AlfalfaGEDB | MsG0180005557.01

M. sativa cultivar ZhongmuNo.1 / MsG0180005557.01

KEGG: -
GO: -
PFAM: PF00504

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005557.01.T01	AFK46043.1	76.963	191	9	1	1	156	10	200	1.04E-92	277

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005557.01.T01	P11432	68.817	186	26	1	3	156	11	196	1.71E-84	249

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005557.01.T01	I3T0K1	76.963	191	9	1	1	156	10	200	4.97e-93	277

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048198.01	MsG0180005557.01	0.823988	1.029344e-53	9.001645e-51
MsG0080048273.01	MsG0180005557.01	0.811133	8.154724e-51	5.007389e-48
MsG0080048845.01	MsG0180005557.01	0.807063	6.069518e-50	3.347785e-47
MsG0080048912.01	MsG0180005557.01	0.830304	3.166685e-55	3.323283e-52
MsG0080049072.01	MsG0180005557.01	0.815011	1.150268e-51	7.838614e-49
MsG0080049129.01	MsG0180005557.01	0.848181	7.217072e-60	1.313365e-56
MsG0080049130.01	MsG0180005557.01	0.836791	7.606132e-57	9.688134e-54
MsG0180000017.01	MsG0180005557.01	0.837624	4.658131e-57	6.086111e-54
MsG0180000412.01	MsG0180005557.01	0.813192	2.898393e-51	1.880061e-48
MsG0180000489.01	MsG0180005557.01	0.806115	9.623349e-50	5.180457e-47
MsG0180000490.01	MsG0180005557.01	0.828804	7.335063e-55	7.368474e-52
MsG0180000491.01	MsG0180005557.01	0.827318	1.671170e-54	1.607903e-51
MsG0180000638.01	MsG0180005557.01	0.814033	1.892568e-51	1.256045e-48
MsG0180000843.01	MsG0180005557.01	0.850124	2.079548e-60	4.031683e-57
MsG0180001371.01	MsG0180005557.01	0.803908	2.784163e-49	1.416203e-46
MsG0180001772.01	MsG0180005557.01	0.805210	1.490171e-49	7.837735e-47
MsG0180001864.01	MsG0180005557.01	0.801920	7.170161e-49	3.466193e-46
MsG0180001929.01	MsG0180005557.01	0.801001	1.106135e-48	5.225009e-46
MsG0180003196.01	MsG0180005557.01	0.831849	1.321642e-55	1.451988e-52
MsG0180003316.01	MsG0180005557.01	0.827762	1.307857e-54	1.274510e-51
MsG0180003352.01	MsG0180005557.01	0.881489	2.300797e-70	1.350718e-66
MsG0180003514.01	MsG0180005557.01	0.800567	1.356831e-48	6.338370e-46
MsG0180003617.01	MsG0180005557.01	0.828914	6.895940e-55	6.949522e-52
MsG0180003748.01	MsG0180005557.01	0.804113	2.524908e-49	1.291028e-46
MsG0180003933.01	MsG0180005557.01	0.818551	1.847899e-52	1.387542e-49
MsG0180004662.01	MsG0180005557.01	0.890308	1.107697e-73	9.265143e-70
MsG0180004792.01	MsG0180005557.01	0.820960	5.202858e-53	4.177375e-50
MsG0180005184.01	MsG0180005557.01	0.840178	1.016742e-57	1.436736e-54
MsG0180005416.01	MsG0180005557.01	0.826747	2.289139e-54	2.165748e-51
MsG0180005545.01	MsG0180005557.01	0.965194	2.732776e-124	1.696636e-118
MsG0180005555.01	MsG0180005557.01	0.958816	9.219426e-117	3.523114e-111
MsG0180005556.01	MsG0180005557.01	0.950297	2.202592e-108	4.644486e-103
MsG0180005557.01	MsG0180005558.01	0.951459	1.952623e-109	4.463507e-104
MsG0180005557.01	MsG0180005561.01	0.804340	2.264485e-49	1.164630e-46
MsG0180005557.01	MsG0180005803.01	0.847378	1.201503e-59	2.131396e-56
MsG0180005557.01	MsG0180005854.01	0.815195	1.046925e-51	7.169687e-49
MsG0180005557.01	MsG0180005898.01	0.838647	2.539070e-57	3.422836e-54
MsG0180005557.01	MsG0180006176.01	0.803849	2.864129e-49	1.454633e-46
MsG0180005557.01	MsG0180006217.01	0.826872	2.137528e-54	2.029642e-51
MsG0180005557.01	MsG0280006319.01	0.864403	1.246504e-64	3.909122e-61
MsG0180005557.01	MsG0280006402.01	0.809346	1.979985e-50	1.159717e-47
MsG0180005557.01	MsG0280006571.01	0.811478	6.861849e-51	4.252159e-48
MsG0180005557.01	MsG0280007423.01	0.871020	9.391625e-67	3.730614e-63
MsG0180005557.01	MsG0280007511.01	0.843985	1.000502e-58	1.592480e-55
MsG0180005557.01	MsG0280007783.01	0.800778	1.228381e-48	5.769078e-46
MsG0180005557.01	MsG0280008227.01	0.800626	1.319603e-48	6.173782e-46
MsG0180005557.01	MsG0280008309.01	0.820171	7.898612e-53	6.204009e-50
MsG0180005557.01	MsG0280008419.01	0.823200	1.573914e-53	1.346129e-50
MsG0180005557.01	MsG0280009751.01	0.803568	3.276037e-49	1.651906e-46
MsG0180005557.01	MsG0280009863.01	0.861862	7.613802e-64	2.185112e-60
MsG0180005557.01	MsG0280010198.01	0.838684	2.484440e-57	3.352788e-54
MsG0180005557.01	MsG0280010496.01	0.832112	1.138493e-55	1.260195e-52
MsG0180005557.01	MsG0280010728.01	0.820583	6.351874e-53	5.045874e-50
MsG0180005557.01	MsG0280010926.01	0.825735	3.986278e-54	3.664356e-51
MsG0180005557.01	MsG0280011169.01	0.803050	4.193916e-49	2.086593e-46
MsG0180005557.01	MsG0280011218.01	0.805082	1.584675e-49	8.307543e-47
MsG0180005557.01	MsG0280011270.01	0.808295	3.323069e-50	1.892958e-47
MsG0180005557.01	MsG0280011362.01	0.804680	1.923030e-49	9.977461e-47
MsG0180005557.01	MsG0280011411.01	0.812276	4.598921e-51	2.911508e-48
MsG0180005557.01	MsG0380011632.01	0.842263	2.876507e-58	4.337647e-55
MsG0180005557.01	MsG0380012065.01	0.803719	3.047601e-49	1.542575e-46
MsG0180005557.01	MsG0380013177.01	0.808648	2.793692e-50	1.606067e-47
MsG0180005557.01	MsG0380013501.01	0.801990	6.936109e-49	3.359012e-46
MsG0180005557.01	MsG0380014144.01	0.811978	5.342770e-51	3.354906e-48
MsG0180005557.01	MsG0380014646.01	0.871638	5.869575e-67	2.385173e-63
MsG0180005557.01	MsG0380014933.01	0.809360	1.966600e-50	1.152315e-47
MsG0180005557.01	MsG0380015129.01	0.804321	2.285119e-49	1.174664e-46
MsG0180005557.01	MsG0380015496.01	0.832160	1.107857e-55	1.228031e-52
MsG0180005557.01	MsG0380015744.01	0.843573	1.289611e-58	2.026342e-55
MsG0180005557.01	MsG0380016066.01	0.863864	1.835987e-64	5.649216e-61
MsG0180005557.01	MsG0380016151.01	0.826495	2.629848e-54	2.470164e-51
MsG0180005557.01	MsG0380016167.01	0.815898	7.300048e-52	5.096600e-49
MsG0180005557.01	MsG0380016194.01	0.809801	1.581180e-50	9.373732e-48
MsG0180005557.01	MsG0380016284.01	0.825505	4.518551e-54	4.126349e-51
MsG0180005557.01	MsG0380016354.01	0.810573	1.077863e-50	6.520500e-48
MsG0180005557.01	MsG0380016459.01	0.810274	1.250687e-50	7.506229e-48
MsG0180005557.01	MsG0380016542.01	0.822081	2.866495e-53	2.374808e-50
MsG0180005557.01	MsG0380016545.01	0.810975	8.820710e-51	5.394415e-48
MsG0180005557.01	MsG0380016751.01	0.831950	1.248243e-55	1.375382e-52
MsG0180005557.01	MsG0380016762.01	0.842377	2.684305e-58	4.062458e-55
MsG0180005557.01	MsG0380016936.01	0.866969	1.932158e-65	6.635049e-62
MsG0180005557.01	MsG0380016997.01	0.873707	1.193839e-67	5.231098e-64
MsG0180005557.01	MsG0380017353.01	0.853169	2.854283e-61	6.111058e-58
MsG0180005557.01	MsG0380017532.01	0.888299	6.675352e-73	5.143125e-69
MsG0180005557.01	MsG0380017666.01	-0.836856	7.322592e-57	9.344683e-54
MsG0180005557.01	MsG0380017918.01	0.890395	1.023998e-73	8.595722e-70
MsG0180005557.01	MsG0480018232.01	0.806845	6.749745e-50	3.702152e-47
MsG0180005557.01	MsG0480018329.01	0.801753	7.759585e-49	3.735201e-46
MsG0180005557.01	MsG0480018629.01	0.824338	8.521406e-54	7.525825e-51
MsG0180005557.01	MsG0480018905.01	0.827668	1.378050e-54	1.339233e-51
MsG0180005557.01	MsG0480019573.01	0.818880	1.555910e-52	1.179001e-49
MsG0180005557.01	MsG0480019592.01	0.822057	2.904257e-53	2.404402e-50
MsG0180005557.01	MsG0480019974.01	0.823928	1.063470e-53	9.284707e-51
MsG0180005557.01	MsG0480020027.01	0.873334	1.593723e-67	6.890420e-64
MsG0180005557.01	MsG0480020473.01	0.816454	5.483641e-52	3.887164e-49
MsG0180005557.01	MsG0480020689.01	0.809873	1.525600e-50	9.061103e-48
MsG0180005557.01	MsG0480020704.01	0.864770	9.570523e-65	3.040457e-61
MsG0180005557.01	MsG0480021777.01	0.801199	1.007979e-48	4.784801e-46
MsG0180005557.01	MsG0480021817.01	0.835961	1.237280e-56	1.536848e-53
MsG0180005557.01	MsG0480021875.01	0.821660	3.587817e-53	2.937131e-50
MsG0180005557.01	MsG0480021999.01	0.826172	3.138224e-54	2.920935e-51
MsG0180005557.01	MsG0480022226.01	0.816692	4.851316e-52	3.461354e-49
MsG0180005557.01	MsG0480022617.01	0.813277	2.776460e-51	1.805212e-48
MsG0180005557.01	MsG0480022646.01	0.809393	1.934252e-50	1.134329e-47
MsG0180005557.01	MsG0480022664.01	0.806747	7.080502e-50	3.873584e-47
MsG0180005557.01	MsG0480022805.01	0.889818	1.722874e-73	1.411749e-69
MsG0180005557.01	MsG0480023395.01	0.845865	3.109912e-59	5.255292e-56
MsG0180005557.01	MsG0480023466.01	0.803065	4.164495e-49	2.072686e-46
MsG0180005557.01	MsG0480023505.01	0.829737	4.355007e-55	4.496856e-52
MsG0180005557.01	MsG0580024386.01	0.845592	3.690056e-59	6.179491e-56
MsG0180005557.01	MsG0580024471.01	0.892380	1.674699e-74	1.527172e-70
MsG0180005557.01	MsG0580024567.01	0.867946	9.400791e-66	3.341376e-62
MsG0180005557.01	MsG0580024679.01	0.803403	3.544324e-49	1.779549e-46
MsG0180005557.01	MsG0580024763.01	0.850891	1.266255e-60	2.517407e-57
MsG0180005557.01	MsG0580024764.01	0.870796	1.112701e-66	4.384425e-63
MsG0180005557.01	MsG0580024766.01	0.813500	2.480018e-51	1.622360e-48
MsG0180005557.01	MsG0580025097.01	0.813024	3.155711e-51	2.037721e-48
MsG0180005557.01	MsG0580025252.01	0.835935	1.255749e-56	1.558613e-53
MsG0180005557.01	MsG0580025413.01	0.837592	4.745946e-57	6.194466e-54
MsG0180005557.01	MsG0580027082.01	0.820047	8.431671e-53	6.600108e-50
MsG0180005557.01	MsG0580027098.01	0.814031	1.894515e-51	1.257276e-48
MsG0180005557.01	MsG0580027236.01	0.814422	1.552959e-51	1.041619e-48
MsG0180005557.01	MsG0580027254.01	0.822043	2.924943e-53	2.420613e-50
MsG0180005557.01	MsG0580027331.01	0.813523	2.451683e-51	1.604804e-48
MsG0180005557.01	MsG0580027458.01	0.800998	1.108032e-48	5.233527e-46
MsG0180005557.01	MsG0580027803.01	0.824661	7.150970e-54	6.375858e-51
MsG0180005557.01	MsG0580027899.01	0.819006	1.456885e-52	1.107883e-49
MsG0180005557.01	MsG0580028024.01	0.802579	5.247254e-49	2.579540e-46
MsG0180005557.01	MsG0580028111.01	0.841425	4.789631e-58	7.035230e-55
MsG0180005557.01	MsG0580028495.01	0.843536	1.318975e-58	2.070021e-55
MsG0180005557.01	MsG0580028925.01	0.857449	1.619877e-62	3.999611e-59
MsG0180005557.01	MsG0580029682.01	0.824560	7.554659e-54	6.715436e-51
MsG0180005557.01	MsG0580029700.01	0.802887	4.532743e-49	2.245928e-46
MsG0180005557.01	MsG0580029719.01	0.837868	4.031169e-57	5.306397e-54
MsG0180005557.01	MsG0580030063.01	0.855194	7.431422e-62	1.701623e-58
MsG0180005557.01	MsG0680030464.01	0.819154	1.348239e-52	1.029295e-49
MsG0180005557.01	MsG0680030776.01	0.801723	7.869252e-49	3.785115e-46
MsG0180005557.01	MsG0680031115.01	0.821257	4.446134e-53	3.598784e-50
MsG0180005557.01	MsG0680032245.01	0.810424	1.160770e-50	6.994063e-48
MsG0180005557.01	MsG0680032256.01	0.824207	9.144218e-54	8.046415e-51
MsG0180005557.01	MsG0680032413.01	0.856715	2.667434e-62	6.424055e-59
MsG0180005557.01	MsG0680032515.01	0.811011	8.665333e-51	5.304417e-48
MsG0180005557.01	MsG0680032769.01	0.833593	4.878340e-56	5.644074e-53
MsG0180005557.01	MsG0680034724.01	0.832415	9.577064e-56	1.069748e-52
MsG0180005557.01	MsG0680034729.01	0.812432	4.251868e-51	2.703159e-48
MsG0180005557.01	MsG0680035308.01	0.816058	6.726609e-52	4.717205e-49
MsG0180005557.01	MsG0680035612.01	0.838674	2.498616e-57	3.370868e-54
MsG0180005557.01	MsG0680035664.01	0.858908	5.963769e-63	1.546576e-59
MsG0180005557.01	MsG0680035665.01	0.862093	6.470097e-64	1.871811e-60
MsG0180005557.01	MsG0780037944.01	0.814214	1.726654e-51	1.151522e-48
MsG0180005557.01	MsG0780037946.01	0.818816	1.609132e-52	1.217077e-49
MsG0180005557.01	MsG0780038416.01	0.837628	4.647099e-57	6.072281e-54
MsG0180005557.01	MsG0780038417.01	0.826822	2.196410e-54	2.082599e-51
MsG0180005557.01	MsG0780038653.01	0.811005	8.689697e-51	5.318565e-48
MsG0180005557.01	MsG0780038986.01	0.806841	6.762799e-50	3.708867e-47
MsG0180005557.01	MsG0780039805.01	0.819788	9.663683e-53	7.509685e-50
MsG0180005557.01	MsG0780039986.01	0.811841	5.721051e-51	3.579286e-48
MsG0180005557.01	MsG0780040155.01	0.804613	1.985590e-49	1.028410e-46
MsG0180005557.01	MsG0780040319.01	0.800179	1.627229e-48	7.527000e-46
MsG0180005557.01	MsG0780040343.01	0.805334	1.403819e-49	7.406977e-47
MsG0180005557.01	MsG0780040350.01	0.814417	1.557225e-51	1.044340e-48
MsG0180005557.01	MsG0780040670.01	-0.820064	8.355977e-53	6.543961e-50
MsG0180005557.01	MsG0780041004.01	0.813810	2.120028e-51	1.398549e-48
MsG0180005557.01	MsG0780041442.01	0.824424	8.134835e-54	7.202092e-51
MsG0180005557.01	MsG0780041778.01	0.812736	3.647990e-51	2.337802e-48
MsG0180005557.01	MsG0880041850.01	0.800362	1.493503e-48	6.940960e-46
MsG0180005557.01	MsG0880042256.01	0.810363	1.196156e-50	7.196023e-48
MsG0180005557.01	MsG0880042343.01	0.823319	1.476760e-53	1.267212e-50
MsG0180005557.01	MsG0880043009.01	0.820254	7.557801e-53	5.949791e-50
MsG0180005557.01	MsG0880043621.01	0.803585	3.250325e-49	1.639624e-46
MsG0180005557.01	MsG0880043870.01	0.862496	4.863959e-64	1.427435e-60
MsG0180005557.01	MsG0880044105.01	0.874340	7.293550e-68	3.270877e-64
MsG0180005557.01	MsG0880044314.01	0.824160	9.383634e-54	8.246168e-51
MsG0180005557.01	MsG0880044317.01	0.836175	1.091461e-56	1.364843e-53
MsG0180005557.01	MsG0880044318.01	0.805207	1.492166e-49	7.847641e-47
MsG0180005557.01	MsG0880044411.01	0.825386	4.823462e-54	4.389786e-51
MsG0180005557.01	MsG0880044436.01	0.801492	8.778888e-49	4.198213e-46
MsG0180005557.01	MsG0880044558.01	0.805240	1.468746e-49	7.730865e-47
MsG0180005557.01	MsG0880044834.01	0.806088	9.746124e-50	5.242987e-47
MsG0180005557.01	MsG0880044884.01	0.868775	5.082591e-66	1.861283e-62
MsG0180005557.01	MsG0880045412.01	0.800844	1.190902e-48	5.602740e-46
MsG0180005557.01	MsG0880046048.01	0.804719	1.887779e-49	9.804614e-47
MsG0180005557.01	MsG0880046324.01	0.891731	3.038040e-74	2.695813e-70
MsG0180005557.01	MsG0880046408.01	0.822447	2.357283e-53	1.973628e-50
MsG0180005557.01	MsG0880046735.01	0.802674	5.014858e-49	2.471302e-46
MsG0180005557.01	MsG0880046764.01	0.808040	3.764481e-50	2.129982e-47
MsG0180005557.01	MsG0880046969.01	0.806307	8.766834e-50	4.742502e-47
MsG0180005557.01	MsG0880047111.01	0.800856	1.184204e-48	5.573004e-46
MsG0180005557.01	MsG0880047156.01	0.841373	4.942088e-58	7.247156e-55
MsG0180005557.01	MsG0880047157.01	0.827507	1.505616e-54	1.456456e-51
MsG0180005557.01	MsG0880047207.01	0.810222	1.282969e-50	7.689366e-48
MsG0180005557.01	MsG0880047435.01	0.820558	6.438002e-53	5.110961e-50
MsG0180005557.01	MsG0880047451.01	0.862518	4.789344e-64	1.406576e-60
MsG0180005557.01	MsG0880047614.01	0.807789	4.257255e-50	2.393001e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005557.01.T01	MTR_1g102780	76.963	191	9	1	1	156	10	200	2.25e-96	276
MsG0180005557.01.T01	MTR_1g102570	77.249	189	10	1	1	156	10	198	7.51e-96	275
MsG0180005557.01.T01	MTR_1g102800	77.005	187	10	1	3	156	11	197	1.30e-95	275
MsG0180005557.01.T01	MTR_1g102810	73.964	169	23	1	9	156	14	182	1.80e-85	248
MsG0180005557.01.T01	MTR_1g102830	75.301	166	20	1	12	156	21	186	7.40e-85	247

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005557.01.T01	AT3G22840	55.828	163	39	1	27	156	33	195	3.87e-58	180
MsG0180005557.01.T01	AT4G14690	69.912	113	34	0	44	156	81	193	2.71e-55	172

Find 38 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGGTGGAATTCCATGGTTCT+TGG	0.288858	1:+93256141	MsG0180005557.01.T01:CDS
GGTGGAATTCCATGGTTCTT+GGG	0.299911	1:+93256142	MsG0180005557.01.T01:CDS
GGTTTGTTTGATCAAATATC+TGG	0.314167	1:+93256118	MsG0180005557.01.T01:CDS
TATGTTGGGTTTGATTGCTT+TGG	0.357312	1:+93256282	MsG0180005557.01.T01:CDS
AATGGAAGGTTGGCTATGAT+TGG	0.393839	1:+93256055	MsG0180005557.01.T01:CDS
CCATTGATTCTCTCAGGTGC+TGG	0.411549	1:-93256037	None:intergenic
AACCTTCCATTGATTCTCTC+AGG	0.436061	1:-93256043	None:intergenic
GAATGGAAGAATAGCTATGT+TGG	0.436992	1:+93256267	MsG0180005557.01.T01:CDS
AGCTCTAGCTCTGCTAGAAA+TGG	0.453464	1:-93255510	None:intergenic
GTGGAATTCCATGGTTCTTG+GGG	0.468120	1:+93256143	MsG0180005557.01.T01:CDS
TGTCCTCAGATGCTGAGTTA+TGG	0.479277	1:+93256245	MsG0180005557.01.T01:CDS
CGGATTTGATGGCATTTGGT+GGG	0.485102	1:+93256014	MsG0180005557.01.T01:CDS
TCGGATTTGATGGCATTTGG+TGG	0.487791	1:+93256013	MsG0180005557.01.T01:CDS
AATGGAAGAATAGCTATGTT+GGG	0.501527	1:+93256268	MsG0180005557.01.T01:CDS
CCAGCACCTGAGAGAATCAA+TGG	0.516939	1:+93256037	MsG0180005557.01.T01:CDS
AACCTTATTTACCTCAGCCA+TGG	0.525798	1:-93255603	None:intergenic
CTAGCATTCCTTCTGAAGCT+AGG	0.532093	1:-93255566	None:intergenic
AGTGTGGAGTCTAAGTCAAA+AGG	0.538159	1:+93256217	MsG0180005557.01.T01:CDS
CATTAAGGAATTATTATCAA+CGG	0.541603	1:+93255442	MsG0180005557.01.T01:five_prime_UTR
GGTGGAGATAGCAAATGGAC+AGG	0.547189	1:+93256096	MsG0180005557.01.T01:CDS
TTTGATCAAATATCTGGTGG+TGG	0.559193	1:+93256124	MsG0180005557.01.T01:CDS
TGAGAGAATCAATGGAAGGT+TGG	0.560259	1:+93256045	MsG0180005557.01.T01:CDS
TAGACTCCACACTAACCCCT+TGG	0.562091	1:-93256207	None:intergenic
TCAGATGCTGAGTTATGGAA+TGG	0.568279	1:+93256250	MsG0180005557.01.T01:CDS
TTGTTTGATCAAATATCTGG+TGG	0.570609	1:+93256121	MsG0180005557.01.T01:CDS
AAGCTAGGCATGTACACAGA+AGG	0.579754	1:-93255551	None:intergenic
ATTCCATAACTCAGCATCTG+AGG	0.591329	1:-93256248	None:intergenic
CTATAGAACTCTATTACACA+AGG	0.596246	1:-93256333	None:intergenic
TAGCTTGAAAGTTCGATCCA+TGG	0.604578	1:+93255586	MsG0180005557.01.T01:CDS
ATAGAATAGTATATATTCTG+AGG	0.604681	1:-93255394	None:intergenic
AGCTAGGCATGTACACAGAA+GGG	0.614155	1:-93255550	None:intergenic
GAAAGTTCGATCCATGGCTG+AGG	0.622095	1:+93255592	MsG0180005557.01.T01:CDS
TGTACATGCCTAGCTTCAGA+AGG	0.622254	1:+93255558	MsG0180005557.01.T01:CDS
TATCTGGTGGTGGAATTCCA+TGG	0.624337	1:+93256134	MsG0180005557.01.T01:CDS
ACACTTGTCCCCAAGAACCA+TGG	0.633615	1:-93256151	None:intergenic
GTGGAGATAGCAAATGGACA+GGG	0.645295	1:+93256097	MsG0180005557.01.T01:CDS
CACCTGAGAGAATCAATGGA+AGG	0.646646	1:+93256041	MsG0180005557.01.T01:CDS
TATAGAACTCTATTACACAA+GGG	0.699610	1:-93256332	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAATATGTTTATGATTTAT+TGG	+	Chr1:93255716-93255735	MsG0180005557.01.T01:intron	10.0%
!!	TAATAATAATGATGAAAATA+TGG	+	Chr1:93255972-93255991	MsG0180005557.01.T01:intron	10.0%
!!	TATAAGATAAACAAAATTTA+AGG	+	Chr1:93255626-93255645	MsG0180005557.01.T01:intron	10.0%
!!	AAATCATAAACATATTATGT+TGG	-	Chr1:93255714-93255733	None:intergenic	15.0%
!!	ATAGAATAGTATATATTCTG+AGG	-	Chr1:93255397-93255416	None:intergenic	20.0%
!!	CATTAAGGAATTATTATCAA+CGG	+	Chr1:93255442-93255461	MsG0180005557.01.T01:five_prime_UTR	20.0%
!!!	AGTTTTGCATTTTAACATTA+AGG	+	Chr1:93255427-93255446	MsG0180005557.01.T01:intron	20.0%
!!!	ATTTTGCTAAGTACTAAATT+AGG	+	Chr1:93255922-93255941	MsG0180005557.01.T01:intron	20.0%
	AATGGAAGAATAGCTATGTT+GGG	+	Chr1:93256268-93256287	MsG0180005557.01.T01:CDS	30.0%
	TTGTTTGATCAAATATCTGG+TGG	+	Chr1:93256121-93256140	MsG0180005557.01.T01:CDS	30.0%
	TTTGTTACTATGCATACCTT+TGG	-	Chr1:93255860-93255879	None:intergenic	30.0%
!	ACTGCAATTTTTCTTTGGTT+TGG	-	Chr1:93255356-93255375	None:intergenic	30.0%
!	GCTTTTACAGAGTATGTTAA+GGG	+	Chr1:93256304-93256323	MsG0180005557.01.T01:CDS	30.0%
!	GGTTTGTTTGATCAAATATC+TGG	+	Chr1:93256118-93256137	MsG0180005557.01.T01:CDS	30.0%
!!	CTTTGATTCCATTTTTCCAA+GGG	+	Chr1:93256191-93256210	MsG0180005557.01.T01:CDS	30.0%
!!	TATTATGTTGGTGATGATGA+AGG	-	Chr1:93255702-93255721	None:intergenic	30.0%
!!	TCTTTGATTCCATTTTTCCA+AGG	+	Chr1:93256190-93256209	MsG0180005557.01.T01:CDS	30.0%
!!	TTTGATTCCATTTTTCCAAG+GGG	+	Chr1:93256192-93256211	MsG0180005557.01.T01:CDS	30.0%
!!!	CAATTTTTCTTTGGTTTGGA+AGG	-	Chr1:93255352-93255371	None:intergenic	30.0%
	GAATGGAAGAATAGCTATGT+TGG	+	Chr1:93256267-93256286	MsG0180005557.01.T01:CDS	35.0%
	GATTGCGTGTTACATTATTC+AGG	+	Chr1:93255740-93255759	MsG0180005557.01.T01:intron	35.0%
	TATGTTGGGTTTGATTGCTT+TGG	+	Chr1:93256282-93256301	MsG0180005557.01.T01:CDS	35.0%
	TTTGATCAAATATCTGGTGG+TGG	+	Chr1:93256124-93256143	MsG0180005557.01.T01:CDS	35.0%
!	CACAAAGTTTTCGGATTTGA+TGG	+	Chr1:93256003-93256022	MsG0180005557.01.T01:CDS	35.0%
!	GGCTTTTACAGAGTATGTTA+AGG	+	Chr1:93256303-93256322	MsG0180005557.01.T01:CDS	35.0%
!	GTCTCACTGCAATTTTTCTT+TGG	-	Chr1:93255361-93255380	None:intergenic	35.0%
!!	GTGATGATGATGGTGTAATT+GGG	-	Chr1:93255802-93255821	None:intergenic	35.0%
!!	TTTTCGGATTTGATGGCATT+TGG	+	Chr1:93256010-93256029	MsG0180005557.01.T01:CDS	35.0%
!!!	ATTGGTTTTGTAGCTGCTAT+GGG	+	Chr1:93256073-93256092	MsG0180005557.01.T01:CDS	35.0%
	ACACTAACCCCTTGGAAAAA+TGG	-	Chr1:93256202-93256221	None:intergenic	40.0%
	AGCAGAAAGAGCAACCAAAA+AGG	+	Chr1:93255766-93255785	MsG0180005557.01.T01:intron	40.0%
	AGTGTGGAGTCTAAGTCAAA+AGG	+	Chr1:93256217-93256236	MsG0180005557.01.T01:CDS	40.0%
	ATCCATGGCTGAGGTAAATA+AGG	+	Chr1:93255601-93255620	MsG0180005557.01.T01:intron	40.0%
	ATTCCATAACTCAGCATCTG+AGG	-	Chr1:93256251-93256270	None:intergenic	40.0%
	TAGCTTGAAAGTTCGATCCA+TGG	+	Chr1:93255586-93255605	MsG0180005557.01.T01:CDS	40.0%
	TCAGATGCTGAGTTATGGAA+TGG	+	Chr1:93256250-93256269	MsG0180005557.01.T01:CDS	40.0%
	TGAGAGAATCAATGGAAGGT+TGG	+	Chr1:93256045-93256064	MsG0180005557.01.T01:CDS	40.0%
!	AACCTTATTTACCTCAGCCA+TGG	-	Chr1:93255606-93255625	None:intergenic	40.0%
!	AATGGAAGGTTGGCTATGAT+TGG	+	Chr1:93256055-93256074	MsG0180005557.01.T01:CDS	40.0%
!	GCAGATGAGCACAAAGTTTT+CGG	+	Chr1:93255994-93256013	MsG0180005557.01.T01:intron	40.0%
!	GGTGATGATGAAGGTGTAAT+CGG	-	Chr1:93255693-93255712	None:intergenic	40.0%
!!	AACCTTCCATTGATTCTCTC+AGG	-	Chr1:93256046-93256065	None:intergenic	40.0%
!!	ATTTTTCCAAGGGGTTAGTG+TGG	+	Chr1:93256201-93256220	MsG0180005557.01.T01:CDS	40.0%
!!	GGTGATGATGATGGTGTAAT+TGG	-	Chr1:93255803-93255822	None:intergenic	40.0%
!!!	GATTGGTTTTGTAGCTGCTA+TGG	+	Chr1:93256072-93256091	MsG0180005557.01.T01:CDS	40.0%
!!!	TTGGTTTTGTAGCTGCTATG+GGG	+	Chr1:93256074-93256093	MsG0180005557.01.T01:CDS	40.0%
	AAGCTAGGCATGTACACAGA+AGG	-	Chr1:93255554-93255573	None:intergenic	45.0%
	AGCTAGGCATGTACACAGAA+GGG	-	Chr1:93255553-93255572	None:intergenic	45.0%
	CACCTGAGAGAATCAATGGA+AGG	+	Chr1:93256041-93256060	MsG0180005557.01.T01:CDS	45.0%
	GATGGTGTAATTGGGTCTAC+AGG	-	Chr1:93255794-93255813	None:intergenic	45.0%
	GGTGGAATTCCATGGTTCTT+GGG	+	Chr1:93256142-93256161	MsG0180005557.01.T01:CDS	45.0%
	GTGGAATTCCATGGTTCTTG+GGG	+	Chr1:93256143-93256162	MsG0180005557.01.T01:CDS	45.0%
	GTGGAGATAGCAAATGGACA+GGG	+	Chr1:93256097-93256116	MsG0180005557.01.T01:CDS	45.0%
	TGGTGGAATTCCATGGTTCT+TGG	+	Chr1:93256141-93256160	MsG0180005557.01.T01:CDS	45.0%
	TGTACATGCCTAGCTTCAGA+AGG	+	Chr1:93255558-93255577	MsG0180005557.01.T01:CDS	45.0%
	TGTCCTCAGATGCTGAGTTA+TGG	+	Chr1:93256245-93256264	MsG0180005557.01.T01:CDS	45.0%
!	CGGATTTGATGGCATTTGGT+GGG	+	Chr1:93256014-93256033	MsG0180005557.01.T01:CDS	45.0%
!	TCGGATTTGATGGCATTTGG+TGG	+	Chr1:93256013-93256032	MsG0180005557.01.T01:CDS	45.0%
!!	AGCTCTAGCTCTGCTAGAAA+TGG	-	Chr1:93255513-93255532	None:intergenic	45.0%
!!	CTAGCATTCCTTCTGAAGCT+AGG	-	Chr1:93255569-93255588	None:intergenic	45.0%
!!	GTTGGTGTTGGTGATGATGA+TGG	-	Chr1:93255812-93255831	None:intergenic	45.0%
!!	TATCTGGTGGTGGAATTCCA+TGG	+	Chr1:93256134-93256153	MsG0180005557.01.T01:CDS	45.0%
!!!	TGGTTTTGTAGCTGCTATGG+GGG	+	Chr1:93256075-93256094	MsG0180005557.01.T01:CDS	45.0%
!!	CATTATTATTATTTATTAAA+AGG	-	Chr1:93255963-93255982	None:intergenic	5.0%
	ACACTTGTCCCCAAGAACCA+TGG	-	Chr1:93256154-93256173	None:intergenic	50.0%
	ATGGGGGTGGAGATAGCAAA+TGG	+	Chr1:93256091-93256110	MsG0180005557.01.T01:CDS	50.0%
	CCAGCACCTGAGAGAATCAA+TGG	+	Chr1:93256037-93256056	MsG0180005557.01.T01:CDS	50.0%
	GAAAGTTCGATCCATGGCTG+AGG	+	Chr1:93255592-93255611	MsG0180005557.01.T01:CDS	50.0%
	GGTGGAGATAGCAAATGGAC+AGG	+	Chr1:93256096-93256115	MsG0180005557.01.T01:CDS	50.0%
	TACCTTTGGTGAGCGAGTGT+AGG	-	Chr1:93255846-93255865	None:intergenic	50.0%
	TAGACTCCACACTAACCCCT+TGG	-	Chr1:93256210-93256229	None:intergenic	50.0%
!	TGGGTCTACAGGCACCTTTT+TGG	-	Chr1:93255783-93255802	None:intergenic	50.0%
!	TTTTGTAGCTGCTATGGGGG+TGG	+	Chr1:93256078-93256097	MsG0180005557.01.T01:CDS	50.0%
!!	CCATTGATTCTCTCAGGTGC+TGG	-	Chr1:93256040-93256059	None:intergenic	50.0%
	AGCCTACACTCGCTCACCAA+AGG	+	Chr1:93255841-93255860	MsG0180005557.01.T01:intron	55.0%
	TTTGGTGAGCGAGTGTAGGC+TGG	-	Chr1:93255842-93255861	None:intergenic	55.0%
!	GTGTAGGCTGGTTGTGGTGT+TGG	-	Chr1:93255830-93255849	None:intergenic	55.0%
!!	GCTGGTTGTGGTGTTGGTGT+TGG	-	Chr1:93255824-93255843	None:intergenic	55.0%
!	GAGCGAGTGTAGGCTGGTTG+TGG	-	Chr1:93255836-93255855	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	93255344	93256342	93255344	ID=MsG0180005557.01;Name=MsG0180005557.01
Chr1	mRNA	93255344	93256342	93255344	ID=MsG0180005557.01.T01;Parent=MsG0180005557.01;Name=MsG0180005557.01.T01;_AED=0.50;_eAED=0.50;_QI=122\|0.5\|0.66\|1\|0\|0.33\|3\|0\|156
Chr1	exon	93255344	93255406	93255344	ID=MsG0180005557.01.T01:exon:16068;Parent=MsG0180005557.01.T01
Chr1	exon	93255429	93255613	93255429	ID=MsG0180005557.01.T01:exon:16069;Parent=MsG0180005557.01.T01
Chr1	exon	93255998	93256342	93255998	ID=MsG0180005557.01.T01:exon:16070;Parent=MsG0180005557.01.T01
Chr1	five_prime_UTR	93255344	93255406	93255344	ID=MsG0180005557.01.T01:five_prime_utr;Parent=MsG0180005557.01.T01
Chr1	five_prime_UTR	93255429	93255487	93255429	ID=MsG0180005557.01.T01:five_prime_utr;Parent=MsG0180005557.01.T01
Chr1	CDS	93255488	93255613	93255488	ID=MsG0180005557.01.T01:cds;Parent=MsG0180005557.01.T01
Chr1	CDS	93255998	93256342	93255998	ID=MsG0180005557.01.T01:cds;Parent=MsG0180005557.01.T01

Gene Sequence

>MsG0180005557.01.T01

ATGTCGTCAAGCTCATTGAGCACCATTTCTAGCAGAGCTAGAGCTAGCAAATTCAGCAACATCCCTTCTGTGTACATGCCTAGCTTCAGAAGGAATGCTAGCTTGAAAGTTCGATCCATGGCTGAGATGAGCACAAAGTTTTCGGATTTGATGGCATTTGGTGGGCCAGCACCTGAGAGAATCAATGGAAGGTTGGCTATGATTGGTTTTGTAGCTGCTATGGGGGTGGAGATAGCAAATGGACAGGGTTTGTTTGATCAAATATCTGGTGGTGGAATTCCATGGTTCTTGGGGACAAGTGTGTTGTTGACACTTGCTTCTTTGATTCCATTTTTCCAAGGGGTTAGTGTGGAGTCTAAGTCAAAAGGTGTCATGTCCTCAGATGCTGAGTTATGGAATGGAAGAATAGCTATGTTGGGTTTGATTGCTTTGGCTTTTACAGAGTATGTTAAGGGTACTTCCCTTGTGTAA

Protein sequence

>MsG0180005557.01.T01

MSSSSLSTISSRARASKFSNIPSVYMPSFRRNASLKVRSMAEMSTKFSDLMAFGGPAPERINGRLAMIGFVAAMGVEIANGQGLFDQISGGGIPWFLGTSVLLTLASLIPFFQGVSVESKSKGVMSSDAELWNGRIAMLGLIALAFTEYVKGTSLV*