Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65221.t1 | KEH25024.1 | 79.1 | 177 | 23 | 1 | 72 | 248 | 50 | 212 | 1.30E-74 | 289.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65221.t1 | A0A072U5J3 | 79.1 | 177 | 23 | 1 | 72 | 248 | 50 | 212 | 9.6e-75 | 289.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene65221 | 0.811564 | 6.57E-51 | -1.69E-46 |
| MS.gene049435 | MS.gene65221 | 0.804923 | 1.71E-49 | -1.69E-46 |
| MS.gene049501 | MS.gene65221 | 0.806896 | 6.58E-50 | -1.69E-46 |
| MS.gene050226 | MS.gene65221 | 0.801318 | 9.53E-49 | -1.69E-46 |
| MS.gene050227 | MS.gene65221 | 0.803002 | 4.29E-49 | -1.69E-46 |
| MS.gene050228 | MS.gene65221 | 0.816478 | 5.42E-52 | -1.69E-46 |
| MS.gene050237 | MS.gene65221 | 0.81164 | 6.33E-51 | -1.69E-46 |
| MS.gene050442 | MS.gene65221 | 0.831279 | 1.83E-55 | -1.69E-46 |
| MS.gene050719 | MS.gene65221 | 0.800237 | 1.58E-48 | -1.69E-46 |
| MS.gene050721 | MS.gene65221 | 0.801626 | 8.24E-49 | -1.69E-46 |
| MS.gene050750 | MS.gene65221 | 0.820625 | 6.21E-53 | -1.69E-46 |
| MS.gene050815 | MS.gene65221 | 0.800322 | 1.52E-48 | -1.69E-46 |
| MS.gene050953 | MS.gene65221 | 0.834171 | 3.50E-56 | -1.69E-46 |
| MS.gene051198 | MS.gene65221 | 0.804602 | 2.00E-49 | -1.69E-46 |
| MS.gene051243 | MS.gene65221 | 0.805429 | 1.34E-49 | -1.69E-46 |
| MS.gene052426 | MS.gene65221 | 0.807211 | 5.65E-50 | -1.69E-46 |
| MS.gene05288 | MS.gene65221 | 0.80254 | 5.34E-49 | -1.69E-46 |
| MS.gene053006 | MS.gene65221 | 0.805246 | 1.46E-49 | -1.69E-46 |
| MS.gene053008 | MS.gene65221 | 0.808949 | 2.41E-50 | -1.69E-46 |
| MS.gene05415 | MS.gene65221 | 0.820376 | 7.09E-53 | -1.69E-46 |
| MS.gene054405 | MS.gene65221 | 0.820181 | 7.86E-53 | -1.69E-46 |
| MS.gene054472 | MS.gene65221 | 0.811803 | 5.83E-51 | -1.69E-46 |
| MS.gene054657 | MS.gene65221 | 0.806161 | 9.41E-50 | -1.69E-46 |
| MS.gene054658 | MS.gene65221 | 0.8019 | 7.24E-49 | -1.69E-46 |
| MS.gene054930 | MS.gene65221 | 0.803969 | 2.71E-49 | -1.69E-46 |
| MS.gene05510 | MS.gene65221 | 0.814332 | 1.63E-51 | -1.69E-46 |
| MS.gene055608 | MS.gene65221 | 0.818346 | 2.06E-52 | -1.69E-46 |
| MS.gene055623 | MS.gene65221 | 0.805075 | 1.59E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene65221 | 0.804604 | 1.99E-49 | -1.69E-46 |
| MS.gene056506 | MS.gene65221 | 0.80263 | 5.12E-49 | -1.69E-46 |
| MS.gene056760 | MS.gene65221 | 0.801847 | 7.42E-49 | -1.69E-46 |
| MS.gene057869 | MS.gene65221 | 0.802111 | 6.55E-49 | -1.69E-46 |
| MS.gene058146 | MS.gene65221 | 0.82626 | 2.99E-54 | -1.69E-46 |
| MS.gene058548 | MS.gene65221 | 0.800246 | 1.58E-48 | -1.69E-46 |
| MS.gene058595 | MS.gene65221 | 0.805039 | 1.62E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene65221 | 0.805398 | 1.36E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene65221 | 0.801094 | 1.06E-48 | -1.69E-46 |
| MS.gene059842 | MS.gene65221 | 0.820558 | 6.44E-53 | -1.69E-46 |
| MS.gene060310 | MS.gene65221 | 0.828545 | 8.47E-55 | -1.69E-46 |
| MS.gene060322 | MS.gene65221 | 0.814187 | 1.75E-51 | -1.69E-46 |
| MS.gene060572 | MS.gene65221 | 0.80865 | 2.79E-50 | -1.69E-46 |
| MS.gene060683 | MS.gene65221 | 0.824423 | 8.14E-54 | -1.69E-46 |
| MS.gene060685 | MS.gene65221 | 0.819574 | 1.08E-52 | -1.69E-46 |
| MS.gene060803 | MS.gene65221 | 0.80154 | 8.58E-49 | -1.69E-46 |
| MS.gene060918 | MS.gene65221 | 0.804933 | 1.70E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65221.t1 | MTR_6g013220 | 79.070 | 172 | 22 | 1 | 77 | 248 | 55 | 212 | 2.13e-96 | 281 |
| MS.gene65221.t1 | MTR_6g048070 | 45.545 | 101 | 37 | 2 | 1 | 85 | 1 | 99 | 5.49e-19 | 80.1 |
| MS.gene65221.t1 | MTR_6g016175 | 26.693 | 251 | 157 | 8 | 1 | 243 | 1 | 232 | 1.44e-16 | 79.0 |
| MS.gene65221.t1 | MTR_3g448380 | 30.625 | 160 | 107 | 3 | 59 | 214 | 117 | 276 | 2.76e-14 | 71.2 |
| MS.gene65221.t1 | MTR_1g040450 | 30.409 | 171 | 113 | 4 | 76 | 243 | 111 | 278 | 6.46e-14 | 70.9 |
| MS.gene65221.t1 | MTR_2g105880 | 24.370 | 238 | 165 | 5 | 10 | 243 | 67 | 293 | 1.81e-13 | 69.3 |
| MS.gene65221.t1 | MTR_6g048840 | 28.000 | 175 | 112 | 5 | 76 | 243 | 225 | 392 | 2.05e-13 | 69.7 |
| MS.gene65221.t1 | MTR_7g108760 | 25.926 | 189 | 133 | 4 | 59 | 243 | 357 | 542 | 2.13e-13 | 69.7 |
| MS.gene65221.t1 | MTR_8g058470 | 25.926 | 189 | 133 | 4 | 59 | 243 | 355 | 540 | 2.29e-13 | 69.7 |
| MS.gene65221.t1 | MTR_3g022950 | 25.773 | 194 | 134 | 4 | 3 | 193 | 636 | 822 | 1.69e-12 | 67.0 |
| MS.gene65221.t1 | MTR_1g054775 | 29.885 | 174 | 104 | 5 | 76 | 238 | 618 | 784 | 7.70e-12 | 65.5 |
| MS.gene65221.t1 | MTR_7g083690 | 25.911 | 247 | 146 | 6 | 1 | 243 | 604 | 817 | 1.62e-11 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 45 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCAAGAGAGATTTGATTTA+TGG | 0.116291 | 6.4:-6717003 | MS.gene65221:CDS |
| TCGTCATTGGAACATGTTTC+TGG | 0.158342 | 6.4:+6717181 | None:intergenic |
| AAATCGCCAAACATCATTAA+AGG | 0.271490 | 6.4:+6716944 | None:intergenic |
| TCTTGACGTGTCTTTCCTAT+TGG | 0.326012 | 6.4:-6717044 | MS.gene65221:CDS |
| TCAACACCTTTAATGATGTT+TGG | 0.344659 | 6.4:-6716950 | MS.gene65221:CDS |
| TCATTACCCTTCAGTTCTTC+CGG | 0.370464 | 6.4:+6716905 | None:intergenic |
| AACAAATAAGAGATACATCT+TGG | 0.380955 | 6.4:-6717092 | MS.gene65221:CDS |
| AGGTGTAATCCAATTGTGTT+AGG | 0.387593 | 6.4:-6717292 | MS.gene65221:CDS |
| CGCCCTCTATTAGTTCAATC+TGG | 0.398255 | 6.4:-6716674 | MS.gene65221:CDS |
| TTGACCAAGCCTAACACAAT+TGG | 0.398918 | 6.4:+6717283 | None:intergenic |
| CCTTGTGGAGCTTCCGTTGT+TGG | 0.424252 | 6.4:-6716831 | MS.gene65221:CDS |
| GACAGGGATTGGTATTCTCT+TGG | 0.442717 | 6.4:-6717147 | MS.gene65221:CDS |
| GAACCAGATTGAACTAATAG+AGG | 0.449982 | 6.4:+6716671 | None:intergenic |
| GTAGGAGGGATTACTGAGTT+AGG | 0.459744 | 6.4:-6717346 | MS.gene65221:CDS |
| AACTCGGTGCATGAACCAAT+AGG | 0.486014 | 6.4:+6717029 | None:intergenic |
| AGGAGGAGAACAATGTAAGC+TGG | 0.493023 | 6.4:-6717213 | MS.gene65221:CDS |
| AAGAGATACATCTTGGTTGA+AGG | 0.497593 | 6.4:-6717085 | MS.gene65221:CDS |
| GAAGGAAAATTGATTCAAGA+AGG | 0.514667 | 6.4:-6717067 | MS.gene65221:CDS |
| GGAATGGCTAGAGCATTTCA+AGG | 0.514887 | 6.4:-6716738 | MS.gene65221:CDS |
| TACACCTATGAGCAACCTTA+CGG | 0.518280 | 6.4:+6717308 | None:intergenic |
| TGATCCGTAAGGTTGCTCAT+AGG | 0.529474 | 6.4:-6717312 | MS.gene65221:CDS |
| TAATCCAATTGTGTTAGGCT+TGG | 0.530247 | 6.4:-6717287 | MS.gene65221:CDS |
| CTCTATCTATTCTGCCTGCA+CGG | 0.531142 | 6.4:+6716774 | None:intergenic |
| GACTATGACAATAACCGTGC+AGG | 0.543680 | 6.4:-6716788 | MS.gene65221:CDS |
| TGTTCCAATGACGAGAAAAG+TGG | 0.545436 | 6.4:-6717172 | MS.gene65221:CDS |
| GTTCCAATGACGAGAAAAGT+GGG | 0.548017 | 6.4:-6717171 | MS.gene65221:CDS |
| GATGACATTACAAGCGTATG+TGG | 0.548253 | 6.4:-6716644 | MS.gene65221:CDS |
| TTTGGAACCGGAAGAACTGA+AGG | 0.550810 | 6.4:-6716912 | MS.gene65221:CDS |
| GGAGGAGAACAATGTAAGCT+GGG | 0.576028 | 6.4:-6717212 | MS.gene65221:CDS |
| AGTTAAAAGATGGTGGAAGG+AGG | 0.580076 | 6.4:-6717230 | MS.gene65221:CDS |
| GGTAATGAGGTGACTTCACA+AGG | 0.581563 | 6.4:-6716890 | MS.gene65221:CDS |
| TCATTTCAGCTATACAATGT+CGG | 0.588108 | 6.4:+6716855 | None:intergenic |
| AAAAGTTAAAAGATGGTGGA+AGG | 0.594675 | 6.4:-6717233 | MS.gene65221:CDS |
| AATCTCTCTTGACTGCAACT+CGG | 0.607370 | 6.4:+6717013 | None:intergenic |
| GGAAGAACTGAAGGGTAATG+AGG | 0.607937 | 6.4:-6716903 | MS.gene65221:CDS |
| AAAATCTGCGATGATCCGTA+AGG | 0.634575 | 6.4:-6717323 | MS.gene65221:CDS |
| AACCAGATTGAACTAATAGA+GGG | 0.638427 | 6.4:+6716672 | None:intergenic |
| TTGGAACCGGAAGAACTGAA+GGG | 0.662612 | 6.4:-6716911 | MS.gene65221:CDS |
| GTGAACTTTCTGTCCAACAA+CGG | 0.668255 | 6.4:+6716818 | None:intergenic |
| TCCAATGACGAGAAAAGTGG+GGG | 0.678217 | 6.4:-6717169 | MS.gene65221:CDS |
| TTCCAATGACGAGAAAAGTG+GGG | 0.679735 | 6.4:-6717170 | MS.gene65221:CDS |
| TGTTGGACAGAAAGTTCACA+TGG | 0.688863 | 6.4:-6716814 | MS.gene65221:CDS |
| CCAACAACGGAAGCTCCACA+AGG | 0.688911 | 6.4:+6716831 | None:intergenic |
| TATAGCTGAAATGAACCTTG+TGG | 0.699115 | 6.4:-6716846 | MS.gene65221:CDS |
| CAGATTGAACTAATAGAGGG+CGG | 0.706620 | 6.4:+6716675 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACAAATAAGAGATACATCT+TGG | - | chr6.4:6716912-6716931 | MS.gene65221:CDS | 25.0% |
| ! | ATGAAAAAGTTAAAAGATGG+TGG | - | chr6.4:6716767-6716786 | MS.gene65221:CDS | 25.0% |
| !!! | CCATCTTTTAACTTTTTCAT+CGG | + | chr6.4:6716767-6716786 | None:intergenic | 25.0% |
| AAATCGCCAAACATCATTAA+AGG | + | chr6.4:6717063-6717082 | None:intergenic | 30.0% | |
| AACCAGATTGAACTAATAGA+GGG | + | chr6.4:6717335-6717354 | None:intergenic | 30.0% | |
| CCGATGAAAAAGTTAAAAGA+TGG | - | chr6.4:6716764-6716783 | MS.gene65221:CDS | 30.0% | |
| TCAACACCTTTAATGATGTT+TGG | - | chr6.4:6717054-6717073 | MS.gene65221:CDS | 30.0% | |
| TCATTTCAGCTATACAATGT+CGG | + | chr6.4:6717152-6717171 | None:intergenic | 30.0% | |
| ! | AGATAGAGTTTTTGTAGATC+AGG | - | chr6.4:6717245-6717264 | MS.gene65221:CDS | 30.0% |
| ! | GAAGGAAAATTGATTCAAGA+AGG | - | chr6.4:6716937-6716956 | MS.gene65221:CDS | 30.0% |
| ! | GTCAAGAGAGATTTGATTTA+TGG | - | chr6.4:6717001-6717020 | MS.gene65221:CDS | 30.0% |
| !! | AAAAGTTAAAAGATGGTGGA+AGG | - | chr6.4:6716771-6716790 | MS.gene65221:CDS | 30.0% |
| !!! | TTTTCAAGAAGTTTTGGAAC+CGG | - | chr6.4:6717080-6717099 | MS.gene65221:CDS | 30.0% |
| AAGAGATACATCTTGGTTGA+AGG | - | chr6.4:6716919-6716938 | MS.gene65221:CDS | 35.0% | |
| AGGTGTAATCCAATTGTGTT+AGG | - | chr6.4:6716712-6716731 | MS.gene65221:CDS | 35.0% | |
| GAACCAGATTGAACTAATAG+AGG | + | chr6.4:6717336-6717355 | None:intergenic | 35.0% | |
| TAATCCAATTGTGTTAGGCT+TGG | - | chr6.4:6716717-6716736 | MS.gene65221:CDS | 35.0% | |
| TATAGCTGAAATGAACCTTG+TGG | - | chr6.4:6717158-6717177 | MS.gene65221:CDS | 35.0% | |
| ! | GAGTTTTTGTAGATCAGGAA+TGG | - | chr6.4:6717250-6717269 | MS.gene65221:CDS | 35.0% |
| !! | TGGCGATTTTCAAGAAGTTT+TGG | - | chr6.4:6717074-6717093 | MS.gene65221:CDS | 35.0% |
| !!! | AGGATTTTAGGTTGGAATGT+AGG | - | chr6.4:6716640-6716659 | MS.gene65221:CDS | 35.0% |
| !!! | ATTTCAAGGTTTTGAAGCTG+CGG | - | chr6.4:6717280-6717299 | MS.gene65221:CDS | 35.0% |
| !!! | ATTTTAGGTTGGAATGTAGG+AGG | - | chr6.4:6716643-6716662 | MS.gene65221:CDS | 35.0% |
| !!! | TTTTAGGTTGGAATGTAGGA+GGG | - | chr6.4:6716644-6716663 | MS.gene65221:CDS | 35.0% |
| AAAATCTGCGATGATCCGTA+AGG | - | chr6.4:6716681-6716700 | MS.gene65221:CDS | 40.0% | |
| AATCTCTCTTGACTGCAACT+CGG | + | chr6.4:6716994-6717013 | None:intergenic | 40.0% | |
| CAGATTGAACTAATAGAGGG+CGG | + | chr6.4:6717332-6717351 | None:intergenic | 40.0% | |
| GATGACATTACAAGCGTATG+TGG | - | chr6.4:6717360-6717379 | MS.gene65221:CDS | 40.0% | |
| GTGAACTTTCTGTCCAACAA+CGG | + | chr6.4:6717189-6717208 | None:intergenic | 40.0% | |
| GTTCCAATGACGAGAAAAGT+GGG | - | chr6.4:6716833-6716852 | MS.gene65221:CDS | 40.0% | |
| TACACCTATGAGCAACCTTA+CGG | + | chr6.4:6716699-6716718 | None:intergenic | 40.0% | |
| TCATTACCCTTCAGTTCTTC+CGG | + | chr6.4:6717102-6717121 | None:intergenic | 40.0% | |
| TCGTCATTGGAACATGTTTC+TGG | + | chr6.4:6716826-6716845 | None:intergenic | 40.0% | |
| TCTTGACGTGTCTTTCCTAT+TGG | - | chr6.4:6716960-6716979 | MS.gene65221:CDS | 40.0% | |
| TGTTCCAATGACGAGAAAAG+TGG | - | chr6.4:6716832-6716851 | MS.gene65221:CDS | 40.0% | |
| TGTTGGACAGAAAGTTCACA+TGG | - | chr6.4:6717190-6717209 | MS.gene65221:CDS | 40.0% | |
| TTCCAATGACGAGAAAAGTG+GGG | - | chr6.4:6716834-6716853 | MS.gene65221:CDS | 40.0% | |
| TTGACCAAGCCTAACACAAT+TGG | + | chr6.4:6716724-6716743 | None:intergenic | 40.0% | |
| !! | AGTTAAAAGATGGTGGAAGG+AGG | - | chr6.4:6716774-6716793 | MS.gene65221:CDS | 40.0% |
| !!! | TTTCAAGGTTTTGAAGCTGC+GGG | - | chr6.4:6717281-6717300 | MS.gene65221:CDS | 40.0% |
| AGGAGGAGAACAATGTAAGC+TGG | - | chr6.4:6716791-6716810 | MS.gene65221:CDS | 45.0% | |
| CGCCCTCTATTAGTTCAATC+TGG | - | chr6.4:6717330-6717349 | MS.gene65221:CDS | 45.0% | |
| CTCTATCTATTCTGCCTGCA+CGG | + | chr6.4:6717233-6717252 | None:intergenic | 45.0% | |
| GACTATGACAATAACCGTGC+AGG | - | chr6.4:6717216-6717235 | MS.gene65221:CDS | 45.0% | |
| GGAAGAACTGAAGGGTAATG+AGG | - | chr6.4:6717101-6717120 | MS.gene65221:CDS | 45.0% | |
| GGAGGAGAACAATGTAAGCT+GGG | - | chr6.4:6716792-6716811 | MS.gene65221:CDS | 45.0% | |
| GGTAATGAGGTGACTTCACA+AGG | - | chr6.4:6717114-6717133 | MS.gene65221:CDS | 45.0% | |
| GTAGGAGGGATTACTGAGTT+AGG | - | chr6.4:6716658-6716677 | MS.gene65221:CDS | 45.0% | |
| TCCAATGACGAGAAAAGTGG+GGG | - | chr6.4:6716835-6716854 | MS.gene65221:CDS | 45.0% | |
| TGATCCGTAAGGTTGCTCAT+AGG | - | chr6.4:6716692-6716711 | MS.gene65221:CDS | 45.0% | |
| TTGGAACCGGAAGAACTGAA+GGG | - | chr6.4:6717093-6717112 | MS.gene65221:CDS | 45.0% | |
| ! | GACAGGGATTGGTATTCTCT+TGG | - | chr6.4:6716857-6716876 | MS.gene65221:CDS | 45.0% |
| ! | GGAATGGCTAGAGCATTTCA+AGG | - | chr6.4:6717266-6717285 | MS.gene65221:CDS | 45.0% |
| ! | TTTGGAACCGGAAGAACTGA+AGG | - | chr6.4:6717092-6717111 | MS.gene65221:CDS | 45.0% |
| !! | AACTCGGTGCATGAACCAAT+AGG | + | chr6.4:6716978-6716997 | None:intergenic | 45.0% |
| !!! | TTCAAGGTTTTGAAGCTGCG+GGG | - | chr6.4:6717282-6717301 | MS.gene65221:CDS | 45.0% |
| !! | TCCCCCACTTTTCTCGTCAT+TGG | + | chr6.4:6716839-6716858 | None:intergenic | 50.0% |
| CCAACAACGGAAGCTCCACA+AGG | + | chr6.4:6717176-6717195 | None:intergenic | 55.0% | |
| CCTTGTGGAGCTTCCGTTGT+TGG | - | chr6.4:6717173-6717192 | MS.gene65221:CDS | 55.0% | |
| GAAAAGTGGGGGACAGGGAT+TGG | - | chr6.4:6716846-6716865 | MS.gene65221:CDS | 55.0% | |
| GACGAGAAAAGTGGGGGACA+GGG | - | chr6.4:6716841-6716860 | MS.gene65221:CDS | 55.0% | |
| TGACGAGAAAAGTGGGGGAC+AGG | - | chr6.4:6716840-6716859 | MS.gene65221:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 6716637 | 6717389 | 6716637 | ID=MS.gene65221 |
| chr6.4 | mRNA | 6716637 | 6717389 | 6716637 | ID=MS.gene65221.t1;Parent=MS.gene65221 |
| chr6.4 | exon | 6716637 | 6717389 | 6716637 | ID=MS.gene65221.t1.exon1;Parent=MS.gene65221.t1 |
| chr6.4 | CDS | 6716637 | 6717389 | 6716637 | ID=cds.MS.gene65221.t1;Parent=MS.gene65221.t1 |
| Gene Sequence |
| Protein sequence |
