AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70596


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70596.t1 MTR_3g078370 96.296 270 9 1 1 269 82 351 0.0 515
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70596.t1 AT3G49080 57.563 238 86 5 42 269 144 376 5.89e-76 238

Find 72 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCTGGTAACGGTAAATTTA+TGG 0.138790 3.1:+64287029 MS.gene70596:CDS
GAAGAGGATGCTTTGTTTAA+AGG 0.164710 3.1:+64286259 MS.gene70596:CDS
GTGAAAGGTGGTGGTGTTTC+AGG 0.217283 3.1:+64287162 MS.gene70596:CDS
GGTGACTTGATTGCTGCTTC+TGG 0.228441 3.1:+64286760 MS.gene70596:CDS
GATGGTGAAAGTGGTGATTT+TGG 0.309890 3.1:+64286307 MS.gene70596:CDS
AAAATGGTGGTTAGAAGAAA+AGG 0.368505 3.1:+64286222 MS.gene70596:CDS
AGACTAAGACATTGGGGCTC+TGG 0.370218 3.1:+64287124 MS.gene70596:CDS
AGTGAGACAAGTAGATGATA+AGG 0.379415 3.1:+64286951 MS.gene70596:CDS
AAAGATCTTAAAGATGTTGA+TGG 0.382922 3.1:+64286820 MS.gene70596:CDS
TTCTCTGAGACTAAGACATT+GGG 0.395628 3.1:+64287117 MS.gene70596:CDS
AGAGGATGATGATGTGAGTA+AGG 0.404212 3.1:+64286411 MS.gene70596:CDS
CAAATCCACCAGAGGTGAAA+TGG 0.404466 3.1:+64286894 MS.gene70596:CDS
TGAAACACCACCACCTTTCA+CGG 0.413786 3.1:-64287160 None:intergenic
TGGAATTTGAAAGAGGATAC+TGG 0.418798 3.1:+64286370 MS.gene70596:CDS
TTTGTTTAAAGGAATTGATA+AGG 0.420765 3.1:+64286270 MS.gene70596:CDS
TAGTCTCAGAGAAAGGTCTA+AGG 0.428550 3.1:-64287107 None:intergenic
ATGCGCTATGAGAGGAATAC+TGG 0.445921 3.1:+64286871 MS.gene70596:CDS
GACTAAGACATTGGGGCTCT+GGG 0.446729 3.1:+64287125 MS.gene70596:CDS
TAGACGCTCCATTTCACCTC+TGG 0.447888 3.1:-64286902 None:intergenic
GTGAAGGAGGGGAGAGAGTG+AGG 0.453262 3.1:+64286179 MS.gene70596:CDS
AAGGGACGTGCCTATGGAAC+CGG 0.462628 3.1:+64286970 MS.gene70596:CDS
GGGATGAATGAAGGTGAAGG+AGG 0.464929 3.1:+64286166 None:intergenic
GTGAGACAAGTAGATGATAA+GGG 0.465382 3.1:+64286952 MS.gene70596:CDS
AGGAGGGGAGAGAGTGAGGA+AGG 0.465845 3.1:+64286183 MS.gene70596:CDS
TTTCTCTGAGACTAAGACAT+TGG 0.467627 3.1:+64287116 MS.gene70596:CDS
TATCTGCTCCAACACCAATA+TGG 0.469968 3.1:-64286333 None:intergenic
ACCATAAATTTACCGTTACC+AGG 0.475003 3.1:-64287030 None:intergenic
GGATGAATGAAGGTGAAGGA+GGG 0.475747 3.1:+64286167 None:intergenic
GATGATAAGGGACGTGCCTA+TGG 0.478544 3.1:+64286964 MS.gene70596:CDS
ACTGCAGGAACGAAAGCTTT+TGG 0.489898 3.1:+64286739 MS.gene70596:intron
TTTGGTGACCATATTGGTGT+TGG 0.496594 3.1:+64286325 MS.gene70596:CDS
ACGCTCCATTTCACCTCTGG+TGG 0.497185 3.1:-64286899 None:intergenic
CAGATAATGTTGATGAACCT+TGG 0.500891 3.1:+64286350 MS.gene70596:CDS
AAATGGTGGTTAGAAGAAAA+GGG 0.502327 3.1:+64286223 MS.gene70596:CDS
GCGTCTAAAGCAAGAGGAAG+CGG 0.519797 3.1:+64286918 MS.gene70596:CDS
TGAACCTTGGAATTTGAAAG+AGG 0.523153 3.1:+64286363 MS.gene70596:CDS
GTATCCTCTTTCAAATTCCA+AGG 0.528823 3.1:-64286367 None:intergenic
TTCTGAACTTCCTCTTGACT+CGG 0.532049 3.1:-64286466 None:intergenic
AAGGAAACTGAGGTTAAAGA+TGG 0.540127 3.1:+64286289 MS.gene70596:CDS
TTCTCTATTTCACTAAGAGG+TGG 0.550263 3.1:-64286847 None:intergenic
GAGCTCATTGCTGTTCTCGA+AGG 0.551281 3.1:+64286508 MS.gene70596:CDS
AGTAGCACGATGATCGAGCA+TGG 0.555558 3.1:-64287082 None:intergenic
AATGTCTTAGTCTCAGAGAA+AGG 0.565528 3.1:-64287114 None:intergenic
GGGTATTGATAATGAAGAAG+AGG 0.566295 3.1:+64286243 MS.gene70596:CDS
GCACGTGTGTGGGTTCAGCC+TGG 0.568099 3.1:+64287012 MS.gene70596:CDS
TGGTGAGCTTAATGTTGTCG+AGG 0.568877 3.1:+64286441 MS.gene70596:CDS
GGATGATGATGTGAGTAAGG+AGG 0.571155 3.1:+64286414 MS.gene70596:CDS
GTAGCACGATGATCGAGCAT+GGG 0.575752 3.1:-64287081 None:intergenic
GATGAATGAAGGTGAAGGAG+GGG 0.576275 3.1:+64286168 None:intergenic
AATCTTGATGGGATGAATGA+AGG 0.577942 3.1:+64286157 None:intergenic
GTGTGGGTTCAGCCTGGTAA+CGG 0.579940 3.1:+64287018 MS.gene70596:CDS
AGGAATTGATAAGGAAACTG+AGG 0.581256 3.1:+64286279 MS.gene70596:CDS
GAGGTTAAAGATGGTGAAAG+TGG 0.584687 3.1:+64286298 MS.gene70596:CDS
AATGCAGTATAGCACGTGTG+TGG 0.584778 3.1:+64287001 MS.gene70596:CDS
AAGATCTTAAAGATGTTGAT+GGG 0.585151 3.1:+64286821 MS.gene70596:CDS
AATTGTACCGTGAAAGGTGG+TGG 0.586653 3.1:+64287153 MS.gene70596:CDS
TGATGATGTGAGTAAGGAGG+AGG 0.588163 3.1:+64286417 MS.gene70596:CDS
GGTGAGCTTAATGTTGTCGA+GGG 0.589292 3.1:+64286442 MS.gene70596:CDS
GGAAGTTCAGAAGCTTGAGA+AGG 0.603977 3.1:+64286477 MS.gene70596:CDS
TAGAGAAAATGCGCTATGAG+AGG 0.604354 3.1:+64286863 MS.gene70596:CDS
ATGCAGTATAGCACGTGTGT+GGG 0.611437 3.1:+64287002 MS.gene70596:CDS
GATGTTAATTGTACCGTGAA+AGG 0.611552 3.1:+64287147 MS.gene70596:CDS
CAAACTCTCGAGCATTTCGT+CGG 0.612874 3.1:-64286788 None:intergenic
TAGCACGATGATCGAGCATG+GGG 0.649555 3.1:-64287080 None:intergenic
GATGTGAGTAAGGAGGAGGT+TGG 0.653294 3.1:+64286421 MS.gene70596:CDS
TGTCGAGGGTCCGAGTCAAG+AGG 0.658139 3.1:+64286456 MS.gene70596:CDS
TCTCTGAGACTAAGACATTG+GGG 0.658850 3.1:+64287118 MS.gene70596:CDS
AATGGAGCGTCTAAAGCAAG+AGG 0.669910 3.1:+64286912 MS.gene70596:CDS
GATGGGATGAATGAAGGTGA+AGG 0.675149 3.1:+64286163 None:intergenic
GTTAATTGTACCGTGAAAGG+TGG 0.683961 3.1:+64287150 MS.gene70596:CDS
GACGTGCCTATGGAACCGGA+AGG 0.727860 3.1:+64286974 MS.gene70596:CDS
AATACTGGCAAATCCACCAG+AGG 0.729786 3.1:+64286886 MS.gene70596:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGTTTAAAGGAATTGATA+AGG + chr3.1:64286270-64286289 MS.gene70596:CDS 20.0%
! AAAGATCTTAAAGATGTTGA+TGG + chr3.1:64286820-64286839 MS.gene70596:CDS 25.0%
! AACTAACAAGTAACAACAAA+AGG - chr3.1:64286547-64286566 None:intergenic 25.0%
! AAGATCTTAAAGATGTTGAT+GGG + chr3.1:64286821-64286840 MS.gene70596:CDS 25.0%
! ACTAACAAGTAACAACAAAA+GGG - chr3.1:64286546-64286565 None:intergenic 25.0%
!! ATTTTCTCTATTTCACTAAG+AGG - chr3.1:64286853-64286872 None:intergenic 25.0%
!!! AAGTTGATGATGAACAAAAA+TGG + chr3.1:64286206-64286225 MS.gene70596:CDS 25.0%
!!! TTAGTTTTAGACATTGTTGA+AGG + chr3.1:64286569-64286588 MS.gene70596:intron 25.0%
AAAATGGTGGTTAGAAGAAA+AGG + chr3.1:64286222-64286241 MS.gene70596:CDS 30.0%
AAATGGTGGTTAGAAGAAAA+GGG + chr3.1:64286223-64286242 MS.gene70596:CDS 30.0%
ATGAAACTGAATTGTTGTCA+AGG + chr3.1:64286686-64286705 MS.gene70596:intron 30.0%
ATTTGAACGTGAATGTTGAA+TGG + chr3.1:64286637-64286656 MS.gene70596:intron 30.0%
CTAACAAGTAACAACAAAAG+GGG - chr3.1:64286545-64286564 None:intergenic 30.0%
TTTAGACATTGTTGAAGGTT+TGG + chr3.1:64286574-64286593 MS.gene70596:intron 30.0%
!! TTGATGATGAACAAAAATGG+TGG + chr3.1:64286209-64286228 MS.gene70596:CDS 30.0%
!!! TGATGTTTTTGGTTTCAAAG+AGG + chr3.1:64286393-64286412 MS.gene70596:CDS 30.0%
AAGGAAACTGAGGTTAAAGA+TGG + chr3.1:64286289-64286308 MS.gene70596:CDS 35.0%
AATGTCTTAGTCTCAGAGAA+AGG - chr3.1:64287117-64287136 None:intergenic 35.0%
ACCATAAATTTACCGTTACC+AGG - chr3.1:64287033-64287052 None:intergenic 35.0%
AGGAATTGATAAGGAAACTG+AGG + chr3.1:64286279-64286298 MS.gene70596:CDS 35.0%
AGTGAGACAAGTAGATGATA+AGG + chr3.1:64286951-64286970 MS.gene70596:CDS 35.0%
GAATGTTGAATGGAATCGAA+AGG + chr3.1:64286647-64286666 MS.gene70596:intron 35.0%
GATGTTAATTGTACCGTGAA+AGG + chr3.1:64287147-64287166 MS.gene70596:CDS 35.0%
GTATCCTCTTTCAAATTCCA+AGG - chr3.1:64286370-64286389 None:intergenic 35.0%
GTGAGACAAGTAGATGATAA+GGG + chr3.1:64286952-64286971 MS.gene70596:CDS 35.0%
TGAACCTTGGAATTTGAAAG+AGG + chr3.1:64286363-64286382 MS.gene70596:CDS 35.0%
TGGAATTTGAAAGAGGATAC+TGG + chr3.1:64286370-64286389 MS.gene70596:CDS 35.0%
TTCTCTATTTCACTAAGAGG+TGG - chr3.1:64286850-64286869 None:intergenic 35.0%
! CAGATAATGTTGATGAACCT+TGG + chr3.1:64286350-64286369 MS.gene70596:CDS 35.0%
! TATACTGCATTTTCTCCTTC+CGG - chr3.1:64286992-64287011 None:intergenic 35.0%
!! GAAGAGGATGCTTTGTTTAA+AGG + chr3.1:64286259-64286278 MS.gene70596:CDS 35.0%
!! GGGTATTGATAATGAAGAAG+AGG + chr3.1:64286243-64286262 MS.gene70596:CDS 35.0%
!! TTCTCTGAGACTAAGACATT+GGG + chr3.1:64287117-64287136 MS.gene70596:CDS 35.0%
!! TTTCTCTGAGACTAAGACAT+TGG + chr3.1:64287116-64287135 MS.gene70596:CDS 35.0%
GAGGTTAAAGATGGTGAAAG+TGG + chr3.1:64286298-64286317 MS.gene70596:CDS 40.0%
GTTAATTGTACCGTGAAAGG+TGG + chr3.1:64287150-64287169 MS.gene70596:CDS 40.0%
TAGAGAAAATGCGCTATGAG+AGG + chr3.1:64286863-64286882 MS.gene70596:CDS 40.0%
TAGTCTCAGAGAAAGGTCTA+AGG - chr3.1:64287110-64287129 None:intergenic 40.0%
TATCTGCTCCAACACCAATA+TGG - chr3.1:64286336-64286355 None:intergenic 40.0%
TTCTGAACTTCCTCTTGACT+CGG - chr3.1:64286469-64286488 None:intergenic 40.0%
! AGAGGATGATGATGTGAGTA+AGG + chr3.1:64286411-64286430 MS.gene70596:CDS 40.0%
! GATGGTGAAAGTGGTGATTT+TGG + chr3.1:64286307-64286326 MS.gene70596:CDS 40.0%
! TTTGGTGACCATATTGGTGT+TGG + chr3.1:64286325-64286344 MS.gene70596:CDS 40.0%
!! GAGGATACTGGTGATGTTTT+TGG + chr3.1:64286382-64286401 MS.gene70596:CDS 40.0%
!! GCCTGGTAACGGTAAATTTA+TGG + chr3.1:64287029-64287048 MS.gene70596:CDS 40.0%
!! GGTGATTTTGGTGACCATAT+TGG + chr3.1:64286319-64286338 MS.gene70596:CDS 40.0%
!! TCTCTGAGACTAAGACATTG+GGG + chr3.1:64287118-64287137 MS.gene70596:CDS 40.0%
!! TTTTGCTGTATTGTGACTGC+AGG + chr3.1:64286724-64286743 MS.gene70596:intron 40.0%
AATACTGGCAAATCCACCAG+AGG + chr3.1:64286886-64286905 MS.gene70596:CDS 45.0%
AATGCAGTATAGCACGTGTG+TGG + chr3.1:64287001-64287020 MS.gene70596:CDS 45.0%
AATTGTACCGTGAAAGGTGG+TGG + chr3.1:64287153-64287172 MS.gene70596:CDS 45.0%
ATGCAGTATAGCACGTGTGT+GGG + chr3.1:64287002-64287021 MS.gene70596:CDS 45.0%
ATGCGCTATGAGAGGAATAC+TGG + chr3.1:64286871-64286890 MS.gene70596:CDS 45.0%
CAAACTCTCGAGCATTTCGT+CGG - chr3.1:64286791-64286810 None:intergenic 45.0%
CAAATCCACCAGAGGTGAAA+TGG + chr3.1:64286894-64286913 MS.gene70596:CDS 45.0%
GGAAGTTCAGAAGCTTGAGA+AGG + chr3.1:64286477-64286496 MS.gene70596:CDS 45.0%
GGTGAGCTTAATGTTGTCGA+GGG + chr3.1:64286442-64286461 MS.gene70596:CDS 45.0%
TGAAACACCACCACCTTTCA+CGG - chr3.1:64287163-64287182 None:intergenic 45.0%
TGGTGAGCTTAATGTTGTCG+AGG + chr3.1:64286441-64286460 MS.gene70596:CDS 45.0%
! AATGGAGCGTCTAAAGCAAG+AGG + chr3.1:64286912-64286931 MS.gene70596:CDS 45.0%
! ACTGCAGGAACGAAAGCTTT+TGG + chr3.1:64286739-64286758 MS.gene70596:intron 45.0%
! CATTGCTGTTCTCGAAGGTT+TGG + chr3.1:64286513-64286532 MS.gene70596:intron 45.0%
! GGATGATGATGTGAGTAAGG+AGG + chr3.1:64286414-64286433 MS.gene70596:CDS 45.0%
! TGATGATGTGAGTAAGGAGG+AGG + chr3.1:64286417-64286436 MS.gene70596:CDS 45.0%
! TTTTCTCCTTCCGGTTCCAT+AGG - chr3.1:64286983-64287002 None:intergenic 45.0%
AGACTAAGACATTGGGGCTC+TGG + chr3.1:64287124-64287143 MS.gene70596:CDS 50.0%
AGTAGCACGATGATCGAGCA+TGG - chr3.1:64287085-64287104 None:intergenic 50.0%
GACTAAGACATTGGGGCTCT+GGG + chr3.1:64287125-64287144 MS.gene70596:CDS 50.0%
GATGATAAGGGACGTGCCTA+TGG + chr3.1:64286964-64286983 MS.gene70596:CDS 50.0%
GATGTGAGTAAGGAGGAGGT+TGG + chr3.1:64286421-64286440 MS.gene70596:CDS 50.0%
GTAGCACGATGATCGAGCAT+GGG - chr3.1:64287084-64287103 None:intergenic 50.0%
TAGACGCTCCATTTCACCTC+TGG - chr3.1:64286905-64286924 None:intergenic 50.0%
TAGCACGATGATCGAGCATG+GGG - chr3.1:64287083-64287102 None:intergenic 50.0%
! GAGCTCATTGCTGTTCTCGA+AGG + chr3.1:64286508-64286527 MS.gene70596:CDS 50.0%
! GCGTCTAAAGCAAGAGGAAG+CGG + chr3.1:64286918-64286937 MS.gene70596:CDS 50.0%
! GGTGACTTGATTGCTGCTTC+TGG + chr3.1:64286760-64286779 MS.gene70596:CDS 50.0%
! GTGAAAGGTGGTGGTGTTTC+AGG + chr3.1:64287162-64287181 MS.gene70596:CDS 50.0%
AAGGGACGTGCCTATGGAAC+CGG + chr3.1:64286970-64286989 MS.gene70596:CDS 55.0%
ACGCTCCATTTCACCTCTGG+TGG - chr3.1:64286902-64286921 None:intergenic 55.0%
GTGTGGGTTCAGCCTGGTAA+CGG + chr3.1:64287018-64287037 MS.gene70596:CDS 55.0%
AGGAGGGGAGAGAGTGAGGA+AGG + chr3.1:64286183-64286202 MS.gene70596:CDS 60.0%
GACGTGCCTATGGAACCGGA+AGG + chr3.1:64286974-64286993 MS.gene70596:CDS 60.0%
GTGAAGGAGGGGAGAGAGTG+AGG + chr3.1:64286179-64286198 MS.gene70596:CDS 60.0%
TGTCGAGGGTCCGAGTCAAG+AGG + chr3.1:64286456-64286475 MS.gene70596:CDS 60.0%
GCACGTGTGTGGGTTCAGCC+TGG + chr3.1:64287012-64287031 MS.gene70596:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 64286169 64287203 64286169 ID=MS.gene70596
chr3.1 mRNA 64286169 64287203 64286169 ID=MS.gene70596.t1;Parent=MS.gene70596
chr3.1 exon 64286169 64286529 64286169 ID=MS.gene70596.t1.exon1;Parent=MS.gene70596.t1
chr3.1 CDS 64286169 64286529 64286169 ID=cds.MS.gene70596.t1;Parent=MS.gene70596.t1
chr3.1 exon 64286746 64287203 64286746 ID=MS.gene70596.t1.exon2;Parent=MS.gene70596.t1
chr3.1 CDS 64286746 64287203 64286746 ID=cds.MS.gene70596.t1;Parent=MS.gene70596.t1
Gene Sequence

>MS.gene70596

ATGAATGAAGGTGAAGGAGGGGAGAGAGTGAGGAAGGAAGTTGATGATGAACAAAAATGGTGGTTAGAAGAAAAGGGTATTGATAATGAAGAAGAGGATGCTTTGTTTAAAGGAATTGATAAGGAAACTGAGGTTAAAGATGGTGAAAGTGGTGATTTTGGTGACCATATTGGTGTTGGAGCAGATAATGTTGATGAACCTTGGAATTTGAAAGAGGATACTGGTGATGTTTTTGGTTTCAAAGAGGATGATGATGTGAGTAAGGAGGAGGTTGGTGAGCTTAATGTTGTCGAGGGTCCGAGTCAAGAGGAAGTTCAGAAGCTTGAGAAGGAAGAGAAAGAGCTCATTGCTGTTCTCGAAGGTTTGGCTTAAACCCCTTTTGTTGTTACTTGTTAGTTTGTTAGTTTTAGACATTGTTGAAGGTTTGGTGTAACGAATTGATGTACTGTTGAAGATTTTCTAGATATAATTTGAACGTGAATGTTGAATGGAATCGAAAGGTGTTGCGATATATTGAATGAAACTGAATTGTTGTCAAGGTGAGTGATGTCATGTTTTTGCTGTATTGTGACTGCAGGAACGAAAGCTTTTGGTGACTTGATTGCTGCTTCTGGAATCACCGACGAAATGCTCGAGAGTTTGATTGCGTTGAAAGATCTTAAAGATGTTGATGGGTTGCCACCTCTTAGTGAAATAGAGAAAATGCGCTATGAGAGGAATACTGGCAAATCCACCAGAGGTGAAATGGAGCGTCTAAAGCAAGAGGAAGCGGCGAAAGCTAGAGTGAGACAAGTAGATGATAAGGGACGTGCCTATGGAACCGGAAGGAGAAAATGCAGTATAGCACGTGTGTGGGTTCAGCCTGGTAACGGTAAATTTATGGTTAACGATAAAGAGTTTGACGTCTATTTCCCCATGCTCGATCATCGTGCTACTCTCCTTAGACCTTTCTCTGAGACTAAGACATTGGGGCTCTGGGATGTTAATTGTACCGTGAAAGGTGGTGGTGTTTCAGGTATAGTTAATGACTTTTAA

Protein sequence

>MS.gene70596.t1

MNEGEGGERVRKEVDDEQKWWLEEKGIDNEEEDALFKGIDKETEVKDGESGDFGDHIGVGADNVDEPWNLKEDTGDVFGFKEDDDVSKEEVGELNVVEGPSQEEVQKLEKEEKELIAVLEGTKAFGDLIAASGITDEMLESLIALKDLKDVDGLPPLSEIEKMRYERNTGKSTRGEMERLKQEEAAKARVRQVDDKGRAYGTGRRKCSIARVWVQPGNGKFMVNDKEFDVYFPMLDHRATLLRPFSETKTLGLWDVNCTVKGGGVSGIVNDF