Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64572.t1 | XP_013448823.1 | 95 | 120 | 6 | 0 | 1 | 120 | 1 | 120 | 6.00E-57 | 229.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64572.t1 | P81529 | 36.6 | 112 | 70 | 1 | 1 | 112 | 1 | 111 | 1.0e-12 | 74.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64572.t1 | A0A072UAN8 | 95.0 | 120 | 6 | 0 | 1 | 120 | 1 | 120 | 4.3e-57 | 229.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049222 | MS.gene64572 | 0.815069 | 1.12E-51 | -1.69E-46 |
| MS.gene050504 | MS.gene64572 | 0.808745 | 2.66E-50 | -1.69E-46 |
| MS.gene050577 | MS.gene64572 | 0.800109 | 1.68E-48 | -1.69E-46 |
| MS.gene050868 | MS.gene64572 | 0.827434 | 1.57E-54 | -1.69E-46 |
| MS.gene050871 | MS.gene64572 | 0.828171 | 1.04E-54 | -1.69E-46 |
| MS.gene051838 | MS.gene64572 | 0.807076 | 6.03E-50 | -1.69E-46 |
| MS.gene052164 | MS.gene64572 | 0.823099 | 1.66E-53 | -1.69E-46 |
| MS.gene052503 | MS.gene64572 | 0.806596 | 7.62E-50 | -1.69E-46 |
| MS.gene052823 | MS.gene64572 | 0.808098 | 3.66E-50 | -1.69E-46 |
| MS.gene052825 | MS.gene64572 | 0.807645 | 4.57E-50 | -1.69E-46 |
| MS.gene052872 | MS.gene64572 | 0.800685 | 1.28E-48 | -1.69E-46 |
| MS.gene053480 | MS.gene64572 | 0.821871 | 3.21E-53 | -1.69E-46 |
| MS.gene053632 | MS.gene64572 | 0.827958 | 1.17E-54 | -1.69E-46 |
| MS.gene053824 | MS.gene64572 | 0.843933 | 1.03E-58 | -1.69E-46 |
| MS.gene054600 | MS.gene64572 | 0.81736 | 3.43E-52 | -1.69E-46 |
| MS.gene05470 | MS.gene64572 | 0.821582 | 3.74E-53 | -1.69E-46 |
| MS.gene05494 | MS.gene64572 | 0.821357 | 4.21E-53 | -1.69E-46 |
| MS.gene055362 | MS.gene64572 | 0.800693 | 1.28E-48 | -1.69E-46 |
| MS.gene055577 | MS.gene64572 | 0.838264 | 3.19E-57 | -1.69E-46 |
| MS.gene05589 | MS.gene64572 | 0.805415 | 1.35E-49 | -1.69E-46 |
| MS.gene055941 | MS.gene64572 | 0.827383 | 1.61E-54 | -1.69E-46 |
| MS.gene056387 | MS.gene64572 | 0.819186 | 1.33E-52 | -1.69E-46 |
| MS.gene056449 | MS.gene64572 | 0.804754 | 1.86E-49 | -1.69E-46 |
| MS.gene056504 | MS.gene64572 | 0.823292 | 1.50E-53 | -1.69E-46 |
| MS.gene056947 | MS.gene64572 | 0.818796 | 1.63E-52 | -1.69E-46 |
| MS.gene057048 | MS.gene64572 | 0.819695 | 1.01E-52 | -1.69E-46 |
| MS.gene057658 | MS.gene64572 | 0.806442 | 8.21E-50 | -1.69E-46 |
| MS.gene057857 | MS.gene64572 | 0.817414 | 3.34E-52 | -1.69E-46 |
| MS.gene05787 | MS.gene64572 | 0.806364 | 8.53E-50 | -1.69E-46 |
| MS.gene057926 | MS.gene64572 | 0.807551 | 4.78E-50 | -1.69E-46 |
| MS.gene05837 | MS.gene64572 | 0.800386 | 1.48E-48 | -1.69E-46 |
| MS.gene058723 | MS.gene64572 | 0.808411 | 3.14E-50 | -1.69E-46 |
| MS.gene059023 | MS.gene64572 | 0.82636 | 2.83E-54 | -1.69E-46 |
| MS.gene05913 | MS.gene64572 | 0.802687 | 4.98E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene64572 | 0.802391 | 5.74E-49 | -1.69E-46 |
| MS.gene059718 | MS.gene64572 | 0.805789 | 1.13E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene64572 | 0.800317 | 1.53E-48 | -1.69E-46 |
| MS.gene06000 | MS.gene64572 | 0.806211 | 9.18E-50 | -1.69E-46 |
| MS.gene060703 | MS.gene64572 | 0.801861 | 7.37E-49 | -1.69E-46 |
| MS.gene060762 | MS.gene64572 | 0.814737 | 1.32E-51 | -1.69E-46 |
| MS.gene060778 | MS.gene64572 | 0.81874 | 1.67E-52 | -1.69E-46 |
| MS.gene06077 | MS.gene64572 | 0.81236 | 4.41E-51 | -1.69E-46 |
| MS.gene060982 | MS.gene64572 | 0.802288 | 6.02E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64572.t1 | MTR_7g059460 | 95.000 | 120 | 6 | 0 | 1 | 120 | 1 | 120 | 4.94e-80 | 231 |
| MS.gene64572.t1 | MTR_7g059460 | 95.192 | 104 | 5 | 0 | 1 | 104 | 1 | 104 | 2.00e-67 | 199 |
| MS.gene64572.t1 | MTR_4g014040 | 64.286 | 112 | 40 | 0 | 1 | 112 | 1 | 112 | 6.93e-49 | 152 |
| MS.gene64572.t1 | MTR_4g014040 | 62.617 | 107 | 40 | 0 | 1 | 107 | 1 | 107 | 1.51e-45 | 144 |
| MS.gene64572.t1 | MTR_4g014040 | 63.810 | 105 | 38 | 0 | 1 | 105 | 1 | 105 | 6.42e-44 | 140 |
| MS.gene64572.t1 | MTR_4g127370 | 50.893 | 112 | 55 | 0 | 1 | 112 | 739 | 850 | 5.44e-31 | 115 |
| MS.gene64572.t1 | MTR_4g127360 | 48.214 | 112 | 58 | 0 | 1 | 112 | 1 | 112 | 1.14e-30 | 105 |
| MS.gene64572.t1 | MTR_4g013730 | 44.660 | 103 | 57 | 0 | 1 | 103 | 1 | 103 | 8.74e-30 | 103 |
| MS.gene64572.t1 | MTR_0562s0020 | 44.660 | 103 | 57 | 0 | 1 | 103 | 1 | 103 | 8.74e-30 | 103 |
| MS.gene64572.t1 | MTR_7g070250 | 40.566 | 106 | 63 | 0 | 1 | 106 | 1 | 106 | 1.31e-28 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64572.t1 | AT5G01650 | 60.870 | 115 | 45 | 0 | 1 | 115 | 1 | 115 | 2.01e-47 | 148 |
| MS.gene64572.t1 | AT5G01650 | 62.617 | 107 | 40 | 0 | 1 | 107 | 1 | 107 | 1.58e-45 | 143 |
| MS.gene64572.t1 | AT5G01650 | 61.682 | 107 | 41 | 0 | 1 | 107 | 1 | 107 | 7.16e-45 | 142 |
| MS.gene64572.t1 | AT5G01650 | 61.682 | 107 | 41 | 0 | 1 | 107 | 1 | 107 | 7.16e-45 | 142 |
| MS.gene64572.t1 | AT5G57170 | 47.321 | 112 | 59 | 0 | 1 | 112 | 1 | 112 | 7.17e-30 | 103 |
| MS.gene64572.t1 | AT5G57170 | 48.544 | 103 | 53 | 0 | 1 | 103 | 1 | 103 | 4.98e-29 | 102 |
| MS.gene64572.t1 | AT3G51660 | 39.216 | 102 | 62 | 0 | 1 | 102 | 1 | 102 | 3.51e-23 | 87.0 |
Find 29 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGTGTTCATACAGATTT+GGG | 0.230111 | 7.3:+44822532 | None:intergenic |
| AGAGTTGAGTTGAGAGAGTA+TGG | 0.230816 | 7.3:+44822579 | None:intergenic |
| CTTAGTTTGTTGTTCAAATC+TGG | 0.254690 | 7.3:+44821727 | None:intergenic |
| TCAACAAACGTTAGCTTTGA+TGG | 0.285904 | 7.3:-44822616 | MS.gene64572:CDS |
| GAAAAGTGTTCATACAGATT+TGG | 0.288874 | 7.3:+44822531 | None:intergenic |
| ACAAGTTTCTCGGAATCATT+TGG | 0.359454 | 7.3:+44821470 | None:intergenic |
| GTTATAATTGTGGACTGAAT+GGG | 0.367727 | 7.3:-44821512 | MS.gene64572:CDS |
| GGATCAATACCAATATGTTT+TGG | 0.376407 | 7.3:-44821805 | MS.gene64572:CDS |
| TCAGTTTCACCAAAACATAT+TGG | 0.384156 | 7.3:+44821796 | None:intergenic |
| GGTTATAATTGTGGACTGAA+TGG | 0.389437 | 7.3:-44821513 | MS.gene64572:CDS |
| TATGGTGAATTTGTGGCTAT+TGG | 0.409602 | 7.3:-44821760 | MS.gene64572:CDS |
| TATGTGACGGTTTCATTAGA+AGG | 0.443254 | 7.3:-44821826 | MS.gene64572:CDS |
| AGTTCTTCACACCATGTTGT+CGG | 0.443341 | 7.3:-44821689 | MS.gene64572:CDS |
| AAAGCTAACGTTTGTTGAAA+GGG | 0.447172 | 7.3:+44822621 | None:intergenic |
| ACTGAGGAGCCAGCAGCATA+TGG | 0.461454 | 7.3:-44821778 | MS.gene64572:CDS |
| CAAAGCTAACGTTTGTTGAA+AGG | 0.463062 | 7.3:+44822620 | None:intergenic |
| AATTTGTAGGGTTATAATTG+TGG | 0.473862 | 7.3:-44821522 | MS.gene64572:intron |
| AGCAGCATATGGTGAATTTG+TGG | 0.510208 | 7.3:-44821767 | MS.gene64572:CDS |
| ACAAATTCACCATATGCTGC+TGG | 0.542569 | 7.3:+44821769 | None:intergenic |
| TTATAATTGTGGACTGAATG+GGG | 0.546367 | 7.3:-44821511 | MS.gene64572:CDS |
| CAACATGGTGTGAAGAACTA+AGG | 0.556695 | 7.3:+44821693 | None:intergenic |
| TTTAAGTAGAAACGTGACTT+GGG | 0.574926 | 7.3:+44821664 | None:intergenic |
| TTTGGGGTAACACAAGATGT+TGG | 0.578477 | 7.3:+44822549 | None:intergenic |
| TGACTTGGGCACCGACAACA+TGG | 0.611246 | 7.3:+44821678 | None:intergenic |
| TATAATTGTGGACTGAATGG+GGG | 0.631220 | 7.3:-44821510 | MS.gene64572:CDS |
| TTTGTTGAAAGGGTGAGACA+AGG | 0.631716 | 7.3:+44822631 | None:intergenic |
| TATTGATTTGCAGTATGTGA+CGG | 0.638900 | 7.3:-44821839 | MS.gene64572:intron |
| CTTAAAATTCTATGACACTG+AGG | 0.702342 | 7.3:-44821647 | MS.gene64572:intron |
| AAAGTGTTCATACAGATTTG+GGG | 0.721663 | 7.3:+44822533 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAAATTATTCATTAGTTTTA+TGG | - | chr7.3:44821652-44821671 | MS.gene64572:CDS | 10.0% |
| !! | ACAAATATTAGTAACTAGAT+AGG | - | chr7.3:44821759-44821778 | MS.gene64572:CDS | 20.0% |
| !! | CAATTCAAAATAGTAAATGT+TGG | + | chr7.3:44822500-44822519 | None:intergenic | 20.0% |
| !! | CTAACAAAAATGTTTCAATA+AGG | + | chr7.3:44821842-44821861 | None:intergenic | 20.0% |
| !! | TAGTTATCGAATATTTAATC+CGG | - | chr7.3:44822038-44822057 | MS.gene64572:intron | 20.0% |
| !!! | AAATTTAAAGACGAATCTTT+TGG | - | chr7.3:44821798-44821817 | MS.gene64572:CDS | 20.0% |
| !!! | CATATGTTTGTTAATTTGTA+GGG | - | chr7.3:44822585-44822604 | MS.gene64572:CDS | 20.0% |
| !!! | CTTTAAAATGAGATAAAAAC+CGG | + | chr7.3:44822060-44822079 | None:intergenic | 20.0% |
| !!! | TCATATGTTTGTTAATTTGT+AGG | - | chr7.3:44822584-44822603 | MS.gene64572:CDS | 20.0% |
| !!! | TTTTAGTTATTTTTCCTTCA+TGG | - | chr7.3:44821952-44821971 | MS.gene64572:intron | 20.0% |
| ! | AATTTGTAGGGTTATAATTG+TGG | - | chr7.3:44822597-44822616 | MS.gene64572:CDS | 25.0% |
| ! | AGACCTAAAACAAAATACAA+GGG | - | chr7.3:44822113-44822132 | MS.gene64572:intron | 25.0% |
| ! | CAAATGAGTTAAAAGAACTA+TGG | + | chr7.3:44821987-44822006 | None:intergenic | 25.0% |
| !!! | AAAAGTGTTCATACAGATTT+GGG | + | chr7.3:44821590-44821609 | None:intergenic | 25.0% |
| !!! | AATCCCTTGTATTTTGTTTT+AGG | + | chr7.3:44822119-44822138 | None:intergenic | 25.0% |
| !!! | GTTATAGTCTTGTGAAAATA+TGG | - | chr7.3:44821679-44821698 | MS.gene64572:CDS | 25.0% |
| ATACATTCATGCAAATAGAG+AGG | + | chr7.3:44821629-44821648 | None:intergenic | 30.0% | |
| CTTAAAATTCTATGACACTG+AGG | - | chr7.3:44822472-44822491 | MS.gene64572:intron | 30.0% | |
| CTTAACCTTCCAATTCTTTA+AGG | + | chr7.3:44821917-44821936 | None:intergenic | 30.0% | |
| GAGACCTAAAACAAAATACA+AGG | - | chr7.3:44822112-44822131 | MS.gene64572:intron | 30.0% | |
| TATGGTTCAAATTTCCATGA+AGG | + | chr7.3:44821969-44821988 | None:intergenic | 30.0% | |
| TCAAGAATAGTAAATGAGAG+TGG | + | chr7.3:44822545-44822564 | None:intergenic | 30.0% | |
| TCAGTTTCACCAAAACATAT+TGG | + | chr7.3:44822326-44822345 | None:intergenic | 30.0% | |
| TTTAAGTAGAAACGTGACTT+GGG | + | chr7.3:44822458-44822477 | None:intergenic | 30.0% | |
| TTTAGGTCTCTTAATCTGTT+GGG | + | chr7.3:44822102-44822121 | None:intergenic | 30.0% | |
| ! | AAAGCTAACGTTTGTTGAAA+GGG | + | chr7.3:44821501-44821520 | None:intergenic | 30.0% |
| ! | AAAGTGTTCATACAGATTTG+GGG | + | chr7.3:44821589-44821608 | None:intergenic | 30.0% |
| ! | GGATCAATACCAATATGTTT+TGG | - | chr7.3:44822314-44822333 | MS.gene64572:intron | 30.0% |
| ! | GTTATAATTGTGGACTGAAT+GGG | - | chr7.3:44822607-44822626 | MS.gene64572:CDS | 30.0% |
| ! | TTATAATTGTGGACTGAATG+GGG | - | chr7.3:44822608-44822627 | MS.gene64572:CDS | 30.0% |
| !! | CTTAGTTTGTTGTTCAAATC+TGG | + | chr7.3:44822395-44822414 | None:intergenic | 30.0% |
| !! | GAAAAGTGTTCATACAGATT+TGG | + | chr7.3:44821591-44821610 | None:intergenic | 30.0% |
| !! | TATTGATTTGCAGTATGTGA+CGG | - | chr7.3:44822280-44822299 | MS.gene64572:intron | 30.0% |
| !! | TTTTAAGTAGAAACGTGACT+TGG | + | chr7.3:44822459-44822478 | None:intergenic | 30.0% |
| !! | TTTTAGGTCTCTTAATCTGT+TGG | + | chr7.3:44822103-44822122 | None:intergenic | 30.0% |
| !!! | AATATGTTTTGGTGAAACTG+AGG | - | chr7.3:44822325-44822344 | MS.gene64572:intron | 30.0% |
| !!! | TCTCATTTTAAAGTAATCCC+TGG | - | chr7.3:44822067-44822086 | MS.gene64572:intron | 30.0% |
| ATGCTAAGCTAGAATTCAAG+TGG | + | chr7.3:44821885-44821904 | None:intergenic | 35.0% | |
| GATTCCCTTCCTTAAAGAAT+TGG | - | chr7.3:44821905-44821924 | MS.gene64572:intron | 35.0% | |
| TATGGTGAATTTGTGGCTAT+TGG | - | chr7.3:44822359-44822378 | MS.gene64572:intron | 35.0% | |
| TATGTGACGGTTTCATTAGA+AGG | - | chr7.3:44822293-44822312 | MS.gene64572:intron | 35.0% | |
| ! | ACCTTCCAATTCTTTAAGGA+AGG | + | chr7.3:44821913-44821932 | None:intergenic | 35.0% |
| ! | CAAAGCTAACGTTTGTTGAA+AGG | + | chr7.3:44821502-44821521 | None:intergenic | 35.0% |
| ! | CCTTCCAATTCTTTAAGGAA+GGG | + | chr7.3:44821912-44821931 | None:intergenic | 35.0% |
| ! | GGTTATAATTGTGGACTGAA+TGG | - | chr7.3:44822606-44822625 | MS.gene64572:CDS | 35.0% |
| ! | TATAATTGTGGACTGAATGG+GGG | - | chr7.3:44822609-44822628 | MS.gene64572:CDS | 35.0% |
| !! | TCAACAAACGTTAGCTTTGA+TGG | - | chr7.3:44821503-44821522 | MS.gene64572:CDS | 35.0% |
| !!! | ATTTTAAAGTAATCCCTGGC+AGG | - | chr7.3:44822071-44822090 | MS.gene64572:intron | 35.0% |
| !!! | TTTTAAAGTAATCCCTGGCA+GGG | - | chr7.3:44822072-44822091 | MS.gene64572:intron | 35.0% |
| ACAAATTCACCATATGCTGC+TGG | + | chr7.3:44822353-44822372 | None:intergenic | 40.0% | |
| AGCAGCATATGGTGAATTTG+TGG | - | chr7.3:44822352-44822371 | MS.gene64572:intron | 40.0% | |
| AGTTCTTCACACCATGTTGT+CGG | - | chr7.3:44822430-44822449 | MS.gene64572:intron | 40.0% | |
| CAACATGGTGTGAAGAACTA+AGG | + | chr7.3:44822429-44822448 | None:intergenic | 40.0% | |
| CCCTTCCTTAAAGAATTGGA+AGG | - | chr7.3:44821909-44821928 | MS.gene64572:intron | 40.0% | |
| TTTGTTGAAAGGGTGAGACA+AGG | + | chr7.3:44821491-44821510 | None:intergenic | 40.0% | |
| ! | TTTGGGGTAACACAAGATGT+TGG | + | chr7.3:44821573-44821592 | None:intergenic | 40.0% |
| !! | AGAGTTGAGTTGAGAGAGTA+TGG | + | chr7.3:44821543-44821562 | None:intergenic | 40.0% |
| AGAGAGGCTTAAGAGAGAGA+AGG | + | chr7.3:44821613-44821632 | None:intergenic | 45.0% | |
| GGTCTCTTAATCTGTTGGGC+TGG | + | chr7.3:44822098-44822117 | None:intergenic | 50.0% | |
| ACTGAATGGGGGTCTCATGG+TGG | - | chr7.3:44822620-44822639 | MS.gene64572:CDS | 55.0% | |
| ACTGAGGAGCCAGCAGCATA+TGG | - | chr7.3:44822341-44822360 | MS.gene64572:intron | 55.0% | |
| TGGACTGAATGGGGGTCTCA+TGG | - | chr7.3:44822617-44822636 | MS.gene64572:CDS | 55.0% | |
| !! | TGACTTGGGCACCGACAACA+TGG | + | chr7.3:44822444-44822463 | None:intergenic | 55.0% |
| !! | CTGTTGGGCTGGCCCTGCCA+GGG | + | chr7.3:44822087-44822106 | None:intergenic | 70.0% |
| !! | TCTGTTGGGCTGGCCCTGCC+AGG | + | chr7.3:44822088-44822107 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 44821485 | 44822656 | 44821485 | ID=MS.gene64572 |
| chr7.3 | mRNA | 44821485 | 44822656 | 44821485 | ID=MS.gene64572.t1;Parent=MS.gene64572 |
| chr7.3 | exon | 44822546 | 44822656 | 44822546 | ID=MS.gene64572.t1.exon1;Parent=MS.gene64572.t1 |
| chr7.3 | CDS | 44822546 | 44822656 | 44822546 | ID=cds.MS.gene64572.t1;Parent=MS.gene64572.t1 |
| chr7.3 | exon | 44821648 | 44821848 | 44821648 | ID=MS.gene64572.t1.exon2;Parent=MS.gene64572.t1 |
| chr7.3 | CDS | 44821648 | 44821848 | 44821648 | ID=cds.MS.gene64572.t1;Parent=MS.gene64572.t1 |
| chr7.3 | exon | 44821485 | 44821535 | 44821485 | ID=MS.gene64572.t1.exon3;Parent=MS.gene64572.t1 |
| chr7.3 | CDS | 44821485 | 44821535 | 44821485 | ID=cds.MS.gene64572.t1;Parent=MS.gene64572.t1 |
| Gene Sequence |
| Protein sequence |
