AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680034750.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034750.01.T01 MTR_6g073013 98.276 174 3 0 1 174 6 179 8.29e-130 361
MsG0680034750.01.T01 MTR_7g010300 38.776 196 61 5 14 155 88 278 7.23e-39 135
MsG0680034750.01.T01 MTR_1g077020 35.795 176 77 5 14 155 9 182 4.00e-34 119
MsG0680034750.01.T01 MTR_5g032600 36.979 192 69 4 14 155 78 267 2.17e-32 117
MsG0680034750.01.T01 MTR_2g076780 32.701 211 82 6 1 155 26 232 1.77e-31 114
MsG0680034750.01.T01 MTR_6g012280 35.071 211 80 7 14 171 91 297 1.42e-29 110
MsG0680034750.01.T01 MTR_8g069080 32.057 209 85 5 2 155 57 263 4.05e-29 108
MsG0680034750.01.T01 MTR_3g100650 32.292 192 78 5 14 155 80 269 2.50e-26 101
MsG0680034750.01.T01 MTR_8g467340 56.338 71 30 1 96 166 271 340 2.74e-22 92.4
MsG0680034750.01.T01 MTR_8g467340 66.667 51 16 1 14 63 64 114 5.31e-17 77.8
MsG0680034750.01.T01 MTR_4g134580 56.667 60 25 1 96 155 284 342 5.95e-20 85.5
MsG0680034750.01.T01 MTR_4g134580 62.000 50 18 1 14 63 113 161 5.16e-15 72.0
MsG0680034750.01.T01 MTR_5g020510 56.667 60 25 1 96 155 233 291 1.69e-19 83.6
MsG0680034750.01.T01 MTR_5g020510 61.538 52 15 2 14 63 99 147 9.83e-16 73.2
MsG0680034750.01.T01 MTR_8g051480 33.600 125 68 3 44 155 2 124 1.19e-17 75.1
MsG0680034750.01.T01 MTR_5g065440 53.125 64 29 1 92 155 201 263 2.23e-16 74.7
MsG0680034750.01.T01 MTR_5g065440 56.000 50 21 1 14 63 58 106 8.21e-12 62.4
MsG0680034750.01.T01 MTR_3g039810 50.794 63 30 1 1 63 7 68 2.36e-15 67.8
MsG0680034750.01.T01 MTR_8g069380 39.474 76 45 1 81 156 37 111 1.66e-14 66.6
MsG0680034750.01.T01 MTR_8g442330 33.594 128 67 5 23 134 14 139 2.16e-14 67.0
MsG0680034750.01.T01 MTR_6g012220 60.000 50 15 1 14 63 21 65 2.46e-14 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034750.01.T01 AT1G14440 41.270 189 58 6 14 153 90 274 1.40e-40 139
MsG0680034750.01.T01 AT1G14440 41.270 189 58 6 14 153 90 274 1.40e-40 139
MsG0680034750.01.T01 AT2G02540 35.981 214 75 5 14 167 87 298 3.43e-40 138
MsG0680034750.01.T01 AT2G02540 35.981 214 75 5 14 167 87 298 3.43e-40 138
MsG0680034750.01.T01 AT4G24660 42.012 169 69 3 14 155 49 215 2.53e-39 133
MsG0680034750.01.T01 AT4G24660 42.012 169 69 3 14 155 49 215 3.66e-39 133
MsG0680034750.01.T01 AT2G18350 40.678 177 68 5 14 155 82 256 5.87e-39 134
MsG0680034750.01.T01 AT5G65410 39.779 181 68 5 12 155 73 249 1.36e-38 133
MsG0680034750.01.T01 AT1G69600 39.894 188 69 6 14 158 31 217 3.83e-35 123
MsG0680034750.01.T01 AT3G28920 38.500 200 64 5 14 155 52 250 8.19e-35 124
MsG0680034750.01.T01 AT5G15210 37.634 186 68 5 14 155 56 237 1.17e-34 123
MsG0680034750.01.T01 AT5G42780 37.368 190 79 4 1 154 51 236 3.28e-34 121
MsG0680034750.01.T01 AT5G60480 39.412 170 72 5 13 153 36 203 9.43e-33 116
MsG0680034750.01.T01 AT3G50890 38.220 191 76 4 14 164 60 248 6.14e-31 112
MsG0680034750.01.T01 AT1G14687 38.554 166 72 5 13 154 6 165 2.47e-30 108
MsG0680034750.01.T01 AT1G75240 60.000 70 23 2 96 165 240 304 1.03e-23 95.1
MsG0680034750.01.T01 AT1G75240 62.000 50 18 1 14 63 76 124 1.35e-14 70.5
MsG0680034750.01.T01 AT5G39760 54.839 62 27 1 94 155 198 258 3.55e-18 80.5
MsG0680034750.01.T01 AT5G39760 48.649 74 37 1 12 84 54 127 2.34e-15 72.8
MsG0680034750.01.T01 AT1G74660 56.604 53 22 1 11 63 36 87 2.26e-15 68.6
MsG0680034750.01.T01 AT1G74660 56.604 53 22 1 11 63 36 87 4.76e-15 67.8
MsG0680034750.01.T01 AT3G28917 54.000 50 23 0 14 63 33 82 3.69e-14 65.5
MsG0680034750.01.T01 AT1G18835 54.717 53 20 1 11 63 23 71 1.65e-13 63.2

Find 51 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGGGTTTGCCGAGAAGTT+AGG 0.313045 6:+93921568 MsG0680034750.01.T01:CDS
GGAGGAGGTAGTGGAGGAGA+TGG 0.351040 6:+93921493 MsG0680034750.01.T01:CDS
GAGGAGATGGGAGGAGGAAG+AGG 0.371577 6:+93921506 MsG0680034750.01.T01:CDS
CAATTTCCACCCTAACTTCT+CGG 0.379602 6:-93921578 None:intergenic
GAATTAGCATTACCAGTAGA+AGG 0.429521 6:-93921721 None:intergenic
GAGGAGGTAGTGGAGGAGAT+GGG 0.433503 6:+93921494 MsG0680034750.01.T01:CDS
GAGGTAGTGGAGGAGATGGG+AGG 0.435653 6:+93921497 MsG0680034750.01.T01:CDS
GTAGTGGAGGAGATGGGAGG+AGG 0.441484 6:+93921500 MsG0680034750.01.T01:CDS
TGCCGCGGCCGCTGCAATGT+CGG 0.446748 6:+93921447 MsG0680034750.01.T01:CDS
TATTCCGGTGGAGGAGGTAG+TGG 0.451652 6:+93921484 MsG0680034750.01.T01:CDS
AGCCGCAGGCTGCACATTGG+AGG 0.456002 6:-93921378 None:intergenic
GCAAAATAACCGCGATCACA+AGG 0.458670 6:+93921419 MsG0680034750.01.T01:CDS
AGAAACCATGCAGCAAGCCT+TGG 0.459741 6:+93921289 MsG0680034750.01.T01:CDS
CGTCGGTGGCGTAGCTTCCA+AGG 0.465781 6:-93921306 None:intergenic
GAAATTTACGCCGGATCAGA+AGG 0.471585 6:+93921537 MsG0680034750.01.T01:CDS
ATCCGACATTGCAGCGGCCG+CGG 0.471777 6:-93921449 None:intergenic
TGGGGTTTGCCGAGAAGTTA+GGG 0.472374 6:+93921569 MsG0680034750.01.T01:CDS
AATTCTTCTGATCCTTCTAC+TGG 0.475357 6:+93921709 MsG0680034750.01.T01:CDS
GATCAGTTGATGGAATATTC+CGG 0.475527 6:+93921469 MsG0680034750.01.T01:CDS
AGCTTCCAAGGCTTGCTGCA+TGG 0.476710 6:-93921294 None:intergenic
GAAGTTCCGGTGACAGCCGC+AGG 0.479614 6:-93921392 None:intergenic
ATGGGAGGAGGAAGAGGTAC+CGG 0.496163 6:+93921512 MsG0680034750.01.T01:CDS
TGCAATGTCGGATCAGTTGA+TGG 0.497838 6:+93921459 MsG0680034750.01.T01:CDS
ATCATCTTCTCCTTCTGATC+CGG 0.499539 6:-93921547 None:intergenic
GTGCAGCCTGCGGCTGTCAC+CGG 0.504708 6:+93921386 MsG0680034750.01.T01:CDS
GGATCAGAAGGAGAAGATGA+TGG 0.514525 6:+93921549 MsG0680034750.01.T01:CDS
AACTTGATGAAGAGATTGAA+AGG 0.524348 6:+93921611 MsG0680034750.01.T01:CDS
TCCTCCACTACCTCCTCCAC+CGG 0.526819 6:-93921488 None:intergenic
GATCAGAAGGAGAAGATGAT+GGG 0.537307 6:+93921550 MsG0680034750.01.T01:CDS
GATCCGGCGTAAATTTCGTC+CGG 0.537660 6:-93921531 None:intergenic
ATGGAATATTCCGGTGGAGG+AGG 0.538823 6:+93921478 MsG0680034750.01.T01:CDS
AGCTACGCCACCGACGGCTG+CGG 0.539403 6:+93921313 MsG0680034750.01.T01:CDS
TGGAGGCTGTTTATCGCGGA+TGG 0.549396 6:-93921361 None:intergenic
GAATTCACCGCAGCCGTCGG+TGG 0.562401 6:-93921320 None:intergenic
GGTGAATTCACCGCAGCCGT+CGG 0.578066 6:-93921323 None:intergenic
GACAGCCGCAGGCTGCACAT+TGG 0.578845 6:-93921381 None:intergenic
GGCACTCTCCCTTGTGATCG+CGG 0.582002 6:-93921428 None:intergenic
TCCGGTGGAGGAGGTAGTGG+AGG 0.589804 6:+93921487 MsG0680034750.01.T01:CDS
TTGATGGAATATTCCGGTGG+AGG 0.592071 6:+93921475 MsG0680034750.01.T01:CDS
CAAAATAACCGCGATCACAA+GGG 0.605300 6:+93921420 MsG0680034750.01.T01:CDS
GGATGGCGATGAGTCGTCGA+TGG 0.613786 6:-93921344 None:intergenic
CACACACTACTGAGTAAGAG+AGG 0.616345 6:-93921743 None:intergenic
AGCCTCCAATGTGCAGCCTG+CGG 0.622915 6:+93921376 MsG0680034750.01.T01:CDS
GATCACAAGGGAGAGTGCCG+CGG 0.625206 6:+93921432 MsG0680034750.01.T01:CDS
ACATTGGAGGCTGTTTATCG+CGG 0.631854 6:-93921365 None:intergenic
CTTGGAAGCTACGCCACCGA+CGG 0.637476 6:+93921307 MsG0680034750.01.T01:CDS
ATCAGAAGGAGAAGATGATG+GGG 0.650841 6:+93921551 MsG0680034750.01.T01:CDS
CAGTTGATGGAATATTCCGG+TGG 0.694003 6:+93921472 MsG0680034750.01.T01:CDS
CAACTGATCCGACATTGCAG+CGG 0.694689 6:-93921455 None:intergenic
GGTTTGCCGAGAAGTTAGGG+TGG 0.718936 6:+93921572 MsG0680034750.01.T01:CDS
GTACCGGACGAAATTTACGC+CGG 0.738784 6:+93921528 MsG0680034750.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AACTTGATGAAGAGATTGAA+AGG + Chr6:93921611-93921630 MsG0680034750.01.T01:CDS 30.0%
! TGTGAGTAGACAAGTTTTTA+AGG + Chr6:93921651-93921670 MsG0680034750.01.T01:CDS 30.0%
AATTCTTCTGATCCTTCTAC+TGG + Chr6:93921709-93921728 MsG0680034750.01.T01:CDS 35.0%
GAATTAGCATTACCAGTAGA+AGG - Chr6:93921724-93921743 None:intergenic 35.0%
!! GAAAGGTTTTGTGAAAGTGT+TGG + Chr6:93921628-93921647 MsG0680034750.01.T01:CDS 35.0%
!! GATCAGTTGATGGAATATTC+CGG + Chr6:93921469-93921488 MsG0680034750.01.T01:CDS 35.0%
!!! GTAGACAAGTTTTTAAGGTG+TGG + Chr6:93921656-93921675 MsG0680034750.01.T01:CDS 35.0%
ATCAGAAGGAGAAGATGATG+GGG + Chr6:93921551-93921570 MsG0680034750.01.T01:CDS 40.0%
ATCATCTTCTCCTTCTGATC+CGG - Chr6:93921550-93921569 None:intergenic 40.0%
CAAAATAACCGCGATCACAA+GGG + Chr6:93921420-93921439 MsG0680034750.01.T01:CDS 40.0%
CAATTTCCACCCTAACTTCT+CGG - Chr6:93921581-93921600 None:intergenic 40.0%
GATCAGAAGGAGAAGATGAT+GGG + Chr6:93921550-93921569 MsG0680034750.01.T01:CDS 40.0%
!! TTATTTTGCGGTGGAAGTTC+CGG - Chr6:93921408-93921427 None:intergenic 40.0%
!! TTGTGATCGCGGTTATTTTG+CGG - Chr6:93921420-93921439 None:intergenic 40.0%
ACATTGGAGGCTGTTTATCG+CGG - Chr6:93921368-93921387 None:intergenic 45.0%
GAAATTTACGCCGGATCAGA+AGG + Chr6:93921537-93921556 MsG0680034750.01.T01:CDS 45.0%
GCAAAATAACCGCGATCACA+AGG + Chr6:93921419-93921438 MsG0680034750.01.T01:CDS 45.0%
GGATCAGAAGGAGAAGATGA+TGG + Chr6:93921549-93921568 MsG0680034750.01.T01:CDS 45.0%
TGCAATGTCGGATCAGTTGA+TGG + Chr6:93921459-93921478 MsG0680034750.01.T01:CDS 45.0%
TTGATGGAATATTCCGGTGG+AGG + Chr6:93921475-93921494 MsG0680034750.01.T01:CDS 45.0%
!! CAGTTGATGGAATATTCCGG+TGG + Chr6:93921472-93921491 MsG0680034750.01.T01:CDS 45.0%
AGAAACCATGCAGCAAGCCT+TGG + Chr6:93921289-93921308 MsG0680034750.01.T01:CDS 50.0%
ATGGAATATTCCGGTGGAGG+AGG + Chr6:93921478-93921497 MsG0680034750.01.T01:CDS 50.0%
ATGGGGTTTGCCGAGAAGTT+AGG + Chr6:93921568-93921587 MsG0680034750.01.T01:CDS 50.0%
CAACTGATCCGACATTGCAG+CGG - Chr6:93921458-93921477 None:intergenic 50.0%
GATCCGGCGTAAATTTCGTC+CGG - Chr6:93921534-93921553 None:intergenic 50.0%
GTACCGGACGAAATTTACGC+CGG + Chr6:93921528-93921547 MsG0680034750.01.T01:CDS 50.0%
TGGGGTTTGCCGAGAAGTTA+GGG + Chr6:93921569-93921588 MsG0680034750.01.T01:CDS 50.0%
!! TGATCGCGGTTATTTTGCGG+TGG - Chr6:93921417-93921436 None:intergenic 50.0%
GGTTTGCCGAGAAGTTAGGG+TGG + Chr6:93921572-93921591 MsG0680034750.01.T01:CDS 55.0%
TATTCCGGTGGAGGAGGTAG+TGG + Chr6:93921484-93921503 MsG0680034750.01.T01:CDS 55.0%
TGGAGGCTGTTTATCGCGGA+TGG - Chr6:93921364-93921383 None:intergenic 55.0%
! ATGGGAGGAGGAAGAGGTAC+CGG + Chr6:93921512-93921531 MsG0680034750.01.T01:CDS 55.0%
!! AGCTTCCAAGGCTTGCTGCA+TGG - Chr6:93921297-93921316 None:intergenic 55.0%
!! GAGGAGGTAGTGGAGGAGAT+GGG + Chr6:93921494-93921513 MsG0680034750.01.T01:CDS 55.0%
AGCCTCCAATGTGCAGCCTG+CGG + Chr6:93921376-93921395 MsG0680034750.01.T01:CDS 60.0%
CTTGGAAGCTACGCCACCGA+CGG + Chr6:93921307-93921326 MsG0680034750.01.T01:CDS 60.0%
GAGGAGATGGGAGGAGGAAG+AGG + Chr6:93921506-93921525 MsG0680034750.01.T01:CDS 60.0%
GGATGGCGATGAGTCGTCGA+TGG - Chr6:93921347-93921366 None:intergenic 60.0%
GGCACTCTCCCTTGTGATCG+CGG - Chr6:93921431-93921450 None:intergenic 60.0%
GGTGAATTCACCGCAGCCGT+CGG - Chr6:93921326-93921345 None:intergenic 60.0%
TCCTCCACTACCTCCTCCAC+CGG - Chr6:93921491-93921510 None:intergenic 60.0%
! GTAGTGGAGGAGATGGGAGG+AGG + Chr6:93921500-93921519 MsG0680034750.01.T01:CDS 60.0%
!! GAGGTAGTGGAGGAGATGGG+AGG + Chr6:93921497-93921516 MsG0680034750.01.T01:CDS 60.0%
!! GATCACAAGGGAGAGTGCCG+CGG + Chr6:93921432-93921451 MsG0680034750.01.T01:CDS 60.0%
!! GGAGGAGGTAGTGGAGGAGA+TGG + Chr6:93921493-93921512 MsG0680034750.01.T01:CDS 60.0%
AGCCGCAGGCTGCACATTGG+AGG - Chr6:93921381-93921400 None:intergenic 65.0%
ATCCGACATTGCAGCGGCCG+CGG - Chr6:93921452-93921471 None:intergenic 65.0%
CGTCGGTGGCGTAGCTTCCA+AGG - Chr6:93921309-93921328 None:intergenic 65.0%
GAAGTTCCGGTGACAGCCGC+AGG - Chr6:93921395-93921414 None:intergenic 65.0%
GAATTCACCGCAGCCGTCGG+TGG - Chr6:93921323-93921342 None:intergenic 65.0%
GACAGCCGCAGGCTGCACAT+TGG - Chr6:93921384-93921403 None:intergenic 65.0%
! TCCGGTGGAGGAGGTAGTGG+AGG + Chr6:93921487-93921506 MsG0680034750.01.T01:CDS 65.0%
AGCTACGCCACCGACGGCTG+CGG + Chr6:93921313-93921332 MsG0680034750.01.T01:CDS 70.0%
GTGCAGCCTGCGGCTGTCAC+CGG + Chr6:93921386-93921405 MsG0680034750.01.T01:CDS 70.0%
TGCCGCGGCCGCTGCAATGT+CGG + Chr6:93921447-93921466 MsG0680034750.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr6 gene 93921235 93921759 93921235 ID=MsG0680034750.01;Name=MsG0680034750.01
Chr6 mRNA 93921235 93921759 93921235 ID=MsG0680034750.01.T01;Parent=MsG0680034750.01;Name=MsG0680034750.01.T01;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|174
Chr6 exon 93921235 93921759 93921235 ID=MsG0680034750.01.T01:exon:5887;Parent=MsG0680034750.01.T01
Chr6 CDS 93921235 93921759 93921235 ID=MsG0680034750.01.T01:cds;Parent=MsG0680034750.01.T01
Gene Sequence

>MsG0680034750.01.T01

ATGTTATCATCTTCATCATCACATTCACAAAACTATATCTACAGAGAGTGTTTAAGAAACCATGCAGCAAGCCTTGGAAGCTACGCCACCGACGGCTGCGGTGAATTCACCATCGACGACTCATCGCCATCCGCGATAAACAGCCTCCAATGTGCAGCCTGCGGCTGTCACCGGAACTTCCACCGCAAAATAACCGCGATCACAAGGGAGAGTGCCGCGGCCGCTGCAATGTCGGATCAGTTGATGGAATATTCCGGTGGAGGAGGTAGTGGAGGAGATGGGAGGAGGAAGAGGTACCGGACGAAATTTACGCCGGATCAGAAGGAGAAGATGATGGGGTTTGCCGAGAAGTTAGGGTGGAAATTGCAGAGAAAAGAACTTGATGAAGAGATTGAAAGGTTTTGTGAAAGTGTTGGTGTGAGTAGACAAGTTTTTAAGGTGTGGATGCATAATCATAAGAATTCTTGTTTCTCAAATTCTTCTGATCCTTCTACTGGTAATGCTAATTCCTCTCTTACTCAGTAG

Protein sequence

>MsG0680034750.01.T01

MLSSSSSHSQNYIYRECLRNHAASLGSYATDGCGEFTIDDSSPSAINSLQCAACGCHRNFHRKITAITRESAAAAAMSDQLMEYSGGGGSGGDGRRKRYRTKFTPDQKEKMMGFAEKLGWKLQRKELDEEIERFCESVGVSRQVFKVWMHNHKNSCFSNSSDPSTGNANSSLTQ*