AlfalfaGEDB | MsG0880046569.01

M. sativa cultivar ZhongmuNo.1 / MsG0880046569.01

KEGG: K08856
GO: GO:0004672 GO:0005524 GO:0006468
PFAM: PF00069

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046569.01.T01	XP_003609185.1	79.198	399	24	4	1	397	1	342	0	616

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046569.01.T01	O88697	31.068	309	162	8	83	391	36	293	1.32E-39	146

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046569.01.T01	G7JU40	79.198	399	24	4	1	397	1	342	0.0	616

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048073.01	MsG0880046569.01	-0.807234	5.584327e-50	3.093907e-47
MsG0080048683.01	MsG0880046569.01	-0.809618	1.731131e-50	1.021358e-47
MsG0080048748.01	MsG0880046569.01	-0.834504	2.885689e-56	3.431450e-53
MsG0080048761.01	MsG0880046569.01	0.832434	9.472923e-56	1.058674e-52
MsG0080048849.01	MsG0880046569.01	-0.856486	3.115463e-62	7.448697e-59
MsG0080048878.01	MsG0880046569.01	-0.806113	9.632117e-50	5.184857e-47
MsG0080048938.01	MsG0880046569.01	-0.829467	5.063783e-55	5.187033e-52
MsG0080049069.01	MsG0880046569.01	-0.817583	3.058975e-52	2.236524e-49
MsG0180000008.01	MsG0880046569.01	-0.802915	4.471526e-49	2.217220e-46
MsG0180000026.01	MsG0880046569.01	-0.816473	5.431835e-52	3.852441e-49
MsG0180000599.01	MsG0880046569.01	-0.811489	6.825024e-51	4.230550e-48
MsG0180001722.01	MsG0880046569.01	-0.806643	7.445547e-50	4.062226e-47
MsG0180002184.01	MsG0880046569.01	-0.814731	1.326925e-51	8.974284e-49
MsG0180002700.01	MsG0880046569.01	-0.816865	4.437276e-52	3.181146e-49
MsG0880045285.01	MsG0880046569.01	-0.857364	1.716590e-62	4.226339e-59
MsG0880045765.01	MsG0880046569.01	-0.802520	5.396051e-49	2.648664e-46
MsG0880045783.01	MsG0880046569.01	-0.802808	4.705784e-49	2.327091e-46
MsG0880045992.01	MsG0880046569.01	-0.847072	1.457668e-59	2.560767e-56
MsG0880046334.01	MsG0880046569.01	0.840851	6.777259e-58	9.778442e-55
MsG0880046434.01	MsG0880046569.01	0.819190	1.322758e-52	1.010887e-49
MsG0880046569.01	MsG0880047045.01	-0.826035	3.383836e-54	3.137171e-51
MsG0880046569.01	MsG0880047046.01	-0.835555	1.567322e-56	1.923225e-53
MsG0880046569.01	MsG0880047052.01	-0.808807	2.583523e-50	1.491438e-47
MsG0880046569.01	MsG0880047481.01	0.848880	4.621883e-60	8.605006e-57
MsG0180003189.01	MsG0880046569.01	-0.802597	5.202887e-49	2.558984e-46
MsG0180004025.01	MsG0880046569.01	-0.825742	3.970403e-54	3.650582e-51
MsG0180005339.01	MsG0880046569.01	-0.807052	6.101336e-50	3.364415e-47
MsG0180005418.01	MsG0880046569.01	-0.802464	5.540639e-49	2.715906e-46
MsG0180006096.01	MsG0880046569.01	-0.800120	1.672878e-48	7.726677e-46
MsG0280006306.01	MsG0880046569.01	0.876336	1.513678e-68	7.310751e-65
MsG0280006318.01	MsG0880046569.01	0.831191	1.919642e-55	2.067891e-52
MsG0280006418.01	MsG0880046569.01	0.809061	2.278875e-50	1.324589e-47
MsG0780040295.01	MsG0880046569.01	0.826856	2.155949e-54	2.046179e-51
MsG0780040566.01	MsG0880046569.01	0.885548	7.394369e-72	5.096824e-68
MsG0780040763.01	MsG0880046569.01	-0.819728	9.973986e-53	7.737741e-50
MsG0780041491.01	MsG0880046569.01	-0.820394	7.020743e-53	5.548703e-50
MsG0780041729.01	MsG0880046569.01	0.820238	7.624062e-53	5.998994e-50
MsG0780041747.01	MsG0880046569.01	-0.853534	2.242400e-61	4.860802e-58
MsG0880041852.01	MsG0880046569.01	-0.831711	1.429698e-55	1.564289e-52
MsG0880042022.01	MsG0880046569.01	-0.829421	5.196231e-55	5.315697e-52
MsG0880042987.01	MsG0880046569.01	0.877990	4.031744e-69	2.069612e-65
MsG0880043024.01	MsG0880046569.01	-0.806178	9.330035e-50	5.030854e-47
MsG0280010564.01	MsG0880046569.01	-0.803666	3.126692e-49	1.580407e-46
MsG0280010604.01	MsG0880046569.01	0.825682	4.102437e-54	3.765477e-51
MsG0280011059.01	MsG0880046569.01	0.823453	1.373882e-53	1.183408e-50
MsG0280011180.01	MsG0880046569.01	-0.804564	2.032953e-49	1.051601e-46
MsG0280011255.01	MsG0880046569.01	-0.824554	7.580095e-54	6.737077e-51
MsG0380012005.01	MsG0880046569.01	-0.831952	1.246514e-55	1.373568e-52
MsG0380012868.01	MsG0880046569.01	-0.816732	4.752292e-52	3.394536e-49
MsG0380014240.01	MsG0880046569.01	-0.801176	1.018835e-48	4.833570e-46
MsG0380014287.01	MsG0880046569.01	-0.818973	1.481848e-52	1.125877e-49
MsG0680031334.01	MsG0880046569.01	-0.817694	2.887842e-52	2.117896e-49
MsG0680032827.01	MsG0880046569.01	-0.839184	1.844540e-57	2.527405e-54
MsG0680032830.01	MsG0880046569.01	-0.808248	3.399779e-50	1.934338e-47
MsG0680033459.01	MsG0880046569.01	-0.835188	1.940639e-56	2.355408e-53
MsG0680034759.01	MsG0880046569.01	0.868127	8.222539e-66	2.941562e-62
MsG0480019384.01	MsG0880046569.01	-0.811368	7.250211e-51	4.479525e-48
MsG0480019408.01	MsG0880046569.01	-0.840699	7.429198e-58	1.066859e-54
MsG0480020024.01	MsG0880046569.01	-0.803210	3.886611e-49	1.941692e-46
MsG0480020322.01	MsG0880046569.01	-0.829390	5.287886e-55	5.404734e-52
MsG0480020323.01	MsG0880046569.01	-0.814858	1.243382e-51	8.437911e-49
MsG0480021201.01	MsG0880046569.01	-0.813825	2.104265e-51	1.388636e-48
MsG0480021776.01	MsG0880046569.01	0.860962	1.432181e-63	3.985918e-60
MsG0480022624.01	MsG0880046569.01	-0.822738	2.017579e-53	1.703283e-50
MsG0480022739.01	MsG0880046569.01	0.836128	1.121765e-56	1.400592e-53
MsG0480022872.01	MsG0880046569.01	0.865027	7.954468e-65	2.550966e-61
MsG0480023348.01	MsG0880046569.01	0.838005	3.717984e-57	4.914866e-54
MsG0480023660.01	MsG0880046569.01	-0.824681	7.074972e-54	6.311641e-51
MsG0480023877.01	MsG0880046569.01	-0.826104	3.257273e-54	3.025792e-51
MsG0580024285.01	MsG0880046569.01	-0.832130	1.127002e-55	1.248146e-52
MsG0580024775.01	MsG0880046569.01	-0.806661	7.381995e-50	4.029408e-47
MsG0580024788.01	MsG0880046569.01	-0.819905	9.084346e-53	7.082667e-50
MsG0580024930.01	MsG0880046569.01	0.809934	1.480230e-50	8.805776e-48
MsG0580025132.01	MsG0880046569.01	-0.811458	6.932862e-51	4.293808e-48
MsG0580025467.01	MsG0880046569.01	-0.847981	8.196714e-60	1.482190e-56
MsG0580025827.01	MsG0880046569.01	-0.801733	7.831831e-49	3.768102e-46
MsG0580025832.01	MsG0880046569.01	-0.807772	4.293889e-50	2.412467e-47
MsG0580025906.01	MsG0880046569.01	-0.820172	7.892059e-53	6.199106e-50
MsG0380015372.01	MsG0880046569.01	-0.803398	3.553979e-49	1.784119e-46
MsG0380015847.01	MsG0880046569.01	0.857477	1.589059e-62	3.927173e-59
MsG0380015849.01	MsG0880046569.01	0.813576	2.386294e-51	1.564230e-48
MsG0380015891.01	MsG0880046569.01	-0.810819	9.535701e-51	5.806895e-48
MsG0380016191.01	MsG0880046569.01	0.806216	9.160661e-50	4.944333e-47
MsG0380016347.01	MsG0880046569.01	0.800683	1.284412e-48	6.017798e-46
MsG0380017263.01	MsG0880046569.01	0.807686	4.477679e-50	2.510076e-47
MsG0380017338.01	MsG0880046569.01	-0.854162	1.479013e-61	3.273857e-58
MsG0380017437.01	MsG0880046569.01	0.867616	1.200195e-65	4.216823e-62
MsG0480018426.01	MsG0880046569.01	-0.876261	1.606814e-68	7.738372e-65
MsG0580028033.01	MsG0880046569.01	-0.832966	6.991438e-56	7.938622e-53
MsG0580028551.01	MsG0880046569.01	-0.831607	1.516931e-55	1.654468e-52
MsG0580028901.01	MsG0880046569.01	-0.802098	6.590923e-49	3.200701e-46
MsG0580029001.01	MsG0880046569.01	-0.808540	2.945836e-50	1.688836e-47
MsG0580029514.01	MsG0880046569.01	0.834429	3.012387e-56	3.574090e-53
MsG0580029734.01	MsG0880046569.01	-0.802593	5.212731e-49	2.563599e-46
MsG0580029925.01	MsG0880046569.01	-0.803033	4.228086e-49	2.102698e-46
MsG0680030514.01	MsG0880046569.01	-0.809909	1.498779e-50	8.910233e-48
MsG0680030636.01	MsG0880046569.01	-0.803117	4.063118e-49	2.024952e-46
MsG0280006753.01	MsG0880046569.01	0.828146	1.057344e-54	1.042078e-51
MsG0280006914.01	MsG0880046569.01	-0.806510	7.943777e-50	4.319583e-47
MsG0280007647.01	MsG0880046569.01	-0.836815	7.502155e-57	9.562146e-54
MsG0280008098.01	MsG0880046569.01	-0.824615	7.332142e-54	6.528642e-51
MsG0280008660.01	MsG0880046569.01	0.841465	4.675720e-58	6.876456e-55
MsG0280008928.01	MsG0880046569.01	-0.848980	4.336998e-60	8.100851e-57
MsG0280008929.01	MsG0880046569.01	-0.838466	2.828547e-57	3.791807e-54
MsG0280009685.01	MsG0880046569.01	-0.810360	1.198482e-50	7.209299e-48
MsG0280009699.01	MsG0880046569.01	-0.802055	6.727448e-49	3.263446e-46
MsG0280010288.01	MsG0880046569.01	-0.825054	5.778001e-54	5.209496e-51
MsG0680035707.01	MsG0880046569.01	-0.803093	4.109690e-49	2.046853e-46
MsG0680035765.01	MsG0880046569.01	-0.800319	1.523807e-48	7.074062e-46
MsG0680035859.01	MsG0880046569.01	-0.801156	1.028553e-48	4.877291e-46
MsG0780035960.01	MsG0880046569.01	-0.806880	6.634135e-50	3.641968e-47
MsG0780036803.01	MsG0880046569.01	-0.838393	2.953342e-57	3.950245e-54
MsG0780036978.01	MsG0880046569.01	-0.809506	1.829602e-50	1.076154e-47
MsG0780037052.01	MsG0880046569.01	0.804122	2.513945e-49	1.285716e-46
MsG0780037105.01	MsG0880046569.01	-0.822840	1.910198e-53	1.617240e-50
MsG0780037330.01	MsG0880046569.01	-0.806965	6.366572e-50	3.502700e-47
MsG0780037506.01	MsG0880046569.01	0.851950	6.356299e-61	1.307485e-57
MsG0780038473.01	MsG0880046569.01	0.852816	3.601003e-61	7.619427e-58
MsG0780038783.01	MsG0880046569.01	-0.831266	1.840581e-55	1.987025e-52

PPI

Gene1	Gene2	Type
MsG0880046569.01	MsG0480018228.01	PPI
MsG0380017995.01	MsG0880046569.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046569.01.T01	MTR_4g112900	79.198	399	24	4	1	397	1	342	0.0	616
MsG0880046569.01.T01	MTR_3g026250	25.146	342	189	10	59	392	15	297	5.30e-19	89.4
MsG0880046569.01.T01	MTR_8g088590	24.681	235	157	9	170	397	224	445	1.46e-11	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046569.01.T01	AT5G08160	70.000	400	64	6	1	397	1	347	0.0	545
MsG0880046569.01.T01	AT5G08160	76.699	309	36	2	90	397	38	311	8.07e-171	479
MsG0880046569.01.T01	AT5G08160	96.667	30	1	0	1	30	1	30	5.09e-13	69.7
MsG0880046569.01.T01	AT2G32850	27.815	302	159	10	93	392	54	298	2.27e-19	90.5
MsG0880046569.01.T01	AT2G32850	27.815	302	159	10	93	392	54	298	3.65e-19	90.1

Find 128 sgRNAs with CRISPR-Local

Find 278 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTCTTGGCTTTCATTGTTT+TGG	0.155584	8:+74990526	None:intergenic
CTGGCCATACTGATGGATTT+TGG	0.264286	8:-74989992	MsG0880046569.01.T01:CDS
AAATATTTCGGCAGGTAAAA+AGG	0.275135	8:-74990475	MsG0880046569.01.T01:CDS
GGTGGAATAGTGTTCCTTCT+TGG	0.288284	8:+74990511	None:intergenic
ATATGTTTGGCACTGAAATT+AGG	0.295793	8:+74992148	None:intergenic
CTTGTTATTTCCAGTTCATT+TGG	0.299548	8:-74990570	MsG0880046569.01.T01:CDS
CAGATGTTCTTCAAATATTT+CGG	0.304963	8:-74990487	MsG0880046569.01.T01:CDS
CCGGGGCCTCATAGCAGCTT+GGG	0.324444	8:+74988261	None:intergenic
GAGCTAATCTTCTTCCTTGC+GGG	0.327554	8:+74989959	None:intergenic
GTCTCTTGTAATTTATGAAA+TGG	0.332989	8:-74987964	MsG0880046569.01.T01:three_prime_UTR
TCAAGAAGTGGGAGGAGATT+GGG	0.348271	8:+74990754	None:intergenic
CGGTACGAGAGCTCCTTTCT+TGG	0.351708	8:+74992274	None:intergenic
CATTTACAATAGCCAGTTGC+AGG	0.356717	8:+74988362	None:intergenic
AACCAAGAATGACTATAATA+AGG	0.364039	8:+74990338	None:intergenic
CAACTGGAGTGCCTCTGATC+TGG	0.364109	8:+74989937	None:intergenic
ATCAGAGGCACTCCAGTTGC+AGG	0.377493	8:-74989933	MsG0880046569.01.T01:intron
TGGATCTTATGCTATGAAAA+AGG	0.382382	8:-74990848	MsG0880046569.01.T01:CDS
CTTGGGCAATCCCACAGCTC+AGG	0.394165	8:+74989434	None:intergenic
TGCTTACGTTTATCTCGTTA+AGG	0.398078	8:-74991958	MsG0880046569.01.T01:CDS
GGATAATGATATCGTCTATC+CGG	0.398152	8:+74988242	None:intergenic
GAAATAACAAGTACGCTTCA+TGG	0.400033	8:+74990582	None:intergenic
ACGAACTGATGAAGAGCTTC+TGG	0.403819	8:+74988301	None:intergenic
ACGAGCAGCTGGAGTTGGTC+AGG	0.404228	8:-74990810	MsG0880046569.01.T01:CDS
TCCGGGGCCTCATAGCAGCT+TGG	0.408265	8:+74988260	None:intergenic
TCAACCGACGACGTGAAATC+AGG	0.416104	8:-74992195	MsG0880046569.01.T01:five_prime_UTR
CCCCTTCTCCGAGCTGCCTC+AGG	0.422367	8:+74991984	None:intergenic
ATCAAGAAGTGGGAGGAGAT+TGG	0.424760	8:+74990753	None:intergenic
TGAACGGAGGTGGTGATGTT+TGG	0.427535	8:-74992030	MsG0880046569.01.T01:CDS
TGACTATAATAAGGTCTAAA+CGG	0.428264	8:+74990347	None:intergenic
ATCATATAATGCATTTAAAC+CGG	0.431617	8:+74992057	None:intergenic
TCATGCAATCATATCTGTTA+AGG	0.433133	8:-74990731	MsG0880046569.01.T01:intron
CCACCGGTAGCAGAGTCATT+AGG	0.436423	8:+74991930	None:intergenic
CTCCGAGCTGCCTCAGGATG+CGG	0.439204	8:+74991990	None:intergenic
TTGGTGTTAGTCTAGATAGT+AGG	0.440649	8:-74988032	MsG0880046569.01.T01:three_prime_UTR
AATCCCACAGCTCAGGGGCT+CGG	0.444654	8:+74989441	None:intergenic
AAGAATGAATCACCTGCAAC+TGG	0.444808	8:+74989921	None:intergenic
ATCCTGAGGCAGCTCGGAGA+AGG	0.445375	8:-74991986	MsG0880046569.01.T01:CDS
GTCTGGTGGAAGCCTGCAAC+TGG	0.446847	8:-74988374	MsG0880046569.01.T01:CDS
AGTAGACACACACATGTGTT+TGG	0.447137	8:-74992313	MsG0880046569.01.T01:five_prime_UTR
TGAGGAAGACACAGGGAGAT+TGG	0.447172	8:+74987941	None:intergenic
TCCTGAGGCAGCTCGGAGAA+GGG	0.450906	8:-74991985	MsG0880046569.01.T01:CDS
TTGTAAATGCTCAGGTCAAA+TGG	0.451429	8:-74988348	MsG0880046569.01.T01:CDS
TTGGGCAATCCCACAGCTCA+GGG	0.451475	8:+74989435	None:intergenic
CATCTATATCAGCATGGCTT+GGG	0.452595	8:+74989417	None:intergenic
TTATTTCCAGTTCATTTGGA+TGG	0.458073	8:-74990566	MsG0880046569.01.T01:CDS
CAGAAAAGGACAACCACCAC+TGG	0.459960	8:-74990011	MsG0880046569.01.T01:CDS
GAATATGCACTTGGAGAGTC+TGG	0.460612	8:-74988391	MsG0880046569.01.T01:CDS
AGGACTGACGTTTGGTCACT+AGG	0.464707	8:-74989392	MsG0880046569.01.T01:CDS
CTTTCCGAGCCCCTGAGCTG+TGG	0.468523	8:-74989445	MsG0880046569.01.T01:CDS
AATGTTCCATCCAAATGAAC+TGG	0.469154	8:+74990560	None:intergenic
CATTCAGAACAACGAGCAGC+TGG	0.475734	8:-74990821	MsG0880046569.01.T01:CDS
ACTGCCAAAATCCATCAGTA+TGG	0.479140	8:+74989988	None:intergenic
CAAGAAGTGGGAGGAGATTG+GGG	0.480551	8:+74990755	None:intergenic
TGGTGTTAGTCTAGATAGTA+GGG	0.480943	8:-74988031	MsG0880046569.01.T01:three_prime_UTR
TCATCTATATCAGCATGGCT+TGG	0.481050	8:+74989416	None:intergenic
TAGATGAGAGGACTGACGTT+TGG	0.482745	8:-74989400	MsG0880046569.01.T01:CDS
CCCAAGCTGCTATGAGGCCC+CGG	0.483526	8:-74988261	MsG0880046569.01.T01:CDS
GCTCAGGTCAAATGGCCAGC+TGG	0.486173	8:-74988340	MsG0880046569.01.T01:CDS
AAAATGGGTTGCTCATTCTC+CGG	0.492235	8:-74992076	MsG0880046569.01.T01:CDS
TTTCCGAGCCCCTGAGCTGT+GGG	0.494385	8:-74989444	MsG0880046569.01.T01:CDS
TGAAGAGCTTCTGGATATGA+AGG	0.497130	8:+74988310	None:intergenic
GAACAACGAGCAGCTGGAGT+TGG	0.499688	8:-74990815	MsG0880046569.01.T01:CDS
AGTCTAGATAGTAGGGCGTT+GGG	0.502257	8:-74988024	MsG0880046569.01.T01:three_prime_UTR
TAGTCTAGATAGTAGGGCGT+TGG	0.503563	8:-74988025	MsG0880046569.01.T01:three_prime_UTR
TTCAGCCCCAAGCTGCTATG+AGG	0.508302	8:-74988267	MsG0880046569.01.T01:CDS
TGTTCTTCAAATATTTCGGC+AGG	0.511807	8:-74990483	MsG0880046569.01.T01:CDS
GCAAACAGAGTGAGAAATAG+AGG	0.515649	8:-74992111	MsG0880046569.01.T01:five_prime_UTR
GTTCTCATAACACACAGAAA+AGG	0.516035	8:-74990025	MsG0880046569.01.T01:CDS
CCTGAGGCAGCTCGGAGAAG+GGG	0.516411	8:-74991984	MsG0880046569.01.T01:CDS
ACCACCACTGGCCATACTGA+TGG	0.518489	8:-74989999	MsG0880046569.01.T01:CDS
ACTGGCTATTGTAAATGCTC+AGG	0.518915	8:-74988356	MsG0880046569.01.T01:CDS
CTCTTCATCAGTTCGTGTCA+TGG	0.519184	8:-74988294	MsG0880046569.01.T01:CDS
GATAATGATATCGTCTATCC+GGG	0.519411	8:+74988243	None:intergenic
AGCCTACTCAAGAAACATCT+TGG	0.522960	8:-74990607	MsG0880046569.01.T01:CDS
CTCAATGAGTTATCATCGTA+GGG	0.525641	8:-74988192	MsG0880046569.01.T01:exon
AAAATCGAGCGATCCAAGAA+AGG	0.529945	8:-74992287	MsG0880046569.01.T01:five_prime_UTR
TTGACATCATTGTGAGCGTA+AGG	0.531572	8:+74990058	None:intergenic
TTCCGCATCCTGAGGCAGCT+CGG	0.538095	8:-74991992	MsG0880046569.01.T01:CDS
GGAGCTAATCTTCTTCCTTG+CGG	0.539998	8:+74989958	None:intergenic
CAAAACAATGAAAGCCAAGA+AGG	0.541448	8:-74990525	MsG0880046569.01.T01:CDS
TCTCAATGAGTTATCATCGT+AGG	0.547768	8:-74988193	MsG0880046569.01.T01:exon
CACAGCTCAGGGGCTCGGAA+AGG	0.548026	8:+74989446	None:intergenic
TCTCCAAGTGCATATTCAAA+CGG	0.554376	8:+74988397	None:intergenic
TATGAAGGTTTAAGTCCAGC+TGG	0.554831	8:+74988325	None:intergenic
GCATTATATGATTCTGTGAA+CGG	0.557472	8:-74992046	MsG0880046569.01.T01:CDS
TCAACTCAACAGCTTTGTGC+AGG	0.560174	8:-74990109	MsG0880046569.01.T01:CDS
AAATAACAAGTACGCTTCAT+GGG	0.562791	8:+74990583	None:intergenic
CGGGGCCTCATAGCAGCTTG+GGG	0.569578	8:+74988262	None:intergenic
AAAGACTCTTCTCATCTCGC+AGG	0.571736	8:-74991891	MsG0880046569.01.T01:intron
TCTCCGTTTGAATATGCACT+TGG	0.573973	8:-74988400	MsG0880046569.01.T01:CDS
CGAGCAGCTGGAGTTGGTCA+GGG	0.581004	8:-74990809	MsG0880046569.01.T01:CDS
TATGATTCTGTGAACGGAGG+TGG	0.581095	8:-74992040	MsG0880046569.01.T01:CDS
ACAGATACGTTTAACTCTGT+GGG	0.581766	8:+74988079	None:intergenic
TGGGCAATCCCACAGCTCAG+GGG	0.583786	8:+74989436	None:intergenic
AAGACCTGATTTCACGTCGT+CGG	0.586470	8:+74992191	None:intergenic
GCTGCTCGTTGTTCTGAATG+AGG	0.586598	8:+74990824	None:intergenic
GTCCAAGATGTTTCTTGAGT+AGG	0.586917	8:+74990605	None:intergenic
GCTCCTAATGACTCTGCTAC+CGG	0.588613	8:-74991933	MsG0880046569.01.T01:CDS
GAGCTTATTAGCGAGACCAC+CGG	0.590187	8:+74991914	None:intergenic
ATGATTGCATGATCAAGAAG+TGG	0.592500	8:+74990742	None:intergenic
TGTGTTATGAGAACATTACC+AGG	0.596220	8:+74990034	None:intergenic
TTGGCAGTGCTCGTCCCGCA+AGG	0.600540	8:-74989973	MsG0880046569.01.T01:CDS
TACAGATACGTTTAACTCTG+TGG	0.605114	8:+74988078	None:intergenic
TCCTCTCATCTATATCAGCA+TGG	0.618760	8:+74989411	None:intergenic
TCCATCAGTATGGCCAGTGG+TGG	0.622023	8:+74989998	None:intergenic
TGATTGCATGATCAAGAAGT+GGG	0.623676	8:+74990743	None:intergenic
GAAGATTAGCTCCAGATCAG+AGG	0.625557	8:-74989948	MsG0880046569.01.T01:CDS
GCCATGCTGATATAGATGAG+AGG	0.628216	8:-74989412	MsG0880046569.01.T01:CDS
ACATCATTGTGAGCGTAAGG+AGG	0.628315	8:+74990061	None:intergenic
ATTTATGCGCATAAAATCTG+AGG	0.630471	8:+74987923	None:intergenic
ATAAAATCTGAGGAAGACAC+AGG	0.635750	8:+74987933	None:intergenic
GCTCACAATGATGTCAAACC+TGG	0.640645	8:-74990052	MsG0880046569.01.T01:CDS
TTGCAATCAACTTATCAACA+TGG	0.641794	8:+74988221	None:intergenic
TCGTAGGGAACAGTGTGGCA+TGG	0.643236	8:-74988177	MsG0880046569.01.T01:three_prime_UTR
TATTTGAAGAACATCTGAGG+TGG	0.650365	8:+74990493	None:intergenic
AGAATCGATTCCGCATCCTG+AGG	0.654930	8:-74992000	MsG0880046569.01.T01:CDS
CCTAATGACTCTGCTACCGG+TGG	0.657390	8:-74991930	MsG0880046569.01.T01:CDS
AAATATTTGAAGAACATCTG+AGG	0.664166	8:+74990490	None:intergenic
GCAGCTGGAGTTGGTCAGGG+AGG	0.666966	8:-74990806	MsG0880046569.01.T01:CDS
TATGCACTTGGAGAGTCTGG+TGG	0.682888	8:-74988388	MsG0880046569.01.T01:CDS
GACTAAACAGTGAAGAGACG+CGG	0.691998	8:+74990779	None:intergenic
TTGCATGATCAAGAAGTGGG+AGG	0.694089	8:+74990746	None:intergenic
TAAAATCTGAGGAAGACACA+GGG	0.695867	8:+74987934	None:intergenic
AAATCCATCAGTATGGCCAG+TGG	0.697664	8:+74989995	None:intergenic
AATTGCAAAAGTGATGCTTG+AGG	0.708820	8:+74990386	None:intergenic
ATAATGATATCGTCTATCCG+GGG	0.742304	8:+74988244	None:intergenic
TTATATGATTCTGTGAACGG+AGG	0.782200	8:-74992043	MsG0880046569.01.T01:CDS
TATCATCGTAGGGAACAGTG+TGG	0.794948	8:-74988182	MsG0880046569.01.T01:three_prime_UTR

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTTACAAACACTAAAAAAA+AGG	+	Chr8:74990295-74990314	None:intergenic	15.0%
!!!	CAAAGAAATTTAGTTTTAAA+TGG	+	Chr8:74990550-74990569	None:intergenic	15.0%
!!!	CATTTTTATTTATTACGATA+CGG	+	Chr8:74987939-74987958	None:intergenic	15.0%
!!!	TATAATTTCTTTTTTCATCA+GGG	-	Chr8:74989766-74989785	MsG0880046569.01.T01:intron	15.0%
!!!	TATGCATATATTATTGTTTT+GGG	-	Chr8:74991434-74991453	MsG0880046569.01.T01:intron	15.0%
!!!	TGTTTTTAAATGAATATAGA+TGG	-	Chr8:74988713-74988732	MsG0880046569.01.T01:intron	15.0%
!!!	TTATAATTTCTTTTTTCATC+AGG	-	Chr8:74989765-74989784	MsG0880046569.01.T01:intron	15.0%
!!!	TTTTGTATATGAAATATGTT+TGG	+	Chr8:74988058-74988077	None:intergenic	15.0%
!!!	TTTTTTTTTCTTTTCGTTTT+TGG	-	Chr8:74992139-74992158	MsG0880046569.01.T01:five_prime_UTR	15.0%
!!	AATCATTTGTACAAAAAGAA+TGG	-	Chr8:74989941-74989960	MsG0880046569.01.T01:CDS	20.0%
!!	ACAATTTCACATAATACAAA+TGG	+	Chr8:74990442-74990461	None:intergenic	20.0%
!!	ATCATATAATGCATTTAAAC+CGG	+	Chr8:74988136-74988155	None:intergenic	20.0%
!!	ATTTCTTAATTTACATTACC+TGG	-	Chr8:74990515-74990534	MsG0880046569.01.T01:CDS	20.0%
!!	CAAATATATGAAAACAATAC+TGG	+	Chr8:74991059-74991078	None:intergenic	20.0%
!!	CACATCTTAACAATTAAAAA+TGG	+	Chr8:74988341-74988360	None:intergenic	20.0%
!!	TATAAAATAAGAGAAAGGAA+GGG	-	Chr8:74991198-74991217	MsG0880046569.01.T01:intron	20.0%
!!	TGATTCTTTATTAATTCATG+TGG	-	Chr8:74991744-74991763	MsG0880046569.01.T01:intron	20.0%
!!!	AGCTTAATTTTGATAATAAG+TGG	-	Chr8:74988626-74988645	MsG0880046569.01.T01:intron	20.0%
!!!	ATAATTTCTTTTTTCATCAG+GGG	-	Chr8:74989767-74989786	MsG0880046569.01.T01:intron	20.0%
!!!	ATGCATATATTATTGTTTTG+GGG	-	Chr8:74991435-74991454	MsG0880046569.01.T01:intron	20.0%
!!!	CAGTATTGTTTTCATATATT+TGG	-	Chr8:74991057-74991076	MsG0880046569.01.T01:intron	20.0%
!!!	CTATGCATATATTATTGTTT+TGG	-	Chr8:74991433-74991452	MsG0880046569.01.T01:intron	20.0%
!!!	CTTATAACTATATCACATTT+TGG	-	Chr8:74990396-74990415	MsG0880046569.01.T01:intron	20.0%
!!!	TAGCAATTTTGAATTGAAAT+TGG	-	Chr8:74988797-74988816	MsG0880046569.01.T01:intron	20.0%
!!!	TGATTTATGTTTGAATGTTT+TGG	-	Chr8:74988865-74988884	MsG0880046569.01.T01:intron	20.0%
!	AAAAATTATCCCAACTCTAT+TGG	+	Chr8:74991599-74991618	None:intergenic	25.0%
!	AAACTTCTATACAAAGCTAT+TGG	+	Chr8:74991506-74991525	None:intergenic	25.0%
!	AAATATTTGAAGAACATCTG+AGG	+	Chr8:74989703-74989722	None:intergenic	25.0%
!	AAATCAAGCAATAATTCCAA+GGG	+	Chr8:74991560-74991579	None:intergenic	25.0%
!	AACCAAGAATGACTATAATA+AGG	+	Chr8:74989855-74989874	None:intergenic	25.0%
!	ACAATTAAAAATGGATCGAA+AGG	+	Chr8:74988332-74988351	None:intergenic	25.0%
!	ACATTATATGCAATAATGTG+AGG	-	Chr8:74990825-74990844	MsG0880046569.01.T01:CDS	25.0%
!	CAGATGTTCTTCAAATATTT+CGG	-	Chr8:74989703-74989722	MsG0880046569.01.T01:intron	25.0%
!	CTAAAATCTCAAAAAGAAGA+TGG	+	Chr8:74989998-74990017	None:intergenic	25.0%
!	CTATAAAATAAGAGAAAGGA+AGG	-	Chr8:74991197-74991216	MsG0880046569.01.T01:intron	25.0%
!	GTCTCTTGTAATTTATGAAA+TGG	-	Chr8:74992226-74992245	MsG0880046569.01.T01:five_prime_UTR	25.0%
!	TAGCCTATAAAATAAGAGAA+AGG	-	Chr8:74991193-74991212	MsG0880046569.01.T01:intron	25.0%
!	TGACTATAATAAGGTCTAAA+CGG	+	Chr8:74989846-74989865	None:intergenic	25.0%
!!	ACATAAAAAGCTTTTACTCT+TGG	+	Chr8:74988682-74988701	None:intergenic	25.0%
!!	CTTTTAGATATATAACTGGA+CGG	+	Chr8:74992075-74992094	None:intergenic	25.0%
!!	GAAAAAGAAAAGCTTCTTTT+CGG	-	Chr8:74990641-74990660	MsG0880046569.01.T01:intron	25.0%
!!	TCGTTAAGTAGAATCAATTT+TGG	+	Chr8:74989051-74989070	None:intergenic	25.0%
!!	TTGATTTTACGAGGTTAAAA+GGG	+	Chr8:74990065-74990084	None:intergenic	25.0%
!!	TTTTGTACAAATGATTCTCA+AGG	+	Chr8:74989937-74989956	None:intergenic	25.0%
!!!	ACTGCTTTTAGATATATAAC+TGG	+	Chr8:74992079-74992098	None:intergenic	25.0%
!!!	CTTCCTTTCTCTTATTTTAT+AGG	+	Chr8:74991199-74991218	None:intergenic	25.0%
!!!	TGATTTTTTTGATTTCCGAT+CGG	-	Chr8:74988433-74988452	MsG0880046569.01.T01:intron	25.0%
!!!	TTAGCAATGGATTGAAATTT+AGG	-	Chr8:74990991-74991010	MsG0880046569.01.T01:intron	25.0%
!!!	TTTTAAAATCTACACGAAAC+TGG	+	Chr8:74990617-74990636	None:intergenic	25.0%
	AAATAACAAGTACGCTTCAT+GGG	+	Chr8:74989610-74989629	None:intergenic	30.0%
	AAATATTTCGGCAGGTAAAA+AGG	-	Chr8:74989715-74989734	MsG0880046569.01.T01:intron	30.0%
	AACAGAAATCCAATAGAGTT+GGG	-	Chr8:74991587-74991606	MsG0880046569.01.T01:intron	30.0%
	ACAATTTGAATTAGTGACTC+TGG	+	Chr8:74991316-74991335	None:intergenic	30.0%
	ACACAAAAGATCAATTCTGA+TGG	-	Chr8:74989252-74989271	MsG0880046569.01.T01:intron	30.0%
	AGGATCAATATGCACATTAA+GGG	+	Chr8:74990966-74990985	None:intergenic	30.0%
	AGGATCTGAAGAAGAAAAAT+GGG	-	Chr8:74988099-74988118	MsG0880046569.01.T01:three_prime_UTR	30.0%
	AGTCAAATTACTGACATGTT+TGG	-	Chr8:74988997-74989016	MsG0880046569.01.T01:intron	30.0%
	ATATGAAAACAATACTGGCA+GGG	+	Chr8:74991054-74991073	None:intergenic	30.0%
	ATTTATGCGCATAAAATCTG+AGG	+	Chr8:74992270-74992289	None:intergenic	30.0%
	CAAATCAAGCAATAATTCCA+AGG	+	Chr8:74991561-74991580	None:intergenic	30.0%
	CTTGGAATTATTGCTTGATT+TGG	-	Chr8:74991559-74991578	MsG0880046569.01.T01:intron	30.0%
	GACCTTATTATAGTCATTCT+TGG	-	Chr8:74989850-74989869	MsG0880046569.01.T01:intron	30.0%
	GAGTAAAATGTAAAGTGACA+TGG	+	Chr8:74989158-74989177	None:intergenic	30.0%
	GATTCATTCATGTAAAAGTG+AGG	+	Chr8:74989130-74989149	None:intergenic	30.0%
	GCATTATATGATTCTGTGAA+CGG	-	Chr8:74988144-74988163	MsG0880046569.01.T01:three_prime_UTR	30.0%
	TAATTCATGACATGAGACTT+GGG	-	Chr8:74989902-74989921	MsG0880046569.01.T01:intron	30.0%
	TATATGAAAACAATACTGGC+AGG	+	Chr8:74991055-74991074	None:intergenic	30.0%
	TCATGCAATCATATCTGTTA+AGG	-	Chr8:74989459-74989478	MsG0880046569.01.T01:CDS	30.0%
	TGAAATTGTATTGCCTAATG+TGG	-	Chr8:74990453-74990472	MsG0880046569.01.T01:intron	30.0%
	TGGATCTTATGCTATGAAAA+AGG	-	Chr8:74989342-74989361	MsG0880046569.01.T01:intron	30.0%
	TTATTTCCAGTTCATTTGGA+TGG	-	Chr8:74989624-74989643	MsG0880046569.01.T01:intron	30.0%
	TTCAACAAATGACATAGATC+AGG	+	Chr8:74988370-74988389	None:intergenic	30.0%
	TTCAAGCAAGAGTTATTTGA+TGG	+	Chr8:74991671-74991690	None:intergenic	30.0%
	TTGCAATCAACTTATCAACA+TGG	+	Chr8:74991972-74991991	None:intergenic	30.0%
	TTTAAATGGAATGTAAGACC+AGG	+	Chr8:74990536-74990555	None:intergenic	30.0%
!	AAGTGATGAAAAGTAGCTTT+TGG	+	Chr8:74988930-74988949	None:intergenic	30.0%
!	AATGTAAAGTGACATGGTTT+TGG	+	Chr8:74989152-74989171	None:intergenic	30.0%
!	ACAAAAAGAATGGATTTCTC+TGG	-	Chr8:74989951-74989970	MsG0880046569.01.T01:CDS	30.0%
!	ACAAGATATGTTTCTAACAC+CGG	+	Chr8:74991703-74991722	None:intergenic	30.0%
!	AGTGATGAAAAGTAGCTTTT+GGG	+	Chr8:74988929-74988948	None:intergenic	30.0%
!	AGTGATTTTGTGATTAGACT+CGG	-	Chr8:74988526-74988545	MsG0880046569.01.T01:intron	30.0%
!	ATATGTTTGGCACTGAAATT+AGG	+	Chr8:74988045-74988064	None:intergenic	30.0%
!	CAGATTTTATGCGCATAAAT+GGG	-	Chr8:74992270-74992289	MsG0880046569.01.T01:five_prime_UTR	30.0%
!	CTTTGCTTGTGATTTCTTTT+TGG	-	Chr8:74990565-74990584	MsG0880046569.01.T01:CDS	30.0%
!	TCAGATTTTATGCGCATAAA+TGG	-	Chr8:74992269-74992288	MsG0880046569.01.T01:five_prime_UTR	30.0%
!	TCTCATCTACAAGAACATTT+TGG	+	Chr8:74991239-74991258	None:intergenic	30.0%
!	TGATCTTTTGTGTGTATGTT+TGG	+	Chr8:74989245-74989264	None:intergenic	30.0%
!	TTCATGTGGCTAAAATTTTC+AGG	-	Chr8:74991758-74991777	MsG0880046569.01.T01:intron	30.0%
!!	CTTGTTATTTCCAGTTCATT+TGG	-	Chr8:74989620-74989639	MsG0880046569.01.T01:intron	30.0%
!!	GGTCTTTGAAAATCACTTTT+TGG	-	Chr8:74988016-74988035	MsG0880046569.01.T01:three_prime_UTR	30.0%
!!	GTTGATTTTACGAGGTTAAA+AGG	+	Chr8:74990066-74990085	None:intergenic	30.0%
!!!	TGGCTAAAATTTTCAGGTAT+GGG	-	Chr8:74991764-74991783	MsG0880046569.01.T01:intron	30.0%
	AATGTTCCATCCAAATGAAC+TGG	+	Chr8:74989633-74989652	None:intergenic	35.0%
	AATTGCAAAAGTGATGCTTG+AGG	+	Chr8:74989807-74989826	None:intergenic	35.0%
	ACAGATACGTTTAACTCTGT+GGG	+	Chr8:74992114-74992133	None:intergenic	35.0%
	ATAAAATCTGAGGAAGACAC+AGG	+	Chr8:74992260-74992279	None:intergenic	35.0%
	ATAATGATATCGTCTATCCG+GGG	+	Chr8:74991949-74991968	None:intergenic	35.0%
	ATGATTGCATGATCAAGAAG+TGG	+	Chr8:74989451-74989470	None:intergenic	35.0%
	ATTGTATTGCCTAATGTGGT+AGG	-	Chr8:74990457-74990476	MsG0880046569.01.T01:intron	35.0%
	CAAAACAATGAAAGCCAAGA+AGG	-	Chr8:74989665-74989684	MsG0880046569.01.T01:intron	35.0%
	CAACAGAAATCCAATAGAGT+TGG	-	Chr8:74991586-74991605	MsG0880046569.01.T01:intron	35.0%
	CAGGATCAATATGCACATTA+AGG	+	Chr8:74990967-74990986	None:intergenic	35.0%
	CTCAATGAGTTATCATCGTA+GGG	-	Chr8:74991998-74992017	MsG0880046569.01.T01:CDS	35.0%
	CTTGCTTGAAAACAACTATC+CGG	-	Chr8:74991681-74991700	MsG0880046569.01.T01:intron	35.0%
	GAAATAACAAGTACGCTTCA+TGG	+	Chr8:74989611-74989630	None:intergenic	35.0%
	GAGGATCTGAAGAAGAAAAA+TGG	-	Chr8:74988098-74988117	MsG0880046569.01.T01:three_prime_UTR	35.0%
	GATAATGATATCGTCTATCC+GGG	+	Chr8:74991950-74991969	None:intergenic	35.0%
	GGATAATGATATCGTCTATC+CGG	+	Chr8:74991951-74991970	None:intergenic	35.0%
	GTAATTCATGACATGAGACT+TGG	-	Chr8:74989901-74989920	MsG0880046569.01.T01:intron	35.0%
	GTTCTCATAACACACAGAAA+AGG	-	Chr8:74990165-74990184	MsG0880046569.01.T01:intron	35.0%
	TAAAATCTGAGGAAGACACA+GGG	+	Chr8:74992259-74992278	None:intergenic	35.0%
	TACAGATACGTTTAACTCTG+TGG	+	Chr8:74992115-74992134	None:intergenic	35.0%
	TATTTGAAGAACATCTGAGG+TGG	+	Chr8:74989700-74989719	None:intergenic	35.0%
	TCTCAATGAGTTATCATCGT+AGG	-	Chr8:74991997-74992016	MsG0880046569.01.T01:CDS	35.0%
	TCTCCAAGTGCATATTCAAA+CGG	+	Chr8:74991796-74991815	None:intergenic	35.0%
	TCTTGGTTACTACAGCAAAA+TGG	+	Chr8:74988665-74988684	None:intergenic	35.0%
	TGATTGCATGATCAAGAAGT+GGG	+	Chr8:74989450-74989469	None:intergenic	35.0%
	TGCTTACGTTTATCTCGTTA+AGG	-	Chr8:74988232-74988251	MsG0880046569.01.T01:CDS	35.0%
	TGTCAGTAATTTGACTCCTT+TGG	+	Chr8:74988993-74989012	None:intergenic	35.0%
	TGTGTTATGAGAACATTACC+AGG	+	Chr8:74990159-74990178	None:intergenic	35.0%
	TGTTCTTCAAATATTTCGGC+AGG	-	Chr8:74989707-74989726	MsG0880046569.01.T01:intron	35.0%
	TTATATGATTCTGTGAACGG+AGG	-	Chr8:74988147-74988166	MsG0880046569.01.T01:three_prime_UTR	35.0%
	TTGTAAATGCTCAGGTCAAA+TGG	-	Chr8:74991842-74991861	MsG0880046569.01.T01:intron	35.0%
	TTGTAGATGAGATGTCAAGA+GGG	-	Chr8:74991247-74991266	MsG0880046569.01.T01:intron	35.0%
!	ATGTTTGGTTTCACTTCCAA+AGG	-	Chr8:74988974-74988993	MsG0880046569.01.T01:intron	35.0%
!	GAGTTATTTGATGGTACTGA+TGG	+	Chr8:74991662-74991681	None:intergenic	35.0%
!	GCTATTGGATTTTATTCACG+TGG	+	Chr8:74991491-74991510	None:intergenic	35.0%
!	GTGATGAAAAGTAGCTTTTG+GGG	+	Chr8:74988928-74988947	None:intergenic	35.0%
!	TGGTGTTAGTCTAGATAGTA+GGG	-	Chr8:74992159-74992178	MsG0880046569.01.T01:five_prime_UTR	35.0%
!	TTGTCTTGCTATGTAGGAAT+GGG	-	Chr8:74990701-74990720	MsG0880046569.01.T01:intron	35.0%
!!	CTACAAGAACATTTTGGTCA+GGG	+	Chr8:74991233-74991252	None:intergenic	35.0%
!!	CTTCTTGGCTTTCATTGTTT+TGG	+	Chr8:74989667-74989686	None:intergenic	35.0%
!!	TCTACAAGAACATTTTGGTC+AGG	+	Chr8:74991234-74991253	None:intergenic	35.0%
!!	TTGGTGTTAGTCTAGATAGT+AGG	-	Chr8:74992158-74992177	MsG0880046569.01.T01:five_prime_UTR	35.0%
!!!	ATTTCTTTTTTCATCAGGGG+AGG	-	Chr8:74989770-74989789	MsG0880046569.01.T01:intron	35.0%
!!!	GCTCTTGTTATTGCGTTTTA+TGG	-	Chr8:74988500-74988519	MsG0880046569.01.T01:intron	35.0%
!!!	GGCTAAAATTTTCAGGTATG+GGG	-	Chr8:74991765-74991784	MsG0880046569.01.T01:intron	35.0%
!!!	GTGGCTAAAATTTTCAGGTA+TGG	-	Chr8:74991763-74991782	MsG0880046569.01.T01:intron	35.0%
!!!	TTTCTTTTTTCATCAGGGGA+GGG	-	Chr8:74989771-74989790	MsG0880046569.01.T01:intron	35.0%
	AAAATCGAGCGATCCAAGAA+AGG	-	Chr8:74987903-74987922	MsG0880046569.01.T01:three_prime_UTR	40.0%
	AAGAATGAATCACCTGCAAC+TGG	+	Chr8:74990272-74990291	None:intergenic	40.0%
	AAGGGTTTAGATCAAGTGCA+TGG	+	Chr8:74990948-74990967	None:intergenic	40.0%
	ACTGCCAAAATCCATCAGTA+TGG	+	Chr8:74990205-74990224	None:intergenic	40.0%
	ACTGGCTATTGTAAATGCTC+AGG	-	Chr8:74991834-74991853	MsG0880046569.01.T01:intron	40.0%
	AGCCTACTCAAGAAACATCT+TGG	-	Chr8:74989583-74989602	MsG0880046569.01.T01:intron	40.0%
	CATCACTTCTAGTCAAACCA+AGG	-	Chr8:74988942-74988961	MsG0880046569.01.T01:intron	40.0%
	CATCTATATCAGCATGGCTT+GGG	+	Chr8:74990776-74990795	None:intergenic	40.0%
	CATTTACAATAGCCAGTTGC+AGG	+	Chr8:74991831-74991850	None:intergenic	40.0%
	CCAAACATCTTTCGAAACCT+TGG	+	Chr8:74988962-74988981	None:intergenic	40.0%
	CCAAGGTTTCGAAAGATGTT+TGG	-	Chr8:74988959-74988978	MsG0880046569.01.T01:intron	40.0%
	CCTCAAGAGTCAAGAAAACA+CGG	+	Chr8:74991531-74991550	None:intergenic	40.0%
	CTTGTAGATGAGATGTCAAG+AGG	-	Chr8:74991246-74991265	MsG0880046569.01.T01:intron	40.0%
	GAAAATTCAGCTTCACCGAT+CGG	+	Chr8:74988451-74988470	None:intergenic	40.0%
	GCAAACAGAGTGAGAAATAG+AGG	-	Chr8:74988079-74988098	MsG0880046569.01.T01:three_prime_UTR	40.0%
	GCATAGTGATCCAATAGCAT+GGG	+	Chr8:74991419-74991438	None:intergenic	40.0%
	GGACCAAAATGTGGTTATGA+AGG	+	Chr8:74990908-74990927	None:intergenic	40.0%
	GTCCAAGATGTTTCTTGAGT+AGG	+	Chr8:74989588-74989607	None:intergenic	40.0%
	TAGGTGCATATGCATCATCA+AGG	-	Chr8:74990476-74990495	MsG0880046569.01.T01:CDS	40.0%
	TCATCTATATCAGCATGGCT+TGG	+	Chr8:74990777-74990796	None:intergenic	40.0%
	TCCTCTCATCTATATCAGCA+TGG	+	Chr8:74990782-74990801	None:intergenic	40.0%
	TCTCCGTTTGAATATGCACT+TGG	-	Chr8:74991790-74991809	MsG0880046569.01.T01:intron	40.0%
	TGAAGAGCTTCTGGATATGA+AGG	+	Chr8:74991883-74991902	None:intergenic	40.0%
	TGACACAGCACAAAAATGTG+TGG	-	Chr8:74990371-74990390	MsG0880046569.01.T01:CDS	40.0%
	TGCATAGTGATCCAATAGCA+TGG	+	Chr8:74991420-74991439	None:intergenic	40.0%
	TGTCTCTTATCTGCAGATGA+TGG	-	Chr8:74989322-74989341	MsG0880046569.01.T01:intron	40.0%
	TTCCTACATAGCAAGACAAG+TGG	+	Chr8:74990700-74990719	None:intergenic	40.0%
	TTGACATCATTGTGAGCGTA+AGG	+	Chr8:74990135-74990154	None:intergenic	40.0%
	TTGATCCTGCAAGTTAGCAA+TGG	-	Chr8:74990978-74990997	MsG0880046569.01.T01:intron	40.0%
	TTTACTGATGCCCATGCTAT+TGG	-	Chr8:74991406-74991425	MsG0880046569.01.T01:intron	40.0%
!	CGTGTTTTCTTGACTCTTGA+GGG	-	Chr8:74991529-74991548	MsG0880046569.01.T01:intron	40.0%
!	CTTGTCTTGCTATGTAGGAA+TGG	-	Chr8:74990700-74990719	MsG0880046569.01.T01:intron	40.0%
!	GTAGAATGTGTCCCTTTTGT+TGG	-	Chr8:74989559-74989578	MsG0880046569.01.T01:intron	40.0%
!	GTGCCTTCATAACCACATTT+TGG	-	Chr8:74990902-74990921	MsG0880046569.01.T01:intron	40.0%
!	GTGTGTGTTTGATTTCACAG+TGG	+	Chr8:74989281-74989300	None:intergenic	40.0%
!	TCAATCCATTGCTAACTTGC+AGG	+	Chr8:74990986-74991005	None:intergenic	40.0%
!	TTCTTTTCGGTCATCTAGCT+TGG	-	Chr8:74990654-74990673	MsG0880046569.01.T01:intron	40.0%
!!	AAAATGGGTTGCTCATTCTC+CGG	-	Chr8:74988114-74988133	MsG0880046569.01.T01:three_prime_UTR	40.0%
!!	TGTGTGTATGTTTGGTTCTG+CGG	+	Chr8:74989237-74989256	None:intergenic	40.0%
!!!	GCTGTTGAGTTGATTTTACG+AGG	+	Chr8:74990074-74990093	None:intergenic	40.0%
	AAAGACTCTTCTCATCTCGC+AGG	-	Chr8:74988299-74988318	MsG0880046569.01.T01:CDS	45.0%
	AAATCCATCAGTATGGCCAG+TGG	+	Chr8:74990198-74990217	None:intergenic	45.0%
	AAGACCTGATTTCACGTCGT+CGG	+	Chr8:74988002-74988021	None:intergenic	45.0%
	ACAGAAACCAGTTCTGCAAG+TGG	+	Chr8:74990352-74990371	None:intergenic	45.0%
	ACATCATTGTGAGCGTAAGG+AGG	+	Chr8:74990132-74990151	None:intergenic	45.0%
	ACGAACTGATGAAGAGCTTC+TGG	+	Chr8:74991892-74991911	None:intergenic	45.0%
	AGAACTTCCACTTGCAGAAC+TGG	-	Chr8:74990342-74990361	MsG0880046569.01.T01:intron	45.0%
	AGGAGTTGAGGACCAAAATG+TGG	+	Chr8:74990917-74990936	None:intergenic	45.0%
	ATCAAGAAGTGGGAGGAGAT+TGG	+	Chr8:74989440-74989459	None:intergenic	45.0%
	ATGGCACATATGAGGAGTTG+AGG	+	Chr8:74990929-74990948	None:intergenic	45.0%
	CTCTTCATCAGTTCGTGTCA+TGG	-	Chr8:74991896-74991915	MsG0880046569.01.T01:CDS	45.0%
	GAATATGCACTTGGAGAGTC+TGG	-	Chr8:74991799-74991818	MsG0880046569.01.T01:intron	45.0%
	GACTAAACAGTGAAGAGACG+CGG	+	Chr8:74989414-74989433	None:intergenic	45.0%
	GAGCTAATCTTCTTCCTTGC+GGG	+	Chr8:74990234-74990253	None:intergenic	45.0%
	GCATATGCACCTACCACATT+AGG	+	Chr8:74990469-74990488	None:intergenic	45.0%
	GCCATGCTGATATAGATGAG+AGG	-	Chr8:74990778-74990797	MsG0880046569.01.T01:CDS	45.0%
	GCTCACAATGATGTCAAACC+TGG	-	Chr8:74990138-74990157	MsG0880046569.01.T01:intron	45.0%
	GGAGCTAATCTTCTTCCTTG+CGG	+	Chr8:74990235-74990254	None:intergenic	45.0%
	GGTGGAATAGTGTTCCTTCT+TGG	+	Chr8:74989682-74989701	None:intergenic	45.0%
	TACACCTTTCCTGCTTAGCT+CGG	-	Chr8:74989511-74989530	MsG0880046569.01.T01:intron	45.0%
	TAGATGAGAGGACTGACGTT+TGG	-	Chr8:74990790-74990809	MsG0880046569.01.T01:CDS	45.0%
	TAGTCTAGATAGTAGGGCGT+TGG	-	Chr8:74992165-74992184	MsG0880046569.01.T01:five_prime_UTR	45.0%
	TATCATCGTAGGGAACAGTG+TGG	-	Chr8:74992008-74992027	MsG0880046569.01.T01:CDS	45.0%
	TATGATTCTGTGAACGGAGG+TGG	-	Chr8:74988150-74988169	MsG0880046569.01.T01:three_prime_UTR	45.0%
	TCAAGAAGTGGGAGGAGATT+GGG	+	Chr8:74989439-74989458	None:intergenic	45.0%
	TCAAGTGCATGGCACATATG+AGG	+	Chr8:74990937-74990956	None:intergenic	45.0%
	TGAGTAGGCTGCCAACAAAA+GGG	+	Chr8:74989573-74989592	None:intergenic	45.0%
	TGTTCACATGTCTGTGTGTG+AGG	-	Chr8:74991087-74991106	MsG0880046569.01.T01:intron	45.0%
	TTGAGTAGGCTGCCAACAAA+AGG	+	Chr8:74989574-74989593	None:intergenic	45.0%
	TTGCATGATCAAGAAGTGGG+AGG	+	Chr8:74989447-74989466	None:intergenic	45.0%
!	AGCAGGAAAGGTGTAAGTGT+TGG	+	Chr8:74989506-74989525	None:intergenic	45.0%
!	AGTCTAGATAGTAGGGCGTT+GGG	-	Chr8:74992166-74992185	MsG0880046569.01.T01:five_prime_UTR	45.0%
!	CCGTGTTTTCTTGACTCTTG+AGG	-	Chr8:74991528-74991547	MsG0880046569.01.T01:intron	45.0%
!	CTAGCTTGGATTTTCTGACC+TGG	-	Chr8:74990668-74990687	MsG0880046569.01.T01:intron	45.0%
!	GAAGATTAGCTCCAGATCAG+AGG	-	Chr8:74990242-74990261	MsG0880046569.01.T01:intron	45.0%
!	GATGGCAATGTGTTATTCCG+TGG	-	Chr8:74988731-74988750	MsG0880046569.01.T01:intron	45.0%
!	TCAACTCAACAGCTTTGTGC+AGG	-	Chr8:74990081-74990100	MsG0880046569.01.T01:CDS	45.0%
!	TGCCACTTGTCTTGCTATGT+AGG	-	Chr8:74990695-74990714	MsG0880046569.01.T01:intron	45.0%
!!	AGTGACTCTGGCTCTTTAGT+AGG	+	Chr8:74991304-74991323	None:intergenic	45.0%
!!	TCTGGCTCTTTAGTAGGACT+TGG	+	Chr8:74991298-74991317	None:intergenic	45.0%
!!!	CTGGCCATACTGATGGATTT+TGG	-	Chr8:74990198-74990217	MsG0880046569.01.T01:intron	45.0%
	AGGACTGACGTTTGGTCACT+AGG	-	Chr8:74990798-74990817	MsG0880046569.01.T01:CDS	50.0%
	CAAGAAGTGGGAGGAGATTG+GGG	+	Chr8:74989438-74989457	None:intergenic	50.0%
	CAAGACAAGTGGCAAACACC+AGG	+	Chr8:74990689-74990708	None:intergenic	50.0%
	CACACACACACAGAGATCCA+CGG	+	Chr8:74988751-74988770	None:intergenic	50.0%
	CAGAAAAGGACAACCACCAC+TGG	-	Chr8:74990179-74990198	MsG0880046569.01.T01:intron	50.0%
	CATTCAGAACAACGAGCAGC+TGG	-	Chr8:74989369-74989388	MsG0880046569.01.T01:intron	50.0%
	GAGCTTATTAGCGAGACCAC+CGG	+	Chr8:74988279-74988298	None:intergenic	50.0%
	GCTCCTAATGACTCTGCTAC+CGG	-	Chr8:74988257-74988276	MsG0880046569.01.T01:CDS	50.0%
	TATGCACTTGGAGAGTCTGG+TGG	-	Chr8:74991802-74991821	MsG0880046569.01.T01:intron	50.0%
	TCAACCGACGACGTGAAATC+AGG	-	Chr8:74987995-74988014	MsG0880046569.01.T01:three_prime_UTR	50.0%
	TGAGGAAGACACAGGGAGAT+TGG	+	Chr8:74992252-74992271	None:intergenic	50.0%
!	AAAAGCATGCCGAGCTAAGC+AGG	+	Chr8:74989523-74989542	None:intergenic	50.0%
!	AGAATCGATTCCGCATCCTG+AGG	-	Chr8:74988190-74988209	MsG0880046569.01.T01:exon	50.0%
!	AGACTCGGTAAAGCTCGTGT+TGG	-	Chr8:74988541-74988560	MsG0880046569.01.T01:intron	50.0%
!	GCTGCTCGTTGTTCTGAATG+AGG	+	Chr8:74989369-74989388	None:intergenic	50.0%
!	TGAACGGAGGTGGTGATGTT+TGG	-	Chr8:74988160-74988179	MsG0880046569.01.T01:three_prime_UTR	50.0%
	ACCACCACTGGCCATACTGA+TGG	-	Chr8:74990191-74990210	MsG0880046569.01.T01:intron	55.0%
	ACTCTTGAGGGCTTCTCCCT+TGG	-	Chr8:74991541-74991560	MsG0880046569.01.T01:intron	55.0%
	ATGTCAAGAGGGCTGAGCTC+TGG	-	Chr8:74991258-74991277	MsG0880046569.01.T01:intron	55.0%
	CATGCCGAGCTAAGCAGGAA+AGG	+	Chr8:74989518-74989537	None:intergenic	55.0%
	CCACCGGTAGCAGAGTCATT+AGG	+	Chr8:74988263-74988282	None:intergenic	55.0%
	CCTAATGACTCTGCTACCGG+TGG	-	Chr8:74988260-74988279	MsG0880046569.01.T01:CDS	55.0%
	CGGTACGAGAGCTCCTTTCT+TGG	+	Chr8:74987919-74987938	None:intergenic	55.0%
	GAACAACGAGCAGCTGGAGT+TGG	-	Chr8:74989375-74989394	MsG0880046569.01.T01:CDS	55.0%
	TCCATCAGTATGGCCAGTGG+TGG	+	Chr8:74990195-74990214	None:intergenic	55.0%
	TCGTAGGGAACAGTGTGGCA+TGG	-	Chr8:74992013-74992032	MsG0880046569.01.T01:CDS	55.0%
	TTCAGCCCCAAGCTGCTATG+AGG	-	Chr8:74991923-74991942	MsG0880046569.01.T01:CDS	55.0%
	TTGGGCAATCCCACAGCTCA+GGG	+	Chr8:74990758-74990777	None:intergenic	55.0%
!	CAACTGGAGTGCCTCTGATC+TGG	+	Chr8:74990256-74990275	None:intergenic	55.0%
!!	ATCAGAGGCACTCCAGTTGC+AGG	-	Chr8:74990257-74990276	MsG0880046569.01.T01:intron	55.0%
	AATCCCACAGCTCAGGGGCT+CGG	+	Chr8:74990752-74990771	None:intergenic	60.0%
	ATCCTGAGGCAGCTCGGAGA+AGG	-	Chr8:74988204-74988223	MsG0880046569.01.T01:exon	60.0%
	CTTGGGCAATCCCACAGCTC+AGG	+	Chr8:74990759-74990778	None:intergenic	60.0%
	GCTCAGGTCAAATGGCCAGC+TGG	-	Chr8:74991850-74991869	MsG0880046569.01.T01:intron	60.0%
	GTCTGGTGGAAGCCTGCAAC+TGG	-	Chr8:74991816-74991835	MsG0880046569.01.T01:intron	60.0%
	TCATCAGGGGAGGGCGACTA+GGG	-	Chr8:74989780-74989799	MsG0880046569.01.T01:intron	60.0%
	TCCTGAGGCAGCTCGGAGAA+GGG	-	Chr8:74988205-74988224	MsG0880046569.01.T01:exon	60.0%
	TGGGCAATCCCACAGCTCAG+GGG	+	Chr8:74990757-74990776	None:intergenic	60.0%
	TTCATCAGGGGAGGGCGACT+AGG	-	Chr8:74989779-74989798	MsG0880046569.01.T01:intron	60.0%
	TTCCGCATCCTGAGGCAGCT+CGG	-	Chr8:74988198-74988217	MsG0880046569.01.T01:exon	60.0%
	TTTCCGAGCCCCTGAGCTGT+GGG	-	Chr8:74990746-74990765	MsG0880046569.01.T01:CDS	60.0%
!	ACGAGCAGCTGGAGTTGGTC+AGG	-	Chr8:74989380-74989399	MsG0880046569.01.T01:CDS	60.0%
!	CGAGCAGCTGGAGTTGGTCA+GGG	-	Chr8:74989381-74989400	MsG0880046569.01.T01:CDS	60.0%
!	GTGTGCGCGCGCGATTGTTT+TGG	-	Chr8:74988766-74988785	MsG0880046569.01.T01:intron	60.0%
!!	AAGAGGGCTGAGCTCTGGAG+AGG	-	Chr8:74991263-74991282	MsG0880046569.01.T01:intron	60.0%
!!	AGAGGGCTGAGCTCTGGAGA+GGG	-	Chr8:74991264-74991283	MsG0880046569.01.T01:intron	60.0%
	CACAGCTCAGGGGCTCGGAA+AGG	+	Chr8:74990747-74990766	None:intergenic	65.0%
	CCCAAGCTGCTATGAGGCCC+CGG	-	Chr8:74991929-74991948	MsG0880046569.01.T01:CDS	65.0%
	CCGGGGCCTCATAGCAGCTT+GGG	+	Chr8:74991932-74991951	None:intergenic	65.0%
	CCTGAGGCAGCTCGGAGAAG+GGG	-	Chr8:74988206-74988225	MsG0880046569.01.T01:exon	65.0%
	CGGGGCCTCATAGCAGCTTG+GGG	+	Chr8:74991931-74991950	None:intergenic	65.0%
	CTCCGAGCTGCCTCAGGATG+CGG	+	Chr8:74988203-74988222	None:intergenic	65.0%
	CTTTCCGAGCCCCTGAGCTG+TGG	-	Chr8:74990745-74990764	MsG0880046569.01.T01:CDS	65.0%
	TCCGGGGCCTCATAGCAGCT+TGG	+	Chr8:74991933-74991952	None:intergenic	65.0%
	TTGGCAGTGCTCGTCCCGCA+AGG	-	Chr8:74990217-74990236	MsG0880046569.01.T01:intron	65.0%
!	GCAGCTGGAGTTGGTCAGGG+AGG	-	Chr8:74989384-74989403	MsG0880046569.01.T01:CDS	65.0%
!!	GAGGGCTGAGCTCTGGAGAG+GGG	-	Chr8:74991265-74991284	MsG0880046569.01.T01:intron	65.0%
	CCCCTTCTCCGAGCTGCCTC+AGG	+	Chr8:74988209-74988228	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	74987880	74992332	74987880	ID=MsG0880046569.01;Name=MsG0880046569.01
Chr8	mRNA	74987880	74992332	74987880	ID=MsG0880046569.01.T01;Parent=MsG0880046569.01;Name=MsG0880046569.01.T01;_AED=0.36;_eAED=0.39;_QI=237\|0.42\|0.5\|1\|0.28\|0.5\|8\|327\|397
Chr8	exon	74987880	74988432	74987880	ID=MsG0880046569.01.T01:exon:31433;Parent=MsG0880046569.01.T01
Chr8	exon	74989371	74989495	74989371	ID=MsG0880046569.01.T01:exon:31432;Parent=MsG0880046569.01.T01
Chr8	exon	74989934	74990151	74989934	ID=MsG0880046569.01.T01:exon:31431;Parent=MsG0880046569.01.T01
Chr8	exon	74990351	74990411	74990351	ID=MsG0880046569.01.T01:exon:31430;Parent=MsG0880046569.01.T01
Chr8	exon	74990472	74990627	74990472	ID=MsG0880046569.01.T01:exon:31429;Parent=MsG0880046569.01.T01
Chr8	exon	74990732	74990874	74990732	ID=MsG0880046569.01.T01:exon:31428;Parent=MsG0880046569.01.T01
Chr8	exon	74991691	74991751	74991691	ID=MsG0880046569.01.T01:exon:31427;Parent=MsG0880046569.01.T01
Chr8	exon	74991892	74992332	74991892	ID=MsG0880046569.01.T01:exon:31426;Parent=MsG0880046569.01.T01
Chr8	five_prime_UTR	74992096	74992332	74992096	ID=MsG0880046569.01.T01:five_prime_utr;Parent=MsG0880046569.01.T01
Chr8	CDS	74991892	74992095	74991892	ID=MsG0880046569.01.T01:cds;Parent=MsG0880046569.01.T01
Chr8	CDS	74991691	74991751	74991691	ID=MsG0880046569.01.T01:cds;Parent=MsG0880046569.01.T01
Chr8	CDS	74990732	74990874	74990732	ID=MsG0880046569.01.T01:cds;Parent=MsG0880046569.01.T01
Chr8	CDS	74990472	74990627	74990472	ID=MsG0880046569.01.T01:cds;Parent=MsG0880046569.01.T01
Chr8	CDS	74990351	74990411	74990351	ID=MsG0880046569.01.T01:cds;Parent=MsG0880046569.01.T01
Chr8	CDS	74989934	74990151	74989934	ID=MsG0880046569.01.T01:cds;Parent=MsG0880046569.01.T01
Chr8	CDS	74989371	74989495	74989371	ID=MsG0880046569.01.T01:cds;Parent=MsG0880046569.01.T01
Chr8	CDS	74988207	74988432	74988207	ID=MsG0880046569.01.T01:cds;Parent=MsG0880046569.01.T01
Chr8	three_prime_UTR	74987880	74988206	74987880	ID=MsG0880046569.01.T01:three_prime_utr;Parent=MsG0880046569.01.T01

Gene Sequence

>MsG0880046569.01.T01

ATGGGTTGCTCATTCTCCGGTTTAAATGCATTATATGATTCTGTGAACGGAGGTGGTGATGTTTGGATCAACGAGAATCGATTCCGCATCCTGAGGCAGCTCGGAGAAGGGGCTTTGCTTACGTTTATCTCGTTAAGGAAGCTCCTAATGACTCTGCTACCGGTGGTCTCGCTAATAAGCTCAAAGACTCTTCTCATCTCGCAGCTGAATTTTCATTTGTGTTTCTGTTGTGTTGCATTGCATGCTCTTGTTATTGCGTTTTATGATGATGGATCTTATGCTATGAAAAAGGTCCTCATTCAGAACAACGAGCAGCTGGAGTTGGTCAGGGAGGAGATCCGCGTCTCTTCACTGTTTAGTCACCCCAATCTCCTCCCACTTCTTGATCATGCAATCATATCTGTTAAGCCTACTCAAGAAACATCTTGGACCCATGAAGCGTACTTGTTATTTCCAGTTCATTTGGATGGAACATTGCTAGACAATGCCAAAACAATGAAAGCCAAGAAGGAACACTATTCCACCTCAGATGTTCTTCAAATATTTCGGCAGGTAAAAAGGACAGGACCTCAAGCATCACTTTTGCAATTCAACATTTATTATCTGCCGTTTAGACCTTATTATAACCCTTTTAACCTCGTAAAATCAACTCAACAGCTTTGTGCAGGACTAAAGCACATGCACAATTTAGATCCTCCTTACGCTCACAATGATGTCAAACCTGGTAATGTTCTCATAACACACAGAAAAGGACAACCACCACTGGCCATACTGATGGATTTTGGCAGTGCTCGTCCCGCAAGGAAGAAGATTAGCTCCAGATCAGAGGCACTCCAGTTGCAGGAATGGGCATCTGAACATGTCTCTGCTCCTTTCCGAGCCCCTGAGCTGTGGGATTGCCCAAGCCATGCTGATATAGATGAGAGGACTGACGTTTGGTCACTAGGATGCACATTATATGCAATAATGTATGGGGTATCTCCGTTTGAATATGCACTTGGAGAGTCTGGTGGAAGCCTGCAACTGGCTATTGTAAATGCTCAGGTCAAATGGCCAGCTGGACTTAAACCTTCATATCCAGAAGCTCTTCATCAGTTCGTGTCATGGATGCTTCAGCCCCAAGCTGCTATGAGGCCCCGGATAGACGATATCATTATCCATGTTGATAAGTTGATTGCAAAGTTCTCTCAATGA

Protein sequence

>MsG0880046569.01.T01

MGCSFSGLNALYDSVNGGGDVWINENRFRILRQLGEGALLTFISLRKLLMTLLPVVSLISSKTLLISQLNFHLCFCCVALHALVIAFYDDGSYAMKKVLIQNNEQLELVREEIRVSSLFSHPNLLPLLDHAIISVKPTQETSWTHEAYLLFPVHLDGTLLDNAKTMKAKKEHYSTSDVLQIFRQVKRTGPQASLLQFNIYYLPFRPYYNPFNLVKSTQQLCAGLKHMHNLDPPYAHNDVKPGNVLITHRKGQPPLAILMDFGSARPARKKISSRSEALQLQEWASEHVSAPFRAPELWDCPSHADIDERTDVWSLGCTLYAIMYGVSPFEYALGESGGSLQLAIVNAQVKWPAGLKPSYPEALHQFVSWMLQPQAAMRPRIDDIIIHVDKLIAKFSQ*