AlfalfaGEDB | MsG0480018586.01

M. sativa cultivar ZhongmuNo.1 / MsG0480018586.01

KEGG: -
GO: GO:0004484 GO:0005515 GO:0006370
PFAM: PF01331 PF01839

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018586.01.T01	XP_003604604.2	93.861	847	22	4	242	1082	51	873	0	1606

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018586.01.T01	F4IYM4	66.323	873	241	8	242	1082	44	895	0	1108

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018586.01.T01	G7JLW6	93.861	847	22	4	242	1082	51	873	0.0	1606

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048719.01	MsG0480018586.01	0.994511	7.411760e-208	7.997965e-201
MsG0180000065.01	MsG0480018586.01	0.804222	2.395446e-49	1.228213e-46
MsG0180000195.01	MsG0480018586.01	0.816831	4.515062e-52	3.233860e-49
MsG0180000353.01	MsG0480018586.01	0.812145	4.911236e-51	3.098154e-48
MsG0180002952.01	MsG0480018586.01	0.812573	3.961713e-51	2.527866e-48
MsG0180003690.01	MsG0480018586.01	0.807217	5.629106e-50	3.117405e-47
MsG0180003948.01	MsG0480018586.01	0.816551	5.216253e-52	3.707448e-49
MsG0180004320.01	MsG0480018586.01	0.805345	1.396406e-49	7.369937e-47
MsG0180006235.01	MsG0480018586.01	0.820449	6.818209e-53	5.396715e-50
MsG0280006425.01	MsG0480018586.01	0.809550	1.790060e-50	1.054160e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018586.01.T01	MTR_4g014960	93.861	847	22	4	242	1082	51	873	0.0	1606
MsG0480018586.01.T01	MTR_4g014920	63.851	296	33	5	14	241	395	684	1.16e-101	335
MsG0480018586.01.T01	MTR_4g014920	63.851	296	33	5	14	241	399	688	1.17e-101	336
MsG0480018586.01.T01	MTR_4g014920	68.137	204	18	2	14	176	399	596	7.28e-75	261
MsG0480018586.01.T01	MTR_7g066810	50.340	294	75	5	6	234	400	687	2.45e-72	254
MsG0480018586.01.T01	MTR_7g094670	47.213	305	84	8	14	249	344	640	1.12e-62	234
MsG0480018586.01.T01	MTR_4g014920	64.045	178	17	2	14	150	399	570	1.29e-58	213
MsG0480018586.01.T01	MTR_7g068260	70.492	61	18	0	121	181	53	113	7.38e-22	92.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018586.01.T01	AT3G09090	66.323	873	241	8	242	1082	44	895	0.0	1108
MsG0480018586.01.T01	AT3G09090	65.962	852	237	8	242	1061	44	874	0.0	1074
MsG0480018586.01.T01	AT3G09090	63.849	769	225	8	242	978	44	791	0.0	922
MsG0480018586.01.T01	AT3G09100	48.936	282	72	6	14	230	394	668	8.20e-66	236
MsG0480018586.01.T01	AT5G01290	45.070	284	82	7	14	230	377	653	2.36e-56	207
MsG0480018586.01.T01	AT5G28210	36.704	267	95	10	45	239	360	624	1.85e-32	135
MsG0480018586.01.T01	AT3G09100	52.703	74	29	1	14	87	394	461	5.49e-15	79.3

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Find 275 sgRNAs with CRISPR-Local

Find 1020 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGATGGCAATGGCACT+GGG	0.576438	4:-7024636	None:intergenic
AAACAGAAGGAAAAGAAGAA+GGG	0.505045	4:+7023675	MsG0480018586.01.T01:CDS
AAACTCTCACATGAGGCAGA+TGG	0.608691	4:+7011527	MsG0480018586.01.T01:CDS
AAAGCTCACTGCGGCACCCA+GGG	0.595695	4:-7024757	None:intergenic
AAAGTCAACTGAATTTAAAT+TGG	0.286326	4:-7011830	None:intergenic
AAATAGCACTTCACCATTGT+AGG	0.390827	4:-7019105	None:intergenic
AAATGGAAGCCAATCAGAGG+AGG	0.604090	4:+7019818	MsG0480018586.01.T01:CDS
AACAACACCTGCTTGAATCT+CGG	0.554490	4:-7022622	None:intergenic
AACCAGACCTATTATCAGCC+TGG	0.554997	4:+7024412	MsG0480018586.01.T01:CDS
AACCTTAAAATTTCAAGATC+TGG	0.365412	4:+7009891	MsG0480018586.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACAGAAGGAAAAGAAGAA+GGG	+	Chr4:7023675-7023694	MsG0480018586.01.T01:CDS	30.0%
AAATTAATACCACATCACGT+AGG	+	Chr4:7016310-7016329	MsG0480018586.01.T01:intron	30.0%
AACAAGGATTTGGAAGTAAA+AGG	-	Chr4:7011686-7011705	None:intergenic	30.0%
AACACGAATCGATAATATGA+CGG	-	Chr4:7018245-7018264	None:intergenic	30.0%
AACATCGTTATAAATGGCAT+GGG	-	Chr4:7014046-7014065	None:intergenic	30.0%
AAGAACACTAAGTTACTAAC+TGG	+	Chr4:7017955-7017974	MsG0480018586.01.T01:intron	30.0%
AAGATTGAATGCTCTATTCT+TGG	+	Chr4:7021449-7021468	MsG0480018586.01.T01:intron	30.0%
AATAAAACACCTCAAAGCAA+TGG	-	Chr4:7019182-7019201	None:intergenic	30.0%
AATAATGTTGCAAATCGGTA+CGG	+	Chr4:7024108-7024127	MsG0480018586.01.T01:CDS	30.0%
AATATGTATACAGCTGCAAA+GGG	-	Chr4:7020950-7020969	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	7009861	7025081	7009861	ID=MsG0480018586.01;Name=MsG0480018586.01
Chr4	mRNA	7009861	7025081	7009861	ID=MsG0480018586.01.T01;Parent=MsG0480018586.01;Name=MsG0480018586.01.T01;_AED=0.48;_eAED=0.52;_QI=0\|0.58\|0.72\|1\|1\|1\|18\|406\|1082
Chr4	exon	7024361	7025081	7024361	ID=MsG0480018586.01.T01:exon:2366;Parent=MsG0480018586.01.T01
Chr4	exon	7024081	7024263	7024081	ID=MsG0480018586.01.T01:exon:2365;Parent=MsG0480018586.01.T01
Chr4	exon	7023893	7024010	7023893	ID=MsG0480018586.01.T01:exon:2364;Parent=MsG0480018586.01.T01
Chr4	exon	7023507	7023790	7023507	ID=MsG0480018586.01.T01:exon:2363;Parent=MsG0480018586.01.T01
Chr4	exon	7022589	7022821	7022589	ID=MsG0480018586.01.T01:exon:2362;Parent=MsG0480018586.01.T01
Chr4	exon	7021763	7022019	7021763	ID=MsG0480018586.01.T01:exon:2361;Parent=MsG0480018586.01.T01
Chr4	exon	7020334	7020404	7020334	ID=MsG0480018586.01.T01:exon:2360;Parent=MsG0480018586.01.T01
Chr4	exon	7019622	7020220	7019622	ID=MsG0480018586.01.T01:exon:2359;Parent=MsG0480018586.01.T01
Chr4	exon	7019232	7019392	7019232	ID=MsG0480018586.01.T01:exon:2358;Parent=MsG0480018586.01.T01
Chr4	exon	7018938	7019132	7018938	ID=MsG0480018586.01.T01:exon:2357;Parent=MsG0480018586.01.T01
Chr4	exon	7017120	7017230	7017120	ID=MsG0480018586.01.T01:exon:2356;Parent=MsG0480018586.01.T01
Chr4	exon	7013055	7013197	7013055	ID=MsG0480018586.01.T01:exon:2355;Parent=MsG0480018586.01.T01
Chr4	exon	7012654	7012703	7012654	ID=MsG0480018586.01.T01:exon:2354;Parent=MsG0480018586.01.T01
Chr4	exon	7011968	7012049	7011968	ID=MsG0480018586.01.T01:exon:2353;Parent=MsG0480018586.01.T01
Chr4	exon	7011772	7011861	7011772	ID=MsG0480018586.01.T01:exon:2352;Parent=MsG0480018586.01.T01
Chr4	exon	7011464	7011562	7011464	ID=MsG0480018586.01.T01:exon:2351;Parent=MsG0480018586.01.T01
Chr4	exon	7010831	7010968	7010831	ID=MsG0480018586.01.T01:exon:2350;Parent=MsG0480018586.01.T01
Chr4	exon	7009861	7009980	7009861	ID=MsG0480018586.01.T01:exon:2349;Parent=MsG0480018586.01.T01
Chr4	CDS	7009861	7009980	7009861	ID=MsG0480018586.01.T01:cds;Parent=MsG0480018586.01.T01
Chr4	CDS	7010831	7010968	7010831	ID=MsG0480018586.01.T01:cds;Parent=MsG0480018586.01.T01
Chr4	CDS	7011464	7011562	7011464	ID=MsG0480018586.01.T01:cds;Parent=MsG0480018586.01.T01
Chr4	CDS	7011772	7011861	7011772	ID=MsG0480018586.01.T01:cds;Parent=MsG0480018586.01.T01
Chr4	CDS	7011968	7012049	7011968	ID=MsG0480018586.01.T01:cds;Parent=MsG0480018586.01.T01
Chr4	CDS	7012654	7012703	7012654	ID=MsG0480018586.01.T01:cds;Parent=MsG0480018586.01.T01
Chr4	CDS	7013055	7013197	7013055	ID=MsG0480018586.01.T01:cds;Parent=MsG0480018586.01.T01
Chr4	CDS	7017120	7017230	7017120	ID=MsG0480018586.01.T01:cds;Parent=MsG0480018586.01.T01
Chr4	CDS	7018938	7019132	7018938	ID=MsG0480018586.01.T01:cds;Parent=MsG0480018586.01.T01
Chr4	CDS	7019232	7019392	7019232	ID=MsG0480018586.01.T01:cds;Parent=MsG0480018586.01.T01
Chr4	CDS	7019622	7020220	7019622	ID=MsG0480018586.01.T01:cds;Parent=MsG0480018586.01.T01
Chr4	CDS	7020334	7020404	7020334	ID=MsG0480018586.01.T01:cds;Parent=MsG0480018586.01.T01
Chr4	CDS	7021763	7022019	7021763	ID=MsG0480018586.01.T01:cds;Parent=MsG0480018586.01.T01
Chr4	CDS	7022589	7022821	7022589	ID=MsG0480018586.01.T01:cds;Parent=MsG0480018586.01.T01
Chr4	CDS	7023507	7023790	7023507	ID=MsG0480018586.01.T01:cds;Parent=MsG0480018586.01.T01
Chr4	CDS	7023893	7024010	7023893	ID=MsG0480018586.01.T01:cds;Parent=MsG0480018586.01.T01
Chr4	CDS	7024081	7024263	7024081	ID=MsG0480018586.01.T01:cds;Parent=MsG0480018586.01.T01
Chr4	CDS	7024361	7024675	7024361	ID=MsG0480018586.01.T01:cds;Parent=MsG0480018586.01.T01
Chr4	three_prime_UTR	7024676	7025081	7024676	ID=MsG0480018586.01.T01:three_prime_utr;Parent=MsG0480018586.01.T01

Gene Sequence

>MsG0480018586.01.T01

ATGGTTTTCAGCACTGTTCTATTTAATGCCAACCTTAAAATTTCAAGATCTGGCCAAATCACCCAAGTATCCTTGGTGTTCCTAGACTGGCCAGTGGCACCTAGTGTCCAGTATTCATCGGGTTTGGGCGAGAAGACCCACCATTTCACATTGCTGGATGGTGAGATGGTTATCGATATCTTGCCAGATTCAAATAAGCAGGAGAGAAGATACCTAATATATGATCTTATGGCAATCAACCATGTTTCAGTAATAGAGGTAAGGAGGAAAGATTTTTGGTTGCTCTCTACTGTCACAAAGCTTTTGAAGGAATTCATTAAAAAACTCTCACATGAGGCAGATGGTCTTATATTTCAGGGTTGGGACGATCCTTATGTACCACGTACTCATGAAGGACTCTTAAAGTGGAAATACGCCAATTTAAATTCAGTTGACTTTCTGTTTGAGGTTGAAGGTGATCGGGAGCTACTTTTTGTGTATGAGTGGGGAAAGAAGAAACTCTTGGATGGGAATAAGGTTGCTTTCAAAGTTTCTAATTTAGTGCCCACCAAAAAGTGGGTTGAGAAGGATGATGCTTTAGTGATGCAAAGCATAAAAGACAACATCACAGAGGAAGACTTGTTGAATGAAATAAATGAGATAATTCGCCTTCCCATGTATGCGGACAGGATAAAAAGTGATAGTAAAGCAAATCAGATTGCTCATGCCGCAAAGCAGCGAAGAGATGAAGATGCTTTGGTGAATTCGAAATGTCCTGTGAATTTAGAGCTAAGATGGCAAACGGAAGTTAGTTCTAGTGTTTATGCAAATCCCTTGATTGCTGATATTAACAGCACCTGTCCTTATTCTGATAGATCATCTGGTGTTCGATTTGACTTTATTCAAATCATTCTTCTTCTTGGTTTTTTAGGCTGGCCTGCTTTCCACCAATCGACTGTGCATTCTAGTCCACTTCTATATGACATAGACAAAGATGGCGTGAGAGAAATAGCTTTGGCAACCTACAATGGTGAAGTGCTATTTTTCAGGGTTTCCGGCTATATCATGTCAGACAAGCTTGAGGTCCCACGTAGGAAAGTGCTTAAAAATTGGTATGTGGGTTTGAATAAGGATCCAGTGGACCGTACCCATCCTGATGTTCATGATGACCAACTTGTCCAAGAAGCTACTATCGCGAATTCTATGTCCCAAATGAATGGAAGCCGACACGAAGTTAATTCCTCAGCTTCCACATCAACAGAAAGTCACCCTGACACAAAAAGCGTGTCTAATCCAGAGCCTGAAAAGAAAATAAATGGAAGCCAATCAGAGGAGAGTATTAACACATCAACAGAAAGTCACCCTGATACAAAAAATGTGTCTAATCCAGAGCCTGAAAAGAAAGTAAATGGAAGCCAATCAGAGGAGGGTATTAAGATGCCAACAGTTGCTGATAATTCATCTGTCAGTGCTGGTTCTGTGGAGACTGTTAATGCAGACAACAAGACTAGCATTGGGAGGCGGCTGCTGGAAGATAACAACTTAAAAGGAGCTGAGCAAGTTGGTTCTGAATCCAAAGGTAAGGAAGAAGTTCATGCTGCAACTGTGGAAAATGAGGAAGGACTGGAAGCAGATGCTGATTCATCTTTTGAGTTATTCCGCAATAGTGATGACCTGGCTGATGAGTACAACTATGATTATGATGATTATGTTGATGAATCATTGTGGGGTGATGAAGAATGGATTGAAGGGAAGCATGAGAAATTAGAGGATTACGTGAATGTTGACTCACACATCTTGTCCACTCCTGTCATTGCAGATATTGACAATGATGGCGTAATGGAAATGGTTGTTGCTGTTTCTTATTTTTTTGACCAAGAGTATTATGACAACCAGGAGCATATGAAAGAATTGGGCGATATTGACATTGGGAAATATGTTGCTGGTGGTATTGTTGTTTTTAATCTGGACACAAAACAAGTCAAGTGGACTGCAGAACTTGATATGAGTACAGATACAGCAAACTTCCGAGCGTACGTATATTCTTCTCCAACTGTTGTAGACTTGGATGGTGATGGGTATCTGGACATTCTTGTTGGAACTTCTTATGGCCTATTCTATGTGTTAGATCATCATGGTAAAGTTAGAGAGAAGTTCCCTCTTGAAATGGCCGAGATTCAAGCAGGTGTTGTTGCAGCTGATATTAATGATGATGGAAAAATTGAGCTAGTTACTGCTGATACCCATGGAAATGTTGTTGCATGGACCCCCAAAGGAGATATGATTTGGGAAAAGCATCTGAAGAGTCTTATTCCACATGTAATGTACTATCTCAATCTTCCTTGGCATGTCAATGAGTGTAGCATGATTGCTCCAACTATTGGTGATATCGACGGGGATGGCCGTACTGAGCTTGTTGTACCAACACTATCTGGGAAGATTCATGTTCTTGATGGAAGGGATGGATCACCTATTGGACGGTACCCATTTATAACTCATGGAAGAATAATGAATCAAATTCTTCTTGTTGATTTAAGTAAACAGAAGGAAAAGAAGAAGGGATTGACCCTTGTTACCTCTTCGTTTGATGGTTATTTGTACCTCATTGATGGACCTACAGGCTGTGCTGATGTTGTTGACATTGGTGAAACTTCATATAGCATGGTCTTGGCAGACAATGTAGATGGTGGGGATGATCTTGATCTGATTGTCTCGACAATGAATGGCAACGTCTTCTGCTTCTCGACTCCCTCACCTCACCATCCCCTCAAGGCATGGAGATTGCCTAACCAAGGAAGAAATAATGTTGCAAATCGGTACGGTCGTGAAGGCATCTATGTAACTCATCCTTCAAGGGCTTTCCGTGATGAGGAAGGAAAGAGTTTTTTTGTGGAAATTGAGATTGTAGATAATTATAGATACCCATCTGGTCATCAAGGACCATACCATGTCACTACTTCCTTGCTTGTCCCTGGCAATTACCAAGGAGAGAGAACAATAAAGCAGAACCAGACCTATTATCAGCCTGGTAAACATCGAATTAAGCTGCCAACTGTGGGAGTAAGGACCACAGGAACAGTTTTGGTCGAGATGGTTGACAAAAATGGACTCTATTTCTCTGATGAATTCTCTCTTACATTCCATATGCACTACTATAAATTGTTGAAGTGGCTTCTTGTTCTTCCAATGCTTGGGATGTTTGGTGTGCTTGTCATCCTTCGCCCACAAGGCCCAGTGCCATTGCCATCATTTTCAAGGAACAACGATTAG

Protein sequence

>MsG0480018586.01.T01

MVFSTVLFNANLKISRSGQITQVSLVFLDWPVAPSVQYSSGLGEKTHHFTLLDGEMVIDILPDSNKQERRYLIYDLMAINHVSVIEVRRKDFWLLSTVTKLLKEFIKKLSHEADGLIFQGWDDPYVPRTHEGLLKWKYANLNSVDFLFEVEGDRELLFVYEWGKKKLLDGNKVAFKVSNLVPTKKWVEKDDALVMQSIKDNITEEDLLNEINEIIRLPMYADRIKSDSKANQIAHAAKQRRDEDALVNSKCPVNLELRWQTEVSSSVYANPLIADINSTCPYSDRSSGVRFDFIQIILLLGFLGWPAFHQSTVHSSPLLYDIDKDGVREIALATYNGEVLFFRVSGYIMSDKLEVPRRKVLKNWYVGLNKDPVDRTHPDVHDDQLVQEATIANSMSQMNGSRHEVNSSASTSTESHPDTKSVSNPEPEKKINGSQSEESINTSTESHPDTKNVSNPEPEKKVNGSQSEEGIKMPTVADNSSVSAGSVETVNADNKTSIGRRLLEDNNLKGAEQVGSESKGKEEVHAATVENEEGLEADADSSFELFRNSDDLADEYNYDYDDYVDESLWGDEEWIEGKHEKLEDYVNVDSHILSTPVIADIDNDGVMEMVVAVSYFFDQEYYDNQEHMKELGDIDIGKYVAGGIVVFNLDTKQVKWTAELDMSTDTANFRAYVYSSPTVVDLDGDGYLDILVGTSYGLFYVLDHHGKVREKFPLEMAEIQAGVVAADINDDGKIELVTADTHGNVVAWTPKGDMIWEKHLKSLIPHVMYYLNLPWHVNECSMIAPTIGDIDGDGRTELVVPTLSGKIHVLDGRDGSPIGRYPFITHGRIMNQILLVDLSKQKEKKKGLTLVTSSFDGYLYLIDGPTGCADVVDIGETSYSMVLADNVDGGDDLDLIVSTMNGNVFCFSTPSPHHPLKAWRLPNQGRNNVANRYGREGIYVTHPSRAFRDEEGKSFFVEIEIVDNYRYPSGHQGPYHVTTSLLVPGNYQGERTIKQNQTYYQPGKHRIKLPTVGVRTTGTVLVEMVDKNGLYFSDEFSLTFHMHYYKLLKWLLVLPMLGMFGVLVILRPQGPVPLPSFSRNND*