0%
	GCCGGACCAGGAGACGTGGT+AGG	-	chr8.1:24588880-24588899	MS.gene60407:CDS	70.0%
	GTTGCTCCGGGAGGAGCTGG+TGG	+	chr8.1:24589198-24589217	None:intergenic	70.0%
!!	CAAGGCTCCGGTGGTGGTGG+TGG	-	chr8.1:24589399-24589418	MS.gene60407:CDS	70.0%
	ATCCGGCAACCAGCGGCGGC+TGG	-	chr8.1:24589111-24589130	MS.gene60407:CDS	75.0%
	CCGGTGGTCTGCCGCGTGGA+CGG	+	chr8.1:24588909-24588928	None:intergenic	75.0%
	CCGTCCACGCGGCAGACCAC+CGG	-	chr8.1:24588906-24588925	MS.gene60407:CDS	75.0%
	CGGCGGCCTACCACGTCTCC+TGG	+	chr8.1:24588889-24588908	None:intergenic	75.0%
	CGTCCACGCGGCAGACCACC+GGG	-	chr8.1:24588907-24588926	MS.gene60407:CDS	75.0%
	GATCCCGGTGGTCTGCCGCG+TGG	+	chr8.1:24588913-24588932	None:intergenic	75.0%
	GCTCCGGGAGGAGCTGGTGG+TGG	+	chr8.1:24589195-24589214	None:intergenic	75.0%
	GTGGTAGGCCGCCGTCCACG+CGG	-	chr8.1:24588895-24588914	MS.gene60407:CDS	75.0%
	GTGGTCTGCCGCGTGGACGG+CGG	+	chr8.1:24588906-24588925	None:intergenic	75.0%
!	CACCAGCCGCCGCTGGTTGC+CGG	+	chr8.1:24589116-24589135	None:intergenic	75.0%
!!	GGCTCCGGTGGTGGTGGTGG+CGG	-	chr8.1:24589402-24589421	MS.gene60407:CDS	75.0%
!	CGGCAACCAGCGGCGGCTGG+TGG	-	chr8.1:24589114-24589133	MS.gene60407:CDS	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	24588679	24589569	24588679	ID=MS.gene60407
chr8.1	mRNA	24588679	24589569	24588679	ID=MS.gene60407.t1;Parent=MS.gene60407
chr8.1	exon	24588679	24589569	24588679	ID=MS.gene60407.t1.exon1;Parent=MS.gene60407.t1
chr8.1	CDS	24588679	24589569	24588679	ID=cds.MS.gene60407.t1;Parent=MS.gene60407.t1

Gene Sequence

>MS.gene60407

ATGGCTGGGTTAGATTTAGGGAGTGCAAGCTTTATGCAAAACATCCACAGGCAAAACTTACACCTTCAACAACAACACCATCACCAAAACCAGCACGATTCTGAAGAACAAGAATCAAATCGTGCCAGTGTTGGTGGTGGTGCACCGTTCTCATCGAACGAAGAAGATGATCGCAGCCAAGGTTTGGAGCTTGGTTCAGCAGCCGGACCAGGAGACGTGGTAGGCCGCCGTCCACGCGGCAGACCACCGGGATCGAAAAACAAAGCCAAACCACCGGTGATCATCACTCGAGAGAGCGCAAACACCCTCAGAGCTCATATTCTTGAAGTTGCCAGTGGTTCTGATGTTTTTGAATGCGTATCCACCTATGCTCGCCGTCGTCAACGTGGGATCTGTGTTCTTAGCGGCAGTGGCACTGTGACAAATGTCAGTATCCGGCAACCAGCGGCGGCTGGTGGTGTTGTTACGCTACATGGAAGATTTGAGATATTGTCCTTGTCTGGATCTTTTCTGCCACCACCAGCTCCTCCCGGAGCAACTAGCTTAACGATTTATCTTGCTGGAGGACAAGGACAGGTGGTGGGAGGTAGCGTGGTTGGTGAGCTTATCGCAGCCGGGCCAGTGATTGTGATTGCAGCTTCTTTTACTAATGTTGCTTATGAGAAGTTGCCGTTGGAAGAGGAGCAGCTTCAGATGCAGGCTGAGGCTGGAGGAGGTTCTCAAGGCTCCGGTGGTGGTGGTGGCGGCAATAACAATAACAATCCATTTCCTGATCCATCTTCTGGTTTACCGTTTTTCAATTTGCCATTGAATATGCAGAATGTTCATCAGTTGCCTGTTGATGGTTGGGCGGGAAACAACAACAACTCAGCTTCACGTCAACCTTTTTGA

Protein sequence

>MS.gene60407.t1

MAGLDLGSASFMQNIHRQNLHLQQQHHHQNQHDSEEQESNRASVGGGAPFSSNEEDDRSQGLELGSAAGPGDVVGRRPRGRPPGSKNKAKPPVIITRESANTLRAHILEVASGSDVFECVSTYARRRQRGICVLSGSGTVTNVSIRQPAAAGGVVTLHGRFEILSLSGSFLPPPAPPGATSLTIYLAGGQGQVVGGSVVGELIAAGPVIVIAASFTNVAYEKLPLEEEQLQMQAEAGGGSQGSGGGGGGNNNNNPFPDPSSGLPFFNLPLNMQNVHQLPVDGWAGNNNNSASRQPF