AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280008660.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008660.01.T01 MTR_2g047838 75.000 60 14 1 10 69 1 59 4.72e-25 91.3
MsG0280008660.01.T01 MTR_8g089310 69.767 43 13 0 3 45 7 49 1.19e-14 65.1
MsG0280008660.01.T01 MTR_8g089310 71.053 38 11 0 8 45 46 83 3.82e-13 61.6
MsG0280008660.01.T01 MTR_8g089310 69.444 36 11 0 10 45 1 36 4.80e-12 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 109 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGGAAGAACAGGAATAAC+AGG 0.354231 2:-36016925 None:intergenic
GTTATTCCTGTTCTTCCTCC+TGG 0.387766 2:+36016928 MsG0280008660.01.T01:CDS
TCCATTCCTGCAGGCACATC+AGG 0.426300 2:-36017971 None:intergenic
CGGAGCCACATTATGTGTAC+TGG 0.429482 2:+36016832 MsG0280008660.01.T01:CDS
AAGATACCAGGAGGAAGAAC+AGG 0.433787 2:-36016934 None:intergenic
GTGTAAAAGCTGTACAGATC+TGG 0.448963 2:-36018127 None:intergenic
CAAACATTGCTTGCTCCTCT+AGG 0.449369 2:-36016877 None:intergenic
TCCTGATGTGCCTGCAGGAA+TGG 0.488004 2:+36017970 MsG0280008660.01.T01:three_prime_UTR
GTCATTATGGTTGATGGTGG+TGG 0.514993 2:+36016789 None:intergenic
AAATTTCCTGATGTGCCTGC+AGG 0.517994 2:+36017965 MsG0280008660.01.T01:three_prime_UTR
TGTGACATTTCCATTCCTGC+AGG 0.523355 2:-36017980 None:intergenic
TTGCTCCTCTAGGGTAAAGC+AGG 0.536954 2:-36016867 None:intergenic
AAACATTGCTTGCTCCTCTA+GGG 0.540478 2:-36016876 None:intergenic
GAAATGTCACAACTTTATTG+TGG 0.542581 2:+36017992 MsG0280008660.01.T01:three_prime_UTR
TTAACTAAACACACTAAGCA+TGG 0.544166 2:-36017002 None:intergenic
AAGATTTATAGTGTTACAGC+AGG 0.559794 2:-36018068 None:intergenic
AACACTATAAATCTTGTGCC+AGG 0.563885 2:+36018076 MsG0280008660.01.T01:three_prime_UTR
ATGTCACAACTTTATTGTGG+AGG 0.583864 2:+36017995 MsG0280008660.01.T01:three_prime_UTR
ACATTATGTGTACTGGGTGT+AGG 0.596546 2:+36016839 MsG0280008660.01.T01:CDS
TTGTAAATATATATCATACC+TGG 0.615973 2:-36018094 None:intergenic
AACATCCTGCTTTACCCTAG+AGG 0.636037 2:+36016862 MsG0280008660.01.T01:CDS
ACAGTAAAAGGAAAGATACC+AGG 0.636834 2:-36016946 None:intergenic
TACACCCAGTACACATAATG+TGG 0.639256 2:-36016837 None:intergenic
GGAGCCACATTATGTGTACT+GGG 0.663903 2:+36016833 MsG0280008660.01.T01:CDS
GAGGAAGAACAGGAATAACA+GGG 0.675525 2:-36016924 None:intergenic
ACATTGCTAATGCACGCACG+TGG 0.683036 2:+36018025 MsG0280008660.01.T01:three_prime_UTR
GTTACAGCAGGAACATCTCA+CGG 0.690666 2:-36018056 None:intergenic
GTAAAAGGAAAGATACCAGG+AGG 0.700150 2:-36016943 None:intergenic
AGGAAGAACAGGAATAACAG+GGG 0.773701 2:-36016923 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAACAACAATTAAATT+TGG + Chr2:36017233-36017252 MsG0280008660.01.T01:intron 10.0%
!! GTTAAAAAAAAAAAAATCAA+AGG - Chr2:36017071-36017090 None:intergenic 10.0%
!! TAAAAACAACAATTAAATTT+GGG + Chr2:36017234-36017253 MsG0280008660.01.T01:intron 10.0%
!! TTATTTATACATTAAAAGAA+TGG + Chr2:36017203-36017222 MsG0280008660.01.T01:intron 10.0%
!! AAAAAAAAAAAATCAAAGGA+AGG - Chr2:36017067-36017086 None:intergenic 15.0%
!! GATTTAAACTATAAACTAAA+TGG + Chr2:36017925-36017944 MsG0280008660.01.T01:intron 15.0%
!!! ATTTAATTGTTGTTTTTATG+AGG - Chr2:36017232-36017251 None:intergenic 15.0%
!!! TTTAATTGTTGTTTTTATGA+GGG - Chr2:36017231-36017250 None:intergenic 15.0%
!! AATAATATTTCCCACTAAAT+TGG - Chr2:36017188-36017207 None:intergenic 20.0%
!! ATAATATTTCCCACTAAATT+GGG - Chr2:36017187-36017206 None:intergenic 20.0%
!! ATTTAGCCAATTATTAACAA+GGG + Chr2:36017834-36017853 MsG0280008660.01.T01:intron 20.0%
!! TATTTAGCCAATTATTAACA+AGG + Chr2:36017833-36017852 MsG0280008660.01.T01:intron 20.0%
!! TTGAAAACAAAAACAGTAAA+AGG - Chr2:36016961-36016980 None:intergenic 20.0%
!! TTGTAAATATATATCATACC+TGG - Chr2:36018097-36018116 None:intergenic 20.0%
!!! TGTTGAGTTAATTTTTCTAT+TGG - Chr2:36017368-36017387 None:intergenic 20.0%
!!! TTAATTGTTGTTTTTATGAG+GGG - Chr2:36017230-36017249 None:intergenic 20.0%
! AAATCATATACCCAATTTAG+TGG + Chr2:36017174-36017193 MsG0280008660.01.T01:intron 25.0%
! AATCATATACCCAATTTAGT+GGG + Chr2:36017175-36017194 MsG0280008660.01.T01:intron 25.0%
! CTAAATTGGGTATATGATTT+CGG - Chr2:36017174-36017193 None:intergenic 25.0%
! GACTAATAACGATCAATATA+GGG - Chr2:36017800-36017819 None:intergenic 25.0%
! TTAAATTGAAAACTCCTTCT+TGG + Chr2:36017637-36017656 MsG0280008660.01.T01:intron 25.0%
!! GAACAAGTGAATTTTTAATC+TGG - Chr2:36017902-36017921 None:intergenic 25.0%
!!! TAATTGTTGTTTTTATGAGG+GGG - Chr2:36017229-36017248 None:intergenic 25.0%
AAAACTTACAACTTAATCGC+AGG - Chr2:36017310-36017329 None:intergenic 30.0%
AAACTAAATGGTTACATCCA+GGG + Chr2:36017937-36017956 MsG0280008660.01.T01:intron 30.0%
AAACTCCTTCTTGGAATAAT+CGG + Chr2:36017646-36017665 MsG0280008660.01.T01:intron 30.0%
AAACTTACAACTTAATCGCA+GGG - Chr2:36017309-36017328 None:intergenic 30.0%
AAGATTTATAGTGTTACAGC+AGG - Chr2:36018071-36018090 None:intergenic 30.0%
ATGCATCCCTTGTTAATAAT+TGG - Chr2:36017843-36017862 None:intergenic 30.0%
GAAATGTCACAACTTTATTG+TGG + Chr2:36017992-36018011 MsG0280008660.01.T01:three_prime_UTR 30.0%
GGACTAATAACGATCAATAT+AGG - Chr2:36017801-36017820 None:intergenic 30.0%
GGCTAAATAGACTATAGATA+TGG - Chr2:36017822-36017841 None:intergenic 30.0%
TAAACTAAATGGTTACATCC+AGG + Chr2:36017936-36017955 MsG0280008660.01.T01:intron 30.0%
TATGACAAAAGTTACTCCTT+TGG - Chr2:36017528-36017547 None:intergenic 30.0%
TCAACAAAGAGTTGTTAAGT+GGG - Chr2:36017556-36017575 None:intergenic 30.0%
TTAACTAAACACACTAAGCA+TGG - Chr2:36017005-36017024 None:intergenic 30.0%
! GTTGTAAGTTTTTGCAGTAA+TGG + Chr2:36017318-36017337 MsG0280008660.01.T01:intron 30.0%
!! TGTTTAATTTTGCAGCTATG+CGG + Chr2:36016812-36016831 MsG0280008660.01.T01:CDS 30.0%
!!! AATGCTACACTAGTTTTCAT+CGG - Chr2:36017461-36017480 None:intergenic 30.0%
AACACTATAAATCTTGTGCC+AGG + Chr2:36018076-36018095 MsG0280008660.01.T01:three_prime_UTR 35.0%
ACAGTAAAAGGAAAGATACC+AGG - Chr2:36016949-36016968 None:intergenic 35.0%
ACCTTCAATACATAAACGCA+TGG - Chr2:36017692-36017711 None:intergenic 35.0%
ATGTCACAACTTTATTGTGG+AGG + Chr2:36017995-36018014 MsG0280008660.01.T01:three_prime_UTR 35.0%
ATTGCATACTTAGACTAAGC+AGG + Chr2:36017029-36017048 MsG0280008660.01.T01:intron 35.0%
CAACAAAGAGTTGTTAAGTG+GGG - Chr2:36017555-36017574 None:intergenic 35.0%
CTTCTTGTCTGATCCAAATT+AGG + Chr2:36017748-36017767 MsG0280008660.01.T01:intron 35.0%
GCATATCATCAACTTACCAA+AGG + Chr2:36017509-36017528 MsG0280008660.01.T01:intron 35.0%
GTCAACAAAGAGTTGTTAAG+TGG - Chr2:36017557-36017576 None:intergenic 35.0%
TCCATGCGTTTATGTATTGA+AGG + Chr2:36017688-36017707 MsG0280008660.01.T01:intron 35.0%
TGATCCAAATTAGGATCTAG+AGG + Chr2:36017757-36017776 MsG0280008660.01.T01:intron 35.0%
TGCGTTTATGTATTGAAGGT+AGG + Chr2:36017692-36017711 MsG0280008660.01.T01:intron 35.0%
TTGCATACTTAGACTAAGCA+GGG + Chr2:36017030-36017049 MsG0280008660.01.T01:intron 35.0%
! ATCAGGAAATTTTAGATCCC+TGG - Chr2:36017957-36017976 None:intergenic 35.0%
! CACCAAAAAGCATATGAAGA+TGG - Chr2:36017487-36017506 None:intergenic 35.0%
!!! ATGCTACACTAGTTTTCATC+GGG - Chr2:36017460-36017479 None:intergenic 35.0%
AAACATTGCTTGCTCCTCTA+GGG - Chr2:36016879-36016898 None:intergenic 40.0%
AATCAAAGGAAGGATCAACG+CGG - Chr2:36017057-36017076 None:intergenic 40.0%
ACATTATGTGTACTGGGTGT+AGG + Chr2:36016839-36016858 MsG0280008660.01.T01:CDS 40.0%
AGGAAGAACAGGAATAACAG+GGG - Chr2:36016926-36016945 None:intergenic 40.0%
CCAAATTAGGATCTAGAGGT+TGG + Chr2:36017761-36017780 MsG0280008660.01.T01:intron 40.0%
CCAACCTCTAGATCCTAATT+TGG - Chr2:36017764-36017783 None:intergenic 40.0%
GAGGAAGAACAGGAATAACA+GGG - Chr2:36016927-36016946 None:intergenic 40.0%
GTAAAAGGAAAGATACCAGG+AGG - Chr2:36016946-36016965 None:intergenic 40.0%
GTGTAAAAGCTGTACAGATC+TGG - Chr2:36018130-36018149 None:intergenic 40.0%
TACACCCAGTACACATAATG+TGG - Chr2:36016840-36016859 None:intergenic 40.0%
TGGATCCGATTATTCCAAGA+AGG - Chr2:36017654-36017673 None:intergenic 40.0%
! CGCCATCTTCATATGCTTTT+TGG + Chr2:36017482-36017501 MsG0280008660.01.T01:intron 40.0%
!! GATCTAGAGGTTGGTAAAGA+TGG + Chr2:36017770-36017789 MsG0280008660.01.T01:intron 40.0%
!!! ACTAGTTTTCATCGGGAATG+AGG - Chr2:36017453-36017472 None:intergenic 40.0%
AAAACGTGAGTTTCGAACCC+AGG + Chr2:36017108-36017127 MsG0280008660.01.T01:intron 45.0%
AAATTTCCTGATGTGCCTGC+AGG + Chr2:36017965-36017984 MsG0280008660.01.T01:three_prime_UTR 45.0%
AACATCCTGCTTTACCCTAG+AGG + Chr2:36016862-36016881 MsG0280008660.01.T01:CDS 45.0%
AAGATACCAGGAGGAAGAAC+AGG - Chr2:36016937-36016956 None:intergenic 45.0%
ATCAAAGGAAGGATCAACGC+GGG - Chr2:36017056-36017075 None:intergenic 45.0%
CAAACATTGCTTGCTCCTCT+AGG - Chr2:36016880-36016899 None:intergenic 45.0%
GGAAAAACCAACAGCCAAAG+TGG - Chr2:36017347-36017366 None:intergenic 45.0%
GGAGCCACATTATGTGTACT+GGG + Chr2:36016833-36016852 MsG0280008660.01.T01:CDS 45.0%
GGAGGAAGAACAGGAATAAC+AGG - Chr2:36016928-36016947 None:intergenic 45.0%
GTTACAGCAGGAACATCTCA+CGG - Chr2:36018059-36018078 None:intergenic 45.0%
GTTATTCCTGTTCTTCCTCC+TGG + Chr2:36016928-36016947 MsG0280008660.01.T01:CDS 45.0%
TGTGACATTTCCATTCCTGC+AGG - Chr2:36017983-36018002 None:intergenic 45.0%
TTACAACTTAATCGCAGGGC+CGG - Chr2:36017305-36017324 None:intergenic 45.0%
! TTTGGGCCCTCCAAATTTTG+TGG + Chr2:36017251-36017270 MsG0280008660.01.T01:intron 45.0%
!! ATATGATTTCGGTGCTGCAG+TGG - Chr2:36017163-36017182 None:intergenic 45.0%
!! TGCAGTAATGGATGCCACTT+TGG + Chr2:36017330-36017349 MsG0280008660.01.T01:intron 45.0%
AATTATGTCCCCCGTGACCT+GGG - Chr2:36017128-36017147 None:intergenic 50.0%
ACATTGCTAATGCACGCACG+TGG + Chr2:36018025-36018044 MsG0280008660.01.T01:three_prime_UTR 50.0%
CATGGACACACATACGTGTG+TGG - Chr2:36017674-36017693 None:intergenic 50.0%
CCAGGGCCACAAAATTTGGA+GGG - Chr2:36017260-36017279 None:intergenic 50.0%
CGGAGCCACATTATGTGTAC+TGG + Chr2:36016832-36016851 MsG0280008660.01.T01:CDS 50.0%
GGCCTGATTGCACTTGCTTT+GGG + Chr2:36017279-36017298 MsG0280008660.01.T01:intron 50.0%
TAATTATGTCCCCCGTGACC+TGG - Chr2:36017129-36017148 None:intergenic 50.0%
TGAGTTTCGAACCCAGGTCA+CGG + Chr2:36017114-36017133 MsG0280008660.01.T01:intron 50.0%
! CCTCCAAATTTTGTGGCCCT+GGG + Chr2:36017258-36017277 MsG0280008660.01.T01:intron 50.0%
! TGATTGCACTTGCTTTGGGC+CGG + Chr2:36017283-36017302 MsG0280008660.01.T01:intron 50.0%
!! ATGGATGCCACTTTGGCTGT+TGG + Chr2:36017337-36017356 MsG0280008660.01.T01:intron 50.0%
!! TTGCTCCTCTAGGGTAAAGC+AGG - Chr2:36016870-36016889 None:intergenic 50.0%
AAGCAAGTGCAATCAGGCCC+AGG - Chr2:36017278-36017297 None:intergenic 55.0%
AGCAAGTGCAATCAGGCCCA+GGG - Chr2:36017277-36017296 None:intergenic 55.0%
AGGCCCAGGGCCACAAAATT+TGG - Chr2:36017264-36017283 None:intergenic 55.0%
AGTTTCGAACCCAGGTCACG+GGG + Chr2:36017116-36017135 MsG0280008660.01.T01:intron 55.0%
CCCAGGGCCACAAAATTTGG+AGG - Chr2:36017261-36017280 None:intergenic 55.0%
GAGTTTCGAACCCAGGTCAC+GGG + Chr2:36017115-36017134 MsG0280008660.01.T01:intron 55.0%
GGCCCAAAGCAAGTGCAATC+AGG - Chr2:36017284-36017303 None:intergenic 55.0%
GGGCCTGATTGCACTTGCTT+TGG + Chr2:36017278-36017297 MsG0280008660.01.T01:intron 55.0%
TCCATTCCTGCAGGCACATC+AGG - Chr2:36017974-36017993 None:intergenic 55.0%
TCCTGATGTGCCTGCAGGAA+TGG + Chr2:36017970-36017989 MsG0280008660.01.T01:three_prime_UTR 55.0%
! CCCTCCAAATTTTGTGGCCC+TGG + Chr2:36017257-36017276 MsG0280008660.01.T01:intron 55.0%
GTTTCGAACCCAGGTCACGG+GGG + Chr2:36017117-36017136 MsG0280008660.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 36016802 36018159 36016802 ID=MsG0280008660.01;Name=MsG0280008660.01
Chr2 mRNA 36016802 36018159 36016802 ID=MsG0280008660.01.T01;Parent=MsG0280008660.01;Name=MsG0280008660.01.T01;_AED=0.48;_eAED=0.76;_QI=0|0|0|1|0|0|2|201|69
Chr2 exon 36016802 36017010 36016802 ID=MsG0280008660.01.T01:exon:15463;Parent=MsG0280008660.01.T01
Chr2 exon 36017958 36018159 36017958 ID=MsG0280008660.01.T01:exon:15464;Parent=MsG0280008660.01.T01
Chr2 CDS 36016802 36017010 36016802 ID=MsG0280008660.01.T01:cds;Parent=MsG0280008660.01.T01
Chr2 CDS 36017958 36017958 36017958 ID=MsG0280008660.01.T01:cds;Parent=MsG0280008660.01.T01
Chr2 three_prime_UTR 36017959 36018159 36017959 ID=MsG0280008660.01.T01:three_prime_utr;Parent=MsG0280008660.01.T01
Gene Sequence

>MsG0280008660.01.T01

ATGGTGGTGGTGTTTAATTTTGCAGCTATGCGGAGCCACATTATGTGTACTGGGTGTAGGAACATCCTGCTTTACCCTAGAGGAGCAAGCAATGTTTGCTGTGCAGTGTGCAGCACAATTACCCCTGTTATTCCTGTTCTTCCTCCTGGTATCTTTCCTTTTACTGTTTTTGTTTTCAATATTTCAAGAATTTTCTTGCTCCATGCTTAG

Protein sequence

>MsG0280008660.01.T01

MVVVFNFAAMRSHIMCTGCRNILLYPRGASNVCCAVCSTITPVIPVLPPGIFPFTVFVFNISRIFLLHA*