AlfalfaGEDB | MsG0180003514.01

M. sativa cultivar ZhongmuNo.1 / MsG0180003514.01

KEGG: -
GO: GO:0009733
PFAM: PF02519

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003514.01.T01	XP_013468157.1	93.636	110	5	1	1	110	1	108	7.18E-68	209

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003514.01.T01	A0A072VJG8	93.636	110	5	1	1	110	1	108	3.43e-68	209

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048198.01	MsG0180003514.01	0.806673	7.337987e-50	4.006709e-47
MsG0080048273.01	MsG0180003514.01	0.813860	2.066491e-51	1.365027e-48
MsG0080048274.01	MsG0180003514.01	0.804056	2.593778e-49	1.324388e-46
MsG0180000843.01	MsG0180003514.01	0.800140	1.657561e-48	7.659903e-46
MsG0180003514.01	MsG0180003933.01	0.832774	7.800079e-56	8.806437e-53
MsG0180003514.01	MsG0180004792.01	0.805386	1.368645e-49	7.231111e-47
MsG0180003514.01	MsG0180005557.01	0.800567	1.356831e-48	6.338370e-46
MsG0180003514.01	MsG0180005898.01	0.805811	1.114497e-49	5.953240e-47
MsG0180003514.01	MsG0180006010.01	-0.811770	5.929741e-51	3.702786e-48
MsG0180003514.01	MsG0280006319.01	0.803323	3.682860e-49	1.845192e-46
MsG0180003514.01	MsG0280007511.01	0.802822	4.675187e-49	2.312788e-46
MsG0180003514.01	MsG0280008309.01	0.822808	1.943242e-53	1.643703e-50
MsG0180003514.01	MsG0280009445.01	0.836271	1.031657e-56	1.293787e-53
MsG0180003514.01	MsG0280010496.01	0.813259	2.802351e-51	1.821043e-48
MsG0180003514.01	MsG0380013177.01	0.823116	1.647109e-53	1.405448e-50
MsG0180003514.01	MsG0380014646.01	0.807213	5.639968e-50	3.123108e-47
MsG0180003514.01	MsG0380016354.01	0.812375	4.376211e-51	2.777731e-48
MsG0180003514.01	MsG0380016452.01	0.813094	3.045422e-51	1.970141e-48
MsG0180003514.01	MsG0380017532.01	0.832718	8.057953e-56	9.082318e-53
MsG0180003514.01	MsG0380017976.01	0.815595	8.528925e-52	5.905868e-49
MsG0180003514.01	MsG0480018629.01	0.841077	5.911391e-58	8.587609e-55
MsG0180003514.01	MsG0480018631.01	0.801052	1.079995e-48	5.107994e-46
MsG0180003514.01	MsG0480018905.01	0.817394	3.373344e-52	2.453768e-49
MsG0180003514.01	MsG0480019592.01	0.804805	1.810832e-49	9.426110e-47
MsG0180003514.01	MsG0480019974.01	0.823698	1.204196e-53	1.044679e-50
MsG0180003514.01	MsG0480020027.01	0.804633	1.967294e-49	1.019413e-46
MsG0180003514.01	MsG0480021999.01	0.800281	1.551411e-48	7.195215e-46
MsG0180003514.01	MsG0480022240.01	0.804806	1.809860e-49	9.421391e-47
MsG0180003514.01	MsG0480023466.01	0.804516	2.080352e-49	1.074755e-46
MsG0180003514.01	MsG0480023616.01	0.801586	8.396379e-49	4.024650e-46
MsG0180003514.01	MsG0580024386.01	0.818717	1.694221e-52	1.277977e-49
MsG0180003514.01	MsG0580024567.01	0.803332	3.666517e-49	1.837472e-46
MsG0180003514.01	MsG0580024764.01	0.813093	3.047871e-51	1.971641e-48
MsG0180003514.01	MsG0580024766.01	0.810896	9.176413e-51	5.599769e-48
MsG0180003514.01	MsG0580029700.01	0.829247	5.728593e-55	5.830869e-52
MsG0180003514.01	MsG0580029719.01	0.801136	1.038131e-48	4.920335e-46
MsG0180003514.01	MsG0580029732.01	0.810068	1.385088e-50	8.268673e-48
MsG0180003514.01	MsG0580030063.01	0.840121	1.052258e-57	1.484304e-54
MsG0180003514.01	MsG0680030776.01	0.820060	8.374046e-53	6.557357e-50
MsG0180003514.01	MsG0680033901.01	0.805668	1.194506e-49	6.357117e-47
MsG0180003514.01	MsG0680033902.01	0.800784	1.225324e-48	5.755461e-46
MsG0180003514.01	MsG0680034724.01	0.816996	4.146457e-52	2.983502e-49
MsG0180003514.01	MsG0680034730.01	0.822114	2.816698e-53	2.335790e-50
MsG0180003514.01	MsG0680035665.01	0.804916	1.716981e-49	8.963448e-47
MsG0180003514.01	MsG0780038653.01	0.809892	1.511771e-50	8.983010e-48
MsG0180003514.01	MsG0780038848.01	0.829306	5.542887e-55	5.652147e-52
MsG0180003514.01	MsG0780039805.01	0.836339	9.917431e-57	1.246223e-53
MsG0180003514.01	MsG0880044312.01	0.839058	1.988171e-57	2.713717e-54
MsG0180003514.01	MsG0880044435.01	0.817799	2.734656e-52	2.011362e-49
MsG0180003514.01	MsG0880044834.01	0.811567	6.561965e-51	4.075782e-48
MsG0180003514.01	MsG0880045073.01	0.801102	1.054885e-48	4.995560e-46
MsG0180003514.01	MsG0880046324.01	0.807591	4.690053e-50	2.622547e-47
MsG0180003514.01	MsG0880046759.01	0.804455	2.142709e-49	1.105247e-46
MsG0180003514.01	MsG0880047312.01	-0.811550	6.620979e-51	4.110518e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003514.01.T01	MTR_1g063260	93.636	110	5	1	1	110	1	108	8.70e-72	209
MsG0180003514.01.T01	MTR_1g063300	86.239	109	11	1	1	109	1	105	2.74e-65	192
MsG0180003514.01.T01	MTR_1g063280	87.037	108	13	1	1	108	1	107	1.24e-64	191
MsG0180003514.01.T01	MTR_1g063290	85.455	110	12	1	1	110	1	106	8.69e-64	189
MsG0180003514.01.T01	MTR_1g063330	55.046	109	38	1	1	109	1	98	2.60e-36	119
MsG0180003514.01.T01	MTR_1g063340	52.294	109	41	1	1	109	1	98	9.25e-35	115
MsG0180003514.01.T01	MTR_1g063310	55.140	107	31	1	1	107	1	90	8.47e-34	113
MsG0180003514.01.T01	MTR_7g101890	47.297	74	39	0	37	110	46	119	8.47e-23	85.9
MsG0180003514.01.T01	MTR_4g057810	50.725	69	34	0	40	108	35	103	5.52e-19	76.3
MsG0180003514.01.T01	MTR_2g095430	41.558	77	43	1	34	108	36	112	2.92e-17	72.0
MsG0180003514.01.T01	MTR_7g101920	44.776	67	37	0	43	109	35	101	4.31e-16	68.6
MsG0180003514.01.T01	MTR_3g092220	32.292	96	61	2	19	110	8	103	8.01e-15	65.5
MsG0180003514.01.T01	MTR_4g057800	46.377	69	35	1	39	105	34	102	2.90e-14	64.3
MsG0180003514.01.T01	MTR_8g026730	43.548	62	35	0	43	104	27	88	2.32e-13	61.2
MsG0180003514.01.T01	MTR_1g009150	44.444	72	38	1	34	105	62	131	1.97e-12	60.5
MsG0180003514.01.T01	MTR_7g118010	41.935	62	36	0	43	104	23	84	2.65e-12	58.5
MsG0180003514.01.T01	MTR_4g005320	45.455	66	34	1	40	105	67	130	7.03e-12	58.9
MsG0180003514.01.T01	MTR_1g061570	38.889	72	42	1	34	105	10	79	2.46e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003514.01.T01	AT3G09870	42.308	78	44	1	34	110	36	113	2.35e-17	72.0
MsG0180003514.01.T01	AT2G28085	47.222	72	36	1	39	108	51	122	5.55e-15	65.9
MsG0180003514.01.T01	AT4G00880	33.019	106	55	3	8	110	6	98	1.83e-12	59.7
MsG0180003514.01.T01	AT2G46690	39.726	73	40	2	42	110	23	95	7.63e-12	57.8
MsG0180003514.01.T01	AT3G61900	38.356	73	44	1	25	96	12	84	8.05e-12	58.2
MsG0180003514.01.T01	AT3G43120	42.254	71	39	1	27	97	63	131	1.13e-11	58.2

Find 19 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGCTTGATTCATTTGT+TGG	0.341379	1:+63525864	None:intergenic
GATGATGCTTGGGAGGAGTA+TGG	0.392995	1:+63526098	MsG0180003514.01.T01:CDS
CTTTGCTGTTCTTGCAAAGA+AGG	0.394733	1:+63526007	MsG0180003514.01.T01:CDS
TGGGATTGCTTGATGATGCT+TGG	0.395014	1:+63526087	MsG0180003514.01.T01:CDS
GGGATTGCTTGATGATGCTT+GGG	0.409014	1:+63526088	MsG0180003514.01.T01:CDS
GAAACTAGAAGGTTTATTGT+TGG	0.428359	1:+63526035	MsG0180003514.01.T01:CDS
AAATGCTTGATTCATTTGTT+GGG	0.431852	1:+63525865	None:intergenic
CTCACTTGTGGTTGTGATTG+TGG	0.437138	1:-63525958	None:intergenic
GGAGTATGGTTTCAGACAAA+AGG	0.443046	1:+63526112	MsG0180003514.01.T01:CDS
AGAAGGGTGTAGAAACTAGA+AGG	0.457872	1:+63526024	MsG0180003514.01.T01:CDS
GCTTGATTCATTTGTTGGGA+AGG	0.508242	1:+63525869	MsG0180003514.01.T01:CDS
GAACTCTCGTTGTTCCGTGC+CGG	0.527691	1:+63526135	MsG0180003514.01.T01:CDS
TACAAAACATTCTAGATAGT+CGG	0.531235	1:+63526168	MsG0180003514.01.T01:CDS
TTTGCTGTTCTTGCAAAGAA+GGG	0.553621	1:+63526008	MsG0180003514.01.T01:CDS
GAGTATGGTTTCAGACAAAA+GGG	0.556094	1:+63526113	MsG0180003514.01.T01:CDS
ATCATCAGCAAGCTCACTTG+TGG	0.599516	1:-63525970	None:intergenic
ATTGCTTGATGATGCTTGGG+AGG	0.606537	1:+63526091	MsG0180003514.01.T01:CDS
CTTGCTGATGATGTTATGAA+AGG	0.606752	1:+63525981	MsG0180003514.01.T01:CDS
TGTAAGTCGATCGGCCGGCA+CGG	0.614103	1:-63526149	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TACAAAACATTCTAGATAGT+CGG	+	Chr1:63526168-63526187	MsG0180003514.01.T01:CDS	25.0%
	GAAACTAGAAGGTTTATTGT+TGG	+	Chr1:63526035-63526054	MsG0180003514.01.T01:CDS	30.0%
!	AGAATGTTTTGTAAGTCGAT+CGG	-	Chr1:63526161-63526180	None:intergenic	30.0%
!!!	TAACTCAGTGCATGTTTTTT+TGG	-	Chr1:63525921-63525940	None:intergenic	30.0%
!!!	TTAACTGATCCTGCTTTTTT+GGG	+	Chr1:63526068-63526087	MsG0180003514.01.T01:CDS	30.0%
	AAAAACATGCACTGAGTTAC+AGG	+	Chr1:63525922-63525941	MsG0180003514.01.T01:CDS	35.0%
	GAGTATGGTTTCAGACAAAA+GGG	+	Chr1:63526113-63526132	MsG0180003514.01.T01:CDS	35.0%
!	CTTGCTGATGATGTTATGAA+AGG	+	Chr1:63525981-63526000	MsG0180003514.01.T01:CDS	35.0%
!!	TTTGCTGTTCTTGCAAAGAA+GGG	+	Chr1:63526008-63526027	MsG0180003514.01.T01:CDS	35.0%
!!!	CTTAACTGATCCTGCTTTTT+TGG	+	Chr1:63526067-63526086	MsG0180003514.01.T01:CDS	35.0%
	GGAGTATGGTTTCAGACAAA+AGG	+	Chr1:63526112-63526131	MsG0180003514.01.T01:CDS	40.0%
	TCAAGCAATCCCAAAAAAGC+AGG	-	Chr1:63526080-63526099	None:intergenic	40.0%
!	AGAAGGGTGTAGAAACTAGA+AGG	+	Chr1:63526024-63526043	MsG0180003514.01.T01:CDS	40.0%
!	GCTTGATTCATTTGTTGGGA+AGG	+	Chr1:63525869-63525888	MsG0180003514.01.T01:CDS	40.0%
!!	CTTTGCTGTTCTTGCAAAGA+AGG	+	Chr1:63526007-63526026	MsG0180003514.01.T01:CDS	40.0%
	ATCATCAGCAAGCTCACTTG+TGG	-	Chr1:63525973-63525992	None:intergenic	45.0%
	CTCACTTGTGGTTGTGATTG+TGG	-	Chr1:63525961-63525980	None:intergenic	45.0%
!	TGTTTTGTAAGTCGATCGGC+CGG	-	Chr1:63526157-63526176	None:intergenic	45.0%
!!	ATTGCTTGATGATGCTTGGG+AGG	+	Chr1:63526091-63526110	MsG0180003514.01.T01:CDS	45.0%
!!	GGGATTGCTTGATGATGCTT+GGG	+	Chr1:63526088-63526107	MsG0180003514.01.T01:CDS	45.0%
!!	TGGGATTGCTTGATGATGCT+TGG	+	Chr1:63526087-63526106	MsG0180003514.01.T01:CDS	45.0%
	GATGATGCTTGGGAGGAGTA+TGG	+	Chr1:63526098-63526117	MsG0180003514.01.T01:CDS	50.0%
	GAACTCTCGTTGTTCCGTGC+CGG	+	Chr1:63526135-63526154	MsG0180003514.01.T01:CDS	55.0%
	TGTAAGTCGATCGGCCGGCA+CGG	-	Chr1:63526152-63526171	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	63525867	63526199	63525867	ID=MsG0180003514.01;Name=MsG0180003514.01
Chr1	mRNA	63525867	63526199	63525867	ID=MsG0180003514.01.T01;Parent=MsG0180003514.01;Name=MsG0180003514.01.T01;_AED=0.46;_eAED=0.46;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|110
Chr1	exon	63525867	63526199	63525867	ID=MsG0180003514.01.T01:exon:4679;Parent=MsG0180003514.01.T01
Chr1	CDS	63525867	63526199	63525867	ID=MsG0180003514.01.T01:cds;Parent=MsG0180003514.01.T01

Gene Sequence	>MsG0180003514.01.T01 ATGCTTGATTCATTTGTTGGGAAGGTACAAAAGAGTTTATCACTCTTTGTACCAAAAAAACATGCACTGAGTTACAGGAATGAAGATCATGCCACAATCACAACCACAAGTGAGCTTGCTGATGATGTTATGAAAGGTTACTTTGCTGTTCTTGCAAAGAAGGGTGTAGAAACTAGAAGGTTTATTGTTGGATTAGATTACTTAACTGATCCTGCTTTTTTGGGATTGCTTGATGATGCTTGGGAGGAGTATGGTTTCAGACAAAAGGGAACTCTCGTTGTTCCGTGCCGGCCGATCGACTTACAAAACATTCTAGATAGTCGGAAAACATAG
Protein sequence	>MsG0180003514.01.T01 MLDSFVGKVQKSLSLFVPKKHALSYRNEDHATITTTSELADDVMKGYFAVLAKKGVETRRFIVGLDYLTDPAFLGLLDDAWEEYGFRQKGTLVVPCRPIDLQNILDSRKT*