Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016301.01.T01 | XP_013461269.1 | 96.226 | 106 | 4 | 0 | 1 | 106 | 94 | 199 | 1.34E-68 | 214 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016301.01.T01 | A0A072UZT9 | 96.226 | 106 | 4 | 0 | 1 | 106 | 94 | 199 | 6.42e-69 | 214 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048134.01 | MsG0380016301.01 | 0.802517 | 5.402993e-49 | 2.651924e-46 |
| MsG0180003405.01 | MsG0380016301.01 | -0.809599 | 1.747166e-50 | 1.030276e-47 |
| MsG0180004121.01 | MsG0380016301.01 | 0.824676 | 7.096357e-54 | 6.329680e-51 |
| MsG0180004232.01 | MsG0380016301.01 | 0.820461 | 6.775572e-53 | 5.364779e-50 |
| MsG0180004359.01 | MsG0380016301.01 | 0.806715 | 7.189628e-50 | 3.930093e-47 |
| MsG0380011968.01 | MsG0380016301.01 | 0.826499 | 2.623552e-54 | 2.464598e-51 |
| MsG0380014344.01 | MsG0380016301.01 | 0.810338 | 1.211418e-50 | 7.282680e-48 |
| MsG0380014639.01 | MsG0380016301.01 | -0.801205 | 1.005045e-48 | 4.771534e-46 |
| MsG0380015660.01 | MsG0380016301.01 | 0.806310 | 8.751311e-50 | 4.734522e-47 |
| MsG0380016299.01 | MsG0380016301.01 | 0.873677 | 1.221439e-67 | 5.346243e-64 |
| MsG0380016300.01 | MsG0380016301.01 | 0.865682 | 4.947925e-65 | 1.624052e-61 |
| MsG0380016301.01 | MsG0480019146.01 | 0.827064 | 1.922812e-54 | 1.836106e-51 |
| MsG0380016301.01 | MsG0480019842.01 | 0.806203 | 9.221077e-50 | 4.975251e-47 |
| MsG0380016301.01 | MsG0480020904.01 | 0.825963 | 3.519113e-54 | 3.256064e-51 |
| MsG0380016301.01 | MsG0480021129.01 | 0.805512 | 1.288036e-49 | 6.827134e-47 |
| MsG0380016301.01 | MsG0480021360.01 | 0.848272 | 6.811543e-60 | 1.243348e-56 |
| MsG0380016301.01 | MsG0480021361.01 | 0.838415 | 2.914817e-57 | 3.901424e-54 |
| MsG0380016301.01 | MsG0480022055.01 | 0.807273 | 5.479699e-50 | 3.039070e-47 |
| MsG0380016301.01 | MsG0480023432.01 | 0.803965 | 2.709734e-49 | 1.380308e-46 |
| MsG0380016301.01 | MsG0480023987.01 | 0.807991 | 3.857452e-50 | 2.179758e-47 |
| MsG0380016301.01 | MsG0580024087.01 | 0.813447 | 2.548491e-51 | 1.664741e-48 |
| MsG0380016301.01 | MsG0580025507.01 | 0.803174 | 3.954078e-49 | 1.973603e-46 |
| MsG0380016301.01 | MsG0580025590.01 | 0.807050 | 6.108245e-50 | 3.367987e-47 |
| MsG0380016301.01 | MsG0680031474.01 | 0.802523 | 5.388841e-49 | 2.645307e-46 |
| MsG0380016301.01 | MsG0680031684.01 | 0.808194 | 3.490656e-50 | 1.983195e-47 |
| MsG0380016301.01 | MsG0680034418.01 | 0.807904 | 4.025325e-50 | 2.269234e-47 |
| MsG0380016301.01 | MsG0680034728.01 | 0.825298 | 5.058607e-54 | 4.592594e-51 |
| MsG0380016301.01 | MsG0680035584.01 | -0.802841 | 4.631870e-49 | 2.292480e-46 |
| MsG0380016301.01 | MsG0780036199.01 | 0.801476 | 8.845509e-49 | 4.228362e-46 |
| MsG0380016301.01 | MsG0780036839.01 | 0.839606 | 1.433309e-57 | 1.989503e-54 |
| MsG0380016301.01 | MsG0780038519.01 | 0.824899 | 6.285430e-54 | 5.642245e-51 |
| MsG0380016301.01 | MsG0780039237.01 | 0.803805 | 2.925215e-49 | 1.483911e-46 |
| MsG0380016301.01 | MsG0780039855.01 | 0.807547 | 4.793637e-50 | 2.677478e-47 |
| MsG0380016301.01 | MsG0780039883.01 | 0.805466 | 1.317080e-49 | 6.973147e-47 |
| MsG0380016301.01 | MsG0780039979.01 | 0.822721 | 2.035856e-53 | 1.717836e-50 |
| MsG0380016301.01 | MsG0780040497.01 | 0.808030 | 3.783442e-50 | 2.140138e-47 |
| MsG0380016301.01 | MsG0780040498.01 | 0.827471 | 1.536056e-54 | 1.484408e-51 |
| MsG0380016301.01 | MsG0780040598.01 | 0.803421 | 3.515170e-49 | 1.765682e-46 |
| MsG0380016301.01 | MsG0780040784.01 | 0.808058 | 3.732196e-50 | 2.112678e-47 |
| MsG0380016301.01 | MsG0880043212.01 | 0.825148 | 5.490295e-54 | 4.963534e-51 |
| MsG0380016301.01 | MsG0880043217.01 | 0.812889 | 3.377843e-51 | 2.173364e-48 |
| MsG0380016301.01 | MsG0880043906.01 | 0.803957 | 2.720619e-49 | 1.385573e-46 |
| MsG0380016301.01 | MsG0880045854.01 | 0.808443 | 3.089233e-50 | 1.766680e-47 |
| MsG0380016301.01 | MsG0880046271.01 | 0.824558 | 7.565142e-54 | 6.724351e-51 |
| MsG0380016301.01 | MsG0880046932.01 | 0.811271 | 7.610253e-51 | 4.689982e-48 |
| MsG0380016301.01 | MsG0880047383.01 | 0.819908 | 9.069274e-53 | 7.071589e-50 |
| MsG0280006546.01 | MsG0380016301.01 | 0.801294 | 9.635482e-49 | 4.584990e-46 |
| MsG0280007937.01 | MsG0380016301.01 | 0.800927 | 1.145689e-48 | 5.401367e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016301.01.T01 | MTR_3g087278 | 96.226 | 106 | 4 | 0 | 1 | 106 | 94 | 199 | 1.63e-72 | 214 |
| MsG0380016301.01.T01 | MTR_3g087270 | 92.453 | 106 | 8 | 0 | 1 | 106 | 94 | 199 | 2.25e-70 | 209 |
| MsG0380016301.01.T01 | MTR_3g087280 | 85.849 | 106 | 15 | 0 | 1 | 106 | 94 | 199 | 3.29e-66 | 198 |
| MsG0380016301.01.T01 | MTR_3g087325 | 85.849 | 106 | 15 | 0 | 1 | 106 | 94 | 199 | 2.17e-64 | 193 |
| MsG0380016301.01.T01 | MTR_3g087330 | 83.962 | 106 | 17 | 0 | 1 | 106 | 94 | 199 | 1.85e-62 | 189 |
| MsG0380016301.01.T01 | MTR_3g087275 | 83.962 | 106 | 16 | 1 | 1 | 106 | 94 | 198 | 1.35e-60 | 184 |
| MsG0380016301.01.T01 | MTR_3g087300 | 72.115 | 104 | 11 | 1 | 1 | 104 | 68 | 153 | 2.20e-49 | 155 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAAGAGAAGGTTGAATAAT+TGG | 0.248389 | 3:-81679443 | MsG0380016301.01.T01:CDS |
| TTAATTTCCACTTCCACTTC+TGG | 0.251842 | 3:+81679504 | None:intergenic |
| TGCGCTTGGTGCAGCTTTAG+AGG | 0.317354 | 3:-81679352 | MsG0380016301.01.T01:CDS |
| AAAATGGTTGAAAATGGATT+TGG | 0.402081 | 3:-81679565 | MsG0380016301.01.T01:CDS |
| CGCAAATAAATCACAATCTT+CGG | 0.408596 | 3:+81679371 | None:intergenic |
| TCCACTTCCACTTCTGGTGT+TGG | 0.429577 | 3:+81679510 | None:intergenic |
| AGTGACTTCGGTTGCTGAGA+TGG | 0.471131 | 3:-81679475 | MsG0380016301.01.T01:CDS |
| GATTCAAGAATCAGTTTCCA+TGG | 0.479905 | 3:+81679606 | None:intergenic |
| TTGGCAAAGGTGAGATTCCA+TGG | 0.486019 | 3:-81679623 | MsG0380016301.01.T01:CDS |
| GATTGTGATTTATTTGCGCT+TGG | 0.539399 | 3:-81679366 | MsG0380016301.01.T01:CDS |
| GCCAACACCAGAAGTGGAAG+TGG | 0.540390 | 3:-81679511 | MsG0380016301.01.T01:CDS |
| TGGAAAAGAAGAATAAGAGA+AGG | 0.547734 | 3:-81679455 | MsG0380016301.01.T01:CDS |
| AATTAAAATAAGAGTGACTT+CGG | 0.548743 | 3:-81679487 | MsG0380016301.01.T01:CDS |
| TTGAAAATGGATTTGGTGAG+TGG | 0.556088 | 3:-81679558 | MsG0380016301.01.T01:CDS |
| GATTCTTGAATCACAAAACA+TGG | 0.569666 | 3:-81679595 | MsG0380016301.01.T01:CDS |
| TTGAATAATTGGAATGAGTG+TGG | 0.570637 | 3:-81679432 | MsG0380016301.01.T01:CDS |
| TATGTTGCCAACACCAGAAG+TGG | 0.592676 | 3:-81679517 | MsG0380016301.01.T01:CDS |
| GGGTCACAACTTATTGGCAA+AGG | 0.622442 | 3:-81679636 | MsG0380016301.01.T01:CDS |
| ATGGTCATGATCATAATGCT+TGG | 0.627084 | 3:-81679401 | MsG0380016301.01.T01:CDS |
| TAAGGCAAAGGAAGATGTGG+GGG | 0.660678 | 3:-81679656 | None:intergenic |
| CTAAGGCAAAGGAAGATGTG+GGG | 0.689053 | 3:-81679657 | None:intergenic |
| GAGTGGAAGTGATTGCAAAG+AGG | 0.732454 | 3:-81679541 | MsG0380016301.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTAAAATAAGAGTGACTT+CGG | - | Chr3:81679499-81679518 | MsG0380016301.01.T01:CDS | 20.0% |
| ! | AAAACATGGAGCTAAAAAAA+TGG | - | Chr3:81679405-81679424 | MsG0380016301.01.T01:CDS | 25.0% |
| ! | ATAAGAGAAGGTTGAATAAT+TGG | - | Chr3:81679543-81679562 | MsG0380016301.01.T01:CDS | 25.0% |
| ! | GCTAAAAAAATGGTTGAAAA+TGG | - | Chr3:81679415-81679434 | MsG0380016301.01.T01:CDS | 25.0% |
| !! | AAAATGGTTGAAAATGGATT+TGG | - | Chr3:81679421-81679440 | MsG0380016301.01.T01:CDS | 25.0% |
| CGCAAATAAATCACAATCTT+CGG | + | Chr3:81679618-81679637 | None:intergenic | 30.0% | |
| GATTCTTGAATCACAAAACA+TGG | - | Chr3:81679391-81679410 | MsG0380016301.01.T01:CDS | 30.0% | |
| TGGAAAAGAAGAATAAGAGA+AGG | - | Chr3:81679531-81679550 | MsG0380016301.01.T01:CDS | 30.0% | |
| TTGAATAATTGGAATGAGTG+TGG | - | Chr3:81679554-81679573 | MsG0380016301.01.T01:CDS | 30.0% | |
| AATGAGTGTGGATGCAAAAA+TGG | - | Chr3:81679566-81679585 | MsG0380016301.01.T01:CDS | 35.0% | |
| ATGGTCATGATCATAATGCT+TGG | - | Chr3:81679585-81679604 | MsG0380016301.01.T01:CDS | 35.0% | |
| GATTCAAGAATCAGTTTCCA+TGG | + | Chr3:81679383-81679402 | None:intergenic | 35.0% | |
| GATTGTGATTTATTTGCGCT+TGG | - | Chr3:81679620-81679639 | MsG0380016301.01.T01:CDS | 35.0% | |
| TTAATTTCCACTTCCACTTC+TGG | + | Chr3:81679485-81679504 | None:intergenic | 35.0% | |
| !! | TTGAAAATGGATTTGGTGAG+TGG | - | Chr3:81679428-81679447 | MsG0380016301.01.T01:CDS | 35.0% |
| ATGTGGGGGTCACAACTTAT+TGG | - | Chr3:81679344-81679363 | MsG0380016301.01.T01:CDS | 45.0% | |
| GAGTGGAAGTGATTGCAAAG+AGG | - | Chr3:81679445-81679464 | MsG0380016301.01.T01:CDS | 45.0% | |
| GGGTCACAACTTATTGGCAA+AGG | - | Chr3:81679350-81679369 | MsG0380016301.01.T01:CDS | 45.0% | |
| TATGTTGCCAACACCAGAAG+TGG | - | Chr3:81679469-81679488 | MsG0380016301.01.T01:CDS | 45.0% | |
| TTGGCAAAGGTGAGATTCCA+TGG | - | Chr3:81679363-81679382 | MsG0380016301.01.T01:CDS | 45.0% | |
| AGTGACTTCGGTTGCTGAGA+TGG | - | Chr3:81679511-81679530 | MsG0380016301.01.T01:CDS | 50.0% | |
| TCCACTTCCACTTCTGGTGT+TGG | + | Chr3:81679479-81679498 | None:intergenic | 50.0% | |
| GCCAACACCAGAAGTGGAAG+TGG | - | Chr3:81679475-81679494 | MsG0380016301.01.T01:CDS | 55.0% | |
| !! | TGCGCTTGGTGCAGCTTTAG+AGG | - | Chr3:81679634-81679653 | MsG0380016301.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 81679344 | 81679664 | 81679344 | ID=MsG0380016301.01;Name=MsG0380016301.01 |
| Chr3 | mRNA | 81679344 | 81679664 | 81679344 | ID=MsG0380016301.01.T01;Parent=MsG0380016301.01;Name=MsG0380016301.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|106 |
| Chr3 | exon | 81679344 | 81679664 | 81679344 | ID=MsG0380016301.01.T01:exon:26308;Parent=MsG0380016301.01.T01 |
| Chr3 | CDS | 81679344 | 81679664 | 81679344 | ID=MsG0380016301.01.T01:cds;Parent=MsG0380016301.01.T01 |
| Gene Sequence |
| Protein sequence |
