AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047763.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047763.01.T01 MTR_4g134340 98.795 83 1 0 1 83 349 431 1.46e-57 182
MsG0880047763.01.T01 MTR_4g134340 98.795 83 1 0 1 83 356 438 1.47e-57 182
MsG0880047763.01.T01 MTR_1g043000 97.619 84 1 1 1 83 364 447 2.71e-55 176
MsG0880047763.01.T01 MTR_8g042940 86.747 83 11 0 1 83 341 423 1.14e-48 159
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047763.01.T01 AT1G28560 59.036 83 31 3 1 83 307 386 3.00e-24 94.0
MsG0880047763.01.T01 AT1G28560 59.036 83 31 3 1 83 296 375 3.48e-24 93.6
MsG0880047763.01.T01 AT1G28560 59.036 83 31 3 1 83 383 462 4.20e-24 94.0

Find 15 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCCGATGATGTACAGAAT+TGG 0.370973 8:-90527781 MsG0880047763.01.T01:CDS
TTCAGTTGAAAAGTGACAAT+TGG 0.371285 8:+90527749 None:intergenic
CATCCTCTGCTAGGTGAAGA+AGG 0.470985 8:+90527615 None:intergenic
AGGTCACTGTGGATGACAAA+TGG 0.479331 8:-90527679 MsG0880047763.01.T01:CDS
CTTCTTCACCTAGCAGAGGA+TGG 0.493676 8:-90527614 MsG0880047763.01.T01:CDS
TGCCGATGATGTACAGAATT+GGG 0.506290 8:-90527780 MsG0880047763.01.T01:CDS
TTCCCTTCTTCACCTAGCAG+AGG 0.521159 8:-90527618 MsG0880047763.01.T01:CDS
AGACTAACAACAATTAATCA+TGG 0.540971 8:+90527552 None:intergenic
CAAAATATTCAGAGCTACAA+AGG 0.552654 8:-90527699 MsG0880047763.01.T01:CDS
ATGGAGAGCCATCCTCTGCT+AGG 0.564427 8:+90527606 None:intergenic
AGCCCAATTCTGTACATCAT+CGG 0.585350 8:+90527778 None:intergenic
ATAATCATATTCTATGAAGT+CGG 0.597290 8:+90527577 None:intergenic
ATCCTCTGCTAGGTGAAGAA+GGG 0.601005 8:+90527616 None:intergenic
TCTATGAAGTCGGTATACGA+TGG 0.646588 8:+90527587 None:intergenic
CAGAGCTACAAAGGTCACTG+TGG 0.721614 8:-90527690 MsG0880047763.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATCATATTCTATGAAGT+CGG + Chr8:90527785-90527804 None:intergenic 20.0%
!! ATTCATCACAAAAATAACAA+GGG + Chr8:90527717-90527736 None:intergenic 20.0%
! CATTCATCACAAAAATAACA+AGG + Chr8:90527718-90527737 None:intergenic 25.0%
CAAAATATTCAGAGCTACAA+AGG - Chr8:90527660-90527679 MsG0880047763.01.T01:CDS 30.0%
TTCAGTTGAAAAGTGACAAT+TGG + Chr8:90527613-90527632 None:intergenic 30.0%
! AAGATACGTTTTCAGAAATG+CGG - Chr8:90527631-90527650 MsG0880047763.01.T01:CDS 30.0%
CAAAAATAACAAGGGTTGTC+TGG + Chr8:90527709-90527728 None:intergenic 35.0%
AGCCCAATTCTGTACATCAT+CGG + Chr8:90527584-90527603 None:intergenic 40.0%
ATGCCGATGATGTACAGAAT+TGG - Chr8:90527578-90527597 MsG0880047763.01.T01:CDS 40.0%
TCTATGAAGTCGGTATACGA+TGG + Chr8:90527775-90527794 None:intergenic 40.0%
TGCCGATGATGTACAGAATT+GGG - Chr8:90527579-90527598 MsG0880047763.01.T01:CDS 40.0%
AGGTCACTGTGGATGACAAA+TGG - Chr8:90527680-90527699 MsG0880047763.01.T01:CDS 45.0%
ATCCTCTGCTAGGTGAAGAA+GGG + Chr8:90527746-90527765 None:intergenic 45.0%
CAGAGCTACAAAGGTCACTG+TGG - Chr8:90527669-90527688 MsG0880047763.01.T01:CDS 50.0%
CATCCTCTGCTAGGTGAAGA+AGG + Chr8:90527747-90527766 None:intergenic 50.0%
CTTCTTCACCTAGCAGAGGA+TGG - Chr8:90527745-90527764 MsG0880047763.01.T01:CDS 50.0%
TTCCCTTCTTCACCTAGCAG+AGG - Chr8:90527741-90527760 MsG0880047763.01.T01:CDS 50.0%
ATGGAGAGCCATCCTCTGCT+AGG + Chr8:90527756-90527775 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 90527565 90527816 90527565 ID=MsG0880047763.01;Name=MsG0880047763.01
Chr8 mRNA 90527565 90527816 90527565 ID=MsG0880047763.01.T01;Parent=MsG0880047763.01;Name=MsG0880047763.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|83
Chr8 exon 90527565 90527816 90527565 ID=MsG0880047763.01.T01:exon:15871;Parent=MsG0880047763.01.T01
Chr8 CDS 90527565 90527816 90527565 ID=MsG0880047763.01.T01:cds;Parent=MsG0880047763.01.T01
Gene Sequence

>MsG0880047763.01.T01

ATGAGGTTAATTCATGCCGATGATGTACAGAATTGGGCTGTTTATCCAATTGTCACTTTTCAACTGAAGATACGTTTTCAGAAATGCGGTGTTTGCAAAATATTCAGAGCTACAAAGGTCACTGTGGATGACAAATGGACACCAGACAACCCTTGTTATTTTTGTGATGAATGTTTTTCCCTTCTTCACCTAGCAGAGGATGGCTCTCCATCGTATACCGACTTCATAGAATATGATTATAACCATGATTAA

Protein sequence

>MsG0880047763.01.T01

MRLIHADDVQNWAVYPIVTFQLKIRFQKCGVCKIFRATKVTVDDKWTPDNPCYFCDECFSLLHLAEDGSPSYTDFIEYDYNHD*