Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84540.t1 | XP_003600464.2 | 93.8 | 97 | 6 | 0 | 1 | 97 | 53 | 149 | 2.30E-38 | 167.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84540.t1 | G7IYA1 | 93.8 | 97 | 6 | 0 | 1 | 97 | 49 | 145 | 1.6e-38 | 167.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene84540 | 0.827395 | 1.60E-54 | -1.69E-46 |
| MS.gene049285 | MS.gene84540 | 0.821325 | 4.29E-53 | -1.69E-46 |
| MS.gene049320 | MS.gene84540 | 0.807422 | 5.09E-50 | -1.69E-46 |
| MS.gene049633 | MS.gene84540 | 0.808507 | 2.99E-50 | -1.69E-46 |
| MS.gene050323 | MS.gene84540 | 0.801521 | 8.66E-49 | -1.69E-46 |
| MS.gene050504 | MS.gene84540 | 0.819051 | 1.42E-52 | -1.69E-46 |
| MS.gene050577 | MS.gene84540 | 0.804814 | 1.80E-49 | -1.69E-46 |
| MS.gene050699 | MS.gene84540 | 0.802568 | 5.28E-49 | -1.69E-46 |
| MS.gene050772 | MS.gene84540 | 0.80152 | 8.66E-49 | -1.69E-46 |
| MS.gene050868 | MS.gene84540 | 0.806378 | 8.47E-50 | -1.69E-46 |
| MS.gene050871 | MS.gene84540 | 0.807351 | 5.27E-50 | -1.69E-46 |
| MS.gene050986 | MS.gene84540 | 0.810415 | 1.17E-50 | -1.69E-46 |
| MS.gene051551 | MS.gene84540 | 0.838046 | 3.63E-57 | -1.69E-46 |
| MS.gene051692 | MS.gene84540 | 0.806127 | 9.57E-50 | -1.69E-46 |
| MS.gene052503 | MS.gene84540 | 0.825563 | 4.38E-54 | -1.69E-46 |
| MS.gene052615 | MS.gene84540 | 0.836243 | 1.05E-56 | -1.69E-46 |
| MS.gene052823 | MS.gene84540 | 0.821802 | 3.33E-53 | -1.69E-46 |
| MS.gene052825 | MS.gene84540 | 0.821159 | 4.68E-53 | -1.69E-46 |
| MS.gene053213 | MS.gene84540 | 0.810056 | 1.39E-50 | -1.69E-46 |
| MS.gene053286 | MS.gene84540 | 0.806781 | 6.96E-50 | -1.69E-46 |
| MS.gene053480 | MS.gene84540 | 0.811247 | 7.70E-51 | -1.69E-46 |
| MS.gene053824 | MS.gene84540 | 0.813488 | 2.50E-51 | -1.69E-46 |
| MS.gene054408 | MS.gene84540 | 0.809482 | 1.85E-50 | -1.69E-46 |
| MS.gene05470 | MS.gene84540 | 0.811929 | 5.47E-51 | -1.69E-46 |
| MS.gene054954 | MS.gene84540 | 0.818959 | 1.49E-52 | -1.69E-46 |
| MS.gene055362 | MS.gene84540 | 0.816589 | 5.12E-52 | -1.69E-46 |
| MS.gene055502 | MS.gene84540 | 0.81388 | 2.05E-51 | -1.69E-46 |
| MS.gene05589 | MS.gene84540 | 0.824303 | 8.69E-54 | -1.69E-46 |
| MS.gene056374 | MS.gene84540 | 0.806224 | 9.12E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene84540 | 0.835344 | 1.77E-56 | -1.69E-46 |
| MS.gene056510 | MS.gene84540 | 0.812612 | 3.88E-51 | -1.69E-46 |
| MS.gene056657 | MS.gene84540 | 0.816263 | 6.05E-52 | -1.69E-46 |
| MS.gene056946 | MS.gene84540 | 0.825668 | 4.13E-54 | -1.69E-46 |
| MS.gene058419 | MS.gene84540 | 0.823169 | 1.60E-53 | -1.69E-46 |
| MS.gene058544 | MS.gene84540 | 0.800651 | 1.30E-48 | -1.69E-46 |
| MS.gene058838 | MS.gene84540 | 0.806439 | 8.22E-50 | -1.69E-46 |
| MS.gene059022 | MS.gene84540 | 0.825399 | 4.79E-54 | -1.69E-46 |
| MS.gene059023 | MS.gene84540 | 0.813443 | 2.55E-51 | -1.69E-46 |
| MS.gene059677 | MS.gene84540 | 0.812428 | 4.26E-51 | -1.69E-46 |
| MS.gene059718 | MS.gene84540 | 0.803489 | 3.40E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene84540 | 0.815638 | 8.34E-52 | -1.69E-46 |
| MS.gene060703 | MS.gene84540 | 0.819795 | 9.63E-53 | -1.69E-46 |
| MS.gene060762 | MS.gene84540 | 0.820736 | 5.86E-53 | -1.69E-46 |
| MS.gene060778 | MS.gene84540 | 0.814264 | 1.68E-51 | -1.69E-46 |
| MS.gene06077 | MS.gene84540 | 0.815554 | 8.71E-52 | -1.69E-46 |
| MS.gene060982 | MS.gene84540 | 0.81161 | 6.42E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGCTCTTTGAGGGCTTCTT+TGG | 0.197880 | 3.1:-46221139 | None:intergenic |
| AGGGCTTCTTTGGCTTTCTC+TGG | 0.231668 | 3.1:-46221129 | None:intergenic |
| CTGCATAGTTCATGCATTAA+AGG | 0.282045 | 3.1:+46221080 | None:intergenic |
| GTAATTAAGCCAGCCATTGA+TGG | 0.399846 | 3.1:+46222016 | MS.gene84540:CDS |
| TTTCTGGTCAAGCTCTTTGA+GGG | 0.426726 | 3.1:-46221148 | None:intergenic |
| GGGCTTCTTTGGCTTTCTCT+GGG | 0.431656 | 3.1:-46221128 | None:intergenic |
| ACCTTTCTCGCACTTCTAGC+TGG | 0.484342 | 3.1:+46221947 | MS.gene84540:CDS |
| ACCAGCTAGAAGTGCGAGAA+AGG | 0.498110 | 3.1:-46221948 | None:intergenic |
| TATAGGTTATGGACCATCAA+TGG | 0.534653 | 3.1:-46222029 | None:intergenic |
| TGGCTGGCTTAATTACAGTA+AGG | 0.551216 | 3.1:-46222009 | None:intergenic |
| GGTTATGGACCATCAATGGC+TGG | 0.567707 | 3.1:-46222025 | None:intergenic |
| TTTCTCGCACTTCTAGCTGG+TGG | 0.577664 | 3.1:+46221950 | MS.gene84540:CDS |
| CTTATCACCTCCTCTCTCGT+AGG | 0.590439 | 3.1:-46221893 | None:intergenic |
| AGAAATGAAGCCTACGAGAG+AGG | 0.629871 | 3.1:+46221883 | MS.gene84540:CDS |
| AATGAAGCCTACGAGAGAGG+AGG | 0.660242 | 3.1:+46221886 | MS.gene84540:CDS |
| GAGGTGATAAGTGACAACAA+TGG | 0.780630 | 3.1:+46221905 | MS.gene84540:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAACCGTAAAATTAAAAAA+GGG | - | chr3.1:46221374-46221393 | None:intergenic | 15.0% |
| !! | AAATATGTTGAATAATAACA+AGG | + | chr3.1:46221680-46221699 | MS.gene84540:intron | 15.0% |
| !! | ATTATTCAACATATTTGAAT+TGG | - | chr3.1:46221676-46221695 | None:intergenic | 15.0% |
| !!! | ATTTCCCTTTTTTAATTTTA+CGG | + | chr3.1:46221367-46221386 | MS.gene84540:intron | 15.0% |
| !!! | TTTGGAAAAAACAATTATTT+TGG | + | chr3.1:46221405-46221424 | MS.gene84540:intron | 15.0% |
| !! | AAGTATGTACTATTTATAAG+TGG | - | chr3.1:46221838-46221857 | None:intergenic | 20.0% |
| !! | ACATCATATATAGTTAATCT+TGG | - | chr3.1:46221644-46221663 | None:intergenic | 20.0% |
| !! | ACGAAATGTAAATTTAGAAA+AGG | - | chr3.1:46221589-46221608 | None:intergenic | 20.0% |
| !! | CAAAACCGTAAAATTAAAAA+AGG | - | chr3.1:46221375-46221394 | None:intergenic | 20.0% |
| !! | CAAAATAAGAGAAAAAGAAA+CGG | - | chr3.1:46221229-46221248 | None:intergenic | 20.0% |
| !! | CGAAATGTAAATTTAGAAAA+GGG | - | chr3.1:46221588-46221607 | None:intergenic | 20.0% |
| !!! | AGAGTATAATTTTCTTCATT+CGG | + | chr3.1:46221755-46221774 | MS.gene84540:intron | 20.0% |
| !!! | TTACATTTTATCTTGAGTTA+AGG | - | chr3.1:46221461-46221480 | None:intergenic | 20.0% |
| ! | AATAATAAGGTGTAAAGTCA+AGG | + | chr3.1:46221545-46221564 | MS.gene84540:intron | 25.0% |
| ! | ATAATAAGGTGTAAAGTCAA+GGG | + | chr3.1:46221546-46221565 | MS.gene84540:intron | 25.0% |
| ! | GAAGTTTATGTCTTATGATA+AGG | + | chr3.1:46221287-46221306 | MS.gene84540:intron | 25.0% |
| ! | GAATTGCAATTGATTATAGT+AGG | + | chr3.1:46221342-46221361 | MS.gene84540:intron | 25.0% |
| ! | GACTTTACACCTTATTATTA+CGG | - | chr3.1:46221544-46221563 | None:intergenic | 25.0% |
| !! | TAGAGATAGATTGAATTTTC+TGG | - | chr3.1:46221167-46221186 | None:intergenic | 25.0% |
| !! | TGTAGTGCTTTTAAATTTTG+TGG | + | chr3.1:46221265-46221284 | MS.gene84540:intron | 25.0% |
| !!! | CGGTTTTGTTTATTTCTTTT+TGG | + | chr3.1:46221387-46221406 | MS.gene84540:intron | 25.0% |
| !!! | TTCTCTTCATTTTTCATTCT+AGG | + | chr3.1:46221715-46221734 | MS.gene84540:intron | 25.0% |
| AAAATTATACTCTTCGAGTG+AGG | - | chr3.1:46221748-46221767 | None:intergenic | 30.0% | |
| AAATTATACTCTTCGAGTGA+GGG | - | chr3.1:46221747-46221766 | None:intergenic | 30.0% | |
| AATTGACCACCGTAATAATA+AGG | + | chr3.1:46221532-46221551 | MS.gene84540:intron | 30.0% | |
| ACTTGTATAATCTTAGGAGA+GGG | - | chr3.1:46221617-46221636 | None:intergenic | 30.0% | |
| TTTACACCTTATTATTACGG+TGG | - | chr3.1:46221541-46221560 | None:intergenic | 30.0% | |
| !! | TTCATTTTTCATTCTAGGAG+TGG | + | chr3.1:46221720-46221739 | MS.gene84540:intron | 30.0% |
| AGTTTCCTCTCAAAAACTCA+AGG | + | chr3.1:46221194-46221213 | MS.gene84540:CDS | 35.0% | |
| CTCAATGAACTCAAACTCTT+TGG | + | chr3.1:46221806-46221825 | MS.gene84540:intron | 35.0% | |
| CTTTGCACTCGTTGTTATAA+TGG | + | chr3.1:46221434-46221453 | MS.gene84540:intron | 35.0% | |
| GACTTGTATAATCTTAGGAG+AGG | - | chr3.1:46221618-46221637 | None:intergenic | 35.0% | |
| GTTTCCTCTCAAAAACTCAA+GGG | + | chr3.1:46221195-46221214 | MS.gene84540:CDS | 35.0% | |
| TATAACAACGAGTGCAAAGT+AGG | - | chr3.1:46221433-46221452 | None:intergenic | 35.0% | |
| TCAAAGACTAAACCGACTAA+CGG | - | chr3.1:46221506-46221525 | None:intergenic | 35.0% | |
| ! | GGCATGACTTGTATAATCTT+AGG | - | chr3.1:46221623-46221642 | None:intergenic | 35.0% |
| ACAAAATCCTTCGTCTAACG+AGG | - | chr3.1:46221313-46221332 | None:intergenic | 40.0% | |
| GAGGTGATAAGTGACAACAA+TGG | + | chr3.1:46221905-46221924 | MS.gene84540:CDS | 40.0% | |
| GTAATTAAGCCAGCCATTGA+TGG | + | chr3.1:46222016-46222035 | MS.gene84540:CDS | 40.0% | |
| TAAGGTGTAAAGTCAAGGGT+CGG | + | chr3.1:46221550-46221569 | MS.gene84540:intron | 40.0% | |
| ! | TTTCTGGTCAAGCTCTTTGA+GGG | - | chr3.1:46221151-46221170 | None:intergenic | 40.0% |
| !! | AGTACCCTTGAGTTTTTGAG+AGG | - | chr3.1:46221202-46221221 | None:intergenic | 40.0% |
| !! | TGGCTGGCTTAATTACAGTA+AGG | - | chr3.1:46222012-46222031 | None:intergenic | 40.0% |
| !! | TTTTCTGGTCAAGCTCTTTG+AGG | - | chr3.1:46221152-46221171 | None:intergenic | 40.0% |
| AGAAATGAAGCCTACGAGAG+AGG | + | chr3.1:46221883-46221902 | MS.gene84540:CDS | 45.0% | |
| AGACGTGTACATCCGTTAGT+CGG | + | chr3.1:46221491-46221510 | MS.gene84540:intron | 45.0% | |
| ! | AAGCTCTTTGAGGGCTTCTT+TGG | - | chr3.1:46221142-46221161 | None:intergenic | 45.0% |
| AATGAAGCCTACGAGAGAGG+AGG | + | chr3.1:46221886-46221905 | MS.gene84540:CDS | 50.0% | |
| ACCTTTCTCGCACTTCTAGC+TGG | + | chr3.1:46221947-46221966 | MS.gene84540:CDS | 50.0% | |
| CTTATCACCTCCTCTCTCGT+AGG | - | chr3.1:46221896-46221915 | None:intergenic | 50.0% | |
| GATAAGGCCTCGTTAGACGA+AGG | + | chr3.1:46221303-46221322 | MS.gene84540:intron | 50.0% | |
| ! | ACCAGCTAGAAGTGCGAGAA+AGG | - | chr3.1:46221951-46221970 | None:intergenic | 50.0% |
| ! | TTTCTCGCACTTCTAGCTGG+TGG | + | chr3.1:46221950-46221969 | MS.gene84540:CDS | 50.0% |
| !! | AGGGCTTCTTTGGCTTTCTC+TGG | - | chr3.1:46221132-46221151 | None:intergenic | 50.0% |
| !! | GGGCTTCTTTGGCTTTCTCT+GGG | - | chr3.1:46221131-46221150 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 46221088 | 46222045 | 46221088 | ID=MS.gene84540 |
| chr3.1 | mRNA | 46221088 | 46222045 | 46221088 | ID=MS.gene84540.t1;Parent=MS.gene84540 |
| chr3.1 | exon | 46221088 | 46221216 | 46221088 | ID=MS.gene84540.t1.exon1;Parent=MS.gene84540.t1 |
| chr3.1 | CDS | 46221088 | 46221216 | 46221088 | ID=cds.MS.gene84540.t1;Parent=MS.gene84540.t1 |
| chr3.1 | exon | 46221879 | 46222045 | 46221879 | ID=MS.gene84540.t1.exon2;Parent=MS.gene84540.t1 |
| chr3.1 | CDS | 46221879 | 46222045 | 46221879 | ID=cds.MS.gene84540.t1;Parent=MS.gene84540.t1 |
| Gene Sequence |
| Protein sequence |
