Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89266.t1 | XP_003623166.1 | 93.2 | 117 | 8 | 0 | 1 | 117 | 1 | 117 | 2.90E-56 | 227.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89266.t1 | G7L5L7 | 93.2 | 117 | 8 | 0 | 1 | 117 | 1 | 117 | 2.1e-56 | 227.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049222 | MS.gene89266 | -0.803754 | 3.00E-49 | -1.69E-46 |
| MS.gene050170 | MS.gene89266 | -0.805178 | 1.51E-49 | -1.69E-46 |
| MS.gene050323 | MS.gene89266 | -0.800094 | 1.69E-48 | -1.69E-46 |
| MS.gene050444 | MS.gene89266 | -0.81032 | 1.22E-50 | -1.69E-46 |
| MS.gene050868 | MS.gene89266 | -0.814718 | 1.34E-51 | -1.69E-46 |
| MS.gene050871 | MS.gene89266 | -0.809092 | 2.25E-50 | -1.69E-46 |
| MS.gene050904 | MS.gene89266 | -0.829436 | 5.15E-55 | -1.69E-46 |
| MS.gene051317 | MS.gene89266 | -0.806826 | 6.81E-50 | -1.69E-46 |
| MS.gene051632 | MS.gene89266 | 0.801579 | 8.43E-49 | -1.69E-46 |
| MS.gene051651 | MS.gene89266 | 0.805711 | 1.17E-49 | -1.69E-46 |
| MS.gene052606 | MS.gene89266 | 0.838501 | 2.77E-57 | -1.69E-46 |
| MS.gene053108 | MS.gene89266 | 0.824873 | 6.38E-54 | -1.69E-46 |
| MS.gene053421 | MS.gene89266 | 0.814002 | 1.92E-51 | -1.69E-46 |
| MS.gene054290 | MS.gene89266 | 0.801121 | 1.05E-48 | -1.69E-46 |
| MS.gene054706 | MS.gene89266 | 0.820942 | 5.25E-53 | -1.69E-46 |
| MS.gene055158 | MS.gene89266 | 0.92239 | 1.05E-88 | -1.69E-46 |
| MS.gene055159 | MS.gene89266 | 0.845978 | 2.90E-59 | -1.69E-46 |
| MS.gene055502 | MS.gene89266 | -0.805434 | 1.34E-49 | -1.69E-46 |
| MS.gene055577 | MS.gene89266 | -0.800807 | 1.21E-48 | -1.69E-46 |
| MS.gene055748 | MS.gene89266 | -0.811281 | 7.57E-51 | -1.69E-46 |
| MS.gene055750 | MS.gene89266 | -0.804554 | 2.04E-49 | -1.69E-46 |
| MS.gene055751 | MS.gene89266 | -0.80875 | 2.66E-50 | -1.69E-46 |
| MS.gene05589 | MS.gene89266 | -0.807824 | 4.19E-50 | -1.69E-46 |
| MS.gene056387 | MS.gene89266 | -0.803148 | 4.00E-49 | -1.69E-46 |
| MS.gene056504 | MS.gene89266 | -0.804875 | 1.75E-49 | -1.69E-46 |
| MS.gene056618 | MS.gene89266 | 0.808924 | 2.44E-50 | -1.69E-46 |
| MS.gene056856 | MS.gene89266 | 0.813564 | 2.40E-51 | -1.69E-46 |
| MS.gene057768 | MS.gene89266 | 0.835377 | 1.74E-56 | -1.69E-46 |
| MS.gene058158 | MS.gene89266 | 0.804183 | 2.44E-49 | -1.69E-46 |
| MS.gene058171 | MS.gene89266 | 0.804165 | 2.46E-49 | -1.69E-46 |
| MS.gene058173 | MS.gene89266 | 0.802865 | 4.58E-49 | -1.69E-46 |
| MS.gene058175 | MS.gene89266 | 0.811925 | 5.49E-51 | -1.69E-46 |
| MS.gene058334 | MS.gene89266 | 0.811474 | 6.88E-51 | -1.69E-46 |
| MS.gene058382 | MS.gene89266 | 0.812072 | 5.10E-51 | -1.69E-46 |
| MS.gene058418 | MS.gene89266 | 0.820756 | 5.80E-53 | -1.69E-46 |
| MS.gene058603 | MS.gene89266 | 0.82261 | 2.16E-53 | -1.69E-46 |
| MS.gene059526 | MS.gene89266 | 0.810549 | 1.09E-50 | -1.69E-46 |
| MS.gene05960 | MS.gene89266 | 0.83156 | 1.56E-55 | -1.69E-46 |
| MS.gene06000 | MS.gene89266 | -0.815261 | 1.01E-51 | -1.69E-46 |
| MS.gene060593 | MS.gene89266 | 0.800573 | 1.35E-48 | -1.69E-46 |
| MS.gene060762 | MS.gene89266 | -0.813836 | 2.09E-51 | -1.69E-46 |
| MS.gene060823 | MS.gene89266 | 0.805424 | 1.34E-49 | -1.69E-46 |
| MS.gene061044 | MS.gene89266 | 0.81273 | 3.66E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89266.t1 | MTR_7g065210 | 93.162 | 117 | 8 | 0 | 1 | 117 | 1 | 117 | 1.01e-78 | 227 |
| MS.gene89266.t1 | MTR_7g065160 | 88.889 | 117 | 13 | 0 | 1 | 117 | 1 | 117 | 8.18e-75 | 217 |
| MS.gene89266.t1 | MTR_8g070040 | 45.455 | 121 | 61 | 3 | 1 | 117 | 1 | 120 | 9.40e-26 | 93.6 |
| MS.gene89266.t1 | MTR_8g070015 | 42.105 | 114 | 61 | 3 | 1 | 110 | 1 | 113 | 5.03e-20 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89266.t1 | AT3G53980 | 68.421 | 95 | 29 | 1 | 23 | 117 | 21 | 114 | 2.24e-44 | 140 |
| MS.gene89266.t1 | AT3G53980 | 68.421 | 95 | 29 | 1 | 23 | 117 | 21 | 114 | 2.24e-44 | 140 |
| MS.gene89266.t1 | AT5G05960 | 58.120 | 117 | 44 | 2 | 5 | 117 | 1 | 116 | 8.88e-42 | 134 |
| MS.gene89266.t1 | AT2G37870 | 44.144 | 111 | 60 | 2 | 8 | 117 | 6 | 115 | 2.07e-25 | 92.8 |
Find 23 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTAAATGCTTCCATATC+TGG | 0.265044 | 7.1:-37111095 | None:intergenic |
| TAGCAAAGTTGCAGCGTTTA+GGG | 0.296288 | 7.1:-37111280 | None:intergenic |
| TTAGCAAAGTTGCAGCGTTT+AGG | 0.351023 | 7.1:-37111281 | None:intergenic |
| GGTATTGCAGTTCTTGTTGC+AGG | 0.361788 | 7.1:+37111026 | MS.gene89266:CDS |
| CGTCCACACTTATAACCAAT+GGG | 0.425846 | 7.1:-37111308 | None:intergenic |
| TCAAACGTTGCTAAAATGTC+TGG | 0.462464 | 7.1:+37111230 | MS.gene89266:CDS |
| AAGGGGTACTACACCAGATA+TGG | 0.470603 | 7.1:+37111082 | MS.gene89266:CDS |
| GGATAGTAACAGCAATTTGA+GGG | 0.472152 | 7.1:-37111259 | None:intergenic |
| GGGATAGTAACAGCAATTTG+AGG | 0.508534 | 7.1:-37111260 | None:intergenic |
| CGAGGCAAAACAATTTCATA+AGG | 0.511907 | 7.1:-37111001 | None:intergenic |
| GTTGAAAGTGCAGGTGAATG+TGG | 0.515102 | 7.1:+37111059 | MS.gene89266:CDS |
| AACTTTGCTAATCGCCCCAT+TGG | 0.518869 | 7.1:+37111293 | MS.gene89266:CDS |
| ACGTCCACACTTATAACCAA+TGG | 0.527913 | 7.1:-37111309 | None:intergenic |
| TCTTGTGCTGCACTTGCACA+AGG | 0.541464 | 7.1:-37111122 | None:intergenic |
| AAAGTGCAGGTGAATGTGGA+AGG | 0.549386 | 7.1:+37111063 | MS.gene89266:CDS |
| CGCCCCATTGGTTATAAGTG+TGG | 0.571913 | 7.1:+37111305 | MS.gene89266:CDS |
| TGTGATCAGACTAAGAAACT+TGG | 0.574065 | 7.1:+37111173 | MS.gene89266:CDS |
| ATTCATAGAGTTGAAAGTGC+AGG | 0.578390 | 7.1:+37111050 | MS.gene89266:CDS |
| AAAACTGCACAAAGACAACT+TGG | 0.590774 | 7.1:-37111203 | None:intergenic |
| AGTGCAGGTGAATGTGGAAG+GGG | 0.630405 | 7.1:+37111065 | MS.gene89266:CDS |
| AAGTGCAGGTGAATGTGGAA+GGG | 0.641454 | 7.1:+37111064 | MS.gene89266:CDS |
| CAAGAACTGCAATACCAACG+AGG | 0.744882 | 7.1:-37111019 | None:intergenic |
| GTCCACACTTATAACCAATG+GGG | 0.748420 | 7.1:-37111307 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATAACTTTTATATGAAAA+CGG | - | chr7.1:37111371-37111390 | None:intergenic | 10.0% |
| !!! | TTTTAACAATAAATTTTCTT+AGG | - | chr7.1:37111498-37111517 | None:intergenic | 10.0% |
| !! | ATGTAACATTAATAATTGAT+AGG | + | chr7.1:37111423-37111442 | MS.gene89266:intron | 15.0% |
| !!! | GTTTTCATATAAAAGTTATT+TGG | + | chr7.1:37111370-37111389 | MS.gene89266:intron | 15.0% |
| !! | AATGAGATTATAGATAGTTA+AGG | + | chr7.1:37111449-37111468 | MS.gene89266:intron | 20.0% |
| !! | ATGAGATTATAGATAGTTAA+GGG | + | chr7.1:37111450-37111469 | MS.gene89266:intron | 20.0% |
| ! | TTGTTAAAATCTTCCCTTTA+TGG | + | chr7.1:37111509-37111528 | MS.gene89266:intron | 25.0% |
| !!! | TAGATAGTTAAGGGTATTAT+AGG | + | chr7.1:37111459-37111478 | MS.gene89266:intron | 25.0% |
| TGCCTAAGAAAAATGTGAAA+CGG | + | chr7.1:37111556-37111575 | MS.gene89266:intron | 30.0% | |
| ! | CTACTTGTCAATTTTCTTGA+TGG | + | chr7.1:37111609-37111628 | MS.gene89266:intron | 30.0% |
| ! | TACCGTTTCACATTTTTCTT+AGG | - | chr7.1:37111561-37111580 | None:intergenic | 30.0% |
| !! | CTAGTTTCTAGTTCCATAAA+GGG | - | chr7.1:37111525-37111544 | None:intergenic | 30.0% |
| !! | TCTAGTTTCTAGTTCCATAA+AGG | - | chr7.1:37111526-37111545 | None:intergenic | 30.0% |
| AAAACTGCACAAAGACAACT+TGG | - | chr7.1:37111206-37111225 | None:intergenic | 35.0% | |
| AGCTTAAATGCTTCCATATC+TGG | - | chr7.1:37111098-37111117 | None:intergenic | 35.0% | |
| CGAGGCAAAACAATTTCATA+AGG | - | chr7.1:37111004-37111023 | None:intergenic | 35.0% | |
| GGATAGTAACAGCAATTTGA+GGG | - | chr7.1:37111262-37111281 | None:intergenic | 35.0% | |
| TAAGTAACTAACAAGTCCCT+CGG | + | chr7.1:37111331-37111350 | MS.gene89266:intron | 35.0% | |
| TCAAACGTTGCTAAAATGTC+TGG | + | chr7.1:37111230-37111249 | MS.gene89266:CDS | 35.0% | |
| TGTGATCAGACTAAGAAACT+TGG | + | chr7.1:37111173-37111192 | MS.gene89266:CDS | 35.0% | |
| ! | ATTCATAGAGTTGAAAGTGC+AGG | + | chr7.1:37111050-37111069 | MS.gene89266:CDS | 35.0% |
| !! | ATGAAATTGTTTTGCCTCGT+TGG | + | chr7.1:37111005-37111024 | MS.gene89266:CDS | 35.0% |
| ACGTCCACACTTATAACCAA+TGG | - | chr7.1:37111312-37111331 | None:intergenic | 40.0% | |
| CGTCCACACTTATAACCAAT+GGG | - | chr7.1:37111311-37111330 | None:intergenic | 40.0% | |
| GGCAACTTAAAAGAAACCGA+GGG | - | chr7.1:37111350-37111369 | None:intergenic | 40.0% | |
| GGGATAGTAACAGCAATTTG+AGG | - | chr7.1:37111263-37111282 | None:intergenic | 40.0% | |
| GTCCACACTTATAACCAATG+GGG | - | chr7.1:37111310-37111329 | None:intergenic | 40.0% | |
| TAGCAAAGTTGCAGCGTTTA+GGG | - | chr7.1:37111283-37111302 | None:intergenic | 40.0% | |
| TTAGCAAAGTTGCAGCGTTT+AGG | - | chr7.1:37111284-37111303 | None:intergenic | 40.0% | |
| AAAGTGCAGGTGAATGTGGA+AGG | + | chr7.1:37111063-37111082 | MS.gene89266:CDS | 45.0% | |
| AACTTTGCTAATCGCCCCAT+TGG | + | chr7.1:37111293-37111312 | MS.gene89266:CDS | 45.0% | |
| AAGTGCAGGTGAATGTGGAA+GGG | + | chr7.1:37111064-37111083 | MS.gene89266:CDS | 45.0% | |
| CAAGAACTGCAATACCAACG+AGG | - | chr7.1:37111022-37111041 | None:intergenic | 45.0% | |
| CGGCAACTTAAAAGAAACCG+AGG | - | chr7.1:37111351-37111370 | None:intergenic | 45.0% | |
| GGTATTGCAGTTCTTGTTGC+AGG | + | chr7.1:37111026-37111045 | MS.gene89266:CDS | 45.0% | |
| ! | GTTGAAAGTGCAGGTGAATG+TGG | + | chr7.1:37111059-37111078 | MS.gene89266:CDS | 45.0% |
| !! | AAGGGGTACTACACCAGATA+TGG | + | chr7.1:37111082-37111101 | MS.gene89266:CDS | 45.0% |
| AGTGCAGGTGAATGTGGAAG+GGG | + | chr7.1:37111065-37111084 | MS.gene89266:CDS | 50.0% | |
| TCTTGTGCTGCACTTGCACA+AGG | - | chr7.1:37111125-37111144 | None:intergenic | 50.0% | |
| ! | CGCCCCATTGGTTATAAGTG+TGG | + | chr7.1:37111305-37111324 | MS.gene89266:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 37110993 | 37111689 | 37110993 | ID=MS.gene89266 |
| chr7.1 | mRNA | 37110993 | 37111689 | 37110993 | ID=MS.gene89266.t1;Parent=MS.gene89266 |
| chr7.1 | exon | 37110993 | 37111329 | 37110993 | ID=MS.gene89266.t1.exon1;Parent=MS.gene89266.t1 |
| chr7.1 | CDS | 37110993 | 37111329 | 37110993 | ID=cds.MS.gene89266.t1;Parent=MS.gene89266.t1 |
| chr7.1 | exon | 37111673 | 37111689 | 37111673 | ID=MS.gene89266.t1.exon2;Parent=MS.gene89266.t1 |
| chr7.1 | CDS | 37111673 | 37111689 | 37111673 | ID=cds.MS.gene89266.t1;Parent=MS.gene89266.t1 |
| Gene Sequence |
| Protein sequence |
