AlfalfaGEDB | MS.gene63469

M. sativa cultivar XinJiangDaYe / MS.gene63469

KEGG: K03364
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF08662.14 PF12894.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63469.t1	XP_024633558.1	99.6	487	1	1	1	487	1	486	1.60E-286	994.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63469.t1	Q8LPL5	74.0	492	112	7	1	487	1	481	1.4e-201	703.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63469.t1	A0A396ISC0	99.6	487	1	1	1	487	1	486	1.2e-286	994.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050875	MS.gene63469	0.823449	1.38E-53	-1.69E-46
MS.gene051816	MS.gene63469	0.810509	1.11E-50	-1.69E-46
MS.gene051896	MS.gene63469	0.83568	1.46E-56	-1.69E-46
MS.gene051914	MS.gene63469	0.865769	4.64E-65	-1.69E-46
MS.gene051955	MS.gene63469	0.803322	3.68E-49	-1.69E-46
MS.gene052308	MS.gene63469	0.887261	1.67E-72	-1.69E-46
MS.gene052367	MS.gene63469	0.807806	4.22E-50	-1.69E-46
MS.gene053173	MS.gene63469	0.85946	4.07E-63	-1.69E-46
MS.gene053348	MS.gene63469	0.848908	4.54E-60	-1.69E-46
MS.gene054551	MS.gene63469	0.870803	1.11E-66	-1.69E-46
MS.gene054552	MS.gene63469	0.82891	6.91E-55	-1.69E-46
MS.gene054593	MS.gene63469	0.87016	1.80E-66	-1.69E-46
MS.gene054633	MS.gene63469	0.857216	1.90E-62	-1.69E-46
MS.gene054662	MS.gene63469	0.803311	3.70E-49	-1.69E-46
MS.gene054850	MS.gene63469	0.804776	1.84E-49	-1.69E-46
MS.gene054941	MS.gene63469	0.805802	1.12E-49	-1.69E-46
MS.gene055308	MS.gene63469	0.861792	8.00E-64	-1.69E-46
MS.gene055578	MS.gene63469	0.854555	1.14E-61	-1.69E-46
MS.gene055579	MS.gene63469	0.817592	3.04E-52	-1.69E-46
MS.gene055957	MS.gene63469	0.819965	8.80E-53	-1.69E-46
MS.gene056041	MS.gene63469	0.861344	1.10E-63	-1.69E-46
MS.gene056042	MS.gene63469	0.813666	2.28E-51	-1.69E-46
MS.gene05640	MS.gene63469	0.874775	5.19E-68	-1.69E-46
MS.gene056526	MS.gene63469	0.807694	4.46E-50	-1.69E-46
MS.gene056528	MS.gene63469	0.873118	1.88E-67	-1.69E-46
MS.gene05652	MS.gene63469	0.857387	1.69E-62	-1.69E-46
MS.gene057101	MS.gene63469	0.801086	1.06E-48	-1.69E-46
MS.gene057458	MS.gene63469	0.832054	1.18E-55	-1.69E-46
MS.gene057669	MS.gene63469	0.800394	1.47E-48	-1.69E-46
MS.gene058013	MS.gene63469	0.826391	2.78E-54	-1.69E-46
MS.gene058128	MS.gene63469	0.899988	1.15E-77	-1.69E-46
MS.gene058789	MS.gene63469	0.802398	5.72E-49	-1.69E-46
MS.gene058853	MS.gene63469	0.866337	3.07E-65	-1.69E-46
MS.gene059553	MS.gene63469	0.80938	1.95E-50	-1.69E-46
MS.gene059616	MS.gene63469	0.855066	8.10E-62	-1.69E-46
MS.gene059905	MS.gene63469	0.801538	8.59E-49	-1.69E-46
MS.gene060473	MS.gene63469	0.834526	2.85E-56	-1.69E-46
MS.gene060565	MS.gene63469	0.822031	2.94E-53	-1.69E-46
MS.gene06093	MS.gene63469	0.856301	3.53E-62	-1.69E-46
MS.gene061099	MS.gene63469	0.885037	1.15E-71	-1.69E-46
MS.gene061260	MS.gene63469	0.806958	6.39E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene013771	MS.gene63469	PPI
MS.gene87756	MS.gene63469	PPI
MS.gene87050	MS.gene63469	PPI
MS.gene03182	MS.gene63469	PPI
MS.gene03180	MS.gene63469	PPI
MS.gene63469	MS.gene42086	PPI
MS.gene86206	MS.gene63469	PPI
MS.gene03219	MS.gene63469	PPI
MS.gene63469	MS.gene62819	PPI
MS.gene63469	MS.gene033401	PPI
MS.gene63469	MS.gene03215	PPI
MS.gene63469	MS.gene02559	PPI
MS.gene44305	MS.gene63469	PPI
MS.gene63469	MS.gene37226	PPI
MS.gene46253	MS.gene63469	PPI
MS.gene048045	MS.gene63469	PPI
MS.gene63469	MS.gene027709	PPI
MS.gene03185	MS.gene63469	PPI
MS.gene63469	MS.gene013775	PPI
MS.gene29182	MS.gene63469	PPI
MS.gene011448	MS.gene63469	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63469.t1	MTR_3g067940	98.152	487	1	2	1	487	1	479	0.0	970
MS.gene63469.t1	MTR_4g102510	62.754	443	152	6	48	487	43	475	0.0	550
MS.gene63469.t1	MTR_3g051690	44.342	433	207	9	57	474	33	446	1.25e-119	358
MS.gene63469.t1	MTR_5g008010	46.099	423	200	8	51	464	26	429	2.49e-119	357
MS.gene63469.t1	MTR_7g106870	51.360	331	147	5	150	466	103	433	4.95e-115	347
MS.gene63469.t1	MTR_7g451830	47.590	332	160	5	155	472	92	423	9.93e-104	317
MS.gene63469.t1	MTR_7g047640	47.590	332	160	5	155	472	92	423	9.93e-104	317
MS.gene63469.t1	MTR_7g053100	47.305	334	160	6	159	476	99	432	1.78e-101	311
MS.gene63469.t1	MTR_7g034625	48.065	310	151	4	167	466	13	322	6.87e-101	306
MS.gene63469.t1	MTR_7g057540	42.824	432	207	10	44	464	3	405	2.66e-100	308
MS.gene63469.t1	MTR_7g055590	42.824	432	207	10	44	464	3	405	4.41e-99	307
MS.gene63469.t1	MTR_2g060710	37.838	444	246	10	57	487	26	452	4.61e-97	300
MS.gene63469.t1	MTR_2g100460	35.833	360	187	7	156	487	43	386	3.62e-63	210
MS.gene63469.t1	MTR_2g100540	36.677	319	163	9	162	472	94	381	2.37e-50	177
MS.gene63469.t1	MTR_7g086360	23.113	212	157	3	256	465	48	255	2.03e-14	74.7
MS.gene63469.t1	MTR_6g453270	37.500	144	36	5	275	418	26	115	1.62e-13	72.4
MS.gene63469.t1	MTR_5g016370	22.945	292	160	9	186	463	312	552	1.77e-13	73.2
MS.gene63469.t1	MTR_1g054300	24.521	261	180	10	204	461	235	481	2.20e-13	72.4
MS.gene63469.t1	MTR_6g004040	26.708	161	112	3	219	377	57	213	9.14e-13	71.2
MS.gene63469.t1	MTR_8g038550	24.719	267	183	10	224	481	186	443	1.49e-12	70.5
MS.gene63469.t1	MTR_4g415070	52.830	53	25	0	412	464	55	107	2.35e-12	68.2
MS.gene63469.t1	MTR_3g106080	25.414	181	128	4	200	378	441	616	1.48e-11	67.4
MS.gene63469.t1	MTR_2g086140	27.854	219	115	8	234	413	24	238	2.38e-11	66.6
MS.gene63469.t1	MTR_4g095058	24.000	250	174	9	224	463	292	535	3.48e-11	65.9
MS.gene63469.t1	MTR_1g072140	25.263	190	134	6	275	462	41	224	4.45e-11	64.3
MS.gene63469.t1	MTR_4g095058	24.000	250	174	9	224	463	290	533	5.02e-11	65.5
MS.gene63469.t1	MTR_2g012630	27.094	203	128	6	189	377	16	212	5.55e-11	65.5
MS.gene63469.t1	MTR_2g012630	27.094	203	128	6	189	377	16	212	5.63e-11	65.9
MS.gene63469.t1	MTR_4g119620	25.991	227	133	9	257	462	21	233	7.01e-11	63.9
MS.gene63469.t1	MTR_4g119620	22.800	250	182	6	219	461	76	321	8.79e-11	63.5
MS.gene63469.t1	MTR_5g016350	24.126	286	164	11	186	463	300	540	8.71e-11	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63469.t1	AT5G13840	79.226	491	88	6	1	487	1	481	0.0	726
MS.gene63469.t1	AT5G13840	79.226	491	88	6	1	487	1	481	0.0	726
MS.gene63469.t1	AT4G22910	62.299	435	149	5	56	487	61	483	0.0	546
MS.gene63469.t1	AT4G11920	62.332	446	147	6	48	487	45	475	0.0	531
MS.gene63469.t1	AT4G33260	43.823	429	206	7	49	464	16	422	2.92e-116	349
MS.gene63469.t1	AT4G33270	43.243	444	206	9	49	472	26	443	1.45e-115	348
MS.gene63469.t1	AT5G27570	51.111	315	145	3	159	464	110	424	2.00e-112	340
MS.gene63469.t1	AT4G33260	42.657	429	211	7	49	464	16	422	1.16e-110	335
MS.gene63469.t1	AT5G27080	49.841	315	149	3	159	464	102	416	8.96e-110	333
MS.gene63469.t1	AT5G26900	48.734	316	154	3	157	464	103	418	2.25e-107	327
MS.gene63469.t1	AT5G27945	45.045	333	173	5	141	464	71	402	2.80e-93	290
MS.gene63469.t1	AT5G13480	25.000	296	203	11	198	482	85	372	3.43e-15	78.6
MS.gene63469.t1	AT5G13480	25.000	296	203	11	198	482	85	372	4.29e-15	78.6
MS.gene63469.t1	AT5G13480	25.000	296	203	11	198	482	97	384	4.71e-15	78.2
MS.gene63469.t1	AT5G13480	25.000	296	203	11	198	482	108	395	4.86e-15	78.2
MS.gene63469.t1	AT5G52820	26.848	257	168	10	211	462	231	472	9.61e-15	77.0
MS.gene63469.t1	AT2G43770	22.170	212	159	3	256	465	46	253	3.04e-14	74.3
MS.gene63469.t1	AT5G23430	27.528	178	122	4	202	377	41	213	3.95e-14	75.5
MS.gene63469.t1	AT5G23430	27.528	178	122	4	202	377	41	213	3.95e-14	75.5
MS.gene63469.t1	AT5G23430	27.528	178	122	4	202	377	41	213	4.46e-14	75.5
MS.gene63469.t1	AT5G08390	26.966	178	123	4	202	377	41	213	5.56e-14	75.1
MS.gene63469.t1	AT3G49660	25.893	224	135	8	257	463	18	227	8.00e-14	72.8
MS.gene63469.t1	AT3G49660	22.581	248	183	5	219	461	69	312	4.01e-12	67.8
MS.gene63469.t1	AT1G61210	26.257	179	123	5	202	377	40	212	1.97e-12	70.5
MS.gene63469.t1	AT1G61210	26.257	179	123	5	202	377	40	212	2.08e-12	70.5
MS.gene63469.t1	AT1G61210	26.257	179	123	5	202	377	40	212	2.08e-12	70.5
MS.gene63469.t1	AT1G11160	27.933	179	120	5	202	377	40	212	2.72e-12	69.7
MS.gene63469.t1	AT1G11160	27.933	179	120	5	202	377	40	212	2.86e-12	69.7
MS.gene63469.t1	AT1G11160	27.933	179	120	5	202	377	40	212	2.91e-12	69.7
MS.gene63469.t1	AT3G18130	27.344	256	156	8	232	463	74	323	3.90e-12	67.8
MS.gene63469.t1	AT1G11160	27.564	156	107	3	224	377	19	170	5.81e-12	68.9
MS.gene63469.t1	AT4G35370	26.230	183	87	6	224	380	219	379	6.05e-12	67.8
MS.gene63469.t1	AT5G25150	24.747	198	142	5	193	388	433	625	8.86e-12	68.2
MS.gene63469.t1	AT3G44530	27.313	227	124	7	224	413	16	238	9.14e-12	68.2
MS.gene63469.t1	AT3G44530	27.313	227	124	7	224	413	16	238	9.44e-12	68.2
MS.gene63469.t1	AT5G25150	24.747	198	142	5	193	388	433	625	1.01e-11	67.8
MS.gene63469.t1	AT1G15440	28.977	176	119	3	296	469	341	512	2.41e-11	66.6
MS.gene63469.t1	AT1G15440	28.977	176	119	3	296	469	381	552	2.63e-11	66.6
MS.gene63469.t1	AT5G43920	25.140	179	125	3	291	463	211	386	3.22e-11	65.9

Find 146 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGCATGGAATTCTCGCATTT+TGG	0.202786	3.1:-56180883	MS.gene63469:CDS
CTTACCTTGTGGGGCGTCTT+AGG	0.222128	3.1:+56182084	None:intergenic
CTGATCGGTGTATTCGTTTC+TGG	0.223974	3.1:-56180388	MS.gene63469:CDS
GGGATGAGACTCTGCGGTTT+TGG	0.238154	3.1:-56179829	MS.gene63469:CDS
TGCTTATGAATCATCTGATT+TGG	0.243659	3.1:+56179645	None:intergenic
CAAAGGCCTGTATCTTTAAC+TGG	0.271405	3.1:+56179683	None:intergenic
CAGAGCAACCTTCTCGTATC+TGG	0.287630	3.1:-56180422	MS.gene63469:CDS
AATTAGGTTGCAACTCTAAC+TGG	0.295863	3.1:-56180028	MS.gene63469:intron
AATATCGAAGGCGAAGAAAA+AGG	0.319187	3.1:+56182168	None:intergenic
TTGGCACTTGTGTTTATCTC+TGG	0.333779	3.1:-56181282	MS.gene63469:CDS
GCTTATGAATCATCTGATTT+GGG	0.337855	3.1:+56179646	None:intergenic
AGGGTTCCAAGCGACTTTAT+TGG	0.361219	3.1:-56180819	MS.gene63469:CDS
TTCATATCCATTGGTACAAA+TGG	0.382848	3.1:-56181086	MS.gene63469:CDS
TCTCCTTCTTCTTCTCCTGC+AGG	0.390074	3.1:-56182270	MS.gene63469:CDS
TCATTACTCCCTTCTTTAAC+CGG	0.390491	3.1:+56182342	None:intergenic
GACTTTATTGGCAAGCTTGT+CGG	0.390886	3.1:-56180807	MS.gene63469:CDS
TCGATATTTGGAAGAAATGA+TGG	0.394918	3.1:-56182153	MS.gene63469:CDS
TACAAATGGTGGTCAAGTTC+AGG	0.401103	3.1:-56181072	MS.gene63469:intron
TCTTCTTCTCCTGCAGGTTG+TGG	0.401970	3.1:-56182264	MS.gene63469:CDS
GGAACTTTCCTAGGAGGCTT+AGG	0.402674	3.1:+56182105	None:intergenic
CCTTCAACTTGACAGGTTTG+TGG	0.403871	3.1:-56180687	MS.gene63469:intron
TCTCCTGCAGGTTGTGGTGT+AGG	0.409021	3.1:-56182258	MS.gene63469:CDS
GATGACCGGGAACTTGCTTC+TGG	0.413514	3.1:-56180648	MS.gene63469:CDS
GTTATTGTTGACATCCCTGC+TGG	0.415431	3.1:+56182537	None:intergenic
CTCTGTTGACACTGGAAGCC+AGG	0.417288	3.1:-56180339	MS.gene63469:intron
TTCTGGTGGTAATGATAATC+AGG	0.417463	3.1:-56180631	MS.gene63469:intron
GCGCGAGGCGAAGCGGAAGA+AGG	0.417481	3.1:+56182483	None:intergenic
TACCACCAGAAGCAAGTTCC+CGG	0.418042	3.1:+56180643	None:intergenic
TGCAGGTGTGTAACCTTGCT+TGG	0.418904	3.1:-56180238	MS.gene63469:intron
TGTGACTTGGGGCCTTATGA+TGG	0.421524	3.1:-56181143	MS.gene63469:CDS
TGCAATGTCTCCTGATGGTC+AGG	0.422711	3.1:-56179981	MS.gene63469:intron
TTTACTTGAATCTTGTGGAT+TGG	0.423069	3.1:-56181333	MS.gene63469:CDS
GGAGTAATGAGGCTTATAAT+AGG	0.434256	3.1:-56182329	MS.gene63469:CDS
CAGTGTCAACAGAGTTCAAT+TGG	0.437042	3.1:+56180348	None:intergenic
CTACTCCTCCTAAGCCTCCT+AGG	0.441271	3.1:-56182113	MS.gene63469:CDS
CGTTTCTGGAACACGACAAA+TGG	0.442087	3.1:-56180374	MS.gene63469:CDS
GGTAACTAAGCTGTGTGACT+TGG	0.442507	3.1:-56181156	MS.gene63469:CDS
ACTCTCAACAACCAACTTTG+AGG	0.442538	3.1:-56180493	MS.gene63469:CDS
TGCAAGAAGGTCAGAACAAT+GGG	0.442773	3.1:-56180933	MS.gene63469:CDS
AAGATAGAAACTTACCTTGT+GGG	0.446408	3.1:+56182074	None:intergenic
GTAACTAAGCTGTGTGACTT+GGG	0.457093	3.1:-56181155	MS.gene63469:CDS
CGCGAGGCGAAGCGGAAGAA+GGG	0.461072	3.1:+56182484	None:intergenic
GATACAGGCCTTTGGTCACT+AGG	0.464727	3.1:-56179674	MS.gene63469:CDS
CAGGCCCCAGTTAAAGATAC+AGG	0.466060	3.1:-56179689	MS.gene63469:CDS
GAAGAAGGAGAAGCAAAATC+AGG	0.474147	3.1:+56182282	None:intergenic
AGGAACCCTCCTTGGTCCAC+TGG	0.476755	3.1:+56181108	None:intergenic
CAGACAATAGTGACTGGTGC+TGG	0.478559	3.1:-56179851	MS.gene63469:CDS
GGAGGGCATCAAACAAGAAC+CGG	0.479441	3.1:-56180912	MS.gene63469:CDS
AATAATACCTTTGCTAGTGA+TGG	0.483310	3.1:+56180146	None:intergenic
TCAAACAAGAACCGGTGTCT+TGG	0.489887	3.1:-56180904	MS.gene63469:CDS
TCCCTTCTTTAACCGGTGAC+TGG	0.494990	3.1:+56182349	None:intergenic
GACAGGTTTGTGGATTGAAA+TGG	0.496996	3.1:-56180677	MS.gene63469:intron
TTGACCACCATTTGTACCAA+TGG	0.497202	3.1:+56181079	None:intergenic
TCTTCAAGACTGCATACATT+CGG	0.497264	3.1:-56182384	MS.gene63469:CDS
AGCAAGTTCCCGGTCATCAC+AGG	0.498721	3.1:+56180653	None:intergenic
TGGACATTTACCTGACCATC+AGG	0.498898	3.1:+56179971	None:intergenic
GGATATGAAGGAACCCTCCT+TGG	0.504932	3.1:+56181100	None:intergenic
TGGCTTCAGGAAGCAGGGAT+AGG	0.505574	3.1:-56180863	MS.gene63469:CDS
CAGTTAAAGATACAGGCCTT+TGG	0.507215	3.1:-56179682	MS.gene63469:CDS
TCAGGTCTAAACCTCCCAGC+AGG	0.508097	3.1:-56182552	MS.gene63469:CDS
GAGGGTTCCTTCATATCCAT+TGG	0.508547	3.1:-56181095	MS.gene63469:CDS
AGCTTGCCAATAAAGTCGCT+TGG	0.510546	3.1:+56180813	None:intergenic
AACCAGTCACCGGTTAAAGA+AGG	0.514252	3.1:-56182351	MS.gene63469:CDS
ATTTGTACCAATGGATATGA+AGG	0.514939	3.1:+56181088	None:intergenic
AACCTCAACATATTCTTACT+CGG	0.518937	3.1:+56182219	None:intergenic
TGAAATGGTCCTGTGATGAC+CGG	0.522032	3.1:-56180662	MS.gene63469:CDS
CGATATTTGGAAGAAATGAT+GGG	0.526164	3.1:-56182152	MS.gene63469:CDS
TATATGAGCAGCTATTGGTA+TGG	0.528902	3.1:-56180523	MS.gene63469:intron
ACTTTCCTAGGAGGCTTAGG+AGG	0.531462	3.1:+56182108	None:intergenic
TGAATCATCTGATTTGGGTG+CGG	0.533378	3.1:+56179651	None:intergenic
TAAAAGTCATCTTGAAGTGA+AGG	0.536272	3.1:+56181352	None:intergenic
CAAGAACCGGTGTCTTGGCA+TGG	0.537570	3.1:-56180899	MS.gene63469:CDS
TATGAATCTGTCACTACACT+TGG	0.538788	3.1:+56182419	None:intergenic
GAATCTGTCACTACACTTGG+AGG	0.541099	3.1:+56182422	None:intergenic
CTGCTGTGAAAGCCATTGCT+TGG	0.542537	3.1:-56180454	MS.gene63469:CDS
ACAGAACAGACACCATCATA+AGG	0.543861	3.1:+56181131	None:intergenic
GGCGTCTTAGGAACTTTCCT+AGG	0.547742	3.1:+56182096	None:intergenic
GAATATGTTGAGGTTTAAGA+CGG	0.551632	3.1:-56182211	MS.gene63469:CDS
TTACTCACAAAATCAAATCA+TGG	0.552909	3.1:-56180180	MS.gene63469:CDS
CAACCTGCAGGAGAAGAAGA+AGG	0.554584	3.1:+56182267	None:intergenic
AGGTTTAAGACGGAGAGTTG+TGG	0.557810	3.1:-56182201	MS.gene63469:CDS
CACAAAATCAAATCATGGTG+TGG	0.560137	3.1:-56180175	MS.gene63469:CDS
TTATTGTTGACATCCCTGCT+GGG	0.562161	3.1:+56182538	None:intergenic
GAAATATCCATCACTAGCAA+AGG	0.565549	3.1:-56180153	MS.gene63469:intron
AATACTCTAGCAGTTGGACT+TGG	0.567179	3.1:-56181301	MS.gene63469:CDS
ATGCAAGAAGGTCAGAACAA+TGG	0.567203	3.1:-56180934	MS.gene63469:CDS
AACCTTCTCGTATCTGGAGG+TGG	0.572049	3.1:-56180416	MS.gene63469:CDS
TCTGTCCAGTGGACCAAGGA+GGG	0.572876	3.1:-56181113	MS.gene63469:CDS
ATGGAGAGGGTGTTGATGAG+AGG	0.580002	3.1:+56182451	None:intergenic
ACCAGTCACCGGTTAAAGAA+GGG	0.584887	3.1:-56182350	MS.gene63469:CDS
TCACAAAATACTCTAGCAGT+TGG	0.586601	3.1:-56181307	MS.gene63469:CDS
ACGAGAAGGTTGCTCTGATG+AGG	0.586636	3.1:+56180428	None:intergenic
ATGGTGTCTGTTCTGTCCAG+TGG	0.586950	3.1:-56181124	MS.gene63469:CDS
AAAGGCCTGTATCTTTAACT+GGG	0.587939	3.1:+56179684	None:intergenic
GAACCTACACCACAACCTGC+AGG	0.589197	3.1:+56182255	None:intergenic
AGACAATAGTGACTGGTGCT+GGG	0.590497	3.1:-56179850	MS.gene63469:CDS
CAATTGAACTCTGTTGACAC+TGG	0.590791	3.1:-56180347	MS.gene63469:CDS
TTGTGGTGTAGGTTCGCCAT+TGG	0.591222	3.1:-56182247	MS.gene63469:CDS
TCATTTCTTCCAAATATCGA+AGG	0.592894	3.1:+56182156	None:intergenic
CTGGAGGTGGAACTGCTGAT+CGG	0.593470	3.1:-56180403	MS.gene63469:CDS
GACCATCAGGAGACATTGCA+AGG	0.595767	3.1:+56179984	None:intergenic
TTCTTACTCGGACTCACCAA+TGG	0.595792	3.1:+56182231	None:intergenic
ATATCCATTGGTACAAATGG+TGG	0.598496	3.1:-56181083	MS.gene63469:CDS
GTCTTAGGAACTTTCCTAGG+AGG	0.599352	3.1:+56182099	None:intergenic
TACCTTGCAATGTCTCCTGA+TGG	0.601989	3.1:-56179986	MS.gene63469:CDS
TGCTCTGTAAGCCTCAAAGT+TGG	0.603299	3.1:+56180482	None:intergenic
TTATGGTAACAGATTTGGGA+TGG	0.603590	3.1:-56180963	MS.gene63469:intron
GCGAGGCGAAGCGGAAGAAG+GGG	0.607095	3.1:+56182485	None:intergenic
GAAATGGTCCTGTGATGACC+GGG	0.607983	3.1:-56180661	MS.gene63469:CDS
TTGATAGCGTTTCTAGACGG+AGG	0.608364	3.1:+56182511	None:intergenic
GTGTTGATAGCGTTTCTAGA+CGG	0.610679	3.1:+56182508	None:intergenic
CTGTTCTGTCCAGTGGACCA+AGG	0.611176	3.1:-56181117	MS.gene63469:CDS
TTCTGTCCAGTGGACCAAGG+AGG	0.616102	3.1:-56181114	MS.gene63469:CDS
CAATTGCAGACAATAGTGAC+TGG	0.618146	3.1:-56179857	MS.gene63469:intron
TGAGATCGCGCGAGGCGAAG+CGG	0.618949	3.1:+56182476	None:intergenic
GGATGGTACTAAATGCAAGA+AGG	0.620237	3.1:-56180946	MS.gene63469:CDS
TAACTAAGCTGTGTGACTTG+GGG	0.621200	3.1:-56181154	MS.gene63469:CDS
CTGATGAGGTGACCAAGCAA+TGG	0.623695	3.1:+56180442	None:intergenic
AGATAGAAACTTACCTTGTG+GGG	0.626078	3.1:+56182075	None:intergenic
AGCAACCTTCTCGTATCTGG+AGG	0.630408	3.1:-56180419	MS.gene63469:CDS
TGATAGCGTTTCTAGACGGA+GGG	0.630489	3.1:+56182512	None:intergenic
TAGCGTTTCTAGACGGAGGG+AGG	0.631148	3.1:+56182515	None:intergenic
GGGTGCGGCCTAGTGACCAA+AGG	0.631232	3.1:+56179666	None:intergenic
TGTCAACAGAGTTCAATTGG+TGG	0.632166	3.1:+56180351	None:intergenic
CAGGTCTAAACCTCCCAGCA+GGG	0.636073	3.1:-56182551	MS.gene63469:CDS
ACATACTTCAGCATGACATG+AGG	0.639225	3.1:-56180839	MS.gene63469:CDS
TTGTTGACATCCCTGCTGGG+AGG	0.640070	3.1:+56182541	None:intergenic
TTCCACCTCCAGATACGAGA+AGG	0.640711	3.1:+56180414	None:intergenic
AGAATTCCATGCCAAGACAC+CGG	0.640925	3.1:+56180893	None:intergenic
ATTAATCGATAACCAGTCAC+CGG	0.643856	3.1:-56182361	MS.gene63469:CDS
AGTTCCTAAGACGCCCCACA+AGG	0.646840	3.1:-56182088	MS.gene63469:intron
AAGAAGGTCAGAACAATGGG+AGG	0.649698	3.1:-56180930	MS.gene63469:CDS
CGAGGCGAAGCGGAAGAAGG+GGG	0.655782	3.1:+56182486	None:intergenic
GACAATAGTGACTGGTGCTG+GGG	0.659564	3.1:-56179849	MS.gene63469:CDS
CATACTTCAGCATGACATGA+GGG	0.660026	3.1:-56180838	MS.gene63469:CDS
AGAAGGTCAGAACAATGGGA+GGG	0.662939	3.1:-56180929	MS.gene63469:CDS
GAGAGGTTTGAGATCGCGCG+AGG	0.662959	3.1:+56182468	None:intergenic
GGTTAAAGAAGGGAGTAATG+AGG	0.665035	3.1:-56182340	MS.gene63469:CDS
GACCGGGAACTTGCTTCTGG+TGG	0.665213	3.1:-56180645	MS.gene63469:CDS
GTCCGAGTAAGAATATGTTG+AGG	0.666814	3.1:-56182221	MS.gene63469:CDS
AATGAGCTAGTGAGTACACA+TGG	0.668243	3.1:-56180203	MS.gene63469:CDS
GGTATAGTACTCTCATGCTG+TGG	0.672992	3.1:+56180005	None:intergenic
GCTTGTCGGCCACAAATCCG+AGG	0.677734	3.1:-56180793	MS.gene63469:intron
AGTCTTGAAGATGATCTGCA+AGG	0.682253	3.1:+56182396	None:intergenic
AAGGCCTGTATCTTTAACTG+GGG	0.685695	3.1:+56179685	None:intergenic
GTGCTGGGGATGAGACTCTG+CGG	0.730545	3.1:-56179835	MS.gene63469:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATTGATGTAAAAATTAA+GGG	+	chr3.1:56182335-56182354	None:intergenic	10.0%
!!	TAAAATTGATGTAAAAATTA+AGG	+	chr3.1:56182336-56182355	None:intergenic	10.0%
!!	TAATTAAGAAAAATTTCATA+TGG	+	chr3.1:56182188-56182207	None:intergenic	10.0%
!!	TTGTTTATGATATAATAATA+GGG	+	chr3.1:56182301-56182320	None:intergenic	10.0%
!!	TTTGTTTATGATATAATAAT+AGG	+	chr3.1:56182302-56182321	None:intergenic	10.0%
!!!	ATATGAAATTTTTCTTAATT+AGG	-	chr3.1:56182187-56182206	MS.gene63469:CDS	10.0%
!!	TCAAAAAATTTACACAAAAA+CGG	+	chr3.1:56180194-56180213	None:intergenic	15.0%
!!!	ATTTTATTGTATTTGAGAAT+TGG	-	chr3.1:56180229-56180248	MS.gene63469:CDS	15.0%
!!!	TTTTATTGTATTTGAGAATT+GGG	-	chr3.1:56180230-56180249	MS.gene63469:CDS	15.0%
!!	AATATGCAATAATTGGAAAT+TGG	+	chr3.1:56182473-56182492	None:intergenic	20.0%
!!	AGTTACAATCATTAATATTG+CGG	-	chr3.1:56180528-56180547	MS.gene63469:intron	20.0%
!!	TATGTAAGTTATGAAATTGA+TGG	-	chr3.1:56181511-56181530	MS.gene63469:intron	20.0%
!!!	ACAAATTAATTGTGATTTTG+AGG	-	chr3.1:56180445-56180464	MS.gene63469:CDS	20.0%
!!!	ACTTGATAATCATTTCTAAT+TGG	+	chr3.1:56182145-56182164	None:intergenic	20.0%
!!!	ATATTGATTCTTCAAAAAAG+AGG	+	chr3.1:56181930-56181949	None:intergenic	20.0%
!!!	ATTAGGATTTTCTACAATTA+GGG	+	chr3.1:56180652-56180671	None:intergenic	20.0%
!!!	CACAATTAATTTGTAAAACT+AGG	+	chr3.1:56180439-56180458	None:intergenic	20.0%
!!!	TATTTAATGCATAATTTTGC+AGG	-	chr3.1:56181976-56181995	MS.gene63469:intron	20.0%
!	AACATAAGAAACTGTTATGT+TGG	-	chr3.1:56180610-56180629	MS.gene63469:intron	25.0%
!	AATTGTAGAAAATCCTAATC+TGG	-	chr3.1:56180653-56180672	MS.gene63469:CDS	25.0%
!	ATACTAAACAGATAGATAGA+AGG	+	chr3.1:56181649-56181668	None:intergenic	25.0%
!	ATTGAACATATATCTTTGTG+TGG	-	chr3.1:56181046-56181065	MS.gene63469:intron	25.0%
!	CAAAGTTTAAGTATTTGAGA+GGG	-	chr3.1:56180754-56180773	MS.gene63469:intron	25.0%
!	GGAACATAATATGCAATAAT+TGG	+	chr3.1:56182480-56182499	None:intergenic	25.0%
!	TAATCAAATCCTACAAATCA+AGG	+	chr3.1:56180271-56180290	None:intergenic	25.0%
!	TTACTCACAAAATCAAATCA+TGG	-	chr3.1:56182051-56182070	MS.gene63469:intron	25.0%
!!	GATTAGGATTTTCTACAATT+AGG	+	chr3.1:56180653-56180672	None:intergenic	25.0%
!!	GTTAAATGGTTTTTATACCT+CGG	+	chr3.1:56181458-56181477	None:intergenic	25.0%
!!	TTGTTGAAATCTGAACTTTT+TGG	-	chr3.1:56179925-56179944	MS.gene63469:intron	25.0%
!!!	AACTCTTTTAAATCTAGAGT+TGG	+	chr3.1:56180706-56180725	None:intergenic	25.0%
!!!	ACAATTAGGGTTTTAAATAG+AGG	+	chr3.1:56180639-56180658	None:intergenic	25.0%
!!!	TTGATTTGTAGGATTTGATT+AGG	-	chr3.1:56180270-56180289	MS.gene63469:intron	25.0%
	AAAACTAGGATTGTCATGAT+TGG	+	chr3.1:56180425-56180444	None:intergenic	30.0%
	AAAGATAGAAACTTACCTTG+TGG	+	chr3.1:56180161-56180180	None:intergenic	30.0%
	AACCTCAACATATTCTTACT+CGG	+	chr3.1:56180015-56180034	None:intergenic	30.0%
	AAGATAGAAACTTACCTTGT+GGG	+	chr3.1:56180160-56180179	None:intergenic	30.0%
	AATAATACCTTTGCTAGTGA+TGG	+	chr3.1:56182088-56182107	None:intergenic	30.0%
	AATCAACTCATAACCAGATT+AGG	+	chr3.1:56180669-56180688	None:intergenic	30.0%
	ATTTGTACCAATGGATATGA+AGG	+	chr3.1:56181146-56181165	None:intergenic	30.0%
	CAAATCAAAGTAAGCTAACA+AGG	+	chr3.1:56181187-56181206	None:intergenic	30.0%
	CAACCATCAAATAAAACACA+TGG	+	chr3.1:56181220-56181239	None:intergenic	30.0%
	CCAAAGTTTAAGTATTTGAG+AGG	-	chr3.1:56180753-56180772	MS.gene63469:intron	30.0%
	CCTCTCAAATACTTAAACTT+TGG	+	chr3.1:56180756-56180775	None:intergenic	30.0%
	CGATATTTGGAAGAAATGAT+GGG	-	chr3.1:56180079-56180098	MS.gene63469:intron	30.0%
	CTGAAGCAAAAAGAAAAGTT+AGG	+	chr3.1:56182525-56182544	None:intergenic	30.0%
	GAATATGTTGAGGTTTAAGA+CGG	-	chr3.1:56180020-56180039	MS.gene63469:CDS	30.0%
	TAAAAGTCATCTTGAAGTGA+AGG	+	chr3.1:56180882-56180901	None:intergenic	30.0%
	TAGGGAATTATGTTGTTATG+TGG	+	chr3.1:56182283-56182302	None:intergenic	30.0%
	TATGCTATGACTCTATCTTA+GGG	+	chr3.1:56180360-56180379	None:intergenic	30.0%
	TCATTTCTTCCAAATATCGA+AGG	+	chr3.1:56180078-56180097	None:intergenic	30.0%
	TCGATATTTGGAAGAAATGA+TGG	-	chr3.1:56180078-56180097	MS.gene63469:intron	30.0%
	TGATAAGATGCTTTCTTGTT+TGG	+	chr3.1:56181953-56181972	None:intergenic	30.0%
	TTCATATCCATTGGTACAAA+TGG	-	chr3.1:56181145-56181164	MS.gene63469:CDS	30.0%
	TTTACTTGAATCTTGTGGAT+TGG	-	chr3.1:56180898-56180917	MS.gene63469:CDS	30.0%
!	CTAACTTTTCTTTTTGCTTC+AGG	-	chr3.1:56182523-56182542	MS.gene63469:CDS	30.0%
!	CTTGTTAGCTTACTTTGATT+TGG	-	chr3.1:56181185-56181204	MS.gene63469:intron	30.0%
!	GAGAATTGGGTTAGATAATA+TGG	-	chr3.1:56180243-56180262	MS.gene63469:intron	30.0%
!	GAGTTCTTTTAAGCAATACT+AGG	+	chr3.1:56182501-56182520	None:intergenic	30.0%
!	TATATCTTTGTGTGGTTTTC+AGG	-	chr3.1:56181054-56181073	MS.gene63469:intron	30.0%
!	TGACTTTTACTTGAATCTTG+TGG	-	chr3.1:56180893-56180912	MS.gene63469:CDS	30.0%
!	TGTTTTATATGAGCAGCTAT+TGG	-	chr3.1:56181703-56181722	MS.gene63469:intron	30.0%
!	TTACAGGTGTTTTCATTGAT+GGG	+	chr3.1:56182436-56182455	None:intergenic	30.0%
!	TTACATTTTTACTCCAAGCA+AGG	+	chr3.1:56182009-56182028	None:intergenic	30.0%
!	TTTTTATCCTTCAACTTGAC+AGG	-	chr3.1:56181537-56181556	MS.gene63469:intron	30.0%
!!	AATATGGTACCTTGATTTGT+AGG	-	chr3.1:56180259-56180278	MS.gene63469:intron	30.0%
!!!	AAAGTGAGTATCGATTTTGT+AGG	-	chr3.1:56180296-56180315	MS.gene63469:intron	30.0%
!!!	AAATGATGGGTTTTGTAATG+AGG	-	chr3.1:56180092-56180111	MS.gene63469:intron	30.0%
!!!	AATGATGGGTTTTGTAATGA+GGG	-	chr3.1:56180093-56180112	MS.gene63469:intron	30.0%
!!!	CATCCATGTGTTTTATTTGA+TGG	-	chr3.1:56181214-56181233	MS.gene63469:intron	30.0%
	AAAGGCCTGTATCTTTAACT+GGG	+	chr3.1:56182550-56182569	None:intergenic	35.0%
	AAGAGGTAAGAAAGTAAACC+TGG	+	chr3.1:56181913-56181932	None:intergenic	35.0%
	AATATCGAAGGCGAAGAAAA+AGG	+	chr3.1:56180066-56180085	None:intergenic	35.0%
	AATGATTGTAACTGTTACCG+CGG	+	chr3.1:56180521-56180540	None:intergenic	35.0%
	AGATAGAAACTTACCTTGTG+GGG	+	chr3.1:56180159-56180178	None:intergenic	35.0%
	ATATCCATTGGTACAAATGG+TGG	-	chr3.1:56181148-56181167	MS.gene63469:CDS	35.0%
	ATCCCAAATCTGTTACCATA+AGG	+	chr3.1:56181269-56181288	None:intergenic	35.0%
	ATGACTCTATCTTAGGGTTT+AGG	+	chr3.1:56180354-56180373	None:intergenic	35.0%
	ATGATTATGAACTTCCCAAG+TGG	-	chr3.1:56180996-56181015	MS.gene63469:intron	35.0%
	ATTAATCGATAACCAGTCAC+CGG	-	chr3.1:56179870-56179889	MS.gene63469:intron	35.0%
	CACAAAATCAAATCATGGTG+TGG	-	chr3.1:56182056-56182075	MS.gene63469:intron	35.0%
	CTATGCTATGACTCTATCTT+AGG	+	chr3.1:56180361-56180380	None:intergenic	35.0%
	CTCAATGCGTATCTGTTAAA+TGG	+	chr3.1:56181472-56181491	None:intergenic	35.0%
	GAAATATCCATCACTAGCAA+AGG	-	chr3.1:56182078-56182097	MS.gene63469:intron	35.0%
	GGAGTAATGAGGCTTATAAT+AGG	-	chr3.1:56179902-56179921	MS.gene63469:intron	35.0%
	GTCATTACAACGTAAGACTA+GGG	-	chr3.1:56180482-56180501	MS.gene63469:CDS	35.0%
	TATATGAGCAGCTATTGGTA+TGG	-	chr3.1:56181708-56181727	MS.gene63469:intron	35.0%
	TATGAATCTGTCACTACACT+TGG	+	chr3.1:56179815-56179834	None:intergenic	35.0%
	TCACAAAATACTCTAGCAGT+TGG	-	chr3.1:56180924-56180943	MS.gene63469:CDS	35.0%
	TCACCACAAGCAAAAAAATC+AGG	-	chr3.1:56179661-56179680	MS.gene63469:CDS	35.0%
	TCATTACTCCCTTCTTTAAC+CGG	+	chr3.1:56179892-56179911	None:intergenic	35.0%
	TCTTCAAGACTGCATACATT+CGG	-	chr3.1:56179847-56179866	MS.gene63469:CDS	35.0%
	TGCCTTATGGTAACAGATTT+GGG	-	chr3.1:56181264-56181283	MS.gene63469:CDS	35.0%
	TGTCATTACAACGTAAGACT+AGG	-	chr3.1:56180481-56180500	MS.gene63469:CDS	35.0%
	TTATGGTAACAGATTTGGGA+TGG	-	chr3.1:56181268-56181287	MS.gene63469:CDS	35.0%
	TTGCCTTATGGTAACAGATT+TGG	-	chr3.1:56181263-56181282	MS.gene63469:intron	35.0%
!	AATTAGGTTGCAACTCTAAC+TGG	-	chr3.1:56182203-56182222	MS.gene63469:CDS	35.0%
!	CTTACAGGTGTTTTCATTGA+TGG	+	chr3.1:56182437-56182456	None:intergenic	35.0%
!	GTTTTTATACCTCGGATTTG+TGG	+	chr3.1:56181450-56181469	None:intergenic	35.0%
!	TTCTGGTGGTAATGATAATC+AGG	-	chr3.1:56181600-56181619	MS.gene63469:intron	35.0%
!	TTGTGATATTGTTGCCACTT+GGG	+	chr3.1:56181013-56181032	None:intergenic	35.0%
!	TTTTCTTCGCCTTCGATATT+TGG	-	chr3.1:56180066-56180085	MS.gene63469:intron	35.0%
!!	AACTGCTAGAGTATTTTGTG+AGG	+	chr3.1:56180925-56180944	None:intergenic	35.0%
!!!	AAGACTAGGGTTTTAAATGG+AGG	-	chr3.1:56180495-56180514	MS.gene63469:CDS	35.0%
!!!	AAGAGAGCTTTTTTGCCTTA+TGG	-	chr3.1:56181251-56181270	MS.gene63469:intron	35.0%
!!!	AGACCTGATTTTTTTGCTTG+TGG	+	chr3.1:56179667-56179686	None:intergenic	35.0%
!!!	CGTAAGACTAGGGTTTTAAA+TGG	-	chr3.1:56180492-56180511	MS.gene63469:CDS	35.0%
	AAGGCCTGTATCTTTAACTG+GGG	+	chr3.1:56182549-56182568	None:intergenic	40.0%
	AATACTCTAGCAGTTGGACT+TGG	-	chr3.1:56180930-56180949	MS.gene63469:CDS	40.0%
	AATGAGCTAGTGAGTACACA+TGG	-	chr3.1:56182028-56182047	MS.gene63469:intron	40.0%
	ACAGAACAGACACCATCATA+AGG	+	chr3.1:56181103-56181122	None:intergenic	40.0%
	ACATACTTCAGCATGACATG+AGG	-	chr3.1:56181392-56181411	MS.gene63469:intron	40.0%
	ACTCTCAACAACCAACTTTG+AGG	-	chr3.1:56181738-56181757	MS.gene63469:intron	40.0%
	AGTCTTGAAGATGATCTGCA+AGG	+	chr3.1:56179838-56179857	None:intergenic	40.0%
	AGTGAGTGAGACAGTGTATA+AGG	-	chr3.1:56180386-56180405	MS.gene63469:CDS	40.0%
	ATGCAAGAAGGTCAGAACAA+TGG	-	chr3.1:56181297-56181316	MS.gene63469:CDS	40.0%
	CAAAGGCCTGTATCTTTAAC+TGG	+	chr3.1:56182551-56182570	None:intergenic	40.0%
	CAATTGAACTCTGTTGACAC+TGG	-	chr3.1:56181884-56181903	MS.gene63469:intron	40.0%
	CAATTGCAGACAATAGTGAC+TGG	-	chr3.1:56182374-56182393	MS.gene63469:CDS	40.0%
	CAGTGTCAACAGAGTTCAAT+TGG	+	chr3.1:56181886-56181905	None:intergenic	40.0%
	CAGTTAAAGATACAGGCCTT+TGG	-	chr3.1:56182549-56182568	MS.gene63469:CDS	40.0%
	CATACTTCAGCATGACATGA+GGG	-	chr3.1:56181393-56181412	MS.gene63469:intron	40.0%
	GAAGAAGGAGAAGCAAAATC+AGG	+	chr3.1:56179952-56179971	None:intergenic	40.0%
	GACTTTATTGGCAAGCTTGT+CGG	-	chr3.1:56181424-56181443	MS.gene63469:intron	40.0%
	GGATGGTACTAAATGCAAGA+AGG	-	chr3.1:56181285-56181304	MS.gene63469:CDS	40.0%
	GGTTAAAGAAGGGAGTAATG+AGG	-	chr3.1:56179891-56179910	MS.gene63469:intron	40.0%
	GTAACTAAGCTGTGTGACTT+GGG	-	chr3.1:56181076-56181095	MS.gene63469:CDS	40.0%
	GTCCGAGTAAGAATATGTTG+AGG	-	chr3.1:56180010-56180029	MS.gene63469:CDS	40.0%
	GTTGTGATATTGTTGCCACT+TGG	+	chr3.1:56181014-56181033	None:intergenic	40.0%
	TAACTAAGCTGTGTGACTTG+GGG	-	chr3.1:56181077-56181096	MS.gene63469:CDS	40.0%
	TACAAATGGTGGTCAAGTTC+AGG	-	chr3.1:56181159-56181178	MS.gene63469:intron	40.0%
	TGCAAGAAGGTCAGAACAAT+GGG	-	chr3.1:56181298-56181317	MS.gene63469:CDS	40.0%
	TGTCAACAGAGTTCAATTGG+TGG	+	chr3.1:56181883-56181902	None:intergenic	40.0%
	TTATTGTTGACATCCCTGCT+GGG	+	chr3.1:56179696-56179715	None:intergenic	40.0%
	TTGACCACCATTTGTACCAA+TGG	+	chr3.1:56181155-56181174	None:intergenic	40.0%
	TTGGCACTTGTGTTTATCTC+TGG	-	chr3.1:56180949-56180968	MS.gene63469:CDS	40.0%
!	CTACACTTGGAGGATTTTGA+TGG	+	chr3.1:56179802-56179821	None:intergenic	40.0%
!	GACAGGTTTGTGGATTGAAA+TGG	-	chr3.1:56181554-56181573	MS.gene63469:intron	40.0%
!!	GTGTTGATAGCGTTTCTAGA+CGG	+	chr3.1:56179726-56179745	None:intergenic	40.0%
!!	TTGGAGGATTTTGATGGAGA+GGG	+	chr3.1:56179796-56179815	None:intergenic	40.0%
!!!	AATTCTCGCATTTTGGCTTC+AGG	-	chr3.1:56181355-56181374	MS.gene63469:CDS	40.0%
	AAGAAGGTCAGAACAATGGG+AGG	-	chr3.1:56181301-56181320	MS.gene63469:CDS	45.0%
	AAGGAAGCAATTGTGTCTGC+TGG	+	chr3.1:56181630-56181649	None:intergenic	45.0%
	AATTGAGCAATGCTGTGTGC+AGG	-	chr3.1:56180845-56180864	MS.gene63469:CDS	45.0%
	AGAAGGTCAGAACAATGGGA+GGG	-	chr3.1:56181302-56181321	MS.gene63469:CDS	45.0%
	AGAATTCCATGCCAAGACAC+CGG	+	chr3.1:56181341-56181360	None:intergenic	45.0%
	AGACAATAGTGACTGGTGCT+GGG	-	chr3.1:56182381-56182400	MS.gene63469:CDS	45.0%
	AGCTTGCCAATAAAGTCGCT+TGG	+	chr3.1:56181421-56181440	None:intergenic	45.0%
	AGGGTTCCAAGCGACTTTAT+TGG	-	chr3.1:56181412-56181431	MS.gene63469:intron	45.0%
	CCTTCAACTTGACAGGTTTG+TGG	-	chr3.1:56181544-56181563	MS.gene63469:intron	45.0%
	CTGATCGGTGTATTCGTTTC+TGG	-	chr3.1:56181843-56181862	MS.gene63469:intron	45.0%
	GAATCTGTCACTACACTTGG+AGG	+	chr3.1:56179812-56179831	None:intergenic	45.0%
	GAGGGTTCCTTCATATCCAT+TGG	-	chr3.1:56181136-56181155	MS.gene63469:CDS	45.0%
	GGTAACTAAGCTGTGTGACT+TGG	-	chr3.1:56181075-56181094	MS.gene63469:CDS	45.0%
	GGTATAGTACTCTCATGCTG+TGG	+	chr3.1:56182229-56182248	None:intergenic	45.0%
	GTCTTAGGAACTTTCCTAGG+AGG	+	chr3.1:56180135-56180154	None:intergenic	45.0%
	GTTATTGTTGACATCCCTGC+TGG	+	chr3.1:56179697-56179716	None:intergenic	45.0%
	TACCTTGCAATGTCTCCTGA+TGG	-	chr3.1:56182245-56182264	MS.gene63469:CDS	45.0%
	TCAAACAAGAACCGGTGTCT+TGG	-	chr3.1:56181327-56181346	MS.gene63469:CDS	45.0%
	TGAAATGGTCCTGTGATGAC+CGG	-	chr3.1:56181569-56181588	MS.gene63469:intron	45.0%
	TGATGCGACGTGAAAATCAG+TGG	-	chr3.1:56180572-56180591	MS.gene63469:intron	45.0%
	TGCTCTGTAAGCCTCAAAGT+TGG	+	chr3.1:56181752-56181771	None:intergenic	45.0%
	TGGACATTTACCTGACCATC+AGG	+	chr3.1:56182263-56182282	None:intergenic	45.0%
	TTCTTACTCGGACTCACCAA+TGG	+	chr3.1:56180003-56180022	None:intergenic	45.0%
!	AACCAGTCACCGGTTAAAGA+AGG	-	chr3.1:56179880-56179899	MS.gene63469:intron	45.0%
!	ACCAGTCACCGGTTAAAGAA+GGG	-	chr3.1:56179881-56179900	MS.gene63469:intron	45.0%
!	AGGTTTAAGACGGAGAGTTG+TGG	-	chr3.1:56180030-56180049	MS.gene63469:intron	45.0%
!	CACCTGTAAGCATTGCATTG+TGG	-	chr3.1:56182447-56182466	MS.gene63469:CDS	45.0%
!	GGCATGGAATTCTCGCATTT+TGG	-	chr3.1:56181348-56181367	MS.gene63469:CDS	45.0%
!	TGATAGCGTTTCTAGACGGA+GGG	+	chr3.1:56179722-56179741	None:intergenic	45.0%
!!	CCACAAACCTGTCAAGTTGA+AGG	+	chr3.1:56181547-56181566	None:intergenic	45.0%
!!	CGTTTCTGGAACACGACAAA+TGG	-	chr3.1:56181857-56181876	MS.gene63469:intron	45.0%
!!	CTTGGAGGATTTTGATGGAG+AGG	+	chr3.1:56179797-56179816	None:intergenic	45.0%
!!	TTGATAGCGTTTCTAGACGG+AGG	+	chr3.1:56179723-56179742	None:intergenic	45.0%
!!!	CATTTTGGCTTCAGGAAGCA+GGG	-	chr3.1:56181363-56181382	MS.gene63469:CDS	45.0%
	AACCTTCTCGTATCTGGAGG+TGG	-	chr3.1:56181815-56181834	MS.gene63469:intron	50.0%
	ACGAGAAGGTTGCTCTGATG+AGG	+	chr3.1:56181806-56181825	None:intergenic	50.0%
	ACTTTCCTAGGAGGCTTAGG+AGG	+	chr3.1:56180126-56180145	None:intergenic	50.0%
	AGCAACCTTCTCGTATCTGG+AGG	-	chr3.1:56181812-56181831	MS.gene63469:intron	50.0%
	ATGGTGTCTGTTCTGTCCAG+TGG	-	chr3.1:56181107-56181126	MS.gene63469:CDS	50.0%
	CAACCTGCAGGAGAAGAAGA+AGG	+	chr3.1:56179967-56179986	None:intergenic	50.0%
	CAGACAATAGTGACTGGTGC+TGG	-	chr3.1:56182380-56182399	MS.gene63469:CDS	50.0%
	CAGAGCAACCTTCTCGTATC+TGG	-	chr3.1:56181809-56181828	MS.gene63469:intron	50.0%
	CAGGCCCCAGTTAAAGATAC+AGG	-	chr3.1:56182542-56182561	MS.gene63469:CDS	50.0%
	CTGATGAGGTGACCAAGCAA+TGG	+	chr3.1:56181792-56181811	None:intergenic	50.0%
	CTGCTGTGAAAGCCATTGCT+TGG	-	chr3.1:56181777-56181796	MS.gene63469:intron	50.0%
	GAAATGGTCCTGTGATGACC+GGG	-	chr3.1:56181570-56181589	MS.gene63469:intron	50.0%
	GACCATCAGGAGACATTGCA+AGG	+	chr3.1:56182250-56182269	None:intergenic	50.0%
	GATACAGGCCTTTGGTCACT+AGG	-	chr3.1:56182557-56182576	MS.gene63469:CDS	50.0%
	GGAACTTTCCTAGGAGGCTT+AGG	+	chr3.1:56180129-56180148	None:intergenic	50.0%
	GGATATGAAGGAACCCTCCT+TGG	+	chr3.1:56181134-56181153	None:intergenic	50.0%
	GGCCACAATGCAATGCTTAC+AGG	+	chr3.1:56182452-56182471	None:intergenic	50.0%
	GGCGAAGAAAAAGGCGAAGA+AGG	+	chr3.1:56180057-56180076	None:intergenic	50.0%
	GGCGTCTTAGGAACTTTCCT+AGG	+	chr3.1:56180138-56180157	None:intergenic	50.0%
	TACCACCAGAAGCAAGTTCC+CGG	+	chr3.1:56181591-56181610	None:intergenic	50.0%
	TCCCTTCTTTAACCGGTGAC+TGG	+	chr3.1:56179885-56179904	None:intergenic	50.0%
	TCTCCTTCTTCTTCTCCTGC+AGG	-	chr3.1:56179961-56179980	MS.gene63469:intron	50.0%
	TCTTCTTCTCCTGCAGGTTG+TGG	-	chr3.1:56179967-56179986	MS.gene63469:intron	50.0%
	TGCAATGTCTCCTGATGGTC+AGG	-	chr3.1:56182250-56182269	MS.gene63469:CDS	50.0%
	TGCAGGTGTGTAACCTTGCT+TGG	-	chr3.1:56181993-56182012	MS.gene63469:intron	50.0%
	TTCCACCTCCAGATACGAGA+AGG	+	chr3.1:56181820-56181839	None:intergenic	50.0%
	TTGTGGTGTAGGTTCGCCAT+TGG	-	chr3.1:56179984-56180003	MS.gene63469:CDS	50.0%
!	GACAATAGTGACTGGTGCTG+GGG	-	chr3.1:56182382-56182401	MS.gene63469:CDS	50.0%
!	TGTGACTTGGGGCCTTATGA+TGG	-	chr3.1:56181088-56181107	MS.gene63469:CDS	50.0%
!!	ATGGAGAGGGTGTTGATGAG+AGG	+	chr3.1:56179783-56179802	None:intergenic	50.0%
!!	GGAGGGCATCAAACAAGAAC+CGG	-	chr3.1:56181319-56181338	MS.gene63469:CDS	50.0%
!!!	AGGGTTTTAAATGGAGGCCG+CGG	-	chr3.1:56180501-56180520	MS.gene63469:CDS	50.0%
!!!	GCATTTTGGCTTCAGGAAGC+AGG	-	chr3.1:56181362-56181381	MS.gene63469:CDS	50.0%
	AGCAAGTTCCCGGTCATCAC+AGG	+	chr3.1:56181581-56181600	None:intergenic	55.0%
	AGTTCCTAAGACGCCCCACA+AGG	-	chr3.1:56180143-56180162	MS.gene63469:intron	55.0%
	CAAGAACCGGTGTCTTGGCA+TGG	-	chr3.1:56181332-56181351	MS.gene63469:CDS	55.0%
	CAGGTCTAAACCTCCCAGCA+GGG	-	chr3.1:56179680-56179699	MS.gene63469:CDS	55.0%
	CTACTCCTCCTAAGCCTCCT+AGG	-	chr3.1:56180118-56180137	MS.gene63469:intron	55.0%
	CTCTGTTGACACTGGAAGCC+AGG	-	chr3.1:56181892-56181911	MS.gene63469:intron	55.0%
	CTGGAGGTGGAACTGCTGAT+CGG	-	chr3.1:56181828-56181847	MS.gene63469:intron	55.0%
	CTGTTCTGTCCAGTGGACCA+AGG	-	chr3.1:56181114-56181133	MS.gene63469:CDS	55.0%
	GAACCTACACCACAACCTGC+AGG	+	chr3.1:56179979-56179998	None:intergenic	55.0%
	GCAATGCTGTGTGCAGGTTC+TGG	-	chr3.1:56180851-56180870	MS.gene63469:CDS	55.0%
	TCAGGTCTAAACCTCCCAGC+AGG	-	chr3.1:56179679-56179698	MS.gene63469:CDS	55.0%
	TCTCCTGCAGGTTGTGGTGT+AGG	-	chr3.1:56179973-56179992	MS.gene63469:intron	55.0%
	TCTGTCCAGTGGACCAAGGA+GGG	-	chr3.1:56181118-56181137	MS.gene63469:CDS	55.0%
	TGGCTTCAGGAAGCAGGGAT+AGG	-	chr3.1:56181368-56181387	MS.gene63469:intron	55.0%
	TTCTGTCCAGTGGACCAAGG+AGG	-	chr3.1:56181117-56181136	MS.gene63469:CDS	55.0%
	TTGTTGACATCCCTGCTGGG+AGG	+	chr3.1:56179693-56179712	None:intergenic	55.0%
!	CTTACCTTGTGGGGCGTCTT+AGG	+	chr3.1:56180150-56180169	None:intergenic	55.0%
!	GATGACCGGGAACTTGCTTC+TGG	-	chr3.1:56181583-56181602	MS.gene63469:intron	55.0%
!	GGGATGAGACTCTGCGGTTT+TGG	-	chr3.1:56182402-56182421	MS.gene63469:CDS	55.0%
!	TAGCGTTTCTAGACGGAGGG+AGG	+	chr3.1:56179719-56179738	None:intergenic	55.0%
	AGGAACCCTCCTTGGTCCAC+TGG	+	chr3.1:56181126-56181145	None:intergenic	60.0%
	GCTTGTCGGCCACAAATCCG+AGG	-	chr3.1:56181438-56181457	MS.gene63469:intron	60.0%
	GTGCTGGGGATGAGACTCTG+CGG	-	chr3.1:56182396-56182415	MS.gene63469:CDS	60.0%
!	GACCGGGAACTTGCTTCTGG+TGG	-	chr3.1:56181586-56181605	MS.gene63469:intron	60.0%
!	GAGAGGTTTGAGATCGCGCG+AGG	+	chr3.1:56179766-56179785	None:intergenic	60.0%
	CGAGGCGAAGCGGAAGAAGG+GGG	+	chr3.1:56179748-56179767	None:intergenic	65.0%
	CGCGAGGCGAAGCGGAAGAA+GGG	+	chr3.1:56179750-56179769	None:intergenic	65.0%
	GCGAGGCGAAGCGGAAGAAG+GGG	+	chr3.1:56179749-56179768	None:intergenic	65.0%
	GGGTGCGGCCTAGTGACCAA+AGG	+	chr3.1:56182568-56182587	None:intergenic	65.0%
	TGAGATCGCGCGAGGCGAAG+CGG	+	chr3.1:56179758-56179777	None:intergenic	65.0%
	GCGCGAGGCGAAGCGGAAGA+AGG	+	chr3.1:56179751-56179770	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	56179655	56182598	56179655	ID=MS.gene63469
chr3.1	mRNA	56179655	56182598	56179655	ID=MS.gene63469.t1;Parent=MS.gene63469
chr3.1	exon	56182089	56182598	56182089	ID=MS.gene63469.t1.exon1;Parent=MS.gene63469.t1
chr3.1	CDS	56182089	56182598	56182089	ID=cds.MS.gene63469.t1;Parent=MS.gene63469.t1
chr3.1	exon	56181264	56181386	56181264	ID=MS.gene63469.t1.exon2;Parent=MS.gene63469.t1
chr3.1	CDS	56181264	56181386	56181264	ID=cds.MS.gene63469.t1;Parent=MS.gene63469.t1
chr3.1	exon	56181073	56181177	56181073	ID=MS.gene63469.t1.exon3;Parent=MS.gene63469.t1
chr3.1	CDS	56181073	56181177	56181073	ID=cds.MS.gene63469.t1;Parent=MS.gene63469.t1
chr3.1	exon	56180794	56180973	56180794	ID=MS.gene63469.t1.exon4;Parent=MS.gene63469.t1
chr3.1	CDS	56180794	56180973	56180794	ID=cds.MS.gene63469.t1;Parent=MS.gene63469.t1
chr3.1	exon	56180632	56180694	56180632	ID=MS.gene63469.t1.exon5;Parent=MS.gene63469.t1
chr3.1	CDS	56180632	56180694	56180632	ID=cds.MS.gene63469.t1;Parent=MS.gene63469.t1
chr3.1	exon	56180340	56180534	56180340	ID=MS.gene63469.t1.exon6;Parent=MS.gene63469.t1
chr3.1	CDS	56180340	56180534	56180340	ID=cds.MS.gene63469.t1;Parent=MS.gene63469.t1
chr3.1	exon	56180154	56180255	56180154	ID=MS.gene63469.t1.exon7;Parent=MS.gene63469.t1
chr3.1	CDS	56180154	56180255	56180154	ID=cds.MS.gene63469.t1;Parent=MS.gene63469.t1
chr3.1	exon	56179982	56180044	56179982	ID=MS.gene63469.t1.exon8;Parent=MS.gene63469.t1
chr3.1	CDS	56179982	56180044	56179982	ID=cds.MS.gene63469.t1;Parent=MS.gene63469.t1
chr3.1	exon	56179802	56179870	56179802	ID=MS.gene63469.t1.exon9;Parent=MS.gene63469.t1
chr3.1	CDS	56179802	56179870	56179802	ID=cds.MS.gene63469.t1;Parent=MS.gene63469.t1
chr3.1	exon	56179655	56179708	56179655	ID=MS.gene63469.t1.exon10;Parent=MS.gene63469.t1
chr3.1	CDS	56179655	56179708	56179655	ID=cds.MS.gene63469.t1;Parent=MS.gene63469.t1

Gene Sequence

>MS.gene63469

ATGGAGTCACCACAAGCAAAAAAATCAGGTCTAAACCTCCCAGCAGGGATGTCAACAATAACCTCCCTCCGTCTAGAAACGCTATCAACACCCCCTTCTTCCGCTTCGCCTCGCGCGATCTCAAACCTCTCATCAACACCCTCTCCATCAAAATCCTCCAAGTGTAGTGACAGATTCATACCTTGCAGATCATCTTCAAGACTGCATACATTCGGATTAATCGATAACCAGTCACCGGTTAAAGAAGGGAGTAATGAGGCTTATAATAGGTTGTTGAAATCTGAACTTTTTGGACCTGATTTTGCTTCTCCTTCTTCTTCTCCTGCAGGTTGTGGTGTAGGTTCGCCATTGGTGAGTCCGAGTAAGAATATGTTGAGGTTTAAGACGGAGAGTTGTGGACCTTCTTCGCCTTTTTCTTCGCCTTCGATATTTGGAAGAAATGATGGGTTTTGTAATGAGGGTTCTACTCCTCCTAAGCCTCCTAGGAAAGTTCCTAAGACGCCCCACAAGGTAAGTTTCTATCTTTTTCTCTTTTACCGTTTTTGTGTAAATTTTTTGAACATGAAATTGTTTTATTTTATTGTATTTGAGAATTGGGTTAGATAATATGGTACCTTGATTTGTAGGATTTGATTAGGTCTAAAGTGAGTATCGATTTTGTAGGATATAGTTAGTTAGTTACACTTACAAGTTACACCTAAACCCTAAGATAGAGTCATAGCATAGCTCTTAGTGAGTGAGACAGTGTATAAGGAAATGCTCAATTTCCAATCATGACAATCCTAGTTTTACAAATTAATTGTGATTTTGAGGTAGACTCGAGCTGTGTCATTACAACGTAAGACTAGGGTTTTAAATGGAGGCCGCGGTAACAGTTACAATCATTAATATTGCGGAAAATTGTAGTCAAATGCAGCTGATGCGACGTGAAAATCAGTGGCATTGCAATTTCGAAAACATAAGAAACTGTTATGTTGGCAACCTCTATTTAAAACCCTAATTGTAGAAAATCCTAATCTGGTTATGAGTTGATTAAAGTTGAGTTTTTCCAACTCTAGATTTAAAAGAGTTTGTGCATGTACATTTCAAGTTCTAAAACCAAAGTTTAAGTATTTGAGAGGGTCTGTCTTTGATACAATAGACAAAAAATTCTTAGAGCTAATAATGATTGTTGAATGTTGATTTTCTAAAATTGAGCAATGCTGTGTGCAGGTTCTGGATGCTCCTTCACTTCAAGATGACTTTTACTTGAATCTTGTGGATTGGTCCTCACAAAATACTCTAGCAGTTGGACTTGGCACTTGTGTTTATCTCTGGAGTGCTTCAAACAGCAAAGTGAGTATGATTATGAACTTCCCAAGTGGCAACAATATCACAACATGTACAATCAAATTGAACATATATCTTTGTGTGGTTTTCAGGTAACTAAGCTGTGTGACTTGGGGCCTTATGATGGTGTCTGTTCTGTCCAGTGGACCAAGGAGGGTTCCTTCATATCCATTGGTACAAATGGTGGTCAAGTTCAGGTACCTTGTTAGCTTACTTTGATTTGGTTTTATCATCCATGTGTTTTATTTGATGGTTGAGAATAACAATAAGAGAGCTTTTTTGCCTTATGGTAACAGATTTGGGATGGTACTAAATGCAAGAAGGTCAGAACAATGGGAGGGCATCAAACAAGAACCGGTGTCTTGGCATGGAATTCTCGCATTTTGGCTTCAGGAAGCAGGGATAGGAACATACTTCAGCATGACATGAGGGTTCCAAGCGACTTTATTGGCAAGCTTGTCGGCCACAAATCCGAGGTATAAAAACCATTTAACAGATACGCATTGAGCTAGTCATAAGATCAATTGTATGTAAGTTATGAAATTGATGGTAATTTTTATCCTTCAACTTGACAGGTTTGTGGATTGAAATGGTCCTGTGATGACCGGGAACTTGCTTCTGGTGGTAATGATAATCAGGTATGCCAGCAGACACAATTGCTTCCTTCTATCTATCTGTTTAGTATTTTTCGTGAGATTTAATTCATCAATTCGATGTCATGTTTTATATGAGCAGCTATTGGTATGGAATCAGCACTCTCAACAACCAACTTTGAGGCTTACAGAGCACACTGCTGCTGTGAAAGCCATTGCTTGGTCACCTCATCAGAGCAACCTTCTCGTATCTGGAGGTGGAACTGCTGATCGGTGTATTCGTTTCTGGAACACGACAAATGGCCACCAATTGAACTCTGTTGACACTGGAAGCCAGGTTTACTTTCTTACCTCTTTTTTGAAGAATCAATATCCAAACAAGAAAGCATCTTATCAAACTATTTAATGCATAATTTTGCAGGTGTGTAACCTTGCTTGGAGTAAAAATGTAAATGAGCTAGTGAGTACACATGGTTACTCACAAAATCAAATCATGGTGTGGAAATATCCATCACTAGCAAAGGTATTATTTTACATTGAGAATTCTAGATATTTTAGACATATTCCAATTAGAAATGATTATCAAGTATAAAATTAAAATGTCTTAACCATATGAAATTTTTCTTAATTAGGTTGCAACTCTAACTGGCCACAGCATGAGAGTACTATACCTTGCAATGTCTCCTGATGGTCAGGTAAATGTCCACATAACAACATAATTCCCTATTATTATATCATAAACAAAAACAAATTGTCCCTTAATTTTTACATCAATTTTAATGTAATGCTGAATAATACAATTGCAGACAATAGTGACTGGTGCTGGGGATGAGACTCTGCGGTTTTGGAATGTTTTCCCATCAATGAAAACACCTGTAAGCATTGCATTGTGGCCAATTTCCAATTATTGCATATTATGTTCCTAGTATTGCTTAAAAGAACTCTCCTAACTTTTCTTTTTGCTTCAGGCCCCAGTTAAAGATACAGGCCTTTGGTCACTAGGCCGCACCCAAATCAGATGA

Protein sequence

>MS.gene63469.t1

MESPQAKKSGLNLPAGMSTITSLRLETLSTPPSSASPRAISNLSSTPSPSKSSKCSDRFIPCRSSSRLHTFGLIDNQSPVKEGSNEAYNRLLKSELFGPDFASPSSSPAGCGVGSPLVSPSKNMLRFKTESCGPSSPFSSPSIFGRNDGFCNEGSTPPKPPRKVPKTPHKVLDAPSLQDDFYLNLVDWSSQNTLAVGLGTCVYLWSASNSKVTKLCDLGPYDGVCSVQWTKEGSFISIGTNGGQVQIWDGTKCKKVRTMGGHQTRTGVLAWNSRILASGSRDRNILQHDMRVPSDFIGKLVGHKSEVCGLKWSCDDRELASGGNDNQLLVWNQHSQQPTLRLTEHTAAVKAIAWSPHQSNLLVSGGGTADRCIRFWNTTNGHQLNSVDTGSQVCNLAWSKNVNELVSTHGYSQNQIMVWKYPSLAKVATLTGHSMRVLYLAMSPDGQTIVTGAGDETLRFWNVFPSMKTPAPVKDTGLWSLGRTQIR