AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene80641


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80641.t1 MTR_5g081740 95.307 277 12 1 1 276 21 297 0.0 522
MS.gene80641.t1 MTR_5g081740 96.209 211 7 1 1 210 21 231 2.32e-148 414
MS.gene80641.t1 MTR_5g081740 96.209 211 7 1 1 210 21 231 8.58e-148 413
MS.gene80641.t1 MTR_3g070090 62.409 274 96 2 5 273 28 299 9.84e-124 355
MS.gene80641.t1 MTR_5g081740 96.622 148 4 1 1 147 21 168 2.23e-101 293
MS.gene80641.t1 MTR_7g092870 94.531 128 7 0 1 128 21 148 2.60e-78 234
MS.gene80641.t1 MTR_7g092875 91.753 97 8 0 164 260 1 97 3.99e-61 188
MS.gene80641.t1 MTR_8g046260 28.302 212 138 5 66 266 47 255 1.19e-19 86.3
MS.gene80641.t1 MTR_1g073610 25.943 212 146 5 64 266 40 249 7.86e-18 80.9
MS.gene80641.t1 MTR_6g090070 26.761 213 144 4 64 266 38 248 1.13e-16 77.8
MS.gene80641.t1 MTR_1g045120 23.600 250 164 8 31 266 14 250 2.89e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80641.t1 AT2G39920 39.706 272 141 5 1 268 23 275 2.23e-59 190
MS.gene80641.t1 AT2G39920 39.706 272 141 5 1 268 23 275 2.23e-59 190
MS.gene80641.t1 AT2G39920 39.706 272 141 5 1 268 23 275 2.23e-59 190
MS.gene80641.t1 AT2G39920 39.338 272 141 5 1 268 23 274 7.28e-59 189
MS.gene80641.t1 AT4G29270 33.178 214 129 6 64 266 42 252 2.21e-26 104
MS.gene80641.t1 AT4G29260 29.778 225 130 7 60 266 37 251 3.80e-18 82.0
MS.gene80641.t1 AT4G25150 24.715 263 167 7 28 266 1 256 2.09e-17 80.1
MS.gene80641.t1 AT5G51260 25.926 189 120 4 65 238 44 227 3.56e-15 73.9
MS.gene80641.t1 AT5G44020 24.017 229 157 7 52 266 43 268 3.34e-14 71.2
MS.gene80641.t1 AT1G04040 23.605 233 162 6 48 266 37 267 9.95e-13 67.0

Find 38 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATGCTAAGCTCAATAATT+TGG 0.240869 5.4:-64431280 MS.gene80641:CDS
AAGGAATGTGATACAGAAAA+AGG 0.244662 5.4:-64429857 MS.gene80641:CDS
GTTGATTGATGGTGACATTT+TGG 0.278987 5.4:+64430311 None:intergenic
AAATTACCTTAGTAACAAAT+TGG 0.311682 5.4:+64431065 None:intergenic
CTTGTTGATGCTGGATTTAG+AGG 0.329191 5.4:-64430289 MS.gene80641:CDS
ATTGCTGGATTTGCAGCTCT+TGG 0.339380 5.4:-64431429 MS.gene80641:CDS
GTAATCCTCAATCACAGATT+TGG 0.344974 5.4:+64431173 None:intergenic
TCTGCAACAATCTTCATTGC+TGG 0.362031 5.4:-64431444 MS.gene80641:CDS
ATTCTTCCAATTTGTTACTA+AGG 0.365366 5.4:-64431071 MS.gene80641:intron
AGTCACTTTGTTGGTTTCAA+TGG 0.374169 5.4:-64431397 MS.gene80641:CDS
GGCTCAAACATGTCACATAT+AGG 0.417441 5.4:+64429742 None:intergenic
ATCAATCAACTTGTTGATGC+TGG 0.420104 5.4:-64430298 MS.gene80641:CDS
AGCATCAACAAGTTGATTGA+TGG 0.423312 5.4:+64430300 None:intergenic
GCTCAAACATGTCACATATA+GGG 0.427523 5.4:+64429743 None:intergenic
GCCAGGTCTTTACAAATGTT+AGG 0.429891 5.4:+64431243 None:intergenic
TAGACCAACCACTAGCTTGA+AGG 0.432274 5.4:+64430365 None:intergenic
ACTGTTACAGATGCAGATAC+AGG 0.440440 5.4:-64429790 MS.gene80641:CDS
TCATGTCAAAATAGCAATGC+TGG 0.451480 5.4:-64431360 MS.gene80641:CDS
TTGATGCTGGATTTAGAGGC+TGG 0.460574 5.4:-64430285 MS.gene80641:CDS
TTCAACCAAATCTGTGATTG+AGG 0.476332 5.4:-64431178 MS.gene80641:CDS
AGTAACAAATTGGAAGAATG+AGG 0.486000 5.4:+64431075 None:intergenic
GTGATTGAGGATTACTTCAA+TGG 0.491347 5.4:-64431165 MS.gene80641:CDS
AACATGTCACATATAGGGTC+TGG 0.494617 5.4:+64429748 None:intergenic
GGTGTTAAACCATCAGAAGA+TGG 0.502777 5.4:-64431144 MS.gene80641:CDS
ATGCTGGAATTGTTGAGCTT+AGG 0.504865 5.4:-64431344 MS.gene80641:CDS
CTTTGATATATTGTAAAGCC+AGG 0.529642 5.4:+64431226 None:intergenic
TCCTAACATTTGTAAAGACC+TGG 0.529775 5.4:-64431244 MS.gene80641:CDS
TGGTCAATATGCAAGAGACT+TGG 0.581585 5.4:-64431202 MS.gene80641:CDS
TACAAGAACCTTCAAGCTAG+TGG 0.590030 5.4:-64430373 MS.gene80641:CDS
ACATCAAAACCATCTTCTGA+TGG 0.590480 5.4:+64431135 None:intergenic
GCTGGTCTTCATTGCTGATG+AGG 0.591699 5.4:-64430267 MS.gene80641:intron
AAGTATCATAAGCAGTCATG+TGG 0.611564 5.4:-64429821 MS.gene80641:CDS
TGAGCCTCAGAGGAGAGCAT+AGG 0.617171 5.4:-64429725 None:intergenic
CTGGCTTTACAATATATCAA+AGG 0.617801 5.4:-64431225 MS.gene80641:CDS
AGCACCTATGCTCTCCTCTG+AGG 0.619362 5.4:+64429721 None:intergenic
AGAACCTTCAAGCTAGTGGT+TGG 0.622868 5.4:-64430369 MS.gene80641:CDS
GCTTTACAATATATCAAAGG+TGG 0.628623 5.4:-64431222 MS.gene80641:CDS
GTGACATGTTTGAGCCTCAG+AGG 0.633336 5.4:-64429735 MS.gene80641:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTATTAGTTAAGTTTT+TGG - chr5.4:64430198-64430217 MS.gene80641:intron 10.0%
!!! TAATTTTATCATTTAAGATT+TGG - chr5.4:64430170-64430189 MS.gene80641:intron 10.0%
!!! TTTTTATTAGTTAAGTTTTT+GGG - chr5.4:64430199-64430218 MS.gene80641:intron 10.0%
!!! TTTAACATTATCTAGAATTA+AGG + chr5.4:64430545-64430564 None:intergenic 15.0%
!! AAATTACCTTAGTAACAAAT+TGG + chr5.4:64430156-64430175 None:intergenic 20.0%
!! TATCTATAAAACAACATTTG+TGG + chr5.4:64431021-64431040 None:intergenic 20.0%
!!! ATGAATATTTTTCTAGACAA+AGG - chr5.4:64431342-64431361 MS.gene80641:CDS 20.0%
!!! TGTTGTTTTATAGATATCTA+AGG - chr5.4:64431025-64431044 MS.gene80641:intron 20.0%
! ATGAAATTACATCTTCAAAG+TGG + chr5.4:64430442-64430461 None:intergenic 25.0%
! ATTAGTAAACTAATGCAAGT+TGG + chr5.4:64430628-64430647 None:intergenic 25.0%
! TCTGCAAAAATAGAACATAT+TGG + chr5.4:64431294-64431313 None:intergenic 25.0%
! TTTACTAATATTGCAAAACC+AGG + chr5.4:64430377-64430396 None:intergenic 25.0%
!! AACCATTATCTAGGATATAT+GGG + chr5.4:64430989-64431008 None:intergenic 25.0%
!! TCTAGAATTAAGGTACATAA+TGG + chr5.4:64430535-64430554 None:intergenic 25.0%
!!! ACATAGATAGTCTTTTTCAA+TGG - chr5.4:64430117-64430136 MS.gene80641:intron 25.0%
!!! ACTTTTATTAGTCACTTTGT+TGG - chr5.4:64429812-64429831 MS.gene80641:CDS 25.0%
!!! ATTCTTCCAATTTGTTACTA+AGG - chr5.4:64430147-64430166 MS.gene80641:intron 25.0%
!!! TTAGTTAAGTTTTTGGGATT+TGG - chr5.4:64430205-64430224 MS.gene80641:intron 25.0%
AAAGTACATGTTTGGATTGA+CGG - chr5.4:64431125-64431144 MS.gene80641:CDS 30.0%
AACATGTCACATATACTTTG+TGG + chr5.4:64431206-64431225 None:intergenic 30.0%
AAGGAATGTGATACAGAAAA+AGG - chr5.4:64431361-64431380 MS.gene80641:CDS 30.0%
ACAGATAAATGCTAAGATCT+AGG - chr5.4:64430566-64430585 MS.gene80641:intron 30.0%
AGGAATGTGATACAGAAAAA+GGG - chr5.4:64431362-64431381 MS.gene80641:CDS 30.0%
AGTAACAAATTGGAAGAATG+AGG + chr5.4:64430146-64430165 None:intergenic 30.0%
ATTCTTGACTGCAAGTAATT+CGG - chr5.4:64430349-64430368 MS.gene80641:CDS 30.0%
CTTTGATATATTGTAAAGCC+AGG + chr5.4:64429995-64430014 None:intergenic 30.0%
GCTTTACAATATATCAAAGG+TGG - chr5.4:64429996-64430015 MS.gene80641:intron 30.0%
GGATTTGGATTTCATTTAGT+CGG - chr5.4:64430220-64430239 MS.gene80641:intron 30.0%
TCGATTACAAAGTACATGTT+TGG - chr5.4:64431117-64431136 MS.gene80641:CDS 30.0%
TCTCATACTTTGTGTATGTT+TGG + chr5.4:64431254-64431273 None:intergenic 30.0%
! AACTTGACAAGGTTTTCTTT+TGG - chr5.4:64430742-64430761 MS.gene80641:intron 30.0%
! ACATATTGGTGCTTAAAATC+TGG + chr5.4:64431280-64431299 None:intergenic 30.0%
! CAACCATTATCTAGGATATA+TGG + chr5.4:64430990-64431009 None:intergenic 30.0%
! CATATATCCTAGATAATGGT+TGG - chr5.4:64430988-64431007 MS.gene80641:intron 30.0%
! TTTTTCTCCAACCATTATCT+AGG + chr5.4:64430998-64431017 None:intergenic 30.0%
!! ATTTTGTTATCAAGAGAGTC+AGG - chr5.4:64430878-64430897 MS.gene80641:intron 30.0%
!! CTGGCTTTACAATATATCAA+AGG - chr5.4:64429993-64430012 MS.gene80641:intron 30.0%
!! TTAAGGTACATAATGGATAG+AGG + chr5.4:64430528-64430547 None:intergenic 30.0%
AAGTATCATAAGCAGTCATG+TGG - chr5.4:64431397-64431416 MS.gene80641:CDS 35.0%
ACATCAAAACCATCTTCTGA+TGG + chr5.4:64430086-64430105 None:intergenic 35.0%
CAATGAGTTTGGCAAAATCA+CGG + chr5.4:64431178-64431197 None:intergenic 35.0%
CACCCATATATCCTAGATAA+TGG - chr5.4:64430984-64431003 MS.gene80641:intron 35.0%
GCATGCTAAGCTCAATAATT+TGG - chr5.4:64429938-64429957 MS.gene80641:intron 35.0%
GCTCAAACATGTCACATATA+GGG + chr5.4:64431478-64431497 None:intergenic 35.0%
GTAATCCTCAATCACAGATT+TGG + chr5.4:64430048-64430067 None:intergenic 35.0%
GTGATTGAGGATTACTTCAA+TGG - chr5.4:64430053-64430072 MS.gene80641:intron 35.0%
TATGCAGATTAGCATCAATC+TGG + chr5.4:64430709-64430728 None:intergenic 35.0%
TCATGTCAAAATAGCAATGC+TGG - chr5.4:64429858-64429877 MS.gene80641:CDS 35.0%
TCCTAACATTTGTAAAGACC+TGG - chr5.4:64429974-64429993 MS.gene80641:intron 35.0%
TGATACAGAAAAAGGGTTTC+AGG - chr5.4:64431369-64431388 MS.gene80641:CDS 35.0%
TTCAACCAAATCTGTGATTG+AGG - chr5.4:64430040-64430059 MS.gene80641:intron 35.0%
TTGTGGATGAACAATGAGTT+TGG + chr5.4:64431189-64431208 None:intergenic 35.0%
TTGTTAGCAGTAACTTGACA+AGG - chr5.4:64430731-64430750 MS.gene80641:intron 35.0%
! CATTATCTAGGATATATGGG+TGG + chr5.4:64430986-64431005 None:intergenic 35.0%
!! AACGGTCTGAATTTTGAGTA+TGG + chr5.4:64430308-64430327 None:intergenic 35.0%
!! ACAAGGTTTTCTTTTGGTGT+TGG - chr5.4:64430748-64430767 MS.gene80641:intron 35.0%
!! AGCATCAACAAGTTGATTGA+TGG + chr5.4:64430921-64430940 None:intergenic 35.0%
!! AGTCACTTTGTTGGTTTCAA+TGG - chr5.4:64429821-64429840 MS.gene80641:CDS 35.0%
!! ATCAATCAACTTGTTGATGC+TGG - chr5.4:64430920-64430939 MS.gene80641:intron 35.0%
!!! ATCAGAAGATGGTTTTGATG+TGG - chr5.4:64430085-64430104 MS.gene80641:intron 35.0%
!!! GTTGATTGATGGTGACATTT+TGG + chr5.4:64430910-64430929 None:intergenic 35.0%
AACATGTCACATATAGGGTC+TGG + chr5.4:64431473-64431492 None:intergenic 40.0%
ACTGTTACAGATGCAGATAC+AGG - chr5.4:64431428-64431447 MS.gene80641:CDS 40.0%
ATGCTGGAATTGTTGAGCTT+AGG - chr5.4:64429874-64429893 MS.gene80641:CDS 40.0%
CACGGTTAGAAAAAGACGAA+CGG + chr5.4:64430326-64430345 None:intergenic 40.0%
CGGAAAACTTAAAGTCGAGA+CGG - chr5.4:64430240-64430259 MS.gene80641:intron 40.0%
CTTGCAGTCAAGAATCTACA+CGG + chr5.4:64430344-64430363 None:intergenic 40.0%
CTTGTTGATGCTGGATTTAG+AGG - chr5.4:64430929-64430948 MS.gene80641:intron 40.0%
GCCAGGTCTTTACAAATGTT+AGG + chr5.4:64429978-64429997 None:intergenic 40.0%
GGCTCAAACATGTCACATAT+AGG + chr5.4:64431479-64431498 None:intergenic 40.0%
GGTGTTAAACCATCAGAAGA+TGG - chr5.4:64430074-64430093 MS.gene80641:intron 40.0%
TACAAGAACCTTCAAGCTAG+TGG - chr5.4:64430845-64430864 MS.gene80641:intron 40.0%
TCTGCAACAATCTTCATTGC+TGG - chr5.4:64429774-64429793 MS.gene80641:CDS 40.0%
TGGTCAATATGCAAGAGACT+TGG - chr5.4:64430016-64430035 MS.gene80641:intron 40.0%
!! AGCAGTTTTGTGCTTGAATC+AGG - chr5.4:64429732-64429751 MS.gene80641:CDS 40.0%
ACTGCAAGTAATTCGGATCC+TGG - chr5.4:64430356-64430375 MS.gene80641:CDS 45.0%
AGAACCTTCAAGCTAGTGGT+TGG - chr5.4:64430849-64430868 MS.gene80641:intron 45.0%
ATTGCTGGATTTGCAGCTCT+TGG - chr5.4:64429789-64429808 MS.gene80641:CDS 45.0%
TGAGTTTGGCAAAATCACGG+TGG + chr5.4:64431175-64431194 None:intergenic 45.0%
TTGATGCTGGATTTAGAGGC+TGG - chr5.4:64430933-64430952 MS.gene80641:intron 45.0%
! ATGCTAAGATCTAGGACAGC+TGG - chr5.4:64430574-64430593 MS.gene80641:intron 45.0%
! TAGACCAACCACTAGCTTGA+AGG + chr5.4:64430856-64430875 None:intergenic 45.0%
GTGACATGTTTGAGCCTCAG+AGG - chr5.4:64431483-64431502 MS.gene80641:CDS 50.0%
!! GCTGGTCTTCATTGCTGATG+AGG - chr5.4:64430951-64430970 MS.gene80641:intron 50.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 64429726 64431514 64429726 ID=MS.gene80641
chr5.4 mRNA 64429726 64431514 64429726 ID=MS.gene80641.t1;Parent=MS.gene80641
chr5.4 exon 64431072 64431514 64431072 ID=MS.gene80641.t1.exon1;Parent=MS.gene80641.t1
chr5.4 CDS 64431072 64431514 64431072 ID=cds.MS.gene80641.t1;Parent=MS.gene80641.t1
chr5.4 exon 64430268 64430453 64430268 ID=MS.gene80641.t1.exon2;Parent=MS.gene80641.t1
chr5.4 CDS 64430268 64430453 64430268 ID=cds.MS.gene80641.t1;Parent=MS.gene80641.t1
chr5.4 exon 64429726 64429927 64429726 ID=MS.gene80641.t1.exon3;Parent=MS.gene80641.t1
chr5.4 CDS 64429726 64429927 64429726 ID=cds.MS.gene80641.t1;Parent=MS.gene80641.t1
Gene Sequence

>MS.gene80641

ATGGGGAGCAGTTTTGTGCTTGAATCAGGTTTCTACATCACATCATTTTCTGCAACAATCTTCATTGCTGGATTTGCAGCTCTTGGACTTTTATTAGTCACTTTGTTGGTTTCAATGGCGATGATGCTACAATCATGTCAAAATAGCAATGCTGGAATTGTTGAGCTTAGGAATATAAATGATGACTATAGTTATTGCAAAATTCATTCTCTGCATGCTAAGCTCAATAATTTGGAAGAATATAATGTTCCTAACATTTGTAAAGACCTGGCTTTACAATATATCAAAGGTGGTCAATATGCAAGAGACTTGGATTCAACCAAATCTGTGATTGAGGATTACTTCAATGGTGTTAAACCATCAGAAGATGGTTTTGATGTGGTGTTGATAGACATAGATAGTCTTTTTCAATGGAATCCTCATTCTTCCAATTTGTTACTAAGGTAATTTTATCATTTAAGATTTGGATTTTATTTTTATTAGTTAAGTTTTTGGGATTTGGATTTCATTTAGTCGGAAAACTTAAAGTCGAGACGGCTCATGATGTATAGTTTTGAATTTGTGTTTGATATTTATTTTCCATACTCAAAATTCAGACCGTTCGTCTTTTTCTAACCGTGTAGATTCTTGACTGCAAGTAATTCGGATCCTGGTTTTGCAATATTAGTAAAGCAAATTCTACTTTCTTGTCTTATTTATGATAGTACATTTCACCACTTTGAAGATGTAATTTCATATAGAAAAATTTGAAGACTTGTAATCTGAATCTTCATGAACTTTGTTTATAGTATTAGCAGATCCTCTATCCATTATGTACCTTAATTCTAGATAATGTTAAATACAGATAAATGCTAAGATCTAGGACAGCTGGAATGTTTGTGTCTTAATAATTTTTTACTCCAACTTGCATTAGTTTACTAATTTACATTCATGCTATAACAGCTCAAATGTAATTATTGTACATTTTTTATGTAGCTAATCCAGATTGATGCTAATCTGCATATTTTGTTAGCAGTAACTTGACAAGGTTTTCTTTTGGTGTTGGATTTTCATTATAACAGCATTAGCAATTGTATCATCGACGCAAAGAATTTAAAACGCGTGCTTATGTTAAGAATATACAAGAACCTTCAAGCTAGTGGTTGGTCTATAATTTTGTTATCAAGAGAGTCAGGAACACACCAAAATGTCACCATCAATCAACTTGTTGATGCTGGATTTAGAGGCTGGTCTTCATTGCTGATGAGGTATTATGTTCCACCCATATATCCTAGATAATGGTTGGAGAAAAACCACAAATGTTGTTTTATAGATATCTAAGGTGTCTTTGCTTTATTGTTTATTTAATTATTAATTACTATGCATTTAATTATTTTTAGTTAAGATTATATTCGATTACAAAGTACATGTTTGGATTGACGGTGTGATTGTCAAAATCACATTGAACCACCGTGATTTTGCCAAACTCATTGTTCATCCACAAAGTATATGTGACATGTTTACTAGTATATTGCTACACTTTGTACCAAACATACACAAAGTATGAGAGAACCAGATTTTAAGCACCAATATGTTCTATTTTTGCAGAGCAGAAGATGAAGATTCTATCAAAGCAAATGAATATTTTTCTAGACAAAGGAATGTGATACAGAAAAAGGGTTTCAGGATAAAAAGTATCATAAGCAGTCATGTGGATATTTTGACTGTTACAGATGCAGATACAGGAATGCGAAAGTTTTTGCTACCAGACCCTATATGTGACATGTTTGAGCCTCAGAGGAGAGCATAG

Protein sequence

>MS.gene80641.t1

MGSSFVLESGFYITSFSATIFIAGFAALGLLLVTLLVSMAMMLQSCQNSNAGIVELRNINDDYSYCKIHSLHAKLNNLEEYNVPNICKDLALQYIKGGQYARDLDSTKSVIEDYFNGVKPSEDGFDVVLIDIDSLFQWNPHSSNLLLSISNCIIDAKNLKRVLMLRIYKNLQASGWSIILLSRESGTHQNVTINQLVDAGFRGWSSLLMRAEDEDSIKANEYFSRQRNVIQKKGFRIKSIISSHVDILTVTDADTGMRKFLLPDPICDMFEPQRRA