Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65948.t1 | XP_003590039.1 | 98.9 | 537 | 6 | 0 | 1 | 537 | 1 | 537 | 2.50E-304 | 1053.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65948.t1 | O48639 | 81.2 | 516 | 95 | 2 | 4 | 519 | 5 | 518 | 9.8e-241 | 833.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65948.t1 | G7I5D0 | 98.9 | 537 | 6 | 0 | 1 | 537 | 1 | 537 | 1.8e-304 | 1053.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene65948 | 0.846785 | 1.75E-59 | -1.69E-46 |
| MS.gene050095 | MS.gene65948 | 0.807647 | 4.56E-50 | -1.69E-46 |
| MS.gene050096 | MS.gene65948 | 0.8311 | 2.02E-55 | -1.69E-46 |
| MS.gene050647 | MS.gene65948 | 0.818729 | 1.68E-52 | -1.69E-46 |
| MS.gene050720 | MS.gene65948 | 0.839056 | 1.99E-57 | -1.69E-46 |
| MS.gene050722 | MS.gene65948 | 0.811309 | 7.47E-51 | -1.69E-46 |
| MS.gene050725 | MS.gene65948 | 0.806801 | 6.89E-50 | -1.69E-46 |
| MS.gene051127 | MS.gene65948 | 0.802652 | 5.07E-49 | -1.69E-46 |
| MS.gene051131 | MS.gene65948 | 0.835018 | 2.14E-56 | -1.69E-46 |
| MS.gene051138 | MS.gene65948 | 0.820223 | 7.68E-53 | -1.69E-46 |
| MS.gene051858 | MS.gene65948 | -0.810069 | 1.38E-50 | -1.69E-46 |
| MS.gene052211 | MS.gene65948 | 0.855986 | 4.37E-62 | -1.69E-46 |
| MS.gene052212 | MS.gene65948 | 0.810357 | 1.20E-50 | -1.69E-46 |
| MS.gene05240 | MS.gene65948 | 0.806705 | 7.23E-50 | -1.69E-46 |
| MS.gene05244 | MS.gene65948 | 0.816706 | 4.82E-52 | -1.69E-46 |
| MS.gene052606 | MS.gene65948 | 0.830174 | 3.41E-55 | -1.69E-46 |
| MS.gene053031 | MS.gene65948 | 0.822631 | 2.14E-53 | -1.69E-46 |
| MS.gene053403 | MS.gene65948 | 0.899725 | 1.49E-77 | -1.69E-46 |
| MS.gene053406 | MS.gene65948 | 0.882408 | 1.07E-70 | -1.69E-46 |
| MS.gene053420 | MS.gene65948 | 0.823279 | 1.51E-53 | -1.69E-46 |
| MS.gene053887 | MS.gene65948 | 0.808285 | 3.34E-50 | -1.69E-46 |
| MS.gene054146 | MS.gene65948 | 0.850582 | 1.55E-60 | -1.69E-46 |
| MS.gene054181 | MS.gene65948 | 0.828355 | 9.41E-55 | -1.69E-46 |
| MS.gene054421 | MS.gene65948 | 0.806032 | 1.00E-49 | -1.69E-46 |
| MS.gene054742 | MS.gene65948 | 0.817241 | 3.65E-52 | -1.69E-46 |
| MS.gene05483 | MS.gene65948 | 0.814425 | 1.55E-51 | -1.69E-46 |
| MS.gene055154 | MS.gene65948 | 0.80683 | 6.80E-50 | -1.69E-46 |
| MS.gene055240 | MS.gene65948 | 0.848528 | 5.79E-60 | -1.69E-46 |
| MS.gene056218 | MS.gene65948 | 0.846629 | 1.93E-59 | -1.69E-46 |
| MS.gene056590 | MS.gene65948 | 0.823743 | 1.18E-53 | -1.69E-46 |
| MS.gene056591 | MS.gene65948 | 0.823361 | 1.44E-53 | -1.69E-46 |
| MS.gene056748 | MS.gene65948 | 0.818234 | 2.18E-52 | -1.69E-46 |
| MS.gene05837 | MS.gene65948 | -0.811127 | 8.18E-51 | -1.69E-46 |
| MS.gene058603 | MS.gene65948 | 0.836054 | 1.17E-56 | -1.69E-46 |
| MS.gene059402 | MS.gene65948 | 0.805079 | 1.59E-49 | -1.69E-46 |
| MS.gene059902 | MS.gene65948 | -0.807118 | 5.91E-50 | -1.69E-46 |
| MS.gene059953 | MS.gene65948 | 0.816318 | 5.88E-52 | -1.69E-46 |
| MS.gene059955 | MS.gene65948 | 0.838877 | 2.21E-57 | -1.69E-46 |
| MS.gene060593 | MS.gene65948 | 0.802275 | 6.06E-49 | -1.69E-46 |
| MS.gene06077 | MS.gene65948 | -0.807321 | 5.35E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65948.t1 | MTR_1g043220 | 98.883 | 537 | 6 | 0 | 1 | 537 | 1 | 537 | 0.0 | 1092 |
| MS.gene65948.t1 | MTR_1g043290 | 98.308 | 532 | 9 | 0 | 1 | 532 | 1 | 532 | 0.0 | 1075 |
| MS.gene65948.t1 | MTR_1g043200 | 97.925 | 530 | 11 | 0 | 1 | 530 | 1 | 530 | 0.0 | 1066 |
| MS.gene65948.t1 | MTR_1g074930 | 85.199 | 527 | 78 | 0 | 1 | 527 | 1 | 527 | 0.0 | 948 |
| MS.gene65948.t1 | MTR_3g082700 | 78.346 | 508 | 109 | 1 | 4 | 511 | 5 | 511 | 0.0 | 840 |
| MS.gene65948.t1 | MTR_1g069935 | 75.382 | 524 | 125 | 2 | 4 | 527 | 5 | 524 | 0.0 | 823 |
| MS.gene65948.t1 | MTR_7g096870 | 75.188 | 532 | 128 | 2 | 4 | 535 | 5 | 532 | 0.0 | 817 |
| MS.gene65948.t1 | MTR_7g096880 | 77.165 | 508 | 110 | 2 | 4 | 511 | 5 | 506 | 0.0 | 802 |
| MS.gene65948.t1 | MTR_1g074940 | 75.930 | 511 | 105 | 4 | 4 | 511 | 5 | 500 | 0.0 | 793 |
| MS.gene65948.t1 | MTR_1g069930 | 68.093 | 514 | 161 | 2 | 4 | 517 | 5 | 515 | 0.0 | 741 |
| MS.gene65948.t1 | MTR_1g028600 | 61.742 | 528 | 187 | 5 | 5 | 532 | 3 | 515 | 0.0 | 644 |
| MS.gene65948.t1 | MTR_5g068140 | 57.798 | 545 | 203 | 6 | 1 | 528 | 1 | 535 | 0.0 | 615 |
| MS.gene65948.t1 | MTR_4g083960 | 54.120 | 534 | 219 | 11 | 1 | 528 | 1 | 514 | 0.0 | 536 |
| MS.gene65948.t1 | MTR_3g084110 | 24.295 | 461 | 279 | 18 | 68 | 515 | 69 | 472 | 1.89e-16 | 82.4 |
| MS.gene65948.t1 | MTR_7g005910 | 22.603 | 438 | 276 | 16 | 86 | 514 | 92 | 475 | 5.95e-14 | 74.7 |
| MS.gene65948.t1 | MTR_1g116660 | 21.396 | 444 | 269 | 15 | 89 | 514 | 97 | 478 | 1.91e-12 | 70.1 |
| MS.gene65948.t1 | MTR_7g446340 | 23.758 | 463 | 293 | 16 | 75 | 529 | 104 | 514 | 1.34e-11 | 67.4 |
| MS.gene65948.t1 | MTR_6g007340 | 23.758 | 463 | 293 | 16 | 75 | 529 | 104 | 514 | 1.34e-11 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65948.t1 | AT5G43360 | 81.202 | 516 | 95 | 2 | 4 | 519 | 5 | 518 | 0.0 | 858 |
| MS.gene65948.t1 | AT3G54700 | 80.233 | 516 | 97 | 2 | 4 | 519 | 5 | 515 | 0.0 | 853 |
| MS.gene65948.t1 | AT3G54700 | 80.233 | 516 | 97 | 2 | 4 | 519 | 5 | 515 | 0.0 | 853 |
| MS.gene65948.t1 | AT5G43350 | 80.039 | 516 | 101 | 2 | 4 | 519 | 5 | 518 | 0.0 | 851 |
| MS.gene65948.t1 | AT2G38940 | 77.778 | 513 | 113 | 1 | 4 | 516 | 5 | 516 | 0.0 | 840 |
| MS.gene65948.t1 | AT2G32830 | 75.954 | 524 | 124 | 2 | 7 | 529 | 8 | 530 | 0.0 | 829 |
| MS.gene65948.t1 | AT5G43370 | 79.845 | 516 | 102 | 2 | 4 | 519 | 5 | 518 | 0.0 | 819 |
| MS.gene65948.t1 | AT5G43370 | 79.845 | 516 | 102 | 2 | 4 | 519 | 5 | 518 | 0.0 | 819 |
| MS.gene65948.t1 | AT5G43340 | 65.957 | 517 | 157 | 4 | 7 | 521 | 9 | 508 | 0.0 | 707 |
| MS.gene65948.t1 | AT1G76430 | 48.983 | 541 | 249 | 12 | 4 | 534 | 3 | 526 | 1.81e-172 | 498 |
| MS.gene65948.t1 | AT1G20860 | 49.905 | 529 | 247 | 9 | 5 | 529 | 3 | 517 | 1.21e-169 | 491 |
| MS.gene65948.t1 | AT1G20860 | 49.905 | 529 | 247 | 9 | 5 | 529 | 55 | 569 | 5.43e-169 | 491 |
| MS.gene65948.t1 | AT4G08878 | 64.368 | 261 | 90 | 2 | 30 | 290 | 13 | 270 | 1.20e-113 | 338 |
| MS.gene65948.t1 | AT4G08895 | 55.714 | 140 | 30 | 1 | 321 | 460 | 23 | 130 | 8.49e-47 | 160 |
| MS.gene65948.t1 | AT2G43330 | 23.126 | 467 | 276 | 17 | 68 | 514 | 72 | 475 | 1.36e-11 | 67.4 |
Find 106 sgRNAs with CRISPR-Local
Find 134 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACATTCTTCTTTGCTAATTT+TGG | 0.173620 | 1.1:+27038729 | MS.gene65948:CDS |
| CACTTTGTTTCTTTAGGTTT+TGG | 0.174225 | 1.1:+27037875 | MS.gene65948:CDS |
| GTTGCTTCAGGACTCTCTTT+TGG | 0.216739 | 1.1:+27037826 | MS.gene65948:CDS |
| GCAGTACTGTTCCGGGTTAC+TGG | 0.231450 | 1.1:+27038553 | MS.gene65948:CDS |
| TGCGTTGTGCAGTACTGTTC+CGG | 0.245438 | 1.1:+27038545 | MS.gene65948:CDS |
| TGGCTACACTTTGTTTCTTT+AGG | 0.248800 | 1.1:+27037869 | MS.gene65948:CDS |
| GCCGTGTAACGAGCGGTTTC+TGG | 0.257283 | 1.1:-27038201 | None:intergenic |
| AGTTATTCTTTGGTTGGCTT+GGG | 0.257756 | 1.1:+27037743 | MS.gene65948:CDS |
| TGGTTGGCTTGGGGACAAAA+TGG | 0.266103 | 1.1:+27037753 | MS.gene65948:CDS |
| TATATTTGATCACAAATATA+AGG | 0.273690 | 1.1:+27038053 | MS.gene65948:CDS |
| CGACATGGTTTGGCTTTGTT+TGG | 0.282199 | 1.1:+27038369 | MS.gene65948:CDS |
| GTTGTTTGTTCTGTTGCTTC+AGG | 0.283215 | 1.1:+27037814 | MS.gene65948:CDS |
| GGTGCAATTGTTGGTGCATT+TGG | 0.288648 | 1.1:+27038846 | MS.gene65948:CDS |
| GATCCTACCAAGACTGATAA+AGG | 0.289352 | 1.1:+27038894 | MS.gene65948:CDS |
| TGCCTCAATTTGATTATGTT+TGG | 0.291088 | 1.1:+27038115 | MS.gene65948:CDS |
| CTAGCAGGCCAGTTATTCTT+TGG | 0.291929 | 1.1:+27037733 | MS.gene65948:CDS |
| AGCAAGAAAGATAATAGAAT+TGG | 0.292218 | 1.1:+27038684 | MS.gene65948:CDS |
| TTCACTACAATTGTGATTGC+TGG | 0.295521 | 1.1:+27037553 | MS.gene65948:CDS |
| ACACTTGGTGGAAGTGTTCC+TGG | 0.300768 | 1.1:-27037673 | None:intergenic |
| TCAGTCATGTCTGGAGAATT+AGG | 0.311053 | 1.1:+27037487 | None:intergenic |
| TCCCTTGTCACAAAGTTGTT+AGG | 0.314005 | 1.1:+27037616 | MS.gene65948:CDS |
| ACAACAAATGTTGTAGCATT+TGG | 0.339412 | 1.1:-27038753 | None:intergenic |
| ACAAAATGACTAGTGATAAA+AGG | 0.342369 | 1.1:+27038310 | MS.gene65948:CDS |
| CAGTTATTCTTTGGTTGGCT+TGG | 0.344269 | 1.1:+27037742 | MS.gene65948:CDS |
| AGTGTAGCCATAACACTCTT+TGG | 0.353685 | 1.1:-27037856 | None:intergenic |
| GTTATGCACTTTACTTGTTC+CGG | 0.355959 | 1.1:+27038983 | MS.gene65948:CDS |
| GTTCCTGGTCTTGTTGGATT+TGG | 0.357998 | 1.1:-27037658 | None:intergenic |
| GCGTTGTGCAGTACTGTTCC+GGG | 0.367157 | 1.1:+27038546 | MS.gene65948:CDS |
| AAAGGGTATCCAACCGGTAT+TGG | 0.377035 | 1.1:+27038912 | MS.gene65948:CDS |
| TTCTTGCTCCAATGATCATA+TGG | 0.386763 | 1.1:-27038669 | None:intergenic |
| CAAGCCAACCAAAGAATAAC+TGG | 0.392014 | 1.1:-27037741 | None:intergenic |
| GGAAGTGTTCCTGGTCTTGT+TGG | 0.393755 | 1.1:-27037664 | None:intergenic |
| TCCTTCTTGCTCAGTGGCCT+CGG | 0.397975 | 1.1:-27039060 | None:intergenic |
| ATTCGCAGCACGACATGGTT+TGG | 0.398280 | 1.1:+27038359 | MS.gene65948:CDS |
| AAGGGTATCCAACCGGTATT+GGG | 0.400842 | 1.1:+27038913 | MS.gene65948:CDS |
| AGTGTTCAATCAGCTGTAAC+AGG | 0.406112 | 1.1:+27037691 | MS.gene65948:CDS |
| CCCTTGTCACAAAGTTGTTA+GGG | 0.410280 | 1.1:+27037617 | MS.gene65948:CDS |
| TGGAGACTTATTCTTATGTT+CGG | 0.414572 | 1.1:+27038135 | MS.gene65948:CDS |
| GCAAGAAAGATAATAGAATT+GGG | 0.436422 | 1.1:+27038685 | MS.gene65948:CDS |
| CAATCACAATTGTAGTGAAA+TGG | 0.437274 | 1.1:-27037549 | None:intergenic |
| CTTGGTGTAATCAACTTTGT+TGG | 0.438140 | 1.1:+27038957 | MS.gene65948:CDS |
| TTGCCATACCATATGATCAT+TGG | 0.440480 | 1.1:+27038661 | MS.gene65948:CDS |
| AGCTGCAGCGGATATGTCTA+AGG | 0.440556 | 1.1:+27038248 | MS.gene65948:CDS |
| CAGGCCAGTTATTCTTTGGT+TGG | 0.441993 | 1.1:+27037737 | MS.gene65948:CDS |
| GTAACAAATTCAGTCATGTC+TGG | 0.451043 | 1.1:+27037478 | None:intergenic |
| TGGATACCCTTTATCAGTCT+TGG | 0.453429 | 1.1:-27038901 | None:intergenic |
| GGTTGGCTTGGGGACAAAAT+GGG | 0.456779 | 1.1:+27037754 | MS.gene65948:CDS |
| TGGTTTAACTCTTATTCTCA+TGG | 0.457780 | 1.1:+27037792 | MS.gene65948:CDS |
| GTAACAGGTGTTGCACTTGT+TGG | 0.458063 | 1.1:+27037706 | MS.gene65948:CDS |
| TGTTTGGAACATGTAGTACT+TGG | 0.462947 | 1.1:+27038385 | MS.gene65948:CDS |
| TGCTACAACATTTGTTGTAC+CGG | 0.469717 | 1.1:+27038758 | MS.gene65948:CDS |
| TCAGCTGCTGCAGGAAAGGC+AGG | 0.471662 | 1.1:+27038825 | MS.gene65948:CDS |
| AAGAACTCTCTTATCATGCT+TGG | 0.472490 | 1.1:+27038939 | MS.gene65948:CDS |
| TTGGTGTAATCAACTTTGTT+GGG | 0.474242 | 1.1:+27038958 | MS.gene65948:CDS |
| CATGGAATCTCAGCTGCTGC+AGG | 0.483171 | 1.1:+27038816 | MS.gene65948:CDS |
| CAGTAGTACGTCAATGCAGC+TGG | 0.487577 | 1.1:-27038165 | None:intergenic |
| TTCTTAATCCCAATACCGGT+TGG | 0.488319 | 1.1:-27038921 | None:intergenic |
| TTTACTTGTTCCGGAATCAA+AGG | 0.491533 | 1.1:+27038992 | MS.gene65948:CDS |
| GGCACTAACAATGATGCTGC+TGG | 0.492284 | 1.1:-27038096 | None:intergenic |
| TACAATTGTGATTGCTGGAA+TGG | 0.494773 | 1.1:+27037558 | MS.gene65948:CDS |
| CGCAATCGGGTGGATTCCTC+CGG | 0.495250 | 1.1:+27038464 | MS.gene65948:CDS |
| ATCCTACCAAGACTGATAAA+GGG | 0.497221 | 1.1:+27038895 | MS.gene65948:CDS |
| ACAATTGTGATTGCTGGAAT+GGG | 0.498862 | 1.1:+27037559 | MS.gene65948:CDS |
| GCACTTGTTGGAACACTAGC+AGG | 0.499060 | 1.1:+27037718 | MS.gene65948:CDS |
| CAGCTGCATTGACGTACTAC+TGG | 0.499570 | 1.1:+27038166 | MS.gene65948:CDS |
| TTACTTGTTCCGGAATCAAA+GGG | 0.501725 | 1.1:+27038993 | MS.gene65948:CDS |
| AGTTGAACTAGAAGTTGAAG+AGG | 0.503525 | 1.1:+27038278 | MS.gene65948:CDS |
| ATCAAGTCCAAAGAGTGTTA+TGG | 0.504430 | 1.1:+27037849 | MS.gene65948:CDS |
| GTTATTCTTTGGTTGGCTTG+GGG | 0.514207 | 1.1:+27037744 | MS.gene65948:CDS |
| AGAGTTCTTAATCCCAATAC+CGG | 0.517475 | 1.1:-27038925 | None:intergenic |
| GGTAGCGTTTATCGATTACA+TGG | 0.520286 | 1.1:+27038581 | MS.gene65948:CDS |
| AATCTCAGCTGCTGCAGGAA+AGG | 0.521693 | 1.1:+27038821 | MS.gene65948:CDS |
| AGTGATAAAAGGAATAGTTA+TGG | 0.524232 | 1.1:+27038321 | MS.gene65948:CDS |
| ACTGATAAAGGGTATCCAAC+CGG | 0.524868 | 1.1:+27038906 | MS.gene65948:CDS |
| AGGTTAAGGTCTACTTGTCA+TGG | 0.528893 | 1.1:+27038798 | MS.gene65948:CDS |
| TGGAATTGTTGCATTGATTG+TGG | 0.531332 | 1.1:+27038026 | MS.gene65948:CDS |
| GTTACAGCTGATTGAACACT+TGG | 0.537306 | 1.1:-27037688 | None:intergenic |
| GACATGATTGTAGCTGAAAG+AGG | 0.545050 | 1.1:-27037925 | None:intergenic |
| CTCCAAACATAATCAAATTG+AGG | 0.545090 | 1.1:-27038117 | None:intergenic |
| TGTTCCGGGTTACTGGTTCA+CGG | 0.545707 | 1.1:+27038560 | MS.gene65948:CDS |
| ACTAGAAGTTGAAGAGGAGA+AGG | 0.546615 | 1.1:+27038284 | MS.gene65948:CDS |
| CCCTAACAACTTTGTGACAA+GGG | 0.549878 | 1.1:-27037617 | None:intergenic |
| GAACGAAGGAGAAGGTGCCG+AGG | 0.555336 | 1.1:+27039043 | MS.gene65948:CDS |
| GGAATCAAAGGGAAAATCGT+TGG | 0.559818 | 1.1:+27039004 | MS.gene65948:CDS |
| GCCAGAAACCGCTCGTTACA+CGG | 0.562042 | 1.1:+27038200 | MS.gene65948:CDS |
| AGTGGAGAGAACGAAGGAGA+AGG | 0.564524 | 1.1:+27039035 | MS.gene65948:CDS |
| GGAAAGGCAGGTGCAATTGT+TGG | 0.566386 | 1.1:+27038837 | MS.gene65948:CDS |
| GCTCCAATGATCATATGGTA+TGG | 0.568949 | 1.1:-27038664 | None:intergenic |
| AGAAATGAATGCAATTCATG+AGG | 0.592937 | 1.1:+27038491 | MS.gene65948:CDS |
| TCCCTAACAACTTTGTGACA+AGG | 0.594495 | 1.1:-27037618 | None:intergenic |
| CAAGTAGACCTTAACCTAGC+CGG | 0.597095 | 1.1:-27038792 | None:intergenic |
| ACAGCTGATTGAACACTTGG+TGG | 0.608784 | 1.1:-27037685 | None:intergenic |
| GCCGAGGCCACTGAGCAAGA+AGG | 0.609359 | 1.1:+27039059 | MS.gene65948:CDS |
| CCGCGGTCCTTCTTGCTCAG+TGG | 0.613065 | 1.1:-27039066 | None:intergenic |
| AAGCAATTCGCAGCACGACA+TGG | 0.614851 | 1.1:+27038354 | MS.gene65948:CDS |
| TACCCTTTATCAGTCTTGGT+AGG | 0.618148 | 1.1:-27038897 | None:intergenic |
| ACATGATTGTAGCTGAAAGA+GGG | 0.622142 | 1.1:-27037924 | None:intergenic |
| GAGTTGAGTGGAGAGAACGA+AGG | 0.632179 | 1.1:+27039029 | MS.gene65948:CDS |
| GAACGCGAAACAAGCTGCAG+CGG | 0.635840 | 1.1:+27038236 | MS.gene65948:CDS |
| GAACCAAATCCAACAAGACC+AGG | 0.636167 | 1.1:+27037655 | MS.gene65948:CDS |
| AAATCGTTGGAAGAGTTGAG+TGG | 0.638926 | 1.1:+27039017 | MS.gene65948:CDS |
| GCTACCGTGAACCAGTAACC+CGG | 0.646440 | 1.1:-27038564 | None:intergenic |
| AACAAGAGCCGTGTAACGAG+CGG | 0.663708 | 1.1:-27038208 | None:intergenic |
| AGTGCTAAATGCACTTGATG+TGG | 0.688145 | 1.1:+27037510 | MS.gene65948:CDS |
| GTAGCGTTTATCGATTACAT+GGG | 0.697486 | 1.1:+27038582 | MS.gene65948:CDS |
| CCACTGAGCAAGAAGGACCG+CGG | 0.738705 | 1.1:+27039066 | MS.gene65948:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATATTTGATCACAAATATA+AGG | + | chr1.1:27038053-27038072 | MS.gene65948:CDS | 15.0% |
| ! | ACAAAATGACTAGTGATAAA+AGG | + | chr1.1:27038310-27038329 | MS.gene65948:CDS | 25.0% |
| ! | AGCAAGAAAGATAATAGAAT+TGG | + | chr1.1:27038684-27038703 | MS.gene65948:CDS | 25.0% |
| ! | AGTGATAAAAGGAATAGTTA+TGG | + | chr1.1:27038321-27038340 | MS.gene65948:CDS | 25.0% |
| ! | GCAAGAAAGATAATAGAATT+GGG | + | chr1.1:27038685-27038704 | MS.gene65948:CDS | 25.0% |
| ! | TATGCTAACAAAAAAACTAG+AGG | + | chr1.1:27037952-27037971 | MS.gene65948:CDS | 25.0% |
| !! | ACATTCTTCTTTGCTAATTT+TGG | + | chr1.1:27038729-27038748 | MS.gene65948:CDS | 25.0% |
| !! | ATGGGAAGAAAAAAAGTATA+TGG | + | chr1.1:27037772-27037791 | MS.gene65948:CDS | 25.0% |
| ACAACAAATGTTGTAGCATT+TGG | - | chr1.1:27038756-27038775 | None:intergenic | 30.0% | |
| AGAAATGAATGCAATTCATG+AGG | + | chr1.1:27038491-27038510 | MS.gene65948:CDS | 30.0% | |
| CAATCACAATTGTAGTGAAA+TGG | - | chr1.1:27037552-27037571 | None:intergenic | 30.0% | |
| CTCCAAACATAATCAAATTG+AGG | - | chr1.1:27038120-27038139 | None:intergenic | 30.0% | |
| TGCCTCAATTTGATTATGTT+TGG | + | chr1.1:27038115-27038134 | MS.gene65948:CDS | 30.0% | |
| TGGAGACTTATTCTTATGTT+CGG | + | chr1.1:27038135-27038154 | MS.gene65948:CDS | 30.0% | |
| TGGTTTAACTCTTATTCTCA+TGG | + | chr1.1:27037792-27037811 | MS.gene65948:CDS | 30.0% | |
| TTGGTGTAATCAACTTTGTT+GGG | + | chr1.1:27038958-27038977 | MS.gene65948:CDS | 30.0% | |
| ! | ATTGCATTCATTTCTTTTGC+CGG | - | chr1.1:27038486-27038505 | None:intergenic | 30.0% |
| ! | TAGTCAGAATCTTTTCCAAA+AGG | + | chr1.1:27038431-27038450 | MS.gene65948:CDS | 30.0% |
| !! | ATGCAAGGTTTTGGTATATT+AGG | + | chr1.1:27038000-27038019 | MS.gene65948:CDS | 30.0% |
| !! | ATGTAGTACTTGGTTTTTGT+TGG | + | chr1.1:27038395-27038414 | MS.gene65948:CDS | 30.0% |
| !! | CACTTTGTTTCTTTAGGTTT+TGG | + | chr1.1:27037875-27037894 | MS.gene65948:CDS | 30.0% |
| !!! | CGTTTTGCTATTCAAATGAT+GGG | + | chr1.1:27038606-27038625 | MS.gene65948:CDS | 30.0% |
| !!! | TCGTTTTGCTATTCAAATGA+TGG | + | chr1.1:27038605-27038624 | MS.gene65948:CDS | 30.0% |
| AAGAACTCTCTTATCATGCT+TGG | + | chr1.1:27038939-27038958 | MS.gene65948:CDS | 35.0% | |
| ACAATTGTGATTGCTGGAAT+GGG | + | chr1.1:27037559-27037578 | MS.gene65948:CDS | 35.0% | |
| ACATGATTGTAGCTGAAAGA+GGG | - | chr1.1:27037927-27037946 | None:intergenic | 35.0% | |
| AGAGTTCTTAATCCCAATAC+CGG | - | chr1.1:27038928-27038947 | None:intergenic | 35.0% | |
| AGTTGAACTAGAAGTTGAAG+AGG | + | chr1.1:27038278-27038297 | MS.gene65948:CDS | 35.0% | |
| ATCCTACCAAGACTGATAAA+GGG | + | chr1.1:27038895-27038914 | MS.gene65948:CDS | 35.0% | |
| GTTATGCACTTTACTTGTTC+CGG | + | chr1.1:27038983-27039002 | MS.gene65948:CDS | 35.0% | |
| TACAATTGTGATTGCTGGAA+TGG | + | chr1.1:27037558-27037577 | MS.gene65948:CDS | 35.0% | |
| TTACTTGTTCCGGAATCAAA+GGG | + | chr1.1:27038993-27039012 | MS.gene65948:CDS | 35.0% | |
| TTCACTACAATTGTGATTGC+TGG | + | chr1.1:27037553-27037572 | MS.gene65948:CDS | 35.0% | |
| TTCTTGCTCCAATGATCATA+TGG | - | chr1.1:27038672-27038691 | None:intergenic | 35.0% | |
| TTGCCATACCATATGATCAT+TGG | + | chr1.1:27038661-27038680 | MS.gene65948:CDS | 35.0% | |
| TTTACTTGTTCCGGAATCAA+AGG | + | chr1.1:27038992-27039011 | MS.gene65948:CDS | 35.0% | |
| ! | AACGATTTTCCCTTTGATTC+CGG | - | chr1.1:27039005-27039024 | None:intergenic | 35.0% |
| ! | CTTGGTGTAATCAACTTTGT+TGG | + | chr1.1:27038957-27038976 | MS.gene65948:CDS | 35.0% |
| ! | GTAGCGTTTATCGATTACAT+GGG | + | chr1.1:27038582-27038601 | MS.gene65948:CDS | 35.0% |
| ! | TGGCTACACTTTGTTTCTTT+AGG | + | chr1.1:27037869-27037888 | MS.gene65948:CDS | 35.0% |
| ! | TGTTTGGAACATGTAGTACT+TGG | + | chr1.1:27038385-27038404 | MS.gene65948:CDS | 35.0% |
| ! | TTGCGCTAAAAATGTCCTTT+TGG | - | chr1.1:27038449-27038468 | None:intergenic | 35.0% |
| !! | AGTTATTCTTTGGTTGGCTT+GGG | + | chr1.1:27037743-27037762 | MS.gene65948:CDS | 35.0% |
| !! | ATCAAGTCCAAAGAGTGTTA+TGG | + | chr1.1:27037849-27037868 | MS.gene65948:CDS | 35.0% |
| !! | CAAGGTTTTGGTATATTAGG+TGG | + | chr1.1:27038003-27038022 | MS.gene65948:CDS | 35.0% |
| !! | GTTTTTGCTATGCAAGGTTT+TGG | + | chr1.1:27037991-27038010 | MS.gene65948:CDS | 35.0% |
| !! | TGCTACAACATTTGTTGTAC+CGG | + | chr1.1:27038758-27038777 | MS.gene65948:CDS | 35.0% |
| !! | TGGAATTGTTGCATTGATTG+TGG | + | chr1.1:27038026-27038045 | MS.gene65948:CDS | 35.0% |
| !!! | AAGGACATTTTTAGCGCAAT+CGG | + | chr1.1:27038450-27038469 | MS.gene65948:CDS | 35.0% |
| !!! | TGTTTCTTTAGGTTTTGGCT+TGG | + | chr1.1:27037880-27037899 | MS.gene65948:CDS | 35.0% |
| !!! | TTTAGGTTTTGGCTTGGTTT+TGG | + | chr1.1:27037886-27037905 | MS.gene65948:CDS | 35.0% |
| !!! | TTTTGGCTTGGTTTTGGTAT+TGG | + | chr1.1:27037892-27037911 | MS.gene65948:CDS | 35.0% |
| AAATCGTTGGAAGAGTTGAG+TGG | + | chr1.1:27039017-27039036 | MS.gene65948:CDS | 40.0% | |
| ACTAGAAGTTGAAGAGGAGA+AGG | + | chr1.1:27038284-27038303 | MS.gene65948:CDS | 40.0% | |
| AGGTTAAGGTCTACTTGTCA+TGG | + | chr1.1:27038798-27038817 | MS.gene65948:CDS | 40.0% | |
| AGTGCTAAATGCACTTGATG+TGG | + | chr1.1:27037510-27037529 | MS.gene65948:CDS | 40.0% | |
| AGTGTAGCCATAACACTCTT+TGG | - | chr1.1:27037859-27037878 | None:intergenic | 40.0% | |
| AGTGTTCAATCAGCTGTAAC+AGG | + | chr1.1:27037691-27037710 | MS.gene65948:CDS | 40.0% | |
| CAAGCCAACCAAAGAATAAC+TGG | - | chr1.1:27037744-27037763 | None:intergenic | 40.0% | |
| CCCTAACAACTTTGTGACAA+GGG | - | chr1.1:27037620-27037639 | None:intergenic | 40.0% | |
| CCCTTGTCACAAAGTTGTTA+GGG | + | chr1.1:27037617-27037636 | MS.gene65948:CDS | 40.0% | |
| GACATGATTGTAGCTGAAAG+AGG | - | chr1.1:27037928-27037947 | None:intergenic | 40.0% | |
| GATCCTACCAAGACTGATAA+AGG | + | chr1.1:27038894-27038913 | MS.gene65948:CDS | 40.0% | |
| GCTCCAATGATCATATGGTA+TGG | - | chr1.1:27038667-27038686 | None:intergenic | 40.0% | |
| GGAATCAAAGGGAAAATCGT+TGG | + | chr1.1:27039004-27039023 | MS.gene65948:CDS | 40.0% | |
| GTTACAGCTGATTGAACACT+TGG | - | chr1.1:27037691-27037710 | None:intergenic | 40.0% | |
| TACCCTTTATCAGTCTTGGT+AGG | - | chr1.1:27038900-27038919 | None:intergenic | 40.0% | |
| TCCCTAACAACTTTGTGACA+AGG | - | chr1.1:27037621-27037640 | None:intergenic | 40.0% | |
| TCCCTTGTCACAAAGTTGTT+AGG | + | chr1.1:27037616-27037635 | MS.gene65948:CDS | 40.0% | |
| TGGATACCCTTTATCAGTCT+TGG | - | chr1.1:27038904-27038923 | None:intergenic | 40.0% | |
| TTCTTAATCCCAATACCGGT+TGG | - | chr1.1:27038924-27038943 | None:intergenic | 40.0% | |
| ! | AGATTTTTCCGGCTAGGTTA+AGG | + | chr1.1:27038784-27038803 | MS.gene65948:CDS | 40.0% |
| ! | CAAGCTACATTTTCAAACCG+CGG | - | chr1.1:27039086-27039105 | None:intergenic | 40.0% |
| ! | CAGTTATTCTTTGGTTGGCT+TGG | + | chr1.1:27037742-27037761 | MS.gene65948:CDS | 40.0% |
| ! | GCTGGATTTTCTTCAAAGGT+GGG | - | chr1.1:27038081-27038100 | None:intergenic | 40.0% |
| ! | GGTAGCGTTTATCGATTACA+TGG | + | chr1.1:27038581-27038600 | MS.gene65948:CDS | 40.0% |
| ! | GTTGTTTGTTCTGTTGCTTC+AGG | + | chr1.1:27037814-27037833 | MS.gene65948:CDS | 40.0% |
| ! | TGCTGCTGGATTTTCTTCAA+AGG | - | chr1.1:27038085-27038104 | None:intergenic | 40.0% |
| ! | TGCTGGATTTTCTTCAAAGG+TGG | - | chr1.1:27038082-27038101 | None:intergenic | 40.0% |
| !! | ACTGATAAAGGGTATCCAAC+CGG | + | chr1.1:27038906-27038925 | MS.gene65948:CDS | 40.0% |
| !! | GGTTTTGGTATATTAGGTGG+TGG | + | chr1.1:27038006-27038025 | MS.gene65948:CDS | 40.0% |
| !! | GTTATTCTTTGGTTGGCTTG+GGG | + | chr1.1:27037744-27037763 | MS.gene65948:CDS | 40.0% |
| !!! | AGGACATTTTTAGCGCAATC+GGG | + | chr1.1:27038451-27038470 | MS.gene65948:CDS | 40.0% |
| ACAGCTGATTGAACACTTGG+TGG | - | chr1.1:27037688-27037707 | None:intergenic | 45.0% | |
| CAAGTAGACCTTAACCTAGC+CGG | - | chr1.1:27038795-27038814 | None:intergenic | 45.0% | |
| CAGGCCAGTTATTCTTTGGT+TGG | + | chr1.1:27037737-27037756 | MS.gene65948:CDS | 45.0% | |
| CTAGCAGGCCAGTTATTCTT+TGG | + | chr1.1:27037733-27037752 | MS.gene65948:CDS | 45.0% | |
| GAACCAAATCCAACAAGACC+AGG | + | chr1.1:27037655-27037674 | MS.gene65948:CDS | 45.0% | |
| GGTGCAATTGTTGGTGCATT+TGG | + | chr1.1:27038846-27038865 | MS.gene65948:CDS | 45.0% | |
| ! | GCATTCATTTCTTTTGCCGG+AGG | - | chr1.1:27038483-27038502 | None:intergenic | 45.0% |
| ! | GTAACAGGTGTTGCACTTGT+TGG | + | chr1.1:27037706-27037725 | MS.gene65948:CDS | 45.0% |
| ! | GTTGCTTCAGGACTCTCTTT+TGG | + | chr1.1:27037826-27037845 | MS.gene65948:CDS | 45.0% |
| !! | AAAGGGTATCCAACCGGTAT+TGG | + | chr1.1:27038912-27038931 | MS.gene65948:CDS | 45.0% |
| !! | AAGGGTATCCAACCGGTATT+GGG | + | chr1.1:27038913-27038932 | MS.gene65948:CDS | 45.0% |
| !! | CGACATGGTTTGGCTTTGTT+TGG | + | chr1.1:27038369-27038388 | MS.gene65948:CDS | 45.0% |
| !! | GTTCCTGGTCTTGTTGGATT+TGG | - | chr1.1:27037661-27037680 | None:intergenic | 45.0% |
| !! | TGTACCGGCAGAGATTTTTC+CGG | + | chr1.1:27038773-27038792 | MS.gene65948:CDS | 45.0% |
| !!! | ACATTTTTAGCGCAATCGGG+TGG | + | chr1.1:27038454-27038473 | MS.gene65948:CDS | 45.0% |
| !!! | GCTGCTGTTTTTGCTATGCA+AGG | + | chr1.1:27037985-27038004 | MS.gene65948:CDS | 45.0% |
| !!! | TGGCTTGGTTTTGGTATTGG+TGG | + | chr1.1:27037895-27037914 | MS.gene65948:CDS | 45.0% |
| AACAAGAGCCGTGTAACGAG+CGG | - | chr1.1:27038211-27038230 | None:intergenic | 50.0% | |
| AAGCAATTCGCAGCACGACA+TGG | + | chr1.1:27038354-27038373 | MS.gene65948:CDS | 50.0% | |
| AATCTCAGCTGCTGCAGGAA+AGG | + | chr1.1:27038821-27038840 | MS.gene65948:CDS | 50.0% | |
| AGCTGCAGCGGATATGTCTA+AGG | + | chr1.1:27038248-27038267 | MS.gene65948:CDS | 50.0% | |
| AGTGGAGAGAACGAAGGAGA+AGG | + | chr1.1:27039035-27039054 | MS.gene65948:CDS | 50.0% | |
| ATTCGCAGCACGACATGGTT+TGG | + | chr1.1:27038359-27038378 | MS.gene65948:CDS | 50.0% | |
| CAGTAGTACGTCAATGCAGC+TGG | - | chr1.1:27038168-27038187 | None:intergenic | 50.0% | |
| CTAGCCGGAAAAATCTCTGC+CGG | - | chr1.1:27038780-27038799 | None:intergenic | 50.0% | |
| GAGTTGAGTGGAGAGAACGA+AGG | + | chr1.1:27039029-27039048 | MS.gene65948:CDS | 50.0% | |
| GGAAAGGCAGGTGCAATTGT+TGG | + | chr1.1:27038837-27038856 | MS.gene65948:CDS | 50.0% | |
| GGCACTAACAATGATGCTGC+TGG | - | chr1.1:27038099-27038118 | None:intergenic | 50.0% | |
| TGCGTTGTGCAGTACTGTTC+CGG | + | chr1.1:27038545-27038564 | MS.gene65948:CDS | 50.0% | |
| ! | CAGCTGCATTGACGTACTAC+TGG | + | chr1.1:27038166-27038185 | MS.gene65948:CDS | 50.0% |
| ! | GCACTTGTTGGAACACTAGC+AGG | + | chr1.1:27037718-27037737 | MS.gene65948:CDS | 50.0% |
| !! | ACACTTGGTGGAAGTGTTCC+TGG | - | chr1.1:27037676-27037695 | None:intergenic | 50.0% |
| !! | GGAAGTGTTCCTGGTCTTGT+TGG | - | chr1.1:27037667-27037686 | None:intergenic | 50.0% |
| !! | GGTTGGCTTGGGGACAAAAT+GGG | + | chr1.1:27037754-27037773 | MS.gene65948:CDS | 50.0% |
| !! | TGGTTGGCTTGGGGACAAAA+TGG | + | chr1.1:27037753-27037772 | MS.gene65948:CDS | 50.0% |
| !! | TGTTCCGGGTTACTGGTTCA+CGG | + | chr1.1:27038560-27038579 | MS.gene65948:CDS | 50.0% |
| CATGGAATCTCAGCTGCTGC+AGG | + | chr1.1:27038816-27038835 | MS.gene65948:CDS | 55.0% | |
| GAACGCGAAACAAGCTGCAG+CGG | + | chr1.1:27038236-27038255 | MS.gene65948:CDS | 55.0% | |
| GCAGTACTGTTCCGGGTTAC+TGG | + | chr1.1:27038553-27038572 | MS.gene65948:CDS | 55.0% | |
| GCCAGAAACCGCTCGTTACA+CGG | + | chr1.1:27038200-27038219 | MS.gene65948:CDS | 55.0% | |
| GCGTTGTGCAGTACTGTTCC+GGG | + | chr1.1:27038546-27038565 | MS.gene65948:CDS | 55.0% | |
| GCTACCGTGAACCAGTAACC+CGG | - | chr1.1:27038567-27038586 | None:intergenic | 55.0% | |
| TCCTTCTTGCTCAGTGGCCT+CGG | - | chr1.1:27039063-27039082 | None:intergenic | 55.0% | |
| ! | CGGCAGAGATTTTTCCGGCT+AGG | + | chr1.1:27038778-27038797 | MS.gene65948:CDS | 55.0% |
| CCACTGAGCAAGAAGGACCG+CGG | + | chr1.1:27039066-27039085 | MS.gene65948:CDS | 60.0% | |
| GCCGTGTAACGAGCGGTTTC+TGG | - | chr1.1:27038204-27038223 | None:intergenic | 60.0% | |
| TCAGCTGCTGCAGGAAAGGC+AGG | + | chr1.1:27038825-27038844 | MS.gene65948:CDS | 60.0% | |
| ! | CGCAATCGGGTGGATTCCTC+CGG | + | chr1.1:27038464-27038483 | MS.gene65948:CDS | 60.0% |
| !! | GAACGAAGGAGAAGGTGCCG+AGG | + | chr1.1:27039043-27039062 | MS.gene65948:CDS | 60.0% |
| CCGCGGTCCTTCTTGCTCAG+TGG | - | chr1.1:27039069-27039088 | None:intergenic | 65.0% | |
| GCCGAGGCCACTGAGCAAGA+AGG | + | chr1.1:27039059-27039078 | MS.gene65948:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 27037493 | 27039106 | 27037493 | ID=MS.gene65948 |
| chr1.1 | mRNA | 27037493 | 27039106 | 27037493 | ID=MS.gene65948.t1;Parent=MS.gene65948 |
| chr1.1 | exon | 27037493 | 27039106 | 27037493 | ID=MS.gene65948.t1.exon1;Parent=MS.gene65948.t1 |
| chr1.1 | CDS | 27037493 | 27039106 | 27037493 | ID=cds.MS.gene65948.t1;Parent=MS.gene65948.t1 |
| Gene Sequence |
| Protein sequence |
