Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80558.t1 | XP_003609142.1 | 98.1 | 154 | 3 | 0 | 1 | 154 | 770 | 923 | 1.60E-73 | 285.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80558.t1 | P57792 | 82.4 | 148 | 26 | 0 | 7 | 154 | 776 | 923 | 9.9e-61 | 234.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80558.t1 | G7JT17 | 98.1 | 154 | 3 | 0 | 1 | 154 | 770 | 923 | 1.1e-73 | 285.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene80558 | 0.871531 | 6.37E-67 | -1.69E-46 |
| MS.gene049983 | MS.gene80558 | 0.84562 | 3.63E-59 | -1.69E-46 |
| MS.gene04998 | MS.gene80558 | 0.804224 | 2.39E-49 | -1.69E-46 |
| MS.gene050359 | MS.gene80558 | 0.804511 | 2.09E-49 | -1.69E-46 |
| MS.gene050414 | MS.gene80558 | 0.819923 | 9.00E-53 | -1.69E-46 |
| MS.gene050646 | MS.gene80558 | 0.807232 | 5.59E-50 | -1.69E-46 |
| MS.gene051821 | MS.gene80558 | 0.820113 | 8.14E-53 | -1.69E-46 |
| MS.gene051823 | MS.gene80558 | 0.807346 | 5.29E-50 | -1.69E-46 |
| MS.gene052118 | MS.gene80558 | 0.824563 | 7.54E-54 | -1.69E-46 |
| MS.gene052533 | MS.gene80558 | 0.811001 | 8.71E-51 | -1.69E-46 |
| MS.gene054489 | MS.gene80558 | 0.810283 | 1.25E-50 | -1.69E-46 |
| MS.gene054892 | MS.gene80558 | 0.87682 | 1.03E-68 | -1.69E-46 |
| MS.gene054893 | MS.gene80558 | 0.876033 | 1.92E-68 | -1.69E-46 |
| MS.gene054957 | MS.gene80558 | 0.829609 | 4.68E-55 | -1.69E-46 |
| MS.gene055231 | MS.gene80558 | 0.822659 | 2.10E-53 | -1.69E-46 |
| MS.gene05615 | MS.gene80558 | 0.808128 | 3.61E-50 | -1.69E-46 |
| MS.gene05624 | MS.gene80558 | 0.826585 | 2.50E-54 | -1.69E-46 |
| MS.gene056677 | MS.gene80558 | 0.807185 | 5.72E-50 | -1.69E-46 |
| MS.gene056699 | MS.gene80558 | 0.806853 | 6.72E-50 | -1.69E-46 |
| MS.gene05686 | MS.gene80558 | 0.806016 | 1.01E-49 | -1.69E-46 |
| MS.gene057174 | MS.gene80558 | 0.809685 | 1.67E-50 | -1.69E-46 |
| MS.gene057622 | MS.gene80558 | 0.857094 | 2.06E-62 | -1.69E-46 |
| MS.gene058095 | MS.gene80558 | 0.867849 | 1.01E-65 | -1.69E-46 |
| MS.gene058159 | MS.gene80558 | 0.805841 | 1.10E-49 | -1.69E-46 |
| MS.gene058429 | MS.gene80558 | 0.806053 | 9.91E-50 | -1.69E-46 |
| MS.gene058592 | MS.gene80558 | 0.818549 | 1.85E-52 | -1.69E-46 |
| MS.gene05878 | MS.gene80558 | 0.830039 | 3.68E-55 | -1.69E-46 |
| MS.gene059170 | MS.gene80558 | 0.811549 | 6.62E-51 | -1.69E-46 |
| MS.gene05952 | MS.gene80558 | 0.85383 | 1.84E-61 | -1.69E-46 |
| MS.gene05956 | MS.gene80558 | 0.824683 | 7.07E-54 | -1.69E-46 |
| MS.gene059686 | MS.gene80558 | 0.811436 | 7.01E-51 | -1.69E-46 |
| MS.gene05985 | MS.gene80558 | -0.816051 | 6.75E-52 | -1.69E-46 |
| MS.gene059901 | MS.gene80558 | -0.801533 | 8.61E-49 | -1.69E-46 |
| MS.gene06023 | MS.gene80558 | 0.814192 | 1.75E-51 | -1.69E-46 |
| MS.gene060413 | MS.gene80558 | 0.802341 | 5.87E-49 | -1.69E-46 |
| MS.gene060522 | MS.gene80558 | 0.8351 | 2.04E-56 | -1.69E-46 |
| MS.gene060840 | MS.gene80558 | 0.801073 | 1.07E-48 | -1.69E-46 |
| MS.gene060932 | MS.gene80558 | 0.802376 | 5.78E-49 | -1.69E-46 |
| MS.gene060933 | MS.gene80558 | 0.815169 | 1.06E-51 | -1.69E-46 |
| MS.gene060937 | MS.gene80558 | 0.814601 | 1.42E-51 | -1.69E-46 |
| MS.gene061100 | MS.gene80558 | 0.818605 | 1.80E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80558.t1 | MTR_4g112430 | 98.052 | 154 | 3 | 0 | 1 | 154 | 770 | 923 | 5.11e-99 | 309 |
| MS.gene80558.t1 | MTR_8g069920 | 79.739 | 153 | 30 | 1 | 3 | 154 | 784 | 936 | 3.20e-74 | 240 |
| MS.gene80558.t1 | MTR_3g037330 | 77.124 | 153 | 34 | 1 | 3 | 154 | 693 | 845 | 7.33e-73 | 236 |
| MS.gene80558.t1 | MTR_3g037330 | 77.124 | 153 | 34 | 1 | 3 | 154 | 763 | 915 | 8.88e-73 | 236 |
| MS.gene80558.t1 | MTR_0021s0360 | 76.471 | 153 | 35 | 1 | 3 | 154 | 764 | 916 | 1.62e-72 | 235 |
| MS.gene80558.t1 | MTR_2g099690 | 71.724 | 145 | 41 | 0 | 10 | 154 | 796 | 940 | 2.85e-68 | 223 |
| MS.gene80558.t1 | MTR_8g018290 | 69.595 | 148 | 45 | 0 | 7 | 154 | 447 | 594 | 9.67e-67 | 217 |
| MS.gene80558.t1 | MTR_8g018290 | 71.034 | 145 | 42 | 0 | 10 | 154 | 457 | 601 | 1.14e-66 | 217 |
| MS.gene80558.t1 | MTR_6g006910 | 62.658 | 158 | 53 | 1 | 3 | 154 | 775 | 932 | 5.14e-61 | 203 |
| MS.gene80558.t1 | MTR_2g099690 | 70.000 | 110 | 33 | 0 | 10 | 119 | 796 | 905 | 3.63e-45 | 157 |
| MS.gene80558.t1 | MTR_5g037210 | 60.163 | 123 | 49 | 0 | 29 | 151 | 770 | 892 | 6.06e-44 | 154 |
| MS.gene80558.t1 | MTR_7g073480 | 58.974 | 117 | 48 | 0 | 35 | 151 | 926 | 1042 | 4.69e-43 | 151 |
| MS.gene80558.t1 | MTR_3g007240 | 50.350 | 143 | 71 | 0 | 12 | 154 | 828 | 970 | 8.17e-40 | 142 |
| MS.gene80558.t1 | MTR_8g044210 | 57.377 | 122 | 52 | 0 | 33 | 154 | 814 | 935 | 4.70e-39 | 140 |
| MS.gene80558.t1 | MTR_2g090610 | 47.059 | 119 | 61 | 2 | 36 | 154 | 693 | 809 | 5.18e-30 | 114 |
| MS.gene80558.t1 | MTR_2g090610 | 47.059 | 119 | 61 | 2 | 36 | 154 | 693 | 809 | 5.51e-30 | 114 |
| MS.gene80558.t1 | MTR_2g099690 | 58.442 | 77 | 32 | 0 | 10 | 86 | 796 | 872 | 1.68e-22 | 93.2 |
| MS.gene80558.t1 | MTR_3g007240 | 41.573 | 89 | 52 | 0 | 12 | 100 | 828 | 916 | 2.88e-12 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80558.t1 | AT1G26130 | 80.921 | 152 | 29 | 0 | 3 | 154 | 773 | 924 | 8.87e-79 | 253 |
| MS.gene80558.t1 | AT1G26130 | 80.921 | 152 | 29 | 0 | 3 | 154 | 773 | 924 | 8.87e-79 | 253 |
| MS.gene80558.t1 | AT1G26130 | 80.921 | 152 | 29 | 0 | 3 | 154 | 773 | 924 | 8.87e-79 | 253 |
| MS.gene80558.t1 | AT1G26130 | 82.432 | 148 | 26 | 0 | 7 | 154 | 776 | 923 | 7.97e-78 | 250 |
| MS.gene80558.t1 | AT3G25610 | 78.808 | 151 | 32 | 0 | 4 | 154 | 772 | 922 | 9.71e-75 | 242 |
| MS.gene80558.t1 | AT1G13210 | 79.870 | 154 | 29 | 1 | 3 | 154 | 770 | 923 | 2.06e-74 | 241 |
| MS.gene80558.t1 | AT1G68710 | 76.974 | 152 | 33 | 1 | 3 | 154 | 778 | 927 | 4.39e-73 | 237 |
| MS.gene80558.t1 | AT1G68710 | 76.974 | 152 | 33 | 1 | 3 | 154 | 778 | 927 | 4.39e-73 | 237 |
| MS.gene80558.t1 | AT1G68710 | 76.974 | 152 | 33 | 1 | 3 | 154 | 778 | 927 | 4.39e-73 | 237 |
| MS.gene80558.t1 | AT1G54280 | 68.627 | 153 | 48 | 0 | 2 | 154 | 793 | 945 | 4.32e-67 | 220 |
| MS.gene80558.t1 | AT1G54280 | 68.627 | 153 | 48 | 0 | 2 | 154 | 793 | 945 | 4.32e-67 | 220 |
| MS.gene80558.t1 | AT1G54280 | 68.627 | 153 | 48 | 0 | 2 | 154 | 793 | 945 | 4.32e-67 | 220 |
| MS.gene80558.t1 | AT3G13900 | 68.243 | 148 | 47 | 0 | 7 | 154 | 796 | 943 | 9.52e-66 | 215 |
| MS.gene80558.t1 | AT3G13900 | 68.243 | 148 | 47 | 0 | 7 | 154 | 796 | 943 | 1.82e-65 | 216 |
| MS.gene80558.t1 | AT1G17500 | 66.667 | 153 | 51 | 0 | 2 | 154 | 772 | 924 | 6.31e-65 | 214 |
| MS.gene80558.t1 | AT1G17500 | 66.667 | 153 | 51 | 0 | 2 | 154 | 772 | 924 | 6.31e-65 | 214 |
| MS.gene80558.t1 | AT1G72700 | 66.892 | 148 | 49 | 0 | 7 | 154 | 789 | 936 | 8.80e-65 | 214 |
| MS.gene80558.t1 | AT1G72700 | 66.892 | 148 | 49 | 0 | 7 | 154 | 789 | 936 | 8.80e-65 | 214 |
| MS.gene80558.t1 | AT1G72700 | 66.892 | 148 | 49 | 0 | 7 | 154 | 789 | 936 | 8.80e-65 | 214 |
| MS.gene80558.t1 | AT3G27870 | 68.153 | 157 | 45 | 2 | 3 | 154 | 766 | 922 | 2.46e-64 | 212 |
| MS.gene80558.t1 | AT3G27870 | 68.153 | 157 | 45 | 2 | 3 | 154 | 575 | 731 | 2.48e-64 | 212 |
| MS.gene80558.t1 | AT3G27870 | 68.153 | 157 | 45 | 2 | 3 | 154 | 766 | 922 | 4.49e-64 | 212 |
| MS.gene80558.t1 | AT5G04930 | 53.623 | 138 | 63 | 1 | 17 | 154 | 780 | 916 | 1.85e-44 | 155 |
| MS.gene80558.t1 | AT3G13900 | 64.957 | 117 | 41 | 0 | 7 | 123 | 796 | 912 | 2.10e-44 | 155 |
| MS.gene80558.t1 | AT1G59820 | 62.069 | 116 | 44 | 0 | 36 | 151 | 784 | 899 | 1.95e-43 | 152 |
| MS.gene80558.t1 | AT5G44240 | 47.015 | 134 | 67 | 3 | 23 | 154 | 678 | 809 | 9.05e-31 | 116 |
| MS.gene80558.t1 | AT5G44240 | 47.015 | 134 | 67 | 3 | 23 | 154 | 710 | 841 | 1.03e-30 | 116 |
Find 26 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGATTCCAATATCTGCTTCT+TGG | 0.229651 | 8.1:+15480124 | None:intergenic |
| TCTGATTCTTCAGGCAATTA+AGG | 0.247624 | 8.1:-15480424 | MS.gene80558:intron |
| ATGCTCCAAGAAGCAGATAT+TGG | 0.386058 | 8.1:-15480129 | MS.gene80558:CDS |
| GGTAGTACAACTCTAGCTAT+TGG | 0.405934 | 8.1:-15480174 | MS.gene80558:CDS |
| GCATTAGCTCTGATTATTGA+TGG | 0.420012 | 8.1:-15480336 | MS.gene80558:CDS |
| AAAAGCACTTGTTACTAGAC+TGG | 0.497916 | 8.1:-15480211 | MS.gene80558:CDS |
| CAGTGGTGTCGAAGGAATGC+AGG | 0.518183 | 8.1:-15480097 | MS.gene80558:CDS |
| CAAGAAGCAGATATTGGAAT+CGG | 0.522135 | 8.1:-15480123 | MS.gene80558:CDS |
| TACTTCTTGTGCATGGACAT+TGG | 0.532635 | 8.1:-15480023 | MS.gene80558:CDS |
| CATTAGCTCTGATTATTGAT+GGG | 0.536630 | 8.1:-15480335 | MS.gene80558:CDS |
| AAGTGTTGTTCAACAAATAA+CGG | 0.550104 | 8.1:-15480400 | MS.gene80558:CDS |
| GAACGCTTACTTCTTGTGCA+TGG | 0.562953 | 8.1:-15480030 | MS.gene80558:CDS |
| GGAGATGGTGCAAATGATGT+TGG | 0.567615 | 8.1:-15480153 | MS.gene80558:CDS |
| TTACAGAAGGATCTCATCAA+TGG | 0.569495 | 8.1:-15479998 | None:intergenic |
| ATCGGTATCAGTGGTGTCGA+AGG | 0.574345 | 8.1:-15480105 | MS.gene80558:CDS |
| GTGTTTCTTGAACTTGCGAT+TGG | 0.578892 | 8.1:-15480273 | MS.gene80558:CDS |
| TCAACAAATAACGGAGGCAA+AGG | 0.582308 | 8.1:-15480391 | MS.gene80558:CDS |
| GGACAAGTCTGCTGCTGAAG+CGG | 0.592086 | 8.1:-15480568 | MS.gene80558:intron |
| ACAACTCTAGCTATTGGAGA+TGG | 0.610032 | 8.1:-15480168 | MS.gene80558:CDS |
| GAGATATCTAAATTGAGCAA+TGG | 0.619414 | 8.1:+15480053 | None:intergenic |
| ATGGACATTGGTGTTACAGA+AGG | 0.621470 | 8.1:-15480011 | MS.gene80558:CDS |
| ATAGCTAGAGTTGTACTACC+AGG | 0.635627 | 8.1:+15480177 | None:intergenic |
| AGACTGGTTAAGATGAGACC+TGG | 0.640721 | 8.1:-15480195 | MS.gene80558:CDS |
| GATATTGGAATCGGTATCAG+TGG | 0.644422 | 8.1:-15480114 | MS.gene80558:CDS |
| AAAATCAGATAACAACTCTG+AGG | 0.683827 | 8.1:-15480358 | MS.gene80558:CDS |
| TGTTGTTCAACAAATAACGG+AGG | 0.744763 | 8.1:-15480397 | MS.gene80558:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGAACTAAAATCAATTTAAT+TGG | + | chr8.1:15480123-15480142 | None:intergenic | 15.0% |
| !!! | TAGATTAGAATCTTAATTTT+TGG | - | chr8.1:15480068-15480087 | MS.gene80558:CDS | 15.0% |
| !! | AAGTGTTGTTCAACAAATAA+CGG | - | chr8.1:15480172-15480191 | MS.gene80558:CDS | 25.0% |
| AAAATCAGATAACAACTCTG+AGG | - | chr8.1:15480214-15480233 | MS.gene80558:CDS | 30.0% | |
| GAGATATCTAAATTGAGCAA+TGG | + | chr8.1:15480522-15480541 | None:intergenic | 30.0% | |
| TTCAATCTTTCTGATTCTTC+AGG | - | chr8.1:15480139-15480158 | MS.gene80558:CDS | 30.0% | |
| !! | CATTAGCTCTGATTATTGAT+GGG | - | chr8.1:15480237-15480256 | MS.gene80558:CDS | 30.0% |
| !! | GTAACAAGTGCTTTTTGTTT+TGG | + | chr8.1:15480356-15480375 | None:intergenic | 30.0% |
| CAAGAAGCAGATATTGGAAT+CGG | - | chr8.1:15480449-15480468 | MS.gene80558:intron | 35.0% | |
| TCTGATTCTTCAGGCAATTA+AGG | - | chr8.1:15480148-15480167 | MS.gene80558:CDS | 35.0% | |
| TGTTGTTCAACAAATAACGG+AGG | - | chr8.1:15480175-15480194 | MS.gene80558:CDS | 35.0% | |
| !! | AAAAGCACTTGTTACTAGAC+TGG | - | chr8.1:15480361-15480380 | MS.gene80558:CDS | 35.0% |
| !! | GCATTAGCTCTGATTATTGA+TGG | - | chr8.1:15480236-15480255 | MS.gene80558:CDS | 35.0% |
| ACAACTCTAGCTATTGGAGA+TGG | - | chr8.1:15480404-15480423 | MS.gene80558:CDS | 40.0% | |
| ATAGCTAGAGTTGTACTACC+AGG | + | chr8.1:15480398-15480417 | None:intergenic | 40.0% | |
| ATGCTCCAAGAAGCAGATAT+TGG | - | chr8.1:15480443-15480462 | MS.gene80558:intron | 40.0% | |
| CGATTCCAATATCTGCTTCT+TGG | + | chr8.1:15480451-15480470 | None:intergenic | 40.0% | |
| GATATTGGAATCGGTATCAG+TGG | - | chr8.1:15480458-15480477 | MS.gene80558:intron | 40.0% | |
| GGTAGTACAACTCTAGCTAT+TGG | - | chr8.1:15480398-15480417 | MS.gene80558:CDS | 40.0% | |
| GTGTTTCTTGAACTTGCGAT+TGG | - | chr8.1:15480299-15480318 | MS.gene80558:CDS | 40.0% | |
| TACTTCTTGTGCATGGACAT+TGG | - | chr8.1:15480549-15480568 | MS.gene80558:intron | 40.0% | |
| ! | TCAACAAATAACGGAGGCAA+AGG | - | chr8.1:15480181-15480200 | MS.gene80558:CDS | 40.0% |
| !! | ATGGACATTGGTGTTACAGA+AGG | - | chr8.1:15480561-15480580 | MS.gene80558:intron | 40.0% |
| GAACGCTTACTTCTTGTGCA+TGG | - | chr8.1:15480542-15480561 | MS.gene80558:intron | 45.0% | |
| GGAGATGGTGCAAATGATGT+TGG | - | chr8.1:15480419-15480438 | MS.gene80558:intron | 45.0% | |
| ! | AGACTGGTTAAGATGAGACC+TGG | - | chr8.1:15480377-15480396 | MS.gene80558:CDS | 45.0% |
| ! | ATCGGTATCAGTGGTGTCGA+AGG | - | chr8.1:15480467-15480486 | MS.gene80558:intron | 50.0% |
| GGACAAGTCTGCTGCTGAAG+CGG | - | chr8.1:15480004-15480023 | MS.gene80558:CDS | 55.0% | |
| ! | CAGTGGTGTCGAAGGAATGC+AGG | - | chr8.1:15480475-15480494 | MS.gene80558:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 15479999 | 15480595 | 15479999 | ID=MS.gene80558 |
| chr8.1 | mRNA | 15479999 | 15480595 | 15479999 | ID=MS.gene80558.t1;Parent=MS.gene80558 |
| chr8.1 | exon | 15480569 | 15480595 | 15480569 | ID=MS.gene80558.t1.exon1;Parent=MS.gene80558.t1 |
| chr8.1 | CDS | 15480569 | 15480595 | 15480569 | ID=cds.MS.gene80558.t1;Parent=MS.gene80558.t1 |
| chr8.1 | exon | 15479999 | 15480433 | 15479999 | ID=MS.gene80558.t1.exon2;Parent=MS.gene80558.t1 |
| chr8.1 | CDS | 15479999 | 15480433 | 15479999 | ID=cds.MS.gene80558.t1;Parent=MS.gene80558.t1 |
| Gene Sequence |
| Protein sequence |
