Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75761.t1 | XP_003603595.1 | 85.8 | 261 | 12 | 1 | 1 | 236 | 452 | 712 | 5.10E-116 | 427.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75761.t1 | Q93YU8 | 25.5 | 243 | 147 | 6 | 4 | 214 | 538 | 778 | 7.1e-10 | 65.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75761.t1 | G7J2X7 | 85.8 | 261 | 12 | 1 | 1 | 236 | 452 | 712 | 3.6e-116 | 427.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049298 | MS.gene75761 | 0.806077 | 9.80E-50 | -1.69E-46 |
| MS.gene050667 | MS.gene75761 | 0.805388 | 1.37E-49 | -1.69E-46 |
| MS.gene050874 | MS.gene75761 | 0.803859 | 2.85E-49 | -1.69E-46 |
| MS.gene050875 | MS.gene75761 | 0.834984 | 2.18E-56 | -1.69E-46 |
| MS.gene051025 | MS.gene75761 | 0.833719 | 4.54E-56 | -1.69E-46 |
| MS.gene051914 | MS.gene75761 | 0.860221 | 2.40E-63 | -1.69E-46 |
| MS.gene052169 | MS.gene75761 | 0.816877 | 4.41E-52 | -1.69E-46 |
| MS.gene052209 | MS.gene75761 | 0.802099 | 6.59E-49 | -1.69E-46 |
| MS.gene052308 | MS.gene75761 | 0.828881 | 7.03E-55 | -1.69E-46 |
| MS.gene052367 | MS.gene75761 | 0.81102 | 8.63E-51 | -1.69E-46 |
| MS.gene053108 | MS.gene75761 | 0.803926 | 2.76E-49 | -1.69E-46 |
| MS.gene053173 | MS.gene75761 | 0.854304 | 1.35E-61 | -1.69E-46 |
| MS.gene053348 | MS.gene75761 | 0.823748 | 1.17E-53 | -1.69E-46 |
| MS.gene054459 | MS.gene75761 | 0.836129 | 1.12E-56 | -1.69E-46 |
| MS.gene054551 | MS.gene75761 | 0.849506 | 3.10E-60 | -1.69E-46 |
| MS.gene054593 | MS.gene75761 | 0.815219 | 1.03E-51 | -1.69E-46 |
| MS.gene054633 | MS.gene75761 | 0.834039 | 3.77E-56 | -1.69E-46 |
| MS.gene054662 | MS.gene75761 | 0.881065 | 3.27E-70 | -1.69E-46 |
| MS.gene054941 | MS.gene75761 | 0.812533 | 4.04E-51 | -1.69E-46 |
| MS.gene055308 | MS.gene75761 | 0.816651 | 4.96E-52 | -1.69E-46 |
| MS.gene055578 | MS.gene75761 | 0.815007 | 1.15E-51 | -1.69E-46 |
| MS.gene055616 | MS.gene75761 | 0.802551 | 5.32E-49 | -1.69E-46 |
| MS.gene055982 | MS.gene75761 | 0.802068 | 6.69E-49 | -1.69E-46 |
| MS.gene056041 | MS.gene75761 | 0.819198 | 1.32E-52 | -1.69E-46 |
| MS.gene05640 | MS.gene75761 | 0.863678 | 2.10E-64 | -1.69E-46 |
| MS.gene05652 | MS.gene75761 | 0.836075 | 1.16E-56 | -1.69E-46 |
| MS.gene056618 | MS.gene75761 | 0.822677 | 2.08E-53 | -1.69E-46 |
| MS.gene057101 | MS.gene75761 | 0.808058 | 3.73E-50 | -1.69E-46 |
| MS.gene057367 | MS.gene75761 | 0.875203 | 3.71E-68 | -1.69E-46 |
| MS.gene057458 | MS.gene75761 | 0.808534 | 2.95E-50 | -1.69E-46 |
| MS.gene057669 | MS.gene75761 | 0.824848 | 6.46E-54 | -1.69E-46 |
| MS.gene058128 | MS.gene75761 | 0.840815 | 6.93E-58 | -1.69E-46 |
| MS.gene058853 | MS.gene75761 | 0.834224 | 3.39E-56 | -1.69E-46 |
| MS.gene059553 | MS.gene75761 | 0.82325 | 1.53E-53 | -1.69E-46 |
| MS.gene059616 | MS.gene75761 | 0.805838 | 1.10E-49 | -1.69E-46 |
| MS.gene060275 | MS.gene75761 | 0.819386 | 1.19E-52 | -1.69E-46 |
| MS.gene060291 | MS.gene75761 | 0.830284 | 3.20E-55 | -1.69E-46 |
| MS.gene06093 | MS.gene75761 | 0.894299 | 2.81E-75 | -1.69E-46 |
| MS.gene061260 | MS.gene75761 | 0.802677 | 5.01E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 43 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGCTTGGATTGGTGGATTT+TGG | 0.116448 | 3.3:-90038387 | None:intergenic |
| CCCTTCGTTGTAAATGCTTC+TGG | 0.304621 | 3.3:-90038597 | None:intergenic |
| GGAGGAAGAGATGAAGTTAA+AGG | 0.341872 | 3.3:+90038513 | MS.gene75761:CDS |
| GAACATATTCTCCCACATAT+TGG | 0.386881 | 3.3:-90038108 | None:intergenic |
| GCAGATTTCGCTAGCTCGTC+AGG | 0.404088 | 3.3:-90038445 | None:intergenic |
| ACGACCGCACCGTGCAGCTT+TGG | 0.406384 | 3.3:+90038219 | MS.gene75761:CDS |
| TGATATTTCTCAAGATCATA+AGG | 0.409383 | 3.3:+90038178 | MS.gene75761:CDS |
| GAGAAATATCAAGAGATTTG+AGG | 0.419497 | 3.3:-90038166 | None:intergenic |
| TGAGCTCTTCCGCGCGCTAT+CGG | 0.425047 | 3.3:+90038789 | MS.gene75761:CDS |
| ATCATCTCTTTCTAGATCAT+CGG | 0.445892 | 3.3:-90038623 | None:intergenic |
| GGTACCATTCTTCAAAAGCT+TGG | 0.451334 | 3.3:-90038576 | None:intergenic |
| CTTTCAGGATGGCCAATATG+TGG | 0.463738 | 3.3:+90038096 | None:intergenic |
| GATAGCGCGCGGAAGAGCTC+AGG | 0.466453 | 3.3:-90038787 | None:intergenic |
| ACAAACTGCTTCTCCGAGAT+CGG | 0.469741 | 3.3:-90038661 | None:intergenic |
| TCATCTCTTTCTAGATCATC+GGG | 0.475608 | 3.3:-90038622 | None:intergenic |
| TAAAGAAGGGCTTGGATTGG+TGG | 0.484719 | 3.3:-90038394 | None:intergenic |
| GAAATGTGAGGAAACTAGAA+AGG | 0.486729 | 3.3:+90038537 | MS.gene75761:CDS |
| ATTGCCCACGCTCAAGGTGG+TGG | 0.492952 | 3.3:+90038856 | MS.gene75761:CDS |
| AATCATGCCATGCATAAAGA+AGG | 0.514400 | 3.3:-90038408 | None:intergenic |
| AAAGACGATCATACTTCAGC+AGG | 0.518195 | 3.3:+90038492 | MS.gene75761:CDS |
| TCCAAGCCCTTCTTTATGCA+TGG | 0.533581 | 3.3:+90038401 | MS.gene75761:CDS |
| AAGTCGATTGCCCACGCTCA+AGG | 0.535916 | 3.3:+90038850 | MS.gene75761:CDS |
| GCCATGCATAAAGAAGGGCT+TGG | 0.543280 | 3.3:-90038402 | None:intergenic |
| CTTTATCACTGCAGAAAATG+AGG | 0.543457 | 3.3:-90038473 | None:intergenic |
| CCAGAAGCATTTACAACGAA+GGG | 0.551600 | 3.3:+90038597 | MS.gene75761:CDS |
| GCATAAAGAAGGGCTTGGAT+TGG | 0.556125 | 3.3:-90038397 | None:intergenic |
| AAATACAAACAATCCGATCT+CGG | 0.564703 | 3.3:+90038648 | MS.gene75761:CDS |
| TTTCAGGATGGCCAATATGT+GGG | 0.579247 | 3.3:+90038097 | None:intergenic |
| CGCACCACCACCTTGAGCGT+GGG | 0.587071 | 3.3:-90038860 | None:intergenic |
| CCGCACCACCACCTTGAGCG+TGG | 0.589202 | 3.3:-90038861 | None:intergenic |
| CAGAAGCATTTACAACGAAG+GGG | 0.593582 | 3.3:+90038598 | MS.gene75761:CDS |
| GGTGCGGTCGTAATGATGCT+TGG | 0.594455 | 3.3:-90038207 | None:intergenic |
| CCACGCTCAAGGTGGTGGTG+CGG | 0.602808 | 3.3:+90038861 | MS.gene75761:CDS |
| ATCATGCCATGCATAAAGAA+GGG | 0.611365 | 3.3:-90038407 | None:intergenic |
| GAAGTTAAAGGAGAAATGTG+AGG | 0.611782 | 3.3:+90038525 | MS.gene75761:CDS |
| AAACTAGAAAGGAATTCTTG+AGG | 0.618185 | 3.3:+90038548 | MS.gene75761:CDS |
| ACCAGAAGCATTTACAACGA+AGG | 0.625698 | 3.3:+90038596 | MS.gene75761:CDS |
| GACGATCATACTTCAGCAGG+AGG | 0.629306 | 3.3:+90038495 | MS.gene75761:CDS |
| GATTACATTCCAAAGCTGCA+CGG | 0.636378 | 3.3:-90038228 | None:intergenic |
| CATTCCAAAGCTGCACGGTG+CGG | 0.678361 | 3.3:-90038223 | None:intergenic |
| GAGTATAATCCGATAGCGCG+CGG | 0.690717 | 3.3:-90038798 | None:intergenic |
| AGAAGCATTTACAACGAAGG+GGG | 0.702303 | 3.3:+90038599 | MS.gene75761:CDS |
| TCGATTGCCCACGCTCAAGG+TGG | 0.713047 | 3.3:+90038853 | MS.gene75761:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGATATTTCTCAAGATCATA+AGG | + | chr3.3:90038178-90038197 | MS.gene75761:CDS | 25.0% |
| AAACTAGAAAGGAATTCTTG+AGG | + | chr3.3:90038548-90038567 | MS.gene75761:CDS | 30.0% | |
| AAATACAAACAATCCGATCT+CGG | + | chr3.3:90038648-90038667 | MS.gene75761:CDS | 30.0% | |
| ATCATCTCTTTCTAGATCAT+CGG | - | chr3.3:90038626-90038645 | None:intergenic | 30.0% | |
| GAGAAATATCAAGAGATTTG+AGG | - | chr3.3:90038169-90038188 | None:intergenic | 30.0% | |
| TTGGAATGTAATCCAAGAAT+GGG | + | chr3.3:90038238-90038257 | MS.gene75761:intron | 30.0% | |
| ! | TTTGGAATGTAATCCAAGAA+TGG | + | chr3.3:90038237-90038256 | MS.gene75761:intron | 30.0% |
| !! | AAAACCAAGCTTTTGAAGAA+TGG | + | chr3.3:90038572-90038591 | MS.gene75761:CDS | 30.0% |
| !!! | GAGACGAGATTTTTTCTTTT+AGG | - | chr3.3:90038357-90038376 | None:intergenic | 30.0% |
| AATCATGCCATGCATAAAGA+AGG | - | chr3.3:90038411-90038430 | None:intergenic | 35.0% | |
| ATCATGCCATGCATAAAGAA+GGG | - | chr3.3:90038410-90038429 | None:intergenic | 35.0% | |
| CTTTATCACTGCAGAAAATG+AGG | - | chr3.3:90038476-90038495 | None:intergenic | 35.0% | |
| GAAATGTGAGGAAACTAGAA+AGG | + | chr3.3:90038537-90038556 | MS.gene75761:CDS | 35.0% | |
| GAACATATTCTCCCACATAT+TGG | - | chr3.3:90038111-90038130 | None:intergenic | 35.0% | |
| GAAGTTAAAGGAGAAATGTG+AGG | + | chr3.3:90038525-90038544 | MS.gene75761:CDS | 35.0% | |
| TCATCTCTTTCTAGATCATC+GGG | - | chr3.3:90038625-90038644 | None:intergenic | 35.0% | |
| !!! | TTCTTTTAGGTTGCTTTCGA+TGG | - | chr3.3:90038344-90038363 | None:intergenic | 35.0% |
| AAAGACGATCATACTTCAGC+AGG | + | chr3.3:90038492-90038511 | MS.gene75761:CDS | 40.0% | |
| ACATCCAAGCTCTTTATAGC+TGG | + | chr3.3:90038300-90038319 | MS.gene75761:intron | 40.0% | |
| CAAATTGTGACTCCCATTCT+TGG | - | chr3.3:90038253-90038272 | None:intergenic | 40.0% | |
| GATTACATTCCAAAGCTGCA+CGG | - | chr3.3:90038231-90038250 | None:intergenic | 40.0% | |
| GGAGGAAGAGATGAAGTTAA+AGG | + | chr3.3:90038513-90038532 | MS.gene75761:CDS | 40.0% | |
| GGTACCATTCTTCAAAAGCT+TGG | - | chr3.3:90038579-90038598 | None:intergenic | 40.0% | |
| ! | GGTCTGTAACTATTTTCAGC+TGG | - | chr3.3:90038148-90038167 | None:intergenic | 40.0% |
| !! | ACCAGAAGCATTTACAACGA+AGG | + | chr3.3:90038596-90038615 | MS.gene75761:CDS | 40.0% |
| !! | AGAAGCATTTACAACGAAGG+GGG | + | chr3.3:90038599-90038618 | MS.gene75761:CDS | 40.0% |
| !! | CAGAAGCATTTACAACGAAG+GGG | + | chr3.3:90038598-90038617 | MS.gene75761:CDS | 40.0% |
| !! | CCAGAAGCATTTACAACGAA+GGG | + | chr3.3:90038597-90038616 | MS.gene75761:CDS | 40.0% |
| ACAAACTGCTTCTCCGAGAT+CGG | - | chr3.3:90038664-90038683 | None:intergenic | 45.0% | |
| CCCTTCGTTGTAAATGCTTC+TGG | - | chr3.3:90038600-90038619 | None:intergenic | 45.0% | |
| GCATAAAGAAGGGCTTGGAT+TGG | - | chr3.3:90038400-90038419 | None:intergenic | 45.0% | |
| TCCAAGCCCTTCTTTATGCA+TGG | + | chr3.3:90038401-90038420 | MS.gene75761:CDS | 45.0% | |
| TGAGCCAGCTATAAAGAGCT+TGG | - | chr3.3:90038307-90038326 | None:intergenic | 45.0% | |
| ! | TAAAGAAGGGCTTGGATTGG+TGG | - | chr3.3:90038397-90038416 | None:intergenic | 45.0% |
| !!! | ATTGGTGGATTTTGGGCTCT+TGG | - | chr3.3:90038382-90038401 | None:intergenic | 45.0% |
| !!! | GGCTTGGATTGGTGGATTTT+GGG | - | chr3.3:90038389-90038408 | None:intergenic | 45.0% |
| GACGATCATACTTCAGCAGG+AGG | + | chr3.3:90038495-90038514 | MS.gene75761:CDS | 50.0% | |
| GAGTATAATCCGATAGCGCG+CGG | - | chr3.3:90038801-90038820 | None:intergenic | 50.0% | |
| GCCATGCATAAAGAAGGGCT+TGG | - | chr3.3:90038405-90038424 | None:intergenic | 50.0% | |
| !!! | GCAAGCGAGTTTTGAGACTC+TGG | - | chr3.3:90038768-90038787 | None:intergenic | 50.0% |
| !!! | GGGCTTGGATTGGTGGATTT+TGG | - | chr3.3:90038390-90038409 | None:intergenic | 50.0% |
| AAGTCGATTGCCCACGCTCA+AGG | + | chr3.3:90038850-90038869 | MS.gene75761:CDS | 55.0% | |
| CATTCCAAAGCTGCACGGTG+CGG | - | chr3.3:90038226-90038245 | None:intergenic | 55.0% | |
| GCAGATTTCGCTAGCTCGTC+AGG | - | chr3.3:90038448-90038467 | None:intergenic | 55.0% | |
| GGTGCGGTCGTAATGATGCT+TGG | - | chr3.3:90038210-90038229 | None:intergenic | 55.0% | |
| !! | GGTGGATTTTGGGCTCTTGG+CGG | - | chr3.3:90038379-90038398 | None:intergenic | 55.0% |
| ATTGCCCACGCTCAAGGTGG+TGG | + | chr3.3:90038856-90038875 | MS.gene75761:CDS | 60.0% | |
| TCGATTGCCCACGCTCAAGG+TGG | + | chr3.3:90038853-90038872 | MS.gene75761:CDS | 60.0% | |
| TGAGCTCTTCCGCGCGCTAT+CGG | + | chr3.3:90038789-90038808 | MS.gene75761:CDS | 60.0% | |
| ACGACCGCACCGTGCAGCTT+TGG | + | chr3.3:90038219-90038238 | MS.gene75761:CDS | 65.0% | |
| CGCACCACCACCTTGAGCGT+GGG | - | chr3.3:90038863-90038882 | None:intergenic | 65.0% | |
| GATAGCGCGCGGAAGAGCTC+AGG | - | chr3.3:90038790-90038809 | None:intergenic | 65.0% | |
| ! | CCACGCTCAAGGTGGTGGTG+CGG | + | chr3.3:90038861-90038880 | MS.gene75761:CDS | 65.0% |
| CCGCACCACCACCTTGAGCG+TGG | - | chr3.3:90038864-90038883 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 90038103 | 90038888 | 90038103 | ID=MS.gene75761 |
| chr3.3 | mRNA | 90038103 | 90038888 | 90038103 | ID=MS.gene75761.t1;Parent=MS.gene75761 |
| chr3.3 | exon | 90038103 | 90038244 | 90038103 | ID=MS.gene75761.t1.exon1;Parent=MS.gene75761.t1 |
| chr3.3 | CDS | 90038103 | 90038244 | 90038103 | ID=cds.MS.gene75761.t1;Parent=MS.gene75761.t1 |
| chr3.3 | exon | 90038319 | 90038888 | 90038319 | ID=MS.gene75761.t1.exon2;Parent=MS.gene75761.t1 |
| chr3.3 | CDS | 90038319 | 90038888 | 90038319 | ID=cds.MS.gene75761.t1;Parent=MS.gene75761.t1 |
| Gene Sequence |
| Protein sequence |
