AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380014530.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014530.01.T01 MTR_3g058890 88.000 150 17 1 1 149 72 221 1.54e-63 194
MsG0380014530.01.T01 MTR_5g095540 36.683 199 69 7 1 149 75 266 1.12e-13 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 21 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGAAGTAGCATATTGGTTT+TGG 0.246095 3:+56134816 MsG0380014530.01.T01:CDS
GAAGAAGAAAGTGGGCTATT+TGG 0.290215 3:+56134856 MsG0380014530.01.T01:CDS
ATATTCTCTTCATTATTATT+AGG 0.299233 3:-56134724 None:intergenic
TTCATTATTATTAGGCTCAT+TGG 0.340053 3:-56134716 None:intergenic
GAATTACAAGATCTTTATCT+TGG 0.366363 3:+56134635 MsG0380014530.01.T01:CDS
CATTGGTTCTTTAACATAGT+TGG 0.371362 3:-56134680 None:intergenic
AGGAAAAGGAAGTAGCATAT+TGG 0.398055 3:+56134810 MsG0380014530.01.T01:CDS
AAAAGATGAAGAAAGATTTG+AGG 0.399049 3:+56135007 MsG0380014530.01.T01:CDS
CAAGGAGACCATTGCATATG+AGG 0.401362 3:-56134603 None:intergenic
GGAAGATTCATCAACGCTAC+TGG 0.445007 3:-56134659 None:intergenic
GAAAGTGTTGAAGAAGGAAA+AGG 0.464464 3:+56134796 MsG0380014530.01.T01:CDS
GCAGAAGAAAGTGTTGAAGA+AGG 0.492469 3:+56134790 MsG0380014530.01.T01:CDS
GAGGGGCTAGAAGAAGAAAG+TGG 0.559399 3:+56134847 MsG0380014530.01.T01:CDS
AAGAAATGAAGAGGAAGACA+AGG 0.574706 3:+56134882 MsG0380014530.01.T01:CDS
CTATGTTAAAGAACCAATGT+TGG 0.600759 3:+56134684 MsG0380014530.01.T01:CDS
TGCATGTTCCTCATATGCAA+TGG 0.604080 3:+56134595 None:intergenic
AGGGGCTAGAAGAAGAAAGT+GGG 0.608592 3:+56134848 MsG0380014530.01.T01:CDS
ATTTGGTGAAAGAAATGAAG+AGG 0.618078 3:+56134873 MsG0380014530.01.T01:CDS
TGTTAAAGAACCAATGTTGG+TGG 0.649190 3:+56134687 MsG0380014530.01.T01:CDS
TATTGAAGATGAGTACACAA+TGG 0.672928 3:+56134744 MsG0380014530.01.T01:CDS
AAGTTTGGTGCTAAGCACTG+AGG 0.728832 3:+56134954 MsG0380014530.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTCTCTTCATTATTATT+AGG - Chr3:56134727-56134746 None:intergenic 15.0%
! AAAAGATGAAGAAAGATTTG+AGG + Chr3:56135007-56135026 MsG0380014530.01.T01:CDS 25.0%
! GAATTACAAGATCTTTATCT+TGG + Chr3:56134635-56134654 MsG0380014530.01.T01:CDS 25.0%
! TTCATTATTATTAGGCTCAT+TGG - Chr3:56134719-56134738 None:intergenic 25.0%
! TTGTAATTCCAACAAAAACA+AGG - Chr3:56134624-56134643 None:intergenic 25.0%
CTATGTTAAAGAACCAATGT+TGG + Chr3:56134684-56134703 MsG0380014530.01.T01:CDS 30.0%
TATTGAAGATGAGTACACAA+TGG + Chr3:56134744-56134763 MsG0380014530.01.T01:CDS 30.0%
! ATTTGGTGAAAGAAATGAAG+AGG + Chr3:56134873-56134892 MsG0380014530.01.T01:CDS 30.0%
!! CATTGGTTCTTTAACATAGT+TGG - Chr3:56134683-56134702 None:intergenic 30.0%
!!! AGTTTTAGAAGAAGCAAGTT+TGG + Chr3:56134939-56134958 MsG0380014530.01.T01:CDS 30.0%
AAGAAATGAAGAGGAAGACA+AGG + Chr3:56134882-56134901 MsG0380014530.01.T01:CDS 35.0%
AGGAAAAGGAAGTAGCATAT+TGG + Chr3:56134810-56134829 MsG0380014530.01.T01:CDS 35.0%
TGTTAAAGAACCAATGTTGG+TGG + Chr3:56134687-56134706 MsG0380014530.01.T01:CDS 35.0%
!! GAAAGTGTTGAAGAAGGAAA+AGG + Chr3:56134796-56134815 MsG0380014530.01.T01:CDS 35.0%
!!! AATGGTCTCCTTGTTTTTGT+TGG + Chr3:56134613-56134632 MsG0380014530.01.T01:CDS 35.0%
!!! AGGAAGTAGCATATTGGTTT+TGG + Chr3:56134816-56134835 MsG0380014530.01.T01:CDS 35.0%
!!! CATATTGGTTTTGGATCAAG+AGG + Chr3:56134825-56134844 MsG0380014530.01.T01:CDS 35.0%
!! GAAGAAGAAAGTGGGCTATT+TGG + Chr3:56134856-56134875 MsG0380014530.01.T01:CDS 40.0%
!! GCAGAAGAAAGTGTTGAAGA+AGG + Chr3:56134790-56134809 MsG0380014530.01.T01:CDS 40.0%
!! TTGGTTTTCACCACCAACAT+TGG - Chr3:56134700-56134719 None:intergenic 40.0%
!!! ATTGGTTTTGGATCAAGAGG+AGG + Chr3:56134828-56134847 MsG0380014530.01.T01:CDS 40.0%
!!! TTGGTTTTGGATCAAGAGGA+GGG + Chr3:56134829-56134848 MsG0380014530.01.T01:CDS 40.0%
AGGGGCTAGAAGAAGAAAGT+GGG + Chr3:56134848-56134867 MsG0380014530.01.T01:CDS 45.0%
CAAGGAGACCATTGCATATG+AGG - Chr3:56134606-56134625 None:intergenic 45.0%
GGAAGATTCATCAACGCTAC+TGG - Chr3:56134662-56134681 None:intergenic 45.0%
!! AAGTTTGGTGCTAAGCACTG+AGG + Chr3:56134954-56134973 MsG0380014530.01.T01:CDS 45.0%
!!! TGGTTTTGGATCAAGAGGAG+GGG + Chr3:56134830-56134849 MsG0380014530.01.T01:CDS 45.0%
GAGGGGCTAGAAGAAGAAAG+TGG + Chr3:56134847-56134866 MsG0380014530.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr3 gene 56134598 56135047 56134598 ID=MsG0380014530.01;Name=MsG0380014530.01
Chr3 mRNA 56134598 56135047 56134598 ID=MsG0380014530.01.T01;Parent=MsG0380014530.01;Name=MsG0380014530.01.T01;_AED=0.36;_eAED=0.36;_QI=0|-1|0|1|-1|1|1|0|149
Chr3 exon 56134598 56135047 56134598 ID=MsG0380014530.01.T01:exon:16139;Parent=MsG0380014530.01.T01
Chr3 CDS 56134598 56135047 56134598 ID=MsG0380014530.01.T01:cds;Parent=MsG0380014530.01.T01
Gene Sequence

>MsG0380014530.01.T01

ATGTTCCTCATATGCAATGGTCTCCTTGTTTTTGTTGGAATTACAAGATCTTTATCTTGGTCCAGTAGCGTTGATGAATCTTCCAACTATGTTAAAGAACCAATGTTGGTGGTGAAAACCAATGAGCCTAATAATAATGAAGAGAATATTGAAGATGAGTACACAATGGAAATCAAATACTCAAGTGTAAAAGCAGAAGAAAGTGTTGAAGAAGGAAAAGGAAGTAGCATATTGGTTTTGGATCAAGAGGAGGGGCTAGAAGAAGAAAGTGGGCTATTTGGTGAAAGAAATGAAGAGGAAGACAAGGAATCAAAAATAGTTGAAAGAGTTGAAGATGAAGAAGTTTTAGAAGAAGCAAGTTTGGTGCTAAGCACTGAGGAGTTGAACAAAAAATTTGATGACTTCATTAAAAAGATGAAGAAAGATTTGAGGATTGAAGCTCAAAAATAA

Protein sequence

>MsG0380014530.01.T01

MFLICNGLLVFVGITRSLSWSSSVDESSNYVKEPMLVVKTNEPNNNEENIEDEYTMEIKYSSVKAEESVEEGKGSSILVLDQEEGLEEESGLFGERNEEEDKESKIVERVEDEEVLEEASLVLSTEELNKKFDDFIKKMKKDLRIEAQK*