Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051370.t1 | RHN48636.1 | 84.2 | 158 | 2 | 2 | 1 | 135 | 299 | 456 | 2.70E-66 | 261.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051370.t1 | Q9SD53 | 31.2 | 157 | 80 | 3 | 2 | 134 | 308 | 460 | 1.4e-18 | 94.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051370.t1 | A0A396H5T2 | 84.2 | 158 | 2 | 2 | 1 | 135 | 299 | 456 | 2.0e-66 | 261.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050605 | MS.gene051370 | 0.820885 | 5.41E-53 | -1.69E-46 |
| MS.gene050680 | MS.gene051370 | 0.802646 | 5.08E-49 | -1.69E-46 |
| MS.gene050953 | MS.gene051370 | 0.801167 | 1.02E-48 | -1.69E-46 |
| MS.gene051368 | MS.gene051370 | 0.812874 | 3.40E-51 | -1.69E-46 |
| MS.gene051370 | MS.gene051373 | 0.855172 | 7.54E-62 | -1.69E-46 |
| MS.gene051370 | MS.gene051983 | 0.800575 | 1.35E-48 | -1.69E-46 |
| MS.gene051370 | MS.gene052426 | 0.814208 | 1.73E-51 | -1.69E-46 |
| MS.gene051370 | MS.gene054405 | 0.800117 | 1.68E-48 | -1.69E-46 |
| MS.gene051370 | MS.gene05510 | 0.814892 | 1.22E-51 | -1.69E-46 |
| MS.gene051370 | MS.gene055701 | 0.834631 | 2.68E-56 | -1.69E-46 |
| MS.gene051370 | MS.gene056506 | 0.846053 | 2.77E-59 | -1.69E-46 |
| MS.gene051370 | MS.gene056979 | 0.808303 | 3.31E-50 | -1.69E-46 |
| MS.gene051370 | MS.gene059550 | 0.807135 | 5.86E-50 | -1.69E-46 |
| MS.gene051370 | MS.gene060011 | 0.803086 | 4.12E-49 | -1.69E-46 |
| MS.gene051370 | MS.gene060310 | 0.801461 | 8.91E-49 | -1.69E-46 |
| MS.gene051370 | MS.gene060683 | 0.801338 | 9.44E-49 | -1.69E-46 |
| MS.gene051370 | MS.gene062738 | 0.80656 | 7.75E-50 | -1.69E-46 |
| MS.gene051370 | MS.gene069555 | 0.81867 | 1.74E-52 | -1.69E-46 |
| MS.gene051370 | MS.gene071884 | 0.818126 | 2.31E-52 | -1.69E-46 |
| MS.gene051370 | MS.gene073008 | 0.802417 | 5.67E-49 | -1.69E-46 |
| MS.gene051370 | MS.gene07344 | 0.803605 | 3.22E-49 | -1.69E-46 |
| MS.gene051370 | MS.gene074393 | -0.811267 | 7.62E-51 | -1.69E-46 |
| MS.gene051370 | MS.gene08200 | 0.808477 | 3.04E-50 | -1.69E-46 |
| MS.gene051370 | MS.gene08782 | 0.812511 | 4.09E-51 | -1.69E-46 |
| MS.gene051370 | MS.gene09952 | 0.810049 | 1.40E-50 | -1.69E-46 |
| MS.gene051370 | MS.gene20184 | 0.802169 | 6.37E-49 | -1.69E-46 |
| MS.gene051370 | MS.gene21149 | 0.804833 | 1.79E-49 | -1.69E-46 |
| MS.gene051370 | MS.gene22691 | 0.816513 | 5.32E-52 | -1.69E-46 |
| MS.gene051370 | MS.gene22833 | 0.815929 | 7.19E-52 | -1.69E-46 |
| MS.gene051370 | MS.gene23945 | 0.810454 | 1.14E-50 | -1.69E-46 |
| MS.gene051370 | MS.gene24337 | 0.823966 | 1.04E-53 | -1.69E-46 |
| MS.gene051370 | MS.gene25201 | 0.835925 | 1.26E-56 | -1.69E-46 |
| MS.gene051370 | MS.gene25913 | -0.814703 | 1.35E-51 | -1.69E-46 |
| MS.gene051370 | MS.gene28498 | 0.824923 | 6.20E-54 | -1.69E-46 |
| MS.gene051370 | MS.gene31283 | 0.817654 | 2.95E-52 | -1.69E-46 |
| MS.gene051370 | MS.gene39430 | 0.804768 | 1.84E-49 | -1.69E-46 |
| MS.gene051370 | MS.gene40031 | 0.80167 | 8.07E-49 | -1.69E-46 |
| MS.gene051370 | MS.gene40946 | 0.807515 | 4.87E-50 | -1.69E-46 |
| MS.gene051370 | MS.gene41734 | 0.809414 | 1.91E-50 | -1.69E-46 |
| MS.gene051370 | MS.gene43459 | 0.812275 | 4.60E-51 | -1.69E-46 |
| MS.gene051370 | MS.gene44603 | 0.803459 | 3.45E-49 | -1.69E-46 |
| MS.gene051370 | MS.gene45422 | 0.801484 | 8.81E-49 | -1.69E-46 |
| MS.gene051370 | MS.gene48723 | 0.804843 | 1.78E-49 | -1.69E-46 |
| MS.gene051370 | MS.gene49940 | 0.836161 | 1.10E-56 | -1.69E-46 |
| MS.gene051370 | MS.gene52059 | 0.81675 | 4.71E-52 | -1.69E-46 |
| MS.gene051370 | MS.gene52448 | 0.81045 | 1.15E-50 | -1.69E-46 |
| MS.gene051370 | MS.gene56431 | 0.805652 | 1.20E-49 | -1.69E-46 |
| MS.gene051370 | MS.gene56840 | 0.809044 | 2.30E-50 | -1.69E-46 |
| MS.gene051370 | MS.gene57183 | 0.837786 | 4.23E-57 | -1.69E-46 |
| MS.gene051370 | MS.gene57951 | 0.80775 | 4.34E-50 | -1.69E-46 |
| MS.gene051370 | MS.gene58503 | 0.807717 | 4.41E-50 | -1.69E-46 |
| MS.gene051370 | MS.gene61139 | 0.805462 | 1.32E-49 | -1.69E-46 |
| MS.gene051370 | MS.gene61435 | 0.811448 | 6.97E-51 | -1.69E-46 |
| MS.gene051370 | MS.gene63412 | 0.806369 | 8.50E-50 | -1.69E-46 |
| MS.gene051370 | MS.gene65895 | 0.832584 | 8.70E-56 | -1.69E-46 |
| MS.gene051370 | MS.gene65896 | 0.807551 | 4.78E-50 | -1.69E-46 |
| MS.gene051370 | MS.gene67316 | 0.825084 | 5.68E-54 | -1.69E-46 |
| MS.gene051370 | MS.gene70586 | -0.811865 | 5.65E-51 | -1.69E-46 |
| MS.gene051370 | MS.gene71871 | 0.805151 | 1.53E-49 | -1.69E-46 |
| MS.gene051370 | MS.gene72146 | 0.809446 | 1.88E-50 | -1.69E-46 |
| MS.gene051370 | MS.gene73820 | 0.820579 | 6.37E-53 | -1.69E-46 |
| MS.gene051370 | MS.gene75626 | 0.803795 | 2.94E-49 | -1.69E-46 |
| MS.gene051370 | MS.gene77579 | 0.803196 | 3.91E-49 | -1.69E-46 |
| MS.gene051370 | MS.gene78234 | 0.800971 | 1.12E-48 | -1.69E-46 |
| MS.gene051370 | MS.gene79884 | 0.800366 | 1.49E-48 | -1.69E-46 |
| MS.gene051370 | MS.gene83082 | 0.838223 | 3.27E-57 | -1.69E-46 |
| MS.gene051370 | MS.gene85663 | 0.811962 | 5.38E-51 | -1.69E-46 |
| MS.gene051370 | MS.gene87669 | 0.802732 | 4.88E-49 | -1.69E-46 |
| MS.gene051370 | MS.gene89511 | 0.805537 | 1.27E-49 | -1.69E-46 |
| MS.gene051370 | MS.gene93041 | 0.831122 | 2.00E-55 | -1.69E-46 |
| MS.gene051370 | MS.gene93875 | 0.836092 | 1.15E-56 | -1.69E-46 |
| MS.gene051370 | MS.gene95070 | 0.803397 | 3.55E-49 | -1.69E-46 |
| MS.gene051370 | MS.gene95377 | 0.803347 | 3.64E-49 | -1.69E-46 |
| MS.gene051370 | MS.gene95697 | 0.810949 | 8.94E-51 | -1.69E-46 |
| MS.gene051370 | MS.gene96800 | 0.824832 | 6.52E-54 | -1.69E-46 |
| MS.gene051370 | MS.gene98959 | 0.836822 | 7.47E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051370.t1 | MTR_5g016570 | 34.615 | 156 | 75 | 5 | 1 | 133 | 379 | 530 | 1.09e-19 | 84.0 |
| MS.gene051370.t1 | MTR_5g086340 | 28.662 | 157 | 86 | 5 | 1 | 135 | 286 | 438 | 1.52e-16 | 75.1 |
| MS.gene051370.t1 | MTR_2g035640 | 30.405 | 148 | 74 | 4 | 1 | 124 | 201 | 343 | 1.76e-11 | 60.5 |
| MS.gene051370.t1 | MTR_3g079390 | 31.973 | 147 | 73 | 4 | 1 | 124 | 302 | 444 | 2.58e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051370.t1 | AT2G36430 | 51.923 | 156 | 48 | 3 | 1 | 133 | 295 | 446 | 1.97e-41 | 142 |
| MS.gene051370.t1 | AT5G22550 | 42.308 | 156 | 63 | 3 | 1 | 133 | 286 | 437 | 7.27e-37 | 130 |
| MS.gene051370.t1 | AT5G22550 | 42.308 | 156 | 63 | 3 | 1 | 133 | 335 | 486 | 2.63e-36 | 130 |
| MS.gene051370.t1 | AT5G22560 | 41.667 | 156 | 64 | 3 | 1 | 133 | 362 | 513 | 2.90e-34 | 124 |
| MS.gene051370.t1 | AT3G44710 | 40.000 | 155 | 67 | 3 | 2 | 133 | 349 | 500 | 3.41e-34 | 124 |
| MS.gene051370.t1 | AT5G22540 | 39.103 | 156 | 68 | 3 | 1 | 133 | 283 | 434 | 2.61e-26 | 102 |
| MS.gene051370.t1 | AT3G47200 | 31.013 | 158 | 81 | 3 | 1 | 134 | 307 | 460 | 2.59e-25 | 99.8 |
| MS.gene051370.t1 | AT3G47200 | 31.013 | 158 | 81 | 3 | 1 | 134 | 307 | 460 | 2.59e-25 | 99.8 |
| MS.gene051370.t1 | AT3G50120 | 30.769 | 156 | 81 | 4 | 1 | 133 | 376 | 527 | 1.00e-22 | 92.8 |
| MS.gene051370.t1 | AT3G50160 | 32.051 | 156 | 79 | 4 | 1 | 133 | 346 | 497 | 1.69e-22 | 92.0 |
| MS.gene051370.t1 | AT3G50140 | 32.051 | 156 | 79 | 4 | 1 | 133 | 384 | 535 | 2.94e-22 | 91.3 |
| MS.gene051370.t1 | AT3G47250 | 29.936 | 157 | 82 | 3 | 1 | 133 | 324 | 476 | 5.79e-22 | 90.5 |
| MS.gene051370.t1 | AT3G47250 | 29.936 | 157 | 82 | 3 | 1 | 133 | 324 | 476 | 5.79e-22 | 90.5 |
| MS.gene051370.t1 | AT3G47250 | 29.936 | 157 | 82 | 3 | 1 | 133 | 324 | 476 | 5.79e-22 | 90.5 |
| MS.gene051370.t1 | AT3G50150 | 32.692 | 156 | 78 | 4 | 1 | 133 | 354 | 505 | 1.53e-20 | 86.7 |
| MS.gene051370.t1 | AT3G50180 | 31.677 | 161 | 79 | 4 | 1 | 134 | 429 | 585 | 3.48e-20 | 85.5 |
| MS.gene051370.t1 | AT3G50170 | 31.410 | 156 | 80 | 5 | 1 | 133 | 384 | 535 | 9.68e-20 | 84.3 |
| MS.gene051370.t1 | AT3G50130 | 33.758 | 157 | 77 | 3 | 1 | 134 | 409 | 561 | 1.21e-15 | 72.4 |
| MS.gene051370.t1 | AT3G50190 | 27.950 | 161 | 84 | 5 | 1 | 134 | 335 | 490 | 3.06e-14 | 68.6 |
| MS.gene051370.t1 | AT3G47210 | 29.688 | 128 | 68 | 3 | 1 | 110 | 342 | 465 | 4.06e-13 | 65.5 |
| MS.gene051370.t1 | AT5G11290 | 29.655 | 145 | 76 | 5 | 1 | 124 | 275 | 414 | 6.23e-11 | 58.9 |
| MS.gene051370.t1 | AT3G44695 | 30.189 | 159 | 82 | 4 | 2 | 135 | 56 | 210 | 8.19e-11 | 57.8 |
Find 18 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAACATAGTGAGAATCATTT+TGG | 0.181040 | 7.2:-8732596 | MS.gene051370:intron |
| TTGTTTGATCAATACTTATA+GGG | 0.300471 | 7.2:-8732640 | MS.gene051370:CDS |
| AAATCATCCATTGTTATTGT+TGG | 0.351944 | 7.2:+8732765 | None:intergenic |
| TTGGATATGTGTTATTTGTC+TGG | 0.356356 | 7.2:-8732515 | MS.gene051370:CDS |
| ATTGTTTGATCAATACTTAT+AGG | 0.359960 | 7.2:-8732641 | MS.gene051370:CDS |
| AAAAGATGTTGCGGTTGATT+TGG | 0.372070 | 7.2:-8732534 | MS.gene051370:CDS |
| TTGGTCGTTTATCTCTGCTT+TGG | 0.402449 | 7.2:-8732409 | MS.gene051370:CDS |
| TATCTCTGCTTTGGCTGCAT+TGG | 0.413002 | 7.2:-8732400 | MS.gene051370:CDS |
| GGAAATAGTTGGATGTTCAA+TGG | 0.418305 | 7.2:-8732464 | MS.gene051370:intron |
| GAAATAGTTGGATGTTCAAT+GGG | 0.445517 | 7.2:-8732463 | MS.gene051370:intron |
| GCACATTTGATTAATAATGC+TGG | 0.449997 | 7.2:-8732557 | MS.gene051370:CDS |
| GTCTTGCTGATTCTCACGGT+TGG | 0.468434 | 7.2:-8732378 | MS.gene051370:CDS |
| ATTGGTCTTGCTGATTCTCA+CGG | 0.512389 | 7.2:-8732382 | MS.gene051370:CDS |
| ACAATCTAATAATGTTACAT+AGG | 0.526845 | 7.2:+8732659 | None:intergenic |
| TGAAATGCCAACAATAACAA+TGG | 0.596606 | 7.2:-8732772 | MS.gene051370:CDS |
| TTGAATGTGAAGTTCAAACA+CGG | 0.641538 | 7.2:-8732801 | None:intergenic |
| TAATGCTGGAAAAGATGTTG+CGG | 0.662689 | 7.2:-8732543 | MS.gene051370:CDS |
| AAAGCAGAGATAAACGACCA+AGG | 0.693575 | 7.2:+8732411 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAATCTAATAATGTTACAT+AGG | + | chr7.2:8732471-8732490 | None:intergenic | 20.0% |
| !! | ATCAATATTATGGAAATAGT+TGG | - | chr7.2:8732652-8732671 | MS.gene051370:CDS | 20.0% |
| !! | ATTGTTTGATCAATACTTAT+AGG | - | chr7.2:8732486-8732505 | MS.gene051370:intron | 20.0% |
| !! | TTGTTTGATCAATACTTATA+GGG | - | chr7.2:8732487-8732506 | MS.gene051370:intron | 20.0% |
| !! | AAATCATCCATTGTTATTGT+TGG | + | chr7.2:8732365-8732384 | None:intergenic | 25.0% |
| !! | AACATAGTGAGAATCATTTT+GGG | - | chr7.2:8732532-8732551 | MS.gene051370:CDS | 25.0% |
| !!! | ATACTTATTTTGATACTCCT+TGG | - | chr7.2:8732699-8732718 | MS.gene051370:CDS | 25.0% |
| GAAATAGTTGGATGTTCAAT+GGG | - | chr7.2:8732664-8732683 | MS.gene051370:CDS | 30.0% | |
| GATGTTGAGTATTTATGTGA+AGG | - | chr7.2:8732509-8732528 | MS.gene051370:intron | 30.0% | |
| GCACATTTGATTAATAATGC+TGG | - | chr7.2:8732570-8732589 | MS.gene051370:CDS | 30.0% | |
| TGAAATGCCAACAATAACAA+TGG | - | chr7.2:8732355-8732374 | MS.gene051370:CDS | 30.0% | |
| ! | GAACATAGTGAGAATCATTT+TGG | - | chr7.2:8732531-8732550 | MS.gene051370:CDS | 30.0% |
| !! | TTGGATATGTGTTATTTGTC+TGG | - | chr7.2:8732612-8732631 | MS.gene051370:intron | 30.0% |
| AAAAGATGTTGCGGTTGATT+TGG | - | chr7.2:8732593-8732612 | MS.gene051370:intron | 35.0% | |
| GACGAGGTTCATCAATATTA+TGG | - | chr7.2:8732642-8732661 | MS.gene051370:CDS | 35.0% | |
| GGAAATAGTTGGATGTTCAA+TGG | - | chr7.2:8732663-8732682 | MS.gene051370:CDS | 35.0% | |
| TAATGCTGGAAAAGATGTTG+CGG | - | chr7.2:8732584-8732603 | MS.gene051370:CDS | 35.0% | |
| ! | GCTTTTGAACAATGTTACAG+TGG | - | chr7.2:8732413-8732432 | MS.gene051370:CDS | 35.0% |
| AAAGCAGAGATAAACGACCA+AGG | + | chr7.2:8732719-8732738 | None:intergenic | 40.0% | |
| TTGGTCGTTTATCTCTGCTT+TGG | - | chr7.2:8732718-8732737 | MS.gene051370:CDS | 40.0% | |
| !! | ATTGGTCTTGCTGATTCTCA+CGG | - | chr7.2:8732745-8732764 | MS.gene051370:CDS | 40.0% |
| !!! | GAGAATCATTTTGGGACAGA+AGG | - | chr7.2:8732540-8732559 | MS.gene051370:CDS | 40.0% |
| !!! | TTTTGGGACAGAAGGTGAAA+TGG | - | chr7.2:8732548-8732567 | MS.gene051370:CDS | 40.0% |
| TATCTCTGCTTTGGCTGCAT+TGG | - | chr7.2:8732727-8732746 | MS.gene051370:CDS | 45.0% | |
| TTTGTCTGGTGTGTTTGACG+AGG | - | chr7.2:8732626-8732645 | MS.gene051370:intron | 45.0% | |
| !! | GTCTTGCTGATTCTCACGGT+TGG | - | chr7.2:8732749-8732768 | MS.gene051370:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 8732338 | 8732811 | 8732338 | ID=MS.gene051370 |
| chr7.2 | mRNA | 8732338 | 8732811 | 8732338 | ID=MS.gene051370.t1;Parent=MS.gene051370 |
| chr7.2 | exon | 8732638 | 8732811 | 8732638 | ID=MS.gene051370.t1.exon1;Parent=MS.gene051370.t1 |
| chr7.2 | CDS | 8732638 | 8732811 | 8732638 | ID=cds.MS.gene051370.t1;Parent=MS.gene051370.t1 |
| chr7.2 | exon | 8732514 | 8732606 | 8732514 | ID=MS.gene051370.t1.exon2;Parent=MS.gene051370.t1 |
| chr7.2 | CDS | 8732514 | 8732606 | 8732514 | ID=cds.MS.gene051370.t1;Parent=MS.gene051370.t1 |
| chr7.2 | exon | 8732338 | 8732478 | 8732338 | ID=MS.gene051370.t1.exon3;Parent=MS.gene051370.t1 |
| chr7.2 | CDS | 8732338 | 8732478 | 8732338 | ID=cds.MS.gene051370.t1;Parent=MS.gene051370.t1 |
| Gene Sequence |
| Protein sequence |
