AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041403.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041403.01.T01 MTR_7g110310 100.000 142 0 0 1 142 253 394 1.32e-101 296
MsG0780041403.01.T01 MTR_4g123810 92.086 139 11 0 1 139 254 392 1.57e-81 245
MsG0780041403.01.T01 MTR_2g046710 94.853 136 7 0 1 136 254 389 2.32e-81 244
MsG0780041403.01.T01 MTR_1g063060 86.765 136 18 0 1 136 252 387 9.58e-74 225
MsG0780041403.01.T01 MTR_7g102120 86.765 136 18 0 1 136 252 387 1.15e-73 225
MsG0780041403.01.T01 MTR_1g063060 86.765 136 18 0 1 136 270 405 1.61e-73 225
MsG0780041403.01.T02 MTR_7g110310 100.000 47 0 0 1 47 348 394 1.03e-28 104
MsG0780041403.01.T02 MTR_4g123810 88.636 44 5 0 1 44 349 392 9.99e-24 90.9
MsG0780041403.01.T02 MTR_2g046710 92.683 41 3 0 1 41 349 389 1.44e-22 87.4
MsG0780041403.01.T02 MTR_7g102120 82.927 41 7 0 1 41 347 387 8.02e-19 77.4
MsG0780041403.01.T02 MTR_1g063060 80.488 41 8 0 1 41 365 405 4.77e-18 75.1
MsG0780041403.01.T02 MTR_1g063060 80.488 41 8 0 1 41 347 387 5.18e-18 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041403.01.T01 AT1G02500 90.141 142 14 0 1 142 252 393 1.80e-82 248
MsG0780041403.01.T01 AT1G02500 90.141 142 14 0 1 142 252 393 1.80e-82 248
MsG0780041403.01.T01 AT4G01850 90.141 142 14 0 1 142 252 393 2.75e-82 247
MsG0780041403.01.T01 AT4G01850 90.141 142 14 0 1 142 252 393 2.75e-82 247
MsG0780041403.01.T01 AT3G17390 91.549 142 12 0 1 142 252 393 9.68e-81 243
MsG0780041403.01.T01 AT2G36880 88.235 136 16 0 1 136 252 387 4.28e-75 229
MsG0780041403.01.T01 AT2G36880 88.235 136 16 0 1 136 252 387 4.28e-75 229
MsG0780041403.01.T02 AT1G02500 93.617 47 3 0 1 47 347 393 4.09e-27 100
MsG0780041403.01.T02 AT1G02500 93.617 47 3 0 1 47 347 393 4.09e-27 100
MsG0780041403.01.T02 AT4G01850 91.489 47 4 0 1 47 347 393 3.47e-26 97.4
MsG0780041403.01.T02 AT4G01850 91.489 47 4 0 1 47 347 393 3.47e-26 97.4
MsG0780041403.01.T02 AT3G17390 87.234 47 6 0 1 47 347 393 6.55e-24 91.3
MsG0780041403.01.T02 AT2G36880 90.244 41 4 0 1 41 347 387 1.17e-20 82.4
MsG0780041403.01.T02 AT2G36880 90.244 41 4 0 1 41 347 387 1.17e-20 82.4

Find 55 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCTTAAGGTCCAAGTTTA+TGG 0.138584 7:-90532709 None:intergenic
TTTCTTAAGACAGCTGCTTA+TGG 0.284960 7:+90532747 MsG0780041403.01.T01:CDS
AAGATTATCATTGATACTTA+TGG 0.306222 7:+90532420 MsG0780041403.01.T01:CDS
CATTGATACTTATGGTGGTT+GGG 0.317021 7:+90532428 MsG0780041403.01.T01:CDS
CCACTTCTGTCAACCTTGGT+TGG 0.360492 7:-90532486 None:intergenic
TGCTGTTGCCACCCCTCTTA+AGG 0.364997 7:-90532722 None:intergenic
CACTTCTGTCAACCTTGGTT+GGG 0.374639 7:-90532485 None:intergenic
TCAGTCTTTGTTGACAGTTA+TGG 0.391107 7:+90532612 MsG0780041403.01.T01:CDS
AAGTTTATGGTGATCATTCC+AGG 0.391766 7:-90532696 None:intergenic
CCATAAACTTGGACCTTAAG+AGG 0.398540 7:+90532709 MsG0780041403.01.T01:CDS
CACGGTGGAGGTGCCTTCTC+AGG 0.402997 7:+90532456 MsG0780041403.01.T01:CDS
TTTGTTGACAGTTATGGAAC+TGG 0.419936 7:+90532618 MsG0780041403.01.T01:CDS
GGTTGGGGTGCTCACGGTGG+AGG 0.425538 7:+90532444 MsG0780041403.01.T01:CDS
GGTTGGGTCCTTTCCTGAGA+AGG 0.428342 7:-90532469 None:intergenic
TCATTGATACTTATGGTGGT+TGG 0.441161 7:+90532427 MsG0780041403.01.T01:CDS
ACAACTTCCCATGTGAAGTC+AGG 0.445873 7:-90532789 None:intergenic
GAGAATTTCGACTTCAGACC+TGG 0.446842 7:+90532678 MsG0780041403.01.T01:CDS
ACAAAGACTGACAAAGGTTC+AGG 0.472926 7:-90532600 None:intergenic
ATTTGAAGAATCTCCCTGTC+AGG 0.484553 7:-90532648 None:intergenic
CTGCTTATGGACACTTTGGA+AGG 0.487231 7:+90532760 MsG0780041403.01.T01:CDS
ACAGCTGCTTATGGACACTT+TGG 0.494709 7:+90532756 MsG0780041403.01.T01:CDS
TGGAATGATCACCATAAACT+TGG 0.495635 7:+90532698 MsG0780041403.01.T01:CDS
TTAAGAGGGGTGGCAACAGC+AGG 0.499577 7:+90532724 MsG0780041403.01.T01:CDS
TATGGTGGTTGGGGTGCTCA+CGG 0.517736 7:+90532438 MsG0780041403.01.T01:CDS
ATTGATACTTATGGTGGTTG+GGG 0.520128 7:+90532429 MsG0780041403.01.T01:CDS
GAAGTGGTGCCTATGTTGTA+AGG 0.524030 7:+90532502 MsG0780041403.01.T01:CDS
TGAGGCTTCTCCCACTTGAG+TGG 0.529340 7:-90532816 None:intergenic
GTTCAAGTTTCATACGCCAT+TGG 0.533659 7:+90532573 MsG0780041403.01.T01:CDS
ACCGTTGGCCACGATACTCT+TGG 0.534046 7:-90532532 None:intergenic
CAACACAATTCTTAAGCTTG+AGG 0.534059 7:-90532834 None:intergenic
GGGATGATCCTGACTTCACA+TGG 0.535224 7:+90532781 MsG0780041403.01.T01:CDS
TGGTGCCTATGTTGTAAGGC+AGG 0.539064 7:+90532506 MsG0780041403.01.T01:CDS
GGCACCACTTCTGTCAACCT+TGG 0.561156 7:-90532490 None:intergenic
CATAAACTTGGACCTTAAGA+GGG 0.561791 7:+90532710 MsG0780041403.01.T01:CDS
GAACTGGAAAGATTCCTGAC+AGG 0.562498 7:+90532634 MsG0780041403.01.T01:CDS
GGCCGCCTGCCTTACAACAT+AGG 0.573580 7:-90532511 None:intergenic
GGAGATTCTTCAAATTGTGA+AGG 0.577415 7:+90532656 MsG0780041403.01.T01:CDS
ATTATCATTGATACTTATGG+TGG 0.577853 7:+90532423 MsG0780041403.01.T01:CDS
AACTTGGACCTTAAGAGGGG+TGG 0.600140 7:+90532714 MsG0780041403.01.T01:CDS
TGCTTATGGACACTTTGGAA+GGG 0.601939 7:+90532761 MsG0780041403.01.T01:CDS
GCCAAGAGTATCGTGGCCAA+CGG 0.604104 7:+90532531 MsG0780041403.01.T01:CDS
AAGTTGTGAAACCACTCAAG+TGG 0.610837 7:+90532805 MsG0780041403.01.T01:CDS
CCAACCAAGGTTGACAGAAG+TGG 0.610892 7:+90532486 MsG0780041403.01.T01:CDS
TGGAGGTGCCTTCTCAGGAA+AGG 0.616557 7:+90532461 MsG0780041403.01.T01:CDS
TGCCTATGTTGTAAGGCAGG+CGG 0.619125 7:+90532509 MsG0780041403.01.T01:CDS
GCATCTGCGAGCAAGACCGT+TGG 0.622592 7:-90532547 None:intergenic
CTGTCAACAAAGACTGACAA+AGG 0.630556 7:-90532606 None:intergenic
CAAAGGTTCAGGCACACCAA+TGG 0.637977 7:-90532589 None:intergenic
AACTGGAAAGATTCCTGACA+GGG 0.640690 7:+90532635 MsG0780041403.01.T01:CDS
GGTGGTTGGGGTGCTCACGG+TGG 0.644744 7:+90532441 MsG0780041403.01.T01:CDS
AGTTGTGAAACCACTCAAGT+GGG 0.645098 7:+90532806 MsG0780041403.01.T01:CDS
CTCAGGAAAGGACCCAACCA+AGG 0.650893 7:+90532473 MsG0780041403.01.T01:CDS
GGATGATCCTGACTTCACAT+GGG 0.698633 7:+90532782 MsG0780041403.01.T01:CDS
GCAGGCGGCCAAGAGTATCG+TGG 0.699100 7:+90532524 MsG0780041403.01.T01:CDS
ATAAACTTGGACCTTAAGAG+GGG 0.707857 7:+90532711 MsG0780041403.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGATTATCATTGATACTTA+TGG + Chr7:90532420-90532439 MsG0780041403.01.T01:CDS 20.0%
! ATTATCATTGATACTTATGG+TGG + Chr7:90532423-90532442 MsG0780041403.01.T01:CDS 25.0%
AAGTTTATGGTGATCATTCC+AGG - Chr7:90532699-90532718 None:intergenic 35.0%
ATAAACTTGGACCTTAAGAG+GGG + Chr7:90532711-90532730 MsG0780041403.01.T01:CDS 35.0%
ATTGATACTTATGGTGGTTG+GGG + Chr7:90532429-90532448 MsG0780041403.01.T01:CDS 35.0%
CATAAACTTGGACCTTAAGA+GGG + Chr7:90532710-90532729 MsG0780041403.01.T01:CDS 35.0%
CATTGATACTTATGGTGGTT+GGG + Chr7:90532428-90532447 MsG0780041403.01.T01:CDS 35.0%
GGAGATTCTTCAAATTGTGA+AGG + Chr7:90532656-90532675 MsG0780041403.01.T01:CDS 35.0%
TCAGTCTTTGTTGACAGTTA+TGG + Chr7:90532612-90532631 MsG0780041403.01.T01:CDS 35.0%
TCATTGATACTTATGGTGGT+TGG + Chr7:90532427-90532446 MsG0780041403.01.T01:CDS 35.0%
TGGAATGATCACCATAAACT+TGG + Chr7:90532698-90532717 MsG0780041403.01.T01:CDS 35.0%
TTTCTTAAGACAGCTGCTTA+TGG + Chr7:90532747-90532766 MsG0780041403.01.T01:CDS 35.0%
TTTGTTGACAGTTATGGAAC+TGG + Chr7:90532618-90532637 MsG0780041403.01.T01:CDS 35.0%
AACTGGAAAGATTCCTGACA+GGG + Chr7:90532635-90532654 MsG0780041403.01.T01:CDS 40.0%
AAGTTGTGAAACCACTCAAG+TGG + Chr7:90532805-90532824 MsG0780041403.01.T01:CDS 40.0%
ACAAAGACTGACAAAGGTTC+AGG - Chr7:90532603-90532622 None:intergenic 40.0%
AGTTGTGAAACCACTCAAGT+GGG + Chr7:90532806-90532825 MsG0780041403.01.T01:CDS 40.0%
ATTTGAAGAATCTCCCTGTC+AGG - Chr7:90532651-90532670 None:intergenic 40.0%
CCATAAACTTGGACCTTAAG+AGG + Chr7:90532709-90532728 MsG0780041403.01.T01:CDS 40.0%
CCTCTTAAGGTCCAAGTTTA+TGG - Chr7:90532712-90532731 None:intergenic 40.0%
CTGTCAACAAAGACTGACAA+AGG - Chr7:90532609-90532628 None:intergenic 40.0%
! GTTCAAGTTTCATACGCCAT+TGG + Chr7:90532573-90532592 MsG0780041403.01.T01:CDS 40.0%
! TGCTTATGGACACTTTGGAA+GGG + Chr7:90532761-90532780 MsG0780041403.01.T01:CDS 40.0%
ACAACTTCCCATGTGAAGTC+AGG - Chr7:90532792-90532811 None:intergenic 45.0%
ACAGCTGCTTATGGACACTT+TGG + Chr7:90532756-90532775 MsG0780041403.01.T01:CDS 45.0%
CACTTCTGTCAACCTTGGTT+GGG - Chr7:90532488-90532507 None:intergenic 45.0%
CTGCTTATGGACACTTTGGA+AGG + Chr7:90532760-90532779 MsG0780041403.01.T01:CDS 45.0%
GAACTGGAAAGATTCCTGAC+AGG + Chr7:90532634-90532653 MsG0780041403.01.T01:CDS 45.0%
GAAGTGGTGCCTATGTTGTA+AGG + Chr7:90532502-90532521 MsG0780041403.01.T01:CDS 45.0%
GAGAATTTCGACTTCAGACC+TGG + Chr7:90532678-90532697 MsG0780041403.01.T01:CDS 45.0%
GGATGATCCTGACTTCACAT+GGG + Chr7:90532782-90532801 MsG0780041403.01.T01:CDS 45.0%
AACTTGGACCTTAAGAGGGG+TGG + Chr7:90532714-90532733 MsG0780041403.01.T01:CDS 50.0%
CAAAGGTTCAGGCACACCAA+TGG - Chr7:90532592-90532611 None:intergenic 50.0%
CCAACCAAGGTTGACAGAAG+TGG + Chr7:90532486-90532505 MsG0780041403.01.T01:CDS 50.0%
CCACTTCTGTCAACCTTGGT+TGG - Chr7:90532489-90532508 None:intergenic 50.0%
GGGATGATCCTGACTTCACA+TGG + Chr7:90532781-90532800 MsG0780041403.01.T01:CDS 50.0%
TGCCTATGTTGTAAGGCAGG+CGG + Chr7:90532509-90532528 MsG0780041403.01.T01:CDS 50.0%
TGGTGCCTATGTTGTAAGGC+AGG + Chr7:90532506-90532525 MsG0780041403.01.T01:CDS 50.0%
ACCGTTGGCCACGATACTCT+TGG - Chr7:90532535-90532554 None:intergenic 55.0%
CTCAGGAAAGGACCCAACCA+AGG + Chr7:90532473-90532492 MsG0780041403.01.T01:CDS 55.0%
GGCACCACTTCTGTCAACCT+TGG - Chr7:90532493-90532512 None:intergenic 55.0%
GGTTGGGTCCTTTCCTGAGA+AGG - Chr7:90532472-90532491 None:intergenic 55.0%
TGAGGCTTCTCCCACTTGAG+TGG - Chr7:90532819-90532838 None:intergenic 55.0%
TGCTGTTGCCACCCCTCTTA+AGG - Chr7:90532725-90532744 None:intergenic 55.0%
TTAAGAGGGGTGGCAACAGC+AGG + Chr7:90532724-90532743 MsG0780041403.01.T01:CDS 55.0%
! TATGGTGGTTGGGGTGCTCA+CGG + Chr7:90532438-90532457 MsG0780041403.01.T01:CDS 55.0%
!! GCCAAGAGTATCGTGGCCAA+CGG + Chr7:90532531-90532550 MsG0780041403.01.T01:CDS 55.0%
!! TGGAGGTGCCTTCTCAGGAA+AGG + Chr7:90532461-90532480 MsG0780041403.01.T01:CDS 55.0%
GCATCTGCGAGCAAGACCGT+TGG - Chr7:90532550-90532569 None:intergenic 60.0%
GGCCGCCTGCCTTACAACAT+AGG - Chr7:90532514-90532533 None:intergenic 60.0%
!! CACGGTGGAGGTGCCTTCTC+AGG + Chr7:90532456-90532475 MsG0780041403.01.T01:CDS 65.0%
!! GCAGGCGGCCAAGAGTATCG+TGG + Chr7:90532524-90532543 MsG0780041403.01.T01:CDS 65.0%
! GGTGGTTGGGGTGCTCACGG+TGG + Chr7:90532441-90532460 MsG0780041403.01.T01:CDS 70.0%
! GGTTGGGGTGCTCACGGTGG+AGG + Chr7:90532444-90532463 MsG0780041403.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr7 gene 90532417 90532845 90532417 ID=MsG0780041403.01;Name=MsG0780041403.01
Chr7 mRNA 90532417 90532845 90532417 ID=MsG0780041403.01.T01;Parent=MsG0780041403.01;Name=MsG0780041403.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|142
Chr7 exon 90532417 90532845 90532417 ID=MsG0780041403.01.T01:exon:21255;Parent=MsG0780041403.01.T01
Chr7 CDS 90532417 90532845 90532417 ID=MsG0780041403.01.T01:cds;Parent=MsG0780041403.01.T01
Chr7 mRNA 90532702 90532845 90532702 ID=MsG0780041403.01.T02;Parent=MsG0780041403.01;Name=MsG0780041403.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|47
Chr7 exon 90532702 90532845 90532702 ID=MsG0780041403.01.T02:exon:21256;Parent=MsG0780041403.01.T02
Chr7 CDS 90532702 90532845 90532702 ID=MsG0780041403.01.T02:cds;Parent=MsG0780041403.01.T02
Chr7 mRNA 90532439 90532522 90532439 ID=MsG0780041403.01.T03;Parent=MsG0780041403.01;Name=MsG0780041403.01.T03;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|27
Chr7 exon 90532439 90532522 90532439 ID=MsG0780041403.01.T03:exon:21257;Parent=MsG0780041403.01.T03
Chr7 CDS 90532439 90532522 90532439 ID=MsG0780041403.01.T03:cds;Parent=MsG0780041403.01.T03
Gene Sequence

>MsG0780041403.01.T03

ATGGTGGTTGGGGTGCTCACGGTGGAGGTGCCTTCTCAGGAAAGGACCCAACCAAGGTTGACAGAAGTGGTGCCTATGTTGTAA

>MsG0780041403.01.T01

AGAAAGATTATCATTGATACTTATGGTGGTTGGGGTGCTCACGGTGGAGGTGCCTTCTCAGGAAAGGACCCAACCAAGGTTGACAGAAGTGGTGCCTATGTTGTAAGGCAGGCGGCCAAGAGTATCGTGGCCAACGGTCTTGCTCGCAGATGCATTGTTCAAGTTTCATACGCCATTGGTGTGCCTGAACCTTTGTCAGTCTTTGTTGACAGTTATGGAACTGGAAAGATTCCTGACAGGGAGATTCTTCAAATTGTGAAGGAGAATTTCGACTTCAGACCTGGAATGATCACCATAAACTTGGACCTTAAGAGGGGTGGCAACAGCAGGTTTCTTAAGACAGCTGCTTATGGACACTTTGGAAGGGATGATCCTGACTTCACATGGGAAGTTGTGAAACCACTCAAGTGGGAGAAGCCTCAAGCTTAA

>MsG0780041403.01.T02

ATGATCACCATAAACTTGGACCTTAAGAGGGGTGGCAACAGCAGGTTTCTTAAGACAGCTGCTTATGGACACTTTGGAAGGGATGATCCTGACTTCACATGGGAAGTTGTGAAACCACTCAAGTGGGAGAAGCCTCAAGCTTAA

Protein sequence

>MsG0780041403.01.T03

MVVGVLTVEVPSQERTQPRLTEVVPML*

>MsG0780041403.01.T01

RKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYVVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDREILQIVKENFDFRPGMITINLDLKRGGNSRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA*

>MsG0780041403.01.T02

MITINLDLKRGGNSRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA*