AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380012403.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380012403.01.T01 MTR_1g010210 90.323 124 11 1 4 127 271 393 7.01e-71 219
MsG0380012403.01.T01 MTR_1g010270 79.839 124 24 1 4 127 255 377 1.21e-62 197
MsG0380012403.01.T01 MTR_3g108190 74.359 117 30 0 4 120 251 367 1.78e-57 184
MsG0380012403.01.T01 MTR_3g464210 80.180 111 21 1 1 110 271 381 3.73e-57 184
MsG0380012403.01.T01 MTR_4g011600 57.025 121 51 1 2 121 255 375 8.72e-43 146
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380012403.01.T01 AT2G25680 68.644 118 37 0 4 121 247 364 3.83e-55 178
MsG0380012403.01.T01 AT1G80310 55.372 121 52 2 2 121 242 361 2.30e-42 145

Find 28 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTAAACAAAATTGGATAAA+AGG 0.182185 3:+16032525 MsG0380012403.01.T01:CDS
GTAAATGAAAATTCCCTTTC+AGG 0.189818 3:-16032633 None:intergenic
TGAAATTCACTAAACAAAAT+TGG 0.301984 3:+16032517 MsG0380012403.01.T01:CDS
GGAGGGTGTGTGGCACTTCT+TGG 0.303917 3:+16032771 MsG0380012403.01.T01:CDS
TAATGAATTTAGTTGGTTCT+TGG 0.325172 3:+16032679 MsG0380012403.01.T01:CDS
CTAGCAGGACAATATAAATT+TGG 0.328674 3:+16032738 MsG0380012403.01.T01:CDS
GCAGGGTTTAGGAACATCTT+AGG 0.336845 3:+16032818 MsG0380012403.01.T01:CDS
AAAACTGGTATTGGGATTGC+AGG 0.365091 3:+16032800 MsG0380012403.01.T01:CDS
GTATTGGGATTGCAGGGTTT+AGG 0.376340 3:+16032807 MsG0380012403.01.T01:CDS
AATTTAGTTGGTTCTTGGTT+TGG 0.385997 3:+16032684 MsG0380012403.01.T01:CDS
TTCATTACTTCTATTGAAGA+TGG 0.415265 3:-16032498 None:intergenic
GTTGTAAACAAAATTAAAGT+TGG 0.432914 3:+16032474 MsG0380012403.01.T01:CDS
TAAATGAAAATTCCCTTTCA+GGG 0.439153 3:-16032632 None:intergenic
GTTGGACTAATGAATTTAGT+TGG 0.454255 3:+16032672 MsG0380012403.01.T01:CDS
AACATTGACAATGGAAGTTG+TGG 0.498635 3:-16032567 None:intergenic
GGTGCTGTGCCAACTTGTCA+TGG 0.504890 3:+16032705 MsG0380012403.01.T01:CDS
TATAAATTTGGTGAAAGAAG+TGG 0.504991 3:+16032750 MsG0380012403.01.T01:CDS
CATGGTGTTGGTGGACTAGC+AGG 0.518289 3:+16032723 MsG0380012403.01.T01:CDS
CCAACTTGTCATGGTGTTGG+TGG 0.525816 3:+16032714 MsG0380012403.01.T01:CDS
ACATCAATTTCAGTGACAGT+TGG 0.531284 3:+16032654 MsG0380012403.01.T01:CDS
GTGCCAACTTGTCATGGTGT+TGG 0.538904 3:+16032711 MsG0380012403.01.T01:CDS
TGAGAAATGTATATAAGAAG+TGG 0.542304 3:+16032450 None:intergenic
CCACCAACACCATGACAAGT+TGG 0.570149 3:-16032714 None:intergenic
AAACTGGTATTGGGATTGCA+GGG 0.575773 3:+16032801 MsG0380012403.01.T01:CDS
AAATTTGGTGAAAGAAGTGG+AGG 0.620983 3:+16032753 MsG0380012403.01.T01:CDS
ACTGAGTTCAACATTGACAA+TGG 0.640308 3:-16032576 None:intergenic
TGAAAGAAGTGGAGGGTGTG+TGG 0.671256 3:+16032761 MsG0380012403.01.T01:CDS
AATTTGGTGAAAGAAGTGGA+GGG 0.715450 3:+16032754 MsG0380012403.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTAAACAAAATTGGATAAA+AGG + Chr3:16032525-16032544 MsG0380012403.01.T01:CDS 20.0%
!! GTTGTAAACAAAATTAAAGT+TGG + Chr3:16032474-16032493 MsG0380012403.01.T01:CDS 20.0%
!! TGAAATTCACTAAACAAAAT+TGG + Chr3:16032517-16032536 MsG0380012403.01.T01:CDS 20.0%
!!! TGGATAAAAGGTTTTATTAA+AGG + Chr3:16032537-16032556 MsG0380012403.01.T01:CDS 20.0%
! TAAATGAAAATTCCCTTTCA+GGG - Chr3:16032635-16032654 None:intergenic 25.0%
! TTCATTACTTCTATTGAAGA+TGG - Chr3:16032501-16032520 None:intergenic 25.0%
!! TAATGAATTTAGTTGGTTCT+TGG + Chr3:16032679-16032698 MsG0380012403.01.T01:CDS 25.0%
!! TATAAATTTGGTGAAAGAAG+TGG + Chr3:16032750-16032769 MsG0380012403.01.T01:CDS 25.0%
CTAGCAGGACAATATAAATT+TGG + Chr3:16032738-16032757 MsG0380012403.01.T01:CDS 30.0%
GTAAATGAAAATTCCCTTTC+AGG - Chr3:16032636-16032655 None:intergenic 30.0%
GTTGGACTAATGAATTTAGT+TGG + Chr3:16032672-16032691 MsG0380012403.01.T01:CDS 30.0%
! AATTTAGTTGGTTCTTGGTT+TGG + Chr3:16032684-16032703 MsG0380012403.01.T01:CDS 30.0%
AACATTGACAATGGAAGTTG+TGG - Chr3:16032570-16032589 None:intergenic 35.0%
ACATCAATTTCAGTGACAGT+TGG + Chr3:16032654-16032673 MsG0380012403.01.T01:CDS 35.0%
ACTGAGTTCAACATTGACAA+TGG - Chr3:16032579-16032598 None:intergenic 35.0%
! AAATTTGGTGAAAGAAGTGG+AGG + Chr3:16032753-16032772 MsG0380012403.01.T01:CDS 35.0%
! AACAGATCTTTTCCCTGAAA+GGG + Chr3:16032620-16032639 MsG0380012403.01.T01:CDS 35.0%
! AATTTGGTGAAAGAAGTGGA+GGG + Chr3:16032754-16032773 MsG0380012403.01.T01:CDS 35.0%
! GGTTTTGCAAAACTGGTATT+GGG + Chr3:16032792-16032811 MsG0380012403.01.T01:CDS 35.0%
!! TGGTTTTGCAAAACTGGTAT+TGG + Chr3:16032791-16032810 MsG0380012403.01.T01:CDS 35.0%
!!! ACTTCTTGGTTTTGCAAAAC+TGG + Chr3:16032785-16032804 MsG0380012403.01.T01:CDS 35.0%
! AAAACTGGTATTGGGATTGC+AGG + Chr3:16032800-16032819 MsG0380012403.01.T01:CDS 40.0%
! AAACTGGTATTGGGATTGCA+GGG + Chr3:16032801-16032820 MsG0380012403.01.T01:CDS 40.0%
! CAACAGATCTTTTCCCTGAA+AGG + Chr3:16032619-16032638 MsG0380012403.01.T01:CDS 40.0%
GTATTGGGATTGCAGGGTTT+AGG + Chr3:16032807-16032826 MsG0380012403.01.T01:CDS 45.0%
CCACCAACACCATGACAAGT+TGG - Chr3:16032717-16032736 None:intergenic 50.0%
GTGCCAACTTGTCATGGTGT+TGG + Chr3:16032711-16032730 MsG0380012403.01.T01:CDS 50.0%
! CCAACTTGTCATGGTGTTGG+TGG + Chr3:16032714-16032733 MsG0380012403.01.T01:CDS 50.0%
! TGAAAGAAGTGGAGGGTGTG+TGG + Chr3:16032761-16032780 MsG0380012403.01.T01:CDS 50.0%
! GGTGCTGTGCCAACTTGTCA+TGG + Chr3:16032705-16032724 MsG0380012403.01.T01:CDS 55.0%
!! CATGGTGTTGGTGGACTAGC+AGG + Chr3:16032723-16032742 MsG0380012403.01.T01:CDS 55.0%
! GGAGGGTGTGTGGCACTTCT+TGG + Chr3:16032771-16032790 MsG0380012403.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 16032456 16032839 16032456 ID=MsG0380012403.01;Name=MsG0380012403.01
Chr3 mRNA 16032456 16032839 16032456 ID=MsG0380012403.01.T01;Parent=MsG0380012403.01;Name=MsG0380012403.01.T01;_AED=0.51;_eAED=0.51;_QI=0|-1|0|1|-1|1|1|0|127
Chr3 exon 16032456 16032839 16032456 ID=MsG0380012403.01.T01:exon:21711;Parent=MsG0380012403.01.T01
Chr3 CDS 16032456 16032839 16032456 ID=MsG0380012403.01.T01:cds;Parent=MsG0380012403.01.T01
Gene Sequence

>MsG0380012403.01.T01

ATGTATATAAGAAGTGGAGTTGTAAACAAAATTAAAGTTGGACCATCTTCAATAGAAGTAATGAAATTCACTAAACAAAATTGGATAAAAGGTTTTATTAAAGGTGCAATTCCACAACTTCCATTGTCAATGTTGAACTCAGTGATAGCTGTTTGTAAATTGTCAACAGATCTTTTCCCTGAAAGGGAATTTTCATTTACATCAATTTCAGTGACAGTTGGACTAATGAATTTAGTTGGTTCTTGGTTTGGTGCTGTGCCAACTTGTCATGGTGTTGGTGGACTAGCAGGACAATATAAATTTGGTGAAAGAAGTGGAGGGTGTGTGGCACTTCTTGGTTTTGCAAAACTGGTATTGGGATTGCAGGGTTTAGGAACATCTTAG

Protein sequence

>MsG0380012403.01.T01

MYIRSGVVNKIKVGPSSIEVMKFTKQNWIKGFIKGAIPQLPLSMLNSVIAVCKLSTDLFPEREFSFTSISVTVGLMNLVGSWFGAVPTCHGVGGLAGQYKFGERSGGCVALLGFAKLVLGLQGLGTS*