Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84833.t1 | XP_003627523.2 | 97.1 | 274 | 8 | 0 | 1 | 274 | 3 | 276 | 2.10E-145 | 525 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84833.t1 | G7LAU0 | 97.1 | 274 | 8 | 0 | 1 | 274 | 3 | 276 | 1.5e-145 | 525.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049260 | MS.gene84833 | 0.804375 | 2.23E-49 | -1.69E-46 |
| MS.gene050020 | MS.gene84833 | 0.883036 | 6.30E-71 | -1.69E-46 |
| MS.gene05016 | MS.gene84833 | 0.810356 | 1.20E-50 | -1.69E-46 |
| MS.gene050206 | MS.gene84833 | 0.842258 | 2.89E-58 | -1.69E-46 |
| MS.gene050563 | MS.gene84833 | 0.832386 | 9.74E-56 | -1.69E-46 |
| MS.gene050584 | MS.gene84833 | 0.87746 | 6.17E-69 | -1.69E-46 |
| MS.gene050585 | MS.gene84833 | 0.849175 | 3.83E-60 | -1.69E-46 |
| MS.gene050586 | MS.gene84833 | 0.838792 | 2.33E-57 | -1.69E-46 |
| MS.gene050587 | MS.gene84833 | 0.816043 | 6.78E-52 | -1.69E-46 |
| MS.gene050807 | MS.gene84833 | 0.882388 | 1.09E-70 | -1.69E-46 |
| MS.gene050808 | MS.gene84833 | 0.855521 | 5.97E-62 | -1.69E-46 |
| MS.gene05143 | MS.gene84833 | 0.814574 | 1.44E-51 | -1.69E-46 |
| MS.gene051626 | MS.gene84833 | 0.848115 | 7.53E-60 | -1.69E-46 |
| MS.gene051789 | MS.gene84833 | 0.906175 | 1.96E-80 | -1.69E-46 |
| MS.gene052142 | MS.gene84833 | 0.843849 | 1.09E-58 | -1.69E-46 |
| MS.gene052665 | MS.gene84833 | 0.824079 | 9.80E-54 | -1.69E-46 |
| MS.gene052698 | MS.gene84833 | 0.801582 | 8.41E-49 | -1.69E-46 |
| MS.gene052967 | MS.gene84833 | 0.813657 | 2.29E-51 | -1.69E-46 |
| MS.gene053074 | MS.gene84833 | 0.905209 | 5.47E-80 | -1.69E-46 |
| MS.gene053184 | MS.gene84833 | 0.806396 | 8.40E-50 | -1.69E-46 |
| MS.gene053204 | MS.gene84833 | 0.823556 | 1.30E-53 | -1.69E-46 |
| MS.gene053383 | MS.gene84833 | 0.868593 | 5.82E-66 | -1.69E-46 |
| MS.gene053563 | MS.gene84833 | 0.85051 | 1.62E-60 | -1.69E-46 |
| MS.gene053630 | MS.gene84833 | 0.815229 | 1.03E-51 | -1.69E-46 |
| MS.gene054184 | MS.gene84833 | 0.803125 | 4.05E-49 | -1.69E-46 |
| MS.gene054185 | MS.gene84833 | 0.806086 | 9.76E-50 | -1.69E-46 |
| MS.gene05456 | MS.gene84833 | 0.883959 | 2.88E-71 | -1.69E-46 |
| MS.gene054596 | MS.gene84833 | 0.852215 | 5.34E-61 | -1.69E-46 |
| MS.gene055207 | MS.gene84833 | 0.822116 | 2.81E-53 | -1.69E-46 |
| MS.gene055358 | MS.gene84833 | 0.811383 | 7.20E-51 | -1.69E-46 |
| MS.gene055359 | MS.gene84833 | 0.857787 | 1.29E-62 | -1.69E-46 |
| MS.gene056069 | MS.gene84833 | -0.815751 | 7.87E-52 | -1.69E-46 |
| MS.gene056760 | MS.gene84833 | 0.810646 | 1.04E-50 | -1.69E-46 |
| MS.gene057060 | MS.gene84833 | 0.817891 | 2.61E-52 | -1.69E-46 |
| MS.gene057395 | MS.gene84833 | 0.808159 | 3.55E-50 | -1.69E-46 |
| MS.gene058168 | MS.gene84833 | 0.833034 | 6.72E-56 | -1.69E-46 |
| MS.gene059006 | MS.gene84833 | 0.911792 | 4.07E-83 | -1.69E-46 |
| MS.gene059124 | MS.gene84833 | 0.818906 | 1.53E-52 | -1.69E-46 |
| MS.gene060470 | MS.gene84833 | 0.812162 | 4.87E-51 | -1.69E-46 |
| MS.gene060513 | MS.gene84833 | 0.873456 | 1.45E-67 | -1.69E-46 |
| MS.gene060691 | MS.gene84833 | 0.80106 | 1.08E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84833.t1 | MTR_8g027100 | 97.080 | 274 | 8 | 0 | 1 | 274 | 3 | 276 | 0.0 | 544 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84833.t1 | AT2G46640 | 32.584 | 267 | 141 | 12 | 1 | 248 | 3 | 249 | 1.46e-20 | 89.0 |
| MS.gene84833.t1 | AT2G46640 | 32.463 | 268 | 142 | 12 | 1 | 248 | 3 | 251 | 3.90e-19 | 85.1 |
| MS.gene84833.t1 | AT2G46640 | 32.463 | 268 | 142 | 12 | 1 | 248 | 3 | 251 | 4.27e-19 | 85.1 |
| MS.gene84833.t1 | AT2G46640 | 30.736 | 231 | 125 | 9 | 34 | 248 | 26 | 237 | 1.09e-14 | 72.8 |
Find 41 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCCAAAGCAAAGTATTCT+TGG | 0.268337 | 8.4:-69834637 | None:intergenic |
| GTATTCTTGGTGATTGTTCA+TGG | 0.327683 | 8.4:-69834624 | None:intergenic |
| GAAGATTATTCCCCTTGTTA+AGG | 0.356621 | 8.4:+69835063 | MS.gene84833:CDS |
| GTTTGTTACAGATCTTCAAT+TGG | 0.412010 | 8.4:+69834275 | None:intergenic |
| AATGATGATTAGAAACTTCA+AGG | 0.418804 | 8.4:+69834832 | MS.gene84833:CDS |
| TCACCAAGAATACTTTGCTT+TGG | 0.419615 | 8.4:+69834634 | MS.gene84833:CDS |
| ATTCTTACCATTGGTACTCT+TGG | 0.429438 | 8.4:+69834584 | MS.gene84833:CDS |
| ACTTGACTCAACCAAGACTT+TGG | 0.432800 | 8.4:-69834996 | None:intergenic |
| TTTGTTACAGATCTTCAATT+GGG | 0.432814 | 8.4:+69834276 | None:intergenic |
| ACTTTCTTGATGTCTTTCAT+TGG | 0.437252 | 8.4:-69835097 | None:intergenic |
| AAACCGTTGAGAGTGTTGTT+AGG | 0.437651 | 8.4:-69834316 | None:intergenic |
| ATGATTTCATGACAAATCAT+AGG | 0.438088 | 8.4:-69834872 | None:intergenic |
| TCAAATGTGTTGTTGATCAA+TGG | 0.438577 | 8.4:-69834737 | None:intergenic |
| TTTCCAATCACCGAGCATAT+CGG | 0.445673 | 8.4:-69834555 | None:intergenic |
| TGACCGATATGCTCGGTGAT+TGG | 0.446300 | 8.4:+69834552 | MS.gene84833:CDS |
| TGATTCTTGACATCAAGATC+TGG | 0.455218 | 8.4:-69835461 | None:intergenic |
| ATTTGATGAGAACAACTTTG+AGG | 0.478699 | 8.4:+69834754 | MS.gene84833:CDS |
| TTATCAGTGTTGTTGATTGA+AGG | 0.480019 | 8.4:-69834503 | None:intergenic |
| ATTAATAACCACATGAATGA+AGG | 0.483710 | 8.4:+69835503 | MS.gene84833:CDS |
| TGATGAAGAAGATGCTAAAG+AGG | 0.486614 | 8.4:+69835429 | MS.gene84833:CDS |
| TATGATTTGTCATGAAATCA+TGG | 0.489589 | 8.4:+69834874 | MS.gene84833:CDS |
| TTGAAGCTGATCAAAAGAAA+AGG | 0.490869 | 8.4:+69834909 | MS.gene84833:CDS |
| TGATGATGATGCTAGAAAAG+AGG | 0.505541 | 8.4:+69834805 | MS.gene84833:CDS |
| TTATTCTGCAGCCAAAGTCT+TGG | 0.511362 | 8.4:+69834985 | MS.gene84833:CDS |
| TTTCTATGATGTTGAACAAG+AGG | 0.511917 | 8.4:+69834706 | MS.gene84833:CDS |
| AGCCATTGCCTTCATTCATG+TGG | 0.516778 | 8.4:-69835511 | None:intergenic |
| TCTTCAATTGGGTGCACAAG+AGG | 0.532391 | 8.4:+69834287 | MS.gene84833:CDS |
| AAAGACGGAATTCTTACCAT+TGG | 0.543523 | 8.4:+69834575 | MS.gene84833:CDS |
| AAACTTCAAGGAAATTGTTG+AGG | 0.544282 | 8.4:+69834844 | MS.gene84833:CDS |
| CATCCTAACAACACTCTCAA+CGG | 0.551417 | 8.4:+69834313 | MS.gene84833:CDS |
| CTTCAAGGAAATTGTTGAGG+TGG | 0.564269 | 8.4:+69834847 | MS.gene84833:CDS |
| ATCATAGCCAAGAGTACCAA+TGG | 0.569824 | 8.4:-69834591 | None:intergenic |
| CATCATCATCAACTACATCA+TGG | 0.574853 | 8.4:-69834793 | None:intergenic |
| ATGCTCGGTGATTGGAAAGA+CGG | 0.581029 | 8.4:+69834560 | MS.gene84833:CDS |
| TCTTGACATCAAGATCTGGA+TGG | 0.589109 | 8.4:-69835457 | None:intergenic |
| TGAATGAAGGCAATGGCTCA+AGG | 0.597220 | 8.4:+69835516 | MS.gene84833:CDS |
| TGATTGTTCATGGATTTCAA+AGG | 0.604206 | 8.4:-69834614 | None:intergenic |
| AACCACATGAATGAAGGCAA+TGG | 0.612697 | 8.4:+69835509 | MS.gene84833:CDS |
| GTTGCTTTGACCGATATGCT+CGG | 0.632441 | 8.4:+69834545 | MS.gene84833:CDS |
| TCTTAACATCAGAATCAGCA+AGG | 0.653265 | 8.4:-69834952 | None:intergenic |
| GATTTGTCATGAAATCATGG+AGG | 0.748556 | 8.4:+69834877 | MS.gene84833:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATTTGCTTGAAATTAAT+TGG | + | chr8.4:69835376-69835395 | MS.gene84833:intron | 15.0% |
| !! | TCAAGCAAATAATTAATTAT+TGG | - | chr8.4:69835369-69835388 | None:intergenic | 15.0% |
| !!! | TTTATTTTAGACACAAAATT+TGG | - | chr8.4:69834388-69834407 | None:intergenic | 15.0% |
| !! | TAATTACATATATAGCCTTT+AGG | - | chr8.4:69835149-69835168 | None:intergenic | 20.0% |
| !!! | ATTTATTTCCATTTTCACAA+TGG | + | chr8.4:69835253-69835272 | MS.gene84833:intron | 20.0% |
| ! | AATGATGATTAGAAACTTCA+AGG | + | chr8.4:69834832-69834851 | MS.gene84833:CDS | 25.0% |
| ! | ATGATTTCATGACAAATCAT+AGG | - | chr8.4:69834875-69834894 | None:intergenic | 25.0% |
| ! | ATTAATAACCACATGAATGA+AGG | + | chr8.4:69835503-69835522 | MS.gene84833:CDS | 25.0% |
| ! | CCAAAACTTAAAGCAAAAAA+TGG | + | chr8.4:69835025-69835044 | MS.gene84833:CDS | 25.0% |
| ! | TTGCTTGAAATTAATTGGTT+AGG | + | chr8.4:69835381-69835400 | MS.gene84833:intron | 25.0% |
| !! | TATGATTTGTCATGAAATCA+TGG | + | chr8.4:69834874-69834893 | MS.gene84833:CDS | 25.0% |
| !!! | CCATTTTTTGCTTTAAGTTT+TGG | - | chr8.4:69835028-69835047 | None:intergenic | 25.0% |
| !!! | GTTTGTTGTTTTTACTTAGA+TGG | + | chr8.4:69834452-69834471 | MS.gene84833:intron | 25.0% |
| AAACTTCAAGGAAATTGTTG+AGG | + | chr8.4:69834844-69834863 | MS.gene84833:CDS | 30.0% | |
| ACTTTCTTGATGTCTTTCAT+TGG | - | chr8.4:69835100-69835119 | None:intergenic | 30.0% | |
| ATTTGATGAGAACAACTTTG+AGG | + | chr8.4:69834754-69834773 | MS.gene84833:CDS | 30.0% | |
| TGATTGTTCATGGATTTCAA+AGG | - | chr8.4:69834617-69834636 | None:intergenic | 30.0% | |
| TTGAAGCTGATCAAAAGAAA+AGG | + | chr8.4:69834909-69834928 | MS.gene84833:CDS | 30.0% | |
| TTTCTATGATGTTGAACAAG+AGG | + | chr8.4:69834706-69834725 | MS.gene84833:CDS | 30.0% | |
| ! | TCAAATGTGTTGTTGATCAA+TGG | - | chr8.4:69834740-69834759 | None:intergenic | 30.0% |
| ! | TTATCAGTGTTGTTGATTGA+AGG | - | chr8.4:69834506-69834525 | None:intergenic | 30.0% |
| AAAGACGGAATTCTTACCAT+TGG | + | chr8.4:69834575-69834594 | MS.gene84833:CDS | 35.0% | |
| ATTCTTACCATTGGTACTCT+TGG | + | chr8.4:69834584-69834603 | MS.gene84833:CDS | 35.0% | |
| CATCATCATCAACTACATCA+TGG | - | chr8.4:69834796-69834815 | None:intergenic | 35.0% | |
| CATTGACACCATTGTGAAAA+TGG | - | chr8.4:69835264-69835283 | None:intergenic | 35.0% | |
| GAAGATTATTCCCCTTGTTA+AGG | + | chr8.4:69835063-69835082 | MS.gene84833:CDS | 35.0% | |
| GATTTGTCATGAAATCATGG+AGG | + | chr8.4:69834877-69834896 | MS.gene84833:CDS | 35.0% | |
| TCACCAAGAATACTTTGCTT+TGG | + | chr8.4:69834634-69834653 | MS.gene84833:CDS | 35.0% | |
| TCTTAACATCAGAATCAGCA+AGG | - | chr8.4:69834955-69834974 | None:intergenic | 35.0% | |
| TGATGAAGAAGATGCTAAAG+AGG | + | chr8.4:69835429-69835448 | MS.gene84833:CDS | 35.0% | |
| TGATTCTTGACATCAAGATC+TGG | - | chr8.4:69835464-69835483 | None:intergenic | 35.0% | |
| TTGTAGATAGGATAGTGTTG+AGG | + | chr8.4:69835343-69835362 | MS.gene84833:intron | 35.0% | |
| ! | ACGTGCATTTTCCTTAACAA+GGG | - | chr8.4:69835077-69835096 | None:intergenic | 35.0% |
| ! | CTTCCAAAGCAAAGTATTCT+TGG | - | chr8.4:69834640-69834659 | None:intergenic | 35.0% |
| ! | GTATTCTTGGTGATTGTTCA+TGG | - | chr8.4:69834627-69834646 | None:intergenic | 35.0% |
| ! | TGATGATGATGCTAGAAAAG+AGG | + | chr8.4:69834805-69834824 | MS.gene84833:CDS | 35.0% |
| ! | TTTGGAACCTAAGTTGCAAA+TGG | - | chr8.4:69834370-69834389 | None:intergenic | 35.0% |
| AACCACATGAATGAAGGCAA+TGG | + | chr8.4:69835509-69835528 | MS.gene84833:CDS | 40.0% | |
| ACTTGACTCAACCAAGACTT+TGG | - | chr8.4:69834999-69835018 | None:intergenic | 40.0% | |
| ATCTACAAAAGAGTCCTCAC+TGG | - | chr8.4:69835331-69835350 | None:intergenic | 40.0% | |
| CATCCTAACAACACTCTCAA+CGG | + | chr8.4:69834313-69834332 | MS.gene84833:CDS | 40.0% | |
| CTACATCATGCACTTCCTAA+AGG | + | chr8.4:69835131-69835150 | MS.gene84833:intron | 40.0% | |
| CTTCAAGGAAATTGTTGAGG+TGG | + | chr8.4:69834847-69834866 | MS.gene84833:CDS | 40.0% | |
| TCTTGACATCAAGATCTGGA+TGG | - | chr8.4:69835460-69835479 | None:intergenic | 40.0% | |
| TTATTCTGCAGCCAAAGTCT+TGG | + | chr8.4:69834985-69835004 | MS.gene84833:CDS | 40.0% | |
| TTTCCAATCACCGAGCATAT+CGG | - | chr8.4:69834558-69834577 | None:intergenic | 40.0% | |
| ! | CGTGCATTTTCCTTAACAAG+GGG | - | chr8.4:69835076-69835095 | None:intergenic | 40.0% |
| ! | GACGTGCATTTTCCTTAACA+AGG | - | chr8.4:69835078-69835097 | None:intergenic | 40.0% |
| ! | GTGAGGACTCTTTTGTAGAT+AGG | + | chr8.4:69835331-69835350 | MS.gene84833:intron | 40.0% |
| !! | AAACCGTTGAGAGTGTTGTT+AGG | - | chr8.4:69834319-69834338 | None:intergenic | 40.0% |
| !! | ATCATAGCCAAGAGTACCAA+TGG | - | chr8.4:69834594-69834613 | None:intergenic | 40.0% |
| AGCCATTGCCTTCATTCATG+TGG | - | chr8.4:69835514-69835533 | None:intergenic | 45.0% | |
| CTTCGCTCCATTTGCAACTT+AGG | + | chr8.4:69834360-69834379 | MS.gene84833:intron | 45.0% | |
| GCTTCATAGTTTGACCAGTG+AGG | + | chr8.4:69835314-69835333 | MS.gene84833:intron | 45.0% | |
| TCTTCAATTGGGTGCACAAG+AGG | + | chr8.4:69834287-69834306 | MS.gene84833:CDS | 45.0% | |
| TGAATGAAGGCAATGGCTCA+AGG | + | chr8.4:69835516-69835535 | MS.gene84833:CDS | 45.0% | |
| ! | ATGCTCGGTGATTGGAAAGA+CGG | + | chr8.4:69834560-69834579 | MS.gene84833:CDS | 45.0% |
| ! | GTTGCTTTGACCGATATGCT+CGG | + | chr8.4:69834545-69834564 | MS.gene84833:CDS | 45.0% |
| ! | TGACCGATATGCTCGGTGAT+TGG | + | chr8.4:69834552-69834571 | MS.gene84833:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 69834286 | 69835556 | 69834286 | ID=MS.gene84833 |
| chr8.4 | mRNA | 69834286 | 69835556 | 69834286 | ID=MS.gene84833.t1;Parent=MS.gene84833 |
| chr8.4 | exon | 69834286 | 69834334 | 69834286 | ID=MS.gene84833.t1.exon1;Parent=MS.gene84833.t1 |
| chr8.4 | CDS | 69834286 | 69834334 | 69834286 | ID=cds.MS.gene84833.t1;Parent=MS.gene84833.t1 |
| chr8.4 | exon | 69834471 | 69835117 | 69834471 | ID=MS.gene84833.t1.exon2;Parent=MS.gene84833.t1 |
| chr8.4 | CDS | 69834471 | 69835117 | 69834471 | ID=cds.MS.gene84833.t1;Parent=MS.gene84833.t1 |
| chr8.4 | exon | 69835428 | 69835556 | 69835428 | ID=MS.gene84833.t1.exon3;Parent=MS.gene84833.t1 |
| chr8.4 | CDS | 69835428 | 69835556 | 69835428 | ID=cds.MS.gene84833.t1;Parent=MS.gene84833.t1 |
| Gene Sequence |
| Protein sequence |
