Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96800.t1 | XP_024638446.1 | 95 | 180 | 9 | 0 | 2 | 181 | 126 | 305 | 3.50E-85 | 324.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96800.t1 | A0A072UP09 | 95.0 | 180 | 9 | 0 | 2 | 181 | 126 | 305 | 2.6e-85 | 324.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049230 | MS.gene96800 | 0.826169 | 3.14E-54 | -1.69E-46 |
| MS.gene049378 | MS.gene96800 | 0.803187 | 3.93E-49 | -1.69E-46 |
| MS.gene049424 | MS.gene96800 | 0.851162 | 1.06E-60 | -1.69E-46 |
| MS.gene049669 | MS.gene96800 | 0.807983 | 3.87E-50 | -1.69E-46 |
| MS.gene050642 | MS.gene96800 | 0.80125 | 9.84E-49 | -1.69E-46 |
| MS.gene050680 | MS.gene96800 | 0.802381 | 5.76E-49 | -1.69E-46 |
| MS.gene050987 | MS.gene96800 | 0.820384 | 7.06E-53 | -1.69E-46 |
| MS.gene051349 | MS.gene96800 | 0.820287 | 7.43E-53 | -1.69E-46 |
| MS.gene051370 | MS.gene96800 | 0.824832 | 6.52E-54 | -1.69E-46 |
| MS.gene05137 | MS.gene96800 | 0.804195 | 2.43E-49 | -1.69E-46 |
| MS.gene051983 | MS.gene96800 | 0.82286 | 1.89E-53 | -1.69E-46 |
| MS.gene052176 | MS.gene96800 | 0.841654 | 4.17E-58 | -1.69E-46 |
| MS.gene052286 | MS.gene96800 | 0.825214 | 5.30E-54 | -1.69E-46 |
| MS.gene052302 | MS.gene96800 | 0.805803 | 1.12E-49 | -1.69E-46 |
| MS.gene052309 | MS.gene96800 | -0.863351 | 2.65E-64 | -1.69E-46 |
| MS.gene052376 | MS.gene96800 | 0.806667 | 7.36E-50 | -1.69E-46 |
| MS.gene052426 | MS.gene96800 | 0.816787 | 4.62E-52 | -1.69E-46 |
| MS.gene052965 | MS.gene96800 | -0.802908 | 4.49E-49 | -1.69E-46 |
| MS.gene053006 | MS.gene96800 | 0.81886 | 1.57E-52 | -1.69E-46 |
| MS.gene053188 | MS.gene96800 | 0.820433 | 6.88E-53 | -1.69E-46 |
| MS.gene053216 | MS.gene96800 | 0.858492 | 7.94E-63 | -1.69E-46 |
| MS.gene054537 | MS.gene96800 | 0.826356 | 2.84E-54 | -1.69E-46 |
| MS.gene054930 | MS.gene96800 | 0.845198 | 4.72E-59 | -1.69E-46 |
| MS.gene05510 | MS.gene96800 | 0.814944 | 1.19E-51 | -1.69E-46 |
| MS.gene05548 | MS.gene96800 | 0.806768 | 7.01E-50 | -1.69E-46 |
| MS.gene055701 | MS.gene96800 | 0.816555 | 5.21E-52 | -1.69E-46 |
| MS.gene056506 | MS.gene96800 | 0.8129 | 3.36E-51 | -1.69E-46 |
| MS.gene056979 | MS.gene96800 | 0.825943 | 3.56E-54 | -1.69E-46 |
| MS.gene057060 | MS.gene96800 | 0.809102 | 2.23E-50 | -1.69E-46 |
| MS.gene057079 | MS.gene96800 | 0.850053 | 2.18E-60 | -1.69E-46 |
| MS.gene05761 | MS.gene96800 | 0.801013 | 1.10E-48 | -1.69E-46 |
| MS.gene05795 | MS.gene96800 | 0.856297 | 3.54E-62 | -1.69E-46 |
| MS.gene058200 | MS.gene96800 | 0.824053 | 9.94E-54 | -1.69E-46 |
| MS.gene059317 | MS.gene96800 | 0.816622 | 5.03E-52 | -1.69E-46 |
| MS.gene059710 | MS.gene96800 | 0.811838 | 5.73E-51 | -1.69E-46 |
| MS.gene059899 | MS.gene96800 | 0.812638 | 3.83E-51 | -1.69E-46 |
| MS.gene060918 | MS.gene96800 | 0.807493 | 4.92E-50 | -1.69E-46 |
| MS.gene061040 | MS.gene96800 | -0.834656 | 2.64E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96800.t1 | MTR_4g081550 | 95.000 | 180 | 9 | 0 | 2 | 181 | 126 | 305 | 2.83e-111 | 319 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96800.t1 | AT5G54540 | 33.125 | 160 | 89 | 3 | 28 | 181 | 150 | 297 | 1.50e-17 | 78.6 |
Find 37 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGACAGCATTCCATATGTT+TGG | 0.317466 | 4.3:-28542240 | None:intergenic |
| TGCTGATGGAGGCAGTTTCA+TGG | 0.399854 | 4.3:+28542638 | MS.gene96800:CDS |
| TTTCATGGGATTGGCAGTTA+TGG | 0.406291 | 4.3:+28542686 | MS.gene96800:CDS |
| TGACAGCATTCCATATGTTT+GGG | 0.407069 | 4.3:-28542239 | None:intergenic |
| ATTGGCAGTTATGGTGATAA+TGG | 0.408540 | 4.3:+28542695 | MS.gene96800:CDS |
| AGCCTCAACAAGACAATCCT+TGG | 0.412702 | 4.3:-28541965 | None:intergenic |
| TTCTTCGAATCACAGCTTGC+AGG | 0.414030 | 4.3:+28542285 | MS.gene96800:CDS |
| ATTTGTCTTTCTCAACTGTT+GGG | 0.421154 | 4.3:-28542605 | None:intergenic |
| GATTTGTCTTTCTCAACTGT+TGG | 0.425812 | 4.3:-28542606 | None:intergenic |
| TGAGGCTATTTCAAATCCTT+CGG | 0.427999 | 4.3:+28541981 | MS.gene96800:CDS |
| GAAGCTGACAGTTACTGAAA+TGG | 0.440322 | 4.3:+28542548 | MS.gene96800:CDS |
| TTGCTTGTGACCCAAACATA+TGG | 0.441449 | 4.3:+28542229 | MS.gene96800:CDS |
| GGGAGGAAGTTTCATGGGAT+TGG | 0.442750 | 4.3:+28542677 | MS.gene96800:CDS |
| GCAACTGCAGATTCAACAAA+TGG | 0.455044 | 4.3:+28542420 | MS.gene96800:intron |
| TAGCTCAAGTGTTGAGGCCC+AGG | 0.460395 | 4.3:+28542038 | MS.gene96800:CDS |
| ACATTTCCTGACTGAGACTC+AGG | 0.469584 | 4.3:-28542495 | None:intergenic |
| ATCTCATGGTAGTGAAGTCT+TGG | 0.480550 | 4.3:+28541927 | MS.gene96800:CDS |
| TCCCAAGGATTGTCTTGTTG+AGG | 0.484050 | 4.3:+28541963 | MS.gene96800:CDS |
| TATGTTTGGGTCACAAGCAA+TGG | 0.494032 | 4.3:-28542226 | None:intergenic |
| TGTCGTGGGAGGAAGTTTCA+TGG | 0.507727 | 4.3:+28542671 | MS.gene96800:CDS |
| GGTTCCGTCTCCTTCTCCCA+AGG | 0.509750 | 4.3:+28541948 | MS.gene96800:CDS |
| AAATCTTCAGCTGATGCTGA+TGG | 0.518948 | 4.3:+28542624 | MS.gene96800:CDS |
| AAGCAACTGAAAAGCGTGAA+TGG | 0.519901 | 4.3:-28542016 | None:intergenic |
| GAAAAGCCTGAGTCTCAGTC+AGG | 0.524090 | 4.3:+28542489 | MS.gene96800:CDS |
| GTTGCTTAGCTCAAGTGTTG+AGG | 0.535470 | 4.3:+28542032 | MS.gene96800:CDS |
| GCCTCAACAAGACAATCCTT+GGG | 0.548704 | 4.3:-28541964 | None:intergenic |
| AAAGCGTGAATGGCATGCTT+AGG | 0.550944 | 4.3:-28542006 | None:intergenic |
| GTCGTGGGAGGAAGTTTCAT+GGG | 0.556777 | 4.3:+28542672 | MS.gene96800:CDS |
| TCGAAGAAACTAGTCACTGC+AGG | 0.567121 | 4.3:-28542270 | None:intergenic |
| ATGGATTATAAGAATGTCGT+GGG | 0.569424 | 4.3:+28542657 | MS.gene96800:CDS |
| CATGGATTATAAGAATGTCG+TGG | 0.578660 | 4.3:+28542656 | MS.gene96800:CDS |
| CAAATTGAGAACTCATCTCA+TGG | 0.582375 | 4.3:+28541913 | MS.gene96800:intron |
| ACAAGACAATCCTTGGGAGA+AGG | 0.590276 | 4.3:-28541958 | None:intergenic |
| CAATCCTTGGGAGAAGGAGA+CGG | 0.611104 | 4.3:-28541952 | None:intergenic |
| TCTTCAGCTGATGCTGATGG+AGG | 0.613380 | 4.3:+28542627 | MS.gene96800:CDS |
| ATGGCATGCTTAGGCACCGA+AGG | 0.637271 | 4.3:-28541997 | None:intergenic |
| GATTATAAGAATGTCGTGGG+AGG | 0.659415 | 4.3:+28542660 | MS.gene96800:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGATAAAAACACAAAAAAAG+TGG | - | chr4.3:28542355-28542374 | None:intergenic | 20.0% |
| !! | ATCATAAAATATAACCTATC+AGG | - | chr4.3:28541769-28541788 | None:intergenic | 20.0% |
| !!! | AGGAAATGTTTTTGATTTTA+TGG | + | chr4.3:28542509-28542528 | MS.gene96800:CDS | 20.0% |
| !!! | GAATATGTTTTGAAAGAAAT+TGG | - | chr4.3:28542586-28542605 | None:intergenic | 20.0% |
| !!! | GGAAATGTTTTTGATTTTAT+GGG | + | chr4.3:28542510-28542529 | MS.gene96800:CDS | 20.0% |
| ! | AACAAATCAAAATACTCAGA+TGG | - | chr4.3:28541730-28541749 | None:intergenic | 25.0% |
| ! | ATCTGAATATGTTTATGCTT+GGG | + | chr4.3:28542143-28542162 | MS.gene96800:intron | 25.0% |
| ! | GGTTAATTTCTCAGTAAATT+TGG | + | chr4.3:28541681-28541700 | MS.gene96800:intron | 25.0% |
| ! | TACTTATTATGTGTTTATCC+TGG | + | chr4.3:28541800-28541819 | MS.gene96800:intron | 25.0% |
| ! | TATCTGAATATGTTTATGCT+TGG | + | chr4.3:28542142-28542161 | MS.gene96800:intron | 25.0% |
| ! | TCTAATCTAGATACGAAATA+AGG | - | chr4.3:28542071-28542090 | None:intergenic | 25.0% |
| ATTTGTCTTTCTCAACTGTT+GGG | - | chr4.3:28542608-28542627 | None:intergenic | 30.0% | |
| CACAAAAAAAGTGGATACAA+AGG | - | chr4.3:28542346-28542365 | None:intergenic | 30.0% | |
| TTATTATGTGTTTATCCTGG+TGG | + | chr4.3:28541803-28541822 | MS.gene96800:intron | 30.0% | |
| TTCTCAATTTGAGAATTAGC+AGG | - | chr4.3:28541904-28541923 | None:intergenic | 30.0% | |
| ! | ATGGATTATAAGAATGTCGT+GGG | + | chr4.3:28542657-28542676 | MS.gene96800:CDS | 30.0% |
| !! | TGTGTTTTTATCTTTTGTGC+TGG | + | chr4.3:28542362-28542381 | MS.gene96800:intron | 30.0% |
| AAAAGTGGATACAAAGGACA+TGG | - | chr4.3:28542340-28542359 | None:intergenic | 35.0% | |
| ACGAAATAAGGTATGTTACC+TGG | - | chr4.3:28542059-28542078 | None:intergenic | 35.0% | |
| ATGACAGCATTCCATATGTT+TGG | - | chr4.3:28542243-28542262 | None:intergenic | 35.0% | |
| ATTGGCAGTTATGGTGATAA+TGG | + | chr4.3:28542695-28542714 | MS.gene96800:CDS | 35.0% | |
| CAAATTGAGAACTCATCTCA+TGG | + | chr4.3:28541913-28541932 | MS.gene96800:intron | 35.0% | |
| CGAAATAAGGTATGTTACCT+GGG | - | chr4.3:28542058-28542077 | None:intergenic | 35.0% | |
| GATTTGTCTTTCTCAACTGT+TGG | - | chr4.3:28542609-28542628 | None:intergenic | 35.0% | |
| TGACAGCATTCCATATGTTT+GGG | - | chr4.3:28542242-28542261 | None:intergenic | 35.0% | |
| TGATGAAGTTATTGCCTGAT+AGG | + | chr4.3:28541752-28541771 | MS.gene96800:intron | 35.0% | |
| TGTAGAAACTCCTGAAAAAG+AGG | + | chr4.3:28542467-28542486 | MS.gene96800:CDS | 35.0% | |
| TTTGAGAATTAGCAGGTGAA+AGG | - | chr4.3:28541897-28541916 | None:intergenic | 35.0% | |
| ! | ATAAGAGACTGGTTGGATAA+TGG | - | chr4.3:28542314-28542333 | None:intergenic | 35.0% |
| ! | CATGGATTATAAGAATGTCG+TGG | + | chr4.3:28542656-28542675 | MS.gene96800:CDS | 35.0% |
| ! | GCTGGATTTTCATGCAAATT+GGG | + | chr4.3:28542380-28542399 | MS.gene96800:intron | 35.0% |
| ! | TGAGGCTATTTCAAATCCTT+CGG | + | chr4.3:28541981-28542000 | MS.gene96800:CDS | 35.0% |
| ! | TGCTGGATTTTCATGCAAAT+TGG | + | chr4.3:28542379-28542398 | MS.gene96800:intron | 35.0% |
| !! | TTTATCCTGGTGGATTATCT+TGG | + | chr4.3:28541813-28541832 | MS.gene96800:intron | 35.0% |
| AAATCTTCAGCTGATGCTGA+TGG | + | chr4.3:28542624-28542643 | MS.gene96800:CDS | 40.0% | |
| ATGGAAGATAAGAGACTGGT+TGG | - | chr4.3:28542321-28542340 | None:intergenic | 40.0% | |
| GAAGCTGACAGTTACTGAAA+TGG | + | chr4.3:28542548-28542567 | MS.gene96800:CDS | 40.0% | |
| GATTATAAGAATGTCGTGGG+AGG | + | chr4.3:28542660-28542679 | MS.gene96800:CDS | 40.0% | |
| GCAACTGCAGATTCAACAAA+TGG | + | chr4.3:28542420-28542439 | MS.gene96800:intron | 40.0% | |
| TATGTTTGGGTCACAAGCAA+TGG | - | chr4.3:28542229-28542248 | None:intergenic | 40.0% | |
| TTGCTTGTGACCCAAACATA+TGG | + | chr4.3:28542229-28542248 | MS.gene96800:CDS | 40.0% | |
| ! | AAGCAACTGAAAAGCGTGAA+TGG | - | chr4.3:28542019-28542038 | None:intergenic | 40.0% |
| ! | ATCTCATGGTAGTGAAGTCT+TGG | + | chr4.3:28541927-28541946 | MS.gene96800:CDS | 40.0% |
| ! | TCAGGCTTTTCCTCTTTTTC+AGG | - | chr4.3:28542480-28542499 | None:intergenic | 40.0% |
| ! | TCATGCAAATTGGGTTGCAA+TGG | + | chr4.3:28542389-28542408 | MS.gene96800:intron | 40.0% |
| ! | TTTCATGGGATTGGCAGTTA+TGG | + | chr4.3:28542686-28542705 | MS.gene96800:CDS | 40.0% |
| AAAGCGTGAATGGCATGCTT+AGG | - | chr4.3:28542009-28542028 | None:intergenic | 45.0% | |
| ACAAGACAATCCTTGGGAGA+AGG | - | chr4.3:28541961-28541980 | None:intergenic | 45.0% | |
| ACATTTCCTGACTGAGACTC+AGG | - | chr4.3:28542498-28542517 | None:intergenic | 45.0% | |
| AGCCTCAACAAGACAATCCT+TGG | - | chr4.3:28541968-28541987 | None:intergenic | 45.0% | |
| CAGATCCAAGATAATCCACC+AGG | - | chr4.3:28541821-28541840 | None:intergenic | 45.0% | |
| GCCTCAACAAGACAATCCTT+GGG | - | chr4.3:28541967-28541986 | None:intergenic | 45.0% | |
| GGACATGGAAGATAAGAGAC+TGG | - | chr4.3:28542325-28542344 | None:intergenic | 45.0% | |
| TCGAAGAAACTAGTCACTGC+AGG | - | chr4.3:28542273-28542292 | None:intergenic | 45.0% | |
| TTCTTCGAATCACAGCTTGC+AGG | + | chr4.3:28542285-28542304 | MS.gene96800:CDS | 45.0% | |
| ! | GTTGCTTAGCTCAAGTGTTG+AGG | + | chr4.3:28542032-28542051 | MS.gene96800:CDS | 45.0% |
| ! | TCCCAAGGATTGTCTTGTTG+AGG | + | chr4.3:28541963-28541982 | MS.gene96800:CDS | 45.0% |
| !! | CAATGGATTGCACAACAGTC+TGG | - | chr4.3:28542212-28542231 | None:intergenic | 45.0% |
| !!! | ACAAATGGTGCTGCTTTTGC+TGG | + | chr4.3:28542435-28542454 | MS.gene96800:CDS | 45.0% |
| CAATCCTTGGGAGAAGGAGA+CGG | - | chr4.3:28541955-28541974 | None:intergenic | 50.0% | |
| GAAAAGCCTGAGTCTCAGTC+AGG | + | chr4.3:28542489-28542508 | MS.gene96800:CDS | 50.0% | |
| GGGAGGAAGTTTCATGGGAT+TGG | + | chr4.3:28542677-28542696 | MS.gene96800:CDS | 50.0% | |
| GTCGTGGGAGGAAGTTTCAT+GGG | + | chr4.3:28542672-28542691 | MS.gene96800:CDS | 50.0% | |
| TCTTCAGCTGATGCTGATGG+AGG | + | chr4.3:28542627-28542646 | MS.gene96800:CDS | 50.0% | |
| TGCTGATGGAGGCAGTTTCA+TGG | + | chr4.3:28542638-28542657 | MS.gene96800:CDS | 50.0% | |
| TGTCGTGGGAGGAAGTTTCA+TGG | + | chr4.3:28542671-28542690 | MS.gene96800:CDS | 50.0% | |
| !! | ATGGCATGCTTAGGCACCGA+AGG | - | chr4.3:28542000-28542019 | None:intergenic | 55.0% |
| !! | TAGCTCAAGTGTTGAGGCCC+AGG | + | chr4.3:28542038-28542057 | MS.gene96800:CDS | 55.0% |
| !!! | GGTGCTGCTTTTGCTGGTTC+TGG | + | chr4.3:28542441-28542460 | MS.gene96800:CDS | 55.0% |
| GGTTCCGTCTCCTTCTCCCA+AGG | + | chr4.3:28541948-28541967 | MS.gene96800:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 28541679 | 28542740 | 28541679 | ID=MS.gene96800 |
| chr4.3 | mRNA | 28541679 | 28542740 | 28541679 | ID=MS.gene96800.t1;Parent=MS.gene96800 |
| chr4.3 | exon | 28541679 | 28541681 | 28541679 | ID=MS.gene96800.t1.exon1;Parent=MS.gene96800.t1 |
| chr4.3 | CDS | 28541679 | 28541681 | 28541679 | ID=cds.MS.gene96800.t1;Parent=MS.gene96800.t1 |
| chr4.3 | exon | 28541922 | 28542059 | 28541922 | ID=MS.gene96800.t1.exon2;Parent=MS.gene96800.t1 |
| chr4.3 | CDS | 28541922 | 28542059 | 28541922 | ID=cds.MS.gene96800.t1;Parent=MS.gene96800.t1 |
| chr4.3 | exon | 28542213 | 28542306 | 28542213 | ID=MS.gene96800.t1.exon3;Parent=MS.gene96800.t1 |
| chr4.3 | CDS | 28542213 | 28542306 | 28542213 | ID=cds.MS.gene96800.t1;Parent=MS.gene96800.t1 |
| chr4.3 | exon | 28542430 | 28542740 | 28542430 | ID=MS.gene96800.t1.exon4;Parent=MS.gene96800.t1 |
| chr4.3 | CDS | 28542430 | 28542740 | 28542430 | ID=cds.MS.gene96800.t1;Parent=MS.gene96800.t1 |
| Gene Sequence |
| Protein sequence |
