AlfalfaGEDB | MS.gene98774

M. sativa cultivar XinJiangDaYe / MS.gene98774

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98774.t1	AES65081.2	81.5	135	25	0	1	135	1	135	4.90E-47	197.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98774.t1	G7IMY6	81.5	135	25	0	1	135	1	135	3.5e-47	197.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049404	MS.gene98774	0.815974	7.02E-52	-1.69E-46
MS.gene049501	MS.gene98774	0.808439	3.10E-50	-1.69E-46
MS.gene050026	MS.gene98774	0.805634	1.21E-49	-1.69E-46
MS.gene050430	MS.gene98774	0.827449	1.56E-54	-1.69E-46
MS.gene050442	MS.gene98774	0.801573	8.45E-49	-1.69E-46
MS.gene050844	MS.gene98774	0.811801	5.84E-51	-1.69E-46
MS.gene051247	MS.gene98774	0.807775	4.29E-50	-1.69E-46
MS.gene051265	MS.gene98774	0.807079	6.02E-50	-1.69E-46
MS.gene051710	MS.gene98774	0.823897	1.08E-53	-1.69E-46
MS.gene051744	MS.gene98774	0.804139	2.49E-49	-1.69E-46
MS.gene052190	MS.gene98774	0.804941	1.70E-49	-1.69E-46
MS.gene052519	MS.gene98774	0.803921	2.77E-49	-1.69E-46
MS.gene052611	MS.gene98774	0.800188	1.62E-48	-1.69E-46
MS.gene052867	MS.gene98774	0.812741	3.64E-51	-1.69E-46
MS.gene05289	MS.gene98774	0.821506	3.89E-53	-1.69E-46
MS.gene053166	MS.gene98774	0.811533	6.68E-51	-1.69E-46
MS.gene053512	MS.gene98774	0.837091	6.38E-57	-1.69E-46
MS.gene053967	MS.gene98774	0.813531	2.44E-51	-1.69E-46
MS.gene053974	MS.gene98774	0.831857	1.32E-55	-1.69E-46
MS.gene053998	MS.gene98774	0.817575	3.07E-52	-1.69E-46
MS.gene054236	MS.gene98774	0.835028	2.13E-56	-1.69E-46
MS.gene054467	MS.gene98774	0.81771	2.86E-52	-1.69E-46
MS.gene054559	MS.gene98774	0.810006	1.43E-50	-1.69E-46
MS.gene054651	MS.gene98774	0.814279	1.67E-51	-1.69E-46
MS.gene054775	MS.gene98774	0.811796	5.85E-51	-1.69E-46
MS.gene054801	MS.gene98774	0.810321	1.22E-50	-1.69E-46
MS.gene055356	MS.gene98774	0.806633	7.48E-50	-1.69E-46
MS.gene055357	MS.gene98774	0.823944	1.05E-53	-1.69E-46
MS.gene055818	MS.gene98774	0.811942	5.44E-51	-1.69E-46
MS.gene055875	MS.gene98774	0.832631	8.47E-56	-1.69E-46
MS.gene056162	MS.gene98774	0.805868	1.08E-49	-1.69E-46
MS.gene056173	MS.gene98774	0.81121	7.85E-51	-1.69E-46
MS.gene056174	MS.gene98774	0.806422	8.29E-50	-1.69E-46
MS.gene056252	MS.gene98774	0.805465	1.32E-49	-1.69E-46
MS.gene057444	MS.gene98774	0.819829	9.45E-53	-1.69E-46
MS.gene058515	MS.gene98774	0.828596	8.23E-55	-1.69E-46
MS.gene058979	MS.gene98774	0.816153	6.40E-52	-1.69E-46
MS.gene059272	MS.gene98774	0.820257	7.55E-53	-1.69E-46
MS.gene059276	MS.gene98774	0.816808	4.57E-52	-1.69E-46
MS.gene059584	MS.gene98774	0.822626	2.14E-53	-1.69E-46
MS.gene060011	MS.gene98774	0.807118	5.91E-50	-1.69E-46
MS.gene060167	MS.gene98774	0.82643	2.73E-54	-1.69E-46
MS.gene060322	MS.gene98774	0.824306	8.67E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98774.t1	MTR_0125s0060	90.769	130	12	0	1	130	1	130	2.44e-81	235
MS.gene98774.t1	MTR_2g035110	81.481	135	25	0	1	135	1	135	7.23e-72	211
MS.gene98774.t1	MTR_8g066845	59.434	106	23	3	31	135	1	87	2.81e-28	99.8
MS.gene98774.t1	MTR_4g012560	67.742	62	15	1	48	109	24	80	1.24e-17	72.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 23 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATTGACAATGATATTTATA+TGG	0.247121	3.1:-9930076	MS.gene98774:CDS
AACAAGAAGGGGCGCATATT+TGG	0.259894	3.1:-9930036	MS.gene98774:CDS
TGAATTGAAGTGTTCATATT+AGG	0.299166	3.1:+9929811	None:intergenic
CCATTATCACCTTCTCCTTC+CGG	0.338322	3.1:+9929787	None:intergenic
TTCTACCGATGTAGATTCTT+CGG	0.370531	3.1:-9929959	MS.gene98774:CDS
AAGGGGCGCATATTTGGATT+AGG	0.373630	3.1:-9930030	MS.gene98774:CDS
GATATTTATATGGAAATTGT+TGG	0.391812	3.1:-9930066	MS.gene98774:CDS
GTTTCTCAAACGTTTGAGTT+TGG	0.397323	3.1:+9930142	None:intergenic
AAGCGCTTGGAGCAAATGAT+TGG	0.408396	3.1:-9929844	MS.gene98774:CDS
CCGGAAGGAGAAGGTGATAA+TGG	0.416904	3.1:-9929787	MS.gene98774:CDS
AGCGCTTGGAGCAAATGATT+GGG	0.510645	3.1:-9929843	MS.gene98774:CDS
TGTTGGAGGCATTAACAAGA+AGG	0.515610	3.1:-9930049	MS.gene98774:CDS
AAAACTGAAGATCAAGCGCT+TGG	0.522024	3.1:-9929857	MS.gene98774:CDS
ATTTATATGGAAATTGTTGG+AGG	0.522832	3.1:-9930063	MS.gene98774:CDS
TCATTGTCAATTTCTTCAGT+AGG	0.525205	3.1:+9930087	None:intergenic
TATGAACACTTCAATTCAGC+CGG	0.533653	3.1:-9929806	MS.gene98774:CDS
TCAATTCAGCCGGAAGGAGA+AGG	0.553755	3.1:-9929796	MS.gene98774:CDS
TCTACATCGGTAGAAATTGA+AGG	0.554924	3.1:+9929967	None:intergenic
GTTGGAGGCATTAACAAGAA+GGG	0.555037	3.1:-9930048	MS.gene98774:CDS
TTGGAGGCATTAACAAGAAG+GGG	0.580609	3.1:-9930047	MS.gene98774:CDS
TCGGTAGAAATTGAAGGCGA+TGG	0.604104	3.1:+9929973	None:intergenic
AACACTTCAATTCAGCCGGA+AGG	0.621409	3.1:-9929802	MS.gene98774:CDS
TTTGAGAAACACTCTCAAAG+TGG	0.627086	3.1:-9930129	MS.gene98774:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTGACAATGATATTTATA+TGG	-	chr3.1:9929833-9929852	MS.gene98774:CDS	15.0%
!!	GATATTTATATGGAAATTGT+TGG	-	chr3.1:9929843-9929862	MS.gene98774:CDS	20.0%
!	ATTTATATGGAAATTGTTGG+AGG	-	chr3.1:9929846-9929865	MS.gene98774:CDS	25.0%
!!	TGAATTGAAGTGTTCATATT+AGG	+	chr3.1:9930101-9930120	None:intergenic	25.0%
	TCATTGTCAATTTCTTCAGT+AGG	+	chr3.1:9929825-9929844	None:intergenic	30.0%
!	TTTTTCCGAAGAATCTACAT+CGG	+	chr3.1:9929958-9929977	None:intergenic	30.0%
	TATGAACACTTCAATTCAGC+CGG	-	chr3.1:9930103-9930122	MS.gene98774:CDS	35.0%
	TCTACATCGGTAGAAATTGA+AGG	+	chr3.1:9929945-9929964	None:intergenic	35.0%
	TTCTACCGATGTAGATTCTT+CGG	-	chr3.1:9929950-9929969	MS.gene98774:CDS	35.0%
	TTTGAGAAACACTCTCAAAG+TGG	-	chr3.1:9929780-9929799	MS.gene98774:CDS	35.0%
!	GTTTCTCAAACGTTTGAGTT+TGG	+	chr3.1:9929770-9929789	None:intergenic	35.0%
	AAAACTGAAGATCAAGCGCT+TGG	-	chr3.1:9930052-9930071	MS.gene98774:CDS	40.0%
	TTCGGAAAAAGTTGCTGCTA+TGG	-	chr3.1:9929968-9929987	MS.gene98774:CDS	40.0%
!!	GTTGGAGGCATTAACAAGAA+GGG	-	chr3.1:9929861-9929880	MS.gene98774:CDS	40.0%
!!	TGTTGGAGGCATTAACAAGA+AGG	-	chr3.1:9929860-9929879	MS.gene98774:CDS	40.0%
!!	TTGGAGGCATTAACAAGAAG+GGG	-	chr3.1:9929862-9929881	MS.gene98774:CDS	40.0%
	AACACTTCAATTCAGCCGGA+AGG	-	chr3.1:9930107-9930126	MS.gene98774:CDS	45.0%
	AAGCGCTTGGAGCAAATGAT+TGG	-	chr3.1:9930065-9930084	MS.gene98774:CDS	45.0%
	AGCGCTTGGAGCAAATGATT+GGG	-	chr3.1:9930066-9930085	MS.gene98774:CDS	45.0%
	CCATTATCACCTTCTCCTTC+CGG	+	chr3.1:9930125-9930144	None:intergenic	45.0%
	GGAAAAAGTTGCTGCTATGG+AGG	-	chr3.1:9929971-9929990	MS.gene98774:CDS	45.0%
	TCGGTAGAAATTGAAGGCGA+TGG	+	chr3.1:9929939-9929958	None:intergenic	45.0%
!	AACAAGAAGGGGCGCATATT+TGG	-	chr3.1:9929873-9929892	MS.gene98774:CDS	45.0%
!	AAGGGGCGCATATTTGGATT+AGG	-	chr3.1:9929879-9929898	MS.gene98774:CDS	45.0%
	CCGGAAGGAGAAGGTGATAA+TGG	-	chr3.1:9930122-9930141	MS.gene98774:CDS	50.0%
	TCAATTCAGCCGGAAGGAGA+AGG	-	chr3.1:9930113-9930132	MS.gene98774:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	9929762	9930169	9929762	ID=MS.gene98774
chr3.1	mRNA	9929762	9930169	9929762	ID=MS.gene98774.t1;Parent=MS.gene98774
chr3.1	exon	9929762	9930169	9929762	ID=MS.gene98774.t1.exon1;Parent=MS.gene98774.t1
chr3.1	CDS	9929762	9930169	9929762	ID=cds.MS.gene98774.t1;Parent=MS.gene98774.t1

Gene Sequence

>MS.gene98774

ATGTACCAAACTCAAACGTTTGAGAAACACTCTCAAAGTGGTGAGATTAGTAGTCAACAACCTACTGAAGAAATTGACAATGATATTTATATGGAAATTGTTGGAGGCATTAACAAGAAGGGGCGCATATTTGGATTAGGATCTCAAGTTGTTGTTGTCAAAGAATCTTTAAGACCATCGCCTTCAATTTCTACCGATGTAGATTCTTCGGAAAAAGTTGCTGCTATGGAGGCTAAGATTGAAGCATTAACTTCTGAACTTGAAAAGAAAAATCTTGAACAAGAAACGCTAAAACTGAAGATCAAGCGCTTGGAGCAAATGATTGGGAATATTGTGCCTAATATGAACACTTCAATTCAGCCGGAAGGAGAAGGTGATAATGGTAACAACGAGATAGATGAGCTTTAG

Protein sequence

>MS.gene98774.t1

MYQTQTFEKHSQSGEISSQQPTEEIDNDIYMEIVGGINKKGRIFGLGSQVVVVKESLRPSPSISTDVDSSEKVAAMEAKIEALTSELEKKNLEQETLKLKIKRLEQMIGNIVPNMNTSIQPEGEGDNGNNEIDEL