AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82311


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82311.t1 MTR_4g065200 69.307 101 27 1 39 139 359 455 9.88e-40 142
MS.gene82311.t1 MTR_4g065150 66.990 103 30 1 37 139 359 457 1.14e-38 139
MS.gene82311.t1 MTR_4g065220 64.356 101 32 1 39 139 364 460 3.59e-37 134
MS.gene82311.t1 MTR_1g030480 86.364 66 9 0 1 66 310 375 1.67e-27 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82311.t1 AT5G12960 63.636 99 31 2 40 138 369 462 5.31e-34 125
MS.gene82311.t1 AT5G12950 60.952 105 36 2 34 138 359 458 6.69e-34 125
MS.gene82311.t1 AT1G44446 76.119 67 16 0 1 67 318 384 2.86e-24 97.4
MS.gene82311.t1 AT1G44446 75.758 66 16 0 1 66 318 383 3.19e-23 94.7
MS.gene82311.t1 AT1G44446 75.758 66 16 0 1 66 216 281 3.22e-23 94.4
MS.gene82311.t1 AT1G44446 75.758 66 16 0 1 66 318 383 3.43e-23 94.7

Find 42 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTACCTCCTCCTGGATTTA+TGG 0.157910 3.4:-21714525 MS.gene82311:CDS
AGAAGGTAGTGTGGCTGTTT+GGG 0.259935 3.4:+21714550 None:intergenic
AATGTTGAAGTATGAGTAAA+AGG 0.289215 3.4:+21714270 None:intergenic
ATGATTTATATGCTTCCTTT+AGG 0.300957 3.4:-21713056 MS.gene82311:intron
AAGAAGGTAGTGTGGCTGTT+TGG 0.320416 3.4:+21714549 None:intergenic
AAGATGTCAGCTATTCTCTT+TGG 0.324038 3.4:+21713489 None:intergenic
CTTATGCCTCATTTATAGAT+TGG 0.324997 3.4:-21712903 MS.gene82311:CDS
TTTCGCGGCACCTGTTTAGA+TGG 0.351657 3.4:-21713166 MS.gene82311:CDS
AAGAAGGAATGATCTGGATT+TGG 0.390925 3.4:-21714586 None:intergenic
ACAGATTGTCATGAAACTTC+CGG 0.392781 3.4:-21714340 MS.gene82311:intron
TTGAGAAAGAAGGAATGATC+TGG 0.433991 3.4:-21714592 None:intergenic
AAAAGTAAGCCATGTTCAAC+CGG 0.444457 3.4:+21714321 None:intergenic
CCTTCTTTACTACCTCCTCC+TGG 0.448150 3.4:-21714533 MS.gene82311:CDS
CACAACTTACAATATGTTGA+AGG 0.454540 3.4:-21713439 MS.gene82311:intron
CTAAGGGATGGAGTGTTCCC+AGG 0.462886 3.4:-21714245 MS.gene82311:intron
ATTTATAGATTGGAATATTG+AGG 0.463853 3.4:-21712893 MS.gene82311:CDS
ATACAAAGAGGAACAGATCC+TGG 0.477044 3.4:-21713083 MS.gene82311:CDS
GCTGTTTGGGGATCACTGCC+AGG 0.478702 3.4:+21714563 None:intergenic
TCATACTTCAACATTTGCTA+AGG 0.484176 3.4:-21714262 MS.gene82311:CDS
CAACATATTGTAAGTTGTGA+AGG 0.493601 3.4:+21713443 None:intergenic
CCAGGAGGAGGTAGTAAAGA+AGG 0.503777 3.4:+21714533 None:intergenic
GATAATGATTTCAGGTTTCG+CGG 0.508807 3.4:-21713181 MS.gene82311:intron
ACTGAGTACACCATTCGTTA+AGG 0.509378 3.4:+21713106 None:intergenic
CTTCAACATTTGCTAAGGGA+TGG 0.527830 3.4:-21714257 MS.gene82311:CDS
TACAAAGAGGAACAGATCCT+GGG 0.532747 3.4:-21713082 MS.gene82311:CDS
CATACTTCAACATTTGCTAA+GGG 0.539291 3.4:-21714261 MS.gene82311:CDS
AGGTAGTAAAGAAGGTAGTG+TGG 0.544537 3.4:+21714541 None:intergenic
TGGATTTATGGTCCTTGCCG+AGG 0.545672 3.4:-21714513 MS.gene82311:intron
AATATTCCAATCTATAAATG+AGG 0.548260 3.4:+21712897 None:intergenic
GGAATGATCTGGATTTGGCC+TGG 0.548326 3.4:-21714581 MS.gene82311:CDS
ATGAAACTTCCGGTTGAACA+TGG 0.550052 3.4:-21714330 MS.gene82311:CDS
TATGAACGTGCCTTAACGAA+TGG 0.556600 3.4:-21713116 MS.gene82311:CDS
ATGTTGAAGTATGAGTAAAA+GGG 0.570683 3.4:+21714271 None:intergenic
GGACGTGCGCAAGATCCAAA+AGG 0.571017 3.4:+21714292 None:intergenic
AGCATATAAATCATCACCCC+AGG 0.579509 3.4:+21713065 None:intergenic
TCGGCAAGGACCATAAATCC+AGG 0.583930 3.4:+21714515 None:intergenic
GAAGGTAGTGTGGCTGTTTG+GGG 0.598304 3.4:+21714551 None:intergenic
AGGACCATAAATCCAGGAGG+AGG 0.624161 3.4:+21714521 None:intergenic
ACTTATTATATACCTCGGCA+AGG 0.666840 3.4:+21714501 None:intergenic
GGTGTACTCAGTATACAAAG+AGG 0.682670 3.4:-21713095 MS.gene82311:CDS
ACAAAGAGGAACAGATCCTG+GGG 0.705144 3.4:-21713081 MS.gene82311:CDS
GCAAGGACCATAAATCCAGG+AGG 0.751688 3.4:+21714518 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAACTTTTTTTTTAAGAAG+AGG - chr3.4:21713418-21713437 MS.gene82311:intron 15.0%
!! AAAAAAGAAGATAAAAGTCA+TGG - chr3.4:21713077-21713096 MS.gene82311:CDS 20.0%
!! AATATTCCAATCTATAAATG+AGG + chr3.4:21714572-21714591 None:intergenic 20.0%
!! ACTATACTTATTATATACCT+CGG + chr3.4:21712973-21712992 None:intergenic 20.0%
!! ATTTATAGATTGGAATATTG+AGG - chr3.4:21714573-21714592 MS.gene82311:CDS 20.0%
!! CTGCTAAATTTATTATCATA+TGG - chr3.4:21714115-21714134 MS.gene82311:intron 20.0%
!! TAATCCAATAAAACAACTTT+AGG + chr3.4:21714524-21714543 None:intergenic 20.0%
!! TATTAACTCAGAAAAAACAT+AGG + chr3.4:21713391-21713410 None:intergenic 20.0%
!! TGAAAAAAAAGGTAGTTTAT+TGG - chr3.4:21714152-21714171 MS.gene82311:intron 20.0%
!! TTTATTTGATCATATTTACC+TGG - chr3.4:21713476-21713495 MS.gene82311:CDS 20.0%
!!! CATGGAATTTATTTTCTTAA+AGG - chr3.4:21713856-21713875 MS.gene82311:intron 20.0%
!!! CTTTAAGAAAATAAATTCCA+TGG + chr3.4:21713858-21713877 None:intergenic 20.0%
!!! TTTGTTCCTATATTTTAAGA+TGG - chr3.4:21713896-21713915 MS.gene82311:intron 20.0%
!!! TTTTTCTTTTGACAACTTTA+GGG - chr3.4:21713350-21713369 MS.gene82311:intron 20.0%
! AACTATTTCAGAAAGATGTT+TGG + chr3.4:21713928-21713947 None:intergenic 25.0%
! AACTGACCATCTTAAAATAT+AGG + chr3.4:21713905-21713924 None:intergenic 25.0%
! AAGAGTTTGTCATAACAAAT+TGG + chr3.4:21713323-21713342 None:intergenic 25.0%
! AATGTTGAAGTATGAGTAAA+AGG + chr3.4:21713199-21713218 None:intergenic 25.0%
! ATGATTTATATGCTTCCTTT+AGG - chr3.4:21714410-21714429 MS.gene82311:intron 25.0%
! ATGTTGAAGTATGAGTAAAA+GGG + chr3.4:21713198-21713217 None:intergenic 25.0%
! ATTATCATATGGTAGTTCAT+TGG - chr3.4:21714126-21714145 MS.gene82311:intron 25.0%
! ATTGAATCATATCACTTCAA+AGG + chr3.4:21713263-21713282 None:intergenic 25.0%
! CTAAAGTTGTCAAAAGAAAA+AGG + chr3.4:21713351-21713370 None:intergenic 25.0%
! TAAGTATAGTATGTCCTAAT+AGG - chr3.4:21713005-21713024 MS.gene82311:intron 25.0%
! TAGTTCTTCATGTAATCTTA+GGG - chr3.4:21714075-21714094 MS.gene82311:intron 25.0%
! TCAAATAAACAAACTCAAAC+AGG + chr3.4:21713465-21713484 None:intergenic 25.0%
! TTCATTGGACTTGAAAAAAA+AGG - chr3.4:21714141-21714160 MS.gene82311:intron 25.0%
! TTGAATCATATCACTTCAAA+GGG + chr3.4:21713262-21713281 None:intergenic 25.0%
!! CTTTTTCTTTTGACAACTTT+AGG - chr3.4:21713349-21713368 MS.gene82311:intron 25.0%
!! TTAGTTCTTCATGTAATCTT+AGG - chr3.4:21714074-21714093 MS.gene82311:intron 25.0%
!!! CTTACTTTTTTACAACCTTT+TGG - chr3.4:21713159-21713178 MS.gene82311:CDS 25.0%
!!! CTTTACTTTACTTTTTCTGT+AGG - chr3.4:21713948-21713967 MS.gene82311:intron 25.0%
!!! TGTTTACATAACTGCTATTT+TGG + chr3.4:21713563-21713582 None:intergenic 25.0%
AGGATGTATCATCATAACTT+AGG - chr3.4:21713438-21713457 MS.gene82311:intron 30.0%
ATATGCTTCCTTTAGGTATT+GGG - chr3.4:21714417-21714436 MS.gene82311:intron 30.0%
CAACATATTGTAAGTTGTGA+AGG + chr3.4:21714026-21714045 None:intergenic 30.0%
CACAACTTACAATATGTTGA+AGG - chr3.4:21714027-21714046 MS.gene82311:intron 30.0%
CATAGCTCATAATACCTATT+AGG + chr3.4:21713022-21713041 None:intergenic 30.0%
CTTATGCCTCATTTATAGAT+TGG - chr3.4:21714563-21714582 MS.gene82311:CDS 30.0%
GGATGTATCATCATAACTTA+GGG - chr3.4:21713439-21713458 MS.gene82311:intron 30.0%
TATATGCTTCCTTTAGGTAT+TGG - chr3.4:21714416-21714435 MS.gene82311:intron 30.0%
TGTTGCAAGATAATGATTTC+AGG - chr3.4:21714277-21714296 MS.gene82311:CDS 30.0%
TTAATGTATCACACTGACAT+AGG + chr3.4:21714212-21714231 None:intergenic 30.0%
! CATACTTCAACATTTGCTAA+GGG - chr3.4:21713205-21713224 MS.gene82311:intron 30.0%
! CTGTTTTGTGAAAAAATACC+AGG + chr3.4:21713110-21713129 None:intergenic 30.0%
! TCATACTTCAACATTTGCTA+AGG - chr3.4:21713204-21713223 MS.gene82311:intron 30.0%
! TTACTCTCAATTGATTCAGT+AGG - chr3.4:21713769-21713788 MS.gene82311:intron 30.0%
!! TATGCTTTGAAAGTTATCCA+TGG - chr3.4:21713838-21713857 MS.gene82311:intron 30.0%
!! TCTGCCTAAAGTTGTTTTAT+TGG - chr3.4:21714517-21714536 MS.gene82311:CDS 30.0%
!!! ACTTTACTTTTTCTGTAGGA+AGG - chr3.4:21713952-21713971 MS.gene82311:intron 30.0%
AAAAGTAAGCCATGTTCAAC+CGG + chr3.4:21713148-21713167 None:intergenic 35.0%
AAAAGTCATGGTTTCTTACC+TGG - chr3.4:21713089-21713108 MS.gene82311:CDS 35.0%
AAGATGTCAGCTATTCTCTT+TGG + chr3.4:21713980-21713999 None:intergenic 35.0%
AAGCTAAAACTGGACATAGT+TGG - chr3.4:21714447-21714466 MS.gene82311:intron 35.0%
ACAGATTGTCATGAAACTTC+CGG - chr3.4:21713126-21713145 MS.gene82311:CDS 35.0%
ACTTATTATATACCTCGGCA+AGG + chr3.4:21712968-21712987 None:intergenic 35.0%
AGCTAAAACTGGACATAGTT+GGG - chr3.4:21714448-21714467 MS.gene82311:intron 35.0%
ATCCAATAATGATTCTGCTG+TGG - chr3.4:21714474-21714493 MS.gene82311:intron 35.0%
ATCCACAGCAGAATCATTAT+TGG + chr3.4:21714479-21714498 None:intergenic 35.0%
ATTTCGATATGTGCGTGAAA+TGG - chr3.4:21713689-21713708 MS.gene82311:intron 35.0%
CGCACATATCGAAATATCTA+AGG + chr3.4:21713684-21713703 None:intergenic 35.0%
! CTTCTTTTGTCCATCTAAAC+AGG + chr3.4:21714313-21714332 None:intergenic 35.0%
! GCACTGTTTGGAAAAGTATA+TGG - chr3.4:21713513-21713532 MS.gene82311:intron 35.0%
! TATGCTTCCTTTAGGTATTG+GGG - chr3.4:21714418-21714437 MS.gene82311:intron 35.0%
!! GATAATGATTTCAGGTTTCG+CGG - chr3.4:21714285-21714304 MS.gene82311:CDS 35.0%
!!! ACTATGTCCAGTTTTAGCTT+TGG + chr3.4:21714447-21714466 None:intergenic 35.0%
ACTGAGTACACCATTCGTTA+AGG + chr3.4:21714363-21714382 None:intergenic 40.0%
AGCATATAAATCATCACCCC+AGG + chr3.4:21714404-21714423 None:intergenic 40.0%
AGGTAGTAAAGAAGGTAGTG+TGG + chr3.4:21712928-21712947 None:intergenic 40.0%
ATACAAAGAGGAACAGATCC+TGG - chr3.4:21714383-21714402 MS.gene82311:intron 40.0%
ATGAAACTTCCGGTTGAACA+TGG - chr3.4:21713136-21713155 MS.gene82311:CDS 40.0%
GCTAAAACTGGACATAGTTG+GGG - chr3.4:21714449-21714468 MS.gene82311:intron 40.0%
GGCATAAACGATACAAACCT+GGG + chr3.4:21713241-21713260 None:intergenic 40.0%
GGTGTACTCAGTATACAAAG+AGG - chr3.4:21714371-21714390 MS.gene82311:intron 40.0%
TACAAAGAGGAACAGATCCT+GGG - chr3.4:21714384-21714403 MS.gene82311:intron 40.0%
TATGAACGTGCCTTAACGAA+TGG - chr3.4:21714350-21714369 MS.gene82311:intron 40.0%
TTGGAAACCCCAATACCTAA+AGG + chr3.4:21714428-21714447 None:intergenic 40.0%
! CTTCAACATTTGCTAAGGGA+TGG - chr3.4:21713209-21713228 MS.gene82311:intron 40.0%
AAGAAGGTAGTGTGGCTGTT+TGG + chr3.4:21712920-21712939 None:intergenic 45.0%
ACAAAGAGGAACAGATCCTG+GGG - chr3.4:21714385-21714404 MS.gene82311:intron 45.0%
ACTACCTCCTCCTGGATTTA+TGG - chr3.4:21712941-21712960 MS.gene82311:intron 45.0%
AGAAGGTAGTGTGGCTGTTT+GGG + chr3.4:21712919-21712938 None:intergenic 45.0%
GGGCATAAACGATACAAACC+TGG + chr3.4:21713242-21713261 None:intergenic 45.0%
GGGGTTTCCAAAGCTAAAAC+TGG - chr3.4:21714437-21714456 MS.gene82311:intron 45.0%
TAAGCACTTGTGGCACTGTT+TGG - chr3.4:21713501-21713520 MS.gene82311:intron 45.0%
TTACCTGGCATAAGCACTTG+TGG - chr3.4:21713491-21713510 MS.gene82311:CDS 45.0%
TTGATTCAGTAGGCGAGATC+TGG - chr3.4:21713779-21713798 MS.gene82311:intron 45.0%
!!! CTGCTATTTTGGAGAGCTCA+TGG + chr3.4:21713552-21713571 None:intergenic 45.0%
AGGACCATAAATCCAGGAGG+AGG + chr3.4:21712948-21712967 None:intergenic 50.0%
CCAGGAGGAGGTAGTAAAGA+AGG + chr3.4:21712936-21712955 None:intergenic 50.0%
CCTTCTTTACTACCTCCTCC+TGG - chr3.4:21712933-21712952 MS.gene82311:intron 50.0%
GAAGGTAGTGTGGCTGTTTG+GGG + chr3.4:21712918-21712937 None:intergenic 50.0%
GCAAGGACCATAAATCCAGG+AGG + chr3.4:21712951-21712970 None:intergenic 50.0%
TCGGCAAGGACCATAAATCC+AGG + chr3.4:21712954-21712973 None:intergenic 50.0%
TGGATTTATGGTCCTTGCCG+AGG - chr3.4:21712953-21712972 MS.gene82311:intron 50.0%
TTTCGCGGCACCTGTTTAGA+TGG - chr3.4:21714300-21714319 MS.gene82311:CDS 50.0%
GGACGTGCGCAAGATCCAAA+AGG + chr3.4:21713177-21713196 None:intergenic 55.0%
GTGCCACAAGTGCTTATGCC+AGG + chr3.4:21713497-21713516 None:intergenic 55.0%
! CTAAGGGATGGAGTGTTCCC+AGG - chr3.4:21713221-21713240 MS.gene82311:intron 55.0%
GCTGTTTGGGGATCACTGCC+AGG + chr3.4:21712906-21712925 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 21712888 21714600 21712888 ID=MS.gene82311
chr3.4 mRNA 21712888 21714600 21712888 ID=MS.gene82311.t1;Parent=MS.gene82311
chr3.4 exon 21714514 21714600 21714514 ID=MS.gene82311.t1.exon1;Parent=MS.gene82311.t1
chr3.4 CDS 21714514 21714600 21714514 ID=cds.MS.gene82311.t1;Parent=MS.gene82311.t1
chr3.4 exon 21714246 21714358 21714246 ID=MS.gene82311.t1.exon2;Parent=MS.gene82311.t1
chr3.4 CDS 21714246 21714358 21714246 ID=cds.MS.gene82311.t1;Parent=MS.gene82311.t1
chr3.4 exon 21713440 21713518 21713440 ID=MS.gene82311.t1.exon3;Parent=MS.gene82311.t1
chr3.4 CDS 21713440 21713518 21713440 ID=cds.MS.gene82311.t1;Parent=MS.gene82311.t1
chr3.4 exon 21713057 21713189 21713057 ID=MS.gene82311.t1.exon4;Parent=MS.gene82311.t1
chr3.4 CDS 21713057 21713189 21713057 ID=cds.MS.gene82311.t1;Parent=MS.gene82311.t1
chr3.4 exon 21712888 21712937 21712888 ID=MS.gene82311.t1.exon5;Parent=MS.gene82311.t1
chr3.4 CDS 21712888 21712937 21712888 ID=cds.MS.gene82311.t1;Parent=MS.gene82311.t1
Gene Sequence

>MS.gene82311

ATGATCTGGATTTGGCCTGGCAGTGATCCCCAAACAGCCACACTACCTTCTTTACTACCTCCTCCTGGATTTATGGTCCTTGCCGAGGTATATAATAAGTATAGTCTGTGTATATAATAAGTATAGTATGTCCTAATAGGTATTATGAGCTATGAAAGAACAGTGAATTACAATTTTATGATTATAAAGAAAAAAGAAGATAAAAGTCATGGTTTCTTACCTGGTATTTTTTCACAAAACAGATTGTCATGAAACTTCCGGTTGAACATGGCTTACTTTTTTACAACCTTTTGGATCTTGCGCACGTCCCTTTTACTCATACTTCAACATTTGCTAAGGGATGGAGTGTTCCCAGGTTTGTATCGTTTATGCCCTTTGAAGTGATATGATTCAATATGTGAAAATGAAATTGATTTAAGTTCTTCAAAATAGCCAATTTGTTATGACAAACTCTTAGAAGCCTTTTTCTTTTGACAACTTTAGGGTAAATGTATTGCAGTCCTATGTTTTTTCTGAGTTAATATATCATCATAACTTTTTTTTTAAGAAGAGGATGTATCATCATAACTTAGGGCCTGTTTGAGTTTGTTTATTTGATCATATTTACCTGGCATAAGCACTTGTGGCACTGTTTGGAAAAGTATATGGAAACAACTTATTTCCATGAGCTCTCCAAAATAGCAGTTATGTAAACAATTTGACTTTATTTTATTTCTTGTGATAAAAATAATTTATACATTACTACTTATTTGATATGCTTTTTATAATACAAGCACTTAATTAAGCTGTTTATCCTTAGATATTTCGATATGTGCGTGAAATGGTTCAAATTTTAGTTATAACTACTTTTAATATTATGAAATAATACATTTATAGATCACTTACTCTCAATTGATTCAGTAGGCGAGATCTGGTACTAAGATGTCTGCATTTTTAGTTTATTTTTTTCATATGCTTTGAAAGTTATCCATGGAATTTATTTTCTTAAAGGAAATCAAGTAGAAAGTTTTTGTTCCTATATTTTAAGATGGTCAGTTCCAAACATCTTTCTGAAATAGTTCTTTACTTTACTTTTTCTGTAGGAAGGATCCAAAGAGAATAGCTGACATCTTTAGTTCAACAGAGAATGAAGAATCCTTCACAACTTACAATATGTTGAAGGTACAATCAATCACTCATCATTCTTTTAGTTCTTCATGTAATCTTAGGGAAGTTAAAAATGTGCATCTGCTAAATTTATTATCATATGGTAGTTCATTGGACTTGAAAAAAAAGGTAGTTTATTGGTATTATGTTTATGATTTATTGTTGAATATTTGTTCCTATGTCAGTGTGATACATTAATTGATATTCAGAGATGATTCTTAGTTTATTCTGTTGAAGTATTTATGTTGCAAGATAATGATTTCAGGTTTCGCGGCACCTGTTTAGATGGACAAAAGAAGTCTCATACGCAGATTATTATGAACGTGCCTTAACGAATGGTGTACTCAGTATACAAAGAGGAACAGATCCTGGGGTGATGATTTATATGCTTCCTTTAGGTATTGGGGTTTCCAAAGCTAAAACTGGACATAGTTGGGGAAATCCAATAATGATTCTGCTGTGGATTTTTTTTTGTTTGAAATTTCTGCCTAAAGTTGTTTTATTGGATTATTTCTAGCTATATTGTATGCTTATGCCTCATTTATAGATTGGAATATTGAGGATTGA

Protein sequence

>MS.gene82311.t1

MIWIWPGSDPQTATLPSLLPPPGFMVLAEIVMKLPVEHGLLFYNLLDLAHVPFTHTSTFAKGWSVPRKDPKRIADIFSSTENEESFTTYNMLKVSRHLFRWTKEVSYADYYERALTNGVLSIQRGTDPGVMIYMLPLAILYAYASFIDWNIED