AlfalfaGEDB | MS.gene60710

M. sativa cultivar XinJiangDaYe / MS.gene60710

KEGG: -
GO: -
PFAM: PF12023.11

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60710.t1	XP_003592063.1	100	86	0	0	1	86	1	86	5.30E-39	169.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60710.t1	G7IBW0	100.0	86	0	0	1	86	1	86	3.8e-39	169.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04918	MS.gene60710	0.803718	3.05E-49	-1.69E-46
MS.gene04920	MS.gene60710	0.80127	9.75E-49	-1.69E-46
MS.gene049254	MS.gene60710	0.807448	5.03E-50	-1.69E-46
MS.gene049350	MS.gene60710	0.828395	9.21E-55	-1.69E-46
MS.gene049711	MS.gene60710	0.80339	3.57E-49	-1.69E-46
MS.gene049807	MS.gene60710	0.801271	9.74E-49	-1.69E-46
MS.gene049825	MS.gene60710	0.814445	1.54E-51	-1.69E-46
MS.gene050300	MS.gene60710	-0.810683	1.02E-50	-1.69E-46
MS.gene050559	MS.gene60710	0.827304	1.68E-54	-1.69E-46
MS.gene050561	MS.gene60710	0.816237	6.13E-52	-1.69E-46
MS.gene050562	MS.gene60710	0.817504	3.19E-52	-1.69E-46
MS.gene050807	MS.gene60710	0.805188	1.51E-49	-1.69E-46
MS.gene051279	MS.gene60710	0.803673	3.12E-49	-1.69E-46
MS.gene051574	MS.gene60710	0.8117	6.14E-51	-1.69E-46
MS.gene051699	MS.gene60710	0.823899	1.08E-53	-1.69E-46
MS.gene051700	MS.gene60710	0.808794	2.60E-50	-1.69E-46
MS.gene051720	MS.gene60710	0.816938	4.27E-52	-1.69E-46
MS.gene052519	MS.gene60710	0.813758	2.18E-51	-1.69E-46
MS.gene052533	MS.gene60710	0.803161	3.98E-49	-1.69E-46
MS.gene052561	MS.gene60710	0.802808	4.71E-49	-1.69E-46
MS.gene052582	MS.gene60710	0.808794	2.60E-50	-1.69E-46
MS.gene052584	MS.gene60710	0.800469	1.42E-48	-1.69E-46
MS.gene052793	MS.gene60710	0.822994	1.76E-53	-1.69E-46
MS.gene053678	MS.gene60710	0.812988	3.21E-51	-1.69E-46
MS.gene053963	MS.gene60710	0.806384	8.45E-50	-1.69E-46
MS.gene054222	MS.gene60710	0.814368	1.60E-51	-1.69E-46
MS.gene054651	MS.gene60710	0.810699	1.01E-50	-1.69E-46
MS.gene055141	MS.gene60710	0.80497	1.67E-49	-1.69E-46
MS.gene055229	MS.gene60710	0.801376	9.27E-49	-1.69E-46
MS.gene055555	MS.gene60710	0.82022	7.70E-53	-1.69E-46
MS.gene056091	MS.gene60710	0.814761	1.31E-51	-1.69E-46
MS.gene056677	MS.gene60710	0.82053	6.53E-53	-1.69E-46
MS.gene057102	MS.gene60710	0.819886	9.18E-53	-1.69E-46
MS.gene057705	MS.gene60710	0.810655	1.03E-50	-1.69E-46
MS.gene058037	MS.gene60710	0.802537	5.35E-49	-1.69E-46
MS.gene058159	MS.gene60710	0.820008	8.61E-53	-1.69E-46
MS.gene058279	MS.gene60710	0.805018	1.63E-49	-1.69E-46
MS.gene058330	MS.gene60710	0.819894	9.14E-53	-1.69E-46
MS.gene05950	MS.gene60710	0.803439	3.48E-49	-1.69E-46
MS.gene05952	MS.gene60710	0.803064	4.17E-49	-1.69E-46
MS.gene05954	MS.gene60710	0.831727	1.42E-55	-1.69E-46
MS.gene05985	MS.gene60710	-0.812197	4.78E-51	-1.69E-46
MS.gene060365	MS.gene60710	0.817664	2.93E-52	-1.69E-46
MS.gene060429	MS.gene60710	0.812528	4.05E-51	-1.69E-46
MS.gene060521	MS.gene60710	0.8111	8.29E-51	-1.69E-46
MS.gene061188	MS.gene60710	0.822727	2.03E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60710.t1	MTR_1g098310	100.000	86	0	0	1	86	1	86	5.20e-57	170
MS.gene60710.t1	MTR_3g104640	60.465	43	17	0	43	85	67	109	1.79e-12	58.2
MS.gene60710.t1	MTR_4g125990	54.386	57	23	1	29	85	69	122	4.44e-12	57.4
MS.gene60710.t1	MTR_2g099630	50.000	48	24	0	38	85	59	106	7.61e-11	53.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60710.t1	AT4G09890	55.914	93	27	4	1	86	2	87	1.23e-24	88.6
MS.gene60710.t1	AT5G11970	54.545	44	20	0	42	85	60	103	6.13e-12	56.6
MS.gene60710.t1	AT3G62640	37.209	86	44	2	10	85	25	110	5.89e-11	54.3
MS.gene60710.t1	AT1G72720	47.170	53	28	0	34	86	74	126	7.75e-11	54.3

Find 16 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTTGTTCTTACACTGAATT+TGG	0.137121	1.2:+71087222	MS.gene60710:CDS
TTCCACTGCCAACACATTAT+AGG	0.253589	1.2:-71087352	None:intergenic
TTCAATGGACCAAGTGATAA+AGG	0.349768	1.2:+71087273	MS.gene60710:CDS
GTGTAAGAACAAGAGTATTC+AGG	0.369295	1.2:-71087213	None:intergenic
TCCATTGAAATTATATGAGT+TGG	0.413854	1.2:-71087259	None:intergenic
CAAATGATTCAGAGCTTAAG+AGG	0.471110	1.2:+71087313	MS.gene60710:CDS
TCCAACTCATATAATTTCAA+TGG	0.471387	1.2:+71087258	MS.gene60710:CDS
AAACCACTTCCTTTATCACT+TGG	0.503804	1.2:-71087282	None:intergenic
AGAACAAGTTCAGCGATGTT+CGG	0.522118	1.2:+71087418	MS.gene60710:CDS
GGACCAAGTGATAAAGGAAG+TGG	0.529951	1.2:+71087279	MS.gene60710:CDS
GTCCTATAATGTGTTGGCAG+TGG	0.532233	1.2:+71087350	MS.gene60710:CDS
TATAATGTGTTGGCAGTGGA+AGG	0.566724	1.2:+71087354	MS.gene60710:CDS
AAGTTCAGCGATGTTCGGTA+TGG	0.569967	1.2:+71087423	MS.gene60710:CDS
GTGTAAGAAACAGCTTCAAG+TGG	0.577880	1.2:+71087391	MS.gene60710:CDS
AATCAAGTCCTATAATGTGT+TGG	0.586946	1.2:+71087344	MS.gene60710:CDS
CTGAATTTGGATACAGTGAA+AGG	0.593305	1.2:+71087235	MS.gene60710:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TCCAACTCATATAATTTCAA+TGG	+	chr1.2:71087258-71087277	MS.gene60710:CDS	25.0%
!	TCCATTGAAATTATATGAGT+TGG	-	chr1.2:71087262-71087281	None:intergenic	25.0%
	AATCAAGTCCTATAATGTGT+TGG	+	chr1.2:71087344-71087363	MS.gene60710:CDS	30.0%
	TCTTGTTCTTACACTGAATT+TGG	+	chr1.2:71087222-71087241	MS.gene60710:CDS	30.0%
	AAACCACTTCCTTTATCACT+TGG	-	chr1.2:71087285-71087304	None:intergenic	35.0%
	CAAATGATTCAGAGCTTAAG+AGG	+	chr1.2:71087313-71087332	MS.gene60710:CDS	35.0%
	CTGAATTTGGATACAGTGAA+AGG	+	chr1.2:71087235-71087254	MS.gene60710:CDS	35.0%
	TTCAATGGACCAAGTGATAA+AGG	+	chr1.2:71087273-71087292	MS.gene60710:CDS	35.0%
!!	GTGTAAGAACAAGAGTATTC+AGG	-	chr1.2:71087216-71087235	None:intergenic	35.0%
	AGAACAAGTTCAGCGATGTT+CGG	+	chr1.2:71087418-71087437	MS.gene60710:CDS	40.0%
	GTGTAAGAAACAGCTTCAAG+TGG	+	chr1.2:71087391-71087410	MS.gene60710:CDS	40.0%
	TTCCACTGCCAACACATTAT+AGG	-	chr1.2:71087355-71087374	None:intergenic	40.0%
!	TATAATGTGTTGGCAGTGGA+AGG	+	chr1.2:71087354-71087373	MS.gene60710:CDS	40.0%
	AAGTTCAGCGATGTTCGGTA+TGG	+	chr1.2:71087423-71087442	MS.gene60710:CDS	45.0%
	GGACCAAGTGATAAAGGAAG+TGG	+	chr1.2:71087279-71087298	MS.gene60710:CDS	45.0%
!	GTCCTATAATGTGTTGGCAG+TGG	+	chr1.2:71087350-71087369	MS.gene60710:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	71087192	71087452	71087192	ID=MS.gene60710
chr1.2	mRNA	71087192	71087452	71087192	ID=MS.gene60710.t1;Parent=MS.gene60710
chr1.2	exon	71087192	71087452	71087192	ID=MS.gene60710.t1.exon1;Parent=MS.gene60710.t1
chr1.2	CDS	71087192	71087452	71087192	ID=cds.MS.gene60710.t1;Parent=MS.gene60710.t1

Gene Sequence	>MS.gene60710 ATGGAGAAAAGCAAGTCATTTCCTGAATACTCTTGTTCTTACACTGAATTTGGATACAGTGAAAGGTCCAACTCATATAATTTCAATGGACCAAGTGATAAAGGAAGTGGTTTTTCTGCATCAAATGATTCAGAGCTTAAGAGGAAGAAGAGAATCAAGTCCTATAATGTGTTGGCAGTGGAAGGAAAGCTTAAAAGTAGTGTAAGAAACAGCTTCAAGTGGATCAAGAACAAGTTCAGCGATGTTCGGTATGGCGTGTGA
Protein sequence	>MS.gene60710.t1 MEKSKSFPEYSCSYTEFGYSERSNSYNFNGPSDKGSGFSASNDSELKRKKRIKSYNVLAVEGKLKSSVRNSFKWIKNKFSDVRYGV