Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048073.01.T01 | KAG5398276.1 | 52.697 | 241 | 73 | 8 | 1 | 203 | 64 | 301 | 1.86E-64 | 211 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048073.01.T01 | Q69TW5 | 43.662 | 284 | 78 | 7 | 1 | 202 | 40 | 323 | 1.90E-48 | 163 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048073.01.T01 | G7JL68 | 97.087 | 103 | 3 | 0 | 1 | 103 | 48 | 150 | 1.32e-63 | 211 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048073.01 | MsG0180003189.01 | 0.806107 | 9.656279e-50 | 5.197208e-47 |
| MsG0080048073.01 | MsG0180005896.01 | 0.832841 | 7.508543e-56 | 8.493565e-53 |
| MsG0080048073.01 | MsG0280007023.01 | 0.824427 | 8.120116e-54 | 7.189800e-51 |
| MsG0080048073.01 | MsG0280008114.01 | 0.820349 | 7.190866e-53 | 5.675687e-50 |
| MsG0080048073.01 | MsG0280010945.01 | 0.823122 | 1.641775e-53 | 1.401127e-50 |
| MsG0080048073.01 | MsG0380011895.01 | 0.824875 | 6.367874e-54 | 5.712233e-51 |
| MsG0080048073.01 | MsG0380014277.01 | 0.854282 | 1.365200e-61 | 3.033624e-58 |
| MsG0080048073.01 | MsG0380016285.01 | 0.812518 | 4.071209e-51 | 2.594100e-48 |
| MsG0080048073.01 | MsG0380017326.01 | 0.817060 | 4.009949e-52 | 2.890309e-49 |
| MsG0080048073.01 | MsG0380017543.01 | 0.821628 | 3.649154e-53 | 2.984541e-50 |
| MsG0080048073.01 | MsG0480018214.01 | 0.837320 | 5.573332e-57 | 7.212825e-54 |
| MsG0080048073.01 | MsG0480018236.01 | 0.811853 | 5.687597e-51 | 3.559431e-48 |
| MsG0080048073.01 | MsG0480019434.01 | 0.808109 | 3.639565e-50 | 2.063023e-47 |
| MsG0080048073.01 | MsG0480020322.01 | 0.822704 | 2.054336e-53 | 1.732580e-50 |
| MsG0080048073.01 | MsG0480020323.01 | 0.814197 | 1.741313e-51 | 1.160771e-48 |
| MsG0080048073.01 | MsG0480021280.01 | 0.800168 | 1.635853e-48 | 7.564676e-46 |
| MsG0080048073.01 | MsG0480021558.01 | 0.804633 | 1.967257e-49 | 1.019395e-46 |
| MsG0080048073.01 | MsG0480021959.01 | 0.815927 | 7.194842e-52 | 5.027130e-49 |
| MsG0080048073.01 | MsG0580024160.01 | 0.815597 | 8.522762e-52 | 5.901829e-49 |
| MsG0080048073.01 | MsG0580024495.01 | 0.812492 | 4.125766e-51 | 2.627050e-48 |
| MsG0080048073.01 | MsG0580024935.01 | 0.817736 | 2.824383e-52 | 2.073821e-49 |
| MsG0080048073.01 | MsG0580025467.01 | 0.801557 | 8.513129e-49 | 4.077711e-46 |
| MsG0080048073.01 | MsG0580025832.01 | 0.827195 | 1.788887e-54 | 1.714899e-51 |
| MsG0080048073.01 | MsG0580026304.01 | 0.823859 | 1.103795e-53 | 9.618474e-51 |
| MsG0080048073.01 | MsG0580029038.01 | 0.812450 | 4.212960e-51 | 2.679739e-48 |
| MsG0080048073.01 | MsG0580029924.01 | 0.814613 | 1.409172e-51 | 9.500290e-49 |
| MsG0080048073.01 | MsG0680030415.01 | 0.810913 | 9.100554e-51 | 5.555941e-48 |
| MsG0080048073.01 | MsG0680031334.01 | 0.856400 | 3.301294e-62 | 7.869804e-59 |
| MsG0080048073.01 | MsG0680031692.01 | 0.820933 | 5.278812e-53 | 4.234837e-50 |
| MsG0080048073.01 | MsG0680032058.01 | 0.812496 | 4.117928e-51 | 2.622302e-48 |
| MsG0080048073.01 | MsG0680034909.01 | -0.814743 | 1.319023e-51 | 8.923348e-49 |
| MsG0080048073.01 | MsG0780039143.01 | 0.814584 | 1.430121e-51 | 9.633973e-49 |
| MsG0080048073.01 | MsG0780039144.01 | 0.802259 | 6.107199e-49 | 2.977994e-46 |
| MsG0080048073.01 | MsG0780040177.01 | 0.847346 | 1.226049e-59 | 2.172575e-56 |
| MsG0080048073.01 | MsG0780040374.01 | 0.801726 | 7.858446e-49 | 3.780204e-46 |
| MsG0080048073.01 | MsG0780040518.01 | 0.817616 | 3.007418e-52 | 2.200769e-49 |
| MsG0080048073.01 | MsG0780040576.01 | -0.803964 | 2.710791e-49 | 1.380814e-46 |
| MsG0080048073.01 | MsG0780040596.01 | 0.803608 | 3.213544e-49 | 1.621999e-46 |
| MsG0080048073.01 | MsG0880043905.01 | 0.832291 | 1.027868e-55 | 1.143720e-52 |
| MsG0080048073.01 | MsG0880045285.01 | 0.813331 | 2.702169e-51 | 1.759486e-48 |
| MsG0080048073.01 | MsG0880045555.01 | -0.809749 | 1.622708e-50 | 9.606878e-48 |
| MsG0080048073.01 | MsG0880045783.01 | 0.820978 | 5.153475e-53 | 4.139798e-50 |
| MsG0080048073.01 | MsG0880046569.01 | -0.807234 | 5.584327e-50 | 3.093907e-47 |
| MsG0080048073.01 | MsG0880047045.01 | 0.804055 | 2.595306e-49 | 1.325127e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048073.01.T01 | MTR_4g085910 | 97.087 | 103 | 3 | 0 | 1 | 103 | 48 | 150 | 3.34e-67 | 211 |
| MsG0080048073.01.T01 | MTR_4g085910 | 93.137 | 102 | 7 | 0 | 102 | 203 | 275 | 376 | 1.28e-60 | 194 |
| MsG0080048073.01.T01 | MTR_8g043960 | 40.164 | 244 | 74 | 9 | 1 | 177 | 54 | 292 | 4.45e-36 | 128 |
| MsG0080048073.01.T01 | MTR_3g101780 | 68.627 | 102 | 24 | 3 | 110 | 203 | 330 | 431 | 1.37e-31 | 119 |
| MsG0080048073.01.T01 | MTR_3g101780 | 60.000 | 105 | 26 | 4 | 1 | 99 | 57 | 151 | 1.66e-28 | 110 |
| MsG0080048073.01.T01 | MTR_3g010660 | 34.615 | 260 | 81 | 9 | 1 | 179 | 50 | 301 | 1.38e-26 | 103 |
| MsG0080048073.01.T01 | MTR_2g086390 | 32.751 | 229 | 94 | 7 | 1 | 175 | 35 | 257 | 4.62e-22 | 91.3 |
| MsG0080048073.01.T01 | MTR_2g086390 | 31.111 | 225 | 95 | 7 | 1 | 171 | 35 | 253 | 1.54e-21 | 89.7 |
| MsG0080048073.01.T01 | MTR_4g094390 | 38.194 | 144 | 65 | 5 | 54 | 175 | 36 | 177 | 5.47e-19 | 81.3 |
| MsG0080048073.01.T01 | MTR_5g088230 | 37.745 | 204 | 89 | 9 | 1 | 176 | 52 | 245 | 2.50e-18 | 80.9 |
| MsG0080048073.01.T01 | MTR_5g088230 | 37.745 | 204 | 89 | 9 | 1 | 176 | 47 | 240 | 2.58e-18 | 80.9 |
| MsG0080048073.01.T01 | MTR_7g088090 | 53.261 | 92 | 35 | 2 | 115 | 202 | 232 | 319 | 9.34e-17 | 77.4 |
| MsG0080048073.01.T01 | MTR_7g088090 | 42.424 | 99 | 52 | 2 | 1 | 97 | 46 | 141 | 3.59e-15 | 72.8 |
| MsG0080048073.01.T01 | MTR_6g026800 | 42.718 | 103 | 47 | 4 | 1 | 93 | 46 | 146 | 4.91e-13 | 67.0 |
| MsG0080048073.01.T01 | MTR_1g098590 | 45.679 | 81 | 41 | 1 | 123 | 203 | 157 | 234 | 9.99e-13 | 65.1 |
| MsG0080048073.01.T01 | MTR_1g098590 | 60.377 | 53 | 21 | 0 | 123 | 175 | 157 | 209 | 1.25e-12 | 64.7 |
| MsG0080048073.01.T01 | MTR_1g098590 | 60.377 | 53 | 21 | 0 | 123 | 175 | 157 | 209 | 1.81e-12 | 64.3 |
| MsG0080048073.01.T01 | MTR_7g015990 | 68.627 | 51 | 13 | 1 | 128 | 175 | 120 | 170 | 3.77e-12 | 62.8 |
| MsG0080048073.01.T01 | MTR_5g022780 | 51.220 | 82 | 23 | 2 | 128 | 192 | 166 | 247 | 1.35e-11 | 62.0 |
| MsG0080048073.01.T01 | MTR_7g104480 | 51.282 | 78 | 27 | 2 | 134 | 200 | 351 | 428 | 3.95e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048073.01.T01 | AT3G56850 | 39.773 | 264 | 86 | 10 | 1 | 203 | 46 | 297 | 1.33e-38 | 135 |
| MsG0080048073.01.T01 | AT2G41070 | 45.263 | 190 | 61 | 6 | 32 | 180 | 56 | 243 | 3.66e-36 | 127 |
| MsG0080048073.01.T01 | AT2G41070 | 46.961 | 181 | 53 | 6 | 32 | 171 | 56 | 234 | 2.13e-35 | 125 |
| MsG0080048073.01.T01 | AT2G41070 | 46.961 | 181 | 53 | 6 | 32 | 171 | 56 | 234 | 2.13e-35 | 125 |
| MsG0080048073.01.T01 | AT2G41070 | 46.961 | 181 | 53 | 6 | 32 | 171 | 56 | 234 | 2.13e-35 | 125 |
| MsG0080048073.01.T01 | AT2G41070 | 46.961 | 181 | 53 | 6 | 32 | 171 | 65 | 243 | 2.29e-35 | 125 |
| MsG0080048073.01.T01 | AT1G45249 | 70.000 | 100 | 23 | 2 | 111 | 203 | 317 | 416 | 3.11e-34 | 125 |
| MsG0080048073.01.T01 | AT1G45249 | 53.913 | 115 | 26 | 3 | 1 | 95 | 48 | 155 | 3.33e-23 | 96.3 |
| MsG0080048073.01.T01 | AT1G45249 | 70.000 | 100 | 23 | 2 | 111 | 203 | 332 | 431 | 4.88e-34 | 125 |
| MsG0080048073.01.T01 | AT1G45249 | 53.913 | 115 | 26 | 3 | 1 | 95 | 63 | 170 | 3.63e-23 | 96.3 |
| MsG0080048073.01.T01 | AT1G45249 | 70.000 | 100 | 23 | 2 | 111 | 203 | 332 | 431 | 4.88e-34 | 125 |
| MsG0080048073.01.T01 | AT1G45249 | 53.913 | 115 | 26 | 3 | 1 | 95 | 63 | 170 | 3.63e-23 | 96.3 |
| MsG0080048073.01.T01 | AT3G19290 | 56.557 | 122 | 45 | 3 | 89 | 203 | 312 | 432 | 3.24e-32 | 120 |
| MsG0080048073.01.T01 | AT3G19290 | 49.580 | 119 | 41 | 3 | 1 | 102 | 64 | 180 | 2.01e-21 | 91.3 |
| MsG0080048073.01.T01 | AT3G19290 | 57.500 | 120 | 46 | 3 | 89 | 203 | 312 | 431 | 4.73e-32 | 120 |
| MsG0080048073.01.T01 | AT3G19290 | 49.580 | 119 | 41 | 3 | 1 | 102 | 64 | 180 | 1.94e-21 | 91.3 |
| MsG0080048073.01.T01 | AT3G19290 | 57.500 | 120 | 46 | 3 | 89 | 203 | 312 | 431 | 4.73e-32 | 120 |
| MsG0080048073.01.T01 | AT3G19290 | 49.580 | 119 | 41 | 3 | 1 | 102 | 64 | 180 | 1.94e-21 | 91.3 |
| MsG0080048073.01.T01 | AT1G45249 | 63.063 | 111 | 23 | 3 | 111 | 203 | 317 | 427 | 6.78e-31 | 117 |
| MsG0080048073.01.T01 | AT1G45249 | 53.913 | 115 | 26 | 3 | 1 | 95 | 48 | 155 | 4.27e-23 | 95.9 |
| MsG0080048073.01.T01 | AT4G34000 | 61.682 | 107 | 34 | 2 | 104 | 203 | 348 | 454 | 3.76e-30 | 115 |
| MsG0080048073.01.T01 | AT4G34000 | 48.855 | 131 | 30 | 5 | 1 | 94 | 58 | 188 | 1.96e-25 | 102 |
| MsG0080048073.01.T01 | AT4G34000 | 61.682 | 107 | 34 | 2 | 104 | 203 | 348 | 454 | 3.76e-30 | 115 |
| MsG0080048073.01.T01 | AT4G34000 | 48.855 | 131 | 30 | 5 | 1 | 94 | 58 | 188 | 1.96e-25 | 102 |
| MsG0080048073.01.T01 | AT4G34000 | 61.682 | 107 | 34 | 2 | 104 | 203 | 348 | 454 | 3.76e-30 | 115 |
| MsG0080048073.01.T01 | AT4G34000 | 48.855 | 131 | 30 | 5 | 1 | 94 | 58 | 188 | 1.96e-25 | 102 |
| MsG0080048073.01.T01 | AT1G49720 | 52.459 | 122 | 52 | 2 | 88 | 203 | 271 | 392 | 3.42e-29 | 112 |
| MsG0080048073.01.T01 | AT1G49720 | 56.863 | 102 | 36 | 2 | 1 | 94 | 53 | 154 | 2.46e-28 | 109 |
| MsG0080048073.01.T01 | AT1G49720 | 52.459 | 122 | 52 | 2 | 88 | 203 | 271 | 392 | 3.42e-29 | 112 |
| MsG0080048073.01.T01 | AT1G49720 | 56.863 | 102 | 36 | 2 | 1 | 94 | 53 | 154 | 2.46e-28 | 109 |
| MsG0080048073.01.T01 | AT1G49720 | 56.863 | 102 | 36 | 2 | 1 | 94 | 53 | 154 | 1.43e-28 | 110 |
| MsG0080048073.01.T01 | AT1G49720 | 55.670 | 97 | 42 | 1 | 88 | 183 | 271 | 367 | 3.32e-25 | 101 |
| MsG0080048073.01.T01 | AT3G19290 | 70.667 | 75 | 21 | 1 | 110 | 183 | 333 | 407 | 4.58e-27 | 106 |
| MsG0080048073.01.T01 | AT3G19290 | 49.580 | 119 | 41 | 3 | 1 | 102 | 64 | 180 | 1.33e-21 | 91.7 |
| MsG0080048073.01.T01 | AT4G34000 | 48.855 | 131 | 30 | 5 | 1 | 94 | 58 | 188 | 5.70e-26 | 103 |
| MsG0080048073.01.T01 | AT4G34000 | 71.831 | 71 | 19 | 1 | 104 | 173 | 348 | 418 | 2.84e-23 | 96.7 |
| MsG0080048073.01.T01 | AT1G45249 | 53.913 | 115 | 26 | 3 | 1 | 95 | 48 | 155 | 2.67e-23 | 96.3 |
| MsG0080048073.01.T01 | AT1G45249 | 53.913 | 115 | 26 | 3 | 1 | 95 | 48 | 155 | 2.67e-23 | 96.3 |
| MsG0080048073.01.T01 | AT1G45249 | 53.913 | 115 | 26 | 3 | 1 | 95 | 48 | 155 | 2.67e-23 | 96.3 |
| MsG0080048073.01.T01 | AT1G45249 | 53.913 | 115 | 26 | 3 | 1 | 95 | 48 | 155 | 2.67e-23 | 96.3 |
| MsG0080048073.01.T01 | AT1G45249 | 51.304 | 115 | 29 | 3 | 1 | 95 | 48 | 155 | 3.06e-23 | 95.9 |
| MsG0080048073.01.T01 | AT1G45249 | 70.213 | 47 | 11 | 1 | 111 | 154 | 317 | 363 | 8.39e-12 | 63.5 |
| MsG0080048073.01.T01 | AT1G45249 | 51.304 | 115 | 29 | 3 | 1 | 95 | 48 | 155 | 3.06e-23 | 95.9 |
| MsG0080048073.01.T01 | AT1G45249 | 70.213 | 47 | 11 | 1 | 111 | 154 | 317 | 363 | 8.39e-12 | 63.5 |
| MsG0080048073.01.T01 | AT3G19290 | 49.580 | 119 | 41 | 3 | 1 | 102 | 64 | 180 | 1.84e-21 | 91.3 |
| MsG0080048073.01.T01 | AT3G19290 | 51.562 | 64 | 30 | 1 | 89 | 151 | 312 | 375 | 8.03e-11 | 60.8 |
| MsG0080048073.01.T01 | AT2G36270 | 73.438 | 64 | 14 | 1 | 116 | 176 | 341 | 404 | 1.80e-18 | 83.2 |
| MsG0080048073.01.T01 | AT2G36270 | 73.438 | 64 | 14 | 1 | 116 | 176 | 341 | 404 | 1.80e-18 | 83.2 |
| MsG0080048073.01.T01 | AT2G36270 | 73.438 | 64 | 14 | 1 | 116 | 176 | 341 | 404 | 2.14e-18 | 82.8 |
| MsG0080048073.01.T01 | AT5G44080 | 36.478 | 159 | 61 | 5 | 52 | 172 | 120 | 276 | 1.62e-16 | 76.6 |
| MsG0080048073.01.T01 | AT4G35900 | 53.333 | 75 | 30 | 2 | 129 | 203 | 216 | 285 | 6.77e-14 | 68.9 |
| MsG0080048073.01.T01 | AT3G44460 | 32.971 | 276 | 106 | 11 | 1 | 203 | 62 | 331 | 1.53e-13 | 68.6 |
| MsG0080048073.01.T01 | AT2G17770 | 51.316 | 76 | 32 | 2 | 128 | 203 | 125 | 195 | 4.36e-13 | 65.5 |
| MsG0080048073.01.T01 | AT1G03970 | 33.103 | 145 | 70 | 3 | 52 | 169 | 85 | 229 | 4.82e-13 | 66.6 |
| MsG0080048073.01.T01 | AT5G42910 | 40.404 | 99 | 31 | 3 | 1 | 90 | 47 | 126 | 4.23e-11 | 61.6 |
Find 62 sgRNAs with CRISPR-Local
Find 116 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGATACAGGCAGAGTTTA+TGG | 0.190438 | contig192end:-4741 | MsG0080048073.01.T01:intron |
| CTAAACCATTCATTATTATT+TGG | 0.243582 | contig192end:+5651 | None:intergenic |
| AGAGAAAAGTTGATGAAGTT+TGG | 0.248226 | contig192end:-5811 | MsG0080048073.01.T01:CDS |
| CTACCACCATTTGGGTTGTT+AGG | 0.265606 | contig192end:+5878 | None:intergenic |
| CCTAATGTTGGTTGTCTTTG+AGG | 0.269893 | contig192end:+5755 | None:intergenic |
| TACTTGAGAAGGACACTTAC+AGG | 0.325650 | contig192end:-4671 | MsG0080048073.01.T01:CDS |
| AGCTAATATGTATAGGTATT+AGG | 0.365572 | contig192end:-4534 | MsG0080048073.01.T01:three_prime_UTR |
| CTCCCTCTGTTGATTGCATA+AGG | 0.369742 | contig192end:+5256 | None:intergenic |
| TTTGCAAACTACCACCATTT+GGG | 0.380553 | contig192end:+5870 | None:intergenic |
| AAGTGTGTTCCTGTAGAAAA+AGG | 0.390691 | contig192end:-5232 | MsG0080048073.01.T01:CDS |
| GAGGTCACGGGCTCGCAAAC+AGG | 0.396241 | contig192end:-5161 | MsG0080048073.01.T01:intron |
| CTCCTGTCCTTGTGACATAA+TGG | 0.398467 | contig192end:+4605 | None:intergenic |
| TTCAATTGTTGAGCATGATT+AGG | 0.401510 | contig192end:+5677 | None:intergenic |
| AGAAACATAGACAGTCTAAC+AGG | 0.414524 | contig192end:+4380 | None:intergenic |
| AACATTAGGTGAAGTGACTT+TGG | 0.430676 | contig192end:-5741 | MsG0080048073.01.T01:CDS |
| CCTCAAAGACAACCAACATT+AGG | 0.434960 | contig192end:-5755 | MsG0080048073.01.T01:CDS |
| TTTCTCTGACTAAGTGTCCT+TGG | 0.439082 | contig192end:+5827 | None:intergenic |
| GTCTATGTTTCTCACCTCTC+TGG | 0.440791 | contig192end:-4369 | MsG0080048073.01.T01:three_prime_UTR |
| GGCTGAAGTTGCAAAACTTA+AGG | 0.442265 | contig192end:-4879 | MsG0080048073.01.T01:CDS |
| TATGGAAATGCAGAAAAGTA+AGG | 0.444656 | contig192end:-4723 | MsG0080048073.01.T01:CDS |
| CGAGGAATTACAAAGAAAAC+AGG | 0.444987 | contig192end:-4849 | MsG0080048073.01.T01:intron |
| CAAGCATTGACATCTCTAAC+TGG | 0.446103 | contig192end:-5920 | MsG0080048073.01.T01:CDS |
| GCAGAAAAGTAAGGAGGATC+TGG | 0.454613 | contig192end:-4714 | MsG0080048073.01.T01:CDS |
| AAAGGCCTACACTGTTGAAC+TGG | 0.460693 | contig192end:-4903 | MsG0080048073.01.T01:intron |
| AAAGACCAAATAATAATGAA+TGG | 0.465002 | contig192end:-5656 | MsG0080048073.01.T01:intron |
| GGACAGGAGTATTGTATGTA+AGG | 0.469582 | contig192end:-4591 | MsG0080048073.01.T01:three_prime_UTR |
| AAAAGTTGATGAAGTTTGGA+GGG | 0.481612 | contig192end:-5807 | MsG0080048073.01.T01:CDS |
| GGATAGTAGCTAATATGTAT+AGG | 0.482563 | contig192end:-4541 | MsG0080048073.01.T01:three_prime_UTR |
| AGGGATTTGATGAAAGATAG+TGG | 0.487452 | contig192end:-5788 | MsG0080048073.01.T01:CDS |
| GAGAATCAGCTGCGAGGTCA+CGG | 0.489882 | contig192end:-5174 | MsG0080048073.01.T01:CDS |
| GAAAAGTTGATGAAGTTTGG+AGG | 0.496145 | contig192end:-5808 | MsG0080048073.01.T01:CDS |
| TCTCTAACTGGTGGAGGAGT+AGG | 0.517394 | contig192end:-5908 | MsG0080048073.01.T01:CDS |
| AAATAGTTAATTACACAAGA+TGG | 0.517869 | contig192end:+4473 | None:intergenic |
| TAACAGGGGCACAGGCAGGC+TGG | 0.531222 | contig192end:+4396 | None:intergenic |
| AGCCATTATGTCACAAGGAC+AGG | 0.531997 | contig192end:-4607 | MsG0080048073.01.T01:three_prime_UTR |
| GAAGGACCTAACAACCCAAA+TGG | 0.541425 | contig192end:-5884 | MsG0080048073.01.T01:CDS |
| AGAATCAGCTGCGAGGTCAC+GGG | 0.543539 | contig192end:-5173 | MsG0080048073.01.T01:CDS |
| ATAAAATAAAATACTTGAGA+AGG | 0.546602 | contig192end:-4682 | MsG0080048073.01.T01:CDS |
| AAAGTCACTTCACCTAATGT+TGG | 0.567590 | contig192end:+5743 | None:intergenic |
| AGTCTAACAGGGGCACAGGC+AGG | 0.568754 | contig192end:+4392 | None:intergenic |
| ATTAGGTGAAGTGACTTTGG+AGG | 0.571566 | contig192end:-5738 | MsG0080048073.01.T01:CDS |
| AGACAGTCTAACAGGGGCAC+AGG | 0.580440 | contig192end:+4388 | None:intergenic |
| TTGACATCTCTAACTGGTGG+AGG | 0.580857 | contig192end:-5914 | MsG0080048073.01.T01:CDS |
| TTCCTTATGCAATCAACAGA+GGG | 0.614058 | contig192end:-5258 | MsG0080048073.01.T01:CDS |
| GAAGGACACTTACAGGTCCA+TGG | 0.625288 | contig192end:-4664 | MsG0080048073.01.T01:CDS |
| GGCCTACACTGTTGAACTGG+AGG | 0.626943 | contig192end:-4900 | MsG0080048073.01.T01:CDS |
| GCATTGACATCTCTAACTGG+TGG | 0.627413 | contig192end:-5917 | MsG0080048073.01.T01:CDS |
| ACTGGTGGAGGAGTAGGAGA+AGG | 0.628322 | contig192end:-5902 | MsG0080048073.01.T01:CDS |
| AAGGTTCATTGACATTGCCA+AGG | 0.629803 | contig192end:-5844 | MsG0080048073.01.T01:CDS |
| ACAACTCACATAATCTACCA+TGG | 0.634836 | contig192end:+4647 | None:intergenic |
| CAAACTTTAATTGGCCAGAG+AGG | 0.650703 | contig192end:+4355 | None:intergenic |
| GAAACATAGACAGTCTAACA+GGG | 0.656176 | contig192end:+4381 | None:intergenic |
| GGAAATGCAGAAAAGTAAGG+AGG | 0.663949 | contig192end:-4720 | MsG0080048073.01.T01:CDS |
| AATGAAGCCATTATGTCACA+AGG | 0.664340 | contig192end:-4612 | MsG0080048073.01.T01:three_prime_UTR |
| CTTATGCAATCAACAGAGGG+AGG | 0.664407 | contig192end:-5255 | MsG0080048073.01.T01:CDS |
| GGACCTAACAACCCAAATGG+TGG | 0.667509 | contig192end:-5881 | MsG0080048073.01.T01:CDS |
| GTTCCTTATGCAATCAACAG+AGG | 0.671116 | contig192end:-5259 | MsG0080048073.01.T01:CDS |
| AGCCTCCAGTTCAACAGTGT+AGG | 0.672463 | contig192end:+4898 | None:intergenic |
| AAAGTAAGGAGGATCTGGTG+CGG | 0.707722 | contig192end:-4709 | MsG0080048073.01.T01:CDS |
| AGAATAGAGAATCAGCTGCG+AGG | 0.715608 | contig192end:-5180 | MsG0080048073.01.T01:CDS |
| AAAACTTAAGGAAGTAAACG+AGG | 0.734887 | contig192end:-4867 | MsG0080048073.01.T01:CDS |
| AAACATAGACAGTCTAACAG+GGG | 0.747776 | contig192end:+4382 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAAATAAAATACTTGAGA+AGG | - | contig192end:5599-5618 | MsG0080048073.01.T01:intron | 15.0% |
| !!! | AAATATTTGTTTCTTTTGAA+TGG | - | contig192end:5355-5374 | MsG0080048073.01.T01:intron | 15.0% |
| !!! | TTTTTACATATATTCATCAA+TGG | - | contig192end:5951-5970 | MsG0080048073.01.T01:CDS | 15.0% |
| !! | AAAGACCAAATAATAATGAA+TGG | - | contig192end:4625-4644 | MsG0080048073.01.T01:three_prime_UTR | 20.0% |
| !! | AAATAGTTAATTACACAAGA+TGG | + | contig192end:5811-5830 | None:intergenic | 20.0% |
| !! | ATACATTATATGTGTATTGA+AGG | + | contig192end:5258-5277 | None:intergenic | 20.0% |
| !! | CTAAACCATTCATTATTATT+TGG | + | contig192end:4633-4652 | None:intergenic | 20.0% |
| !! | GTAAAAACACAAACTTTAAT+TGG | + | contig192end:5938-5957 | None:intergenic | 20.0% |
| ! | AACAACCAAATGGAAATAAT+GGG | - | contig192end:4697-4716 | MsG0080048073.01.T01:CDS | 25.0% |
| ! | AGCTAATATGTATAGGTATT+AGG | - | contig192end:5747-5766 | MsG0080048073.01.T01:CDS | 25.0% |
| ! | ATAACAAGATCATAAGCTTA+AGG | - | contig192end:5276-5295 | MsG0080048073.01.T01:intron | 25.0% |
| ! | ATGAACTCTTGAAAAACATA+TGG | - | contig192end:4323-4342 | MsG0080048073.01.T01:three_prime_UTR | 25.0% |
| ! | GTATTAAATGATGTAAGCAT+TGG | - | contig192end:5311-5330 | MsG0080048073.01.T01:intron | 25.0% |
| !! | GTAATTACAATTTTACAGAC+AGG | - | contig192end:5719-5738 | MsG0080048073.01.T01:CDS | 25.0% |
| !! | TGATTCAAGTTAATTGATGA+AGG | + | contig192end:4771-4790 | None:intergenic | 25.0% |
| !!! | TTTGTTTCTTTTGAATGGAA+AGG | - | contig192end:5360-5379 | MsG0080048073.01.T01:intron | 25.0% |
| AAAACTTAAGGAAGTAAACG+AGG | - | contig192end:5414-5433 | MsG0080048073.01.T01:intron | 30.0% | |
| AACAATAACACCAATCTACT+TGG | - | contig192end:4669-4688 | MsG0080048073.01.T01:CDS | 30.0% | |
| ACAACCAAATGGAAATAATG+GGG | - | contig192end:4698-4717 | MsG0080048073.01.T01:CDS | 30.0% | |
| ATATCCCCATTATTTCCATT+TGG | + | contig192end:4705-4724 | None:intergenic | 30.0% | |
| CAACAACCAAATGGAAATAA+TGG | - | contig192end:4696-4715 | MsG0080048073.01.T01:CDS | 30.0% | |
| CATCAGTAATATGTTCACTA+AGG | - | contig192end:5486-5505 | MsG0080048073.01.T01:intron | 30.0% | |
| GCATTGTTTAGCAAATGAAT+AGG | + | contig192end:4849-4868 | None:intergenic | 30.0% | |
| GGATAGTAGCTAATATGTAT+AGG | - | contig192end:5740-5759 | MsG0080048073.01.T01:CDS | 30.0% | |
| TATGGAAATGCAGAAAAGTA+AGG | - | contig192end:5558-5577 | MsG0080048073.01.T01:intron | 30.0% | |
| TTACAGTTTCTGACTACATT+TGG | - | contig192end:5517-5536 | MsG0080048073.01.T01:intron | 30.0% | |
| TTACGCTTTGTAATCAAATC+CGG | + | contig192end:4978-4997 | None:intergenic | 30.0% | |
| TTCAATTGTTGAGCATGATT+AGG | + | contig192end:4607-4626 | None:intergenic | 30.0% | |
| ! | TTAACTTGAATCACTCTCAA+CGG | - | contig192end:4778-4797 | MsG0080048073.01.T01:intron | 30.0% |
| ! | TTCAAGTTAATTGATGAAGG+AGG | + | contig192end:4768-4787 | None:intergenic | 30.0% |
| !! | AAAAGTTGATGAAGTTTGGA+GGG | - | contig192end:4474-4493 | MsG0080048073.01.T01:three_prime_UTR | 30.0% |
| !! | AGAGAAAAGTTGATGAAGTT+TGG | - | contig192end:4470-4489 | MsG0080048073.01.T01:three_prime_UTR | 30.0% |
| !!! | TTTTTCAAGAGTTCATCCAT+GGG | + | contig192end:4319-4338 | None:intergenic | 30.0% |
| AAACATAGACAGTCTAACAG+GGG | + | contig192end:5902-5921 | None:intergenic | 35.0% | |
| AAAGTCACTTCACCTAATGT+TGG | + | contig192end:4541-4560 | None:intergenic | 35.0% | |
| AACATTAGGTGAAGTGACTT+TGG | - | contig192end:4540-4559 | MsG0080048073.01.T01:three_prime_UTR | 35.0% | |
| AAGTGTGTTCCTGTAGAAAA+AGG | - | contig192end:5049-5068 | MsG0080048073.01.T01:intron | 35.0% | |
| AATGAAGCCATTATGTCACA+AGG | - | contig192end:5669-5688 | MsG0080048073.01.T01:CDS | 35.0% | |
| AATGCTACAGTTGTAGGTTT+CGG | + | contig192end:4819-4838 | None:intergenic | 35.0% | |
| ACAACTCACATAATCTACCA+TGG | + | contig192end:5637-5656 | None:intergenic | 35.0% | |
| AGAAACATAGACAGTCTAAC+AGG | + | contig192end:5904-5923 | None:intergenic | 35.0% | |
| AGGGATTTGATGAAAGATAG+TGG | - | contig192end:4493-4512 | MsG0080048073.01.T01:three_prime_UTR | 35.0% | |
| CGAGGAATTACAAAGAAAAC+AGG | - | contig192end:5432-5451 | MsG0080048073.01.T01:intron | 35.0% | |
| GAAACATAGACAGTCTAACA+GGG | + | contig192end:5903-5922 | None:intergenic | 35.0% | |
| GAAGAAAATGCTACAGTTGT+AGG | + | contig192end:4825-4844 | None:intergenic | 35.0% | |
| GACTACATTTGGTATGATAC+AGG | - | contig192end:5528-5547 | MsG0080048073.01.T01:intron | 35.0% | |
| GATCAGAGAAACAAAACAGA+TGG | + | contig192end:5199-5218 | None:intergenic | 35.0% | |
| TATGATACAGGCAGAGTTTA+TGG | - | contig192end:5540-5559 | MsG0080048073.01.T01:intron | 35.0% | |
| TTAATTGATGAAGGAGGAAG+AGG | + | contig192end:4762-4781 | None:intergenic | 35.0% | |
| TTCCTTATGCAATCAACAGA+GGG | - | contig192end:5023-5042 | MsG0080048073.01.T01:intron | 35.0% | |
| TTGCTAAACAATGCTCAACT+TGG | - | contig192end:4855-4874 | MsG0080048073.01.T01:CDS | 35.0% | |
| TTGTTGAAAGCCAAGTAGAT+TGG | + | contig192end:4682-4701 | None:intergenic | 35.0% | |
| TTTGCAAACTACCACCATTT+GGG | + | contig192end:4414-4433 | None:intergenic | 35.0% | |
| ! | AATGCTCAACTTGGTAACAA+TGG | - | contig192end:4864-4883 | MsG0080048073.01.T01:CDS | 35.0% |
| ! | CTTTCAACACCTTTTTCTAC+AGG | + | contig192end:5061-5080 | None:intergenic | 35.0% |
| ! | TTTTGCAAACTACCACCATT+TGG | + | contig192end:4415-4434 | None:intergenic | 35.0% |
| !! | CTGTAGAAAAAGGTGTTGAA+AGG | - | contig192end:5059-5078 | MsG0080048073.01.T01:intron | 35.0% |
| !! | GAAAAGTTGATGAAGTTTGG+AGG | - | contig192end:4473-4492 | MsG0080048073.01.T01:three_prime_UTR | 35.0% |
| !!! | GTTTTTCAAGAGTTCATCCA+TGG | + | contig192end:4320-4339 | None:intergenic | 35.0% |
| AAGGTTCATTGACATTGCCA+AGG | - | contig192end:4437-4456 | MsG0080048073.01.T01:three_prime_UTR | 40.0% | |
| ATGCTACAGTTGTAGGTTTC+GGG | + | contig192end:4818-4837 | None:intergenic | 40.0% | |
| ATTAGGTGAAGTGACTTTGG+AGG | - | contig192end:4543-4562 | MsG0080048073.01.T01:three_prime_UTR | 40.0% | |
| CAAACTTTAATTGGCCAGAG+AGG | + | contig192end:5929-5948 | None:intergenic | 40.0% | |
| CCTCAAAGACAACCAACATT+AGG | - | contig192end:4526-4545 | MsG0080048073.01.T01:three_prime_UTR | 40.0% | |
| GGAAATGCAGAAAAGTAAGG+AGG | - | contig192end:5561-5580 | MsG0080048073.01.T01:intron | 40.0% | |
| GGCTGAAGTTGCAAAACTTA+AGG | - | contig192end:5402-5421 | MsG0080048073.01.T01:intron | 40.0% | |
| GTTCCTTATGCAATCAACAG+AGG | - | contig192end:5022-5041 | MsG0080048073.01.T01:intron | 40.0% | |
| TACTTGAGAAGGACACTTAC+AGG | - | contig192end:5610-5629 | MsG0080048073.01.T01:intron | 40.0% | |
| TTACTTCCTGGAGAAGTAAC+AGG | + | contig192end:4948-4967 | None:intergenic | 40.0% | |
| TTTCTCTGACTAAGTGTCCT+TGG | + | contig192end:4457-4476 | None:intergenic | 40.0% | |
| ! | CCTAATGTTGGTTGTCTTTG+AGG | + | contig192end:4529-4548 | None:intergenic | 40.0% |
| ! | CTGTTGATTGCATAAGGAAC+TGG | + | contig192end:5022-5041 | None:intergenic | 40.0% |
| ! | CTTGGCTTTCAACAACCAAA+TGG | - | contig192end:4687-4706 | MsG0080048073.01.T01:CDS | 40.0% |
| ! | GGTGGTAGTTTGCAAAAACA+AGG | - | contig192end:4418-4437 | MsG0080048073.01.T01:three_prime_UTR | 40.0% |
| !! | AGTGACTTTGGAGGAGTTTT+TGG | - | contig192end:4552-4571 | MsG0080048073.01.T01:three_prime_UTR | 40.0% |
| !! | CAAGCATTGACATCTCTAAC+TGG | - | contig192end:4361-4380 | MsG0080048073.01.T01:three_prime_UTR | 40.0% |
| !! | GGACAGGAGTATTGTATGTA+AGG | - | contig192end:5690-5709 | MsG0080048073.01.T01:CDS | 40.0% |
| AAACCATGCATTCCAACACC+AGG | + | contig192end:4903-4922 | None:intergenic | 45.0% | |
| AAAGGCCTACACTGTTGAAC+TGG | - | contig192end:5378-5397 | MsG0080048073.01.T01:intron | 45.0% | |
| AAAGTAAGGAGGATCTGGTG+CGG | - | contig192end:5572-5591 | MsG0080048073.01.T01:intron | 45.0% | |
| AACAGGAGCTGTGACATTTG+AGG | + | contig192end:4931-4950 | None:intergenic | 45.0% | |
| AGAATAGAGAATCAGCTGCG+AGG | - | contig192end:5101-5120 | MsG0080048073.01.T01:intron | 45.0% | |
| AGCCATTATGTCACAAGGAC+AGG | - | contig192end:5674-5693 | MsG0080048073.01.T01:CDS | 45.0% | |
| CTCCTGTCCTTGTGACATAA+TGG | + | contig192end:5679-5698 | None:intergenic | 45.0% | |
| CTTATGCAATCAACAGAGGG+AGG | - | contig192end:5026-5045 | MsG0080048073.01.T01:intron | 45.0% | |
| GAAGGACCTAACAACCCAAA+TGG | - | contig192end:4397-4416 | MsG0080048073.01.T01:three_prime_UTR | 45.0% | |
| GCAGAAAAGTAAGGAGGATC+TGG | - | contig192end:5567-5586 | MsG0080048073.01.T01:intron | 45.0% | |
| GGAACTGGTGACAGTAAAGT+AGG | + | contig192end:5007-5026 | None:intergenic | 45.0% | |
| GGAGGAAGAGGAACATGTTT+AGG | + | contig192end:4750-4769 | None:intergenic | 45.0% | |
| GTCTATGTTTCTCACCTCTC+TGG | - | contig192end:5912-5931 | MsG0080048073.01.T01:CDS | 45.0% | |
| GTTGTAGGTTTCGGGAAAAG+TGG | + | contig192end:4810-4829 | None:intergenic | 45.0% | |
| TCCAGGAAGTAAAATGTCGC+CGG | - | contig192end:4956-4975 | MsG0080048073.01.T01:intron | 45.0% | |
| TTGACATCTCTAACTGGTGG+AGG | - | contig192end:4367-4386 | MsG0080048073.01.T01:three_prime_UTR | 45.0% | |
| ! | AATGGCAGAAGTGTAGTTCC+TGG | - | contig192end:4882-4901 | MsG0080048073.01.T01:CDS | 45.0% |
| ! | AGAAGTGTAGTTCCTGGTGT+TGG | - | contig192end:4888-4907 | MsG0080048073.01.T01:CDS | 45.0% |
| ! | CTCCCTCTGTTGATTGCATA+AGG | + | contig192end:5028-5047 | None:intergenic | 45.0% |
| ! | GCATTGACATCTCTAACTGG+TGG | - | contig192end:4364-4383 | MsG0080048073.01.T01:three_prime_UTR | 45.0% |
| !! | CTACCACCATTTGGGTTGTT+AGG | + | contig192end:4406-4425 | None:intergenic | 45.0% |
| AGCCTCCAGTTCAACAGTGT+AGG | + | contig192end:5386-5405 | None:intergenic | 50.0% | |
| GAAGGACACTTACAGGTCCA+TGG | - | contig192end:5617-5636 | MsG0080048073.01.T01:intron | 50.0% | |
| GGACCTAACAACCCAAATGG+TGG | - | contig192end:4400-4419 | MsG0080048073.01.T01:three_prime_UTR | 50.0% | |
| GTAGGTTTCGGGAAAAGTGG+TGG | + | contig192end:4807-4826 | None:intergenic | 50.0% | |
| TCTCTAACTGGTGGAGGAGT+AGG | - | contig192end:4373-4392 | MsG0080048073.01.T01:three_prime_UTR | 50.0% | |
| ! | ACAGCTCCTGTTACTTCTCC+AGG | - | contig192end:4939-4958 | MsG0080048073.01.T01:intron | 50.0% |
| ! | GTTCCTGGTGTTGGAATGCA+TGG | - | contig192end:4897-4916 | MsG0080048073.01.T01:CDS | 50.0% |
| ! | TCCGGCGACATTTTACTTCC+TGG | + | contig192end:4960-4979 | None:intergenic | 50.0% |
| !!! | GAGGAGTTTTTGGTGAGAGC+TGG | - | contig192end:4562-4581 | MsG0080048073.01.T01:three_prime_UTR | 50.0% |
| ACTGGTGGAGGAGTAGGAGA+AGG | - | contig192end:4379-4398 | MsG0080048073.01.T01:three_prime_UTR | 55.0% | |
| AGAATCAGCTGCGAGGTCAC+GGG | - | contig192end:5108-5127 | MsG0080048073.01.T01:intron | 55.0% | |
| GAGAATCAGCTGCGAGGTCA+CGG | - | contig192end:5107-5126 | MsG0080048073.01.T01:intron | 55.0% | |
| GGCCTACACTGTTGAACTGG+AGG | - | contig192end:5381-5400 | MsG0080048073.01.T01:intron | 55.0% | |
| GGTTTCGGGAAAAGTGGTGG+CGG | + | contig192end:4804-4823 | None:intergenic | 55.0% | |
| ! | AGACAGTCTAACAGGGGCAC+AGG | + | contig192end:5896-5915 | None:intergenic | 55.0% |
| ! | AGTCTAACAGGGGCACAGGC+AGG | + | contig192end:5892-5911 | None:intergenic | 60.0% |
| GAGGTCACGGGCTCGCAAAC+AGG | - | contig192end:5120-5139 | MsG0080048073.01.T01:intron | 65.0% | |
| ! | TAACAGGGGCACAGGCAGGC+TGG | + | contig192end:5888-5907 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig192end | gene | 4319 | 5984 | 4319 | ID=MsG0080048073.01;Name=MsG0080048073.01 |
| contig192end | mRNA | 4319 | 5984 | 4319 | ID=MsG0080048073.01.T01;Parent=MsG0080048073.01;Name=MsG0080048073.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0.66|0.5|1|1|1|4|342|203 |
| contig192end | exon | 4319 | 4753 | 4319 | ID=MsG0080048073.01.T01:exon:20053;Parent=MsG0080048073.01.T01 |
| contig192end | exon | 4850 | 4921 | 4850 | ID=MsG0080048073.01.T01:exon:20052;Parent=MsG0080048073.01.T01 |
| contig192end | exon | 5162 | 5280 | 5162 | ID=MsG0080048073.01.T01:exon:20051;Parent=MsG0080048073.01.T01 |
| contig192end | exon | 5657 | 5984 | 5657 | ID=MsG0080048073.01.T01:exon:20050;Parent=MsG0080048073.01.T01 |
| contig192end | CDS | 5657 | 5984 | 5657 | ID=MsG0080048073.01.T01:cds;Parent=MsG0080048073.01.T01 |
| contig192end | CDS | 5162 | 5280 | 5162 | ID=MsG0080048073.01.T01:cds;Parent=MsG0080048073.01.T01 |
| contig192end | CDS | 4850 | 4921 | 4850 | ID=MsG0080048073.01.T01:cds;Parent=MsG0080048073.01.T01 |
| contig192end | CDS | 4661 | 4753 | 4661 | ID=MsG0080048073.01.T01:cds;Parent=MsG0080048073.01.T01 |
| contig192end | three_prime_UTR | 4319 | 4660 | 4319 | ID=MsG0080048073.01.T01:three_prime_utr;Parent=MsG0080048073.01.T01 |
| Gene Sequence |
| Protein sequence |
