Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018659.01.T01 | XP_024637738.1 | 81.472 | 394 | 39 | 3 | 118 | 477 | 1 | 394 | 0 | 602 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047869.01 | MsG0480018659.01 | 0.841729 | 3.981661e-58 | 5.903370e-55 |
| MsG0080047957.01 | MsG0480018659.01 | 0.800160 | 1.641491e-48 | 7.589204e-46 |
| MsG0080047959.01 | MsG0480018659.01 | 0.803553 | 3.299000e-49 | 1.662834e-46 |
| MsG0080048461.01 | MsG0480018659.01 | 0.805979 | 1.027692e-49 | 5.513275e-47 |
| MsG0080048804.01 | MsG0480018659.01 | 0.821005 | 5.081725e-53 | 4.085260e-50 |
| MsG0080048874.01 | MsG0480018659.01 | 0.809364 | 1.962451e-50 | 1.149991e-47 |
| MsG0080048964.01 | MsG0480018659.01 | 0.865705 | 4.863422e-65 | 1.597465e-61 |
| MsG0080049032.01 | MsG0480018659.01 | 0.819621 | 1.055183e-52 | 8.160991e-50 |
| MsG0080049058.01 | MsG0480018659.01 | 0.808272 | 3.359283e-50 | 1.912541e-47 |
| MsG0180000065.01 | MsG0480018659.01 | 0.849583 | 2.946010e-60 | 5.610872e-57 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018659.01.T01 | MTR_4g016590 | 81.472 | 394 | 39 | 3 | 118 | 477 | 1 | 394 | 0.0 | 602 |
| MsG0480018659.01.T01 | MTR_8g040870 | 52.174 | 115 | 34 | 1 | 22 | 115 | 38 | 152 | 1.82e-33 | 126 |
| MsG0480018659.01.T01 | MTR_1g040935 | 44.366 | 142 | 47 | 1 | 2 | 111 | 1 | 142 | 2.08e-32 | 127 |
| MsG0480018659.01.T01 | MTR_0195s0010 | 46.094 | 128 | 37 | 1 | 1 | 96 | 122 | 249 | 2.69e-32 | 124 |
| MsG0480018659.01.T01 | MTR_0195s0020 | 46.512 | 129 | 37 | 1 | 1 | 97 | 122 | 250 | 4.17e-32 | 129 |
| MsG0480018659.01.T01 | MTR_6g045237 | 42.177 | 147 | 53 | 1 | 1 | 115 | 115 | 261 | 1.47e-31 | 127 |
| MsG0480018659.01.T01 | MTR_4g031410 | 47.059 | 119 | 59 | 1 | 1 | 115 | 9 | 127 | 5.09e-30 | 118 |
| MsG0480018659.01.T01 | MTR_4g031755 | 47.059 | 119 | 59 | 1 | 1 | 115 | 9 | 127 | 5.09e-30 | 118 |
| MsG0480018659.01.T01 | MTR_4g031785 | 33.838 | 198 | 113 | 5 | 1 | 180 | 9 | 206 | 1.57e-29 | 119 |
| MsG0480018659.01.T01 | MTR_1g041005 | 42.336 | 137 | 47 | 1 | 3 | 107 | 59 | 195 | 3.71e-29 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018659.01.T01 | AT3G17712 | 42.359 | 373 | 169 | 8 | 141 | 475 | 18 | 382 | 2.96e-66 | 228 |
| MsG0480018659.01.T01 | AT3G17740 | 42.359 | 373 | 169 | 8 | 141 | 475 | 18 | 382 | 1.05e-64 | 227 |
| MsG0480018659.01.T01 | AT3G17712 | 40.230 | 348 | 162 | 8 | 141 | 450 | 18 | 357 | 1.00e-52 | 190 |
| MsG0480018659.01.T01 | AT3G17712 | 40.230 | 348 | 162 | 8 | 141 | 450 | 49 | 388 | 2.77e-52 | 190 |
Find 200 sgRNAs with CRISPR-Local
Find 669 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGAGGCTCGACTATATT+TGG | 0.284322 | 4:+8171260 | MsG0480018659.01.T01:three_prime_UTR |
| AAAATACGTCGAGCTGCAGA+AGG | 0.595640 | 4:-8175901 | None:intergenic |
| AAAATACTCTTGCAACATTC+TGG | 0.251489 | 4:+8169819 | MsG0480018659.01.T01:CDS |
| AAACTGAACTCTGTTCTTAC+TGG | 0.335846 | 4:+8176130 | MsG0480018659.01.T01:three_prime_UTR |
| AAATGACCAATCTGTCGCTC+TGG | 0.499183 | 4:+8173728 | MsG0480018659.01.T01:three_prime_UTR |
| AACATACCAGAGCGACAGAT+TGG | 0.504775 | 4:-8173734 | None:intergenic |
| AACCAAACTATTTCATCATG+CGG | 0.599477 | 4:-8169600 | None:intergenic |
| AACCATGGAAACCTTGAACT+TGG | 0.502602 | 4:-8169891 | None:intergenic |
| AACCGCATGATGAAATAGTT+TGG | 0.410461 | 4:+8169598 | MsG0480018659.01.T01:CDS |
| AACTCATCTTGTGTTGTATG+AGG | 0.482210 | 4:-8169438 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAAGTTCACTCGACAATT+GGG | + | Chr4:8174370-8174389 | MsG0480018659.01.T01:intron | 30.0% | |
| AAAATACTCTTGCAACATTC+TGG | + | Chr4:8169819-8169838 | MsG0480018659.01.T01:CDS | 30.0% | |
| AAAATGAATTCTTCTAACCG+TGG | + | Chr4:8172289-8172308 | MsG0480018659.01.T01:intron | 30.0% | |
| AAATGAAAAGCTGATCAAAG+AGG | + | Chr4:8173005-8173024 | MsG0480018659.01.T01:intron | 30.0% | |
| AAATGAATTCTTCTAACCGT+GGG | + | Chr4:8172290-8172309 | MsG0480018659.01.T01:intron | 30.0% | |
| AACCAAACTATTTCATCATG+CGG | - | Chr4:8169603-8169622 | None:intergenic | 30.0% | |
| AACTTCTCTAACATAGAATC+TGG | - | Chr4:8172070-8172089 | None:intergenic | 30.0% | |
| AAGATTAATGCATGAGAGAA+AGG | - | Chr4:8175465-8175484 | None:intergenic | 30.0% | |
| AATACTTGGGTGAAATAGAT+AGG | + | Chr4:8174028-8174047 | MsG0480018659.01.T01:intron | 30.0% | |
| AATATAATGACGATAGAGCT+AGG | + | Chr4:8168714-8168733 | MsG0480018659.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 8166229 | 8176654 | 8166229 | ID=MsG0480018659.01;Name=MsG0480018659.01 |
| Chr4 | mRNA | 8166229 | 8176654 | 8166229 | ID=MsG0480018659.01.T01;Parent=MsG0480018659.01;Name=MsG0480018659.01.T01;_AED=0.32;_eAED=0.48;_QI=0|0.27|0.25|1|1|1|12|1857|484 |
| Chr4 | exon | 8166229 | 8166327 | 8166229 | ID=MsG0480018659.01.T01:exon:2636;Parent=MsG0480018659.01.T01 |
| Chr4 | exon | 8166424 | 8166666 | 8166424 | ID=MsG0480018659.01.T01:exon:2637;Parent=MsG0480018659.01.T01 |
| Chr4 | exon | 8167371 | 8167873 | 8167371 | ID=MsG0480018659.01.T01:exon:2638;Parent=MsG0480018659.01.T01 |
| Chr4 | exon | 8169210 | 8169480 | 8169210 | ID=MsG0480018659.01.T01:exon:2639;Parent=MsG0480018659.01.T01 |
| Chr4 | exon | 8169573 | 8169910 | 8169573 | ID=MsG0480018659.01.T01:exon:2640;Parent=MsG0480018659.01.T01 |
| Chr4 | exon | 8170467 | 8170732 | 8170467 | ID=MsG0480018659.01.T01:exon:2641;Parent=MsG0480018659.01.T01 |
| Chr4 | exon | 8171154 | 8171325 | 8171154 | ID=MsG0480018659.01.T01:exon:2642;Parent=MsG0480018659.01.T01 |
| Chr4 | exon | 8172012 | 8172307 | 8172012 | ID=MsG0480018659.01.T01:exon:2643;Parent=MsG0480018659.01.T01 |
| Chr4 | exon | 8173184 | 8173296 | 8173184 | ID=MsG0480018659.01.T01:exon:2644;Parent=MsG0480018659.01.T01 |
| Chr4 | exon | 8173489 | 8173840 | 8173489 | ID=MsG0480018659.01.T01:exon:2645;Parent=MsG0480018659.01.T01 |
| Chr4 | exon | 8175848 | 8175950 | 8175848 | ID=MsG0480018659.01.T01:exon:2646;Parent=MsG0480018659.01.T01 |
| Chr4 | exon | 8176099 | 8176654 | 8176099 | ID=MsG0480018659.01.T01:exon:2647;Parent=MsG0480018659.01.T01 |
| Chr4 | CDS | 8166229 | 8166327 | 8166229 | ID=MsG0480018659.01.T01:cds;Parent=MsG0480018659.01.T01 |
| Chr4 | CDS | 8166424 | 8166666 | 8166424 | ID=MsG0480018659.01.T01:cds;Parent=MsG0480018659.01.T01 |
| Chr4 | CDS | 8167371 | 8167873 | 8167371 | ID=MsG0480018659.01.T01:cds;Parent=MsG0480018659.01.T01 |
| Chr4 | CDS | 8169210 | 8169480 | 8169210 | ID=MsG0480018659.01.T01:cds;Parent=MsG0480018659.01.T01 |
| Chr4 | CDS | 8169573 | 8169910 | 8169573 | ID=MsG0480018659.01.T01:cds;Parent=MsG0480018659.01.T01 |
| Chr4 | CDS | 8170467 | 8170467 | 8170467 | ID=MsG0480018659.01.T01:cds;Parent=MsG0480018659.01.T01 |
| Chr4 | three_prime_UTR | 8170468 | 8170732 | 8170468 | ID=MsG0480018659.01.T01:three_prime_utr;Parent=MsG0480018659.01.T01 |
| Chr4 | three_prime_UTR | 8171154 | 8171325 | 8171154 | ID=MsG0480018659.01.T01:three_prime_utr;Parent=MsG0480018659.01.T01 |
| Chr4 | three_prime_UTR | 8172012 | 8172307 | 8172012 | ID=MsG0480018659.01.T01:three_prime_utr;Parent=MsG0480018659.01.T01 |
| Chr4 | three_prime_UTR | 8173184 | 8173296 | 8173184 | ID=MsG0480018659.01.T01:three_prime_utr;Parent=MsG0480018659.01.T01 |
| Chr4 | three_prime_UTR | 8173489 | 8173840 | 8173489 | ID=MsG0480018659.01.T01:three_prime_utr;Parent=MsG0480018659.01.T01 |
| Chr4 | three_prime_UTR | 8175848 | 8175950 | 8175848 | ID=MsG0480018659.01.T01:three_prime_utr;Parent=MsG0480018659.01.T01 |
| Chr4 | three_prime_UTR | 8176099 | 8176654 | 8176099 | ID=MsG0480018659.01.T01:three_prime_utr;Parent=MsG0480018659.01.T01 |
| Gene Sequence |
>MsG0480018659.01.T01 ATGCTTTCGTTGGGGCGTGAGTATTATGAGTTCTCGTTTGCCACCTATGAAGACTTGCGAGCAGCATGGGCATTAGGCACAATCAATTTGAAGTCGGGTGAATATTGGATAGAGAGAACCTTGTATGAAATAGTGGGGTCTATCGAGACTCCGTTGTTAATTGATAATCTTACTAAGAACATGTTGTTTGGTCATTATGCCAAGGTTTTGTTGGACTTAGACCTTTCTAGGAACATTTTTATTGAAGTTATGGTGGAACATGAAGGTTATGCATTTCCGGTGGAAATTGAATATGATGGGTTGCCTGATTTTTGCACTCACTGTAAACGTATTAGACATAATATTTTCAAAATGGACCAAAAACCACCTCATTCGGCGGAAGAAACGACGGCGCCACCGTCCGACGAAGCAAAACCTTCACTCTTCTCTGTCTTCCCCGTAACAAAATCTTCTCTTCAAATCACAACTTCTTCGCTTCCTCAATGGCTCAGTAACTCTAGCTTCACAACCGACATCGCCGTCATCAATGACGATGTCGCTTCATTACTCAACCGCGAAACCGTGCAATCGCCGCCACCAGACGACGATGAAAACAGCGATGAAAATCGACCTAAGGAGAAGTCTTACGCGATTCTCGAATCTTCGGAATCGGATGGAGATGGAATGAAGAAAGAGAAGAAGAGGAAGAAGAAGCGGAAGCGTGATAGGTCTGATGAGAGAAGTGGATTTGGTTCGAGGAAATCGCGTGTTCGAGCTTGGGCTGATTCTGAGGCTAATACTGTTAAAGATTATTACTTTGATTCTCATGGTGATCGTGATAATCTTGCATTTGGCTGTATCTATAGAATGGATATTGCCCGGCACAAACCGTATAATCCCTTGAATATGTCTGGGCGACATGTTAAAGGTTTGTATTGGTGGAATCAAAGTGGTTCACTTGGGGAAAGAGATGGCGATATTGATGCTTTGGATGATAAAATGAAGTCTGCTGGGCGATACTGGTCCGGAAAGTATATGGCTTTAGAACGACATAAAAGCTTCAAGCGCCTACGTCTTGTTGCTCCAAAATGTCTCCTCATACAACACAAGATGAGTTTATACCTTTATCAGATGTTGGGAGTTCAACAAACTGACAAGGGAGAACCGCATGATGAAATAGTTTGGTTACATTTTGCCGAGTTCCAAGATAAGGTTGCAGGAATGCAACGGCAGAAAGGTGCTCGCTTGCAAATACTTGAAAAGAAAATTAGCATTCTGGAGAAGGCAGTTGAGCTTAACCCAGAGAATGAAAATCTATTACTTTGCCTTTTAAAAGCTTATCAAACAAGAGATAGCTCAGATGTGTTAATTGGGAGATGGGAGAAAATACTCTTGCAACATTCTGGAAGTTACAAGTTATGGAGTGAATTCTTGCATGTTGTTCAGAGGAATTCTCCAAGTTCAAGGTTTCCATGA |
| Protein sequence |
>MsG0480018659.01.T01 MLSLGREYYEFSFATYEDLRAAWALGTINLKSGEYWIERTLYEIVGSIETPLLIDNLTKNMLFGHYAKVLLDLDLSRNIFIEVMVEHEGYAFPVEIEYDGLPDFCTHCKRIRHNIFKMDQKPPHSAEETTAPPSDEAKPSLFSVFPVTKSSLQITTSSLPQWLSNSSFTTDIAVINDDVASLLNRETVQSPPPDDDENSDENRPKEKSYAILESSESDGDGMKKEKKRKKKRKRDRSDERSGFGSRKSRVRAWADSEANTVKDYYFDSHGDRDNLAFGCIYRMDIARHKPYNPLNMSGRHVKGLYWWNQSGSLGERDGDIDALDDKMKSAGRYWSGKYMALERHKSFKRLRLVAPKCLLIQHKMSLYLYQMLGVQQTDKGEPHDEIVWLHFAEFQDKVAGMQRQKGARLQILEKKISILEKAVELNPENENLLLCLLKAYQTRDSSDVLIGRWEKILLQHSGSYKLWSEFLHVVQRNSPSSRFP* |
