AlfalfaGEDB | MsG0580025363.01

M. sativa cultivar ZhongmuNo.1 / MsG0580025363.01

KEGG: K08829
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025363.01.T01	XP_003612616.1	72.477	109	20	1	4	102	341	449	6.86E-40	147

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025363.01.T01	G7K6V4	72.477	109	20	1	4	102	341	449	3.28e-40	147

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000355.01	MsG0580025363.01	-0.811529	6.690828e-51	4.151702e-48
MsG0180002686.01	MsG0580025363.01	0.803539	3.322590e-49	1.674084e-46
MsG0180003793.01	MsG0580025363.01	-0.806658	7.391041e-50	4.034038e-47
MsG0180004293.01	MsG0580025363.01	0.801463	8.898439e-49	4.252193e-46
MsG0180004398.01	MsG0580025363.01	-0.807634	4.592194e-50	2.570828e-47
MsG0180004605.01	MsG0580025363.01	0.818837	1.590952e-52	1.204158e-49
MsG0180004854.01	MsG0580025363.01	-0.803132	4.033973e-49	2.011204e-46
MsG0180004861.01	MsG0580025363.01	-0.804409	2.190589e-49	1.128588e-46
MsG0280011304.01	MsG0580025363.01	-0.801947	7.080654e-49	3.425237e-46
MsG0380012122.01	MsG0580025363.01	-0.809080	2.258361e-50	1.313322e-47
MsG0480021037.01	MsG0580025363.01	-0.825269	5.138959e-54	4.661721e-51
MsG0480021706.01	MsG0580025363.01	-0.827152	1.831705e-54	1.753610e-51
MsG0480021876.01	MsG0580025363.01	0.822221	2.659650e-53	2.212343e-50
MsG0480022781.01	MsG0580025363.01	0.820820	5.605486e-53	4.482619e-50
MsG0480023652.01	MsG0580025363.01	-0.800180	1.626773e-48	7.524996e-46
MsG0580024619.01	MsG0580025363.01	-0.803313	3.699936e-49	1.853277e-46
MsG0580025362.01	MsG0580025363.01	0.893348	6.835147e-75	6.487569e-71
MsG0580025363.01	MsG0580026485.01	-0.816157	6.392274e-52	4.494827e-49
MsG0580025363.01	MsG0680031000.01	-0.801631	8.221867e-49	3.945505e-46
MsG0580025363.01	MsG0680031628.01	-0.812605	3.896763e-51	2.488555e-48
MsG0580025363.01	MsG0680035465.01	0.801326	9.493632e-49	4.520957e-46
MsG0580025363.01	MsG0680035765.01	-0.815183	1.053207e-51	7.210345e-49
MsG0580025363.01	MsG0780036186.01	-0.806718	7.181010e-50	3.925637e-47
MsG0580025363.01	MsG0780036544.01	0.861369	1.076825e-63	3.040224e-60
MsG0580025363.01	MsG0780037093.01	-0.825261	5.163352e-54	4.682761e-51
MsG0580025363.01	MsG0780038400.01	-0.806107	9.660415e-50	5.199306e-47
MsG0580025363.01	MsG0780041223.01	-0.828437	8.996185e-55	8.941738e-52
MsG0580025363.01	MsG0780041268.01	-0.834617	2.702497e-56	3.224272e-53
MsG0580025363.01	MsG0880045480.01	0.813681	2.263184e-51	1.487732e-48
MsG0580025363.01	MsG0880045684.01	-0.814286	1.664944e-51	1.112553e-48
MsG0580025363.01	MsG0880045705.01	0.815613	8.451504e-52	5.855460e-49
MsG0580025363.01	MsG0880047703.01	-0.801245	9.860947e-49	4.686526e-46
MsG0380015598.01	MsG0580025363.01	0.818262	2.148983e-52	1.600689e-49
MsG0380016005.01	MsG0580025363.01	-0.810837	9.450980e-51	5.758135e-48
MsG0380018033.01	MsG0580025363.01	-0.800307	1.532463e-48	7.112298e-46
MsG0280007847.01	MsG0580025363.01	-0.826113	3.241515e-54	3.011924e-51
MsG0280010082.01	MsG0580025363.01	-0.809264	2.061918e-50	1.205022e-47
MsG0280010532.01	MsG0580025363.01	0.808154	3.559533e-50	2.020195e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025363.01.T01	MTR_5g026960	72.477	109	20	1	4	102	341	449	8.31e-44	147
MsG0580025363.01.T01	MTR_8g071230	54.630	108	39	1	5	102	344	451	1.32e-28	107

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025363.01.T01	AT4G19110	43.443	122	42	6	4	102	344	461	9.33e-14	65.9
MsG0580025363.01.T01	AT4G19110	45.600	125	38	8	4	102	344	464	1.19e-13	65.5
MsG0580025363.01.T01	AT4G19110	46.281	121	39	8	4	102	288	404	3.50e-13	64.3

Find 29 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCAATTGACTCCAGCTTTCA+TGG	0.296266	5:-17508342	None:intergenic
TTGGCAGCTTCTGTGATTAA+AGG	0.299609	5:+17508224	MsG0580025363.01.T01:CDS
TCGATTACCAATAGACATAT+TGG	0.387098	5:-17508288	None:intergenic
CAGCTTTCATGGGAGGAGGC+CGG	0.413750	5:-17508331	None:intergenic
CCATGAAAGCTGGAGTCAAT+TGG	0.422378	5:+17508342	MsG0580025363.01.T01:CDS
TACAGGTGTGCAACGGAAGC+TGG	0.426860	5:+17507424	MsG0580025363.01.T01:CDS
CCGGTCGGAATCTGTGGTGC+AGG	0.426933	5:-17508395	None:intergenic
TGTGCAACGGAAGCTGGATA+TGG	0.431649	5:+17507430	MsG0580025363.01.T01:CDS
AGCTTTCATGGGAGGAGGCC+GGG	0.440952	5:-17508330	None:intergenic
CACCCAATTATGAATGATAC+AGG	0.441248	5:+17507407	None:intergenic
ACATACAGTAGAAAAGTTGC+TGG	0.446677	5:+17508422	MsG0580025363.01.T01:CDS
GAAGCTGGATATGGTAAATC+AGG	0.452827	5:+17507439	MsG0580025363.01.T01:CDS
GCAGGCCTGAGCATGAAGTT+AGG	0.461998	5:-17508377	None:intergenic
CGGCCTCCTCCCATGAAAGC+TGG	0.466570	5:+17508332	MsG0580025363.01.T01:CDS
AGGAGGCCGGGTTTGCCACA+TGG	0.479508	5:-17508318	None:intergenic
GACAATCCATGTGGCAAACC+CGG	0.480047	5:+17508312	MsG0580025363.01.T01:CDS
AGGTTGGCCAATATGTCTAT+TGG	0.510546	5:+17508281	MsG0580025363.01.T01:CDS
AATCTCCTAACTTCATGCTC+AGG	0.513765	5:+17508372	MsG0580025363.01.T01:CDS
TTATTTACGGAAATTTGGGT+TGG	0.517240	5:+17508198	MsG0580025363.01.T01:CDS
CAATTGACTCCAGCTTTCAT+GGG	0.520853	5:-17508341	None:intergenic
TTCAGAAACAGCTGAGAGGT+TGG	0.522598	5:+17508265	MsG0580025363.01.T01:CDS
CTACTGTATGTTCTTCCGGT+CGG	0.527606	5:-17508410	None:intergenic
ACTCCAGCTTTCATGGGAGG+AGG	0.550899	5:-17508335	None:intergenic
GTTCTTCCGGTCGGAATCTG+TGG	0.558866	5:-17508401	None:intergenic
CCTGCACCACAGATTCCGAC+CGG	0.597874	5:+17508395	MsG0580025363.01.T01:CDS
TTGACTCCAGCTTTCATGGG+AGG	0.604223	5:-17508338	None:intergenic
TGAATGATACAGGTGTGCAA+CGG	0.657899	5:+17507417	MsG0580025363.01.T01:CDS
AGATTTCAGAAACAGCTGAG+AGG	0.692644	5:+17508261	MsG0580025363.01.T01:CDS
GTAATCGAAGACAATCCATG+TGG	0.773300	5:+17508303	MsG0580025363.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACAATAATTAAACAAAAAAA+CGG	-	Chr5:17507767-17507786	None:intergenic	10.0%
!!!	TTTTGTTTTTAATTTGAGAA+TGG	+	Chr5:17507544-17507563	MsG0580025363.01.T01:intron	15.0%
!!!	TTTTTGTTTAATTATTGTTC+CGG	+	Chr5:17507769-17507788	MsG0580025363.01.T01:intron	15.0%
!!	AAGAATCAGAGATAAATTAT+TGG	-	Chr5:17507648-17507667	None:intergenic	20.0%
!!!	AGAAGTGTTGTTTTTATTTA+CGG	+	Chr5:17508185-17508204	MsG0580025363.01.T01:CDS	20.0%
!!!	ATTGATTTTTTTTGAGATCA+TGG	-	Chr5:17507525-17507544	None:intergenic	20.0%
!!!	GTTTTTATTTACGGAAATTT+GGG	+	Chr5:17508194-17508213	MsG0580025363.01.T01:CDS	20.0%
!!!	TGTTTTTATTTACGGAAATT+TGG	+	Chr5:17508193-17508212	MsG0580025363.01.T01:CDS	20.0%
!!!	TTGATTTTTTTTGAGATCAT+GGG	-	Chr5:17507524-17507543	None:intergenic	20.0%
!!!	TTTATCTCTGATTCTTTTAA+AGG	+	Chr5:17507652-17507671	MsG0580025363.01.T01:intron	20.0%
!!!	TTTTTTTTTTTCCCTTTCTT+GGG	+	Chr5:17507571-17507590	MsG0580025363.01.T01:intron	20.0%
!!!	TTTTTTTTTTTTCCCTTTCT+TGG	+	Chr5:17507570-17507589	MsG0580025363.01.T01:intron	20.0%
!	AATGAACCTTCTTGAAAAAT+GGG	-	Chr5:17507988-17508007	None:intergenic	25.0%
!	GTAAATCTTAACATGATGAT+GGG	+	Chr5:17508105-17508124	MsG0580025363.01.T01:intron	25.0%
!	TGTAAATCTTAACATGATGA+TGG	+	Chr5:17508104-17508123	MsG0580025363.01.T01:intron	25.0%
!!!	ATAGTTACTCTGAAATTTAC+TGG	+	Chr5:17508155-17508174	MsG0580025363.01.T01:intron	25.0%
!!!	TATATTCCCATTTTTCAAGA+AGG	+	Chr5:17507979-17507998	MsG0580025363.01.T01:intron	25.0%
!!!	TGATTTTTTTTGAGATCATG+GGG	-	Chr5:17507523-17507542	None:intergenic	25.0%
	AAAAGAAGGGAAAAAACAGA+AGG	-	Chr5:17507876-17507895	None:intergenic	30.0%
	GAATGAACCTTCTTGAAAAA+TGG	-	Chr5:17507989-17508008	None:intergenic	30.0%
	TAAAATTTCTCAACGACATG+AGG	-	Chr5:17507949-17507968	None:intergenic	30.0%
	TATATGTATGTGGTTAGGAA+TGG	-	Chr5:17508024-17508043	None:intergenic	30.0%
	TCAAACAGCCCTAAAAAAAT+AGG	-	Chr5:17507914-17507933	None:intergenic	30.0%
	TCGATTACCAATAGACATAT+TGG	-	Chr5:17508291-17508310	None:intergenic	30.0%
	TTACTCGATGTATATGTATG+TGG	-	Chr5:17508034-17508053	None:intergenic	30.0%
	TTATTTACGGAAATTTGGGT+TGG	+	Chr5:17508198-17508217	MsG0580025363.01.T01:CDS	30.0%
!	TTTTCTACTGTATGTTCTTC+CGG	-	Chr5:17508417-17508436	None:intergenic	30.0%
!	TTTTGGGCAAAATGATTGAT+CGG	+	Chr5:17507798-17507817	MsG0580025363.01.T01:intron	30.0%
!!	TCTCTGATTCTTTTAAAGGA+TGG	+	Chr5:17507656-17507675	MsG0580025363.01.T01:intron	30.0%
!!!	TCTGTTTTTTCCCTTCTTTT+GGG	+	Chr5:17507876-17507895	MsG0580025363.01.T01:intron	30.0%
!!!	TTCCCTCTCCTATTTTTTTA+GGG	+	Chr5:17507903-17507922	MsG0580025363.01.T01:intron	30.0%
!!!	TTCTGTTTTTTCCCTTCTTT+TGG	+	Chr5:17507875-17507894	MsG0580025363.01.T01:intron	30.0%
	AACTATTCATACTTCGCTCA+AGG	-	Chr5:17508141-17508160	None:intergenic	35.0%
	AAGATTTACACCAGATGAGA+GGG	-	Chr5:17508094-17508113	None:intergenic	35.0%
	ACATACAGTAGAAAAGTTGC+TGG	+	Chr5:17508422-17508441	MsG0580025363.01.T01:CDS	35.0%
	AGCCCTAAAAAAATAGGAGA+GGG	-	Chr5:17507908-17507927	None:intergenic	35.0%
	CGATGTATATGTATGTGGTT+AGG	-	Chr5:17508029-17508048	None:intergenic	35.0%
	TAAGATTTACACCAGATGAG+AGG	-	Chr5:17508095-17508114	None:intergenic	35.0%
	TTTGGGCAAAATGATTGATC+GGG	+	Chr5:17507799-17507818	MsG0580025363.01.T01:intron	35.0%
!	ATTGTTCCGGATAGTCTTTT+GGG	+	Chr5:17507782-17507801	MsG0580025363.01.T01:intron	35.0%
!	ATTTTGCCCAAAAGACTATC+CGG	-	Chr5:17507791-17507810	None:intergenic	35.0%
!	TATTGTTCCGGATAGTCTTT+TGG	+	Chr5:17507781-17507800	MsG0580025363.01.T01:intron	35.0%
!!	AACGGACTGTAAAACTTACT+TGG	-	Chr5:17507749-17507768	None:intergenic	35.0%
!!!	CTGTTTTTTCCCTTCTTTTG+GGG	+	Chr5:17507877-17507896	MsG0580025363.01.T01:intron	35.0%
!!!	GTTCCCTCTCCTATTTTTTT+AGG	+	Chr5:17507902-17507921	MsG0580025363.01.T01:intron	35.0%
	AAATGATTGATCGGGATTGG+AGG	+	Chr5:17507807-17507826	MsG0580025363.01.T01:intron	40.0%
	AATCTCCTAACTTCATGCTC+AGG	+	Chr5:17508372-17508391	MsG0580025363.01.T01:CDS	40.0%
	AATGATTGATCGGGATTGGA+GGG	+	Chr5:17507808-17507827	MsG0580025363.01.T01:intron	40.0%
	AGATTTCAGAAACAGCTGAG+AGG	+	Chr5:17508261-17508280	MsG0580025363.01.T01:CDS	40.0%
	ATACGTCACTATCCCAAGAA+AGG	-	Chr5:17507586-17507605	None:intergenic	40.0%
	CAGCCCTAAAAAAATAGGAG+AGG	-	Chr5:17507909-17507928	None:intergenic	40.0%
	GAAGCTGGATATGGTAAATC+AGG	+	Chr5:17507439-17507458	MsG0580025363.01.T01:CDS	40.0%
	GCAAAATGATTGATCGGGAT+TGG	+	Chr5:17507804-17507823	MsG0580025363.01.T01:intron	40.0%
	GTAATCGAAGACAATCCATG+TGG	+	Chr5:17508303-17508322	MsG0580025363.01.T01:CDS	40.0%
	TACGTCACTATCCCAAGAAA+GGG	-	Chr5:17507585-17507604	None:intergenic	40.0%
	TACTTGGACTTTGCTTTCCT+GGG	-	Chr5:17507733-17507752	None:intergenic	40.0%
	TGAATGATACAGGTGTGCAA+CGG	+	Chr5:17507417-17507436	MsG0580025363.01.T01:CDS	40.0%
	TTACTTGGACTTTGCTTTCC+TGG	-	Chr5:17507734-17507753	None:intergenic	40.0%
	TTGGCAGCTTCTGTGATTAA+AGG	+	Chr5:17508224-17508243	MsG0580025363.01.T01:CDS	40.0%
!	AGGTTGGCCAATATGTCTAT+TGG	+	Chr5:17508281-17508300	MsG0580025363.01.T01:CDS	40.0%
!	CAATTGACTCCAGCTTTCAT+GGG	-	Chr5:17508344-17508363	None:intergenic	40.0%
!!!	CGGAAATTTGGGTTGGTTTT+TGG	+	Chr5:17508205-17508224	MsG0580025363.01.T01:CDS	40.0%
	AGGGAACATCCCCAAAAGAA+GGG	-	Chr5:17507889-17507908	None:intergenic	45.0%
	CAATCGAAATATCGACACCC+AGG	+	Chr5:17507713-17507732	MsG0580025363.01.T01:intron	45.0%
	CCATGAAAGCTGGAGTCAAT+TGG	+	Chr5:17508342-17508361	MsG0580025363.01.T01:CDS	45.0%
	CTACTGTATGTTCTTCCGGT+CGG	-	Chr5:17508413-17508432	None:intergenic	45.0%
	GATGTGCATTGTCATGTAGC+TGG	-	Chr5:17507852-17507871	None:intergenic	45.0%
	TATGAGTCCACAGTGTTACC+TGG	+	Chr5:17508055-17508074	MsG0580025363.01.T01:intron	45.0%
	TGGGGTAAAAGAATGTGTGC+AGG	-	Chr5:17507505-17507524	None:intergenic	45.0%
	TTCAGAAACAGCTGAGAGGT+TGG	+	Chr5:17508265-17508284	MsG0580025363.01.T01:CDS	45.0%
!	CCAATTGACTCCAGCTTTCA+TGG	-	Chr5:17508345-17508364	None:intergenic	45.0%
!!!	GAGGGTTTTGGGTGAAAGAT+GGG	+	Chr5:17507826-17507845	MsG0580025363.01.T01:intron	45.0%
	GACAATCCATGTGGCAAACC+CGG	+	Chr5:17508312-17508331	MsG0580025363.01.T01:CDS	50.0%
	GAGGGAACATCCCCAAAAGA+AGG	-	Chr5:17507890-17507909	None:intergenic	50.0%
	TGTGCAACGGAAGCTGGATA+TGG	+	Chr5:17507430-17507449	MsG0580025363.01.T01:CDS	50.0%
	TTACACCAGATGAGAGGGCT+GGG	-	Chr5:17508089-17508108	None:intergenic	50.0%
	TTGACTCCAGCTTTCATGGG+AGG	-	Chr5:17508341-17508360	None:intergenic	50.0%
	TTTACACCAGATGAGAGGGC+TGG	-	Chr5:17508090-17508109	None:intergenic	50.0%
!	GATCGGGATTGGAGGGTTTT+GGG	+	Chr5:17507815-17507834	MsG0580025363.01.T01:intron	50.0%
!	TGATCGGGATTGGAGGGTTT+TGG	+	Chr5:17507814-17507833	MsG0580025363.01.T01:intron	50.0%
!	TGATGGACCAGGTAACACTG+TGG	-	Chr5:17508065-17508084	None:intergenic	50.0%
!!!	GGAGGGTTTTGGGTGAAAGA+TGG	+	Chr5:17507825-17507844	MsG0580025363.01.T01:intron	50.0%
	ACTCCAGCTTTCATGGGAGG+AGG	-	Chr5:17508338-17508357	None:intergenic	55.0%
	GCAGGCCTGAGCATGAAGTT+AGG	-	Chr5:17508380-17508399	None:intergenic	55.0%
	GTTCTTCCGGTCGGAATCTG+TGG	-	Chr5:17508404-17508423	None:intergenic	55.0%
	TACAGGTGTGCAACGGAAGC+TGG	+	Chr5:17507424-17507443	MsG0580025363.01.T01:CDS	55.0%
	AGATGAGAGGGCTGGGCTGA+TGG	-	Chr5:17508082-17508101	None:intergenic	60.0%
	AGCTTTCATGGGAGGAGGCC+GGG	-	Chr5:17508333-17508352	None:intergenic	60.0%
	ATCAGCCCAGCCCTCTCATC+TGG	+	Chr5:17508081-17508100	MsG0580025363.01.T01:intron	60.0%
	CAGCTTTCATGGGAGGAGGC+CGG	-	Chr5:17508334-17508353	None:intergenic	60.0%
	CCTGCACCACAGATTCCGAC+CGG	+	Chr5:17508395-17508414	MsG0580025363.01.T01:CDS	60.0%
	AGGAGGCCGGGTTTGCCACA+TGG	-	Chr5:17508321-17508340	None:intergenic	65.0%
	CCGGTCGGAATCTGTGGTGC+AGG	-	Chr5:17508398-17508417	None:intergenic	65.0%
	CGGCCTCCTCCCATGAAAGC+TGG	+	Chr5:17508332-17508351	MsG0580025363.01.T01:CDS	65.0%
!	GAGGGCTGGGCTGATGGACC+AGG	-	Chr5:17508076-17508095	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	17507416	17508448	17507416	ID=MsG0580025363.01;Name=MsG0580025363.01
Chr5	mRNA	17507416	17508448	17507416	ID=MsG0580025363.01.T01;Parent=MsG0580025363.01;Name=MsG0580025363.01.T01;_AED=0.48;_eAED=0.56;_QI=0\|0\|0\|1\|0\|0\|2\|0\|102
Chr5	exon	17507416	17507460	17507416	ID=MsG0580025363.01.T01:exon:28966;Parent=MsG0580025363.01.T01
Chr5	exon	17508185	17508448	17508185	ID=MsG0580025363.01.T01:exon:28967;Parent=MsG0580025363.01.T01
Chr5	CDS	17507416	17507460	17507416	ID=MsG0580025363.01.T01:cds;Parent=MsG0580025363.01.T01
Chr5	CDS	17508185	17508448	17508185	ID=MsG0580025363.01.T01:cds;Parent=MsG0580025363.01.T01

Gene Sequence	>MsG0580025363.01.T01 ATGAATGATACAGGTGTGCAACGGAAGCTGGATATGGTAAATCAGAGAAGTGTTGTTTTTATTTACGGAAATTTGGGTTGGTTTTTGGCAGCTTCTGTGATTAAAGGAAGAACTACACATGAGATTTCAGAAACAGCTGAGAGGTTGGCCAATATGTCTATTGGTAATCGAAGACAATCCATGTGGCAAACCCGGCCTCCTCCCATGAAAGCTGGAGTCAATTGGAGTTCTGAATCTCCTAACTTCATGCTCAGGCCTGCACCACAGATTCCGACCGGAAGAACATACAGTAGAAAAGTTGCTGGATGA
Protein sequence	>MsG0580025363.01.T01 MNDTGVQRKLDMVNQRSVVFIYGNLGWFLAASVIKGRTTHEISETAERLANMSIGNRRQSMWQTRPPPMKAGVNWSSESPNFMLRPAPQIPTGRTYSRKVAG*