Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025507.01.T01 | AES95816.1 | 96.707 | 334 | 11 | 0 | 13 | 346 | 1 | 334 | 0 | 663 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047967.01 | MsG0580025507.01 | -0.812268 | 4.618709e-51 | 2.923324e-48 |
| MsG0080048036.01 | MsG0580025507.01 | 0.812560 | 3.986422e-51 | 2.542852e-48 |
| MsG0080048059.01 | MsG0580025507.01 | -0.814204 | 1.735600e-51 | 1.157168e-48 |
| MsG0080048347.01 | MsG0580025507.01 | 0.822731 | 2.024485e-53 | 1.708771e-50 |
| MsG0080048352.01 | MsG0580025507.01 | 0.818690 | 1.718591e-52 | 1.295363e-49 |
| MsG0080048486.01 | MsG0580025507.01 | 0.832900 | 7.259439e-56 | 8.226580e-53 |
| MsG0080048487.01 | MsG0580025507.01 | 0.829484 | 5.015781e-55 | 5.140318e-52 |
| MsG0080048488.01 | MsG0580025507.01 | 0.824129 | 9.540078e-54 | 8.376145e-51 |
| MsG0080048489.01 | MsG0580025507.01 | 0.830359 | 3.069536e-55 | 3.226780e-52 |
| MsG0080048595.01 | MsG0580025507.01 | 0.804074 | 2.572424e-49 | 1.314044e-46 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025507.01.T01 | MTR_5g029840 | 96.707 | 334 | 11 | 0 | 13 | 346 | 1 | 334 | 0.0 | 663 |
| MsG0580025507.01.T01 | MTR_4g478180 | 44.110 | 365 | 155 | 9 | 12 | 346 | 1 | 346 | 4.80e-86 | 264 |
| MsG0580025507.01.T01 | MTR_3g074520 | 61.828 | 186 | 61 | 2 | 13 | 188 | 1 | 186 | 2.73e-80 | 249 |
| MsG0580025507.01.T01 | MTR_5g007370 | 43.860 | 342 | 141 | 12 | 13 | 346 | 1 | 299 | 1.77e-74 | 232 |
| MsG0580025507.01.T01 | MTR_2g089620 | 70.968 | 155 | 45 | 0 | 13 | 167 | 1 | 155 | 9.75e-74 | 231 |
| MsG0580025507.01.T01 | MTR_2g011660 | 77.692 | 130 | 28 | 1 | 13 | 141 | 1 | 130 | 1.80e-71 | 226 |
| MsG0580025507.01.T01 | MTR_4g091490 | 43.385 | 325 | 135 | 8 | 13 | 299 | 1 | 314 | 1.50e-70 | 224 |
| MsG0580025507.01.T01 | MTR_6g012690 | 71.756 | 131 | 37 | 0 | 13 | 143 | 1 | 131 | 3.23e-70 | 223 |
| MsG0580025507.01.T01 | MTR_7g011170 | 66.207 | 145 | 45 | 1 | 13 | 153 | 1 | 145 | 1.96e-69 | 219 |
| MsG0580025507.01.T01 | MTR_4g082290 | 69.925 | 133 | 40 | 0 | 13 | 145 | 1 | 133 | 1.85e-68 | 220 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025507.01.T01 | AT5G16770 | 49.288 | 351 | 135 | 11 | 13 | 346 | 1 | 325 | 4.93e-95 | 286 |
| MsG0580025507.01.T01 | AT5G16770 | 49.288 | 351 | 135 | 11 | 13 | 346 | 1 | 325 | 4.93e-95 | 286 |
| MsG0580025507.01.T01 | AT5G16770 | 49.288 | 351 | 135 | 11 | 13 | 346 | 1 | 325 | 1.45e-94 | 285 |
| MsG0580025507.01.T01 | AT5G16770 | 49.288 | 351 | 135 | 11 | 13 | 346 | 1 | 325 | 1.45e-94 | 285 |
| MsG0580025507.01.T01 | AT3G02940 | 45.402 | 348 | 138 | 8 | 13 | 346 | 1 | 310 | 6.52e-89 | 270 |
| MsG0580025507.01.T01 | AT3G02940 | 45.402 | 348 | 138 | 8 | 13 | 346 | 1 | 310 | 1.25e-88 | 269 |
| MsG0580025507.01.T01 | AT3G02940 | 45.402 | 348 | 138 | 8 | 13 | 346 | 1 | 310 | 1.25e-88 | 269 |
| MsG0580025507.01.T01 | AT4G17785 | 44.780 | 364 | 156 | 15 | 13 | 346 | 1 | 349 | 2.35e-82 | 254 |
| MsG0580025507.01.T01 | AT4G17785 | 44.505 | 364 | 157 | 15 | 13 | 346 | 1 | 349 | 8.97e-82 | 253 |
| MsG0580025507.01.T01 | AT1G34670 | 63.842 | 177 | 60 | 2 | 13 | 187 | 1 | 175 | 8.08e-79 | 246 |
Find 76 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATTGCAGCTCATCTTCC+AGG | 0.357488 | 5:+19766353 | MsG0580025507.01.T01:CDS |
| AAACATGGCCATGGAAATTG+GGG | 0.562809 | 5:+19765912 | MsG0580025507.01.T01:CDS |
| AAAGTGTTCCCCAATTTCCA+TGG | 0.538619 | 5:-19765920 | None:intergenic |
| AAATGCAATCTTTGATGAAT+GGG | 0.387848 | 5:+19767006 | MsG0580025507.01.T01:CDS |
| AAATTGAATGGATTAAAGCT+AGG | 0.500163 | 5:-19766680 | None:intergenic |
| AACCCTAGTTTATACTCTTA+TGG | 0.226899 | 5:+19766776 | MsG0580025507.01.T01:CDS |
| AAGCTGGATATTTCAAAAGG+TGG | 0.539184 | 5:+19766815 | MsG0580025507.01.T01:CDS |
| AATAATTCATTTGGTAACTT+GGG | 0.423937 | 5:+19766623 | MsG0580025507.01.T01:CDS |
| AATCAAGTGGTGGCTCCTAC+TGG | 0.398129 | 5:+19767047 | MsG0580025507.01.T01:CDS |
| AATGAGCCATTAAGTTTGAG+TGG | 0.592575 | 5:+19766734 | MsG0580025507.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACCCTAGTTTATACTCTTA+TGG | + | Chr5:19766776-19766795 | MsG0580025507.01.T01:CDS | 30.0% | |
| AATTATCCTTATAACCCCTT+AGG | + | Chr5:19766647-19766666 | MsG0580025507.01.T01:CDS | 30.0% | |
| ATGAGATTGGTGTAAAGAAA+GGG | + | Chr5:19765844-19765863 | MsG0580025507.01.T01:CDS | 30.0% | |
| ATGAGTCTTTCAAACTTACT+TGG | + | Chr5:19766488-19766507 | MsG0580025507.01.T01:CDS | 30.0% | |
| ATTATCCTTATAACCCCTTA+GGG | + | Chr5:19766648-19766667 | MsG0580025507.01.T01:CDS | 30.0% | |
| CAAATGCAATCTTTGATGAA+TGG | + | Chr5:19767005-19767024 | MsG0580025507.01.T01:CDS | 30.0% | |
| GTAAGTTTGAAAGACTCATA+AGG | - | Chr5:19766487-19766506 | None:intergenic | 30.0% | |
| TCAAAGCTGGATATTTCAAA+AGG | + | Chr5:19766812-19766831 | MsG0580025507.01.T01:CDS | 30.0% | |
| TCATCAAAGATTGCATTTGA+AGG | - | Chr5:19767004-19767023 | None:intergenic | 30.0% | |
| TGATTGAAAATTCCAGTTCT+AGG | - | Chr5:19766935-19766954 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 19765786 | 19767087 | 19765786 | ID=MsG0580025507.01;Name=MsG0580025507.01 |
| Chr5 | mRNA | 19765786 | 19767087 | 19765786 | ID=MsG0580025507.01.T01;Parent=MsG0580025507.01;Name=MsG0580025507.01.T01;_AED=0.06;_eAED=0.06;_QI=0|0|0|1|1|1|2|0|346 |
| Chr5 | exon | 19765786 | 19766084 | 19765786 | ID=MsG0580025507.01.T01:exon:8332;Parent=MsG0580025507.01.T01 |
| Chr5 | exon | 19766346 | 19767087 | 19766346 | ID=MsG0580025507.01.T01:exon:8333;Parent=MsG0580025507.01.T01 |
| Chr5 | CDS | 19765786 | 19766084 | 19765786 | ID=MsG0580025507.01.T01:cds;Parent=MsG0580025507.01.T01 |
| Chr5 | CDS | 19766346 | 19767087 | 19766346 | ID=MsG0580025507.01.T01:cds;Parent=MsG0580025507.01.T01 |
| Gene Sequence |
>MsG0580025507.01.T01 TTGTCTTGGAACATTAATTCACTGTGCAATATTATTATGGGTAGGTCACCATGTTGTGATGAGATTGGTGTAAAGAAAGGGCCATGGACACAAGAAGAAGATGAGAAGCTGATTGATTATATCAACAAACATGGCCATGGAAATTGGGGAACACTTTCAAAGCGTGCTGGTTTGAATAGGTGTGGGAAGAGTTGTAGGTTAAGGTGGACAAATTATTTGAGACCTGATATCAAGAGAGGGAAATTCACTGATGAAGAAGAAAGAGTTATCATCAACCTTCATTCAGTACTTGGAAACAAGTGGTCGAAAATTGCAGCTCATCTTCCAGGACGAACAGATAACGAGATAAAGAATTATTGGAACACAAACATAAGGAAAAAGCTTCTCAAGATGGGAATTGATCCTGAAACTCACAAGCCAAGAACTGATTACAATCACCTTATGAGTCTTTCAAACTTACTTGGCATGTCAAATATAGGCAATACTTTTAGCAACAACCCTCTTGGTTTACAACAAGATATTACCCACCTAGCCAAAATGCAATTGTTGCAAAATATGTTGCAAATTATGAATACCAATAATTCATTTGGTAACTTGGGGAATTATCCTTATAACCCCTTAGGGAATATTAACCCTAGCTTTAATCCATTCAATTTGTTTTTGAATGGAACAAACACAATTCAAACTAATGAGCCATTAAGTTTGAGTGGTGGTGAAGAATATGCTATGAACCCTAGTTTATACTCTTATGGACAAAGTGAGTCTTCAAAGCTGGATATTTCAAAAGGTGGATCAAGTTTACAAGATCTTGATTATAGCAAAATGGGAAGCATTTCATGTGAAAATCAAGAAGAAAATCTACTTCCTGCTTTGGTTGCTTCCTCTCCTAGAACTGGAATTTTCAATCAAATGGAAAATGATTGTAACAAAGCTCAAATGTCCATTGATCACTCACCTTCAAATGCAATCTTTGATGAATGGGAGAAGTTCCTTGATGATGAATCAAGTGGTGGCTCCTACTGGAAAGAGCTTTTAGAGTAA |
| Protein sequence |
>MsG0580025507.01.T01 LSWNINSLCNIIMGRSPCCDEIGVKKGPWTQEEDEKLIDYINKHGHGNWGTLSKRAGLNRCGKSCRLRWTNYLRPDIKRGKFTDEEERVIINLHSVLGNKWSKIAAHLPGRTDNEIKNYWNTNIRKKLLKMGIDPETHKPRTDYNHLMSLSNLLGMSNIGNTFSNNPLGLQQDITHLAKMQLLQNMLQIMNTNNSFGNLGNYPYNPLGNINPSFNPFNLFLNGTNTIQTNEPLSLSGGEEYAMNPSLYSYGQSESSKLDISKGGSSLQDLDYSKMGSISCENQEENLLPALVASSPRTGIFNQMENDCNKAQMSIDHSPSNAIFDEWEKFLDDESSGGSYWKELLE* |
